AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052069

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052069.t1 MTR_2g097850 94.270 925 42 3 1 924 1 915 0.0 1749
MS.gene052069.t1 MTR_4g064997 50.847 944 376 18 1 924 1 876 0.0 734
MS.gene052069.t1 MTR_4g019900 36.170 423 229 10 511 924 537 927 3.00e-64 234
MS.gene052069.t1 MTR_7g065880 30.947 433 212 12 515 924 470 838 1.88e-50 192
MS.gene052069.t1 MTR_7g065880 31.325 332 166 7 515 825 470 760 1.19e-39 158
MS.gene052069.t1 MTR_5g010160 28.539 445 240 17 526 924 209 621 1.40e-17 88.2
MS.gene052069.t1 MTR_8g098260 37.447 235 111 11 711 924 1082 1301 6.87e-17 86.7
MS.gene052069.t1 MTR_1g073360 34.615 130 63 4 755 873 445 563 1.19e-16 84.7
MS.gene052069.t1 MTR_1g073360 34.615 130 63 4 755 873 480 598 1.27e-16 84.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052069.t1 AT5G59020 30.319 940 436 34 43 924 2 780 1.15e-87 297
MS.gene052069.t1 AT2G29510 39.671 426 191 12 516 924 463 839 8.32e-73 257
MS.gene052069.t1 AT2G29510 27.327 505 318 12 7 501 36 501 3.80e-27 119
MS.gene052069.t1 AT5G01030 33.741 409 182 13 522 924 419 744 2.04e-46 179
MS.gene052069.t1 AT5G01030 33.741 409 182 13 522 924 419 744 2.04e-46 179
MS.gene052069.t1 AT5G01030 33.741 409 182 13 522 924 419 744 2.04e-46 179
MS.gene052069.t1 AT2G37930 35.294 340 149 14 589 924 299 571 2.22e-38 152
MS.gene052069.t1 AT2G37930 35.294 340 149 14 589 924 299 571 2.22e-38 152
MS.gene052069.t1 AT2G37930 35.294 340 149 14 589 924 271 543 3.09e-38 151
MS.gene052069.t1 AT2G37930 39.269 219 89 9 589 803 299 477 2.88e-29 123
MS.gene052069.t1 AT1G04490 41.880 117 51 4 753 868 288 388 4.10e-20 94.4
MS.gene052069.t1 AT1G04490 41.880 117 51 4 753 868 288 388 4.10e-20 94.4
MS.gene052069.t1 AT1G04490 41.880 117 51 4 753 868 288 388 4.10e-20 94.4
MS.gene052069.t1 AT1G04490 41.880 117 51 4 753 868 288 388 4.10e-20 94.4
MS.gene052069.t1 AT1G04490 41.880 117 51 4 753 868 288 388 4.10e-20 94.4
MS.gene052069.t1 AT1G04490 41.880 117 51 4 753 868 288 388 4.10e-20 94.4
MS.gene052069.t1 AT1G04490 41.880 117 51 4 753 868 288 388 4.10e-20 94.4
MS.gene052069.t1 AT1G04490 41.880 117 51 4 753 868 288 388 4.10e-20 94.4
MS.gene052069.t1 AT1G63520 30.688 189 100 6 686 866 310 475 1.21e-17 87.8
MS.gene052069.t1 AT2G33360 39.655 116 60 4 755 868 323 430 1.96e-17 86.7
MS.gene052069.t1 AT2G33360 39.655 116 60 4 755 868 478 585 2.74e-17 87.0
MS.gene052069.t1 AT1G04490 63.830 47 16 1 753 799 288 333 9.84e-14 74.3
MS.gene052069.t1 AT4G11450 36.420 162 87 5 711 868 474 623 1.70e-12 72.0

Find 221 sgRNAs with CRISPR-Local

Find 264 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTTCTGAAATAAATTTA+TGG 0.138393 2.2:+4752001 None:intergenic
GGCTTCACAAATAAAGTTTC+TGG 0.155528 2.2:-4752380 MS.gene052069:CDS
GTTTCTCAGTAGTTACTTTC+TGG 0.157657 2.2:+4752444 None:intergenic
GAAAACTTTACCAGGTTATT+TGG 0.162566 2.2:-4753461 MS.gene052069:CDS
TGTTGAGCTTCGGCTACTTC+TGG 0.183777 2.2:+4753342 None:intergenic
TCCAAGGTTGTTTGCATATC+AGG 0.215229 2.2:+4752782 None:intergenic
AATATACTCCAGGTTCAAAA+TGG 0.220776 2.2:+4750996 None:intergenic
TTTACATCCACATATTATTA+AGG 0.241348 2.2:-4753017 MS.gene052069:CDS
GAGGCAGACAAATCTTTAAA+AGG 0.251929 2.2:-4753553 MS.gene052069:CDS
CAAATAATCTTGTTTGACTA+TGG 0.257790 2.2:+4752744 None:intergenic
AAAGCAGGTCCAGCCCTTTA+AGG 0.262079 2.2:-4752073 MS.gene052069:CDS
TGATCAAATGGAAAAGAAAA+AGG 0.267133 2.2:-4752921 MS.gene052069:CDS
TGCCAACAGCAAATGTAAAA+AGG 0.275238 2.2:+4751787 None:intergenic
GGATCAAGTAGCCTCCTTAA+AGG 0.277156 2.2:+4752059 None:intergenic
AGATTTGTCTGCCTCATCTT+TGG 0.302391 2.2:+4753561 None:intergenic
TATGTTGCTTCAAACATTTC+AGG 0.302476 2.2:-4752119 MS.gene052069:CDS
CGGAAAGGTGAAGAGCTTCT+TGG 0.310125 2.2:+4752422 None:intergenic
AAACTCCCTTACGAACTTTC+TGG 0.316083 2.2:-4750905 MS.gene052069:CDS
TATGTACAGGAGGGTTGATT+TGG 0.316975 2.2:+4752716 None:intergenic
TTATGGCAGTTTGCTGCCTT+TGG 0.323720 2.2:+4752185 None:intergenic
ATATACTAGTAGGATGGTTT+CGG 0.327833 2.2:+4753062 None:intergenic
TGAATGTTGGGGTCCTTGAT+TGG 0.329534 2.2:-4753408 MS.gene052069:CDS
TGATAGAGTCCACACGGTTC+TGG 0.329830 2.2:+4751550 None:intergenic
TATACTAGTAGGATGGTTTC+GGG 0.333075 2.2:+4753063 None:intergenic
AACTAGGTTGTGACTCTTGC+TGG 0.337889 2.2:+4752633 None:intergenic
ATTCTAGAAGGGGTCTAAAT+TGG 0.346390 2.2:-4753270 MS.gene052069:CDS
GTTGAGCTTCGGCTACTTCT+GGG 0.350508 2.2:+4753343 None:intergenic
CGGAGAAAGCTTTGAATGTT+GGG 0.350856 2.2:-4753420 MS.gene052069:CDS
AACATTTCAGGCAACAAATC+AGG 0.356426 2.2:-4752107 MS.gene052069:CDS
TTTGAAACTTTCGGCATCAA+TGG 0.361650 2.2:+4752610 None:intergenic
GGATAGGCAGCGTAACTTGC+AGG 0.364076 2.2:+4750791 None:intergenic
CGCCTGATTCTTTCCCTCGA+TGG 0.367460 2.2:+4750871 None:intergenic
GGAGTATATTCAGTAGCTTT+TGG 0.369009 2.2:-4750983 MS.gene052069:CDS
CTTATTGAGCGCTGGAAAAC+TGG 0.372139 2.2:-4751315 MS.gene052069:CDS
AAAGGCAAACAACTTGTAGA+AGG 0.380262 2.2:-4753516 MS.gene052069:CDS
ATCAGTGCAATCTCACTCTA+TGG 0.382134 2.2:-4753218 MS.gene052069:CDS
TCTGCACGTTTGATTATTGT+TGG 0.392375 2.2:+4751239 None:intergenic
ACTTATGTGGTAATTCTAGA+AGG 0.392524 2.2:-4753282 MS.gene052069:CDS
CTAGGTAATATAAGAACAAT+TGG 0.399469 2.2:+4752839 None:intergenic
