AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene051944

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051944.t1 MTR_2g099990 96.162 469 12 4 20 483 1 468 0.0 929
MS.gene051944.t1 MTR_2g099990 96.000 400 11 3 88 483 1 399 0.0 798
MS.gene051944.t1 MTR_8g017210 58.634 527 131 19 1 465 1 502 4.61e-176 504
MS.gene051944.t1 MTR_1g016010 48.687 495 183 15 12 480 3 452 9.79e-132 389
MS.gene051944.t1 MTR_1g094115 88.415 164 19 0 61 224 44 207 3.82e-110 337
MS.gene051944.t1 MTR_4g059870 42.589 479 191 15 61 475 35 493 9.88e-109 332
MS.gene051944.t1 MTR_1g112270 88.125 160 19 0 67 226 47 206 1.99e-107 331
MS.gene051944.t1 MTR_1g112270 84.337 166 26 0 61 226 52 217 4.73e-104 322
MS.gene051944.t1 MTR_2g093960 81.928 166 29 1 63 227 31 196 5.48e-102 311
MS.gene051944.t1 MTR_2g090745 87.582 153 19 0 74 226 17 169 6.06e-101 309
MS.gene051944.t1 MTR_1g094115 78.049 164 19 1 61 224 44 190 4.97e-93 292
MS.gene051944.t1 MTR_2g017720 60.606 165 51 2 33 188 273 432 1.40e-59 202
MS.gene051944.t1 MTR_3g102000 70.707 99 22 2 91 185 36 131 2.56e-41 147
MS.gene051944.t1 MTR_5g030560 76.000 50 12 0 69 118 11 60 1.35e-21 89.0
MS.gene051944.t1 MTR_1g093095 35.172 145 79 6 85 216 107 249 2.30e-18 85.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051944.t1 AT1G55110 50.105 477 168 15 16 470 17 445 7.15e-131 387
MS.gene051944.t1 AT1G55110 50.105 477 168 15 16 470 17 445 7.15e-131 387
MS.gene051944.t1 AT1G55110 50.105 477 168 15 16 470 17 445 7.15e-131 387
MS.gene051944.t1 AT3G13810 76.233 223 40 3 17 229 17 236 1.32e-117 355
MS.gene051944.t1 AT3G13810 37.356 174 85 8 315 482 358 513 3.74e-18 87.4
MS.gene051944.t1 AT3G13810 76.233 223 40 3 17 229 17 236 1.32e-117 355
MS.gene051944.t1 AT3G13810 37.356 174 85 8 315 482 358 513 3.74e-18 87.4
MS.gene051944.t1 AT3G13810 76.233 223 40 3 17 229 12 231 2.05e-117 354
MS.gene051944.t1 AT3G13810 37.356 174 85 8 315 482 353 508 3.39e-18 87.8
MS.gene051944.t1 AT3G13810 76.364 220 39 3 20 229 1 217 6.55e-116 350
MS.gene051944.t1 AT3G13810 37.356 174 85 8 315 482 339 494 2.65e-18 87.8
MS.gene051944.t1 AT5G66730 73.077 208 39 1 19 226 4 194 3.46e-112 341
MS.gene051944.t1 AT3G50700 78.610 187 30 1 63 249 33 209 4.20e-112 339
MS.gene051944.t1 AT1G03840 88.415 164 19 0 63 226 40 203 7.25e-112 340
MS.gene051944.t1 AT5G44160 54.167 336 95 8 52 334 20 349 1.42e-109 333
MS.gene051944.t1 AT4G02670 71.429 224 55 4 29 243 19 242 1.08e-108 328
MS.gene051944.t1 AT4G02670 71.429 224 55 4 29 243 17 240 1.29e-108 328
MS.gene051944.t1 AT1G14580 67.757 214 64 1 13 226 7 215 2.53e-108 330
MS.gene051944.t1 AT1G14580 67.757 214 64 1 13 226 7 215 2.53e-108 330
MS.gene051944.t1 AT1G14580 67.757 214 64 1 13 226 21 229 4.74e-108 329
MS.gene051944.t1 AT2G02080 83.030 165 28 0 62 226 52 216 4.86e-108 330
MS.gene051944.t1 AT2G02080 83.030 165 28 0 62 226 52 216 4.86e-108 330
MS.gene051944.t1 AT2G02080 83.030 165 28 0 62 226 52 216 4.86e-108 330
MS.gene051944.t1 AT2G02080 83.030 165 28 0 62 226 52 216 4.86e-108 330
MS.gene051944.t1 AT1G03840 87.195 164 19 1 63 226 40 201 5.49e-108 330
MS.gene051944.t1 AT3G45260 82.486 177 28 2 63 236 38 214 1.79e-107 327
MS.gene051944.t1 AT3G45260 82.486 177 28 2 63 236 38 214 1.79e-107 327
MS.gene051944.t1 AT5G60470 76.316 190 42 3 50 237 31 219 4.00e-106 324
MS.gene051944.t1 AT3G13810 69.869 229 50 3 17 229 12 237 4.91e-106 325
