AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene051976

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051976.t1 MTR_2g099490 94.524 347 17 1 1 345 1 347 0.0 653
MS.gene051976.t1 MTR_2g099490 91.014 345 17 1 1 345 1 331 0.0 621
MS.gene051976.t1 MTR_8g019540 51.176 340 121 12 23 345 7 318 1.50e-83 256
MS.gene051976.t1 MTR_2g041090 55.147 136 49 4 121 253 19 145 8.02e-40 140
MS.gene051976.t1 MTR_2g030170 52.174 115 51 2 168 282 95 205 4.95e-33 122
MS.gene051976.t1 MTR_2g030170 52.174 115 51 2 168 282 128 238 2.27e-32 121
MS.gene051976.t1 MTR_3g061510 47.020 151 65 3 96 246 94 229 8.91e-32 122
MS.gene051976.t1 MTR_3g061510 47.020 151 65 3 96 246 114 249 1.10e-31 122
MS.gene051976.t1 MTR_8g037270 56.311 103 40 1 144 246 117 214 1.20e-30 118
MS.gene051976.t1 MTR_7g106450 51.000 100 40 3 147 246 136 226 4.91e-24 100
MS.gene051976.t1 MTR_1g056530 41.844 141 67 4 108 245 95 223 6.21e-24 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051976.t1 AT1G17590 38.310 355 162 14 10 345 12 328 9.67e-52 174
MS.gene051976.t1 AT1G17590 38.310 355 162 14 10 345 12 328 9.67e-52 174
MS.gene051976.t1 AT1G17590 38.310 355 162 14 10 345 12 328 9.67e-52 174
MS.gene051976.t1 AT1G17590 38.310 355 162 14 10 345 12 328 9.67e-52 174
MS.gene051976.t1 AT1G72830 35.537 363 189 13 2 345 4 340 2.44e-48 166
MS.gene051976.t1 AT1G72830 35.440 364 189 14 2 345 4 341 1.65e-46 161
MS.gene051976.t1 AT3G14020 37.690 329 134 16 20 318 13 300 1.13e-39 142
MS.gene051976.t1 AT3G14020 37.690 329 134 16 20 318 13 300 1.13e-39 142
MS.gene051976.t1 AT1G54160 38.281 256 107 8 22 250 11 242 1.17e-39 142
MS.gene051976.t1 AT1G30500 51.938 129 58 2 130 254 40 168 1.02e-36 131
MS.gene051976.t1 AT1G72830 34.234 333 154 11 2 318 4 287 1.65e-36 134
MS.gene051976.t1 AT1G30500 52.381 126 58 2 130 254 40 164 1.85e-36 130
MS.gene051976.t1 AT2G34720 51.613 124 51 3 123 246 44 158 7.11e-33 121
MS.gene051976.t1 AT2G34720 52.941 119 47 3 128 246 14 123 1.52e-31 117
MS.gene051976.t1 AT2G34720 52.941 119 47 3 128 246 14 123 1.52e-31 117
MS.gene051976.t1 AT3G20910 45.926 135 59 4 117 251 111 231 1.45e-29 115
MS.gene051976.t1 AT5G12840 47.015 134 55 4 116 248 115 233 7.08e-29 112
MS.gene051976.t1 AT5G12840 47.015 134 55 4 116 248 115 233 7.08e-29 112
MS.gene051976.t1 AT5G12840 47.015 134 55 4 116 248 116 234 7.46e-29 112
MS.gene051976.t1 AT5G12840 47.015 134 55 4 116 248 116 234 7.46e-29 112
MS.gene051976.t1 AT5G12840 47.015 134 55 4 116 248 116 234 7.46e-29 112
MS.gene051976.t1 AT5G06510 48.214 112 46 4 149 259 9 109 5.24e-24 97.4
MS.gene051976.t1 AT5G06510 48.214 112 46 4 149 259 9 109 5.24e-24 97.4
MS.gene051976.t1 AT5G06510 47.788 113 47 4 149 260 104 205 1.40e-23 98.6
MS.gene051976.t1 AT5G06510 47.788 113 47 4 149 260 104 205 1.40e-23 98.6
MS.gene051976.t1 AT5G06510 47.788 113 47 4 149 260 104 205 1.40e-23 98.6
MS.gene051976.t1 AT5G06510 47.788 113 47 4 149 260 104 205 1.40e-23 98.6
MS.gene051976.t1 AT5G06510 47.788 113 47 4 149 260 55 156 2.55e-23 96.7
