AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052298

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052298.t1 MTR_4g076020 96.129 155 5 1 1 155 17 170 1.78e-104 306
MS.gene052298.t1 MTR_7g074650 50.000 84 39 1 72 155 441 521 1.43e-21 90.9
MS.gene052298.t1 MTR_1g069725 39.362 94 49 2 63 155 103 189 2.95e-14 69.7
MS.gene052298.t1 MTR_4g076140 35.185 108 65 2 50 153 30 136 9.33e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052298.t1 AT5G41920 61.538 104 39 1 53 155 27 130 9.17e-33 120
MS.gene052298.t1 AT3G54220 47.664 107 56 0 49 155 273 379 6.58e-23 94.7
MS.gene052298.t1 AT1G07530 36.667 90 54 1 66 155 393 479 8.18e-12 62.8

Find 40 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTACAGGTCTCAAACTTCT+AGG 0.268544 4.2:+33105317 MS.gene052298:CDS
GTTACCGATTTAGCAGTTAA+TGG 0.272210 4.2:-33105518 None:intergenic
AGATTCTTCTCCTTCGGTTT+CGG 0.352465 4.2:-33105297 None:intergenic
ACCTGTAGATTCTTCTCCTT+CGG 0.364116 4.2:-33105303 None:intergenic
CTCGCCATTAACTGCTAAAT+CGG 0.397282 4.2:+33105514 MS.gene052298:CDS
GTTCAGTTATCTCCGGTAAA+AGG 0.402006 4.2:-33105397 None:intergenic
ACTGAACTCTCTTCACCGTT+TGG 0.403561 4.2:+33105413 MS.gene052298:CDS
GAAGCCAGAGTGATGGTACT+AGG 0.454937 4.2:+33106856 MS.gene052298:CDS
GTGTTCGCGTTGAGCGCGTT+TGG 0.458235 4.2:-33105144 None:intergenic
CAACGGTGAAACCGAGTTGT+AGG 0.484579 4.2:-33105579 None:intergenic
ACAAGTCTTTGAACATGAAA+CGG 0.502255 4.2:+33105271 MS.gene052298:CDS
TTCACCGTTTGGAACTTCAC+CGG 0.509521 4.2:+33105424 MS.gene052298:CDS
CTACAACTCGGTTTCACCGT+TGG 0.539952 4.2:+33105580 MS.gene052298:CDS
CTACAGGTCTCAAACTTCTA+GGG 0.540758 4.2:+33105318 MS.gene052298:CDS
CGCGTTTCAATCCTACAACT+CGG 0.552518 4.2:+33105568 MS.gene052298:CDS
ATGGTACTAGGGAGGAGGTG+AGG 0.557602 4.2:+33106868 MS.gene052298:CDS
AAGCCAGAGTGATGGTACTA+GGG 0.564412 4.2:+33106857 MS.gene052298:CDS
GAAGAGAGTTCAGTTATCTC+CGG 0.569254 4.2:-33105404 None:intergenic
CGTGGTGGAACACGACGCAC+AGG 0.569793 4.2:+33105232 MS.gene052298:CDS
AAATACGCACCAACTCGCTC+CGG 0.573807 4.2:-33105443 None:intergenic
ACCGAAGGAGAAGAATCTAC+AGG 0.582225 4.2:+33105302 MS.gene052298:CDS
AAAGGTCGTTGGCGAAGTCG+AGG 0.582557 4.2:-33105379 None:intergenic
CGCTCCGGTGAAGTTCCAAA+CGG 0.584887 4.2:-33105428 None:intergenic
ATGTGCTGAGTGTGTTGCCA+TGG 0.593544 4.2:+33105352 MS.gene052298:CDS
