Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051779.t1 | KEH32425.1 | 70.4 | 108 | 31 | 1 | 1 | 108 | 67 | 173 | 6.20E-30 | 140.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051779.t1 | A2XD79 | 47.0 | 83 | 41 | 2 | 32 | 111 | 96 | 178 | 3.6e-10 | 65.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051779.t1 | A0A072V2U3 | 70.4 | 108 | 31 | 1 | 1 | 108 | 67 | 173 | 4.5e-30 | 140.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene051779.t1 | TF | Tify |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051779 | MS.gene24788 | 0.836416 | 9.48E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051779.t1 | MTR_4g124950 | 70.175 | 114 | 22 | 2 | 1 | 106 | 1 | 110 | 8.13e-47 | 147 |
| MS.gene051779.t1 | MTR_4g124960 | 70.370 | 108 | 31 | 1 | 1 | 108 | 67 | 173 | 2.33e-45 | 145 |
| MS.gene051779.t1 | MTR_4g124960 | 70.755 | 106 | 30 | 1 | 1 | 106 | 67 | 171 | 1.70e-44 | 144 |
| MS.gene051779.t1 | MTR_2g042900 | 38.608 | 158 | 44 | 6 | 1 | 106 | 72 | 228 | 3.86e-22 | 87.8 |
| MS.gene051779.t1 | MTR_2g042900 | 43.617 | 94 | 33 | 3 | 1 | 74 | 72 | 165 | 3.15e-17 | 74.7 |
| MS.gene051779.t1 | MTR_5g013515 | 36.190 | 105 | 44 | 3 | 25 | 106 | 4 | 108 | 5.02e-14 | 64.3 |
| MS.gene051779.t1 | MTR_5g013530 | 40.625 | 96 | 40 | 3 | 30 | 108 | 17 | 112 | 1.22e-13 | 63.5 |
| MS.gene051779.t1 | MTR_5g013530 | 37.692 | 130 | 53 | 5 | 7 | 108 | 5 | 134 | 1.61e-13 | 63.5 |
| MS.gene051779.t1 | MTR_5g013520 | 37.234 | 94 | 42 | 2 | 30 | 106 | 10 | 103 | 7.89e-12 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051779.t1 | AT1G19180 | 32.609 | 138 | 59 | 3 | 2 | 105 | 17 | 154 | 4.31e-11 | 57.4 |
| MS.gene051779.t1 | AT1G19180 | 36.190 | 105 | 41 | 2 | 30 | 108 | 119 | 223 | 8.14e-11 | 57.4 |
| MS.gene051779.t1 | AT1G19180 | 36.190 | 105 | 41 | 2 | 30 | 108 | 119 | 223 | 8.14e-11 | 57.4 |
Find 21 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTCACTTCTTCGATTCA+TGG | 0.237608 | 8.2:+7708524 | MS.gene051779:CDS |
| AATGTCAACTGTGCAGTTTC+CGG | 0.323565 | 8.2:-7708230 | None:intergenic |
| TCTAGTAACACAAGTGGCTT+TGG | 0.360249 | 8.2:-7709130 | None:intergenic |
| CAACCACCTCCTACACTAAC+TGG | 0.374106 | 8.2:+7708368 | MS.gene051779:CDS |
| TTATTTCTTTAAAGAAGATA+TGG | 0.376656 | 8.2:+7708488 | MS.gene051779:intron |
| TAATGGAGAAGTTATTGTAT+TGG | 0.400656 | 8.2:+7708259 | MS.gene051779:CDS |
| TAACTCAATGGTTAACAAAC+CGG | 0.425259 | 8.2:+7708211 | MS.gene051779:CDS |
| GGTGGTTGAAGACTCATATC+AGG | 0.439597 | 8.2:-7708353 | None:intergenic |