MS.gene051976.t1 AT3G05690 40.426 141 68 5 109 245 71 199 4.46e-20 89.4
MS.gene051976.t1 AT3G05690 40.426 141 68 5 109 245 108 236 5.63e-20 89.7

Find 79 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTTTGAATTCACATATA+TGG 0.180422 2.2:+3473861 None:intergenic
CACTTTGAAGCTCAAACTTT+TGG 0.192638 2.2:+3475550 None:intergenic
TTCAACCTGATCATGCATTT+TGG 0.229189 2.2:+3473506 None:intergenic
GCGAATCAAGCTTTGATTCT+TGG 0.254868 2.2:+3473595 None:intergenic
TTCATATGGCCAACAATATA+AGG 0.305273 2.2:-3474426 MS.gene051976:intron
TATAGCTGGTGGAAACATTT+TGG 0.306979 2.2:+3473562 None:intergenic
GAGAACCGAGGAGAACAATA+TGG 0.321909 2.2:-3474667 MS.gene051976:CDS
TCATCGAATCATCATCTATT+AGG 0.333409 2.2:-3474638 MS.gene051976:CDS
AAATTCTGACCAGCTTCTTC+AGG 0.334629 2.2:-3473944 MS.gene051976:CDS
TGCTTTGAATTCACATATAT+GGG 0.338735 2.2:+3473862 None:intergenic
TTCCAGCATTGTTCTTCTAC+TGG 0.343547 2.2:-3474702 MS.gene051976:CDS
GGAGGATGGACGGTGAGATT+TGG 0.358180 2.2:+3474612 None:intergenic
AATTCCCTTGCCATCTGATA+TGG 0.360890 2.2:-3473893 MS.gene051976:CDS
GCTTCTTCAGGGCCAGCTAA+TGG 0.362306 2.2:-3473932 MS.gene051976:CDS
GGCCTACTTTCTGCTTCATA+TGG 0.362322 2.2:-3474440 MS.gene051976:CDS
CACCGTCCATCCTCCACTAA+TGG 0.364359 2.2:-3474604 MS.gene051976:CDS
TATACTTGTTTGAATTTGAA+TGG 0.374199 2.2:-3473543 MS.gene051976:CDS
TATCAGATGGCAAGGGAATT+CGG 0.376992 2.2:+3473896 None:intergenic
TACAAATTACCTTATATTGT+TGG 0.397767 2.2:+3474417 None:intergenic
CAGAAGCAGTTTCCATTAGC+TGG 0.410914 2.2:+3473920 None:intergenic
TGTCGGGACTCGTGAAGATA+TGG 0.413042 2.2:+3473678 None:intergenic
TATATCTGCTTACAAGTGAT+TGG 0.427209 2.2:+3474574 None:intergenic
AGCACATAACAAACTCATAA+AGG 0.430383 2.2:-3473794 MS.gene051976:CDS
TGAAATGTTTCAGCAGCAAC+AGG 0.453392 2.2:-3473436 MS.gene051976:CDS
ATGTCACATACGGATCTCAT+AGG 0.460134 2.2:-3473461 MS.gene051976:CDS
TTCCTTCCAGTCTGAGAAGA+AGG 0.473570 2.2:+3473387 None:intergenic
AATTCTGACCAGCTTCTTCA+GGG 0.474643 2.2:-3473943 MS.gene051976:CDS
AGCAACAGGAGTTAGAGTTC+AGG 0.485902 2.2:-3473422 MS.gene051976:CDS
CAAATTCAAACAAGTATAGC+TGG 0.488784 2.2:+3473548 None:intergenic
GAATTTGAATGGAAATATGT+CGG 0.489962 2.2:-3473532 MS.gene051976:CDS
GGAATCCAAAATGCATGATC+AGG 0.490416 2.2:-3473511 MS.gene051976:CDS
CTTTAATGCATGTACATGTC+GGG 0.500813 2.2:+3473662 None:intergenic
TCGACTGGCTCATCACAATC+AGG 0.502357 2.2:-3475449 MS.gene051976:intron
GAAAGTAGGCCATTGTAGCC+TGG 0.505229 2.2:+3474452 None:intergenic
AACCAGTAGAAGAACAATGC+TGG 0.515161 2.2:+3474700 None:intergenic
TATGAGGCGAAGACAGTGTC+GGG 0.515554 2.2:-3473827 MS.gene051976:CDS
GCACTCCACTATGCTGATCC+AGG 0.517924 2.2:-3474470 MS.gene051976:CDS
TCAGAAGATCTTCCCCATGA+TGG 0.517971 2.2:+3475599 None:intergenic
AGCAGTACCATGCTATTATG+AGG 0.519657 2.2:-3473843 MS.gene051976:CDS
TCTTTAATGCATGTACATGT+CGG 0.521057 2.2:+3473661 None:intergenic
AGAACCGAGGAGAACAATAT+GGG 0.525995 2.2:-3474666 MS.gene051976:CDS
AACTCAGTGCTTTACTGTTG+TGG 0.539967 2.2:+3475507 None:intergenic
AGCATTGAGAAAACGGCCAC+CGG 0.542799 2.2:+3473626 None:intergenic