MS.gene051944.t1 AT3G13810 37.356 174 85 8 315 482 359 514 4.49e-18 87.4
MS.gene051944.t1 AT5G60470 76.316 190 42 3 50 237 27 215 6.15e-106 323
MS.gene051944.t1 AT2G02070 80.682 176 33 1 64 239 52 226 5.15e-105 325
MS.gene051944.t1 AT2G02070 80.682 176 33 1 64 239 52 226 5.15e-105 325
MS.gene051944.t1 AT3G13810 69.912 226 49 3 20 229 1 223 2.22e-104 321
MS.gene051944.t1 AT3G13810 37.356 174 85 8 315 482 345 500 4.17e-18 87.4
MS.gene051944.t1 AT5G03150 84.242 165 25 1 63 226 52 216 2.70e-103 318
MS.gene051944.t1 AT5G44160 54.045 309 88 7 74 334 8 310 7.33e-101 309
MS.gene051944.t1 AT5G60470 79.394 165 32 2 75 237 46 210 8.18e-97 300
MS.gene051944.t1 AT2G02080 83.453 139 23 0 88 226 1 139 3.75e-91 285
MS.gene051944.t1 AT2G02080 83.453 139 23 0 88 226 1 139 3.75e-91 285
MS.gene051944.t1 AT1G68130 64.103 156 54 2 64 217 41 196 1.52e-72 236
MS.gene051944.t1 AT2G01940 60.345 174 61 3 64 235 44 211 3.42e-72 236
MS.gene051944.t1 AT1G25250 58.011 181 62 4 64 241 33 202 4.78e-72 233
MS.gene051944.t1 AT1G25250 59.064 171 56 4 74 241 20 179 1.07e-68 224
MS.gene051944.t1 AT2G01940 59.429 175 62 4 64 235 44 212 1.26e-68 226
MS.gene051944.t1 AT1G68130 64.545 110 37 2 110 217 1 110 7.76e-46 163
MS.gene051944.t1 AT2G01940 58.594 128 45 3 110 235 1 122 9.80e-46 163
MS.gene051944.t1 AT1G25250 55.474 137 47 4 110 243 1 126 1.56e-45 161
MS.gene051944.t1 AT1G34370 31.308 214 124 6 59 254 3 211 5.93e-24 102
MS.gene051944.t1 AT1G34370 30.876 217 127 6 56 254 210 421 9.52e-24 104
MS.gene051944.t1 AT1G34370 30.876 217 127 6 56 254 210 421 9.52e-24 104
MS.gene051944.t1 AT5G22890 32.026 153 90 3 78 216 202 354 1.77e-21 96.3
MS.gene051944.t1 AT1G13290 34.161 161 91 7 76 224 85 242 4.52e-19 88.2
MS.gene051944.t1 AT3G20880 33.562 146 87 4 80 216 243 387 2.49e-18 87.4
MS.gene051944.t1 AT1G51220 32.192 146 89 4 80 216 165 309 6.85e-18 85.1
MS.gene051944.t1 AT3G57670 32.653 147 87 5 80 216 204 348 9.83e-18 85.5
MS.gene051944.t1 AT1G08290 36.765 136 73 6 92 216 179 312 6.93e-17 82.4
MS.gene051944.t1 AT1G34790 32.450 151 90 5 76 216 128 276 4.23e-16 79.3

Find 97 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCTTCTTCTGGATTTCA+TGG 0.185525 2.2:-3085308 MS.gene051944:CDS
TTCTCTTGTGCCACAGGTTT+TGG 0.189840 2.2:+3086401 None:intergenic
AACAACTCTTTCTCTTCTTC+TGG 0.209031 2.2:-3085317 MS.gene051944:CDS
TTCATGTTGTGATGAGAATT+TGG 0.245330 2.2:+3085758 None:intergenic
GGTATTTGTACTTCTTGTTC+TGG 0.297642 2.2:+3085665 None:intergenic
GAAGCTAGTGCTAATTCTTC+TGG 0.298581 2.2:-3087323 MS.gene051944:CDS
GGAAGTGTAGGACCATTTCT+AGG 0.313383 2.2:+3085557 None:intergenic
ATCTCTCTTCAAACTATTCA+AGG 0.324026 2.2:-3085616 MS.gene051944:CDS
TTTGTTGCCATCAGTGTCTT+TGG 0.324056 2.2:+3086714 None:intergenic
AGCCTTGGATATCTTGAGTT+TGG 0.330060 2.2:+3085729 None:intergenic
GCACTTACAAGAGATTTCTT+AGG 0.351186 2.2:-3085149 MS.gene051944:CDS
AGAAAGAGTTGTTTATCATT+TGG 0.355557 2.2:+3085327 None:intergenic
GTAGGACCATTTCTAGGGTT+AGG 0.377918 2.2:+3085563 None:intergenic
ATCAAAGTTTCAAAATGTTA+AGG 0.407806 2.2:-3085688 MS.gene051944:CDS
AGTGATATCCTCACCATTGC+TGG 0.409995 2.2:-3085107 MS.gene051944:CDS
TCTAATTTGACTTCTGCATC+TGG 0.412009 2.2:-3087347 MS.gene051944:CDS
TAGGACCATTTCTAGGGTTA+GGG 0.414611 2.2:+3085564 None:intergenic
TGGTGCACACAAGTTGGTTC+TGG 0.417382 2.2:+3086555 None:intergenic
