Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051772.t1 | XP_013450261.1 | 96.1 | 207 | 8 | 0 | 1 | 207 | 267 | 473 | 1.30E-104 | 389 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051772.t1 | E9L7A5 | 85.1 | 201 | 30 | 0 | 1 | 201 | 273 | 473 | 3.5e-93 | 342.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051772.t1 | A0A072U3J0 | 96.1 | 207 | 8 | 0 | 1 | 207 | 267 | 473 | 9.6e-105 | 389.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051770 | MS.gene051772 | 0.836045 | 1.18E-56 | -1.69E-46 |
| MS.gene051772 | MS.gene051773 | 0.802309 | 5.96E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051772.t1 | MTR_7g106830 | 96.135 | 207 | 8 | 0 | 1 | 207 | 267 | 473 | 6.97e-144 | 409 |
| MS.gene051772.t1 | MTR_8g091280 | 90.821 | 207 | 19 | 0 | 1 | 207 | 269 | 475 | 3.70e-135 | 387 |
| MS.gene051772.t1 | MTR_1807s0010 | 36.923 | 195 | 119 | 2 | 1 | 194 | 201 | 392 | 2.84e-33 | 123 |
| MS.gene051772.t1 | MTR_3g072670 | 27.094 | 203 | 129 | 6 | 1 | 198 | 255 | 443 | 5.16e-19 | 84.7 |
| MS.gene051772.t1 | MTR_4g011820 | 29.384 | 211 | 128 | 5 | 1 | 207 | 201 | 394 | 1.95e-18 | 82.8 |
| MS.gene051772.t1 | MTR_1g087760 | 30.841 | 214 | 118 | 7 | 2 | 198 | 194 | 394 | 2.02e-18 | 82.8 |
| MS.gene051772.t1 | MTR_3g072680 | 27.586 | 203 | 128 | 6 | 1 | 198 | 212 | 400 | 2.07e-18 | 82.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051772.t1 | AT2G22250 | 75.622 | 201 | 49 | 0 | 1 | 201 | 222 | 422 | 1.74e-108 | 318 |
| MS.gene051772.t1 | AT2G22250 | 75.622 | 201 | 49 | 0 | 1 | 201 | 269 | 469 | 8.78e-108 | 318 |
| MS.gene051772.t1 | AT2G22250 | 75.622 | 201 | 49 | 0 | 1 | 201 | 269 | 469 | 8.78e-108 | 318 |
| MS.gene051772.t1 | AT1G77670 | 28.571 | 203 | 126 | 6 | 1 | 198 | 248 | 436 | 1.01e-21 | 92.4 |
| MS.gene051772.t1 | AT1G80360 | 27.700 | 213 | 128 | 7 | 1 | 207 | 201 | 393 | 2.02e-15 | 74.3 |
| MS.gene051772.t1 | AT1G80360 | 27.700 | 213 | 128 | 7 | 1 | 207 | 201 | 393 | 2.02e-15 | 74.3 |
| MS.gene051772.t1 | AT1G80360 | 27.700 | 213 | 128 | 7 | 1 | 207 | 201 | 393 | 2.02e-15 | 74.3 |
| MS.gene051772.t1 | AT1G80360 | 27.700 | 213 | 128 | 7 | 1 | 207 | 201 | 393 | 2.02e-15 | 74.3 |
Find 49 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGATTTCAGCTCCTATTA+TGG | 0.261186 | 7.3:+7085195 | MS.gene051772:CDS |
| TTCAGCTTCTCTTCCATAAT+AGG | 0.283252 | 7.3:-7085208 | None:intergenic |