ACATCCCATATTGTTCTCCT+CGG 0.546379 2.2:+3474662 None:intergenic
CAAAAGTTTGAGCTTCAAAG+TGG 0.551748 2.2:-3475549 MS.gene051976:CDS
TTCCGCCATATCAGATGGCA+AGG 0.552797 2.2:+3473888 None:intergenic
TTAGCTGGCCCTGAAGAAGC+TGG 0.554264 2.2:+3473935 None:intergenic
GTAGAAGAACAATGCTGGAA+AGG 0.555226 2.2:+3474705 None:intergenic
TTAAAGAGAGCTAGAGGTGC+CGG 0.556911 2.2:-3473645 MS.gene051976:CDS
TCATCTTCAACTCAATCGAC+TGG 0.564458 2.2:-3475464 MS.gene051976:CDS
GATTGGCTGTGATCCATTAG+TGG 0.569834 2.2:+3474591 None:intergenic
ACCCTTCTTCTCAGACTGGA+AGG 0.577435 2.2:-3473389 MS.gene051976:CDS
CTCACATGCGGACGGATAGT+CGG 0.579968 2.2:+3473319 None:intergenic
TGTAGCCTGGATCAGCATAG+TGG 0.581932 2.2:+3474465 None:intergenic
ATGATGGACATCCGAAAACA+TGG 0.581989 2.2:+3475615 None:intergenic
GATGCTTCTGATGTCACATA+CGG 0.584773 2.2:-3473471 MS.gene051976:CDS
TATGCTGATCCAGGCTACAA+TGG 0.586886 2.2:-3474461 MS.gene051976:CDS
GGTTCTTCCGCCATATCAGA+TGG 0.587888 2.2:+3473883 None:intergenic
TCCCTTGCCATCTGATATGG+CGG 0.605293 2.2:-3473890 MS.gene051976:CDS
AGTTACCCTTCTTCTCAGAC+TGG 0.606122 2.2:-3473393 MS.gene051976:CDS
ATTCAAACAAGTATAGCTGG+TGG 0.610080 2.2:+3473551 None:intergenic
GGCCATATGAAGCAGAAAGT+AGG 0.615452 2.2:+3474438 None:intergenic
TACAAAGCTGACAAGAATGT+TGG 0.616527 2.2:-3473354 MS.gene051976:CDS
TTATGAGGCGAAGACAGTGT+CGG 0.619331 2.2:-3473828 MS.gene051976:CDS
ATCCATTAGTGGAGGATGGA+CGG 0.629213 2.2:+3474602 None:intergenic
TTACTGTTGTGGCATCCTTG+TGG 0.631295 2.2:+3475518 None:intergenic
ACAAGTCTCTCACATGCGGA+CGG 0.640777 2.2:+3473311 None:intergenic
GTCTTCGCCTCATAATAGCA+TGG 0.641090 2.2:+3473836 None:intergenic
TCCTTCCAGTCTGAGAAGAA+GGG 0.646710 2.2:+3473388 None:intergenic
CATGCATTAAAGAGAGCTAG+AGG 0.647089 2.2:-3473651 MS.gene051976:CDS
TTCTACAAGTCTCTCACATG+CGG 0.648923 2.2:+3473307 None:intergenic
TGTGATCCATTAGTGGAGGA+TGG 0.649182 2.2:+3474598 None:intergenic
ATGGACATCCGAAAACATGG+TGG 0.675905 2.2:+3475618 None:intergenic
TCCGCCATATCAGATGGCAA+GGG 0.679431 2.2:+3473889 None:intergenic
AAGAGAGCTAGAGGTGCCGG+TGG 0.680129 2.2:-3473642 MS.gene051976:CDS
TTCAACATTTAAGAGAACCG+AGG 0.691304 2.2:-3474679 MS.gene051976:CDS
TGGCTGTGATCCATTAGTGG+AGG 0.701564 2.2:+3474594 None:intergenic
AAAGTGGAAGCTCTACCACA+AGG 0.704797 2.2:-3475533 MS.gene051976:CDS
TGGACATCCGAAAACATGGT+GGG 0.707024 2.2:+3475619 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAGAAAAACTTAAATTATA+TGG + chr2.2:3473945-3473964 None:intergenic 10.0%
!!! TTAAGTTTTTCTTATAAAAT+TGG - chr2.2:3473951-3473970 MS.gene051976:CDS 10.0%
!!! TTTTTTATCTCTTTTAATTA+CGG - chr2.2:3474592-3474611 MS.gene051976:CDS 10.0%
!!! AATTATTTTGATTTCTTATG+AGG - chr2.2:3474928-3474947 MS.gene051976:intron 15.0%
!!! ATTTACAAGTTTTTTTTTGA+AGG + chr2.2:3474718-3474737 None:intergenic 15.0%
!!! TCTATTTCTTAAATTATTAC+TGG - chr2.2:3473766-3473785 MS.gene051976:intron 15.0%
!! AAAACTTGTAAATAGATGTT+AGG - chr2.2:3474725-3474744 MS.gene051976:intron 20.0%
!! TACAAATTACCTTATATTGT+TGG + chr2.2:3474565-3474584 None:intergenic 20.0%
