AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28932

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28932.t1 MTR_4g076020 89.286 308 20 1 1 308 17 311 0.0 509
MS.gene28932.t1 MTR_7g074650 60.504 238 90 2 71 308 441 674 1.53e-95 300
MS.gene28932.t1 MTR_1g069725 51.417 247 112 2 62 307 103 342 3.92e-82 256
MS.gene28932.t1 MTR_7g027190 34.694 294 166 6 42 312 267 557 9.93e-48 170
MS.gene28932.t1 MTR_4g102790 36.719 256 155 4 62 311 286 540 1.55e-44 161
MS.gene28932.t1 MTR_3g065980 33.591 259 155 4 59 311 163 410 9.80e-39 144
MS.gene28932.t1 MTR_4g064150 29.927 274 180 3 50 311 343 616 3.41e-36 138
MS.gene28932.t1 MTR_5g058860 35.955 267 150 7 53 300 104 368 8.88e-34 129
MS.gene28932.t1 MTR_3g072710 36.559 279 152 9 52 307 104 380 1.12e-33 129
MS.gene28932.t1 MTR_4g104020 30.686 277 178 6 49 312 130 405 2.22e-33 128
MS.gene28932.t1 MTR_1g029420 35.271 258 149 7 68 312 225 477 3.43e-33 129
MS.gene28932.t1 MTR_2g097410 28.159 277 173 4 53 311 356 624 4.43e-33 129
MS.gene28932.t1 MTR_5g009080 32.524 206 136 2 59 264 47 249 1.33e-32 126
MS.gene28932.t1 MTR_4g064120 26.316 285 198 3 39 311 225 509 2.61e-31 123
MS.gene28932.t1 MTR_3g089055 35.039 254 146 8 68 307 198 446 4.56e-31 122
MS.gene28932.t1 MTR_3g089055 35.039 254 146 8 68 307 203 451 5.38e-31 122
MS.gene28932.t1 MTR_4g064180 27.778 288 190 6 39 311 225 509 1.93e-30 121
MS.gene28932.t1 MTR_5g094450 33.061 245 150 5 68 299 161 404 5.02e-30 119
MS.gene28932.t1 MTR_4g076140 33.810 210 128 3 59 264 39 241 7.11e-30 118
MS.gene28932.t1 MTR_4g077760 34.000 250 143 7 65 301 190 430 4.16e-29 117
MS.gene28932.t1 MTR_8g442410 31.864 295 189 4 30 312 117 411 7.84e-29 116
MS.gene28932.t1 MTR_2g082090 32.061 262 160 6 50 299 196 451 1.01e-28 116
MS.gene28932.t1 MTR_5g097480 33.197 244 147 5 68 299 177 416 2.30e-28 114
MS.gene28932.t1 MTR_4g064200 29.434 265 166 6 60 311 276 532 2.51e-28 115
MS.gene28932.t1 MTR_4g097080 30.081 246 153 7 67 299 132 371 3.08e-28 114
MS.gene28932.t1 MTR_2g099110 30.864 243 151 7 68 299 74 310 3.90e-28 113
MS.gene28932.t1 MTR_1g096030 33.333 255 145 8 58 299 70 312 5.39e-28 113
MS.gene28932.t1 MTR_8g093070 32.812 256 151 7 52 300 125 366 1.17e-27 112
MS.gene28932.t1 MTR_3g056110 30.000 260 166 5 65 312 172 427 2.21e-27 112
MS.gene28932.t1 MTR_5g015490 28.958 259 161 7 59 299 105 358 3.36e-27 111
MS.gene28932.t1 MTR_4g064160 27.368 285 191 5 39 311 287 567 6.88e-27 111
MS.gene28932.t1 MTR_2g097467 26.452 310 188 8 27 311 243 537 7.20e-27 110
MS.gene28932.t1 MTR_2g097310 30.660 212 137 4 63 272 267 470 1.30e-26 110
MS.gene28932.t1 MTR_7g062120 29.600 250 146 7 63 293 367 605 2.13e-26 109
MS.gene28932.t1 MTR_2g097350 32.195 205 133 3 63 265 269 469 6.02e-26 108
MS.gene28932.t1 MTR_2g097473 28.358 268 165 6 60 311 281 537 7.49e-26 108
MS.gene28932.t1 MTR_2g097463 28.358 268 165 6 60 311 282 538 1.50e-25 107
MS.gene28932.t1 MTR_3g022830 31.122 196 132 2 69 264 26 218 1.70e-25 105
MS.gene28932.t1 MTR_4g133660 31.200 250 155 6 63 299 170 415 4.40e-24 102
MS.gene28932.t1 MTR_2g097390 29.902 204 137 3 63 264 270 469 6.88e-24 102