| TATTGAATTTAGTTTACTTC+GGG | 0.293177 | 7.3:+7084903 | MS.gene051772:intron |
| GGAAGAGAAGCTGAAGGTTT+TGG | 0.300530 | 7.3:+7085216 | MS.gene051772:CDS |
| TGGCGGCTTGGTTATATTGC+AGG | 0.312104 | 7.3:+7084633 | MS.gene051772:CDS |
| ATGCTGAAATAAAATGTTTC+GGG | 0.353525 | 7.3:-7084656 | None:intergenic |
| CTTGTGCCAGGGAGTGCATC+TGG | 0.364335 | 7.3:+7085406 | MS.gene051772:CDS |
| TGTCAATATCTGTTGCATAA+AGG | 0.366152 | 7.3:+7085264 | MS.gene051772:CDS |
| CATGCTGAAATAAAATGTTT+CGG | 0.387560 | 7.3:-7084657 | None:intergenic |
| CTACAGGTCGCACTTGTGCC+AGG | 0.411505 | 7.3:+7085394 | MS.gene051772:intron |
| ACAAGTGCAGCTGAGGTAAG+AGG | 0.413408 | 7.3:-7085514 | None:intergenic |
| CTGCAGCCCTTTGCAATGAC+TGG | 0.426334 | 7.3:+7084609 | MS.gene051772:intron |
| AAAGCTGCAGTTGCTGCATC+AGG | 0.433618 | 7.3:+7084945 | MS.gene051772:CDS |
| GCAATGACTGGTTGGCGGCT+TGG | 0.436164 | 7.3:+7084621 | MS.gene051772:CDS |
| GCAAAGCTAGTGTGTGTTGC+TGG | 0.444438 | 7.3:-7084388 | None:intergenic |
| TTATTGAATTTAGTTTACTT+CGG | 0.446135 | 7.3:+7084902 | MS.gene051772:intron |
| ATGTGGAAAGATTCAAAGTC+AGG | 0.449025 | 7.3:+7084677 | MS.gene051772:CDS |
| AACCTTAGAAAATCCATTCA+CGG | 0.462409 | 7.3:-7084443 | None:intergenic |
| GCAGTTGCTGCATCAGGACT+AGG | 0.466446 | 7.3:+7084951 | MS.gene051772:CDS |
| TCTCTCTACAGCTGCCTGTA+GGG | 0.480917 | 7.3:-7085473 | None:intergenic |
| GATAAAACGCTAACCGTGAA+TGG | 0.483375 | 7.3:+7084430 | MS.gene051772:CDS |
| GCATCAGGACTAGGCTATGC+TGG | 0.488598 | 7.3:+7084960 | MS.gene051772:CDS |
| AAGGATTCACACCTGTGGTT+CGG | 0.490759 | 7.3:-7085075 | None:intergenic |
| TAGAGAACGAAGGAATTACT+TGG | 0.497722 | 7.3:+7085016 | MS.gene051772:CDS |
| CGCCAACCAGTCATTGCAAA+GGG | 0.498469 | 7.3:-7084615 | None:intergenic |
| CCTTTGCAATGACTGGTTGG+CGG | 0.506213 | 7.3:+7084616 | MS.gene051772:CDS |
| TCGTCTCCAGATGCACTCCC+TGG | 0.507721 | 7.3:-7085412 | None:intergenic |
| ACTAGCTTTGCATCTTTACC+TGG | 0.509818 | 7.3:+7084400 | MS.gene051772:CDS |
| TGCATCTTTACCTGGAATGT+GGG | 0.520471 | 7.3:+7084408 | MS.gene051772:CDS |
| TTGCATCTTTACCTGGAATG+TGG | 0.521651 | 7.3:+7084407 | MS.gene051772:CDS |
| AGCATCTCTTCCTACCCTAC+AGG | 0.523160 | 7.3:+7085459 | MS.gene051772:CDS |
| AGCCCTTTGCAATGACTGGT+TGG | 0.531466 | 7.3:+7084613 | MS.gene051772:intron |
| CAAGTGCAGCTGAGGTAAGA+GGG | 0.556805 | 7.3:-7085513 | None:intergenic |
