Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051770.t1 | XP_013450261.1 | 96.1 | 207 | 8 | 0 | 1 | 207 | 267 | 473 | 1.30E-104 | 389 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051770.t1 | E9L7A5 | 85.1 | 201 | 30 | 0 | 1 | 201 | 273 | 473 | 3.5e-93 | 342.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051770.t1 | A0A072U3J0 | 96.1 | 207 | 8 | 0 | 1 | 207 | 267 | 473 | 9.6e-105 | 389.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051770 | MS.gene051772 | 0.836045 | 1.18E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051770.t1 | MTR_7g106830 | 96.135 | 207 | 8 | 0 | 1 | 207 | 267 | 473 | 6.97e-144 | 409 |
| MS.gene051770.t1 | MTR_8g091280 | 90.821 | 207 | 19 | 0 | 1 | 207 | 269 | 475 | 3.70e-135 | 387 |
| MS.gene051770.t1 | MTR_1807s0010 | 36.923 | 195 | 119 | 2 | 1 | 194 | 201 | 392 | 2.84e-33 | 123 |
| MS.gene051770.t1 | MTR_3g072670 | 27.094 | 203 | 129 | 6 | 1 | 198 | 255 | 443 | 5.16e-19 | 84.7 |
| MS.gene051770.t1 | MTR_4g011820 | 29.384 | 211 | 128 | 5 | 1 | 207 | 201 | 394 | 1.95e-18 | 82.8 |
| MS.gene051770.t1 | MTR_1g087760 | 30.841 | 214 | 118 | 7 | 2 | 198 | 194 | 394 | 2.02e-18 | 82.8 |
| MS.gene051770.t1 | MTR_3g072680 | 27.586 | 203 | 128 | 6 | 1 | 198 | 212 | 400 | 2.07e-18 | 82.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051770.t1 | AT2G22250 | 75.622 | 201 | 49 | 0 | 1 | 201 | 222 | 422 | 1.74e-108 | 318 |
| MS.gene051770.t1 | AT2G22250 | 75.622 | 201 | 49 | 0 | 1 | 201 | 269 | 469 | 8.78e-108 | 318 |
| MS.gene051770.t1 | AT2G22250 | 75.622 | 201 | 49 | 0 | 1 | 201 | 269 | 469 | 8.78e-108 | 318 |
| MS.gene051770.t1 | AT1G77670 | 28.571 | 203 | 126 | 6 | 1 | 198 | 248 | 436 | 1.01e-21 | 92.4 |
| MS.gene051770.t1 | AT1G80360 | 27.700 | 213 | 128 | 7 | 1 | 207 | 201 | 393 | 2.02e-15 | 74.3 |
| MS.gene051770.t1 | AT1G80360 | 27.700 | 213 | 128 | 7 | 1 | 207 | 201 | 393 | 2.02e-15 | 74.3 |
| MS.gene051770.t1 | AT1G80360 | 27.700 | 213 | 128 | 7 | 1 | 207 | 201 | 393 | 2.02e-15 | 74.3 |
| MS.gene051770.t1 | AT1G80360 | 27.700 | 213 | 128 | 7 | 1 | 207 | 201 | 393 | 2.02e-15 | 74.3 |
Find 45 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGATTTCAGCTCCTATTA+TGG | 0.261186 | 7.3:-7080939 | MS.gene051770:CDS |
| TTCAGCTTCTCTTCCATAAT+AGG | 0.283252 | 7.3:+7080926 | None:intergenic |
| GGAAGAGAAGCTGAAGGTTT+TGG | 0.300530 | 7.3:-7080918 | MS.gene051770:CDS |
| TGGCGGCTTGGTTATATTGC+AGG | 0.312104 | 7.3:-7081501 | MS.gene051770:CDS |
| ATGCTGAAATAAAATGTTTC+GGG | 0.353525 | 7.3:+7081478 | None:intergenic |
| CTTGTGCCAGGGAGTGCATC+TGG | 0.364335 | 7.3:-7080728 | MS.gene051770:CDS |
| TGTCAATATCTGTTGCATAA+AGG | 0.366152 | 7.3:-7080870 | MS.gene051770:CDS |
| CATGCTGAAATAAAATGTTT+CGG | 0.387560 | 7.3:+7081477 | None:intergenic |