TATCCTCACCATTGCTGGTA+TGG 0.421802 2.2:-3085102 MS.gene051944:CDS
CATGACCCATCAAGAGCCTT+GGG 0.426349 2.2:-3086534 MS.gene051944:CDS
GTTGAGTGGGACCCACCAAC+AGG 0.427753 2.2:-3085435 MS.gene051944:CDS
CTTTCTTCAATGTGAAGCCT+TGG 0.431323 2.2:+3085714 None:intergenic
GTTCTTTGCTTCAACTTCCA+TGG 0.431659 2.2:+3086612 None:intergenic
TCTGTATTCTCTTGTGCCAC+AGG 0.435175 2.2:+3086395 None:intergenic
AAGAAAAGAAGAAACCTTCC+TGG 0.442018 2.2:-3087218 MS.gene051944:CDS
ATTTGAGTTTATCATGTTGT+TGG 0.445102 2.2:+3085385 None:intergenic
AAGCTATAACTTCAGCTTGT+GGG 0.447498 2.2:+3086742 None:intergenic
GAAAACCCTAACCCTAGAAA+TGG 0.454115 2.2:-3085569 MS.gene051944:CDS
AGTGCTTCTTGATACCAGTT+AGG 0.454887 2.2:+3086508 None:intergenic
TGATGAGAATTTGGCTGTGT+AGG 0.455107 2.2:+3085767 None:intergenic
GAAAATGAACCTGGATTGCC+AGG 0.461039 2.2:+3087200 None:intergenic
CTCTTCAAACTATTCAAGGT+TGG 0.466017 2.2:-3085612 MS.gene051944:CDS
TGCATTGTTTCCTCCGGCAC+CGG 0.471541 2.2:+3085169 None:intergenic
AGAAACCTTCCTGGCAATCC+AGG 0.472577 2.2:-3087209 MS.gene051944:intron
CCAAGGCTCTTGATGGGTCA+TGG 0.475409 2.2:+3086535 None:intergenic
TGTAAGTGCATTGTTTCCTC+CGG 0.475628 2.2:+3085163 None:intergenic
ATAGTTTGAAGAGAGATCAA+TGG 0.482584 2.2:+3085622 None:intergenic
CCATGACCCATCAAGAGCCT+TGG 0.490603 2.2:-3086535 MS.gene051944:CDS
ACCGGCACCGTCATCATCAT+TGG 0.490777 2.2:+3085187 None:intergenic
AACAACATGATAAACTCAAA+TGG 0.493838 2.2:-3085383 MS.gene051944:CDS
AATCTTCAACTTCATAAAAG+AGG 0.500458 2.2:-3086645 MS.gene051944:CDS
ATCCTCACCATTGCTGGTAT+GGG 0.502220 2.2:-3085101 MS.gene051944:CDS
AGTTGATGCAAAATTCTCTC+AGG 0.503692 2.2:-3087288 MS.gene051944:CDS
ATAGTTATACCTTGAGAAGA+GGG 0.512121 2.2:+3086359 None:intergenic
GATGATGACGGTGCCGGTGC+CGG 0.516820 2.2:-3085182 MS.gene051944:CDS
GTTACAGAAAGCAGCTCAGA+TGG 0.517581 2.2:-3085483 MS.gene051944:CDS
ATGAACCTGGATTGCCAGGA+AGG 0.520133 2.2:+3087204 None:intergenic
ATATGTGAGATCTGTAACAA+AGG 0.526520 2.2:-3086684 MS.gene051944:CDS
AAGAAGTACAAATACCCTCT+TGG 0.528888 2.2:-3085658 MS.gene051944:CDS
ACAACAATCATTAGTTGTTG+AGG 0.530940 2.2:-3087378 MS.gene051944:CDS
AGTATGCAGTTCAATCAGAC+TGG 0.532991 2.2:-3086434 MS.gene051944:CDS
AAAGAACAAGCAATGAGATA+AGG 0.533182 2.2:-3086596 MS.gene051944:CDS
TGAGTTTATCATGTTGTTGG+TGG 0.541113 2.2:+3085388 None:intergenic
GCAGATGATGGTTGGTAGGA+TGG 0.545269 2.2:+3085536 None:intergenic
TTGGTGGGTCCCACTCAACA+TGG 0.546559 2.2:+3085439 None:intergenic
CCAAACTAATTACCTTCCCA+TGG 0.548476 2.2:+3085026 None:intergenic
CAACATGGACTGAGATGAGC+TGG 0.548999 2.2:+3085454 None:intergenic
TATCATTTGGTCTTCACATG+AGG 0.559455 2.2:+3085340 None:intergenic
TATAGTTATACCTTGAGAAG+AGG 0.562344 2.2:+3086358 None:intergenic
GAACATAATATCAATCATGA+TGG 0.565329 2.2:-3085236 MS.gene051944:CDS
GAAAGCACGGTGAGAAGAAG+TGG 0.566601 2.2:-3086479 MS.gene051944:CDS
GAAGTGTAGGACCATTTCTA+GGG 0.573649 2.2:+3085558 None:intergenic
TCATCTCAGTCCATGTTGAG+TGG 0.578988 2.2:-3085449 MS.gene051944:CDS
TGAGGTGAAGCTGCAGATGA+TGG 0.582967 2.2:+3085524 None:intergenic
TAGACACGTGACCCTGTTGG+TGG 0.583962 2.2:+3085423 None:intergenic
GGCACCGTCATCATCATTGG+TGG 0.585980 2.2:+3085190 None:intergenic
AAAGAGGACACAATTTGCCA+TGG 0.587343 2.2:-3086629 MS.gene051944:CDS
CATCTCAGTCCATGTTGAGT+GGG 0.587878 2.2:-3085448 MS.gene051944:CDS
CTCAGCTAATGCATCACAGA+AGG 0.590439 2.2:+3085817 None:intergenic
CTCCAAACTCAAGATATCCA+AGG 0.596098 2.2:-3085731 MS.gene051944:CDS