| ATATCTGTTGCATAAAGGCC+AGG | 0.563043 | 7.3:+7085269 | MS.gene051772:CDS |
| TACAGGTCGCACTTGTGCCA+GGG | 0.565282 | 7.3:+7085395 | MS.gene051772:intron |
| TTCTCTCTACAGCTGCCTGT+AGG | 0.565977 | 7.3:-7085474 | None:intergenic |
| TCTACAGCTGCCTGTAGGGT+AGG | 0.574697 | 7.3:-7085469 | None:intergenic |
| AGCTTTCTGAGATATACTAC+TGG | 0.576070 | 7.3:-7084928 | None:intergenic |
| ATTGAATTTAGTTTACTTCG+GGG | 0.577826 | 7.3:+7084904 | MS.gene051772:intron |
| CCGCCAACCAGTCATTGCAA+AGG | 0.598202 | 7.3:-7084616 | None:intergenic |
| TGTTGAAATATCCGAACCAC+AGG | 0.604423 | 7.3:+7085064 | MS.gene051772:CDS |
| TATTATGGAAGAGAAGCTGA+AGG | 0.610603 | 7.3:+7085210 | MS.gene051772:CDS |
| TTGAATTTAGTTTACTTCGG+GGG | 0.612433 | 7.3:+7084905 | MS.gene051772:intron |
| ACACTAGACAAGTGCAGCTG+AGG | 0.616702 | 7.3:-7085521 | None:intergenic |
| ACTAGGCTATGCTGGTGGAG+AGG | 0.620996 | 7.3:+7084968 | MS.gene051772:CDS |
| TTCTCTAAATGCTTTCACCA+TGG | 0.638071 | 7.3:-7085000 | None:intergenic |
| TGGAGAGGTTGTTTCTACCA+TGG | 0.638313 | 7.3:+7084983 | MS.gene051772:CDS |
| TCAGGACTAGGCTATGCTGG+TGG | 0.663983 | 7.3:+7084963 | MS.gene051772:CDS |
| TGAAAGCATTTAGAGAACGA+AGG | 0.676649 | 7.3:+7085006 | MS.gene051772:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTGATATTATATTATATG+CGG | + | chr7.3:7084859-7084878 | MS.gene051772:intron | 10.0% |
| !! | TATAAATTATGAAATAAACT+TGG | + | chr7.3:7084557-7084576 | MS.gene051772:intron | 10.0% |
| !!! | TTATTGAATTTAGTTTACTT+CGG | + | chr7.3:7084902-7084921 | MS.gene051772:intron | 15.0% |
| !! | AAAAAAAAATTAGAAGCCTT+AGG | - | chr7.3:7084588-7084607 | None:intergenic | 20.0% |
| !! | ACGAATATGGTTAAAATTAA+AGG | - | chr7.3:7084794-7084813 | None:intergenic | 20.0% |
| !! | CGAATATGGTTAAAATTAAA+GGG | - | chr7.3:7084793-7084812 | None:intergenic | 20.0% |
| !! | TCATAATTTATAACCATACT+AGG | - | chr7.3:7084549-7084568 | None:intergenic | 20.0% |
| !!! | TATTGAATTTAGTTTACTTC+GGG | + | chr7.3:7084903-7084922 | MS.gene051772:intron | 20.0% |
| ! | ATAAGATGAAGCTTAGAATA+CGG | - | chr7.3:7084729-7084748 | None:intergenic | 25.0% |
| ! | ATGCTGAAATAAAATGTTTC+GGG | - | chr7.3:7084659-7084678 | None:intergenic | 25.0% |
| ! | CATGCTGAAATAAAATGTTT+CGG | - | chr7.3:7084660-7084679 | None:intergenic | 25.0% |
| ! | TCACTCTTAAATAACGAATA+TGG | - | chr7.3:7084807-7084826 | None:intergenic | 25.0% |
