AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85217

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85217.t1 MTR_2g099990 95.949 469 13 4 1 464 1 468 0.0 926
MS.gene85217.t1 MTR_2g099990 95.750 400 12 3 69 464 1 399 0.0 795
MS.gene85217.t1 MTR_8g017210 57.957 509 127 18 1 446 18 502 2.90e-168 484
MS.gene85217.t1 MTR_1g016010 49.778 450 169 14 38 461 34 452 1.44e-129 383
MS.gene85217.t1 MTR_1g094115 88.415 164 19 0 42 205 44 207 3.76e-110 336
MS.gene85217.t1 MTR_4g059870 42.589 479 191 15 42 456 35 493 6.83e-109 331
MS.gene85217.t1 MTR_1g112270 88.125 160 19 0 48 207 47 206 2.26e-107 330
MS.gene85217.t1 MTR_1g112270 84.337 166 26 0 42 207 52 217 4.59e-104 322
MS.gene85217.t1 MTR_2g093960 81.928 166 29 1 44 208 31 196 4.11e-102 311
MS.gene85217.t1 MTR_2g090745 87.582 153 19 0 55 207 17 169 4.91e-101 309
MS.gene85217.t1 MTR_1g094115 78.049 164 19 1 42 205 44 190 4.25e-93 291
MS.gene85217.t1 MTR_2g017720 60.606 165 51 2 14 169 273 432 1.22e-59 202
MS.gene85217.t1 MTR_3g102000 70.707 99 22 2 72 166 36 131 2.26e-41 147
MS.gene85217.t1 MTR_5g030560 76.000 50 12 0 50 99 11 60 1.23e-21 89.0
MS.gene85217.t1 MTR_1g093095 35.172 145 79 6 66 197 107 249 2.17e-18 85.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85217.t1 AT1G55110 49.894 473 167 15 1 451 21 445 2.46e-128 380
MS.gene85217.t1 AT1G55110 49.894 473 167 15 1 451 21 445 2.46e-128 380
MS.gene85217.t1 AT1G55110 49.894 473 167 15 1 451 21 445 2.46e-128 380
MS.gene85217.t1 AT3G13810 76.364 220 39 3 1 210 20 236 5.76e-116 350
MS.gene85217.t1 AT3G13810 37.356 174 85 8 296 463 358 513 3.15e-18 87.8
MS.gene85217.t1 AT3G13810 76.364 220 39 3 1 210 20 236 5.76e-116 350
MS.gene85217.t1 AT3G13810 37.356 174 85 8 296 463 358 513 3.15e-18 87.8
MS.gene85217.t1 AT3G13810 76.364 220 39 3 1 210 1 217 7.36e-116 349
MS.gene85217.t1 AT3G13810 37.356 174 85 8 296 463 339 494 3.08e-18 87.4
MS.gene85217.t1 AT3G13810 76.364 220 39 3 1 210 15 231 8.69e-116 350
MS.gene85217.t1 AT3G13810 37.356 174 85 8 296 463 353 508 3.24e-18 87.4
MS.gene85217.t1 AT5G66730 73.430 207 38 1 1 207 5 194 5.70e-112 339
MS.gene85217.t1 AT3G50700 78.610 187 30 1 44 230 33 209 7.03e-112 338
MS.gene85217.t1 AT1G03840 88.415 164 19 0 44 207 40 203 8.28e-112 339
MS.gene85217.t1 AT5G44160 82.222 180 27 1 33 207 20 199 2.38e-109 332
MS.gene85217.t1 AT4G02670 51.093 366 127 9 10 369 19 338 1.95e-108 327
MS.gene85217.t1 AT4G02670 51.093 366 127 9 10 369 17 336 1.99e-108 327
MS.gene85217.t1 AT1G14580 71.078 204 54 1 4 207 17 215 2.08e-108 329
MS.gene85217.t1 AT1G14580 71.078 204 54 1 4 207 17 215 2.08e-108 329
MS.gene85217.t1 AT1G14580 71.078 204 54 1 4 207 31 229 4.69e-108 329
MS.gene85217.t1 AT2G02080 83.030 165 28 0 43 207 52 216 5.07e-108 330
MS.gene85217.t1 AT2G02080 83.030 165 28 0 43 207 52 216 5.07e-108 330
MS.gene85217.t1 AT2G02080 83.030 165 28 0 43 207 52 216 5.07e-108 330
MS.gene85217.t1 AT2G02080 83.030 165 28 0 43 207 52 216 5.07e-108 330
MS.gene85217.t1 AT1G03840 87.195 164 19 1 44 207 40 201 5.20e-108 329
MS.gene85217.t1 AT3G45260 80.769 182 32 2 44 222 38 219 1.86e-107 326
MS.gene85217.t1 AT3G45260 80.769 182 32 2 44 222 38 219 1.86e-107 326
MS.gene85217.t1 AT5G60470 76.316 190 42 3 31 218 31 219 4.63e-106 323
MS.gene85217.t1 AT5G60470 76.316 190 42 3 31 218 27 215 5.03e-106 323