| CTACAGGTCGCACTTGTGCC+AGG | 0.411505 | 7.3:-7080740 | MS.gene051770:intron |
| ACAAGTGCAGCTGAGGTAAG+AGG | 0.413408 | 7.3:+7080620 | None:intergenic |
| CTGCAGCCCTTTGCAATGAC+TGG | 0.426334 | 7.3:-7081525 | MS.gene051770:intron |
| AAAGCTGCAGTTGCTGCATC+AGG | 0.433618 | 7.3:-7081189 | MS.gene051770:CDS |
| GCAATGACTGGTTGGCGGCT+TGG | 0.436164 | 7.3:-7081513 | MS.gene051770:CDS |
| GCAAAGCTAGTGTGTGTTGC+TGG | 0.444438 | 7.3:+7081746 | None:intergenic |
| ATGTGGAAAGATTCAAAGTC+AGG | 0.449025 | 7.3:-7081457 | MS.gene051770:intron |
| AACCTTAGAAAATCCATTCA+CGG | 0.462409 | 7.3:+7081691 | None:intergenic |
| GCAGTTGCTGCATCAGGACT+AGG | 0.466446 | 7.3:-7081183 | MS.gene051770:CDS |
| TCTCTCTACAGCTGCCTGTA+GGG | 0.480917 | 7.3:+7080661 | None:intergenic |
| GATAAAACGCTAACCGTGAA+TGG | 0.483375 | 7.3:-7081704 | MS.gene051770:CDS |
| GCATCAGGACTAGGCTATGC+TGG | 0.488598 | 7.3:-7081174 | MS.gene051770:CDS |
| AAGGATTCACACCTGTGGTT+CGG | 0.490759 | 7.3:+7081059 | None:intergenic |
| TAGAGAACGAAGGAATTACT+TGG | 0.497722 | 7.3:-7081118 | MS.gene051770:CDS |
| CGCCAACCAGTCATTGCAAA+GGG | 0.498469 | 7.3:+7081519 | None:intergenic |
| CCTTTGCAATGACTGGTTGG+CGG | 0.506213 | 7.3:-7081518 | MS.gene051770:CDS |
| TCGTCTCCAGATGCACTCCC+TGG | 0.507721 | 7.3:+7080722 | None:intergenic |
| ACTAGCTTTGCATCTTTACC+TGG | 0.509818 | 7.3:-7081734 | MS.gene051770:CDS |
| TGCATCTTTACCTGGAATGT+GGG | 0.520471 | 7.3:-7081726 | MS.gene051770:CDS |
| TTGCATCTTTACCTGGAATG+TGG | 0.521651 | 7.3:-7081727 | MS.gene051770:CDS |
| AGCATCTCTTCCTACCCTAC+AGG | 0.523160 | 7.3:-7080675 | MS.gene051770:CDS |
| AGCCCTTTGCAATGACTGGT+TGG | 0.531466 | 7.3:-7081521 | MS.gene051770:CDS |
| CAAGTGCAGCTGAGGTAAGA+GGG | 0.556805 | 7.3:+7080621 | None:intergenic |
| ATATCTGTTGCATAAAGGCC+AGG | 0.563043 | 7.3:-7080865 | MS.gene051770:intron |
| TACAGGTCGCACTTGTGCCA+GGG | 0.565282 | 7.3:-7080739 | MS.gene051770:intron |
| TTCTCTCTACAGCTGCCTGT+AGG | 0.565977 | 7.3:+7080660 | None:intergenic |
| TCTACAGCTGCCTGTAGGGT+AGG | 0.574697 | 7.3:+7080665 | None:intergenic |
| AGCTTTCTGAGATATACTAC+TGG | 0.576070 | 7.3:+7081206 | None:intergenic |
| CCGCCAACCAGTCATTGCAA+AGG | 0.598202 | 7.3:+7081518 | None:intergenic |
| TGTTGAAATATCCGAACCAC+AGG | 0.604423 | 7.3:-7081070 | MS.gene051770:intron |
| TATTATGGAAGAGAAGCTGA+AGG | 0.610603 | 7.3:-7080924 | MS.gene051770:CDS |
| ACACTAGACAAGTGCAGCTG+AGG | 0.616702 | 7.3:+7080613 | None:intergenic |
| ACTAGGCTATGCTGGTGGAG+AGG | 0.620996 | 7.3:-7081166 | MS.gene051770:CDS |
| TTCTCTAAATGCTTTCACCA+TGG | 0.638071 | 7.3:+7081134 | None:intergenic |
| TGGAGAGGTTGTTTCTACCA+TGG | 0.638313 | 7.3:-7081151 | MS.gene051770:CDS |
| TCAGGACTAGGCTATGCTGG+TGG | 0.663983 | 7.3:-7081171 | MS.gene051770:CDS |