GGAACTTCACCGGAGCGAGT+TGG 0.594130 4.2:+33105434 MS.gene052298:CDS
GAGTGATGGTACTAGGGAGG+AGG 0.594376 4.2:+33106863 MS.gene052298:CDS
GGCGAAGTCGAGGTTATCCA+TGG 0.595794 4.2:-33105369 None:intergenic
CTGTGCGTCGTGTTCCACCA+CGG 0.597321 4.2:-33105231 None:intergenic
CCTCCCTAGTACCATCACTC+TGG 0.598714 4.2:-33106860 None:intergenic
GAAACGGAAACCGAAACCGA+AGG 0.606138 4.2:+33105287 MS.gene052298:CDS
TATCTCCGGTAAAAGGTCGT+TGG 0.618446 4.2:-33105390 None:intergenic
GAAAGATGGTTCATGAACGT+TGG 0.619264 4.2:-33105180 None:intergenic
TGTTTAAGTCTCTGAATACG+TGG 0.621518 4.2:+33106833 MS.gene052298:intron
CGTGTGGTGAGTTCTTGTCT+CGG 0.629592 4.2:+33105485 MS.gene052298:CDS
TACGTGGGAAGCCAGAGTGA+TGG 0.632636 4.2:+33106849 MS.gene052298:CDS
CCAGAGTGATGGTACTAGGG+AGG 0.646894 4.2:+33106860 MS.gene052298:CDS
ACAAGCGTTACAAGCGCGTG+TGG 0.650613 4.2:+33105469 MS.gene052298:CDS
GTTTAAGTCTCTGAATACGT+GGG 0.676261 4.2:+33106834 MS.gene052298:intron
CTCTAGTGAGAAAACCGTGG+TGG 0.676760 4.2:+33105217 MS.gene052298:CDS
CTACTCTAGTGAGAAAACCG+TGG 0.700727 4.2:+33105214 MS.gene052298:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATTTTAAAAAATTATA+AGG - chr4.2:33106767-33106786 None:intergenic 0.0%
!! TATAATAATCGTTAATTGAT+TGG + chr4.2:33106786-33106805 MS.gene052298:intron 15.0%
! ACATAAGCAATTAAATCAGA+CGG - chr4.2:33106421-33106440 None:intergenic 25.0%
! CATTGTTTGTATGATATAAC+AGG + chr4.2:33105944-33105963 MS.gene052298:intron 25.0%
! TGAAACTCAAAGAAAAGAAA+AGG + chr4.2:33106664-33106683 MS.gene052298:intron 25.0%
! TGATATCAAGATCAATGATA+TGG - chr4.2:33105670-33105689 None:intergenic 25.0%
!! AATTGATTGGTTAAGAACAA+TGG + chr4.2:33106799-33106818 MS.gene052298:intron 25.0%
ACAAGTCTTTGAACATGAAA+CGG + chr4.2:33105271-33105290 MS.gene052298:CDS 30.0%
ATTGATCTTGATATCATGCA+AGG + chr4.2:33105674-33105693 MS.gene052298:intron 30.0%
CAAAGAAAAGAAAAGGACAT+AGG + chr4.2:33106671-33106690 MS.gene052298:intron 30.0%
TGAGTGTACATTTGATATCA+CGG + chr4.2:33106445-33106464 MS.gene052298:intron 30.0%
! ATGATTCACCTTGATTTTAC+CGG + chr4.2:33106533-33106552 MS.gene052298:intron 30.0%
! TGATGTTCATAGTTTGTCTT+TGG + chr4.2:33106587-33106606 MS.gene052298:intron 30.0%
!! GAACATCATCAAGTTGAATT+CGG - chr4.2:33106575-33106594 None:intergenic 30.0%
!! TAGTTAGGTCTTTTGAAAGA+TGG - chr4.2:33105197-33105216 None:intergenic 30.0%