MS.gene85217.t1 AT2G02070 80.682 176 33 1 45 220 52 226 5.97e-105 325
MS.gene85217.t1 AT2G02070 80.682 176 33 1 45 220 52 226 5.97e-105 325
MS.gene85217.t1 AT3G13810 69.912 226 49 3 1 210 1 223 1.86e-104 320
MS.gene85217.t1 AT3G13810 37.356 174 85 8 296 463 345 500 3.51e-18 87.4
MS.gene85217.t1 AT3G13810 69.912 226 49 3 1 210 15 237 2.50e-104 320
MS.gene85217.t1 AT3G13810 37.356 174 85 8 296 463 359 514 4.25e-18 87.0
MS.gene85217.t1 AT5G03150 84.242 165 25 1 44 207 52 216 2.57e-103 317
MS.gene85217.t1 AT5G44160 54.045 309 88 7 55 315 8 310 1.13e-100 308
MS.gene85217.t1 AT5G60470 79.394 165 32 2 56 218 46 210 6.05e-97 299
MS.gene85217.t1 AT2G02080 83.453 139 23 0 69 207 1 139 4.14e-91 284
MS.gene85217.t1 AT2G02080 83.453 139 23 0 69 207 1 139 4.14e-91 284
MS.gene85217.t1 AT1G68130 64.103 156 54 2 45 198 41 196 1.82e-72 235
MS.gene85217.t1 AT2G01940 60.345 174 61 3 45 216 44 211 3.31e-72 235
MS.gene85217.t1 AT1G25250 58.011 181 62 4 45 222 33 202 8.09e-72 232
MS.gene85217.t1 AT2G01940 59.429 175 62 4 45 216 44 212 1.28e-68 226
MS.gene85217.t1 AT1G25250 58.382 173 58 4 55 224 20 181 1.79e-68 223
MS.gene85217.t1 AT1G68130 64.545 110 37 2 91 198 1 110 6.55e-46 162
MS.gene85217.t1 AT2G01940 58.594 128 45 3 91 216 1 122 1.20e-45 162
MS.gene85217.t1 AT1G25250 55.474 137 47 4 91 224 1 126 2.13e-45 160
MS.gene85217.t1 AT1G34370 31.308 214 124 6 40 235 3 211 5.14e-24 102
MS.gene85217.t1 AT1G34370 30.876 217 127 6 37 235 210 421 1.05e-23 103
MS.gene85217.t1 AT1G34370 30.876 217 127 6 37 235 210 421 1.05e-23 103
MS.gene85217.t1 AT5G22890 32.026 153 90 3 59 197 202 354 2.01e-21 95.9
MS.gene85217.t1 AT1G13290 34.161 161 91 7 57 205 85 242 5.32e-19 87.8
MS.gene85217.t1 AT3G20880 33.562 146 87 4 61 197 243 387 2.66e-18 87.0
MS.gene85217.t1 AT1G51220 32.192 146 89 4 61 197 165 309 6.94e-18 85.1
MS.gene85217.t1 AT3G57670 32.653 147 87 5 61 197 204 348 1.17e-17 85.1
MS.gene85217.t1 AT1G08290 36.765 136 73 6 73 197 179 312 9.50e-17 81.6
MS.gene85217.t1 AT1G34790 32.450 151 90 5 57 197 128 276 4.36e-16 79.3

Find 96 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCTTCTTCTGGATTTCA+TGG 0.185525 2.4:-4343111 MS.gene85217:CDS
TTCTCTTGTGCCACAGGTTT+TGG 0.189840 2.4:+4344206 None:intergenic
AACAACTCTTTCTCTTCTTC+TGG 0.209031 2.4:-4343120 MS.gene85217:CDS
TTCACGTTGTGATGAGAATT+TGG 0.265319 2.4:+4343561 None:intergenic
GGTATTTGTACTTCTTGTTC+TGG 0.297642 2.4:+4343468 None:intergenic
GAAGCTAGTGCTAATTCTTC+TGG 0.298581 2.4:-4345128 MS.gene85217:CDS
GGAAGTGTAGGACCATTTCT+AGG 0.313383 2.4:+4343360 None:intergenic
ATCTCTCTTCAAACTATTCA+AGG 0.324026 2.4:-4343419 MS.gene85217:CDS
TTTGTTGCCATCAGTGTCTT+TGG 0.324056 2.4:+4344519 None:intergenic
AGCCTTGGATATCTTGAGTT+TGG 0.330060 2.4:+4343532 None:intergenic
GCACTTACAAGAGATTTCTT+AGG 0.351186 2.4:-4342952 MS.gene85217:CDS
AGAAAGAGTTGTTTATCATT+TGG 0.355557 2.4:+4343130 None:intergenic
GTAGGACCATTTCTAGGGTT+AGG 0.377918 2.4:+4343366 None:intergenic
ATCAAAGTTTCAAAATGTTA+AGG 0.407806 2.4:-4343491 MS.gene85217:CDS
AGTGATATCCTCACCATTGC+TGG 0.409995 2.4:-4342910 MS.gene85217:CDS
TCTAATTTGACTTCTGCATC+TGG 0.412009 2.4:-4345152 MS.gene85217:CDS
TAGGACCATTTCTAGGGTTA+GGG 0.414611 2.4:+4343367 None:intergenic
TGGTGCACACAAGTTGGTTC+TGG 0.417382 2.4:+4344360 None:intergenic