GCAACTTCCCATACCAGCAA+TGG 0.602937 2.2:+3085094 None:intergenic
AAAGCTATAACTTCAGCTTG+TGG 0.603171 2.2:+3086741 None:intergenic
AAGCACTTCTGCAGAAAGCA+CGG 0.603251 2.2:-3086492 MS.gene051944:CDS
GATGATTGACAAAGCCAAGA+GGG 0.607399 2.2:+3085644 None:intergenic
GAATTTGGCTGTGTAGGTTG+AGG 0.609498 2.2:+3085773 None:intergenic
GATGACGGTGCCGGTGCCGG+AGG 0.615538 2.2:-3085179 MS.gene051944:CDS
TTCCCATACCAGCAATGGTG+AGG 0.621943 2.2:+3085099 None:intergenic
CAATAGACACGTGACCCTGT+TGG 0.624582 2.2:+3085420 None:intergenic
ACCAATGATGATGACGGTGC+CGG 0.625084 2.2:-3085188 MS.gene051944:CDS
ACTGTGGAACCCTCTTCTCA+AGG 0.626939 2.2:-3086368 MS.gene051944:intron
TCAACCACCAATGATGATGA+CGG 0.633146 2.2:-3085194 MS.gene051944:CDS
TTACAGAAAGCAGCTCAGAT+GGG 0.645481 2.2:-3085482 MS.gene051944:CDS
GGTTGGTAGGATGGAAGTGT+AGG 0.650505 2.2:+3085545 None:intergenic
AGACACGTGACCCTGTTGGT+GGG 0.651658 2.2:+3085424 None:intergenic
TGACCCATCAAGAGCCTTGG+GGG 0.652313 2.2:-3086532 MS.gene051944:CDS
GTGAAGCTGCAGATGATGGT+TGG 0.652944 2.2:+3085528 None:intergenic
TTTGTCACCAAAGACACTGA+TGG 0.656547 2.2:-3086721 MS.gene051944:CDS
AGGATATCACTATCAGAGAG+AGG 0.661126 2.2:+3085119 None:intergenic
AGCTGCAGATGATGGTTGGT+AGG 0.662572 2.2:+3085532 None:intergenic
CTTCTGTGATGCATTAGCTG+AGG 0.666815 2.2:-3085816 MS.gene051944:CDS
GGGTCATGGTGCACACAAGT+TGG 0.674016 2.2:+3086549 None:intergenic
TTGAGTGGGACCCACCAACA+GGG 0.675919 2.2:-3085434 MS.gene051944:CDS
AAAGCTCACTCCAAAACCTG+TGG 0.698299 2.2:-3086411 MS.gene051944:CDS
AGAGAATACAGATGTGACTG+TGG 0.700052 2.2:-3086384 MS.gene051944:CDS
GGATGATTGACAAAGCCAAG+AGG 0.702136 2.2:+3085643 None:intergenic
ATGACCCATCAAGAGCCTTG+GGG 0.705499 2.2:-3086533 MS.gene051944:CDS
AATGCTGTAGCTGACATGTG+AGG 0.760209 2.2:+3085506 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATATATTATATGATTTTC+AGG - chr2.2:3086573-3086592 MS.gene051944:CDS 10.0%
!!! ATTATTTTATTAAACTGTTT+AGG + chr2.2:3086120-3086139 None:intergenic 10.0%
!! ATCTTAATTGTTATAAATTG+TGG - chr2.2:3085286-3085305 MS.gene051944:CDS 15.0%
!!! CATATTTTGTTTATCTAAAT+TGG - chr2.2:3085625-3085644 MS.gene051944:CDS 15.0%
!! ATCAAAGTTTCAAAATGTTA+AGG - chr2.2:3086750-3086769 MS.gene051944:intron 20.0%
!! ATTAAGATGAAAAGAAAAGA+TGG + chr2.2:3085274-3085293 None:intergenic 20.0%
!! GAAAAATATGAAGAAAAACA+TGG - chr2.2:3085352-3085371 MS.gene051944:CDS 20.0%
!! TATACTATTATCAACTTGAA+GGG + chr2.2:3086158-3086177 None:intergenic 20.0%
!!! ATATTATATGATTTTCAGGA+GGG - chr2.2:3086577-3086596 MS.gene051944:CDS 20.0%
!!! CAAACATTTTTTAAAGAAAG+TGG - chr2.2:3086209-3086228 MS.gene051944:intron 20.0%
!!! GAAAGTGGAAATTTTTAAAA+TGG - chr2.2:3086224-3086243 MS.gene051944:intron 20.0%
!!! TATATTATATGATTTTCAGG+AGG - chr2.2:3086576-3086595 MS.gene051944:CDS 20.0%
! AACAACATGATAAACTCAAA+TGG - chr2.2:3087055-3087074 MS.gene051944:intron 25.0%
! AATCTTCAACTTCATAAAAG+AGG - chr2.2:3085793-3085812 MS.gene051944:CDS 25.0%
! ACTTAGTAATCTCATGTATA+AGG - chr2.2:3086432-3086451 MS.gene051944:CDS 25.0%
! AGAAAGAGTTGTTTATCATT+TGG + chr2.2:3087114-3087133 None:intergenic 25.0%
! GAACATAATATCAATCATGA+TGG - chr2.2:3087202-3087221 MS.gene051944:intron 25.0%
! GTATACTATTATCAACTTGA+AGG + chr2.2:3086159-3086178 None:intergenic 25.0%
! TTTAACATGTTATGCTTGTT+TGG - chr2.2:3085473-3085492 MS.gene051944:CDS 25.0%
!! ATTTGAGTTTATCATGTTGT+TGG + chr2.2:3087056-3087075 None:intergenic 25.0%