!!! CGCAATGATCATGATTTTTT+TGG - chr4.2:33106479-33106498 None:intergenic 30.0%
!!! TGATCATGATTTTTTTGGTC+TGG - chr4.2:33106474-33106493 None:intergenic 30.0%
AAGTGTGAGAATTTCACCAA+CGG - chr4.2:33105599-33105618 None:intergenic 35.0%
AGAAAAGAAAAGGACATAGG+TGG + chr4.2:33106674-33106693 MS.gene052298:intron 35.0%
ATAAGGAACATTATCGCTGT+TGG + chr4.2:33106178-33106197 MS.gene052298:intron 35.0%
ATATCATGCAAGGACTTCAA+TGG + chr4.2:33105684-33105703 MS.gene052298:intron 35.0%
ATTTCGACCCGTTTCATTAA+GGG + chr4.2:33106264-33106283 MS.gene052298:intron 35.0%
GATATAACAGGAAGCGATTT+AGG + chr4.2:33105956-33105975 MS.gene052298:intron 35.0%
GTTTAAGTCTCTGAATACGT+GGG + chr4.2:33106834-33106853 MS.gene052298:intron 35.0%
TATACATTGGTGGAAGAGAA+TGG + chr4.2:33106340-33106359 MS.gene052298:intron 35.0%
TGTTTAAGTCTCTGAATACG+TGG + chr4.2:33106833-33106852 MS.gene052298:intron 35.0%
! AATTGGAAGTGTAACTGAAC+CGG + chr4.2:33105871-33105890 MS.gene052298:intron 35.0%
! CTTCCAATTTTACCCTCAAT+CGG - chr4.2:33105860-33105879 None:intergenic 35.0%
! GTTTTCTCACTAGAGTAGTT+AGG - chr4.2:33105212-33105231 None:intergenic 35.0%
! TGCGAATCAAGCAATTTTTC+AGG + chr4.2:33105622-33105641 MS.gene052298:intron 35.0%
! TGTTCATAGTTTGTCTTTGG+TGG + chr4.2:33106590-33106609 MS.gene052298:intron 35.0%
!! AGTTGAATTCGGTGTTTCTT+TGG - chr4.2:33106564-33106583 None:intergenic 35.0%
!! GTTACCGATTTAGCAGTTAA+TGG - chr4.2:33105521-33105540 None:intergenic 35.0%
!!! TCAAGCAATTTTTCAGGCTT+TGG + chr4.2:33105628-33105647 MS.gene052298:intron 35.0%
ACCAATGTATATCCTCTCCA+AGG - chr4.2:33106331-33106350 None:intergenic 40.0%
ACCTGTAGATTCTTCTCCTT+CGG - chr4.2:33105306-33105325 None:intergenic 40.0%
AGATTCTTCTCCTTCGGTTT+CGG - chr4.2:33105300-33105319 None:intergenic 40.0%
ATGGCTAAGATCCTGTTCAA+CGG - chr4.2:33106026-33106045 None:intergenic 40.0%
CATCCGATTGAGGGTAAAAT+TGG + chr4.2:33105854-33105873 MS.gene052298:intron 40.0%
CTACAGGTCTCAAACTTCTA+GGG + chr4.2:33105318-33105337 MS.gene052298:CDS 40.0%
CTCGCCATTAACTGCTAAAT+CGG + chr4.2:33105514-33105533 MS.gene052298:CDS 40.0%
GAAAGATGGTTCATGAACGT+TGG - chr4.2:33105183-33105202 None:intergenic 40.0%
GAAGAGAGTTCAGTTATCTC+CGG - chr4.2:33105407-33105426 None:intergenic 40.0%
GATTTCGACCCGTTTCATTA+AGG + chr4.2:33106263-33106282 MS.gene052298:intron 40.0%
GTGTACATTTGATATCACGG+TGG + chr4.2:33106448-33106467 MS.gene052298:intron 40.0%