TATCCTCACCATTGCTGGTA+TGG 0.421802 2.4:-4342905 MS.gene85217:CDS
CATGACCCATCAAGAGCCTT+GGG 0.426349 2.4:-4344339 MS.gene85217:CDS
GTTGAGTGGGACCCACCAAC+AGG 0.427753 2.4:-4343238 MS.gene85217:CDS
CTTTCTTCAATGTGAAGCCT+TGG 0.431323 2.4:+4343517 None:intergenic
GTTCTTTGCTTCAACTTCCA+TGG 0.431659 2.4:+4344417 None:intergenic
TCTGTATTCTCTTGTGCCAC+AGG 0.435175 2.4:+4344200 None:intergenic
AAGAAAAGAAGAAACCTTCC+TGG 0.442018 2.4:-4345023 MS.gene85217:CDS
ATTTGAGTTTATCATGTTGT+TGG 0.445102 2.4:+4343188 None:intergenic
AAGCTATAACTTCAGCTTGT+GGG 0.447498 2.4:+4344547 None:intergenic
GAAAACCCTAACCCTAGAAA+TGG 0.454115 2.4:-4343372 MS.gene85217:CDS
AGTGCTTCTTGATACCAGTT+AGG 0.454887 2.4:+4344313 None:intergenic
TGATGAGAATTTGGCTGTGT+AGG 0.455107 2.4:+4343570 None:intergenic
GAAAATGAACCTGGATTGCC+AGG 0.461039 2.4:+4345005 None:intergenic
CTCTTCAAACTATTCAAGGT+TGG 0.466017 2.4:-4343415 MS.gene85217:CDS
TGCATTGTTTCCTCCGGCAC+CGG 0.471541 2.4:+4342972 None:intergenic
AGAAACCTTCCTGGCAATCC+AGG 0.472577 2.4:-4345014 MS.gene85217:intron
CCAAGGCTCTTGATGGGTCA+TGG 0.475409 2.4:+4344340 None:intergenic
TGTAAGTGCATTGTTTCCTC+CGG 0.475628 2.4:+4342966 None:intergenic
ATAGTTTGAAGAGAGATCAA+TGG 0.482584 2.4:+4343425 None:intergenic
CCATGACCCATCAAGAGCCT+TGG 0.490603 2.4:-4344340 MS.gene85217:CDS
ACCGGCACCGTCATCATCAT+TGG 0.490777 2.4:+4342990 None:intergenic
AACAACATGATAAACTCAAA+TGG 0.493838 2.4:-4343186 MS.gene85217:CDS
AATCTTCAACTTCATAAAAG+AGG 0.500458 2.4:-4344450 MS.gene85217:CDS
ATCCTCACCATTGCTGGTAT+GGG 0.502220 2.4:-4342904 MS.gene85217:CDS
AGTTGATGCAAAATTCTCTC+AGG 0.503692 2.4:-4345093 MS.gene85217:CDS
ATAGTTATACCTTGAGAAGA+GGG 0.512121 2.4:+4344164 None:intergenic
GATGATGACGGTGCCGGTGC+CGG 0.516820 2.4:-4342985 MS.gene85217:CDS
GTTACAGAAAGCAGCTCAGA+TGG 0.517581 2.4:-4343286 MS.gene85217:CDS
ATGAACCTGGATTGCCAGGA+AGG 0.520133 2.4:+4345009 None:intergenic
ATATGTGAGATCTGTAACAA+AGG 0.526520 2.4:-4344489 MS.gene85217:CDS
AAGAAGTACAAATACCCTCT+TGG 0.528888 2.4:-4343461 MS.gene85217:CDS
AGTATGCAGTTCAATCAGAC+TGG 0.532991 2.4:-4344239 MS.gene85217:CDS
AAAGAACAAGCAATGAGATA+AGG 0.533182 2.4:-4344401 MS.gene85217:CDS
TGAGTTTATCATGTTGTTGG+TGG 0.541113 2.4:+4343191 None:intergenic
GCAGATGATGGTTGGTAGGA+TGG 0.545269 2.4:+4343339 None:intergenic
TTGGTGGGTCCCACTCAACA+TGG 0.546559 2.4:+4343242 None:intergenic
CCAAACTAATTACCTTCCCA+TGG 0.548476 2.4:+4342829 None:intergenic
CAACATGGACTGAGATGAGC+TGG 0.548999 2.4:+4343257 None:intergenic
TATCATTTGGTCTTCACATG+AGG 0.559455 2.4:+4343143 None:intergenic
TATAGTTATACCTTGAGAAG+AGG 0.562344 2.4:+4344163 None:intergenic
GAACATAATATCAATCATGA+TGG 0.565329 2.4:-4343039 MS.gene85217:CDS
GAAAGCACGGTGAGAAGAAG+TGG 0.566601 2.4:-4344284 MS.gene85217:CDS
GAAGTGTAGGACCATTTCTA+GGG 0.573649 2.4:+4343361 None:intergenic
TCATCTCAGTCCATGTTGAG+TGG 0.578988 2.4:-4343252 MS.gene85217:CDS
TGAGGTGAAGCTGCAGATGA+TGG 0.582967 2.4:+4343327 None:intergenic
TAGACACGTGACCCTGTTGG+TGG 0.583962 2.4:+4343226 None:intergenic
GGCACCGTCATCATCATTGG+TGG 0.585980 2.4:+4342993 None:intergenic
AAAGAGGACACAATTTGCCA+TGG 0.587343 2.4:-4344434 MS.gene85217:CDS
CATCTCAGTCCATGTTGAGT+GGG 0.587878 2.4:-4343251 MS.gene85217:CDS
CTCAGCTAATGCATCACAGA+AGG 0.590439 2.4:+4343620 None:intergenic
CTCCAAACTCAAGATATCCA+AGG 0.596098 2.4:-4343534 MS.gene85217:CDS