!! TATACTTGTTTGAATTTGAA+TGG - chr2.2:3475436-3475455 MS.gene051976:intron 20.0%
!! TATATATATCTATGTTCTAG+TGG - chr2.2:3474424-3474443 MS.gene051976:intron 20.0%
!!! TTCTCATTTTGTTATGTTAT+TGG - chr2.2:3473604-3473623 MS.gene051976:CDS 20.0%
! AAATTGGAATTTGTCTTAGA+TGG - chr2.2:3473967-3473986 MS.gene051976:CDS 25.0%
! AGTGCAAATACTAATCAAAT+GGG + chr2.2:3474232-3474251 None:intergenic 25.0%
! CTATATATTGGTAAAAGCTA+TGG - chr2.2:3473915-3473934 MS.gene051976:CDS 25.0%
! GAATTTGAATGGAAATATGT+CGG - chr2.2:3475447-3475466 MS.gene051976:intron 25.0%
! TAATATCAATATCAACATGC+TGG + chr2.2:3474642-3474661 None:intergenic 25.0%
! TGCTGAATTACTTAATTTAG+TGG - chr2.2:3474197-3474216 MS.gene051976:intron 25.0%
!! AGACACTTTTCTCAAAATTT+TGG - chr2.2:3474470-3474489 MS.gene051976:CDS 25.0%
!! GACACTTTTCTCAAAATTTT+GGG - chr2.2:3474471-3474490 MS.gene051976:CDS 25.0%
!! TCAATTTTCAGTCATAACTA+TGG + chr2.2:3474976-3474995 None:intergenic 25.0%
!! TGCTTTGAATTCACATATAT+GGG + chr2.2:3475120-3475139 None:intergenic 25.0%
AGCACATAACAAACTCATAA+AGG - chr2.2:3475185-3475204 MS.gene051976:intron 30.0%
ATGATTTATGAAAGCCGTAA+AGG + chr2.2:3474844-3474863 None:intergenic 30.0%
CAAATTCAAACAAGTATAGC+TGG + chr2.2:3475434-3475453 None:intergenic 30.0%
CAGTGCAAATACTAATCAAA+TGG + chr2.2:3474233-3474252 None:intergenic 30.0%
CCATTTAGAGGAACAAATTA+AGG + chr2.2:3474784-3474803 None:intergenic 30.0%
GTGCAAATACTAATCAAATG+GGG + chr2.2:3474231-3474250 None:intergenic 30.0%
TACTACTGCAGTTTCTTAAT+AGG + chr2.2:3473641-3473660 None:intergenic 30.0%
TATATCTATGTTCTAGTGGA+TGG - chr2.2:3474428-3474447 MS.gene051976:CDS 30.0%
TATATCTGCTTACAAGTGAT+TGG + chr2.2:3474408-3474427 None:intergenic 30.0%
TCATCGAATCATCATCTATT+AGG - chr2.2:3474341-3474360 MS.gene051976:intron 30.0%
TCTTTAATGCATGTACATGT+CGG + chr2.2:3475321-3475340 None:intergenic 30.0%
TGCAAATACTAATCAAATGG+GGG + chr2.2:3474230-3474249 None:intergenic 30.0%
TTCATATGGCCAACAATATA+AGG - chr2.2:3474553-3474572 MS.gene051976:intron 30.0%
TTGATGGGAAAATAAATGGT+TGG - chr2.2:3474101-3474120 MS.gene051976:intron 30.0%
! ATCGTTGATGGGAAAATAAA+TGG - chr2.2:3474097-3474116 MS.gene051976:intron 30.0%
! CTGCTTTGAATTCACATATA+TGG + chr2.2:3475121-3475140 None:intergenic 30.0%
! GAGTTTTCAATTTCAAGAAC+AGG - chr2.2:3473490-3473509 MS.gene051976:CDS 30.0%
!! ACACCTTTAATTTGAAGTGT+CGG + chr2.2:3473682-3473701 None:intergenic 30.0%
!! CCTTAATTTGTTCCTCTAAA+TGG - chr2.2:3474781-3474800 MS.gene051976:intron 30.0%
!! GCTTTTTAGCATTGAGAAAA+CGG + chr2.2:3475363-3475382 None:intergenic 30.0%
!! TATAAATTGACTCGCTTATG+AGG + chr2.2:3474055-3474074 None:intergenic 30.0%
!!! TTCTCAAAATTTTGGGTTTC+AGG - chr2.2:3474478-3474497 MS.gene051976:CDS 30.0%
AACAATATTGTTAGTGCGGT+TGG + chr2.2:3475275-3475294 None:intergenic 35.0%
ACACATACACAGCAGATAAT+GGG + chr2.2:3474139-3474158 None:intergenic 35.0%
AGTTATGAAGTAACGTGTCA+AGG + chr2.2:3474014-3474033 None:intergenic 35.0%
ATTATGTAAACCTACCTCCT+TGG - chr2.2:3473882-3473901 MS.gene051976:CDS 35.0%
ATTCAAACAAGTATAGCTGG+TGG + chr2.2:3475431-3475450 None:intergenic 35.0%