TGATGGCAGTTTGCTGCCTT+AGG 0.400480 2.2:+4752026 None:intergenic
ATGACATGGCGAACGTAATC+AGG 0.402175 2.2:+4751618 None:intergenic
TGACTTTCTCTAAGTTCATC+TGG 0.412561 2.2:+4752659 None:intergenic
TGATTGCGGAGGTTGGGATT+TGG 0.414627 2.2:-4751283 MS.gene052069:CDS
AACTGCATTCACCTTGTCTT+GGG 0.415203 2.2:+4751721 None:intergenic
CTCCATCGAGGGAAAGAATC+AGG 0.416905 2.2:-4750873 MS.gene052069:CDS
ATGCGTATAACTGATAGCTT+TGG 0.419288 2.2:+4751425 None:intergenic
TATATAGCTGTCAGATCCTC+TGG 0.421401 2.2:-4753175 MS.gene052069:CDS
TTGGACAAGGACCAGAGGTT+TGG 0.422199 2.2:-4751909 MS.gene052069:CDS
AAGGGGCTGACCATTCTTCA+TGG 0.430882 2.2:+4751806 None:intergenic
AAAATTTAGACCCTTCCAAC+TGG 0.431439 2.2:+4750758 None:intergenic
ACAACCTTGGATCAGAAGTT+AGG 0.433180 2.2:-4752770 MS.gene052069:CDS
GCGATACCTCCATGGAGTTA+TGG 0.438420 2.2:+4752168 None:intergenic
GGTCCTTGATTGGACCAGTC+TGG 0.439964 2.2:-4753398 MS.gene052069:CDS
TGAATGTCAAGATCTATTAC+AGG 0.440514 2.2:-4751397 MS.gene052069:CDS
GAATCAGGCGGAATCTCTAT+TGG 0.444298 2.2:-4750858 MS.gene052069:CDS
CCACTTTCCTTAATAATATG+TGG 0.445996 2.2:+4753010 None:intergenic
TCAGGTTTGAACCCATCAAC+AGG 0.452740 2.2:+4753625 None:intergenic
GATCAAGTAGCCTCCTTAAA+GGG 0.454541 2.2:+4752060 None:intergenic
GGTTCAAACAGGTGAACTAA+AGG 0.464778 2.2:-4750837 MS.gene052069:CDS
AGGATCTGACAGCTATATAA+TGG 0.464917 2.2:+4753179 None:intergenic
ATAGCAGCAAGCTCGTCATC+TGG 0.464983 2.2:+4751462 None:intergenic
ATCCACCATTGCAATGCAAA+TGG 0.465100 2.2:+4750931 None:intergenic
AACAGTGCGCTTCCTTTCCT+CGG 0.465138 2.2:+4752508 None:intergenic
CCGGAGAAAGCTTTGAATGT+TGG 0.465166 2.2:-4753421 MS.gene052069:CDS
CAACTGCATTCACCTTGTCT+TGG 0.465216 2.2:+4751720 None:intergenic
GAATCTCTATTGGTTCAAAC+AGG 0.465357 2.2:-4750848 MS.gene052069:CDS
TACTAGAAGATGTTGAGCTT+CGG 0.467189 2.2:+4753332 None:intergenic
CATTCCCATTCTGACGGGTA+CGG 0.468055 2.2:+4752553 None:intergenic
ATGAGTAGAAAACCTGTTGA+TGG 0.468639 2.2:-4753637 MS.gene052069:CDS
CCAACATTCAAAGCTTTCTC+CGG 0.470901 2.2:+4753421 None:intergenic
CCATGCTGAAGCTAAGTTGA+CGG 0.471516 2.2:+4752402 None:intergenic
TATGTGTGGCTACGGAGCTC+TGG 0.472752 2.2:+4752330 None:intergenic
CTTTCGGCATCAATGGAACT+AGG 0.473479 2.2:+4752617 None:intergenic
GATCCTGTGATTGCGGAGGT+TGG 0.473723 2.2:-4751290 MS.gene052069:CDS
ATCCTGTGATTGCGGAGGTT+GGG 0.475937 2.2:-4751289 MS.gene052069:CDS
GTGAAGCTGCCTCGTAAGTC+CGG 0.479412 2.2:+4752972 None:intergenic
AGTGATAAACCTGGAAATGC+TGG 0.482598 2.2:-4752257 MS.gene052069:CDS
ACCCTTCCAACTGGAGAGAC+AGG 0.483381 2.2:+4750767 None:intergenic
CTCCTGTCTCTCCAGTTGGA+AGG 0.483766 2.2:-4750769 MS.gene052069:CDS
GAGCACGCAAGATGATCAAA+TGG 0.485322 2.2:-4752933 MS.gene052069:CDS
GCTGCCTTTGGTTTCACCAG+TGG 0.487185 2.2:+4752197 None:intergenic
ACAAATAAGAGTGGTAGGTC+AGG 0.487236 2.2:-4751969 MS.gene052069:CDS
CTCTGCAAATGCACCTCCTA+CGG 0.488282 2.2:-4752474 MS.gene052069:CDS
TTGGTTTCACCAGTGGATCA+AGG 0.488599 2.2:+4752204 None:intergenic
TCCTGTCTCTCCAGTTGGAA+GGG 0.491356 2.2:-4750768 MS.gene052069:CDS
AAGGAATCTCCTCAATGGGC+TGG 0.492298 2.2:+4752223 None:intergenic
GATCAAGGAATCTCCTCAAT+GGG 0.496086 2.2:+4752219 None:intergenic
TATCCTCCTGTCTCTCCAGT+TGG 0.497408 2.2:-4750773 MS.gene052069:CDS
GAGGAGATTCCTTGATCCAC+TGG 0.497481 2.2:-4752213 MS.gene052069:CDS
GGATCAAGGAATCTCCTCAA+TGG 0.498648 2.2:+4752218 None:intergenic
CTCCATTTGCATTGCAATGG+TGG 0.500869 2.2:-4750933 MS.gene052069:CDS
GGCACCCGTACCCGTCAGAA+TGG 0.502194 2.2:-4752558 MS.gene052069:CDS
GGCAGCTTCACATGCTAAGT+TGG 0.502580 2.2:-4752960 MS.gene052069:CDS
TTTCCAGGTTTATCACTGCC+TGG 0.503165 2.2:+4752263 None:intergenic
CTTGATCCAGAAAGTTCGTA+AGG 0.503613 2.2:+4750899 None:intergenic
AAGAGTGGTAGGTCAGGTAA+TGG 0.504626 2.2:-4751963 MS.gene052069:CDS
GGCAACAAATCAGGTGCTGC+TGG 0.505394 2.2:-4752098 MS.gene052069:CDS
CTTCGTCGCTTATTGAGCGC+TGG 0.506867 2.2:-4751323 MS.gene052069:CDS
AAGTAGCCTCCTTAAAGGGC+TGG 0.507617 2.2:+4752064 None:intergenic
GTTCAAACAGGTGAACTAAA+GGG 0.512189 2.2:-4750836 MS.gene052069:CDS
AGAGTGGTAGGTCAGGTAAT+GGG 0.513259 2.2:-4751962 MS.gene052069:CDS
AGTGAGATTGCACTGATTGT+GGG 0.514414 2.2:+4753224 None:intergenic
CAACATTCAAAGCTTTCTCC+GGG 0.514623 2.2:+4753422 None:intergenic
GGGATTATCCTTTGACGAGA+AGG 0.515743 2.2:+4753244 None:intergenic
GAACCTTTCAATATACTAGT+AGG 0.516909 2.2:+4753052 None:intergenic
ATATACTCCAGGTTCAAAAT+GGG 0.518411 2.2:+4750997 None:intergenic
CCACATATTATTAAGGAAAG+TGG 0.519204 2.2:-4753010 MS.gene052069:CDS
GCACCCGTACCCGTCAGAAT+GGG 0.519505 2.2:-4752557 MS.gene052069:CDS
GAAATAAATTTATGGAGTGA+TGG 0.519977 2.2:+4752009 None:intergenic
CCGTCAACTTAGCTTCAGCA+TGG 0.522282 2.2:-4752402 MS.gene052069:CDS
GCATTCACCTTGTCTTGGGA+CGG 0.522795 2.2:+4751725 None:intergenic
ATCCCAACCTCCGCAATCAC+AGG 0.524007 2.2:+4751287 None:intergenic
TCTCCCCTCTCCAAATAACC+TGG 0.524321 2.2:+4753451 None:intergenic
TTTACCAGGTTATTTGGAGA+GGG 0.524450 2.2:-4753455 MS.gene052069:CDS
ATTCCCATTCTGACGGGTAC+GGG 0.525477 2.2:+4752554 None:intergenic
AGATCCTCTGGAGAGAATGC+TGG 0.527741 2.2:-4753163 MS.gene052069:CDS
AAGTTCATCTGGCAGAGGAC+AGG 0.529947 2.2:+4752670 None:intergenic
GGGAAAGATGATCAATAGTC+CGG 0.530363 2.2:+4753083 None:intergenic