GCAACTTCCCATACCAGCAA+TGG 0.602937 2.4:+4342897 None:intergenic
AAAGCTATAACTTCAGCTTG+TGG 0.603171 2.4:+4344546 None:intergenic
AAGCACTTCTGCAGAAAGCA+CGG 0.603251 2.4:-4344297 MS.gene85217:CDS
GATGATTGACAAAGCCAAGA+GGG 0.607399 2.4:+4343447 None:intergenic
GAATTTGGCTGTGTAGGTTG+AGG 0.609498 2.4:+4343576 None:intergenic
GATGACGGTGCCGGTGCCGG+AGG 0.615538 2.4:-4342982 MS.gene85217:CDS
TTCCCATACCAGCAATGGTG+AGG 0.621943 2.4:+4342902 None:intergenic
CAATAGACACGTGACCCTGT+TGG 0.624582 2.4:+4343223 None:intergenic
ACCAATGATGATGACGGTGC+CGG 0.625084 2.4:-4342991 MS.gene85217:CDS
ACTGTGGAACCCTCTTCTCA+AGG 0.626939 2.4:-4344173 MS.gene85217:intron
TCAACCACCAATGATGATGA+CGG 0.633146 2.4:-4342997 MS.gene85217:CDS
TTACAGAAAGCAGCTCAGAT+GGG 0.645481 2.4:-4343285 MS.gene85217:CDS
GGTTGGTAGGATGGAAGTGT+AGG 0.650505 2.4:+4343348 None:intergenic
AGACACGTGACCCTGTTGGT+GGG 0.651658 2.4:+4343227 None:intergenic
TGACCCATCAAGAGCCTTGG+GGG 0.652313 2.4:-4344337 MS.gene85217:CDS
GTGAAGCTGCAGATGATGGT+TGG 0.652944 2.4:+4343331 None:intergenic
TTTGTCACCAAAGACACTGA+TGG 0.656547 2.4:-4344526 MS.gene85217:CDS
AGGATATCACTATCAGAGAG+AGG 0.661126 2.4:+4342922 None:intergenic
AGCTGCAGATGATGGTTGGT+AGG 0.662572 2.4:+4343335 None:intergenic
CTTCTGTGATGCATTAGCTG+AGG 0.666815 2.4:-4343619 MS.gene85217:CDS
GGGTCATGGTGCACACAAGT+TGG 0.674016 2.4:+4344354 None:intergenic
TTGAGTGGGACCCACCAACA+GGG 0.675919 2.4:-4343237 MS.gene85217:CDS
AAAGCTCACTCCAAAACCTG+TGG 0.698299 2.4:-4344216 MS.gene85217:CDS
AGAGAATACAGATGTGACTG+TGG 0.700052 2.4:-4344189 MS.gene85217:CDS
GGATGATTGACAAAGCCAAG+AGG 0.702136 2.4:+4343446 None:intergenic
ATGACCCATCAAGAGCCTTG+GGG 0.705499 2.4:-4344338 MS.gene85217:CDS
AATGCTGTAGCTGACATGTG+AGG 0.760209 2.4:+4343309 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATATATTATATGATTTTC+AGG - chr2.4:4344321-4344340 MS.gene85217:CDS 10.0%
!!! ATTATTTTATTAAACTGTTT+AGG + chr2.4:4343866-4343885 None:intergenic 10.0%
!! ATCTTAATTGTTATAAATTG+TGG - chr2.4:4343032-4343051 MS.gene85217:CDS 15.0%
!!! CATATTTTGTTTATCTAAAT+TGG - chr2.4:4343371-4343390 MS.gene85217:CDS 15.0%
!! ATCAAAGTTTCAAAATGTTA+AGG - chr2.4:4344498-4344517 MS.gene85217:CDS 20.0%
!! ATTAAGATGAAAAGAAAAGA+TGG + chr2.4:4343020-4343039 None:intergenic 20.0%
!! GAAAAATATGAAGAAAAACA+TGG - chr2.4:4343098-4343117 MS.gene85217:CDS 20.0%
!! TATACTATTATCAACTTGAA+GGG + chr2.4:4343904-4343923 None:intergenic 20.0%
!!! ATATTATATGATTTTCAGGA+GGG - chr2.4:4344325-4344344 MS.gene85217:CDS 20.0%
!!! CAAACATTTTTTAAAGAAAG+TGG - chr2.4:4343955-4343974 MS.gene85217:intron 20.0%
!!! GAAAGTGGAAATTTTTAAAA+TGG - chr2.4:4343970-4343989 MS.gene85217:intron 20.0%
!!! TATATTATATGATTTTCAGG+AGG - chr2.4:4344324-4344343 MS.gene85217:CDS 20.0%
! AACAACATGATAAACTCAAA+TGG - chr2.4:4344803-4344822 MS.gene85217:intron 25.0%
! AATCTTCAACTTCATAAAAG+AGG - chr2.4:4343539-4343558 MS.gene85217:CDS 25.0%
! ACTTAGTAATCTCATGTATA+AGG - chr2.4:4344178-4344197 MS.gene85217:CDS 25.0%
! AGAAAGAGTTGTTTATCATT+TGG + chr2.4:4344862-4344881 None:intergenic 25.0%
! GAACATAATATCAATCATGA+TGG - chr2.4:4344950-4344969 MS.gene85217:intron 25.0%
! GTATACTATTATCAACTTGA+AGG + chr2.4:4343905-4343924 None:intergenic 25.0%
! TTTAACATGTTATGCTTGTT+TGG - chr2.4:4343219-4343238 MS.gene85217:CDS 25.0%
!! ATTTGAGTTTATCATGTTGT+TGG + chr2.4:4344804-4344823 None:intergenic 25.0%