| AGCTCCTCAACTTTGTCAGC+AGG | 0.455494 | 8.2:-7708290 | None:intergenic |
| TTACAGAACTAATAACTCAA+TGG | 0.476118 | 8.2:+7708199 | MS.gene051779:intron |
| TTCTTCGATTCATGGAGAAA+AGG | 0.478429 | 8.2:+7708532 | MS.gene051779:CDS |
| CAATTACCAGTTAGTGTAGG+AGG | 0.522487 | 8.2:-7708374 | None:intergenic |
| CTGTGGATTCTTTGTCTCCA+AGG | 0.531713 | 8.2:+7708055 | MS.gene051779:CDS |
| TCATGGAGAAAAGGAAAGCC+AGG | 0.536399 | 8.2:+7708541 | MS.gene051779:CDS |
| CATTTGCAAGCACTCAAACT+CGG | 0.560353 | 8.2:+7708318 | MS.gene051779:CDS |
| CTCATATCAGGAATAACTGA+AGG | 0.598557 | 8.2:-7708341 | None:intergenic |
| TCAATTTCTAGTAACACAAG+TGG | 0.602083 | 8.2:-7709136 | None:intergenic |
| GTTTGTGGGAAATAAGTCCA+TGG | 0.603000 | 8.2:-7708010 | None:intergenic |
| TTACCAGTTAGTGTAGGAGG+TGG | 0.608915 | 8.2:-7708371 | None:intergenic |
| GAGCAATTACCAGTTAGTGT+AGG | 0.610011 | 8.2:-7708377 | None:intergenic |
| CTTGGAGACAAAGAATCCAC+AGG | 0.648213 | 8.2:-7708054 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATATTTTGAAACAAATAA+AGG | + | chr8.2:7708412-7708431 | MS.gene051779:intron | 10.0% |
| !! | AAGAAATAAAATTTATGATC+AGG | - | chr8.2:7708477-7708496 | None:intergenic | 15.0% |
| !!! | AAGGAAATATTTTTTGTATT+AGG | + | chr8.2:7708431-7708450 | MS.gene051779:intron | 15.0% |
| !!! | AGGAAATATTTTTTGTATTA+GGG | + | chr8.2:7708432-7708451 | MS.gene051779:intron | 15.0% |
| !!! | ATTATGAATATTTTGATTAC+CGG | - | chr8.2:7708837-7708856 | None:intergenic | 15.0% |
| !!! | CAATCAACTAATATTTTTTT+GGG | + | chr8.2:7708720-7708739 | MS.gene051779:intron | 15.0% |
| !!! | TAAATTCAAGTTTTTATTCA+AGG | + | chr8.2:7708582-7708601 | MS.gene051779:intron | 15.0% |
| !!! | TCAATCAACTAATATTTTTT+TGG | + | chr8.2:7708719-7708738 | MS.gene051779:intron | 15.0% |
| !!! | TTATTTCTTTAAAGAAGATA+TGG | + | chr8.2:7708488-7708507 | MS.gene051779:intron | 15.0% |
| ! | TAATGGAGAAGTTATTGTAT+TGG | + | chr8.2:7708259-7708278 | MS.gene051779:CDS | 25.0% |
| ! | TTACAGAACTAATAACTCAA+TGG | + | chr8.2:7708199-7708218 | MS.gene051779:intron | 25.0% |
| ! | TTGTTTCAACATTTCAATCA+AGG | - | chr8.2:7708697-7708716 | None:intergenic | 25.0% |
| !!! | CAGTTGACATTGTTTTATAA+TGG | + | chr8.2:7708242-7708261 | MS.gene051779:CDS | 25.0% |
| !!! | GTAAAATGTGATTTTTGAGA+TGG | - | chr8.2:7709070-7709089 | None:intergenic | 25.0% |
| !!! | TTGAATTTAATTTGTCTGAG+AGG | - | chr8.2:7708571-7708590 | None:intergenic | 25.0% |
| GTTGTCGTAACTTTGTAATT+TGG | + | chr8.2:7708964-7708983 | MS.gene051779:intron | 30.0% | |
