Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24788.t1 | KEH32425.1 | 70.4 | 108 | 31 | 1 | 1 | 108 | 67 | 173 | 6.20E-30 | 140.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24788.t1 | A2XD79 | 47.0 | 83 | 41 | 2 | 32 | 111 | 96 | 178 | 3.6e-10 | 65.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24788.t1 | A0A072V2U3 | 70.4 | 108 | 31 | 1 | 1 | 108 | 67 | 173 | 4.5e-30 | 140.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene24788.t1 | TF | Tify |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051779 | MS.gene24788 | 0.836416 | 9.48E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24788.t1 | MTR_4g124950 | 70.175 | 114 | 22 | 2 | 1 | 106 | 1 | 110 | 8.13e-47 | 147 |
| MS.gene24788.t1 | MTR_4g124960 | 70.370 | 108 | 31 | 1 | 1 | 108 | 67 | 173 | 2.33e-45 | 145 |
| MS.gene24788.t1 | MTR_4g124960 | 70.755 | 106 | 30 | 1 | 1 | 106 | 67 | 171 | 1.70e-44 | 144 |
| MS.gene24788.t1 | MTR_2g042900 | 38.608 | 158 | 44 | 6 | 1 | 106 | 72 | 228 | 3.86e-22 | 87.8 |
| MS.gene24788.t1 | MTR_2g042900 | 43.617 | 94 | 33 | 3 | 1 | 74 | 72 | 165 | 3.15e-17 | 74.7 |
| MS.gene24788.t1 | MTR_5g013515 | 36.190 | 105 | 44 | 3 | 25 | 106 | 4 | 108 | 5.02e-14 | 64.3 |
| MS.gene24788.t1 | MTR_5g013530 | 40.625 | 96 | 40 | 3 | 30 | 108 | 17 | 112 | 1.22e-13 | 63.5 |
| MS.gene24788.t1 | MTR_5g013530 | 37.692 | 130 | 53 | 5 | 7 | 108 | 5 | 134 | 1.61e-13 | 63.5 |
| MS.gene24788.t1 | MTR_5g013520 | 37.234 | 94 | 42 | 2 | 30 | 106 | 10 | 103 | 7.89e-12 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24788.t1 | AT1G19180 | 32.609 | 138 | 59 | 3 | 2 | 105 | 17 | 154 | 4.31e-11 | 57.4 |
| MS.gene24788.t1 | AT1G19180 | 36.190 | 105 | 41 | 2 | 30 | 108 | 119 | 223 | 8.14e-11 | 57.4 |
| MS.gene24788.t1 | AT1G19180 | 36.190 | 105 | 41 | 2 | 30 | 108 | 119 | 223 | 8.14e-11 | 57.4 |
Find 21 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTCACTTCTTCGATTCA+TGG | 0.237608 | 8.2:+7740089 | MS.gene24788:CDS |
| AATGTCAACTGTGCAGTTTC+CGG | 0.323565 | 8.2:-7739795 | None:intergenic |
| TCTAGTAACACAAGTGGCTT+TGG | 0.360249 | 8.2:-7740695 | None:intergenic |
| CAACCACCTCCTACACTAAC+TGG | 0.374106 | 8.2:+7739933 | MS.gene24788:CDS |
| TTATTTCTTTAAAGAAGATA+TGG | 0.376656 | 8.2:+7740053 | MS.gene24788:intron |
| TAATGGAGAAGTTATTGTAT+TGG | 0.400656 | 8.2:+7739824 | MS.gene24788:CDS |
| TAACTCAATGGTTAACAAAC+CGG | 0.425259 | 8.2:+7739776 | MS.gene24788:CDS |
| GGTGGTTGAAGACTCATATC+AGG | 0.439597 | 8.2:-7739918 | None:intergenic |