MS.gene28932.t1 MTR_2g034280 29.213 267 177 3 58 312 193 459 2.00e-23 100
MS.gene28932.t1 MTR_2g034260 29.670 273 170 6 58 312 200 468 2.78e-23 100
MS.gene28932.t1 MTR_0092s0100 30.932 236 150 6 69 298 376 604 2.36e-22 97.8
MS.gene28932.t1 MTR_0092s0100 30.932 236 150 6 69 298 373 601 2.53e-22 97.8
MS.gene28932.t1 MTR_2g089100 24.460 278 175 5 68 311 52 328 5.12e-22 96.3
MS.gene28932.t1 MTR_2g026250 28.458 253 158 7 63 299 223 468 1.43e-21 95.5
MS.gene28932.t1 MTR_4g026485 29.339 242 149 6 69 299 270 500 2.03e-21 95.1
MS.gene28932.t1 MTR_2g034250 27.473 273 176 6 58 312 200 468 8.54e-21 93.2
MS.gene28932.t1 MTR_2g097380 28.378 222 135 5 53 272 209 408 1.05e-20 92.8
MS.gene28932.t1 MTR_7g109580 25.660 265 182 4 58 312 179 438 6.00e-19 87.4
MS.gene28932.t1 MTR_1g086970 22.388 268 194 6 58 312 96 362 4.72e-13 69.7
MS.gene28932.t1 MTR_8g077940 31.176 170 109 5 69 234 183 348 8.88e-13 68.9
MS.gene28932.t1 MTR_1g106590 30.000 120 82 1 62 181 124 241 1.38e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28932.t1 AT5G41920 69.231 260 77 2 52 308 27 286 1.22e-124 362
MS.gene28932.t1 AT3G54220 57.196 271 113 2 39 308 264 532 9.21e-98 301
MS.gene28932.t1 AT1G63100 35.827 254 157 4 63 311 247 499 1.48e-39 147
MS.gene28932.t1 AT1G63100 35.827 254 157 4 63 311 272 524 2.18e-39 146
MS.gene28932.t1 AT3G03450 34.615 260 158 5 59 311 174 428 1.51e-37 140
MS.gene28932.t1 AT1G66350 33.984 256 157 4 59 311 146 392 1.13e-34 132
MS.gene28932.t1 AT1G50420 35.437 206 132 1 59 264 48 252 1.62e-33 128
MS.gene28932.t1 AT5G17490 32.692 260 157 6 59 311 151 399 1.30e-32 126
MS.gene28932.t1 AT2G01570 30.268 261 165 5 59 311 215 466 4.15e-30 120
MS.gene28932.t1 AT1G14920 31.679 262 160 5 59 311 163 414 1.88e-29 117
MS.gene28932.t1 AT1G07520 27.306 271 177 5 58 311 144 411 2.47e-28 114
MS.gene28932.t1 AT1G07520 27.306 271 177 5 58 311 144 411 2.47e-28 114
MS.gene28932.t1 AT1G07520 27.306 271 177 5 58 311 309 576 3.19e-28 115
MS.gene28932.t1 AT4G37650 29.572 257 156 7 68 304 146 397 3.22e-28 114
MS.gene28932.t1 AT1G07520 27.306 271 177 5 58 311 308 575 3.40e-28 115
MS.gene28932.t1 AT1G07530 29.615 260 166 6 65 311 393 648 4.79e-28 114
MS.gene28932.t1 AT2G04890 33.333 243 149 6 63 299 50 285 6.21e-28 112
MS.gene28932.t1 AT5G48150 31.687 243 151 5 69 299 123 362 9.28e-28 112
MS.gene28932.t1 AT5G48150 31.687 243 151 5 69 299 123 362 9.28e-28 112
MS.gene28932.t1 AT5G48150 31.687 243 151 5 69 299 123 362 9.28e-28 112
MS.gene28932.t1 AT5G48150 31.687 243 151 5 69 299 123 362 9.28e-28 112
MS.gene28932.t1 AT5G59450 27.636 275 180 5 51 311 210 479 1.72e-27 112
MS.gene28932.t1 AT5G66770 33.852 257 151 8 65 311 217 464 1.97e-27 112
MS.gene28932.t1 AT1G50600 33.198 247 149 5 65 299 156 398 3.02e-27 111
MS.gene28932.t1 AT1G21450 31.008 258 160 6 68 312 225 477 4.44e-27 111
MS.gene28932.t1 AT1G50600 33.198 247 149 5 65 299 231 473 6.29e-27 111
MS.gene28932.t1 AT1G50600 33.198 247 149 5 65 299 227 469 6.85e-27 110
MS.gene28932.t1 AT3G50650 34.322 236 135 6 86 311 193 418 7.26e-27 110