!! CATATTTTTCCACAATATCA+TGG + chr2.4:4343088-4343107 None:intergenic 25.0%
!! TTGGATAAATGTGAATTTTC+CGG + chr2.4:4344153-4344172 None:intergenic 25.0%
AAAGAACAAGCAATGAGATA+AGG - chr2.4:4343588-4343607 MS.gene85217:CDS 30.0%
ACTTGAAGGGATAAAAAATG+TGG + chr2.4:4343891-4343910 None:intergenic 30.0%
AGAACTTAAGAAAATGAACC+TGG + chr2.4:4342996-4343015 None:intergenic 30.0%
ATAAATTGTGGTTATAGTCG+CGG - chr2.4:4343044-4343063 MS.gene85217:CDS 30.0%
ATAGTTATACCTTGAGAAGA+GGG + chr2.4:4343828-4343847 None:intergenic 30.0%
ATAGTTTGAAGAGAGATCAA+TGG + chr2.4:4344567-4344586 None:intergenic 30.0%
ATATGTGAGATCTGTAACAA+AGG - chr2.4:4343500-4343519 MS.gene85217:CDS 30.0%
ATCTCTCTTCAAACTATTCA+AGG - chr2.4:4344570-4344589 MS.gene85217:intron 30.0%
ATTGTGAATCAGAAAATGAG+AGG - chr2.4:4344221-4344240 MS.gene85217:CDS 30.0%
CAACAAAATCATACTAACAC+CGG - chr2.4:4344131-4344150 MS.gene85217:intron 30.0%
GCAATGACATCTAATTAAAC+CGG - chr2.4:4343155-4343174 MS.gene85217:CDS 30.0%
TATAGTTATACCTTGAGAAG+AGG + chr2.4:4343829-4343848 None:intergenic 30.0%
TTAATTAGATGTCATTGCAG+TGG + chr2.4:4343153-4343172 None:intergenic 30.0%
TTGTGAATCAGAAAATGAGA+GGG - chr2.4:4344222-4344241 MS.gene85217:CDS 30.0%
! ATTTCATGGAACTTCTTTTG+AGG - chr2.4:4344892-4344911 MS.gene85217:intron 30.0%
! TTTTTAATGTACGATCTCTG+TGG - chr2.4:4344004-4344023 MS.gene85217:intron 30.0%
AAAACCATTCCCAAAAACCA+TGG - chr2.4:4345143-4345162 MS.gene85217:CDS 35.0%
AAACCATTCCCAAAAACCAT+GGG - chr2.4:4345144-4345163 MS.gene85217:CDS 35.0%
AAAGCTATAACTTCAGCTTG+TGG + chr2.4:4343446-4343465 None:intergenic 35.0%
AACAACTCTTTCTCTTCTTC+TGG - chr2.4:4344869-4344888 MS.gene85217:intron 35.0%
AAGAAAAGAAGAAACCTTCC+TGG - chr2.4:4342966-4342985 MS.gene85217:CDS 35.0%
AAGCTATAACTTCAGCTTGT+GGG + chr2.4:4343445-4343464 None:intergenic 35.0%
ATAGTGCATGCTTAGATTGA+TGG - chr2.4:4344037-4344056 MS.gene85217:intron 35.0%
CTCTTCAAACTATTCAAGGT+TGG - chr2.4:4344574-4344593 MS.gene85217:intron 35.0%
GCACTTACAAGAGATTTCTT+AGG - chr2.4:4345037-4345056 MS.gene85217:CDS 35.0%
GGTATTTGTACTTCTTGTTC+TGG + chr2.4:4344524-4344543 None:intergenic 35.0%
TGAGATTACTAAGTGCATGT+TGG + chr2.4:4344172-4344191 None:intergenic 35.0%
TGAGTTTATCATGTTGTTGG+TGG + chr2.4:4344801-4344820 None:intergenic 35.0%
TTCACGTTGTGATGAGAATT+TGG + chr2.4:4344431-4344450 None:intergenic 35.0%
TTCTCTTCTTCTGGATTTCA+TGG - chr2.4:4344878-4344897 MS.gene85217:intron 35.0%
! AAGAAGTACAAATACCCTCT+TGG - chr2.4:4344528-4344547 MS.gene85217:CDS 35.0%
! AATTACCTTCCCATGGTTTT+TGG + chr2.4:4345156-4345175 None:intergenic 35.0%
! AGGTTTCTTCTTTTCTTAGC+TGG + chr2.4:4342963-4342982 None:intergenic 35.0%
! AGTTGATGCAAAATTCTCTC+AGG - chr2.4:4342896-4342915 MS.gene85217:CDS 35.0%
! ATTACCTTCCCATGGTTTTT+GGG + chr2.4:4345155-4345174 None:intergenic 35.0%
! GAAGAAAAACATGGTTGATG+CGG - chr2.4:4343107-4343126 MS.gene85217:CDS 35.0%
! GTTATGCTTGTTTGGAATCA+TGG - chr2.4:4343227-4343246 MS.gene85217:CDS 35.0%
! TAGGGTTAGGGTTTTCATAA+AGG + chr2.4:4344613-4344632 None:intergenic 35.0%
! TATCATTTGGTCTTCACATG+AGG + chr2.4:4344849-4344868 None:intergenic 35.0%
! TCTAATTTGACTTCTGCATC+TGG - chr2.4:4342837-4342856 MS.gene85217:CDS 35.0%
!! GGAATGGTTTTCTTGATGAT+CGG + chr2.4:4345134-4345153 None:intergenic 35.0%
!! TCTTTTGAGGATACTTTTGC+TGG - chr2.4:4344905-4344924 MS.gene85217:intron 35.0%