| AGCTCCTCAACTTTGTCAGC+AGG | 0.455494 | 8.2:-7739855 | None:intergenic |
| TTACAGAACTAATAACTCAA+TGG | 0.476118 | 8.2:+7739764 | MS.gene24788:intron |
| TTCTTCGATTCATGGAGAAA+AGG | 0.478429 | 8.2:+7740097 | MS.gene24788:CDS |
| CAATTACCAGTTAGTGTAGG+AGG | 0.522487 | 8.2:-7739939 | None:intergenic |
| CTGTGGATTCTTTGTCTCCA+AGG | 0.531713 | 8.2:+7739620 | MS.gene24788:CDS |
| TCATGGAGAAAAGGAAAGCC+AGG | 0.536399 | 8.2:+7740106 | MS.gene24788:CDS |
| CATTTGCAAGCACTCAAACT+CGG | 0.560353 | 8.2:+7739883 | MS.gene24788:CDS |
| CTCATATCAGGAATAACTGA+AGG | 0.598557 | 8.2:-7739906 | None:intergenic |
| TCAATTTCTAGTAACACAAG+TGG | 0.602083 | 8.2:-7740701 | None:intergenic |
| GTTTGTGGGAAATAAGTCCA+TGG | 0.603000 | 8.2:-7739575 | None:intergenic |
| TTACCAGTTAGTGTAGGAGG+TGG | 0.608915 | 8.2:-7739936 | None:intergenic |
| GAGCAATTACCAGTTAGTGT+AGG | 0.610011 | 8.2:-7739942 | None:intergenic |
| CTTGGAGACAAAGAATCCAC+AGG | 0.648213 | 8.2:-7739619 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATATTTTGAAACAAATAA+AGG | + | chr8.2:7739977-7739996 | MS.gene24788:intron | 10.0% |
| !! | AAGAAATAAAATTTATGATC+AGG | - | chr8.2:7740042-7740061 | None:intergenic | 15.0% |
| !!! | AAGGAAATATTTTTTGTATT+AGG | + | chr8.2:7739996-7740015 | MS.gene24788:intron | 15.0% |
| !!! | AGGAAATATTTTTTGTATTA+GGG | + | chr8.2:7739997-7740016 | MS.gene24788:intron | 15.0% |
| !!! | ATTATGAATATTTTGATTAC+CGG | - | chr8.2:7740402-7740421 | None:intergenic | 15.0% |
| !!! | CAATCAACTAATATTTTTTT+GGG | + | chr8.2:7740285-7740304 | MS.gene24788:intron | 15.0% |
| !!! | TAAATTCAAGTTTTTATTCA+AGG | + | chr8.2:7740147-7740166 | MS.gene24788:intron | 15.0% |
| !!! | TCAATCAACTAATATTTTTT+TGG | + | chr8.2:7740284-7740303 | MS.gene24788:intron | 15.0% |
| !!! | TTATTTCTTTAAAGAAGATA+TGG | + | chr8.2:7740053-7740072 | MS.gene24788:intron | 15.0% |
| ! | TAATGGAGAAGTTATTGTAT+TGG | + | chr8.2:7739824-7739843 | MS.gene24788:CDS | 25.0% |
| ! | TTACAGAACTAATAACTCAA+TGG | + | chr8.2:7739764-7739783 | MS.gene24788:intron | 25.0% |
| ! | TTGTTTCAACATTTCAATCA+AGG | - | chr8.2:7740262-7740281 | None:intergenic | 25.0% |
| !!! | CAGTTGACATTGTTTTATAA+TGG | + | chr8.2:7739807-7739826 | MS.gene24788:CDS | 25.0% |
| !!! | GTAAAATGTGATTTTTGAGA+TGG | - | chr8.2:7740635-7740654 | None:intergenic | 25.0% |
| !!! | TTGAATTTAATTTGTCTGAG+AGG | - | chr8.2:7740136-7740155 | None:intergenic | 25.0% |
| GTTGTCGTAACTTTGTAATT+TGG | + | chr8.2:7740529-7740548 | MS.gene24788:intron | 30.0% | |