MS.gene28932.t1 AT2G29060 33.679 193 115 4 65 248 281 469 1.23e-25 107
MS.gene28932.t1 AT2G29060 33.679 193 115 4 65 248 318 506 1.65e-25 107
MS.gene28932.t1 AT2G37650 28.352 261 169 6 63 311 330 584 2.32e-25 106
MS.gene28932.t1 AT2G37650 28.352 261 169 6 63 311 342 596 2.32e-25 106
MS.gene28932.t1 AT4G17230 32.061 262 162 5 63 312 153 410 3.82e-25 105
MS.gene28932.t1 AT4G17230 32.061 262 162 5 63 312 153 410 3.82e-25 105
MS.gene28932.t1 AT3G46600 28.185 259 169 4 65 311 1 254 7.18e-25 103
MS.gene28932.t1 AT3G46600 27.969 261 171 4 63 311 207 462 9.08e-25 104
MS.gene28932.t1 AT3G46600 28.185 259 169 4 65 311 177 430 1.63e-24 103
MS.gene28932.t1 AT2G29065 28.462 260 169 5 68 311 252 510 3.03e-24 103
MS.gene28932.t1 AT4G08250 29.323 266 170 8 54 305 93 354 2.46e-23 100
MS.gene28932.t1 AT1G55580 32.624 282 147 11 68 307 44 324 5.12e-22 96.3
MS.gene28932.t1 AT4G00150 29.958 237 134 9 69 296 209 422 2.79e-16 79.7
MS.gene28932.t1 AT3G49950 27.306 271 164 10 68 311 30 294 4.60e-16 78.6
MS.gene28932.t1 AT3G60630 39.216 102 54 2 144 237 329 430 6.59e-14 72.8
MS.gene28932.t1 AT4G36710 25.869 259 174 9 59 306 116 367 1.75e-11 65.1

Find 72 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATATAACAGGAAGCGATTT+AGG 0.268170 4.4:+34439092 MS.gene28932:CDS
TCTACAGGTCTCAAACTTCT+AGG 0.268544 4.4:+34438453 MS.gene28932:CDS
GTTACCGATTTAGCAGTTAA+TGG 0.272210 4.4:-34438654 None:intergenic
CATCCGATTGAGGGTAAAAT+TGG 0.305802 4.4:+34438990 MS.gene28932:CDS
TCAACGGTCGTGATCAATTT+CGG 0.318610 4.4:-34439143 None:intergenic
TGATATCAAGATCAATGATA+TGG 0.341996 4.4:-34438803 None:intergenic
ATCCGCTCGGTGAGAATTAC+CGG 0.347135 4.4:+34438882 MS.gene28932:CDS
CATTGTTTGTATGATATAAC+AGG 0.349848 4.4:+34439080 MS.gene28932:CDS
AGATTCTTCTCCTTCGGTTT+CGG 0.352465 4.4:-34438433 None:intergenic
ACCTGTAGATTCTTCTCCTT+CGG 0.364116 4.4:-34438439 None:intergenic
ATTGATCACGACCGTTGAAC+AGG 0.382235 4.4:+34439148 MS.gene28932:CDS
AATTGGAAGTGTAACTGAAC+CGG 0.391845 4.4:+34439007 MS.gene28932:CDS
CTCGCCATTAACTGCTAAAT+CGG 0.397282 4.4:+34438650 MS.gene28932:CDS
GTTCAGTTATCTCCGGTAAA+AGG 0.402006 4.4:-34438533 None:intergenic
ACTGAACTCTCTTCACCGTT+TGG 0.403561 4.4:+34438549 MS.gene28932:CDS
CCCGAGCGAACTCGCAAAAT+CGG 0.412376 4.4:-34438952 None:intergenic
ATGAGGCAATCGTGGTTCAT+TGG 0.429274 4.4:+34439051 MS.gene28932:CDS
TCCGAGCTGTTAGAATCAAC+CGG 0.448871 4.4:+34438918 MS.gene28932:CDS
TTCGAGTTTCATCCGATTGA+GGG 0.456448 4.4:+34438981 MS.gene28932:CDS
GTGTTCGCGTTGAGCGCGTT+TGG 0.458235 4.4:-34438283 None:intergenic
TCGGTGAGAATTACCGGTTT+CGG 0.471801 4.4:+34438888 MS.gene28932:CDS
TGAACCACGATTGCCTCATT+CGG 0.484162 4.4:-34439047 None:intergenic
CAACGGTGAAACCGAGTTGT+AGG 0.484579 4.4:-34438715 None:intergenic
TGTTCCACATCCTTGCTTCG+AGG 0.493152 4.4:+34438850 MS.gene28932:CDS
GCAAGGATGTGGAACAATCC+TGG 0.495564 4.4:-34438843 None:intergenic
TCAAGTCTTTGAACATGAAA+CGG 0.502255 4.4:+34438407 MS.gene28932:CDS
AAAATCGGCGAGTCTTCTTC+CGG 0.502799 4.4:-34438937 None:intergenic
GTTCGAGTTTCATCCGATTG+AGG 0.506283 4.4:+34438980 MS.gene28932:CDS
TTCACCGTTTGGAACTTCAC+CGG 0.509521 4.4:+34438560 MS.gene28932:CDS