AAAGAGGACACAATTTGCCA+TGG - chr2.4:4343555-4343574 MS.gene85217:CDS 40.0%
AATATGAATGCGCGCATGTT+TGG + chr2.4:4343357-4343376 None:intergenic 40.0%
AGAGAATACAGATGTGACTG+TGG - chr2.4:4343800-4343819 MS.gene85217:intron 40.0%
AGCCTTGGATATCTTGAGTT+TGG + chr2.4:4344460-4344479 None:intergenic 40.0%
AGGATATCACTATCAGAGAG+AGG + chr2.4:4345070-4345089 None:intergenic 40.0%
AGTATGCAGTTCAATCAGAC+TGG - chr2.4:4343750-4343769 MS.gene85217:intron 40.0%
AGTGCTTCTTGATACCAGTT+AGG + chr2.4:4343679-4343698 None:intergenic 40.0%
CGCTATGAACCATGATATTG+TGG - chr2.4:4343076-4343095 MS.gene85217:CDS 40.0%
CTCCAAACTCAAGATATCCA+AGG - chr2.4:4344455-4344474 MS.gene85217:CDS 40.0%
CTCGAGCTTCAACAAAATCA+CGG + chr2.4:4343275-4343294 None:intergenic 40.0%
CTTTCTTCAATGTGAAGCCT+TGG + chr2.4:4344475-4344494 None:intergenic 40.0%
GAAAACCCTAACCCTAGAAA+TGG - chr2.4:4344617-4344636 MS.gene85217:intron 40.0%
GAAGCTAGTGCTAATTCTTC+TGG - chr2.4:4342861-4342880 MS.gene85217:CDS 40.0%
GAAGTGTAGGACCATTTCTA+GGG + chr2.4:4344631-4344650 None:intergenic 40.0%
GATGATTGACAAAGCCAAGA+GGG + chr2.4:4344545-4344564 None:intergenic 40.0%
GTTCTTTGCTTCAACTTCCA+TGG + chr2.4:4343575-4343594 None:intergenic 40.0%
TGATGAGAATTTGGCTGTGT+AGG + chr2.4:4344422-4344441 None:intergenic 40.0%
TGGCATCACAACTACAATTG+CGG + chr2.4:4343133-4343152 None:intergenic 40.0%
TGTAAGTGCATTGTTTCCTC+CGG + chr2.4:4345026-4345045 None:intergenic 40.0%
TGTACGATCTCTGTGGTTAT+CGG - chr2.4:4344011-4344030 MS.gene85217:intron 40.0%
TTACAGAAAGCAGCTCAGAT+GGG - chr2.4:4344704-4344723 MS.gene85217:intron 40.0%
TTTGTCACCAAAGACACTGA+TGG - chr2.4:4343463-4343482 MS.gene85217:CDS 40.0%
TTTGTTGCCATCAGTGTCTT+TGG + chr2.4:4343473-4343492 None:intergenic 40.0%
! TCAACCACCAATGATGATGA+CGG - chr2.4:4344992-4345011 MS.gene85217:intron 40.0%
!! GTTTTCTTGATGATCGGAGT+TGG + chr2.4:4345128-4345147 None:intergenic 40.0%
!! TAGGACCATTTCTAGGGTTA+GGG + chr2.4:4344625-4344644 None:intergenic 40.0%
AAAGCTCACTCCAAAACCTG+TGG - chr2.4:4343773-4343792 MS.gene85217:intron 45.0%
AAGCACTTCTGCAGAAAGCA+CGG - chr2.4:4343692-4343711 MS.gene85217:intron 45.0%
AATGCTGTAGCTGACATGTG+AGG + chr2.4:4344683-4344702 None:intergenic 45.0%
AGTGATATCCTCACCATTGC+TGG - chr2.4:4345079-4345098 MS.gene85217:CDS 45.0%
ATCCTCACCATTGCTGGTAT+GGG - chr2.4:4345085-4345104 MS.gene85217:CDS 45.0%
CATCTCAGTCCATGTTGAGT+GGG - chr2.4:4344738-4344757 MS.gene85217:intron 45.0%
CATTCCCAAAAACCATGGGA+AGG - chr2.4:4345148-4345167 MS.gene85217:CDS 45.0%
CGTTTGCAGCAGCAATATAC+CGG + chr2.4:4343177-4343196 None:intergenic 45.0%
CTCAGCTAATGCATCACAGA+AGG + chr2.4:4344372-4344391 None:intergenic 45.0%
CTTCTGTGATGCATTAGCTG+AGG - chr2.4:4344370-4344389 MS.gene85217:CDS 45.0%
GAAAATGAACCTGGATTGCC+AGG + chr2.4:4342987-4343006 None:intergenic 45.0%
GAATTTGGCTGTGTAGGTTG+AGG + chr2.4:4344416-4344435 None:intergenic 45.0%
GAGCTTCAACAAAATCACGG+TGG + chr2.4:4343272-4343291 None:intergenic 45.0%
GGATGATTGACAAAGCCAAG+AGG + chr2.4:4344546-4344565 None:intergenic 45.0%
GTTACAGAAAGCAGCTCAGA+TGG - chr2.4:4344703-4344722 MS.gene85217:intron 45.0%
TATCCTCACCATTGCTGGTA+TGG - chr2.4:4345084-4345103 MS.gene85217:CDS 45.0%
TCATCTCAGTCCATGTTGAG+TGG - chr2.4:4344737-4344756 MS.gene85217:intron 45.0%
TCTGTATTCTCTTGTGCCAC+AGG + chr2.4:4343792-4343811 None:intergenic 45.0%