GTTCAGTTATCTCCGGTAAA+AGG - chr4.2:33105400-33105419 None:intergenic 40.0%
TAATGAAACGGGTCGAAATC+CGG - chr4.2:33106263-33106282 None:intergenic 40.0%
TCAACGGTCGTGATCAATTT+CGG - chr4.2:33106010-33106029 None:intergenic 40.0%
TCAATCGGATGAAACTCGAA+CGG - chr4.2:33105845-33105864 None:intergenic 40.0%
TCCTTGGAGAGGATATACAT+TGG + chr4.2:33106327-33106346 MS.gene052298:intron 40.0%
TCTACAGGTCTCAAACTTCT+AGG + chr4.2:33105317-33105336 MS.gene052298:CDS 40.0%
TGAAGTGAAAGTTGAGAGGT+GGG + chr4.2:33106219-33106238 MS.gene052298:intron 40.0%
TTGGAGAGGATATACATTGG+TGG + chr4.2:33106330-33106349 MS.gene052298:intron 40.0%
TTTCGACCCGTTTCATTAAG+GGG + chr4.2:33106265-33106284 MS.gene052298:intron 40.0%
! ATTGGAAGTGTAACTGAACC+GGG + chr4.2:33105872-33105891 MS.gene052298:intron 40.0%
! CTTTGTTGATTGCTTCTGCT+TGG + chr4.2:33106392-33106411 MS.gene052298:intron 40.0%
! TTCGAGTTTCATCCGATTGA+GGG + chr4.2:33105845-33105864 MS.gene052298:intron 40.0%
!!! GCAATTTTTCAGGCTTTGGA+TGG + chr4.2:33105632-33105651 MS.gene052298:intron 40.0%
AAAATCGGCGAGTCTTCTTC+CGG - chr4.2:33105804-33105823 None:intergenic 45.0%
AAGCCAGAGTGATGGTACTA+GGG + chr4.2:33106857-33106876 MS.gene052298:CDS 45.0%
AAGTGAAAGTTGAGAGGTGG+GGG + chr4.2:33106221-33106240 MS.gene052298:intron 45.0%
ACCGAAGGAGAAGAATCTAC+AGG + chr4.2:33105302-33105321 MS.gene052298:CDS 45.0%
ACTGAACTCTCTTCACCGTT+TGG + chr4.2:33105413-33105432 MS.gene052298:CDS 45.0%
AGGAACATTATCGCTGTTGG+TGG + chr4.2:33106181-33106200 MS.gene052298:intron 45.0%
ATGAGGCAATCGTGGTTCAT+TGG + chr4.2:33105915-33105934 MS.gene052298:intron 45.0%
ATGGTTCTCTCAAGCTTGGT+TGG + chr4.2:33106359-33106378 MS.gene052298:intron 45.0%
ATTGATCACGACCGTTGAAC+AGG + chr4.2:33106012-33106031 MS.gene052298:intron 45.0%
CGCGTTTCAATCCTACAACT+CGG + chr4.2:33105568-33105587 MS.gene052298:CDS 45.0%
CGGGTTACCCCTTAATGAAA+CGG - chr4.2:33106275-33106294 None:intergenic 45.0%
CGTTTCATTAAGGGGTAACC+CGG + chr4.2:33106273-33106292 MS.gene052298:intron 45.0%
CTACTCTAGTGAGAAAACCG+TGG + chr4.2:33105214-33105233 MS.gene052298:CDS 45.0%
CTGGTGAAGTGAAAGTTGAG+AGG + chr4.2:33106215-33106234 MS.gene052298:intron 45.0%
GAAGTGAAAGTTGAGAGGTG+GGG + chr4.2:33106220-33106239 MS.gene052298:intron 45.0%
GAGAATGGTTCTCTCAAGCT+TGG + chr4.2:33106355-33106374 MS.gene052298:intron 45.0%