ATATCATGCAAGGACTTCAA+TGG 0.519599 4.4:+34438820 MS.gene28932:CDS
TCGACCTCGAAGCAAGGATG+TGG 0.523785 4.4:-34438854 None:intergenic
GAACGTTGAGATTGCTGACT+CGG 0.523872 4.4:+34439114 MS.gene28932:CDS
CTCGGAAGAAGATCCGAAAC+CGG 0.524772 4.4:-34438901 None:intergenic
TCCGGTTGATTCTAACAGCT+CGG 0.529075 4.4:-34438919 None:intergenic
GGTCTAACACCGAGTTGACC+CGG 0.536418 4.4:-34439026 None:intergenic
ATGCAAGGACTTCAATGGCC+AGG 0.537402 4.4:+34438825 MS.gene28932:CDS
TGAGATTGCTGACTCGGCTG+AGG 0.537567 4.4:+34439120 MS.gene28932:CDS
CTACAACTCGGTTTCACCGT+TGG 0.539952 4.4:+34438716 MS.gene28932:CDS
CTACAGGTCTCAAACTTCTA+GGG 0.540758 4.4:+34438454 MS.gene28932:CDS
TCTTCTTCGACCTCGAAGCA+AGG 0.546617 4.4:-34438860 None:intergenic
CGCGTTTCAATCCTACAACT+CGG 0.552518 4.4:+34438704 MS.gene28932:CDS
ACTCGGTGTTAGACCGAATG+AGG 0.563043 4.4:+34439034 MS.gene28932:CDS
GAAGAGAGTTCAGTTATCTC+CGG 0.569254 4.4:-34438540 None:intergenic
CGTGGTGGAACACGACGCAC+AGG 0.569793 4.4:+34438371 MS.gene28932:CDS
AAATACGCACCAACTCGCTC+CGG 0.573807 4.4:-34438579 None:intergenic
ACCGAAGGAGAAGAATCTAC+AGG 0.582225 4.4:+34438438 MS.gene28932:CDS
AAAGGTCGTTGGCGAAGTCG+AGG 0.582557 4.4:-34438515 None:intergenic
ATTGATCTTGATATCATGCA+AGG 0.582635 4.4:+34438810 MS.gene28932:CDS
ATTGGAAGTGTAACTGAACC+GGG 0.584733 4.4:+34439008 MS.gene28932:CDS
CGCTCCGGTGAAGTTCCAAA+CGG 0.584887 4.4:-34438564 None:intergenic
GAACAGGATCTTAGCCATGC+TGG 0.589797 4.4:+34439164 MS.gene28932:CDS
ATGTGCTGAGTGTGTTGCCA+TGG 0.593544 4.4:+34438488 MS.gene28932:CDS
GGAACTTCACCGGAGCGAGT+TGG 0.594130 4.4:+34438570 MS.gene28932:CDS
GGCGAAGTCGAGGTTATCCA+TGG 0.595794 4.4:-34438505 None:intergenic
CTGTGCGTCGTGTTCCACCA+CGG 0.597321 4.4:-34438370 None:intergenic
GAAACGGAAACCGAAACCGA+AGG 0.606138 4.4:+34438423 MS.gene28932:CDS
ATGCTGGTAGCTTCTTAGCG+AGG 0.608930 4.4:+34439180 MS.gene28932:CDS
TATCTCCGGTAAAAGGTCGT+TGG 0.618446 4.4:-34438526 None:intergenic
GAAAGATGGTTCATGAACGT+TGG 0.619264 4.4:-34438319 None:intergenic
ACATAAGCAATTAAATCAGA+CGG 0.619937 4.4:-34439554 None:intergenic
CGTGTGGTGAGTTCTTGTCT+CGG 0.629592 4.4:+34438621 MS.gene28932:CDS
GTAACTGAACCGGGTCAACT+CGG 0.642976 4.4:+34439017 MS.gene28932:CDS
ACAAGCGTTACAAGCGCGTG+TGG 0.650613 4.4:+34438605 MS.gene28932:CDS
ATGGCTAAGATCCTGTTCAA+CGG 0.673618 4.4:-34439159 None:intergenic
CTCTAGTGAGAAAACCGTGG+TGG 0.676760 4.4:+34438356 MS.gene28932:CDS
TCGCTAAGAAGCTACCAGCA+TGG 0.677504 4.4:-34439178 None:intergenic
TAGACCGAATGAGGCAATCG+TGG 0.677791 4.4:+34439043 MS.gene28932:CDS
AAGTGTGAGAATTTCACCAA+CGG 0.681780 4.4:-34438732 None:intergenic
GAGGTCGAAGAAGATCCGCT+CGG 0.688733 4.4:+34438869 MS.gene28932:CDS
AACCGGTAATTCTCACCGAG+CGG 0.697234 4.4:-34438884 None:intergenic
CTACTCTAGTGAGAAAACCG+TGG 0.700727 4.4:+34438353 MS.gene28932:CDS
TCAATCGGATGAAACTCGAA+CGG 0.709737 4.4:-34438978 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CATTGTTTGTATGATATAAC+AGG + chr4.4:34439080-34439099 MS.gene28932:CDS 25.0%
! TGATATCAAGATCAATGATA+TGG - chr4.4:34438806-34438825 None:intergenic 25.0%
ATTGATCTTGATATCATGCA+AGG + chr4.4:34438810-34438829 MS.gene28932:CDS 30.0%