TCCTGATATGCAAACAACCT+TGG 0.531394 2.2:-4752783 MS.gene052069:CDS
GAGTCCATGCAGATCTAGAA+AGG 0.532150 2.2:-4752531 MS.gene052069:CDS
CTTATGTGGTAATTCTAGAA+GGG 0.535601 2.2:-4753281 MS.gene052069:CDS
CTGAGAATATCCATGAAGAA+TGG 0.536177 2.2:-4751816 MS.gene052069:CDS
TGGTTACAAATAAGAGTGGT+AGG 0.536577 2.2:-4751974 MS.gene052069:CDS
TGAGCTTCGGCTACTTCTGG+GGG 0.537355 2.2:+4753345 None:intergenic
ATTGCGATTCAGATATGTAC+AGG 0.538827 2.2:+4752703 None:intergenic
TTCTGGTCTACTTCCGTAGG+AGG 0.541819 2.2:+4752461 None:intergenic
TGCGTATAACTGATAGCTTT+GGG 0.543220 2.2:+4751426 None:intergenic
AAAGCTACTGAATATACTCC+AGG 0.543507 2.2:+4750986 None:intergenic
GCAAATGGAGAACGCTTGCA+AGG 0.545356 2.2:+4750946 None:intergenic
CTGTGGAACAACCAAACCTC+TGG 0.545770 2.2:+4751898 None:intergenic
GTATATTGAAAGGTTCTGAC+AGG 0.547243 2.2:-4753045 MS.gene052069:CDS
TGATCCACTGCTGAAACCTA+AGG 0.547319 2.2:-4752042 MS.gene052069:CDS
AAGCACATGAAAACTTTACC+AGG 0.547766 2.2:-4753469 MS.gene052069:CDS
GTTCTCCATTTGCATTGCAA+TGG 0.547798 2.2:-4750936 MS.gene052069:CDS
ATTGGTCTCCTTCTCGTCAA+AGG 0.548579 2.2:-4753252 MS.gene052069:CDS
TTGAGCTTCGGCTACTTCTG+GGG 0.548970 2.2:+4753344 None:intergenic
GCTTCCTAACTTCTGATCCA+AGG 0.549468 2.2:+4752766 None:intergenic
CGTCAACTTAGCTTCAGCAT+GGG 0.550397 2.2:-4752401 MS.gene052069:CDS
GTTCCAGACTGGTCCAATCA+AGG 0.550773 2.2:+4753395 None:intergenic
GTTAATGCACATGATATGCT+TGG 0.550960 2.2:-4752872 MS.gene052069:CDS
CTTTCAATATACTAGTAGGA+TGG 0.558682 2.2:+4753056 None:intergenic
CAGGTGCTGCTGGTAAAAGC+AGG 0.560366 2.2:-4752088 MS.gene052069:CDS
ATTGGACCAGTCTGGAACGT+TGG 0.562574 2.2:-4753390 MS.gene052069:CDS
CATGCTAGCTCAAACATGCC+AGG 0.564429 2.2:-4752281 MS.gene052069:CDS
GCTGCCTTAGGTTTCAGCAG+TGG 0.565371 2.2:+4752038 None:intergenic
ATTTGCATTGCAATGGTGGA+TGG 0.566087 2.2:-4750929 MS.gene052069:CDS
GACGAAGGTCCACCATCACT+TGG 0.566445 2.2:+4751339 None:intergenic
CTTTACCAGGTTATTTGGAG+AGG 0.566909 2.2:-4753456 MS.gene052069:CDS
GAGTGAGATTGCACTGATTG+TGG 0.567293 2.2:+4753223 None:intergenic
GGAGAAAGCTTTGAATGTTG+GGG 0.568033 2.2:-4753419 MS.gene052069:CDS
CATCCTACTAGTATATTGAA+AGG 0.568484 2.2:-4753055 MS.gene052069:CDS
TTCTGAACTAGGTTTATGTG+TGG 0.570235 2.2:+4752316 None:intergenic
GGAAGACTATGAACTCCGCT+TGG 0.570545 2.2:+4751360 None:intergenic
TCGGCCTTTCTAGATCTGCA+TGG 0.571902 2.2:+4752527 None:intergenic
ACTAAAGCAAGGAGATACTC+AGG 0.572897 2.2:-4751499 MS.gene052069:CDS
CATAGTCTTCCAAGTGATGG+TGG 0.574925 2.2:-4751348 MS.gene052069:CDS
TTTATTTCAGAAAGAGTCTG+TGG 0.575556 2.2:-4751994 MS.gene052069:CDS
TCTCTAAGTTCATCTGGCAG+AGG 0.575764 2.2:+4752665 None:intergenic
TTAATGCACATGATATGCTT+GGG 0.581166 2.2:-4752871 MS.gene052069:CDS
AACTGGAGAGACAGGAGGAT+AGG 0.582482 2.2:+4750775 None:intergenic
ATGCAGATCTAGAAAGGCCG+AGG 0.582971 2.2:-4752525 MS.gene052069:CDS
GTACTGCCAACGTTCCAGAC+TGG 0.583526 2.2:+4753384 None:intergenic
ACTAGGTTGTGACTCTTGCT+GGG 0.586985 2.2:+4752634 None:intergenic
GGACTCATTCCCATTCTGAC+GGG 0.587176 2.2:+4752548 None:intergenic
GTGGCATCTTGTCACATGAC+CGG 0.587769 2.2:-4752991 MS.gene052069:CDS
TGAGTAGAAAACCTGTTGAT+GGG 0.591717 2.2:-4753636 MS.gene052069:CDS
ACTTTCTGGTCTACTTCCGT+AGG 0.592655 2.2:+4752458 None:intergenic
TGGACTCATTCCCATTCTGA+CGG 0.592869 2.2:+4752547 None:intergenic
GATCTAGAAAGGCCGAGGAA+AGG 0.593514 2.2:-4752520 MS.gene052069:CDS
ATCCGTACTGAGGACTTATG+TGG 0.598102 2.2:-4753295 MS.gene052069:CDS
ACAGGTGAACTAAAGGGTCA+TGG 0.599263 2.2:-4750830 MS.gene052069:CDS
TACTCAACCGTCCCAAGACA+AGG 0.600053 2.2:-4751732 MS.gene052069:CDS
GCTTCATTTGAGCAATGACA+TGG 0.600466 2.2:+4751604 None:intergenic
CTGACGGGTACGGGTGCCGA+AGG 0.603088 2.2:+4752563 None:intergenic
ATGCCAGGCAGTGATAAACC+TGG 0.604201 2.2:-4752266 MS.gene052069:CDS
GTTCATAGTCTTCCAAGTGA+TGG 0.607090 2.2:-4751351 MS.gene052069:CDS
ACTAGGTTTATGTGTGGCTA+CGG 0.607112 2.2:+4752322 None:intergenic
AAGGCAAACAACTTGTAGAA+GGG 0.607436 2.2:-4753515 MS.gene052069:CDS
AATCGTCTGAATCTAAGAGA+TGG 0.607593 2.2:-4753139 MS.gene052069:CDS
CTGAAGCTAAGTTGACGGAA+AGG 0.609361 2.2:+4752407 None:intergenic
TTGATCCAGAAAGTTCGTAA+GGG 0.612802 2.2:+4750900 None:intergenic
ATTGAGCGCTGGAAAACTGG+TGG 0.616441 2.2:-4751312 MS.gene052069:CDS
GCAGGTCCAGCCCTTTAAGG+AGG 0.616895 2.2:-4752070 MS.gene052069:CDS
CAAATGGAGAACGCTTGCAA+GGG 0.617576 2.2:+4750947 None:intergenic
AAAACAGGTCCAGCCCATTG+AGG 0.622063 2.2:-4752232 MS.gene052069:CDS
CGATTCAGATATGTACAGGA+GGG 0.624268 2.2:+4752707 None:intergenic
GTGGCTACGGAGCTCTGGTG+TGG 0.625718 2.2:+4752335 None:intergenic
TTACCAGGTTATTTGGAGAG+GGG 0.628328 2.2:-4753454 MS.gene052069:CDS
AGCAAACTGCCATAACTCCA+TGG 0.631309 2.2:-4752177 MS.gene052069:CDS
TGATCCACTGGTGAAACCAA+AGG 0.635139 2.2:-4752201 MS.gene052069:CDS
GGATCAAGAAACTCCATCGA+GGG 0.636295 2.2:-4750884 MS.gene052069:CDS
ATTTCCAGCATTCTCTCCAG+AGG 0.638927 2.2:+4753159 None:intergenic
TTATGTGGTAATTCTAGAAG+GGG 0.645972 2.2:-4753280 MS.gene052069:CDS
TCGTGAAGTTACCAAAGATG+AGG 0.646034 2.2:-4753572 MS.gene052069:CDS
AGTAGAGCTTGAGACGTCGA+AGG 0.647644 2.2:+4751837 None:intergenic
CCTAGTTCAGAAAATGTGAG+AGG 0.652170 2.2:-4752305 MS.gene052069:CDS
GCGATTCAGATATGTACAGG+AGG 0.652506 2.2:+4752706 None:intergenic
ACTGGTGGATCCTGTGATTG+CGG 0.654665 2.2:-4751297 MS.gene052069:CDS