| TAACTCAATGGTTAACAAAC+CGG | + | chr8.2:7739776-7739795 | MS.gene24788:CDS | 30.0% | |
| !! | AGTAGTTGATGATATGCATA+AGG | - | chr8.2:7740602-7740621 | None:intergenic | 30.0% |
| !! | TCAATTTCTAGTAACACAAG+TGG | - | chr8.2:7740704-7740723 | None:intergenic | 30.0% |
| CTCATATCAGGAATAACTGA+AGG | - | chr8.2:7739909-7739928 | None:intergenic | 35.0% | |
| TAGAATTGGATGGAATCAGT+TGG | - | chr8.2:7740661-7740680 | None:intergenic | 35.0% | |
| TTCTTCGATTCATGGAGAAA+AGG | + | chr8.2:7740097-7740116 | MS.gene24788:CDS | 35.0% | |
| ! | AACTGATTCCATCCAATTCT+AGG | + | chr8.2:7740660-7740679 | MS.gene24788:intron | 35.0% |
| ! | AGAAGCACAAAAACATACCT+TGG | - | chr8.2:7739640-7739659 | None:intergenic | 35.0% |
| !!! | ACTTTTTTGCACACAAGCAA+TGG | - | chr8.2:7739703-7739722 | None:intergenic | 35.0% |
| !!! | CAACTTTCGTGTTGTTTTCA+CGG | - | chr8.2:7740513-7740532 | None:intergenic | 35.0% |
| !!! | CTTTTTTGCACACAAGCAAT+GGG | - | chr8.2:7739702-7739721 | None:intergenic | 35.0% |
| !!! | TTTTTTGCACACAAGCAATG+GGG | - | chr8.2:7739701-7739720 | None:intergenic | 35.0% |
| !!! | TTTTTTGGTGTCACGTTTGT+GGG | - | chr8.2:7739592-7739611 | None:intergenic | 35.0% |
| AATGTCAACTGTGCAGTTTC+CGG | - | chr8.2:7739798-7739817 | None:intergenic | 40.0% | |
| ACCAAAAAACGTTACACCTG+TGG | + | chr8.2:7739603-7739622 | MS.gene24788:CDS | 40.0% | |
| AGCTTCACTTCTTCGATTCA+TGG | + | chr8.2:7740089-7740108 | MS.gene24788:CDS | 40.0% | |
| ATAGACAGAGACATGGAGAA+GGG | - | chr8.2:7740239-7740258 | None:intergenic | 40.0% | |
| ATCAAGGATAGACAGAGACA+TGG | - | chr8.2:7740246-7740265 | None:intergenic | 40.0% | |
| CAAGCAAAATCAAGGACTTC+CGG | + | chr8.2:7740380-7740399 | MS.gene24788:intron | 40.0% | |
| CAATTACCAGTTAGTGTAGG+AGG | - | chr8.2:7739942-7739961 | None:intergenic | 40.0% | |
| CACTATTTATGTTCCTCTCG+TGG | - | chr8.2:7740448-7740467 | None:intergenic | 40.0% | |
| CATTTGCAAGCACTCAAACT+CGG | + | chr8.2:7739883-7739902 | MS.gene24788:CDS | 40.0% | |
| GAGCAATTACCAGTTAGTGT+AGG | - | chr8.2:7739945-7739964 | None:intergenic | 40.0% | |
| GCAATGGGGCAAAACAAAAA+TGG | - | chr8.2:7739687-7739706 | None:intergenic | 40.0% | |
| TCTAGTAACACAAGTGGCTT+TGG | - | chr8.2:7740698-7740717 | None:intergenic | 40.0% | |
| TTGTGCTCATGCTCTTACAA+GGG | - | chr8.2:7740474-7740493 | None:intergenic | 40.0% | |
| ! | TTTTCCTGCTGACAAAGTTG+AGG | + | chr8.2:7739851-7739870 | MS.gene24788:CDS | 40.0% |
| !!! | GTTTTTTGGTGTCACGTTTG+TGG | - | chr8.2:7739593-7739612 | None:intergenic | 40.0% |