TCAAGTCTTTGAACATGAAA+CGG + chr4.4:34438407-34438426 MS.gene28932:CDS 30.0%
!! TAGTTAGGTCTTTTGAAAGA+TGG - chr4.4:34438336-34438355 None:intergenic 30.0%
AAGTGTGAGAATTTCACCAA+CGG - chr4.4:34438735-34438754 None:intergenic 35.0%
ATAAGGAACATTATCGCTGT+TGG + chr4.4:34439314-34439333 MS.gene28932:intron 35.0%
ATATCATGCAAGGACTTCAA+TGG + chr4.4:34438820-34438839 MS.gene28932:CDS 35.0%
ATTTCGACCCGTTTCATTAA+GGG + chr4.4:34439400-34439419 MS.gene28932:intron 35.0%
GATATAACAGGAAGCGATTT+AGG + chr4.4:34439092-34439111 MS.gene28932:CDS 35.0%
TATACATTGGTGGAAGAGAA+TGG + chr4.4:34439476-34439495 MS.gene28932:intron 35.0%
! AATTGGAAGTGTAACTGAAC+CGG + chr4.4:34439007-34439026 MS.gene28932:CDS 35.0%
! CTTCCAATTTTACCCTCAAT+CGG - chr4.4:34438996-34439015 None:intergenic 35.0%
! GTTTTCTCACTAGAGTAGTT+AGG - chr4.4:34438351-34438370 None:intergenic 35.0%
! TGCGAATCAAGCAATTTTTC+AGG + chr4.4:34438758-34438777 MS.gene28932:CDS 35.0%
!! GTTACCGATTTAGCAGTTAA+TGG - chr4.4:34438657-34438676 None:intergenic 35.0%
!!! TCAAGCAATTTTTCAGGCTT+TGG + chr4.4:34438764-34438783 MS.gene28932:CDS 35.0%
ACCAATGTATATCCTCTCCA+AGG - chr4.4:34439467-34439486 None:intergenic 40.0%
ACCTGTAGATTCTTCTCCTT+CGG - chr4.4:34438442-34438461 None:intergenic 40.0%
AGATTCTTCTCCTTCGGTTT+CGG - chr4.4:34438436-34438455 None:intergenic 40.0%
ATGGCTAAGATCCTGTTCAA+CGG - chr4.4:34439162-34439181 None:intergenic 40.0%
CATCCGATTGAGGGTAAAAT+TGG + chr4.4:34438990-34439009 MS.gene28932:CDS 40.0%
CTACAGGTCTCAAACTTCTA+GGG + chr4.4:34438454-34438473 MS.gene28932:CDS 40.0%
CTCGCCATTAACTGCTAAAT+CGG + chr4.4:34438650-34438669 MS.gene28932:CDS 40.0%
GAAAGATGGTTCATGAACGT+TGG - chr4.4:34438322-34438341 None:intergenic 40.0%
GAAGAGAGTTCAGTTATCTC+CGG - chr4.4:34438543-34438562 None:intergenic 40.0%
GATTTCGACCCGTTTCATTA+AGG + chr4.4:34439399-34439418 MS.gene28932:intron 40.0%
GTTCAGTTATCTCCGGTAAA+AGG - chr4.4:34438536-34438555 None:intergenic 40.0%
TAATGAAACGGGTCGAAATC+CGG - chr4.4:34439399-34439418 None:intergenic 40.0%
TCAACGGTCGTGATCAATTT+CGG - chr4.4:34439146-34439165 None:intergenic 40.0%
TCAATCGGATGAAACTCGAA+CGG - chr4.4:34438981-34439000 None:intergenic 40.0%
TCCTTGGAGAGGATATACAT+TGG + chr4.4:34439463-34439482 MS.gene28932:intron 40.0%
TCTACAGGTCTCAAACTTCT+AGG + chr4.4:34438453-34438472 MS.gene28932:CDS 40.0%
TGAAGTGAAAGTTGAGAGGT+GGG + chr4.4:34439355-34439374 MS.gene28932:intron 40.0%
TTGGAGAGGATATACATTGG+TGG + chr4.4:34439466-34439485 MS.gene28932:intron 40.0%
TTTCGACCCGTTTCATTAAG+GGG + chr4.4:34439401-34439420 MS.gene28932:intron 40.0%
! ATTGGAAGTGTAACTGAACC+GGG + chr4.4:34439008-34439027 MS.gene28932:CDS 40.0%
! CTTTGTTGATTGCTTCTGCT+TGG + chr4.4:34439528-34439547 MS.gene28932:intron 40.0%
! TTCGAGTTTCATCCGATTGA+GGG + chr4.4:34438981-34439000 MS.gene28932:CDS 40.0%
!!! GCAATTTTTCAGGCTTTGGA+TGG + chr4.4:34438768-34438787 MS.gene28932:CDS 40.0%
AAAATCGGCGAGTCTTCTTC+CGG - chr4.4:34438940-34438959 None:intergenic 45.0%
AAGTGAAAGTTGAGAGGTGG+GGG + chr4.4:34439357-34439376 MS.gene28932:intron 45.0%