! GGAAGTGTAGGACCATTTCT+AGG + chr2.4:4344632-4344651 None:intergenic 45.0%
! TTCTCTTGTGCCACAGGTTT+TGG + chr2.4:4343786-4343805 None:intergenic 45.0%
!! CTTCCCATGGTTTTTGGGAA+TGG + chr2.4:4345150-4345169 None:intergenic 45.0%
!! GTAGGACCATTTCTAGGGTT+AGG + chr2.4:4344626-4344645 None:intergenic 45.0%
ACTGTGGAACCCTCTTCTCA+AGG - chr2.4:4343816-4343835 MS.gene85217:intron 50.0%
AGAAACCTTCCTGGCAATCC+AGG - chr2.4:4342975-4342994 MS.gene85217:CDS 50.0%
ATGAACCTGGATTGCCAGGA+AGG + chr2.4:4342983-4343002 None:intergenic 50.0%
ATGACCCATCAAGAGCCTTG+GGG - chr2.4:4343651-4343670 MS.gene85217:intron 50.0%
CAATAGACACGTGACCCTGT+TGG + chr2.4:4344769-4344788 None:intergenic 50.0%
CATGACCCATCAAGAGCCTT+GGG - chr2.4:4343650-4343669 MS.gene85217:intron 50.0%
GCAACTTCCCATACCAGCAA+TGG + chr2.4:4345095-4345114 None:intergenic 50.0%
GCGTAGCTTCTGCAAACTCA+CGG - chr2.4:4343296-4343315 MS.gene85217:CDS 50.0%
TGAGGTGAAGCTGCAGATGA+TGG + chr2.4:4344665-4344684 None:intergenic 50.0%
TTCCCATACCAGCAATGGTG+AGG + chr2.4:4345090-4345109 None:intergenic 50.0%
! AGCTGCAGATGATGGTTGGT+AGG + chr2.4:4344657-4344676 None:intergenic 50.0%
! CAACATGGACTGAGATGAGC+TGG + chr2.4:4344735-4344754 None:intergenic 50.0%
! GAAAGCACGGTGAGAAGAAG+TGG - chr2.4:4343705-4343724 MS.gene85217:intron 50.0%
! GTGAAGCTGCAGATGATGGT+TGG + chr2.4:4344661-4344680 None:intergenic 50.0%
!! ACCAATGATGATGACGGTGC+CGG - chr2.4:4344998-4345017 MS.gene85217:intron 50.0%
!! GCAGATGATGGTTGGTAGGA+TGG + chr2.4:4344653-4344672 None:intergenic 50.0%
!! GGTTGGTAGGATGGAAGTGT+AGG + chr2.4:4344644-4344663 None:intergenic 50.0%
!! TGGTGCACACAAGTTGGTTC+TGG + chr2.4:4343632-4343651 None:intergenic 50.0%
ACCGGCACCGTCATCATCAT+TGG + chr2.4:4345002-4345021 None:intergenic 55.0%
AGACACGTGACCCTGTTGGT+GGG + chr2.4:4344765-4344784 None:intergenic 55.0%
CCATGACCCATCAAGAGCCT+TGG - chr2.4:4343649-4343668 MS.gene85217:CDS 55.0%
GATACCAGTTAGGTCCCCCA+AGG + chr2.4:4343669-4343688 None:intergenic 55.0%
GGCACCGTCATCATCATTGG+TGG + chr2.4:4344999-4345018 None:intergenic 55.0%
GGGTCATGGTGCACACAAGT+TGG + chr2.4:4343638-4343657 None:intergenic 55.0%
TAGACACGTGACCCTGTTGG+TGG + chr2.4:4344766-4344785 None:intergenic 55.0%
TGACCCATCAAGAGCCTTGG+GGG - chr2.4:4343652-4343671 MS.gene85217:intron 55.0%
TGCATTGTTTCCTCCGGCAC+CGG + chr2.4:4345020-4345039 None:intergenic 55.0%
TTGAGTGGGACCCACCAACA+GGG - chr2.4:4344752-4344771 MS.gene85217:intron 55.0%
! CCAAGGCTCTTGATGGGTCA+TGG + chr2.4:4343652-4343671 None:intergenic 55.0%
! TTGGTGGGTCCCACTCAACA+TGG + chr2.4:4344750-4344769 None:intergenic 55.0%
AGAGCCTTGGGGGACCTAAC+TGG - chr2.4:4343662-4343681 MS.gene85217:intron 60.0%
AGGTCCCCCAAGGCTCTTGA+TGG + chr2.4:4343659-4343678 None:intergenic 60.0%
GGTCCCCCAAGGCTCTTGAT+GGG + chr2.4:4343658-4343677 None:intergenic 60.0%
GTTGAGTGGGACCCACCAAC+AGG - chr2.4:4344751-4344770 MS.gene85217:intron 60.0%
!! GATGATGACGGTGCCGGTGC+CGG - chr2.4:4345004-4345023 MS.gene85217:intron 65.0%
!! GATGACGGTGCCGGTGCCGG+AGG - chr2.4:4345007-4345026 MS.gene85217:intron 75.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 4342834 4345177 4342834 ID=MS.gene85217
chr2.4 mRNA 4342834 4345177 4342834 ID=MS.gene85217.t1;Parent=MS.gene85217
chr2.4 exon 4345015 4345177 4345015 ID=MS.gene85217.t1.exon1;Parent=MS.gene85217.t1