CAAAAGTTTGAGCTTCAAAG+TGG - chr2.2:3473430-3473449 MS.gene051976:CDS 35.0%
CAAGATATCTCATCGTTGAT+GGG - chr2.2:3474086-3474105 MS.gene051976:intron 35.0%
CTTTAATGCATGTACATGTC+GGG + chr2.2:3475320-3475339 None:intergenic 35.0%
GATTAGTATTTGCACTGTGT+TGG - chr2.2:3474236-3474255 MS.gene051976:intron 35.0%
GCACAACAATATTGTTAGTG+CGG + chr2.2:3475279-3475298 None:intergenic 35.0%
TACAAAGCTGACAAGAATGT+TGG - chr2.2:3475625-3475644 MS.gene051976:CDS 35.0%
TACACATACACAGCAGATAA+TGG + chr2.2:3474140-3474159 None:intergenic 35.0%
TCGAATGTAACTATGTGAGT+CGG + chr2.2:3473741-3473760 None:intergenic 35.0%
TGATTGAACAGTCCATTTAG+AGG + chr2.2:3474796-3474815 None:intergenic 35.0%
TTCAACATTTAAGAGAACCG+AGG - chr2.2:3474300-3474319 MS.gene051976:intron 35.0%
! TATAGCTGGTGGAAACATTT+TGG + chr2.2:3475420-3475439 None:intergenic 35.0%
! TGCATATATTTGAGACCAGA+GGG - chr2.2:3474888-3474907 MS.gene051976:intron 35.0%
! TTCAACCTGATCATGCATTT+TGG + chr2.2:3475476-3475495 None:intergenic 35.0%
! TTGCATATATTTGAGACCAG+AGG - chr2.2:3474887-3474906 MS.gene051976:intron 35.0%
! TTTTGGACATGGATGTTTGT+TGG + chr2.2:3473415-3473434 None:intergenic 35.0%
!! CTGTTCTTCATAGCTTTTTC+TGG - chr2.2:3473819-3473838 MS.gene051976:CDS 35.0%
!! CTTCAAATTAAAGGTGTCTC+CGG - chr2.2:3473685-3473704 MS.gene051976:intron 35.0%
!! GAAACATTTTGGTCATGGTT+TGG + chr2.2:3475409-3475428 None:intergenic 35.0%
!!! CACTTTGAAGCTCAAACTTT+TGG + chr2.2:3473432-3473451 None:intergenic 35.0%
AAAACACCAGTTGACAAGCA+CGG + chr2.2:3473586-3473605 None:intergenic 40.0%
AAATTCTGACCAGCTTCTTC+AGG - chr2.2:3475035-3475054 MS.gene051976:intron 40.0%
AACCAGTAGAAGAACAATGC+TGG + chr2.2:3474282-3474301 None:intergenic 40.0%
AACTCAGTGCTTTACTGTTG+TGG + chr2.2:3473475-3473494 None:intergenic 40.0%
AATTCCCTTGCCATCTGATA+TGG - chr2.2:3475086-3475105 MS.gene051976:intron 40.0%
AATTCTGACCAGCTTCTTCA+GGG - chr2.2:3475036-3475055 MS.gene051976:intron 40.0%
ACATCCCATATTGTTCTCCT+CGG + chr2.2:3474320-3474339 None:intergenic 40.0%
AGAACCGAGGAGAACAATAT+GGG - chr2.2:3474313-3474332 MS.gene051976:intron 40.0%
AGACATCCATACACAGACAA+CGG + chr2.2:3473801-3473820 None:intergenic 40.0%
AGCAGTACCATGCTATTATG+AGG - chr2.2:3475136-3475155 MS.gene051976:intron 40.0%
ATGTCACATACGGATCTCAT+AGG - chr2.2:3475518-3475537 MS.gene051976:CDS 40.0%
CATGCATTAAAGAGAGCTAG+AGG - chr2.2:3475328-3475347 MS.gene051976:intron 40.0%
GATGCTTCTGATGTCACATA+CGG - chr2.2:3475508-3475527 MS.gene051976:CDS 40.0%
GCAAGATATCTCATCGTTGA+TGG - chr2.2:3474085-3474104 MS.gene051976:intron 40.0%
GGAATCCAAAATGCATGATC+AGG - chr2.2:3475468-3475487 MS.gene051976:CDS 40.0%
GTAGAAGAACAATGCTGGAA+AGG + chr2.2:3474277-3474296 None:intergenic 40.0%
TATATAGCCAGTGCTAACCA+AGG + chr2.2:3473902-3473921 None:intergenic 40.0%
TATCAGATGGCAAGGGAATT+CGG + chr2.2:3475086-3475105 None:intergenic 40.0%
TCAATATCAACATGCTGGAG+TGG + chr2.2:3474637-3474656 None:intergenic 40.0%
TCATCTTCAACTCAATCGAC+TGG - chr2.2:3473515-3473534 MS.gene051976:CDS 40.0%