!! CATATTTTTCCACAATATCA+TGG + chr2.2:3085342-3085361 None:intergenic 25.0%
!! TTGGATAAATGTGAATTTTC+CGG + chr2.2:3086407-3086426 None:intergenic 25.0%
AAAGAACAAGCAATGAGATA+AGG - chr2.2:3085842-3085861 MS.gene051944:CDS 30.0%
ACAACAATCATTAGTTGTTG+AGG - chr2.2:3085060-3085079 MS.gene051944:CDS 30.0%
ACTTGAAGGGATAAAAAATG+TGG + chr2.2:3086145-3086164 None:intergenic 30.0%
AGAACTTAAGAAAATGAACC+TGG + chr2.2:3085250-3085269 None:intergenic 30.0%
ATAAATTGTGGTTATAGTCG+CGG - chr2.2:3085298-3085317 MS.gene051944:CDS 30.0%
ATAGTTATACCTTGAGAAGA+GGG + chr2.2:3086082-3086101 None:intergenic 30.0%
ATAGTTTGAAGAGAGATCAA+TGG + chr2.2:3086819-3086838 None:intergenic 30.0%
ATATGTGAGATCTGTAACAA+AGG - chr2.2:3085754-3085773 MS.gene051944:CDS 30.0%
ATCTCTCTTCAAACTATTCA+AGG - chr2.2:3086822-3086841 MS.gene051944:intron 30.0%
ATTGTGAATCAGAAAATGAG+AGG - chr2.2:3086475-3086494 MS.gene051944:CDS 30.0%
CAACAAAATCATACTAACAC+CGG - chr2.2:3086385-3086404 MS.gene051944:CDS 30.0%
GCAATGACATCTAATTAAAC+CGG - chr2.2:3085409-3085428 MS.gene051944:CDS 30.0%
TATAGTTATACCTTGAGAAG+AGG + chr2.2:3086083-3086102 None:intergenic 30.0%
TTAATTAGATGTCATTGCAG+TGG + chr2.2:3085407-3085426 None:intergenic 30.0%
TTCATGTTGTGATGAGAATT+TGG + chr2.2:3086683-3086702 None:intergenic 30.0%
TTGTGAATCAGAAAATGAGA+GGG - chr2.2:3086476-3086495 MS.gene051944:CDS 30.0%
! ATTTCATGGAACTTCTTTTG+AGG - chr2.2:3087144-3087163 MS.gene051944:intron 30.0%
! TTTTTAATGTACGATCTCTG+TGG - chr2.2:3086258-3086277 MS.gene051944:intron 30.0%
AAAACCATTCCCAAAAACCA+TGG - chr2.2:3087395-3087414 MS.gene051944:CDS 35.0%
AAACCATTCCCAAAAACCAT+GGG - chr2.2:3087396-3087415 MS.gene051944:CDS 35.0%
AAAGCTATAACTTCAGCTTG+TGG + chr2.2:3085700-3085719 None:intergenic 35.0%
AACAACTCTTTCTCTTCTTC+TGG - chr2.2:3087121-3087140 MS.gene051944:intron 35.0%
AAGAAAAGAAGAAACCTTCC+TGG - chr2.2:3085220-3085239 MS.gene051944:CDS 35.0%
AAGCTATAACTTCAGCTTGT+GGG + chr2.2:3085699-3085718 None:intergenic 35.0%
ATAGTGCATGCTTAGATTGA+TGG - chr2.2:3086291-3086310 MS.gene051944:intron 35.0%
CTCAAGCTTCAACAAAATCA+CGG + chr2.2:3085529-3085548 None:intergenic 35.0%
CTCTTCAAACTATTCAAGGT+TGG - chr2.2:3086826-3086845 MS.gene051944:intron 35.0%
GCACTTACAAGAGATTTCTT+AGG - chr2.2:3087289-3087308 MS.gene051944:CDS 35.0%
GGTATTTGTACTTCTTGTTC+TGG + chr2.2:3086776-3086795 None:intergenic 35.0%
TGAGATTACTAAGTGCATGT+TGG + chr2.2:3086426-3086445 None:intergenic 35.0%
TGAGTTTATCATGTTGTTGG+TGG + chr2.2:3087053-3087072 None:intergenic 35.0%
TTCTCTTCTTCTGGATTTCA+TGG - chr2.2:3087130-3087149 MS.gene051944:intron 35.0%
! AAGAAGTACAAATACCCTCT+TGG - chr2.2:3086780-3086799 MS.gene051944:intron 35.0%
! AATTACCTTCCCATGGTTTT+TGG + chr2.2:3087408-3087427 None:intergenic 35.0%
! AGGTTTCTTCTTTTCTTAGC+TGG + chr2.2:3085217-3085236 None:intergenic 35.0%
! AGTTGATGCAAAATTCTCTC+AGG - chr2.2:3085150-3085169 MS.gene051944:CDS 35.0%
! ATTACCTTCCCATGGTTTTT+GGG + chr2.2:3087407-3087426 None:intergenic 35.0%
! GAAGAAAAACATGGTTGATG+CGG - chr2.2:3085361-3085380 MS.gene051944:CDS 35.0%
! GTTATGCTTGTTTGGAATCA+TGG - chr2.2:3085481-3085500 MS.gene051944:CDS 35.0%
! TAGGGTTAGGGTTTTCATAA+AGG + chr2.2:3086865-3086884 None:intergenic 35.0%
! TATCATTTGGTCTTCACATG+AGG + chr2.2:3087101-3087120 None:intergenic 35.0%
! TCTAATTTGACTTCTGCATC+TGG - chr2.2:3085091-3085110 MS.gene051944:CDS 35.0%