ACCGAAGGAGAAGAATCTAC+AGG + chr4.4:34438438-34438457 MS.gene28932:CDS 45.0%
ACTGAACTCTCTTCACCGTT+TGG + chr4.4:34438549-34438568 MS.gene28932:CDS 45.0%
AGGAACATTATCGCTGTTGG+TGG + chr4.4:34439317-34439336 MS.gene28932:intron 45.0%
ATGAGGCAATCGTGGTTCAT+TGG + chr4.4:34439051-34439070 MS.gene28932:CDS 45.0%
ATGGTTCTCTCAAGCTTGGT+TGG + chr4.4:34439495-34439514 MS.gene28932:intron 45.0%
ATTGATCACGACCGTTGAAC+AGG + chr4.4:34439148-34439167 MS.gene28932:CDS 45.0%
CGCGTTTCAATCCTACAACT+CGG + chr4.4:34438704-34438723 MS.gene28932:CDS 45.0%
CGGGTTACCCCTTAATGAAA+CGG - chr4.4:34439411-34439430 None:intergenic 45.0%
CGTTTCATTAAGGGGTAACC+CGG + chr4.4:34439409-34439428 MS.gene28932:intron 45.0%
CTACTCTAGTGAGAAAACCG+TGG + chr4.4:34438353-34438372 MS.gene28932:CDS 45.0%
CTGGTGAAGTGAAAGTTGAG+AGG + chr4.4:34439351-34439370 MS.gene28932:intron 45.0%
GAAGTGAAAGTTGAGAGGTG+GGG + chr4.4:34439356-34439375 MS.gene28932:intron 45.0%
GAGAATGGTTCTCTCAAGCT+TGG + chr4.4:34439491-34439510 MS.gene28932:intron 45.0%
GGAACATTATCGCTGTTGGT+GGG + chr4.4:34439318-34439337 MS.gene28932:intron 45.0%
GGGTTACCCCTTAATGAAAC+GGG - chr4.4:34439410-34439429 None:intergenic 45.0%
GTGAAGTGAAAGTTGAGAGG+TGG + chr4.4:34439354-34439373 MS.gene28932:intron 45.0%
TAGCTTCTTAGCGAGGTTTG+TGG + chr4.4:34439187-34439206 MS.gene28932:intron 45.0%
TATCTCCGGTAAAAGGTCGT+TGG - chr4.4:34438529-34438548 None:intergenic 45.0%
TCCGAGCTGTTAGAATCAAC+CGG + chr4.4:34438918-34438937 MS.gene28932:CDS 45.0%
TGAACCACGATTGCCTCATT+CGG - chr4.4:34439050-34439069 None:intergenic 45.0%
TGTTGTTGGGGATGTTTCCT+TGG + chr4.4:34439447-34439466 MS.gene28932:intron 45.0%
TTCACTTCACCAGTCCTCTT+AGG - chr4.4:34439344-34439363 None:intergenic 45.0%
TTCTCTCAAGCTTGGTTGGA+AGG + chr4.4:34439499-34439518 MS.gene28932:intron 45.0%
! GAACGTTGAGATTGCTGACT+CGG + chr4.4:34439114-34439133 MS.gene28932:CDS 45.0%
! GGATAGTGTTGAGAGGCATA+TGG + chr4.4:34439265-34439284 MS.gene28932:intron 45.0%
! GTTCGAGTTTCATCCGATTG+AGG + chr4.4:34438980-34438999 MS.gene28932:CDS 45.0%
! TAGTGTTGAGAGGCATATGG+TGG + chr4.4:34439268-34439287 MS.gene28932:intron 45.0%
! TCCGGTTGATTCTAACAGCT+CGG - chr4.4:34438922-34438941 None:intergenic 45.0%
! TTCACCGTTTGGAACTTCAC+CGG + chr4.4:34438560-34438579 MS.gene28932:CDS 45.0%
!! TCGGTGAGAATTACCGGTTT+CGG + chr4.4:34438888-34438907 MS.gene28932:CDS 45.0%
!! TCTCAGGCGAGTTTGTTGTT+GGG + chr4.4:34439434-34439453 MS.gene28932:intron 45.0%
!! TTCTCAGGCGAGTTTGTTGT+TGG + chr4.4:34439433-34439452 MS.gene28932:intron 45.0%
AAATACGCACCAACTCGCTC+CGG - chr4.4:34438582-34438601 None:intergenic 50.0%
AACAAACTCGCCTGAGAAGC+CGG - chr4.4:34439431-34439450 None:intergenic 50.0%
AACCGGTAATTCTCACCGAG+CGG - chr4.4:34438887-34438906 None:intergenic 50.0%
AGCTGTTGGGGTGTGAGATA+AGG + chr4.4:34439297-34439316 MS.gene28932:intron 50.0%
AGTGAAAGTTGAGAGGTGGG+GGG + chr4.4:34439358-34439377 MS.gene28932:intron 50.0%
ATGCAAGGACTTCAATGGCC+AGG + chr4.4:34438825-34438844 MS.gene28932:CDS 50.0%
ATGCTGGTAGCTTCTTAGCG+AGG + chr4.4:34439180-34439199 MS.gene28932:CDS 50.0%
ATGTGCTGAGTGTGTTGCCA+TGG + chr4.4:34438488-34438507 MS.gene28932:CDS 50.0%