TGAAATGTTTCAGCAGCAAC+AGG - chr2.2:3475543-3475562 MS.gene051976:CDS 40.0%
TGTCGATGTCTAGTCCTTTA+CGG - chr2.2:3474827-3474846 MS.gene051976:intron 40.0%
TTCCAGCATTGTTCTTCTAC+TGG - chr2.2:3474277-3474296 MS.gene051976:intron 40.0%
TTTGCACTGTGTTGGATACT+AGG - chr2.2:3474244-3474263 MS.gene051976:intron 40.0%
! ATGTTTTCGGATGTCCATCA+TGG - chr2.2:3473366-3473385 MS.gene051976:CDS 40.0%
! GAAGCTCAAACTTTTGGACA+TGG + chr2.2:3473426-3473445 None:intergenic 40.0%
! GCACCGACACTTCAAATTAA+AGG - chr2.2:3473676-3473695 MS.gene051976:CDS 40.0%
! GCGAATCAAGCTTTGATTCT+TGG + chr2.2:3475387-3475406 None:intergenic 40.0%
! TCAACATCCCACCATGTTTT+CGG - chr2.2:3473353-3473372 MS.gene051976:CDS 40.0%
! TGACACTTGTTGCTAGTTAC+AGG - chr2.2:3474993-3475012 MS.gene051976:intron 40.0%
! TGTTCTAGTGGATGGAAGTA+TGG - chr2.2:3474436-3474455 MS.gene051976:CDS 40.0%
! TGTTTTCGGATGTCCATCAT+GGG - chr2.2:3473367-3473386 MS.gene051976:CDS 40.0%
!! ATGATGGACATCCGAAAACA+TGG + chr2.2:3473367-3473386 None:intergenic 40.0%
!! GGTTAGCACTGGCTATATAT+TGG - chr2.2:3473903-3473922 MS.gene051976:CDS 40.0%
!! TGGTGGAAACATTTTGGTCA+TGG + chr2.2:3475414-3475433 None:intergenic 40.0%
AAAGTGGAAGCTCTACCACA+AGG - chr2.2:3473446-3473465 MS.gene051976:CDS 45.0%
AGCAACAGGAGTTAGAGTTC+AGG - chr2.2:3475557-3475576 MS.gene051976:CDS 45.0%
AGTTACCCTTCTTCTCAGAC+TGG - chr2.2:3475586-3475605 MS.gene051976:CDS 45.0%
ATACTAATCAAATGGGGGCG+TGG + chr2.2:3474225-3474244 None:intergenic 45.0%
ATCCATTAGTGGAGGATGGA+CGG + chr2.2:3474380-3474399 None:intergenic 45.0%
ATGGACATCCGAAAACATGG+TGG + chr2.2:3473364-3473383 None:intergenic 45.0%
CAACATGCTGGAGTGGAATA+CGG + chr2.2:3474630-3474649 None:intergenic 45.0%
CAATGTCCGTTGTCTGTGTA+TGG - chr2.2:3473792-3473811 MS.gene051976:CDS 45.0%
CAGAAGCAGTTTCCATTAGC+TGG + chr2.2:3475062-3475081 None:intergenic 45.0%
CATACACAGACAACGGACAT+TGG + chr2.2:3473794-3473813 None:intergenic 45.0%
GAGAACCGAGGAGAACAATA+TGG - chr2.2:3474312-3474331 MS.gene051976:intron 45.0%
GGCCATATGAAGCAGAAAGT+AGG + chr2.2:3474544-3474563 None:intergenic 45.0%
GGCCTACTTTCTGCTTCATA+TGG - chr2.2:3474539-3474558 MS.gene051976:intron 45.0%
GTCTTCGCCTCATAATAGCA+TGG + chr2.2:3475146-3475165 None:intergenic 45.0%
TATGCTGATCCAGGCTACAA+TGG - chr2.2:3474518-3474537 MS.gene051976:intron 45.0%
TCAGAAGATCTTCCCCATGA+TGG + chr2.2:3473383-3473402 None:intergenic 45.0%
TCCTTCCAGTCTGAGAAGAA+GGG + chr2.2:3475594-3475613 None:intergenic 45.0%
TGGACATCCGAAAACATGGT+GGG + chr2.2:3473363-3473382 None:intergenic 45.0%
TGTGATCCATTAGTGGAGGA+TGG + chr2.2:3474384-3474403 None:intergenic 45.0%
TTACTGTTGTGGCATCCTTG+TGG + chr2.2:3473464-3473483 None:intergenic 45.0%
TTAGGAGTAGATGTTGCAGC+TGG - chr2.2:3474743-3474762 MS.gene051976:intron 45.0%
TTATGAGGCGAAGACAGTGT+CGG - chr2.2:3475151-3475170 MS.gene051976:intron 45.0%
TTCCTTCCAGTCTGAGAAGA+AGG + chr2.2:3475595-3475614 None:intergenic 45.0%
! GATTGGCTGTGATCCATTAG+TGG + chr2.2:3474391-3474410 None:intergenic 45.0%
! GTTTTCGGATGTCCATCATG+GGG - chr2.2:3473368-3473387 MS.gene051976:CDS 45.0%