| TGAAAGCATTTAGAGAACGA+AGG | 0.676649 | 7.3:-7081128 | MS.gene051770:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTGATATTATATTATATG+CGG | - | chr7.3:7081126-7081145 | MS.gene051770:CDS | 10.0% |
| !! | TATAAATTATGAAATAAACT+TGG | - | chr7.3:7080824-7080843 | MS.gene051770:intron | 10.0% |
| !!! | TTATTGATTTTAGTTTACTT+CGG | - | chr7.3:7081169-7081188 | MS.gene051770:CDS | 15.0% |
| !! | AAAAAAAAATTAGAAGCCTT+AGG | + | chr7.3:7080855-7080874 | None:intergenic | 20.0% |
| !! | ACGAATATGGTTAAAATTAA+AGG | + | chr7.3:7081061-7081080 | None:intergenic | 20.0% |
| !! | CGAATATGGTTAAAATTAAA+GGG | + | chr7.3:7081060-7081079 | None:intergenic | 20.0% |
| !! | TCATAATTTATAACCATACT+AGG | + | chr7.3:7080816-7080835 | None:intergenic | 20.0% |
| !!! | TATTGATTTTAGTTTACTTC+GGG | - | chr7.3:7081170-7081189 | MS.gene051770:CDS | 20.0% |
| ! | ATAAGATGAAGCTTAGAATA+CGG | + | chr7.3:7080996-7081015 | None:intergenic | 25.0% |
| ! | ATGCTGAAATAAAATGTTTC+GGG | + | chr7.3:7080926-7080945 | None:intergenic | 25.0% |
| ! | CATGCTGAAATAAAATGTTT+CGG | + | chr7.3:7080927-7080946 | None:intergenic | 25.0% |
| ! | TCACTCTTAAATAACGAATA+TGG | + | chr7.3:7081074-7081093 | None:intergenic | 25.0% |
| !!! | AAACATTTTATTTCAGCATG+TGG | - | chr7.3:7080927-7080946 | MS.gene051770:CDS | 25.0% |
| !!! | ATTGATTTTAGTTTACTTCG+GGG | - | chr7.3:7081171-7081190 | MS.gene051770:CDS | 25.0% |
| AACCTTAGAAAATCCATTCA+CGG | + | chr7.3:7080713-7080732 | None:intergenic | 30.0% | |
| AATAAACTTGGATGATCCTA+AGG | - | chr7.3:7080836-7080855 | MS.gene051770:intron | 30.0% | |
| GAGTATGCAAATAAACTAGA+AGG | + | chr7.3:7081364-7081383 | None:intergenic | 30.0% | |
| TGTCAATATCTGTTGCATAA+AGG | - | chr7.3:7081531-7081550 | MS.gene051770:intron | 30.0% | |
| TTATGTTGCATATCCTAGTA+TGG | - | chr7.3:7080800-7080819 | MS.gene051770:intron | 30.0% | |
| ! | ATTGATTTCAGCTCCTATTA+TGG | - | chr7.3:7081462-7081481 | MS.gene051770:CDS | 30.0% |
| !! | AAAAGTTTTAGCGAAATCGA+TGG | - | chr7.3:7081308-7081327 | MS.gene051770:intron | 30.0% |
| !!! | TTGATTTTAGTTTACTTCGG+GGG | - | chr7.3:7081172-7081191 | MS.gene051770:CDS | 30.0% |
| AGCGAACATAAAAGAGCAAA+AGG | + | chr7.3:7081386-7081405 | None:intergenic | 35.0% | |
| ATGTGGAAAGATTCAAAGTC+AGG | - | chr7.3:7080944-7080963 | MS.gene051770:CDS | 35.0% | |
| CAGAAAGAGAACATGACATA+TGG | + | chr7.3:7080739-7080758 | None:intergenic | 35.0% | |
| CATATGTCATGTTCTCTTTC+TGG | - | chr7.3:7080737-7080756 | MS.gene051770:CDS | 35.0% | |
| GAAACTTCTGTGCATACATT+TGG | - | chr7.3:7081101-7081120 | MS.gene051770:CDS | 35.0% | |
| TAACATTGTGTTTCGTCTAC+AGG | - | chr7.3:7081645-7081664 | MS.gene051770:intron | 35.0% | |
| TAGAGAACGAAGGAATTACT+TGG | - | chr7.3:7081283-7081302 | MS.gene051770:intron | 35.0% | |
| TATTATGGAAGAGAAGCTGA+AGG | - | chr7.3:7081477-7081496 | MS.gene051770:CDS | 35.0% | |