!! GGAATGGTTTTCTTGATGAT+CGG + chr2.2:3087386-3087405 None:intergenic 35.0%
!! TCTTTTGAGGATACTTTTGC+TGG - chr2.2:3087157-3087176 MS.gene051944:intron 35.0%
AAAGAGGACACAATTTGCCA+TGG - chr2.2:3085809-3085828 MS.gene051944:CDS 40.0%
AAGCTTCAACAAAATCACGG+TGG + chr2.2:3085526-3085545 None:intergenic 40.0%
AATATGAATGCGCGCATGTT+TGG + chr2.2:3085611-3085630 None:intergenic 40.0%
AGAGAATACAGATGTGACTG+TGG - chr2.2:3086054-3086073 MS.gene051944:intron 40.0%
AGCCTTGGATATCTTGAGTT+TGG + chr2.2:3086712-3086731 None:intergenic 40.0%
AGGATATCACTATCAGAGAG+AGG + chr2.2:3087322-3087341 None:intergenic 40.0%
AGTATGCAGTTCAATCAGAC+TGG - chr2.2:3086004-3086023 MS.gene051944:intron 40.0%
AGTGCTTCTTGATACCAGTT+AGG + chr2.2:3085933-3085952 None:intergenic 40.0%
CGCTATGAACCATGATATTG+TGG - chr2.2:3085330-3085349 MS.gene051944:CDS 40.0%
CTCCAAACTCAAGATATCCA+AGG - chr2.2:3086707-3086726 MS.gene051944:CDS 40.0%
CTTTCTTCAATGTGAAGCCT+TGG + chr2.2:3086727-3086746 None:intergenic 40.0%
GAAAACCCTAACCCTAGAAA+TGG - chr2.2:3086869-3086888 MS.gene051944:intron 40.0%
GAAGCTAGTGCTAATTCTTC+TGG - chr2.2:3085115-3085134 MS.gene051944:CDS 40.0%
GAAGTGTAGGACCATTTCTA+GGG + chr2.2:3086883-3086902 None:intergenic 40.0%
GATGATTGACAAAGCCAAGA+GGG + chr2.2:3086797-3086816 None:intergenic 40.0%
GTTCTTTGCTTCAACTTCCA+TGG + chr2.2:3085829-3085848 None:intergenic 40.0%
TGATGAGAATTTGGCTGTGT+AGG + chr2.2:3086674-3086693 None:intergenic 40.0%
TGGCATCACAACTACAATTG+CGG + chr2.2:3085387-3085406 None:intergenic 40.0%
TGTAAGTGCATTGTTTCCTC+CGG + chr2.2:3087278-3087297 None:intergenic 40.0%
TGTACGATCTCTGTGGTTAT+CGG - chr2.2:3086265-3086284 MS.gene051944:intron 40.0%
TTACAGAAAGCAGCTCAGAT+GGG - chr2.2:3086956-3086975 MS.gene051944:intron 40.0%
TTTGTCACCAAAGACACTGA+TGG - chr2.2:3085717-3085736 MS.gene051944:CDS 40.0%
TTTGTTGCCATCAGTGTCTT+TGG + chr2.2:3085727-3085746 None:intergenic 40.0%
! TCAACCACCAATGATGATGA+CGG - chr2.2:3087244-3087263 MS.gene051944:CDS 40.0%
!! GTTTTCTTGATGATCGGAGT+TGG + chr2.2:3087380-3087399 None:intergenic 40.0%
!! TAGGACCATTTCTAGGGTTA+GGG + chr2.2:3086877-3086896 None:intergenic 40.0%
AAAGCTCACTCCAAAACCTG+TGG - chr2.2:3086027-3086046 MS.gene051944:intron 45.0%
AAGCACTTCTGCAGAAAGCA+CGG - chr2.2:3085946-3085965 MS.gene051944:intron 45.0%
AATGCTGTAGCTGACATGTG+AGG + chr2.2:3086935-3086954 None:intergenic 45.0%
AGTGATATCCTCACCATTGC+TGG - chr2.2:3087331-3087350 MS.gene051944:CDS 45.0%
ATCCTCACCATTGCTGGTAT+GGG - chr2.2:3087337-3087356 MS.gene051944:CDS 45.0%
CATCTCAGTCCATGTTGAGT+GGG - chr2.2:3086990-3087009 MS.gene051944:intron 45.0%
CATTCCCAAAAACCATGGGA+AGG - chr2.2:3087400-3087419 MS.gene051944:CDS 45.0%
CGTTTGCAGCAGCAATATAC+CGG + chr2.2:3085431-3085450 None:intergenic 45.0%
CTCAGCTAATGCATCACAGA+AGG + chr2.2:3086624-3086643 None:intergenic 45.0%
CTTCTGTGATGCATTAGCTG+AGG - chr2.2:3086622-3086641 MS.gene051944:CDS 45.0%
GAAAATGAACCTGGATTGCC+AGG + chr2.2:3085241-3085260 None:intergenic 45.0%
GAATTTGGCTGTGTAGGTTG+AGG + chr2.2:3086668-3086687 None:intergenic 45.0%
GGATGATTGACAAAGCCAAG+AGG + chr2.2:3086798-3086817 None:intergenic 45.0%
GTTACAGAAAGCAGCTCAGA+TGG - chr2.2:3086955-3086974 MS.gene051944:intron 45.0%
TATCCTCACCATTGCTGGTA+TGG - chr2.2:3087336-3087355 MS.gene051944:CDS 45.0%
TCATCTCAGTCCATGTTGAG+TGG - chr2.2:3086989-3087008 MS.gene051944:intron 45.0%
TCTGTATTCTCTTGTGCCAC+AGG + chr2.2:3086046-3086065 None:intergenic 45.0%