! TTAAAGAGAGCTAGAGGTGC+CGG - chr2.2:3475334-3475353 MS.gene051976:intron 45.0%
!!! ATAATTCATTAAATTTTAAA+AGG + chr2.2:3474681-3474700 None:intergenic 5.0%
ACCCTTCTTCTCAGACTGGA+AGG - chr2.2:3475590-3475609 MS.gene051976:CDS 50.0%
AGACACGTGTTAGTGTCCGA+AGG - chr2.2:3473712-3473731 MS.gene051976:intron 50.0%
AGCTGAACTAGTAGTCCCTC+TGG + chr2.2:3474906-3474925 None:intergenic 50.0%
ATAGCCAGTGCTAACCAAGG+AGG + chr2.2:3473899-3473918 None:intergenic 50.0%
CACTAACACGTGTCTCACAC+CGG + chr2.2:3473707-3473726 None:intergenic 50.0%
CTAGACATCGACAGAGAGCA+CGG + chr2.2:3474820-3474839 None:intergenic 50.0%
GAAAGTAGGCCATTGTAGCC+TGG + chr2.2:3474530-3474549 None:intergenic 50.0%
GGTTCTTCCGCCATATCAGA+TGG + chr2.2:3475099-3475118 None:intergenic 50.0%
GTGATTCCGTGCTTGTCAAC+TGG - chr2.2:3473577-3473596 MS.gene051976:CDS 50.0%
TATGAGGCGAAGACAGTGTC+GGG - chr2.2:3475152-3475171 MS.gene051976:intron 50.0%
TCCCTTGCCATCTGATATGG+CGG - chr2.2:3475089-3475108 MS.gene051976:intron 50.0%
TCCGCCATATCAGATGGCAA+GGG + chr2.2:3475093-3475112 None:intergenic 50.0%
TGGCTGTGATCCATTAGTGG+AGG + chr2.2:3474388-3474407 None:intergenic 50.0%
TGTAGCCTGGATCAGCATAG+TGG + chr2.2:3474517-3474536 None:intergenic 50.0%
TGTCGGGACTCGTGAAGATA+TGG + chr2.2:3475304-3475323 None:intergenic 50.0%
TTCCGCCATATCAGATGGCA+AGG + chr2.2:3475094-3475113 None:intergenic 50.0%
! AGCATTGAGAAAACGGCCAC+CGG + chr2.2:3475356-3475375 None:intergenic 50.0%
! CTATGTGAGTCGGTGTCCTT+CGG + chr2.2:3473731-3473750 None:intergenic 50.0%
! TCGACTGGCTCATCACAATC+AGG - chr2.2:3473530-3473549 MS.gene051976:CDS 50.0%
CACCGTCCATCCTCCACTAA+TGG - chr2.2:3474375-3474394 MS.gene051976:intron 55.0%
CCAGTGCTAACCAAGGAGGT+AGG + chr2.2:3473895-3473914 None:intergenic 55.0%
CCTACCTCCTTGGTTAGCAC+TGG - chr2.2:3473892-3473911 MS.gene051976:CDS 55.0%
GCACTCCACTATGCTGATCC+AGG - chr2.2:3474509-3474528 MS.gene051976:intron 55.0%
GCTTCTTCAGGGCCAGCTAA+TGG - chr2.2:3475047-3475066 MS.gene051976:intron 55.0%
GGAGGATGGACGGTGAGATT+TGG + chr2.2:3474370-3474389 None:intergenic 55.0%
TTAGCTGGCCCTGAAGAAGC+TGG + chr2.2:3475047-3475066 None:intergenic 55.0%
!! AAGAGAGCTAGAGGTGCCGG+TGG - chr2.2:3475337-3475356 MS.gene051976:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 3473320 3475681 3473320 ID=MS.gene051976
chr2.2 mRNA 3473320 3475681 3473320 ID=MS.gene051976.t1;Parent=MS.gene051976
chr2.2 exon 3475450 3475681 3475450 ID=MS.gene051976.t1.exon1;Parent=MS.gene051976.t1
chr2.2 CDS 3475450 3475681 3475450 ID=cds.MS.gene051976.t1;Parent=MS.gene051976.t1
chr2.2 exon 3474587 3474735 3474587 ID=MS.gene051976.t1.exon2;Parent=MS.gene051976.t1
chr2.2 CDS 3474587 3474735 3474587 ID=cds.MS.gene051976.t1;Parent=MS.gene051976.t1
chr2.2 exon 3474427 3474501 3474427 ID=MS.gene051976.t1.exon3;Parent=MS.gene051976.t1
chr2.2 CDS 3474427 3474501 3474427 ID=cds.MS.gene051976.t1;Parent=MS.gene051976.t1
chr2.2 exon 3473786 3473986 3473786 ID=MS.gene051976.t1.exon4;Parent=MS.gene051976.t1
chr2.2 CDS 3473786 3473986 3473786 ID=cds.MS.gene051976.t1;Parent=MS.gene051976.t1