AAGAATTGGACAAGGACCAG+AGG 0.656618 2.2:-4751914 MS.gene052069:CDS
GTCACATGACCGGACTTACG+AGG 0.659708 2.2:-4752981 MS.gene052069:CDS
CAGCGCTCAATAAGCGACGA+AGG 0.662885 2.2:+4751324 None:intergenic
TACCACATAAGTCCTCAGTA+CGG 0.668158 2.2:+4753293 None:intergenic
TTCAAATAGCCAGAACCGTG+TGG 0.669064 2.2:-4751559 MS.gene052069:CDS
CGATTCCGCAGTGTTAACTG+TGG 0.674508 2.2:+4751881 None:intergenic
TCTGTGGTTACAAATAAGAG+TGG 0.675902 2.2:-4751978 MS.gene052069:CDS
AATTTAAGAGAACAAAGTAG+TGG 0.681012 2.2:-4752896 MS.gene052069:CDS
AGATCTATTACAGGTAACAG+TGG 0.682338 2.2:-4751388 MS.gene052069:CDS
ATCAACTTCTATCCGTACTG+AGG 0.683242 2.2:-4753305 MS.gene052069:CDS
AAAGATTCAGAAAATGCGAG+AGG 0.687291 2.2:-4752143 MS.gene052069:CDS
CTTCCAACTGGAGAGACAGG+AGG 0.690282 2.2:+4750770 None:intergenic
GAGCTTCGGCTACTTCTGGG+GGG 0.691853 2.2:+4753346 None:intergenic
GGTGGATCCTGTGATTGCGG+AGG 0.700883 2.2:-4751294 MS.gene052069:CDS
GGAAGTCGGACAAATAACAC+AGG 0.702519 2.2:-4751654 MS.gene052069:CDS
CATCGAGGGAAAGAATCAGG+CGG 0.712324 2.2:-4750870 MS.gene052069:CDS
GTAACAGTGGTGCTTCCAAG+CGG 0.717660 2.2:-4751375 MS.gene052069:CDS
TGGATCAAGAAACTCCATCG+AGG 0.728422 2.2:-4750885 MS.gene052069:CDS
AAAGCAAGGAGATACTCAGG+TGG 0.728467 2.2:-4751496 MS.gene052069:CDS
GGTTGAGTACTTCACTGTGG+CGG 0.740443 2.2:+4751746 None:intergenic
GACGGTTGAGTACTTCACTG+TGG 0.749312 2.2:+4751743 None:intergenic
AAACTGCCATAACTCCATGG+AGG 0.775149 2.2:-4752174 MS.gene052069:CDS
TTGTTCCACAGTTAACACTG+CGG 0.775387 2.2:-4751886 MS.gene052069:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTGTAGATTAATCTTAGT+TGG - chr2.2:4753282-4753301 MS.gene052069:CDS 20.0%
!! TTTACATCCACATATTATTA+AGG - chr2.2:4751383-4751402 MS.gene052069:CDS 20.0%
!!! CTATACTTTTTTCACATTTA+GGG - chr2.2:4752706-4752725 MS.gene052069:CDS 20.0%
!!! CTCTTTCTGAAATAAATTTA+TGG + chr2.2:4752402-4752421 None:intergenic 20.0%
!!! TCTATACTTTTTTCACATTT+AGG - chr2.2:4752705-4752724 MS.gene052069:CDS 20.0%
! AAATGGGAAAAATTGAAAAC+AGG + chr2.2:4753390-4753409 None:intergenic 25.0%
! AATTTAAGAGAACAAAGTAG+TGG - chr2.2:4751504-4751523 MS.gene052069:CDS 25.0%
! AGATTAATCTACAATATGCT+AGG + chr2.2:4753278-4753297 None:intergenic 25.0%
! ATCAAATGGAAAAGAAAAAG+GGG - chr2.2:4751481-4751500 MS.gene052069:CDS 25.0%
! CTAGGTAATATAAGAACAAT+TGG + chr2.2:4751564-4751583 None:intergenic 25.0%
! GAAATAAATTTATGGAGTGA+TGG + chr2.2:4752394-4752413 None:intergenic 25.0%
! GATCAAATGGAAAAGAAAAA+GGG - chr2.2:4751480-4751499 MS.gene052069:CDS 25.0%
! TATACTACCTGAAAAAGAAT+TGG - chr2.2:4752472-4752491 MS.gene052069:CDS 25.0%
! TGATCAAATGGAAAAGAAAA+AGG - chr2.2:4751479-4751498 MS.gene052069:CDS 25.0%
! TTAAAAAGAAGAATGGAAGT+CGG - chr2.2:4752732-4752751 MS.gene052069:CDS 25.0%
!! CAAATAATCTTGTTTGACTA+TGG + chr2.2:4751659-4751678 None:intergenic 25.0%
!! TTTTCTGTAAAACTAAAGCA+AGG - chr2.2:4752890-4752909 MS.gene052069:CDS 25.0%
!!! AACAGGAATTTTGAAACTTT+CGG + chr2.2:4751802-4751821 None:intergenic 25.0%
!!! TACAGTGATTGTTTTTATGA+GGG + chr2.2:4751593-4751612 None:intergenic 25.0%
AAGCTATGAATGCTAAATAC+AGG - chr2.2:4753338-4753357 MS.gene052069:CDS 30.0%
AATAACACAGGAAAAAGCAA+AGG - chr2.2:4752758-4752777 MS.gene052069:CDS 30.0%
AATATACTCCAGGTTCAAAA+TGG + chr2.2:4753407-4753426 None:intergenic 30.0%
ACTTATGTGGTAATTCTAGA+AGG - chr2.2:4751118-4751137 MS.gene052069:intron 30.0%
AGCTATGAATGCTAAATACA+GGG - chr2.2:4753339-4753358 MS.gene052069:CDS 30.0%
ATATACTAGTAGGATGGTTT+CGG + chr2.2:4751341-4751360 None:intergenic 30.0%
ATATACTCCAGGTTCAAAAT+GGG + chr2.2:4753406-4753425 None:intergenic 30.0%
CATCCTACTAGTATATTGAA+AGG - chr2.2:4751345-4751364 MS.gene052069:CDS 30.0%
CCACATATTATTAAGGAAAG+TGG - chr2.2:4751390-4751409 MS.gene052069:CDS 30.0%
CCACTTTCCTTAATAATATG+TGG + chr2.2:4751393-4751412 None:intergenic 30.0%
CTTTCAATATACTAGTAGGA+TGG + chr2.2:4751347-4751366 None:intergenic 30.0%
GAAAACTTTACCAGGTTATT+TGG - chr2.2:4750939-4750958 MS.gene052069:CDS 30.0%
GAACCTTTCAATATACTAGT+AGG + chr2.2:4751351-4751370 None:intergenic 30.0%
GGATAACCAACTTAAATCAA+TGG + chr2.2:4753253-4753272 None:intergenic 30.0%
TAATCTACAATATGCTAGGA+AGG + chr2.2:4753274-4753293 None:intergenic 30.0%
TGAATGTCAAGATCTATTAC+AGG - chr2.2:4753003-4753022 MS.gene052069:CDS 30.0%
TTAATGCACATGATATGCTT+GGG - chr2.2:4751529-4751548 MS.gene052069:CDS 30.0%
TTTATTTCAGAAAGAGTCTG+TGG - chr2.2:4752406-4752425 MS.gene052069:CDS 30.0%
! AATTTTTCCCATTTTGAACC+TGG - chr2.2:4753396-4753415 MS.gene052069:CDS 30.0%
! CTTATGTGGTAATTCTAGAA+GGG - chr2.2:4751119-4751138 MS.gene052069:intron 30.0%
! TATGTTGCTTCAAACATTTC+AGG - chr2.2:4752281-4752300 MS.gene052069:CDS 30.0%
! TTATGTGGTAATTCTAGAAG+GGG - chr2.2:4751120-4751139 MS.gene052069:intron 30.0%
!! AAGGTTTTTCGAATCAACAA+AGG - chr2.2:4750866-4750885 MS.gene052069:CDS 30.0%
!! TCTCTTCCATTGATTTAAGT+TGG - chr2.2:4753244-4753263 MS.gene052069:CDS 30.0%
!!! CTACAGTGATTGTTTTTATG+AGG + chr2.2:4751594-4751613 None:intergenic 30.0%
!!! TACTTTTTTCACATTTAGGG+AGG - chr2.2:4752709-4752728 MS.gene052069:CDS 30.0%
!!! TTCTACTTTTTCAGAACCTT+CGG - chr2.2:4751821-4751840 MS.gene052069:CDS 30.0%
!!! TTCTTTTTCAGGTAGTATAG+TGG + chr2.2:4752471-4752490 None:intergenic 30.0%