| TAACTCAATGGTTAACAAAC+CGG | + | chr8.2:7708211-7708230 | MS.gene051779:CDS | 30.0% | |
| !! | AGTAGTTGATGATATGCATA+AGG | - | chr8.2:7709037-7709056 | None:intergenic | 30.0% |
| !! | TCAATTTCTAGTAACACAAG+TGG | - | chr8.2:7709139-7709158 | None:intergenic | 30.0% |
| CTCATATCAGGAATAACTGA+AGG | - | chr8.2:7708344-7708363 | None:intergenic | 35.0% | |
| TAGAATTGGATGGAATCAGT+TGG | - | chr8.2:7709096-7709115 | None:intergenic | 35.0% | |
| TTCTTCGATTCATGGAGAAA+AGG | + | chr8.2:7708532-7708551 | MS.gene051779:CDS | 35.0% | |
| ! | AACTGATTCCATCCAATTCT+AGG | + | chr8.2:7709095-7709114 | MS.gene051779:intron | 35.0% |
| ! | AGAAGCACAAAAACATACCT+TGG | - | chr8.2:7708075-7708094 | None:intergenic | 35.0% |
| !!! | ACTTTTTTGCACACAAGCAA+TGG | - | chr8.2:7708138-7708157 | None:intergenic | 35.0% |
| !!! | CAACTTTCGTGTTGTTTTCA+CGG | - | chr8.2:7708948-7708967 | None:intergenic | 35.0% |
| !!! | CTTTTTTGCACACAAGCAAT+GGG | - | chr8.2:7708137-7708156 | None:intergenic | 35.0% |
| !!! | TTTTTTGCACACAAGCAATG+GGG | - | chr8.2:7708136-7708155 | None:intergenic | 35.0% |
| !!! | TTTTTTGGTGTCACGTTTGT+GGG | - | chr8.2:7708027-7708046 | None:intergenic | 35.0% |
| AATGTCAACTGTGCAGTTTC+CGG | - | chr8.2:7708233-7708252 | None:intergenic | 40.0% | |
| ACCAAAAAACGTTACACCTG+TGG | + | chr8.2:7708038-7708057 | MS.gene051779:CDS | 40.0% | |
| AGCTTCACTTCTTCGATTCA+TGG | + | chr8.2:7708524-7708543 | MS.gene051779:CDS | 40.0% | |
| ATAGACAGAGACATGGAGAA+GGG | - | chr8.2:7708674-7708693 | None:intergenic | 40.0% | |
| ATCAAGGATAGACAGAGACA+TGG | - | chr8.2:7708681-7708700 | None:intergenic | 40.0% | |
| CAAGCAAAATCAAGGACTTC+CGG | + | chr8.2:7708815-7708834 | MS.gene051779:intron | 40.0% | |
| CAATTACCAGTTAGTGTAGG+AGG | - | chr8.2:7708377-7708396 | None:intergenic | 40.0% | |
| CACTATTTATGTTCCTCTCG+TGG | - | chr8.2:7708883-7708902 | None:intergenic | 40.0% | |
| CATTTGCAAGCACTCAAACT+CGG | + | chr8.2:7708318-7708337 | MS.gene051779:CDS | 40.0% | |
| GAGCAATTACCAGTTAGTGT+AGG | - | chr8.2:7708380-7708399 | None:intergenic | 40.0% | |
| GCAATGGGGCAAAACAAAAA+TGG | - | chr8.2:7708122-7708141 | None:intergenic | 40.0% | |
| TCTAGTAACACAAGTGGCTT+TGG | - | chr8.2:7709133-7709152 | None:intergenic | 40.0% | |
| TTGTGCTCATGCTCTTACAA+GGG | - | chr8.2:7708909-7708928 | None:intergenic | 40.0% | |
| ! | TTTTCCTGCTGACAAAGTTG+AGG | + | chr8.2:7708286-7708305 | MS.gene051779:CDS | 40.0% |
| !!! | GTTTTTTGGTGTCACGTTTG+TGG | - | chr8.2:7708028-7708047 | None:intergenic | 40.0% |