GGAACATTATCGCTGTTGGT+GGG + chr4.2:33106182-33106201 MS.gene052298:intron 45.0%
GGGTTACCCCTTAATGAAAC+GGG - chr4.2:33106274-33106293 None:intergenic 45.0%
GTGAAGTGAAAGTTGAGAGG+TGG + chr4.2:33106218-33106237 MS.gene052298:intron 45.0%
TAGCTTCTTAGCGAGGTTTG+TGG + chr4.2:33106051-33106070 MS.gene052298:intron 45.0%
TATCTCCGGTAAAAGGTCGT+TGG - chr4.2:33105393-33105412 None:intergenic 45.0%
TGAACCACGATTGCCTCATT+CGG - chr4.2:33105914-33105933 None:intergenic 45.0%
TGTTCCACATCCTTGCTTCA+CGG + chr4.2:33105714-33105733 MS.gene052298:intron 45.0%
TGTTGTTGGGGATGTTTCCT+TGG + chr4.2:33106311-33106330 MS.gene052298:intron 45.0%
TTCACTTCACCAGTCCTCTT+AGG - chr4.2:33106208-33106227 None:intergenic 45.0%
TTCTCTCAAGCTTGGTTGGA+AGG + chr4.2:33106363-33106382 MS.gene052298:intron 45.0%
! GGATAGTGTTGAGAGGCATA+TGG + chr4.2:33106129-33106148 MS.gene052298:intron 45.0%
! GTTCGAGTTTCATCCGATTG+AGG + chr4.2:33105844-33105863 MS.gene052298:intron 45.0%
! TAGTGTTGAGAGGCATATGG+TGG + chr4.2:33106132-33106151 MS.gene052298:intron 45.0%
! TGGTCTTGCCGGTAAAATCA+AGG - chr4.2:33106544-33106563 None:intergenic 45.0%
! TTCACCGTTTGGAACTTCAC+CGG + chr4.2:33105424-33105443 MS.gene052298:CDS 45.0%
!! TCGGTGAGAATTACCGGTTT+CGG + chr4.2:33105752-33105771 MS.gene052298:intron 45.0%
!! TCTCAGGCGAGTTTGTTGTT+GGG + chr4.2:33106298-33106317 MS.gene052298:intron 45.0%
!! TTCTCAGGCGAGTTTGTTGT+TGG + chr4.2:33106297-33106316 MS.gene052298:intron 45.0%
AAATACGCACCAACTCGCTC+CGG - chr4.2:33105446-33105465 None:intergenic 50.0%
AACAAACTCGCCTGAGAAGC+CGG - chr4.2:33106295-33106314 None:intergenic 50.0%
AACCGGTAATTCTCACCGAG+CGG - chr4.2:33105751-33105770 None:intergenic 50.0%
AGCTGTTGGGGTGTGAGATA+AGG + chr4.2:33106161-33106180 MS.gene052298:intron 50.0%
AGTGAAAGTTGAGAGGTGGG+GGG + chr4.2:33106222-33106241 MS.gene052298:intron 50.0%
ATGCAAGGACTTCAATGGCC+AGG + chr4.2:33105689-33105708 MS.gene052298:intron 50.0%
ATGCTGGTAGCTTCTTAGCG+AGG + chr4.2:33106044-33106063 MS.gene052298:intron 50.0%
ATGTGCTGAGTGTGTTGCCA+TGG + chr4.2:33105352-33105371 MS.gene052298:CDS 50.0%
CAACGGTGAAACCGAGTTGT+AGG - chr4.2:33105582-33105601 None:intergenic 50.0%
CGTGTGGTGAGTTCTTGTCT+CGG + chr4.2:33105485-33105504 MS.gene052298:CDS 50.0%
CTACAACTCGGTTTCACCGT+TGG + chr4.2:33105580-33105599 MS.gene052298:CDS 50.0%
CTCGGAAGAAGATCCGAAAC+CGG - chr4.2:33105768-33105787 None:intergenic 50.0%