! GGAAGTGTAGGACCATTTCT+AGG + chr2.2:3086884-3086903 None:intergenic 45.0%
! TTCTCTTGTGCCACAGGTTT+TGG + chr2.2:3086040-3086059 None:intergenic 45.0%
!! CTTCCCATGGTTTTTGGGAA+TGG + chr2.2:3087402-3087421 None:intergenic 45.0%
!! GTAGGACCATTTCTAGGGTT+AGG + chr2.2:3086878-3086897 None:intergenic 45.0%
ACTGTGGAACCCTCTTCTCA+AGG - chr2.2:3086070-3086089 MS.gene051944:intron 50.0%
AGAAACCTTCCTGGCAATCC+AGG - chr2.2:3085229-3085248 MS.gene051944:CDS 50.0%
ATGAACCTGGATTGCCAGGA+AGG + chr2.2:3085237-3085256 None:intergenic 50.0%
ATGACCCATCAAGAGCCTTG+GGG - chr2.2:3085905-3085924 MS.gene051944:intron 50.0%
CAATAGACACGTGACCCTGT+TGG + chr2.2:3087021-3087040 None:intergenic 50.0%
CATGACCCATCAAGAGCCTT+GGG - chr2.2:3085904-3085923 MS.gene051944:intron 50.0%
GCAACTTCCCATACCAGCAA+TGG + chr2.2:3087347-3087366 None:intergenic 50.0%
GCGTAGCTTCTGCAAACTCA+CGG - chr2.2:3085550-3085569 MS.gene051944:CDS 50.0%
TGAGGTGAAGCTGCAGATGA+TGG + chr2.2:3086917-3086936 None:intergenic 50.0%
TTCCCATACCAGCAATGGTG+AGG + chr2.2:3087342-3087361 None:intergenic 50.0%
! AGCTGCAGATGATGGTTGGT+AGG + chr2.2:3086909-3086928 None:intergenic 50.0%
! CAACATGGACTGAGATGAGC+TGG + chr2.2:3086987-3087006 None:intergenic 50.0%
! GAAAGCACGGTGAGAAGAAG+TGG - chr2.2:3085959-3085978 MS.gene051944:intron 50.0%
! GTGAAGCTGCAGATGATGGT+TGG + chr2.2:3086913-3086932 None:intergenic 50.0%
!! ACCAATGATGATGACGGTGC+CGG - chr2.2:3087250-3087269 MS.gene051944:CDS 50.0%
!! GCAGATGATGGTTGGTAGGA+TGG + chr2.2:3086905-3086924 None:intergenic 50.0%
!! GGTTGGTAGGATGGAAGTGT+AGG + chr2.2:3086896-3086915 None:intergenic 50.0%
!! TGGTGCACACAAGTTGGTTC+TGG + chr2.2:3085886-3085905 None:intergenic 50.0%
ACCGGCACCGTCATCATCAT+TGG + chr2.2:3087254-3087273 None:intergenic 55.0%
AGACACGTGACCCTGTTGGT+GGG + chr2.2:3087017-3087036 None:intergenic 55.0%
CCATGACCCATCAAGAGCCT+TGG - chr2.2:3085903-3085922 MS.gene051944:intron 55.0%
GATACCAGTTAGGTCCCCCA+AGG + chr2.2:3085923-3085942 None:intergenic 55.0%
GGCACCGTCATCATCATTGG+TGG + chr2.2:3087251-3087270 None:intergenic 55.0%
GGGTCATGGTGCACACAAGT+TGG + chr2.2:3085892-3085911 None:intergenic 55.0%
TAGACACGTGACCCTGTTGG+TGG + chr2.2:3087018-3087037 None:intergenic 55.0%
TGACCCATCAAGAGCCTTGG+GGG - chr2.2:3085906-3085925 MS.gene051944:intron 55.0%
TGCATTGTTTCCTCCGGCAC+CGG + chr2.2:3087272-3087291 None:intergenic 55.0%
TTGAGTGGGACCCACCAACA+GGG - chr2.2:3087004-3087023 MS.gene051944:intron 55.0%
! CCAAGGCTCTTGATGGGTCA+TGG + chr2.2:3085906-3085925 None:intergenic 55.0%
! TTGGTGGGTCCCACTCAACA+TGG + chr2.2:3087002-3087021 None:intergenic 55.0%
AGAGCCTTGGGGGACCTAAC+TGG - chr2.2:3085916-3085935 MS.gene051944:intron 60.0%
AGGTCCCCCAAGGCTCTTGA+TGG + chr2.2:3085913-3085932 None:intergenic 60.0%
GGTCCCCCAAGGCTCTTGAT+GGG + chr2.2:3085912-3085931 None:intergenic 60.0%
GTTGAGTGGGACCCACCAAC+AGG - chr2.2:3087003-3087022 MS.gene051944:intron 60.0%
!! GATGATGACGGTGCCGGTGC+CGG - chr2.2:3087256-3087275 MS.gene051944:CDS 65.0%
!! GATGACGGTGCCGGTGCCGG+AGG - chr2.2:3087259-3087278 MS.gene051944:CDS 75.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 3085031 3087429 3085031 ID=MS.gene051944
chr2.2 mRNA 3085031 3087429 3085031 ID=MS.gene051944.t1;Parent=MS.gene051944
chr2.2 exon 3087210 3087429 3087210 ID=MS.gene051944.t1.exon1;Parent=MS.gene051944.t1
chr2.2 CDS 3087210 3087429 3087210 ID=cds.MS.gene051944.t1;Parent=MS.gene051944.t1