| !! | ATTGAATTTAGTTTACTTCG+GGG | + | chr7.3:7084904-7084923 | MS.gene051772:intron | 25.0% |
| !!! | AAACATTTTATTTCAGCATG+TGG | + | chr7.3:7084660-7084679 | MS.gene051772:CDS | 25.0% |
| AACCTTAGAAAATCCATTCA+CGG | - | chr7.3:7084446-7084465 | None:intergenic | 30.0% | |
| AATAAACTTGGATGATCCTA+AGG | + | chr7.3:7084569-7084588 | MS.gene051772:intron | 30.0% | |
| GAGTATGCAAATAAACTAGA+AGG | - | chr7.3:7085097-7085116 | None:intergenic | 30.0% | |
| TGTCAATATCTGTTGCATAA+AGG | + | chr7.3:7085264-7085283 | MS.gene051772:CDS | 30.0% | |
| TTATGTTGCATATCCTAGTA+TGG | + | chr7.3:7084533-7084552 | MS.gene051772:intron | 30.0% | |
| ! | ATTGATTTCAGCTCCTATTA+TGG | + | chr7.3:7085195-7085214 | MS.gene051772:CDS | 30.0% |
| ! | TTGAATTTAGTTTACTTCGG+GGG | + | chr7.3:7084905-7084924 | MS.gene051772:intron | 30.0% |
| !! | AAAAGTTTTAGCGAAATCGA+TGG | + | chr7.3:7085041-7085060 | MS.gene051772:CDS | 30.0% |
| AGCGAACATAAAAGAGCAAA+AGG | - | chr7.3:7085119-7085138 | None:intergenic | 35.0% | |
| ATGTGGAAAGATTCAAAGTC+AGG | + | chr7.3:7084677-7084696 | MS.gene051772:CDS | 35.0% | |
| CAGAAAGAGAACATGACATA+TGG | - | chr7.3:7084472-7084491 | None:intergenic | 35.0% | |
| CATATGTCATGTTCTCTTTC+TGG | + | chr7.3:7084470-7084489 | MS.gene051772:intron | 35.0% | |
| GAAACTTCTGTGCATACATT+TGG | + | chr7.3:7084834-7084853 | MS.gene051772:intron | 35.0% | |
| TAACATTGTGTTTCGTCTAC+AGG | + | chr7.3:7085378-7085397 | MS.gene051772:intron | 35.0% | |
| TAGAGAACGAAGGAATTACT+TGG | + | chr7.3:7085016-7085035 | MS.gene051772:CDS | 35.0% | |
| TATTATGGAAGAGAAGCTGA+AGG | + | chr7.3:7085210-7085229 | MS.gene051772:CDS | 35.0% | |
| TGTCATTATATGAGCATACC+TGG | - | chr7.3:7085290-7085309 | None:intergenic | 35.0% | |
| TTCAGCTTCTCTTCCATAAT+AGG | - | chr7.3:7085211-7085230 | None:intergenic | 35.0% | |
| TTCTCTAAATGCTTTCACCA+TGG | - | chr7.3:7085003-7085022 | None:intergenic | 35.0% | |
| ! | ATACTTTATTTCCGCTTGCA+GGG | + | chr7.3:7085156-7085175 | MS.gene051772:intron | 35.0% |
| ! | TATACTTTATTTCCGCTTGC+AGG | + | chr7.3:7085155-7085174 | MS.gene051772:intron | 35.0% |
| !! | AACCGTGAATGGATTTTCTA+AGG | + | chr7.3:7084441-7084460 | MS.gene051772:CDS | 35.0% |
| !! | AGCTTTCTGAGATATACTAC+TGG | - | chr7.3:7084931-7084950 | None:intergenic | 35.0% |
| !! | TGAAAGCATTTAGAGAACGA+AGG | + | chr7.3:7085006-7085025 | MS.gene051772:CDS | 35.0% |
| !! | TGAAGGTTTTGGCAAAATTG+AGG | + | chr7.3:7085227-7085246 | MS.gene051772:CDS | 35.0% |