CAACGGTGAAACCGAGTTGT+AGG - chr4.4:34438718-34438737 None:intergenic 50.0%
CGTGTGGTGAGTTCTTGTCT+CGG + chr4.4:34438621-34438640 MS.gene28932:CDS 50.0%
CTACAACTCGGTTTCACCGT+TGG + chr4.4:34438716-34438735 MS.gene28932:CDS 50.0%
CTCGGAAGAAGATCCGAAAC+CGG - chr4.4:34438904-34438923 None:intergenic 50.0%
CTCTAGTGAGAAAACCGTGG+TGG + chr4.4:34438356-34438375 MS.gene28932:CDS 50.0%
CTTCTTAGCGAGGTTTGTGG+AGG + chr4.4:34439190-34439209 MS.gene28932:intron 50.0%
GAAACGGAAACCGAAACCGA+AGG + chr4.4:34438423-34438442 MS.gene28932:CDS 50.0%
GAACAGGATCTTAGCCATGC+TGG + chr4.4:34439164-34439183 MS.gene28932:CDS 50.0%
GCAAGGATGTGGAACAATCC+TGG - chr4.4:34438846-34438865 None:intergenic 50.0%
GTAACTGAACCGGGTCAACT+CGG + chr4.4:34439017-34439036 MS.gene28932:CDS 50.0%
TAGACCGAATGAGGCAATCG+TGG + chr4.4:34439043-34439062 MS.gene28932:CDS 50.0%
TCGCTAAGAAGCTACCAGCA+TGG - chr4.4:34439181-34439200 None:intergenic 50.0%
TCTTCTTCGACCTCGAAGCA+AGG - chr4.4:34438863-34438882 None:intergenic 50.0%
TGTTCCACATCCTTGCTTCG+AGG + chr4.4:34438850-34438869 MS.gene28932:CDS 50.0%
TTGGGGATGTTTCCTTGGAG+AGG + chr4.4:34439452-34439471 MS.gene28932:intron 50.0%
! ATCCGCTCGGTGAGAATTAC+CGG + chr4.4:34438882-34438901 MS.gene28932:CDS 50.0%
! CTTCGCCAACGACCTTTTAC+CGG + chr4.4:34438521-34438540 MS.gene28932:CDS 50.0%
! TGGGTGTGGATAGTGTTGAG+AGG + chr4.4:34439258-34439277 MS.gene28932:intron 50.0%
!! ACTCGGTGTTAGACCGAATG+AGG + chr4.4:34439034-34439053 MS.gene28932:CDS 50.0%
!! CTCAGGCGAGTTTGTTGTTG+GGG + chr4.4:34439435-34439454 MS.gene28932:intron 50.0%
AAAGGTCGTTGGCGAAGTCG+AGG - chr4.4:34438518-34438537 None:intergenic 55.0%
ACAAACTCGCCTGAGAAGCC+GGG - chr4.4:34439430-34439449 None:intergenic 55.0%
ACAAGCGTTACAAGCGCGTG+TGG + chr4.4:34438605-34438624 MS.gene28932:CDS 55.0%
CCCGAGCGAACTCGCAAAAT+CGG - chr4.4:34438955-34438974 None:intergenic 55.0%
CGCTCCGGTGAAGTTCCAAA+CGG - chr4.4:34438567-34438586 None:intergenic 55.0%
GAGGTCGAAGAAGATCCGCT+CGG + chr4.4:34438869-34438888 MS.gene28932:CDS 55.0%
GGCGAAGTCGAGGTTATCCA+TGG - chr4.4:34438508-34438527 None:intergenic 55.0%
TCGACCTCGAAGCAAGGATG+TGG - chr4.4:34438857-34438876 None:intergenic 55.0%
! ATGGTGGAGCAGCAGCTGTT+GGG + chr4.4:34439284-34439303 MS.gene28932:intron 55.0%
! CCGATTTTGCGAGTTCGCTC+GGG + chr4.4:34438952-34438971 MS.gene28932:CDS 55.0%
! GGTCTAACACCGAGTTGACC+CGG - chr4.4:34439029-34439048 None:intergenic 55.0%
! GGTGGGGGGATGAGTTGAAA+CGG + chr4.4:34439372-34439391 MS.gene28932:intron 55.0%
! GTGGGGGGATGAGTTGAAAC+GGG + chr4.4:34439373-34439392 MS.gene28932:intron 55.0%
! TATGGTGGAGCAGCAGCTGT+TGG + chr4.4:34439283-34439302 MS.gene28932:intron 55.0%
! TCTAGGGGATGGTTTGGGTG+TGG + chr4.4:34439244-34439263 MS.gene28932:intron 55.0%
!! GACGCTCTAGGGGATGGTTT+GGG + chr4.4:34439239-34439258 MS.gene28932:intron 55.0%
!! GCCGATTTTGCGAGTTCGCT+CGG + chr4.4:34438951-34438970 MS.gene28932:CDS 55.0%
!! TGAGATTGCTGACTCGGCTG+AGG + chr4.4:34439120-34439139 MS.gene28932:CDS 55.0%
!! TTGTTCGACGCTCTAGGGGA+TGG + chr4.4:34439233-34439252 MS.gene28932:intron 55.0%
AAGGGGTAACCCGGCTTCTC+AGG + chr4.4:34439418-34439437 MS.gene28932:intron 60.0%