AAAGATTCAGAAAATGCGAG+AGG - chr2.2:4752257-4752276 MS.gene052069:CDS 35.0%
AAAGCTACTGAATATACTCC+AGG + chr2.2:4753417-4753436 None:intergenic 35.0%
AAAGGCAAACAACTTGTAGA+AGG - chr2.2:4750884-4750903 MS.gene052069:CDS 35.0%
AACATTTCAGGCAACAAATC+AGG - chr2.2:4752293-4752312 MS.gene052069:CDS 35.0%
AAGCACATGAAAACTTTACC+AGG - chr2.2:4750931-4750950 MS.gene052069:CDS 35.0%
AAGGCAAACAACTTGTAGAA+GGG - chr2.2:4750885-4750904 MS.gene052069:CDS 35.0%
AATCGTCTGAATCTAAGAGA+TGG - chr2.2:4751261-4751280 MS.gene052069:CDS 35.0%
ACATACAGCGAAAAAATGTC+CGG - chr2.2:4751298-4751317 MS.gene052069:CDS 35.0%
ACCTGAAAAAGAATTGGACA+AGG - chr2.2:4752478-4752497 MS.gene052069:CDS 35.0%
AGATCTATTACAGGTAACAG+TGG - chr2.2:4753012-4753031 MS.gene052069:CDS 35.0%
AGGATCTGACAGCTATATAA+TGG + chr2.2:4751224-4751243 None:intergenic 35.0%
AGGGAGGTTAAAAAGAAGAA+TGG - chr2.2:4752725-4752744 MS.gene052069:CDS 35.0%
ATGAGTAGAAAACCTGTTGA+TGG - chr2.2:4750763-4750782 MS.gene052069:CDS 35.0%
ATGCGTATAACTGATAGCTT+TGG + chr2.2:4752978-4752997 None:intergenic 35.0%
ATTGCGATTCAGATATGTAC+AGG + chr2.2:4751700-4751719 None:intergenic 35.0%
CCAACAGCAAATGTAAAAAG+GGG + chr2.2:4752614-4752633 None:intergenic 35.0%
CTGAGAATATCCATGAAGAA+TGG - chr2.2:4752584-4752603 MS.gene052069:CDS 35.0%
GAGGCAGACAAATCTTTAAA+AGG - chr2.2:4750847-4750866 MS.gene052069:CDS 35.0%
GCCAACAGCAAATGTAAAAA+GGG + chr2.2:4752615-4752634 None:intergenic 35.0%
GCTATGAATGCTAAATACAG+GGG - chr2.2:4753340-4753359 MS.gene052069:CDS 35.0%
GGCTTCACAAATAAAGTTTC+TGG - chr2.2:4752020-4752039 MS.gene052069:CDS 35.0%
GGGAAAAATTGAAAACAGGA+CGG + chr2.2:4753386-4753405 None:intergenic 35.0%
GTTAATGCACATGATATGCT+TGG - chr2.2:4751528-4751547 MS.gene052069:CDS 35.0%
GTTCAAACAGGTGAACTAAA+GGG - chr2.2:4753564-4753583 MS.gene052069:CDS 35.0%
TACTAGAAGATGTTGAGCTT+CGG + chr2.2:4751071-4751090 None:intergenic 35.0%
TATACTAGTAGGATGGTTTC+GGG + chr2.2:4751340-4751359 None:intergenic 35.0%
TCAATGGAAGAGAAAATACC+TGG + chr2.2:4753237-4753256 None:intergenic 35.0%
TCTGCACGTTTGATTATTGT+TGG + chr2.2:4753164-4753183 None:intergenic 35.0%
TCTGTGGTTACAAATAAGAG+TGG - chr2.2:4752422-4752441 MS.gene052069:CDS 35.0%
TGACTTTCTCTAAGTTCATC+TGG + chr2.2:4751744-4751763 None:intergenic 35.0%
TGAGTAGAAAACCTGTTGAT+GGG - chr2.2:4750764-4750783 MS.gene052069:CDS 35.0%
TGCCAACAGCAAATGTAAAA+AGG + chr2.2:4752616-4752635 None:intergenic 35.0%
TGCGTATAACTGATAGCTTT+GGG + chr2.2:4752977-4752996 None:intergenic 35.0%
TGGTTACAAATAAGAGTGGT+AGG - chr2.2:4752426-4752445 MS.gene052069:CDS 35.0%
TTCTGAACTAGGTTTATGTG+TGG + chr2.2:4752087-4752106 None:intergenic 35.0%
TTGATCCAGAAAGTTCGTAA+GGG + chr2.2:4753503-4753522 None:intergenic 35.0%
TTTACCAGGTTATTTGGAGA+GGG - chr2.2:4750945-4750964 MS.gene052069:CDS 35.0%
! ATTCTAGAAGGGGTCTAAAT+TGG - chr2.2:4751130-4751149 MS.gene052069:intron 35.0%
! GGAGTATATTCAGTAGCTTT+TGG - chr2.2:4753417-4753436 MS.gene052069:CDS 35.0%
! GTATATTGAAAGGTTCTGAC+AGG - chr2.2:4751355-4751374 MS.gene052069:CDS 35.0%
! GTTTCTCAGTAGTTACTTTC+TGG + chr2.2:4751959-4751978 None:intergenic 35.0%
! TACGACAAAGAAGAGTTTTC+AGG + chr2.2:4750796-4750815 None:intergenic 35.0%
! TCCTTGTCCAATTCTTTTTC+AGG + chr2.2:4752482-4752501 None:intergenic 35.0%
! TTTGAAACTTTCGGCATCAA+TGG + chr2.2:4751793-4751812 None:intergenic 35.0%
! TTTTTATGAGGGTTGTCTCT+AGG + chr2.2:4751582-4751601 None:intergenic 35.0%
!! GAATCTCTATTGGTTCAAAC+AGG - chr2.2:4753552-4753571 MS.gene052069:CDS 35.0%
!! GGTTCTGAAAAAGTAGAAAC+AGG + chr2.2:4751819-4751838 None:intergenic 35.0%
AAACTCCCTTACGAACTTTC+TGG - chr2.2:4753495-4753514 MS.gene052069:CDS 40.0%
AACTGCATTCACCTTGTCTT+GGG + chr2.2:4752682-4752701 None:intergenic 40.0%
ACAAATAAGAGTGGTAGGTC+AGG - chr2.2:4752431-4752450 MS.gene052069:CDS 40.0%
ACAACCTTGGATCAGAAGTT+AGG - chr2.2:4751630-4751649 MS.gene052069:CDS 40.0%
ACTAAAGCAAGGAGATACTC+AGG - chr2.2:4752901-4752920 MS.gene052069:CDS 40.0%
ACTAGGTTTATGTGTGGCTA+CGG + chr2.2:4752081-4752100 None:intergenic 40.0%
AGATTTGTCTGCCTCATCTT+TGG + chr2.2:4750842-4750861 None:intergenic 40.0%
AGTGATAAACCTGGAAATGC+TGG - chr2.2:4752143-4752162 MS.gene052069:CDS 40.0%
ATCAACTTCTATCCGTACTG+AGG - chr2.2:4751095-4751114 MS.gene052069:intron 40.0%
ATCAGTGCAATCTCACTCTA+TGG - chr2.2:4751182-4751201 MS.gene052069:intron 40.0%
ATCCACCATTGCAATGCAAA+TGG + chr2.2:4753472-4753491 None:intergenic 40.0%
CAACATTCAAAGCTTTCTCC+GGG + chr2.2:4750981-4751000 None:intergenic 40.0%
CCAACATTCAAAGCTTTCTC+CGG + chr2.2:4750982-4751001 None:intergenic 40.0%
CCTAGTTCAGAAAATGTGAG+AGG - chr2.2:4752095-4752114 MS.gene052069:CDS 40.0%
CGATTCAGATATGTACAGGA+GGG + chr2.2:4751696-4751715 None:intergenic 40.0%
CTTGATCCAGAAAGTTCGTA+AGG + chr2.2:4753504-4753523 None:intergenic 40.0%
CTTTACCAGGTTATTTGGAG+AGG - chr2.2:4750944-4750963 MS.gene052069:CDS 40.0%
GATCAAGGAATCTCCTCAAT+GGG + chr2.2:4752184-4752203 None:intergenic 40.0%
GATCAAGTAGCCTCCTTAAA+GGG + chr2.2:4752343-4752362 None:intergenic 40.0%
GCTTCATTTGAGCAATGACA+TGG + chr2.2:4752799-4752818 None:intergenic 40.0%
GGGAAAGATGATCAATAGTC+CGG + chr2.2:4751320-4751339 None:intergenic 40.0%
GGTTCAAACAGGTGAACTAA+AGG - chr2.2:4753563-4753582 MS.gene052069:CDS 40.0%