| TGTCATTATATGAGCATACC+TGG | + | chr7.3:7081557-7081576 | None:intergenic | 35.0% | |
| TTCAGCTTCTCTTCCATAAT+AGG | + | chr7.3:7081478-7081497 | None:intergenic | 35.0% | |
| TTCTCTAAATGCTTTCACCA+TGG | + | chr7.3:7081270-7081289 | None:intergenic | 35.0% | |
| ! | ATACTTTATTTCCGCTTGCA+GGG | - | chr7.3:7081423-7081442 | MS.gene051770:intron | 35.0% |
| ! | TATACTTTATTTCCGCTTGC+AGG | - | chr7.3:7081422-7081441 | MS.gene051770:intron | 35.0% |
| !! | AACCGTGAATGGATTTTCTA+AGG | - | chr7.3:7080708-7080727 | MS.gene051770:CDS | 35.0% |
| !! | AGCTTTCTGAGATATACTAC+TGG | + | chr7.3:7081198-7081217 | None:intergenic | 35.0% |
| !! | TGAAAGCATTTAGAGAACGA+AGG | - | chr7.3:7081273-7081292 | MS.gene051770:intron | 35.0% |
| !! | TGAAGGTTTTGGCAAAATTG+AGG | - | chr7.3:7081494-7081513 | MS.gene051770:CDS | 35.0% |
| AATAAAATGCTCCCTGCAAG+CGG | + | chr7.3:7081437-7081456 | None:intergenic | 40.0% | |
| ATATCTGTTGCATAAAGGCC+AGG | - | chr7.3:7081536-7081555 | MS.gene051770:intron | 40.0% | |
| CTTGATTTCCTCTTGCTACA+AGG | - | chr7.3:7081016-7081035 | MS.gene051770:intron | 40.0% | |
| GATAAAACGCTAACCGTGAA+TGG | - | chr7.3:7080697-7080716 | MS.gene051770:CDS | 40.0% | |
| TGCATCTTTACCTGGAATGT+GGG | - | chr7.3:7080675-7080694 | MS.gene051770:CDS | 40.0% | |
| TGTTGAAATATCCGAACCAC+AGG | - | chr7.3:7081331-7081350 | MS.gene051770:intron | 40.0% | |
| TTGCATCTTTACCTGGAATG+TGG | - | chr7.3:7080674-7080693 | MS.gene051770:CDS | 40.0% | |
| !! | ACTAGCTTTGCATCTTTACC+TGG | - | chr7.3:7080667-7080686 | MS.gene051770:CDS | 40.0% |
| AAGGATTCACACCTGTGGTT+CGG | + | chr7.3:7081345-7081364 | None:intergenic | 45.0% | |
| ACTAGAAGGATTCACACCTG+TGG | + | chr7.3:7081350-7081369 | None:intergenic | 45.0% | |
| CTTGCTACAAGGCATGCAAA+AGG | - | chr7.3:7081027-7081046 | MS.gene051770:intron | 45.0% | |
| TTTGCATGCCTTGTAGCAAG+AGG | + | chr7.3:7081027-7081046 | None:intergenic | 45.0% | |
| ! | AGCGTTTTATCCCACATTCC+AGG | + | chr7.3:7080688-7080707 | None:intergenic | 45.0% |
| ! | GGAAGAGAAGCTGAAGGTTT+TGG | - | chr7.3:7081483-7081502 | MS.gene051770:CDS | 45.0% |
| !! | TGGAGAGGTTGTTTCTACCA+TGG | - | chr7.3:7081250-7081269 | MS.gene051770:intron | 45.0% |
| AAAGCTGCAGTTGCTGCATC+AGG | - | chr7.3:7081212-7081231 | MS.gene051770:CDS | 50.0% | |
| ACAAGTGCAGCTGAGGTAAG+AGG | + | chr7.3:7081784-7081803 | None:intergenic | 50.0% | |
| AGCATCTCTTCCTACCCTAC+AGG | - | chr7.3:7081726-7081745 | MS.gene051770:CDS | 50.0% | |
| AGCCCTTTGCAATGACTGGT+TGG | - | chr7.3:7080880-7080899 | MS.gene051770:CDS | 50.0% | |
| CAAGTGCAGCTGAGGTAAGA+GGG | + | chr7.3:7081783-7081802 | None:intergenic | 50.0% | |
| CGCCAACCAGTCATTGCAAA+GGG | + | chr7.3:7080885-7080904 | None:intergenic | 50.0% | |
| GCAAAGCTAGTGTGTGTTGC+TGG | + | chr7.3:7080658-7080677 | None:intergenic | 50.0% | |