chr2.2 exon 3086369 3086765 3086369 ID=MS.gene051944.t1.exon2;Parent=MS.gene051944.t1
chr2.2 CDS 3086369 3086765 3086369 ID=cds.MS.gene051944.t1;Parent=MS.gene051944.t1
chr2.2 exon 3085031 3085865 3085031 ID=MS.gene051944.t1.exon3;Parent=MS.gene051944.t1
chr2.2 CDS 3085031 3085865 3085031 ID=cds.MS.gene051944.t1;Parent=MS.gene051944.t1
Gene Sequence

>MS.gene051944

ATGATCAAAGGTTTTATGTCTCAACAAGAACAACAATCATTAGTTGTTGAGGAAAACATGTCTAATTTGACTTCTGCATCTGGTGAAGCTAGTGCTAATTCTTCTGGAAACAGAACTGAAGTTGATGCAAAATTCTCTCAGGAATACTTTGCTTCATCACAAACTCAAACTCATGATGAGACTCCAGCTAAGAAAAGAAGAAACCTTCCTGGCAATCCAGGTTCATTTTCTTAAGTTCTACCATCTTTTCTTTTCATCTTAATTGTTATAAATTGTGGTTATAGTCGCGGTTATGATTACGCTATGAACCATGATATTGTGGAAAAATATGAAGAAAAACATGGTTGATGCGGCCGCAATTGTAGTTGTGATGCCACTGCAATGACATCTAATTAAACCGGTATATTGCTGCTGCAAACGACAATTTAGAACTATATGTGTATTTAACATGTTATGCTTGTTTGGAATCATGGTATAGATTTGACAAAGTTACCACCGTGATTTTGTTGAAGCTTGAGAGCGTAGCTTCTGCAAACTCACGGTGACTGACAGTAATTTTATCAAACTCATCGTGAATCCAAACATGCGCGCATTCATATTTTGTTTATCTAAATTGGATTTCTTTTGTTTTGTGTTTGTTTTTTTATTTGATTATTCATTTTAGACCCACAAGCTGAAGTTATAGCTTTGTCACCAAAGACACTGATGGCAACAAATAGATTCATATGTGAGATCTGTAACAAAGGATTTCAAAGAGATCAGAATCTTCAACTTCATAAAAGAGGACACAATTTGCCATGGAAGTTGAAGCAAAGAACAAGCAATGAGATAAGGAAGAAAGTGTATGTGTGTCCAGAACCAACTTGTGTGCACCATGACCCATCAAGAGCCTTGGGGGACCTAACTGGTATCAAGAAGCACTTCTGCAGAAAGCACGGTGAGAAGAAGTGGAAATGTGACAAGTGCTCTAAGAAGTATGCAGTTCAATCAGACTGGAAAGCTCACTCCAAAACCTGTGGCACAAGAGAATACAGATGTGACTGTGGAACCCTCTTCTCAAGGTATAACTATAAATATATTAACTAACCTAAACAGTTTAATAAAATAATTACCACATTTTTTATCCCTTCAAGTTGATAATAGTATACTGAACATGTAAAAAGAGTTCAATACATAGTCAAACATTTTTTAAAGAAAGTGGAAATTTTTAAAATGGAAAATTGAATTTTTTTAATGTACGATCTCTGTGGTTATCGGTGCATAGTGCATGCTTAGATTGATGGTGAATTTCTCAGAATCATTTAGAATCACATTGTATCATCTTAATTTTGACAAAAGTTATACTTCAGAGTTTCAACAAAATCATACTAACACCGGAAAATTCACATTTATCCAACATGCACTTAGTAATCTCATGTATAAGGTGAGTGAGACTAAAAAAATGATTGTGAATCAGAAAATGAGAGGGTGAATATTTCTTGACTGTTATAGCATTTTAGTAACATTGTTCACTTTTGTATTTTTGTGTTTGTTTATATATATATATATATTATATGATTTTCAGGAGGGACAGTTTCATAACTCACAGAGCCTTCTGTGATGCATTAGCTGAGGAGAGTTCAAGAACTGTAATTCCTCAACCTACACAGCCAAATTCTCATCACAACATGAACAATCTCCAAACTCAAGATATCCAAGGCTTCACATTGAAGAAAGAGCATCAAAGTTTCAAAATGTTAAGGCCAGAACAAGAAGTACAAATACCCTCTTGGCTTTGTCAATCATCCATTGATCTCTCTTCAAACTATTCAAGGTTGGATCAAGATCTACACCTTTATGAAAACCCTAACCCTAGAAATGGTCCTACACTTCCATCCTACCAACCATCATCTGCAGCTTCACCTCACATGTCAGCTACAGCATTGTTACAGAAAGCAGCTCAGATGGGTGCAACCAGCTCATCTCAGTCCATGTTGAGTGGGACCCACCAACAGGGTCACGTGTCTATTGTTGACTCTGCCACCAACAACATGATAAACTCAAATGGTAATTTTAGTTTGAATTTGTCCTCATGTGAAGACCAAATGATAAACAACTCTTTCTCTTCTTCTGGATTTCATGGAACTTCTTTTGAGGATACTTTTGCTGGAAATATTCTTCATTCAAATCAAGAACATAATATCAATCATGATGGTAATAATGATATTACAAAATCAACCACCAATGATGATGACGGTGCCGGTGCCGGAGGAAACAATGCACTTACAAGAGATTTCTTAGGTCTTAAACCTCTCTCTGATAGTGATATCCTCACCATTGCTGGTATGGGAAGTTGCATGAACTCTTCCAACTCCGATCATCAAGAAAACCATTCCCAAAAACCATGGGAAGGTAATTAG
Protein sequence

>MS.gene051944.t1

MIKGFMSQQEQQSLVVEENMSNLTSASGEASANSSGNRTEVDAKFSQEYFASSQTQTHDETPAKKRRNLPGNPDPQAEVIALSPKTLMATNRFICEICNKGFQRDQNLQLHKRGHNLPWKLKQRTSNEIRKKVYVCPEPTCVHHDPSRALGDLTGIKKHFCRKHGEKKWKCDKCSKKYAVQSDWKAHSKTCGTREYRCDCGTLFSRRDSFITHRAFCDALAEESSRTVIPQPTQPNSHHNMNNLQTQDIQGFTLKKEHQSFKMLRPEQEVQIPSWLCQSSIDLSSNYSRLDQDLHLYENPNPRNGPTLPSYQPSSAASPHMSATALLQKAAQMGATSSSQSMLSGTHQQGHVSIVDSATNNMINSNGNFSLNLSSCEDQMINNSFSSSGFHGTSFEDTFAGNILHSNQEHNINHDGNNDITKSTTNDDDGAGAGGNNALTRDFLGLKPLSDSDILTIAGMGSCMNSSNSDHQENHSQKPWEGN