GTTCATAGTCTTCCAAGTGA+TGG - chr2.2:4753049-4753068 MS.gene052069:CDS 40.0%
GTTCTCCATTTGCATTGCAA+TGG - chr2.2:4753464-4753483 MS.gene052069:CDS 40.0%
TACCACATAAGTCCTCAGTA+CGG + chr2.2:4751110-4751129 None:intergenic 40.0%
TATATAGCTGTCAGATCCTC+TGG - chr2.2:4751225-4751244 MS.gene052069:CDS 40.0%
TCCAAGGTTGTTTGCATATC+AGG + chr2.2:4751621-4751640 None:intergenic 40.0%
TCCTGATATGCAAACAACCT+TGG - chr2.2:4751617-4751636 MS.gene052069:CDS 40.0%
TCGTGAAGTTACCAAAGATG+AGG - chr2.2:4750828-4750847 MS.gene052069:CDS 40.0%
TTACCAGGTTATTTGGAGAG+GGG - chr2.2:4750946-4750965 MS.gene052069:CDS 40.0%
TTGTTCCACAGTTAACACTG+CGG - chr2.2:4752514-4752533 MS.gene052069:CDS 40.0%
! ATCTTTTTGCGATACCTCCA+TGG + chr2.2:4752243-4752262 None:intergenic 40.0%
! ATTTGCATTGCAATGGTGGA+TGG - chr2.2:4753471-4753490 MS.gene052069:CDS 40.0%
! CCTCTCACATTTTCTGAACT+AGG + chr2.2:4752098-4752117 None:intergenic 40.0%
! CTTTTGTGATAGAGTCCACA+CGG + chr2.2:4752859-4752878 None:intergenic 40.0%
! TATGTACAGGAGGGTTGATT+TGG + chr2.2:4751687-4751706 None:intergenic 40.0%
! TCAGTATGAGCTTTTCTTCC+AGG - chr2.2:4753216-4753235 MS.gene052069:CDS 40.0%
!! AGTGAGATTGCACTGATTGT+GGG + chr2.2:4751179-4751198 None:intergenic 40.0%
!! CGGAGAAAGCTTTGAATGTT+GGG - chr2.2:4750980-4750999 MS.gene052069:CDS 40.0%
!! GGAGAAAGCTTTGAATGTTG+GGG - chr2.2:4750981-4751000 MS.gene052069:CDS 40.0%
!!! CCCCTTTTTACATTTGCTGT+TGG - chr2.2:4752611-4752630 MS.gene052069:CDS 40.0%
AAACTGCCATAACTCCATGG+AGG - chr2.2:4752226-4752245 MS.gene052069:CDS 45.0%
AAAGCAAGGAGATACTCAGG+TGG - chr2.2:4752904-4752923 MS.gene052069:CDS 45.0%
AACTAGGTTGTGACTCTTGC+TGG + chr2.2:4751770-4751789 None:intergenic 45.0%
AAGAATTGGACAAGGACCAG+AGG - chr2.2:4752486-4752505 MS.gene052069:CDS 45.0%
AAGAGTGGTAGGTCAGGTAA+TGG - chr2.2:4752437-4752456 MS.gene052069:CDS 45.0%
ACAGGTGAACTAAAGGGTCA+TGG - chr2.2:4753570-4753589 MS.gene052069:CDS 45.0%
ACTAGGTTGTGACTCTTGCT+GGG + chr2.2:4751769-4751788 None:intergenic 45.0%
AGAGTGGTAGGTCAGGTAAT+GGG - chr2.2:4752438-4752457 MS.gene052069:CDS 45.0%
AGCAAACTGCCATAACTCCA+TGG - chr2.2:4752223-4752242 MS.gene052069:CDS 45.0%
ATCCGTACTGAGGACTTATG+TGG - chr2.2:4751105-4751124 MS.gene052069:intron 45.0%
ATGACATGGCGAACGTAATC+AGG + chr2.2:4752785-4752804 None:intergenic 45.0%
ATTTCCAGCATTCTCTCCAG+AGG + chr2.2:4751244-4751263 None:intergenic 45.0%
CAAATGGAGAACGCTTGCAA+GGG + chr2.2:4753456-4753475 None:intergenic 45.0%
CAACTGCATTCACCTTGTCT+TGG + chr2.2:4752683-4752702 None:intergenic 45.0%
CATAGTCTTCCAAGTGATGG+TGG - chr2.2:4753052-4753071 MS.gene052069:CDS 45.0%
CGTCAACTTAGCTTCAGCAT+GGG - chr2.2:4751999-4752018 MS.gene052069:CDS 45.0%
CTCCATTTGCATTGCAATGG+TGG - chr2.2:4753467-4753486 MS.gene052069:CDS 45.0%
CTGGAAATGCTGGCAAAAAC+AGG - chr2.2:4752153-4752172 MS.gene052069:CDS 45.0%
CTTTCGGCATCAATGGAACT+AGG + chr2.2:4751786-4751805 None:intergenic 45.0%
GAATCAGGCGGAATCTCTAT+TGG - chr2.2:4753542-4753561 MS.gene052069:CDS 45.0%
GAGCACGCAAGATGATCAAA+TGG - chr2.2:4751467-4751486 MS.gene052069:CDS 45.0%
GAGTCCATGCAGATCTAGAA+AGG - chr2.2:4751869-4751888 MS.gene052069:CDS 45.0%
GCGATTCAGATATGTACAGG+AGG + chr2.2:4751697-4751716 None:intergenic 45.0%
GCTTCCTAACTTCTGATCCA+AGG + chr2.2:4751637-4751656 None:intergenic 45.0%
GGAAGTCGGACAAATAACAC+AGG - chr2.2:4752746-4752765 MS.gene052069:CDS 45.0%
GGATCAAGAAACTCCATCGA+GGG - chr2.2:4753516-4753535 MS.gene052069:CDS 45.0%
GGATCAAGGAATCTCCTCAA+TGG + chr2.2:4752185-4752204 None:intergenic 45.0%
GGATCAAGTAGCCTCCTTAA+AGG + chr2.2:4752344-4752363 None:intergenic 45.0%
GGGATTATCCTTTGACGAGA+AGG + chr2.2:4751159-4751178 None:intergenic 45.0%
TCAGGTTTGAACCCATCAAC+AGG + chr2.2:4750778-4750797 None:intergenic 45.0%
TCTCTAAGTTCATCTGGCAG+AGG + chr2.2:4751738-4751757 None:intergenic 45.0%
TGAATGTTGGGGTCCTTGAT+TGG - chr2.2:4750992-4751011 MS.gene052069:CDS 45.0%
TGATCCACTGCTGAAACCTA+AGG - chr2.2:4752358-4752377 MS.gene052069:CDS 45.0%
TGATCCACTGGTGAAACCAA+AGG - chr2.2:4752199-4752218 MS.gene052069:CDS 45.0%
TGGACTCATTCCCATTCTGA+CGG + chr2.2:4751856-4751875 None:intergenic 45.0%
TGGATCAAGAAACTCCATCG+AGG - chr2.2:4753515-4753534 MS.gene052069:CDS 45.0%
TTCAAATAGCCAGAACCGTG+TGG - chr2.2:4752841-4752860 MS.gene052069:CDS 45.0%
TTGGTTTCACCAGTGGATCA+AGG + chr2.2:4752199-4752218 None:intergenic 45.0%
TTTCCAGGTTTATCACTGCC+TGG + chr2.2:4752140-4752159 None:intergenic 45.0%
! ACTTTCTGGTCTACTTCCGT+AGG + chr2.2:4751945-4751964 None:intergenic 45.0%
! ATTGGTCTCCTTCTCGTCAA+AGG - chr2.2:4751148-4751167 MS.gene052069:intron 45.0%
! CCATGCTGAAGCTAAGTTGA+CGG + chr2.2:4752001-4752020 None:intergenic 45.0%
! CTGAAGCTAAGTTGACGGAA+AGG + chr2.2:4751996-4752015 None:intergenic 45.0%
!! CCGGAGAAAGCTTTGAATGT+TGG - chr2.2:4750979-4750998 MS.gene052069:CDS 45.0%
!! CTTATTGAGCGCTGGAAAAC+TGG - chr2.2:4753085-4753104 MS.gene052069:CDS 45.0%
!! GAGTGAGATTGCACTGATTG+TGG + chr2.2:4751180-4751199 None:intergenic 45.0%
!! TTATGGCAGTTTGCTGCCTT+TGG + chr2.2:4752218-4752237 None:intergenic 45.0%
!!! CTGTTTTTGCCAGCATTTCC+AGG + chr2.2:4752155-4752174 None:intergenic 45.0%
AAAACAGGTCCAGCCCATTG+AGG - chr2.2:4752168-4752187 MS.gene052069:CDS 50.0%
AAAGCAGGTCCAGCCCTTTA+AGG - chr2.2:4752327-4752346 MS.gene052069:CDS 50.0%