| TACCTCAGCTGCACTTGTCT+AGG | - | chr7.3:7081786-7081805 | MS.gene051770:CDS | 50.0% | |
| TCTCTCTACAGCTGCCTGTA+GGG | + | chr7.3:7081743-7081762 | None:intergenic | 50.0% | |
| TTCTCTCTACAGCTGCCTGT+AGG | + | chr7.3:7081744-7081763 | None:intergenic | 50.0% | |
| ! | CCTTTGCAATGACTGGTTGG+CGG | - | chr7.3:7080883-7080902 | MS.gene051770:CDS | 50.0% |
| !! | TGGCGGCTTGGTTATATTGC+AGG | - | chr7.3:7080900-7080919 | MS.gene051770:CDS | 50.0% |
| ACTAGGCTATGCTGGTGGAG+AGG | - | chr7.3:7081235-7081254 | MS.gene051770:intron | 55.0% | |
| CCGCCAACCAGTCATTGCAA+AGG | + | chr7.3:7080886-7080905 | None:intergenic | 55.0% | |
| CTGCAGCCCTTTGCAATGAC+TGG | - | chr7.3:7080876-7080895 | MS.gene051770:CDS | 55.0% | |
| GCAGTTGCTGCATCAGGACT+AGG | - | chr7.3:7081218-7081237 | MS.gene051770:CDS | 55.0% | |
| GCATCAGGACTAGGCTATGC+TGG | - | chr7.3:7081227-7081246 | MS.gene051770:intron | 55.0% | |
| TACAGGTCGCACTTGTGCCA+GGG | - | chr7.3:7081662-7081681 | MS.gene051770:intron | 55.0% | |
| TCAGGACTAGGCTATGCTGG+TGG | - | chr7.3:7081230-7081249 | MS.gene051770:intron | 55.0% | |
| TCTACAGCTGCCTGTAGGGT+AGG | + | chr7.3:7081739-7081758 | None:intergenic | 55.0% | |
| CTACAGGTCGCACTTGTGCC+AGG | - | chr7.3:7081661-7081680 | MS.gene051770:intron | 60.0% | |
| TCGTCTCCAGATGCACTCCC+TGG | + | chr7.3:7081682-7081701 | None:intergenic | 60.0% | |
| ! | CTTGTGCCAGGGAGTGCATC+TGG | - | chr7.3:7081673-7081692 | MS.gene051770:intron | 60.0% |
| ! | GCAATGACTGGTTGGCGGCT+TGG | - | chr7.3:7080888-7080907 | MS.gene051770:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 7080616 | 7081807 | 7080616 | ID=MS.gene051770 |
| chr7.3 | mRNA | 7080616 | 7081807 | 7080616 | ID=MS.gene051770.t1;Parent=MS.gene051770 |
| chr7.3 | exon | 7081694 | 7081807 | 7081694 | ID=MS.gene051770.t1.exon1;Parent=MS.gene051770.t1 |
| chr7.3 | CDS | 7081694 | 7081807 | 7081694 | ID=cds.MS.gene051770.t1;Parent=MS.gene051770.t1 |
| chr7.3 | exon | 7081458 | 7081541 | 7081458 | ID=MS.gene051770.t1.exon2;Parent=MS.gene051770.t1 |
| chr7.3 | CDS | 7081458 | 7081541 | 7081458 | ID=cds.MS.gene051770.t1;Parent=MS.gene051770.t1 |
| chr7.3 | exon | 7081071 | 7081241 | 7081071 | ID=MS.gene051770.t1.exon3;Parent=MS.gene051770.t1 |
| chr7.3 | CDS | 7081071 | 7081241 | 7081071 | ID=cds.MS.gene051770.t1;Parent=MS.gene051770.t1 |
| chr7.3 | exon | 7080866 | 7080979 | 7080866 | ID=MS.gene051770.t1.exon4;Parent=MS.gene051770.t1 |
| chr7.3 | CDS | 7080866 | 7080979 | 7080866 | ID=cds.MS.gene051770.t1;Parent=MS.gene051770.t1 |
| chr7.3 | exon | 7080616 | 7080756 | 7080616 | ID=MS.gene051770.t1.exon5;Parent=MS.gene051770.t1 |
| chr7.3 | CDS | 7080616 | 7080756 | 7080616 | ID=cds.MS.gene051770.t1;Parent=MS.gene051770.t1 |
| Gene Sequence |
| Protein sequence |