chr2.4 CDS 4345015 4345177 4345015 ID=cds.MS.gene85217.t1;Parent=MS.gene85217.t1
chr2.4 exon 4344174 4344570 4344174 ID=MS.gene85217.t1.exon2;Parent=MS.gene85217.t1
chr2.4 CDS 4344174 4344570 4344174 ID=cds.MS.gene85217.t1;Parent=MS.gene85217.t1
chr2.4 exon 4342834 4343668 4342834 ID=MS.gene85217.t1.exon3;Parent=MS.gene85217.t1
chr2.4 CDS 4342834 4343668 4342834 ID=cds.MS.gene85217.t1;Parent=MS.gene85217.t1
Gene Sequence

>MS.gene85217

ATGTCTAATTTGACTTCTGCATCTGGTGAAGCTAGTGCTAATTCTTCTGGAAACAGAACTGAAGTTGATGCAAAATTCTCTCAGGAATACTTTGCTTCATCACAAACTCAAACTCATGATGAGACTCCAGCTAAGAAAAGAAGAAACCTTCCTGGCAATCCAGGTTCATTTTCTTAAGTTCTACCATCTTTTCTTTTCATCTTAATTGTTATAAATTGTGGTTATAGTCGCGGTTATGATTACGCTATGAACCATGATATTGTGGAAAAATATGAAGAAAAACATGGTTGATGCGGCCGCAATTGTAGTTGTGATGCCACTGCAATGACATCTAATTAAACCGGTATATTGCTGCTGCAAACGACAATTTAGAACTATATGTGTATTTAACATGTTATGCTTGTTTGGAATCATGGCATAGATTTGACAAAGTTACCACCGTGATTTTGTTGAAGCTCGAGAGCGTAGCTTCTGCAAACTCACGGTGACTGACAGTAATTTTATCAAACTCATCGTGAATCCAAACATGCGCGCATTCATATTTTGTTTATCTAAATTGGATTTCTTTTGTTTTGTGTTTGTTTTTTTATTTGATTATTCATTTTAGACCCACAAGCTGAAGTTATAGCTTTGTCACCAAAGACACTGATGGCAACAAATAGATTCATATGTGAGATCTGTAACAAAGGATTTCAAAGAGATCAGAATCTTCAACTTCATAAAAGAGGACACAATTTGCCATGGAAGTTGAAGCAAAGAACAAGCAATGAGATAAGGAAGAAAGTGTATGTGTGTCCAGAACCAACTTGTGTGCACCATGACCCATCAAGAGCCTTGGGGGACCTAACTGGTATCAAGAAGCACTTCTGCAGAAAGCACGGTGAGAAGAAGTGGAAATGTGACAAGTGCTCTAAGAAGTATGCAGTTCAATCAGACTGGAAAGCTCACTCCAAAACCTGTGGCACAAGAGAATACAGATGTGACTGTGGAACCCTCTTCTCAAGGTATAACTATAAATATATTAACTAACCTAAACAGTTTAATAAAATAATTACCACATTTTTTATCCCTTCAAGTTGATAATAGTATACTGAACATGTAAAAAGAGTTCAATACATAGTCAAACATTTTTTAAAGAAAGTGGAAATTTTTAAAATGGAAAATTGAATTTTTTTAATGTACGATCTCTGTGGTTATCGGTGCATAGTGCATGCTTAGATTGATGGTGAATTTCTCAGAATCATTTAGAATCACATTGTATCATCTTAATTTTGACAAAAGTTATACTTCAGAGTTTCAACAAAATCATACTAACACCGGAAAATTCACATTTATCCAACATGCACTTAGTAATCTCATGTATAAGGTGAGTGAGACTAAAAAAATGATTGTGAATCAGAAAATGAGAGGGTGAATATTTCTTGACTGTTATAGCATTTTAGTAACATTGTTCACTTTTGTATTTTTGTGTTTGTTTATATATATATATATATATTATATGATTTTCAGGAGGGACAGTTTCATAACTCACAGAGCCTTCTGTGATGCATTAGCTGAGGAGAGTTCAAGAACTGTAATTCCTCAACCTACACAGCCAAATTCTCATCACAACGTGAACAATCTCCAAACTCAAGATATCCAAGGCTTCACATTGAAGAAAGAGCATCAAAGTTTCAAAATGTTAAGGCCAGAACAAGAAGTACAAATACCCTCTTGGCTTTGTCAATCATCCATTGATCTCTCTTCAAACTATTCAAGGTTGGATCAAGATCTACACCTTTATGAAAACCCTAACCCTAGAAATGGTCCTACACTTCCATCCTACCAACCATCATCTGCAGCTTCACCTCACATGTCAGCTACAGCATTGTTACAGAAAGCAGCTCAGATGGGTGCAACCAGCTCATCTCAGTCCATGTTGAGTGGGACCCACCAACAGGGTCACGTGTCTATTGTTGACTCTGCCACCAACAACATGATAAACTCAAATGGTAATTTTAGTTTGAATTTGTCCTCATGTGAAGACCAAATGATAAACAACTCTTTCTCTTCTTCTGGATTTCATGGAACTTCTTTTGAGGATACTTTTGCTGGAAATATTCTTCATTCAAATCAAGAACATAATATCAATCATGATGGTAATAATGATATTACAAAATCAACCACCAATGATGATGACGGTGCCGGTGCCGGAGGAAACAATGCACTTACAAGAGATTTCTTAGGTCTTAAACCTCTCTCTGATAGTGATATCCTCACCATTGCTGGTATGGGAAGTTGCATGAACTCTTCCAACTCCGATCATCAAGAAAACCATTCCCAAAAACCATGGGAAGGTAATTAG
Protein sequence

>MS.gene85217.t1

MSNLTSASGEASANSSGNRTEVDAKFSQEYFASSQTQTHDETPAKKRRNLPGNPDPQAEVIALSPKTLMATNRFICEICNKGFQRDQNLQLHKRGHNLPWKLKQRTSNEIRKKVYVCPEPTCVHHDPSRALGDLTGIKKHFCRKHGEKKWKCDKCSKKYAVQSDWKAHSKTCGTREYRCDCGTLFSRRDSFITHRAFCDALAEESSRTVIPQPTQPNSHHNVNNLQTQDIQGFTLKKEHQSFKMLRPEQEVQIPSWLCQSSIDLSSNYSRLDQDLHLYENPNPRNGPTLPSYQPSSAASPHMSATALLQKAAQMGATSSSQSMLSGTHQQGHVSIVDSATNNMINSNGNFSLNLSSCEDQMINNSFSSSGFHGTSFEDTFAGNILHSNQEHNINHDGNNDITKSTTNDDDGAGAGGNNALTRDFLGLKPLSDSDILTIAGMGSCMNSSNSDHQENHSQKPWEGN