| AATAAAATGCTCCCTGCAAG+CGG | - | chr7.3:7085170-7085189 | None:intergenic | 40.0% | |
| ATATCTGTTGCATAAAGGCC+AGG | + | chr7.3:7085269-7085288 | MS.gene051772:CDS | 40.0% | |
| CTTGATTTCCTCTTGCTACA+AGG | + | chr7.3:7084749-7084768 | MS.gene051772:intron | 40.0% | |
| GATAAAACGCTAACCGTGAA+TGG | + | chr7.3:7084430-7084449 | MS.gene051772:CDS | 40.0% | |
| TGCATCTTTACCTGGAATGT+GGG | + | chr7.3:7084408-7084427 | MS.gene051772:CDS | 40.0% | |
| TGTTGAAATATCCGAACCAC+AGG | + | chr7.3:7085064-7085083 | MS.gene051772:CDS | 40.0% | |
| TTGCATCTTTACCTGGAATG+TGG | + | chr7.3:7084407-7084426 | MS.gene051772:CDS | 40.0% | |
| !! | ACTAGCTTTGCATCTTTACC+TGG | + | chr7.3:7084400-7084419 | MS.gene051772:CDS | 40.0% |
| AAGGATTCACACCTGTGGTT+CGG | - | chr7.3:7085078-7085097 | None:intergenic | 45.0% | |
| ACTAGAAGGATTCACACCTG+TGG | - | chr7.3:7085083-7085102 | None:intergenic | 45.0% | |
| CTTGCTACAAGGCATGCAAA+AGG | + | chr7.3:7084760-7084779 | MS.gene051772:intron | 45.0% | |
| TTTGCATGCCTTGTAGCAAG+AGG | - | chr7.3:7084760-7084779 | None:intergenic | 45.0% | |
| ! | AGCGTTTTATCCCACATTCC+AGG | - | chr7.3:7084421-7084440 | None:intergenic | 45.0% |
| ! | GGAAGAGAAGCTGAAGGTTT+TGG | + | chr7.3:7085216-7085235 | MS.gene051772:CDS | 45.0% |
| !! | TGGAGAGGTTGTTTCTACCA+TGG | + | chr7.3:7084983-7085002 | MS.gene051772:CDS | 45.0% |
| AAAGCTGCAGTTGCTGCATC+AGG | + | chr7.3:7084945-7084964 | MS.gene051772:CDS | 50.0% | |
| ACAAGTGCAGCTGAGGTAAG+AGG | - | chr7.3:7085517-7085536 | None:intergenic | 50.0% | |
| AGCATCTCTTCCTACCCTAC+AGG | + | chr7.3:7085459-7085478 | MS.gene051772:CDS | 50.0% | |
| AGCCCTTTGCAATGACTGGT+TGG | + | chr7.3:7084613-7084632 | MS.gene051772:intron | 50.0% | |
| CAAGTGCAGCTGAGGTAAGA+GGG | - | chr7.3:7085516-7085535 | None:intergenic | 50.0% | |
| CGCCAACCAGTCATTGCAAA+GGG | - | chr7.3:7084618-7084637 | None:intergenic | 50.0% | |
| GCAAAGCTAGTGTGTGTTGC+TGG | - | chr7.3:7084391-7084410 | None:intergenic | 50.0% | |
| TACCTCAGCTGCACTTGTCT+AGG | + | chr7.3:7085519-7085538 | MS.gene051772:CDS | 50.0% | |
| TCTCTCTACAGCTGCCTGTA+GGG | - | chr7.3:7085476-7085495 | None:intergenic | 50.0% | |
| TTCTCTCTACAGCTGCCTGT+AGG | - | chr7.3:7085477-7085496 | None:intergenic | 50.0% | |
| ! | CCTTTGCAATGACTGGTTGG+CGG | + | chr7.3:7084616-7084635 | MS.gene051772:CDS | 50.0% |