chr2.2 exon 3473320 3473700 3473320 ID=MS.gene051976.t1.exon5;Parent=MS.gene051976.t1
chr2.2 CDS 3473320 3473700 3473320 ID=cds.MS.gene051976.t1;Parent=MS.gene051976.t1
Gene Sequence

>MS.gene051976

ATGAAGTGCTTATGTGAGAAAGACTCTGCTCATTCAACATCCCACCATGTTTTCGGATGTCCATCATGGGGAAGATCTTCTGAATCTGAAGTCCAACAAACATCCATGTCCAAAAGTTTGAGCTTCAAAGTGGAAGCTCTACCACAAGGATGCCACAACAGTAAAGCACTGAGTTTTCAATTTCAAGAACAGGATTCATCTTCAACTCAATCGACTGGCTCATCACAATCAGGTATTTGAATTGAAACACTATTTGTGTGATTCCGTGCTTGTCAACTGGTGTTTTCTCATTTTGTTATGTTATTGGTGTATATATCTCCTATTAAGAAACTGCAGTAGTACTTTGTATCTATGTAGCACCGACACTTCAAATTAAAGGTGTCTCCGGTGTGAGACACGTGTTAGTGTCCGAAGGACACCGACTCACATAGTTACATTCGATTACTTCTATTTCTTAAATTATTACTGGTGCCAATGTCCGTTGTCTGTGTATGGATGTCTGTTCTTCATAGCTTTTTCTGGTGATTGAAACAAAATGAAGTGATAGAAGTATATGATTATGATTATGTAAACCTACCTCCTTGGTTAGCACTGGCTATATATTGGTAAAAGCTATGGTCATCCATATAATTTAAGTTTTTCTTATAAAATTGGAATTTGTCTTAGATGGCTCAATCAAAATAACAAAATACCTTGACACGTTACTTCATAACTCATATTGAGAATTCAGCTCCTCATAAGCGAGTCAATTTATAGAATAATAAAGCAAGATATCTCATCGTTGATGGGAAAATAAATGGTTGGTATTCTAACACACCCATTATCTGCTGTGTATGTGTATGTGTATGTAATAACAACAAATTTTAATGTTTGAGATTGCTGAATTACTTAATTTAGTGGAGCCACGCCCCCATTTGATTAGTATTTGCACTGTGTTGGATACTAGGTCACATTCCTTTCCAGCATTGTTCTTCTACTGGTTCAACATTTAAGAGAACCGAGGAGAACAATATGGGATGTCTCATCGAATCATCATCTATTAGGAGTCCAAATCTCACCGTCCATCCTCCACTAATGGATCACAGCCAATCACTTGTAAGCAGATATATATATCTATGTTCTAGTGGATGGAAGTATGGAACATATATGCAGACACTTTTCTCAAAATTTTGGGTTTCAGGCGCATGTTGCACTCCACTATGCTGATCCAGGCTACAATGGCCTACTTTCTGCTTCATATGGCCAACAATATAAGGTAATTTGTATTCAAGATTTTTTATCTCTTTTAATTACGGTTTATTGTGTTACCGTATTCCACTCCAGCATGTTGATATTGATATTAATATTGTATGCAGTTTCCTTTTAAAATTTAATGAATTATGATTATGATCAAATCCTTCAAAAAAAAACTTGTAAATAGATGTTAGGAGTAGATGTTGCAGCTGGTACATCTTGTTTATACCTTAATTTGTTCCTCTAAATGGACTGTTCAATCATCCGTGCTCTCTGTCGATGTCTAGTCCTTTACGGCTTTCATAAATCATGATTACATCAAGACTTCTGTTTTTTGCATATATTTGAGACCAGAGGGACTACTAGTTCAGCTTCAATTATTTTGATTTCTTATGAGGTGTATTAGTTCTCTAACTCGAACCATAGTTATGACTGAAAATTGACACTTGTTGCTAGTTACAGGCTTATAGCATCATGTCATCAAATTCTGACCAGCTTCTTCAGGGCCAGCTAATGGAAACTGCTTCTGTCCGAATTCCCTTGCCATCTGATATGGCGGAAGAACCCATATATGTGAATTCAAAGCAGTACCATGCTATTATGAGGCGAAGACAGTGTCGGGCAAAACTTGAAGCACATAACAAACTCATAAAGGATCGTAAAGTAAGCACACATGCATTTCTGTTTTGTCTCTTCTATAGCATAAAATTTCTCTTAAACCAACCGCACTAACAATATTGTTGTGCAGCCATATCTTCACGAGTCCCGACATGTACATGCATTAAAGAGAGCTAGAGGTGCCGGTGGCCGTTTTCTCAATGCTAAAAAGCTCCAAGAATCAAAGCTTGATTCGCCAAACCATGACCAAAATGTTTCCACCAGCTATACTTGTTTGAATTTGAATGGAAATATGTCGGAATCCAAAATGCATGATCAGGTTGAAAACTACAGAGATGATGCTTCTGATGTCACATACGGATCTCATAGGAATGAAATGTTTCAGCAGCAACAGGAGTTAGAGTTCAGGTTATGCAGTTACCCTTCTTCTCAGACTGGAAGGAACATGCATGATTACAAAGCTGACAAGAATGTTGGTGCGAATCAACGCCGACTATCCGTCCGCATGTGA
Protein sequence

>MS.gene051976.t1

MKCLCEKDSAHSTSHHVFGCPSWGRSSESEVQQTSMSKSLSFKVEALPQGCHNSKALSFQFQEQDSSSTQSTGSSQSGHIPFQHCSSTGSTFKRTEENNMGCLIESSSIRSPNLTVHPPLMDHSQSLAHVALHYADPGYNGLLSASYGQQYKAYSIMSSNSDQLLQGQLMETASVRIPLPSDMAEEPIYVNSKQYHAIMRRRQCRAKLEAHNKLIKDRKPYLHESRHVHALKRARGAGGRFLNAKKLQESKLDSPNHDQNVSTSYTCLNLNGNMSESKMHDQVENYRDDASDVTYGSHRNEMFQQQQELEFRLCSYPSSQTGRNMHDYKADKNVGANQRRLSVRM