AACAGTGCGCTTCCTTTCCT+CGG + chr2.2:4751895-4751914 None:intergenic 50.0%
AACTGGAGAGACAGGAGGAT+AGG + chr2.2:4753628-4753647 None:intergenic 50.0%
AAGGAATCTCCTCAATGGGC+TGG + chr2.2:4752180-4752199 None:intergenic 50.0%
AAGGGGCTGACCATTCTTCA+TGG + chr2.2:4752597-4752616 None:intergenic 50.0%
AAGTAGCCTCCTTAAAGGGC+TGG + chr2.2:4752339-4752358 None:intergenic 50.0%
AAGTTCATCTGGCAGAGGAC+AGG + chr2.2:4751733-4751752 None:intergenic 50.0%
AGTAGAGCTTGAGACGTCGA+AGG + chr2.2:4752566-4752585 None:intergenic 50.0%
ATAGCAGCAAGCTCGTCATC+TGG + chr2.2:4752941-4752960 None:intergenic 50.0%
ATCCTGTGATTGCGGAGGTT+GGG - chr2.2:4753111-4753130 MS.gene052069:CDS 50.0%
ATGCAGATCTAGAAAGGCCG+AGG - chr2.2:4751875-4751894 MS.gene052069:CDS 50.0%
ATGCCAGGCAGTGATAAACC+TGG - chr2.2:4752134-4752153 MS.gene052069:CDS 50.0%
CATCGAGGGAAAGAATCAGG+CGG - chr2.2:4753530-4753549 MS.gene052069:CDS 50.0%
CATGCTAGCTCAAACATGCC+AGG - chr2.2:4752119-4752138 MS.gene052069:CDS 50.0%
CCGTCAACTTAGCTTCAGCA+TGG - chr2.2:4751998-4752017 MS.gene052069:CDS 50.0%
CGATTCCGCAGTGTTAACTG+TGG + chr2.2:4752522-4752541 None:intergenic 50.0%
CGGAAAGGTGAAGAGCTTCT+TGG + chr2.2:4751981-4752000 None:intergenic 50.0%
CTCCATCGAGGGAAAGAATC+AGG - chr2.2:4753527-4753546 MS.gene052069:CDS 50.0%
CTCTGCAAATGCACCTCCTA+CGG - chr2.2:4751926-4751945 MS.gene052069:CDS 50.0%
CTGTGGAACAACCAAACCTC+TGG + chr2.2:4752505-4752524 None:intergenic 50.0%
GACGGTTGAGTACTTCACTG+TGG + chr2.2:4752660-4752679 None:intergenic 50.0%
GAGGAGATTCCTTGATCCAC+TGG - chr2.2:4752187-4752206 MS.gene052069:CDS 50.0%
GATCTAGAAAGGCCGAGGAA+AGG - chr2.2:4751880-4751899 MS.gene052069:CDS 50.0%
GCAAATGGAGAACGCTTGCA+AGG + chr2.2:4753457-4753476 None:intergenic 50.0%
GCATTCACCTTGTCTTGGGA+CGG + chr2.2:4752678-4752697 None:intergenic 50.0%
GCGATACCTCCATGGAGTTA+TGG + chr2.2:4752235-4752254 None:intergenic 50.0%
GGAAGACTATGAACTCCGCT+TGG + chr2.2:4753043-4753062 None:intergenic 50.0%
GGACTCATTCCCATTCTGAC+GGG + chr2.2:4751855-4751874 None:intergenic 50.0%
GGAGAGGGGAGAAAAAATCC+CGG - chr2.2:4750960-4750979 MS.gene052069:CDS 50.0%
GGCAGCTTCACATGCTAAGT+TGG - chr2.2:4751440-4751459 MS.gene052069:CDS 50.0%
GGTTGAGTACTTCACTGTGG+CGG + chr2.2:4752657-4752676 None:intergenic 50.0%
GTAACAGTGGTGCTTCCAAG+CGG - chr2.2:4753025-4753044 MS.gene052069:CDS 50.0%
GTGGCATCTTGTCACATGAC+CGG - chr2.2:4751409-4751428 MS.gene052069:CDS 50.0%
GTTGAGCTTCGGCTACTTCT+GGG + chr2.2:4751060-4751079 None:intergenic 50.0%
TACTCAACCGTCCCAAGACA+AGG - chr2.2:4752668-4752687 MS.gene052069:CDS 50.0%
TATCCTCCTGTCTCTCCAGT+TGG - chr2.2:4753627-4753646 MS.gene052069:CDS 50.0%
TCCTGTCTCTCCAGTTGGAA+GGG - chr2.2:4753632-4753651 MS.gene052069:CDS 50.0%
TCGGCCTTTCTAGATCTGCA+TGG + chr2.2:4751876-4751895 None:intergenic 50.0%
TCTCCCCTCTCCAAATAACC+TGG + chr2.2:4750952-4750971 None:intergenic 50.0%
TGATAGAGTCCACACGGTTC+TGG + chr2.2:4752853-4752872 None:intergenic 50.0%
TGATTGCGGAGGTTGGGATT+TGG - chr2.2:4753117-4753136 MS.gene052069:CDS 50.0%
TGTTGAGCTTCGGCTACTTC+TGG + chr2.2:4751061-4751080 None:intergenic 50.0%
TTCTGGTCTACTTCCGTAGG+AGG + chr2.2:4751942-4751961 None:intergenic 50.0%
TTGAGCTTCGGCTACTTCTG+GGG + chr2.2:4751059-4751078 None:intergenic 50.0%
TTGGACAAGGACCAGAGGTT+TGG - chr2.2:4752491-4752510 MS.gene052069:CDS 50.0%
! ACTGGTGGATCCTGTGATTG+CGG - chr2.2:4753103-4753122 MS.gene052069:CDS 50.0%
! AGATCCTCTGGAGAGAATGC+TGG - chr2.2:4751237-4751256 MS.gene052069:CDS 50.0%
! ATTCCCATTCTGACGGGTAC+GGG + chr2.2:4751849-4751868 None:intergenic 50.0%
! ATTGGACCAGTCTGGAACGT+TGG - chr2.2:4751010-4751029 MS.gene052069:CDS 50.0%
! CATTCCCATTCTGACGGGTA+CGG + chr2.2:4751850-4751869 None:intergenic 50.0%
! GTTCCAGACTGGTCCAATCA+AGG + chr2.2:4751008-4751027 None:intergenic 50.0%
!! ATTGAGCGCTGGAAAACTGG+TGG - chr2.2:4753088-4753107 MS.gene052069:CDS 50.0%
!! TGATGGCAGTTTGCTGCCTT+AGG + chr2.2:4752377-4752396 None:intergenic 50.0%
ACCCTTCCAACTGGAGAGAC+AGG + chr2.2:4753636-4753655 None:intergenic 55.0%
ATCCCAACCTCCGCAATCAC+AGG + chr2.2:4753116-4753135 None:intergenic 55.0%
CAGCGCTCAATAAGCGACGA+AGG + chr2.2:4753079-4753098 None:intergenic 55.0%
CGCCTGATTCTTTCCCTCGA+TGG + chr2.2:4753532-4753551 None:intergenic 55.0%
CTCCTGTCTCTCCAGTTGGA+AGG - chr2.2:4753631-4753650 MS.gene052069:CDS 55.0%
CTTCCAACTGGAGAGACAGG+AGG + chr2.2:4753633-4753652 None:intergenic 55.0%
CTTCGTCGCTTATTGAGCGC+TGG - chr2.2:4753077-4753096 MS.gene052069:CDS 55.0%
GACGAAGGTCCACCATCACT+TGG + chr2.2:4753064-4753083 None:intergenic 55.0%
GATCCTGTGATTGCGGAGGT+TGG - chr2.2:4753110-4753129 MS.gene052069:CDS 55.0%
GCTGCCTTAGGTTTCAGCAG+TGG + chr2.2:4752365-4752384 None:intergenic 55.0%
GGTCCTTGATTGGACCAGTC+TGG - chr2.2:4751002-4751021 MS.gene052069:CDS 55.0%
GTACTGCCAACGTTCCAGAC+TGG + chr2.2:4751019-4751038 None:intergenic 55.0%
GTCACATGACCGGACTTACG+AGG - chr2.2:4751419-4751438 MS.gene052069:CDS 55.0%
GTGAAGCTGCCTCGTAAGTC+CGG + chr2.2:4751431-4751450 None:intergenic 55.0%
TATGTGTGGCTACGGAGCTC+TGG + chr2.2:4752073-4752092 None:intergenic 55.0%
TGAGCTTCGGCTACTTCTGG+GGG + chr2.2:4751058-4751077 None:intergenic 55.0%
! GCTGCCTTTGGTTTCACCAG+TGG + chr2.2:4752206-4752225 None:intergenic 55.0%
! GGATAGGCAGCGTAACTTGC+AGG + chr2.2:4753612-4753631 None:intergenic 55.0%