CTCTAGTGAGAAAACCGTGG+TGG + chr4.2:33105217-33105236 MS.gene052298:CDS 50.0%
CTTCTTAGCGAGGTTTGTGG+AGG + chr4.2:33106054-33106073 MS.gene052298:intron 50.0%
GAAACGGAAACCGAAACCGA+AGG + chr4.2:33105287-33105306 MS.gene052298:CDS 50.0%
GAACAGGATCTTAGCCATGC+TGG + chr4.2:33106028-33106047 MS.gene052298:intron 50.0%
GAAGCCAGAGTGATGGTACT+AGG + chr4.2:33106856-33106875 MS.gene052298:CDS 50.0%
GCAAGGATGTGGAACAATCC+TGG - chr4.2:33105710-33105729 None:intergenic 50.0%
GTAACTGAACCGGGTCAACT+CGG + chr4.2:33105881-33105900 MS.gene052298:intron 50.0%
TAGACCGAATGAGGCAATCG+TGG + chr4.2:33105907-33105926 MS.gene052298:intron 50.0%
TCCGAGCTGTTAGAACCAAC+CGG + chr4.2:33105782-33105801 MS.gene052298:intron 50.0%
TCGCTAAGAAGCTACCAGCA+TGG - chr4.2:33106045-33106064 None:intergenic 50.0%
TCTTCTTCGACCGTGAAGCA+AGG - chr4.2:33105727-33105746 None:intergenic 50.0%
TTGGGGATGTTTCCTTGGAG+AGG + chr4.2:33106316-33106335 MS.gene052298:intron 50.0%
! ATCCGCTCGGTGAGAATTAC+CGG + chr4.2:33105746-33105765 MS.gene052298:intron 50.0%
! CTTCGCCAACGACCTTTTAC+CGG + chr4.2:33105385-33105404 MS.gene052298:CDS 50.0%
! TGGGTGTGGATAGTGTTGAG+AGG + chr4.2:33106122-33106141 MS.gene052298:intron 50.0%
!! ACTCGGTGTTAGACCGAATG+AGG + chr4.2:33105898-33105917 MS.gene052298:intron 50.0%
!! CGGTGTTTCTTTGGTCTTGC+CGG - chr4.2:33106555-33106574 None:intergenic 50.0%
!! CTCAGGCGAGTTTGTTGTTG+GGG + chr4.2:33106299-33106318 MS.gene052298:intron 50.0%
!! TCCGGTTGGTTCTAACAGCT+CGG - chr4.2:33105786-33105805 None:intergenic 50.0%
!! TGAGATTGCTGACTCAGCTG+AGG + chr4.2:33105984-33106003 MS.gene052298:intron 50.0%
!! TTATTCGACGCTCTAGGGGA+TGG + chr4.2:33106097-33106116 MS.gene052298:intron 50.0%
AAAGGTCGTTGGCGAAGTCG+AGG - chr4.2:33105382-33105401 None:intergenic 55.0%
ACAAACTCGCCTGAGAAGCC+GGG - chr4.2:33106294-33106313 None:intergenic 55.0%
ACAAGCGTTACAAGCGCGTG+TGG + chr4.2:33105469-33105488 MS.gene052298:CDS 55.0%
ACGGTCGAAGAAGATCCGCT+CGG + chr4.2:33105733-33105752 MS.gene052298:intron 55.0%
AGCGCGTTATTCGACGCTCT+AGG + chr4.2:33106091-33106110 MS.gene052298:intron 55.0%
CCAGAGTGATGGTACTAGGG+AGG + chr4.2:33106860-33106879 MS.gene052298:CDS 55.0%
CCCGAGCGAACTCGCAAAAT+CGG - chr4.2:33105819-33105838 None:intergenic 55.0%
CCTCCCTAGTACCATCACTC+TGG - chr4.2:33106863-33106882 None:intergenic 55.0%
CGCGTTATTCGACGCTCTAG+GGG + chr4.2:33106093-33106112 MS.gene052298:intron 55.0%