| !! | TGGCGGCTTGGTTATATTGC+AGG | + | chr7.3:7084633-7084652 | MS.gene051772:CDS | 50.0% |
| ACTAGGCTATGCTGGTGGAG+AGG | + | chr7.3:7084968-7084987 | MS.gene051772:CDS | 55.0% | |
| CCGCCAACCAGTCATTGCAA+AGG | - | chr7.3:7084619-7084638 | None:intergenic | 55.0% | |
| CTGCAGCCCTTTGCAATGAC+TGG | + | chr7.3:7084609-7084628 | MS.gene051772:intron | 55.0% | |
| GCAGTTGCTGCATCAGGACT+AGG | + | chr7.3:7084951-7084970 | MS.gene051772:CDS | 55.0% | |
| GCATCAGGACTAGGCTATGC+TGG | + | chr7.3:7084960-7084979 | MS.gene051772:CDS | 55.0% | |
| TACAGGTCGCACTTGTGCCA+GGG | + | chr7.3:7085395-7085414 | MS.gene051772:intron | 55.0% | |
| TCAGGACTAGGCTATGCTGG+TGG | + | chr7.3:7084963-7084982 | MS.gene051772:CDS | 55.0% | |
| TCTACAGCTGCCTGTAGGGT+AGG | - | chr7.3:7085472-7085491 | None:intergenic | 55.0% | |
| CTACAGGTCGCACTTGTGCC+AGG | + | chr7.3:7085394-7085413 | MS.gene051772:intron | 60.0% | |
| TCGTCTCCAGATGCACTCCC+TGG | - | chr7.3:7085415-7085434 | None:intergenic | 60.0% | |
| ! | CTTGTGCCAGGGAGTGCATC+TGG | + | chr7.3:7085406-7085425 | MS.gene051772:CDS | 60.0% |
| ! | GCAATGACTGGTTGGCGGCT+TGG | + | chr7.3:7084621-7084640 | MS.gene051772:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 7084349 | 7085540 | 7084349 | ID=MS.gene051772 |
| chr7.3 | mRNA | 7084349 | 7085540 | 7084349 | ID=MS.gene051772.t1;Parent=MS.gene051772 |
| chr7.3 | exon | 7084349 | 7084462 | 7084349 | ID=MS.gene051772.t1.exon1;Parent=MS.gene051772.t1 |
| chr7.3 | CDS | 7084349 | 7084462 | 7084349 | ID=cds.MS.gene051772.t1;Parent=MS.gene051772.t1 |
| chr7.3 | exon | 7084615 | 7084698 | 7084615 | ID=MS.gene051772.t1.exon2;Parent=MS.gene051772.t1 |
| chr7.3 | CDS | 7084615 | 7084698 | 7084615 | ID=cds.MS.gene051772.t1;Parent=MS.gene051772.t1 |
| chr7.3 | exon | 7084915 | 7085085 | 7084915 | ID=MS.gene051772.t1.exon3;Parent=MS.gene051772.t1 |
| chr7.3 | CDS | 7084915 | 7085085 | 7084915 | ID=cds.MS.gene051772.t1;Parent=MS.gene051772.t1 |
| chr7.3 | exon | 7085177 | 7085290 | 7085177 | ID=MS.gene051772.t1.exon4;Parent=MS.gene051772.t1 |
| chr7.3 | CDS | 7085177 | 7085290 | 7085177 | ID=cds.MS.gene051772.t1;Parent=MS.gene051772.t1 |
| chr7.3 | exon | 7085400 | 7085540 | 7085400 | ID=MS.gene051772.t1.exon5;Parent=MS.gene051772.t1 |
| chr7.3 | CDS | 7085400 | 7085540 | 7085400 | ID=cds.MS.gene051772.t1;Parent=MS.gene051772.t1 |
| Gene Sequence |
| Protein sequence |