| !!! | TCCACAGGTGTAACGTTTTT+TGG | - | chr8.2:7739607-7739626 | None:intergenic | 40.0% |
| !!! | TTCGTGTTGTTTTCACGGTA+CGG | - | chr8.2:7740508-7740527 | None:intergenic | 40.0% |
| CTCTTCAGCAAGCCTAGAAT+TGG | - | chr8.2:7740675-7740694 | None:intergenic | 45.0% | |
| CTGTGGATTCTTTGTCTCCA+AGG | + | chr8.2:7739620-7739639 | MS.gene24788:CDS | 45.0% | |
| CTTCGACGCAAGCAAAATCA+AGG | + | chr8.2:7740372-7740391 | MS.gene24788:intron | 45.0% | |
| CTTGGAGACAAAGAATCCAC+AGG | - | chr8.2:7739622-7739641 | None:intergenic | 45.0% | |
| GATAGACAGAGACATGGAGA+AGG | - | chr8.2:7740240-7740259 | None:intergenic | 45.0% | |
| GGTGGTTGAAGACTCATATC+AGG | - | chr8.2:7739921-7739940 | None:intergenic | 45.0% | |
| GTTGTGCTCATGCTCTTACA+AGG | - | chr8.2:7740475-7740494 | None:intergenic | 45.0% | |
| TCAGCAAGCCTAGAATTGGA+TGG | - | chr8.2:7740671-7740690 | None:intergenic | 45.0% | |
| TCATAGTCAAGAACCACGAG+AGG | + | chr8.2:7740432-7740451 | MS.gene24788:intron | 45.0% | |
| TCATGGAGAAAAGGAAAGCC+AGG | + | chr8.2:7740106-7740125 | MS.gene24788:CDS | 45.0% | |
| TTACCAGTTAGTGTAGGAGG+TGG | - | chr8.2:7739939-7739958 | None:intergenic | 45.0% | |
| ! | ATTTGTCTGAGAGGCATACC+TGG | - | chr8.2:7740127-7740146 | None:intergenic | 45.0% |
| ! | CTTGATTTTGCTTGCGTCGA+AGG | - | chr8.2:7740374-7740393 | None:intergenic | 45.0% |
| AGCTCCTCAACTTTGTCAGC+AGG | - | chr8.2:7739858-7739877 | None:intergenic | 50.0% | |
| CAACCACCTCCTACACTAAC+TGG | + | chr8.2:7739933-7739952 | MS.gene24788:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 7739577 | 7740730 | 7739577 | ID=MS.gene24788 |
| chr8.2 | mRNA | 7739577 | 7740730 | 7739577 | ID=MS.gene24788.t1;Parent=MS.gene24788 |
| chr8.2 | exon | 7739577 | 7739641 | 7739577 | ID=MS.gene24788.t1.exon1;Parent=MS.gene24788.t1 |
| chr8.2 | CDS | 7739577 | 7739641 | 7739577 | ID=cds.MS.gene24788.t1;Parent=MS.gene24788.t1 |
| chr8.2 | exon | 7739770 | 7739954 | 7739770 | ID=MS.gene24788.t1.exon2;Parent=MS.gene24788.t1 |
| chr8.2 | CDS | 7739770 | 7739954 | 7739770 | ID=cds.MS.gene24788.t1;Parent=MS.gene24788.t1 |
| chr8.2 | exon | 7740067 | 7740127 | 7740067 | ID=MS.gene24788.t1.exon3;Parent=MS.gene24788.t1 |
| chr8.2 | CDS | 7740067 | 7740127 | 7740067 | ID=cds.MS.gene24788.t1;Parent=MS.gene24788.t1 |
| chr8.2 | exon | 7740682 | 7740730 | 7740682 | ID=MS.gene24788.t1.exon4;Parent=MS.gene24788.t1 |
| chr8.2 | CDS | 7740682 | 7740730 | 7740682 | ID=cds.MS.gene24788.t1;Parent=MS.gene24788.t1 |
| Gene Sequence |
| Protein sequence |