CGCTCCGGTGAAGTTCCAAA+CGG - chr4.2:33105431-33105450 None:intergenic 55.0%
GAGTGATGGTACTAGGGAGG+AGG + chr4.2:33106863-33106882 MS.gene052298:CDS 55.0%
GCGCGTTATTCGACGCTCTA+GGG + chr4.2:33106092-33106111 MS.gene052298:intron 55.0%
GGCGAAGTCGAGGTTATCCA+TGG - chr4.2:33105372-33105391 None:intergenic 55.0%
TACGTGGGAAGCCAGAGTGA+TGG + chr4.2:33106849-33106868 MS.gene052298:CDS 55.0%
TCGACCGTGAAGCAAGGATG+TGG - chr4.2:33105721-33105740 None:intergenic 55.0%
! ATGGTGGAGCAGCAGCTGTT+GGG + chr4.2:33106148-33106167 MS.gene052298:intron 55.0%
! CCGATTTTGCGAGTTCGCTC+GGG + chr4.2:33105816-33105835 MS.gene052298:intron 55.0%
! GGTCTAACACCGAGTTGACC+CGG - chr4.2:33105893-33105912 None:intergenic 55.0%
! GGTGGGGGGATGAGTTGAAA+CGG + chr4.2:33106236-33106255 MS.gene052298:intron 55.0%
! GTGGGGGGATGAGTTGAAAC+GGG + chr4.2:33106237-33106256 MS.gene052298:intron 55.0%
! TATGGTGGAGCAGCAGCTGT+TGG + chr4.2:33106147-33106166 MS.gene052298:intron 55.0%
! TCTAGGGGATGGTTTGGGTG+TGG + chr4.2:33106108-33106127 MS.gene052298:intron 55.0%
!! GACGCTCTAGGGGATGGTTT+GGG + chr4.2:33106103-33106122 MS.gene052298:intron 55.0%
!! GCCGATTTTGCGAGTTCGCT+CGG + chr4.2:33105815-33105834 MS.gene052298:intron 55.0%
AAGGGGTAACCCGGCTTCTC+AGG + chr4.2:33106282-33106301 MS.gene052298:intron 60.0%
CTGTGCGTCGTGTTCCACCA+CGG - chr4.2:33105234-33105253 None:intergenic 60.0%
GGAACTTCACCGGAGCGAGT+TGG + chr4.2:33105434-33105453 MS.gene052298:CDS 60.0%
GTGTTCGCGTTGAGCGCGTT+TGG - chr4.2:33105147-33105166 None:intergenic 60.0%
TCGGCGAGTCTTCTTCCGGT+TGG - chr4.2:33105800-33105819 None:intergenic 60.0%
TGGTGGAGCAGCAGCTGTTG+GGG + chr4.2:33106149-33106168 MS.gene052298:intron 60.0%
! GGGGATGAGTTGAAACGGGC+CGG + chr4.2:33106241-33106260 MS.gene052298:intron 60.0%
! GTTGGTGGGCCTAAGAGGAC+TGG + chr4.2:33106196-33106215 MS.gene052298:intron 60.0%
! TCGCTGTTGGTGGGCCTAAG+AGG + chr4.2:33106191-33106210 MS.gene052298:intron 60.0%
!! CGACGCTCTAGGGGATGGTT+TGG + chr4.2:33106102-33106121 MS.gene052298:intron 60.0%
! CGTGGTGGAACACGACGCAC+AGG + chr4.2:33105232-33105251 MS.gene052298:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 33105137 33106888 33105137 ID=MS.gene052298
chr4.2 mRNA 33105137 33106888 33105137 ID=MS.gene052298.t1;Parent=MS.gene052298
chr4.2 exon 33105137 33105601 33105137 ID=MS.gene052298.t1.exon1;Parent=MS.gene052298.t1