| !!! | TCCACAGGTGTAACGTTTTT+TGG | - | chr8.2:7708042-7708061 | None:intergenic | 40.0% |
| !!! | TTCGTGTTGTTTTCACGGTA+CGG | - | chr8.2:7708943-7708962 | None:intergenic | 40.0% |
| CTCTTCAGCAAGCCTAGAAT+TGG | - | chr8.2:7709110-7709129 | None:intergenic | 45.0% | |
| CTGTGGATTCTTTGTCTCCA+AGG | + | chr8.2:7708055-7708074 | MS.gene051779:CDS | 45.0% | |
| CTTCGACGCAAGCAAAATCA+AGG | + | chr8.2:7708807-7708826 | MS.gene051779:intron | 45.0% | |
| CTTGGAGACAAAGAATCCAC+AGG | - | chr8.2:7708057-7708076 | None:intergenic | 45.0% | |
| GATAGACAGAGACATGGAGA+AGG | - | chr8.2:7708675-7708694 | None:intergenic | 45.0% | |
| GGTGGTTGAAGACTCATATC+AGG | - | chr8.2:7708356-7708375 | None:intergenic | 45.0% | |
| GTTGTGCTCATGCTCTTACA+AGG | - | chr8.2:7708910-7708929 | None:intergenic | 45.0% | |
| TCAGCAAGCCTAGAATTGGA+TGG | - | chr8.2:7709106-7709125 | None:intergenic | 45.0% | |
| TCATAGTCAAGAACCACGAG+AGG | + | chr8.2:7708867-7708886 | MS.gene051779:intron | 45.0% | |
| TCATGGAGAAAAGGAAAGCC+AGG | + | chr8.2:7708541-7708560 | MS.gene051779:CDS | 45.0% | |
| TTACCAGTTAGTGTAGGAGG+TGG | - | chr8.2:7708374-7708393 | None:intergenic | 45.0% | |
| ! | ATTTGTCTGAGAGGCATACC+TGG | - | chr8.2:7708562-7708581 | None:intergenic | 45.0% |
| ! | CTTGATTTTGCTTGCGTCGA+AGG | - | chr8.2:7708809-7708828 | None:intergenic | 45.0% |
| AGCTCCTCAACTTTGTCAGC+AGG | - | chr8.2:7708293-7708312 | None:intergenic | 50.0% | |
| CAACCACCTCCTACACTAAC+TGG | + | chr8.2:7708368-7708387 | MS.gene051779:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 7708012 | 7709165 | 7708012 | ID=MS.gene051779 |
| chr8.2 | mRNA | 7708012 | 7709165 | 7708012 | ID=MS.gene051779.t1;Parent=MS.gene051779 |
| chr8.2 | exon | 7708012 | 7708076 | 7708012 | ID=MS.gene051779.t1.exon1;Parent=MS.gene051779.t1 |
| chr8.2 | CDS | 7708012 | 7708076 | 7708012 | ID=cds.MS.gene051779.t1;Parent=MS.gene051779.t1 |
| chr8.2 | exon | 7708205 | 7708389 | 7708205 | ID=MS.gene051779.t1.exon2;Parent=MS.gene051779.t1 |
| chr8.2 | CDS | 7708205 | 7708389 | 7708205 | ID=cds.MS.gene051779.t1;Parent=MS.gene051779.t1 |
| chr8.2 | exon | 7708502 | 7708562 | 7708502 | ID=MS.gene051779.t1.exon3;Parent=MS.gene051779.t1 |
| chr8.2 | CDS | 7708502 | 7708562 | 7708502 | ID=cds.MS.gene051779.t1;Parent=MS.gene051779.t1 |
| chr8.2 | exon | 7709117 | 7709165 | 7709117 | ID=MS.gene051779.t1.exon4;Parent=MS.gene051779.t1 |
| chr8.2 | CDS | 7709117 | 7709165 | 7709117 | ID=cds.MS.gene051779.t1;Parent=MS.gene051779.t1 |
| Gene Sequence |
| Protein sequence |