! GGCAACAAATCAGGTGCTGC+TGG - chr2.2:4752302-4752321 MS.gene052069:CDS 55.0%
!! CAGGTGCTGCTGGTAAAAGC+AGG - chr2.2:4752312-4752331 MS.gene052069:CDS 55.0%
GAGCTTCGGCTACTTCTGGG+GGG + chr2.2:4751057-4751076 None:intergenic 60.0%
GCACCCGTACCCGTCAGAAT+GGG - chr2.2:4751843-4751862 MS.gene052069:CDS 60.0%
GGTGGATCCTGTGATTGCGG+AGG - chr2.2:4753106-4753125 MS.gene052069:CDS 60.0%
! GCAGGTCCAGCCCTTTAAGG+AGG - chr2.2:4752330-4752349 MS.gene052069:CDS 60.0%
GGCACCCGTACCCGTCAGAA+TGG - chr2.2:4751842-4751861 MS.gene052069:CDS 65.0%
! GTGGCTACGGAGCTCTGGTG+TGG + chr2.2:4752068-4752087 None:intergenic 65.0%
!! CTGACGGGTACGGGTGCCGA+AGG + chr2.2:4751840-4751859 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 4750763 4753659 4750763 ID=MS.gene052069
chr2.2 mRNA 4750763 4753659 4750763 ID=MS.gene052069.t1;Parent=MS.gene052069
chr2.2 exon 4751185 4753659 4751185 ID=MS.gene052069.t1.exon1;Parent=MS.gene052069.t1
chr2.2 CDS 4751185 4753659 4751185 ID=cds.MS.gene052069.t1;Parent=MS.gene052069.t1
chr2.2 exon 4750763 4751062 4750763 ID=MS.gene052069.t1.exon2;Parent=MS.gene052069.t1
chr2.2 CDS 4750763 4751062 4750763 ID=cds.MS.gene052069.t1;Parent=MS.gene052069.t1
Gene Sequence

>MS.gene052069

ATGAGTAGAAAACCTGTTGATGGGTTCAAACCTGAAAACTCTTCTTTGTCGTACGCTGATTTTCATCGTGAAGTTACCAAAGATGAGGCAGACAAATCTTTAAAAGGTTTTTCGAATCAACAAAGGCAAACAACTTGTAGAAGGGAAAATGATGAAGATGAGCTTGTTAAGCACATGAAAACTTTACCAGGTTATTTGGAGAGGGGAGAAAAAATCCCGGAGAAAGCTTTGAATGTTGGGGTCCTTGATTGGACCAGTCTGGAACGTTGGCAGTACAGCAATAAACTTGTACCCCCCAGAAGTAGCCGAAGCTCAACATCTTCTAGTAATACATCAACTTCTATCCGTACTGAGGACTTATGTGGTAATTCTAGAAGGGGTCTAAATTGGTCTCCTTCTCGTCAAAGGATAATCCCACAATCAGTGCAATCTCACTCTATGGCTTCTACAAAGCAAGACCATTATATAGCTGTCAGATCCTCTGGAGAGAATGCTGGAAATCGTCTGAATCTAAGAGATGGCAACAGTAGTATTGACATACAGCGAAAAAATGTCCGGACTATTGATCATCTTTCCCGAAACCATCCTACTAGTATATTGAAAGGTTCTGACAGGAAACATTTACATCCACATATTATTAAGGAAAGTGGCATCTTGTCACATGACCGGACTTACGAGGCAGCTTCACATGCTAAGTTGGAAATGAGCACGCAAGATGATCAAATGGAAAAGAAAAAGGGGAATTTAAGAGAACAAAGTAGTGGTGTTAATGCACATGATATGCTTGGGAAAAGCAAACCAATTGTTCTTATATTACCTAGAGACAACCCTCATAAAAACAATCACTGTAGAGCTCCTGATATGCAAACAACCTTGGATCAGAAGTTAGGAAGCCATAGTCAAACAAGATTATTTGTAAATCCAAATCAACCCTCCTGTACATATCTGAATCGCAATGTTTCACACTCCTGTCCTCTGCCAGATGAACTTAGAGAAAGTCATTCCCAGCAAGAGTCACAACCTAGTTCCATTGATGCCGAAAGTTTCAAAATTCCTGTTTCTACTTTTTCAGAACCTTCGGCACCCGTACCCGTCAGAATGGGAATGAGTCCATGCAGATCTAGAAAGGCCGAGGAAAGGAAGCGCACTGTTGCTGTATCTTGCTCTGCAAATGCACCTCCTACGGAAGTAGACCAGAAAGTAACTACTGAGAAACCAAGAAGCTCTTCACCTTTCCGTCAACTTAGCTTCAGCATGGGCTTCACAAATAAAGTTTCTGGTTGTAAAGAGATTGCATATGTTCCACACCAGAGCTCCGTAGCCACACATAAACCTAGTTCAGAAAATGTGAGAGGTCATGCTAGCTCAAACATGCCAGGCAGTGATAAACCTGGAAATGCTGGCAAAAACAGGTCCAGCCCATTGAGGAGATTCCTTGATCCACTGGTGAAACCAAAGGCAGCAAACTGCCATAACTCCATGGAGGTATCGCAAAAAGATTCAGAAAATGCGAGAGGTTATGTTGCTTCAAACATTTCAGGCAACAAATCAGGTGCTGCTGGTAAAAGCAGGTCCAGCCCTTTAAGGAGGCTACTTGATCCACTGCTGAAACCTAAGGCAGCAAACTGCCATCACTCCATAAATTTATTTCAGAAAGAGTCTGTGGTTACAAATAAGAGTGGTAGGTCAGGTAATGGGAAATGTTCCACTATACTACCTGAAAAAGAATTGGACAAGGACCAGAGGTTTGGTTGTTCCACAGTTAACACTGCGGAATCGTCAAGTGAAGAGAAGTATATACCTTCGACGTCTCAAGCTCTACTGAGAATATCCATGAAGAATGGTCAGCCCCTTTTTACATTTGCTGTTGGCAACAATAGCAATATTCTCGCCGCCACAGTGAAGTACTCAACCGTCCCAAGACAAGGTGAATGCAGTTGTATCTATACTTTTTTCACATTTAGGGAGGTTAAAAAGAAGAATGGAAGTCGGACAAATAACACAGGAAAAAGCAAAGGTCCTGATTACGTTCGCCATGTCATTGCTCAAATGAAGCTTTCTGATTTGCACTATGATGATTCAAATAGCCAGAACCGTGTGGACTCTATCACAAAAGAGTTTGTTTTATTTTCTGTAAAACTAAAGCAAGGAGATACTCAGGTGGCTGACTATCAGCCAGATGACGAGCTTGCTGCTATAGTTGTCAAATCACCCAAAGCTATCAGTTATACGCATCAGCATGAATGTCAAGATCTATTACAGGTAACAGTGGTGCTTCCAAGCGGAGTTCATAGTCTTCCAAGTGATGGTGGACCTTCGTCGCTTATTGAGCGCTGGAAAACTGGTGGATCCTGTGATTGCGGAGGTTGGGATTTGGCTTGTAAGCTAAAAATTCTTGCCAACAATAATCAAACGTGCAGAAAATCAAGAACATCAGAAGCTTATTTTGCAGATCAGTATGAGCTTTTCTTCCAGGTATTTTCTCTTCCATTGATTTAAGTTGGTTATCCTTCCTAGCATATTGTAGATTAATCTTAGTTGGTTAGTGTGTAGATAATTGATTATTAATTTATTTAAGCTATGAATGCTAAATACAGGGGACTGAACAATGTCAAGATAACCGTCCTGTTTTCAATTTTTCCCATTTTGAACCTGGAGTATATTCAGTAGCTTTTGGTTCATCACTTTCACCCTTGCAAGCGTTCTCCATTTGCATTGCAATGGTGGATGGCAAACTCCCTTACGAACTTTCTGGATCAAGAAACTCCATCGAGGGAAAGAATCAGGCGGAATCTCTATTGGTTCAAACAGGTGAACTAAAGGGTCATGGTAAATCTGATGACATTCCTGCAAGTTACGCTGCCTATCCTCCTGTCTCTCCAGTTGGAAGGGTCTAA
Protein sequence

>MS.gene052069.t1

MSRKPVDGFKPENSSLSYADFHREVTKDEADKSLKGFSNQQRQTTCRRENDEDELVKHMKTLPGYLERGEKIPEKALNVGVLDWTSLERWQYSNKLVPPRSSRSSTSSSNTSTSIRTEDLCGNSRRGLNWSPSRQRIIPQSVQSHSMASTKQDHYIAVRSSGENAGNRLNLRDGNSSIDIQRKNVRTIDHLSRNHPTSILKGSDRKHLHPHIIKESGILSHDRTYEAASHAKLEMSTQDDQMEKKKGNLREQSSGVNAHDMLGKSKPIVLILPRDNPHKNNHCRAPDMQTTLDQKLGSHSQTRLFVNPNQPSCTYLNRNVSHSCPLPDELRESHSQQESQPSSIDAESFKIPVSTFSEPSAPVPVRMGMSPCRSRKAEERKRTVAVSCSANAPPTEVDQKVTTEKPRSSSPFRQLSFSMGFTNKVSGCKEIAYVPHQSSVATHKPSSENVRGHASSNMPGSDKPGNAGKNRSSPLRRFLDPLVKPKAANCHNSMEVSQKDSENARGYVASNISGNKSGAAGKSRSSPLRRLLDPLLKPKAANCHHSINLFQKESVVTNKSGRSGNGKCSTILPEKELDKDQRFGCSTVNTAESSSEEKYIPSTSQALLRISMKNGQPLFTFAVGNNSNILAATVKYSTVPRQGECSCIYTFFTFREVKKKNGSRTNNTGKSKGPDYVRHVIAQMKLSDLHYDDSNSQNRVDSITKEFVLFSVKLKQGDTQVADYQPDDELAAIVVKSPKAISYTHQHECQDLLQVTVVLPSGVHSLPSDGGPSSLIERWKTGGSCDCGGWDLACKLKILANNNQTCRKSRTSEAYFADQYELFFQGTEQCQDNRPVFNFSHFEPGVYSVAFGSSLSPLQAFSICIAMVDGKLPYELSGSRNSIEGKNQAESLLVQTGELKGHGKSDDIPASYAAYPPVSPVGRV