chr4.2 CDS 33105137 33105601 33105137 ID=cds.MS.gene052298.t1;Parent=MS.gene052298.t1
chr4.2 exon 33106841 33106888 33106841 ID=MS.gene052298.t1.exon2;Parent=MS.gene052298.t1
chr4.2 CDS 33106841 33106888 33106841 ID=cds.MS.gene052298.t1;Parent=MS.gene052298.t1
Gene Sequence

>MS.gene052298

ATGAACACCAAACGCGCTCAACGCGAACACGATGACGACGACACCAACGTTCATGAACCATCTTTCAAAAGACCTAACTACTCTAGTGAGAAAACCGTGGTGGAACACGACGCACAGGACGACGAACAACAACAACAAGTCTTTGAACATGAAACGGAAACCGAAACCGAAGGAGAAGAATCTACAGGTCTCAAACTTCTAGGGCTTTTACTTCAATGTGCTGAGTGTGTTGCCATGGATAACCTCGACTTCGCCAACGACCTTTTACCGGAGATAACTGAACTCTCTTCACCGTTTGGAACTTCACCGGAGCGAGTTGGTGCGTATTTTGCACAAGCGTTACAAGCGCGTGTGGTGAGTTCTTGTCTCGGAAGCTACTCGCCATTAACTGCTAAATCGGTAACGCTAAATCAGTCTCAGAGAATCTTCAACGCGTTTCAATCCTACAACTCGGTTTCACCGTTGGTGAAATTCTCACACTTCACTGCGAATCAAGCAATTTTTCAGGCTTTGGATGGTGAAGATCGTGTCCATATCATTGATCTTGATATCATGCAAGGACTTCAATGGCCAGGATTGTTCCACATCCTTGCTTCACGGTCGAAGAAGATCCGCTCGGTGAGAATTACCGGTTTCGGATCTTCTTCCGAGCTGTTAGAACCAACCGGAAGAAGACTCGCCGATTTTGCGAGTTCGCTCGGGTTACCGTTCGAGTTTCATCCGATTGAGGGTAAAATTGGAAGTGTAACTGAACCGGGTCAACTCGGTGTTAGACCGAATGAGGCAATCGTGGTTCATTGGATGCATCATTGTTTGTATGATATAACAGGAAGCGATTTAGGAACGTTGAGATTGCTGACTCAGCTGAGGCCGAAATTGATCACGACCGTTGAACAGGATCTTAGCCATGCTGGTAGCTTCTTAGCGAGGTTTGTGGAGGCTTTGCATTATTACAGCGCGTTATTCGACGCTCTAGGGGATGGTTTGGGTGTGGATAGTGTTGAGAGGCATATGGTGGAGCAGCAGCTGTTGGGGTGTGAGATAAGGAACATTATCGCTGTTGGTGGGCCTAAGAGGACTGGTGAAGTGAAAGTTGAGAGGTGGGGGGATGAGTTGAAACGGGCCGGATTTCGACCCGTTTCATTAAGGGGTAACCCGGCTTCTCAGGCGAGTTTGTTGTTGGGGATGTTTCCTTGGAGAGGATATACATTGGTGGAAGAGAATGGTTCTCTCAAGCTTGGTTGGAAGGATCTCTCTTTGTTGATTGCTTCTGCTTGGCAGCCGTCTGATTTAATTGCTTATGTCGATTGAGTGTACATTTGATATCACGGTGGCCAGACCAAAAAAATCATGATCATTGCGTGATTTTGTTGAAGCAATGTTTTACAAAGTTACGATGATTCACCTTGATTTTACCGGCAAGACCAAAGAAACACCGAATTCAACTTGATGATGTTCATAGTTTGTCTTTGGTGGTAGAGCAACTGTTGATTAATTTCTCTCATGCTTATGCAAAGTCTCTTGTCTTGAAACTCAAAGAAAAGAAAAGGACATAGGTGGTAGAGAATAGAATCTTTTAAATTAGTGAAGTGTCATAAGTGAGAAGTCATTCACATTGTCAAATGTACCTTATAATTTTTTAAAATTTTTATAATAATCGTTAATTGATTGGTTAAGAACAATGGATTATTGTCTGTGTTTAAGTCTCTGAATACGTGGGAAGCCAGAGTGATGGTACTAGGGAGGAGGTGA
Protein sequence

>MS.gene052298.t1

MNTKRAQREHDDDDTNVHEPSFKRPNYSSEKTVVEHDAQDDEQQQQVFEHETETETEGEESTGLKLLGLLLQCAECVAMDNLDFANDLLPEITELSSPFGTSPERVGAYFAQALQARVVSSCLGSYSPLTAKSVTLNQSQRIFNAFQSYNSVSPLSLNTWEARVMVLGRR