AGCGCGTTGTTCGACGCTCT+AGG + chr4.4:34439227-34439246 MS.gene28932:intron 60.0%
CGCGTTGTTCGACGCTCTAG+GGG + chr4.4:34439229-34439248 MS.gene28932:intron 60.0%
CTGTGCGTCGTGTTCCACCA+CGG - chr4.4:34438373-34438392 None:intergenic 60.0%
GCGCGTTGTTCGACGCTCTA+GGG + chr4.4:34439228-34439247 MS.gene28932:intron 60.0%
GGAACTTCACCGGAGCGAGT+TGG + chr4.4:34438570-34438589 MS.gene28932:CDS 60.0%
GTGTTCGCGTTGAGCGCGTT+TGG - chr4.4:34438286-34438305 None:intergenic 60.0%
TGGTGGAGCAGCAGCTGTTG+GGG + chr4.4:34439285-34439304 MS.gene28932:intron 60.0%
! GGGGATGAGTTGAAACGGGC+CGG + chr4.4:34439377-34439396 MS.gene28932:intron 60.0%
! GTTGGTGGGCCTAAGAGGAC+TGG + chr4.4:34439332-34439351 MS.gene28932:intron 60.0%
! TCGCTGTTGGTGGGCCTAAG+AGG + chr4.4:34439327-34439346 MS.gene28932:intron 60.0%
!! CGACGCTCTAGGGGATGGTT+TGG + chr4.4:34439238-34439257 MS.gene28932:intron 60.0%
! CGTGGTGGAACACGACGCAC+AGG + chr4.4:34438371-34438390 MS.gene28932:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 34438276 34439566 34438276 ID=MS.gene28932
chr4.4 mRNA 34438276 34439566 34438276 ID=MS.gene28932.t1;Parent=MS.gene28932
chr4.4 exon 34438276 34439201 34438276 ID=MS.gene28932.t1.exon1;Parent=MS.gene28932.t1
chr4.4 CDS 34438276 34439201 34438276 ID=cds.MS.gene28932.t1;Parent=MS.gene28932.t1
chr4.4 exon 34439554 34439566 34439554 ID=MS.gene28932.t1.exon2;Parent=MS.gene28932.t1
chr4.4 CDS 34439554 34439566 34439554 ID=cds.MS.gene28932.t1;Parent=MS.gene28932.t1
Gene Sequence

>MS.gene28932

ATGAACACCAAACGCGCTCAACGCGAACACGATGACGACGACACCAACGTTCATGAACCATCTTTCAAAAGACCTAACTACTCTAGTGAGAAAACCGTGGTGGAACACGACGCACAGGACGACGAACAACATCAAGTCTTTGAACATGAAACGGAAACCGAAACCGAAGGAGAAGAATCTACAGGTCTCAAACTTCTAGGGCTTTTACTTCAATGTGCTGAGTGTGTTGCCATGGATAACCTCGACTTCGCCAACGACCTTTTACCGGAGATAACTGAACTCTCTTCACCGTTTGGAACTTCACCGGAGCGAGTTGGTGCGTATTTTGCACAAGCGTTACAAGCGCGTGTGGTGAGTTCTTGTCTCGGAAGCTACTCGCCATTAACTGCTAAATCGGTAACGCTAAATCAGTCTCAGAGAATCTTCAACGCGTTTCAATCCTACAACTCGGTTTCACCGTTGGTGAAATTCTCACACTTCACTGCGAATCAAGCAATTTTTCAGGCTTTGGATGGTGAAGATCGTGTCCATATCATTGATCTTGATATCATGCAAGGACTTCAATGGCCAGGATTGTTCCACATCCTTGCTTCGAGGTCGAAGAAGATCCGCTCGGTGAGAATTACCGGTTTCGGATCTTCTTCCGAGCTGTTAGAATCAACCGGAAGAAGACTCGCCGATTTTGCGAGTTCGCTCGGGTTACCGTTCGAGTTTCATCCGATTGAGGGTAAAATTGGAAGTGTAACTGAACCGGGTCAACTCGGTGTTAGACCGAATGAGGCAATCGTGGTTCATTGGATGCATCATTGTTTGTATGATATAACAGGAAGCGATTTAGGAACGTTGAGATTGCTGACTCGGCTGAGGCCGAAATTGATCACGACCGTTGAACAGGATCTTAGCCATGCTGGTAGCTTCTTAGCGAGGTTTGTGGAGGCTTTGCATTATTACAGCGCGTTGTTCGACGCTCTAGGGGATGGTTTGGGTGTGGATAGTGTTGAGAGGCATATGGTGGAGCAGCAGCTGTTGGGGTGTGAGATAAGGAACATTATCGCTGTTGGTGGGCCTAAGAGGACTGGTGAAGTGAAAGTTGAGAGGTGGGGGGATGAGTTGAAACGGGCCGGATTTCGACCCGTTTCATTAAGGGGTAACCCGGCTTCTCAGGCGAGTTTGTTGTTGGGGATGTTTCCTTGGAGAGGATATACATTGGTGGAAGAGAATGGTTCTCTCAAGCTTGGTTGGAAGGATCTCTCTTTGTTGATTGCTTCTGCTTGGCAGCCGTCTGATTTAA
Protein sequence

>MS.gene28932.t1

MNTKRAQREHDDDDTNVHEPSFKRPNYSSEKTVVEHDAQDDEQHQVFEHETETETEGEESTGLKLLGLLLQCAECVAMDNLDFANDLLPEITELSSPFGTSPERVGAYFAQALQARVVSSCLGSYSPLTAKSVTLNQSQRIFNAFQSYNSVSPLVKFSHFTANQAIFQALDGEDRVHIIDLDIMQGLQWPGLFHILASRSKKIRSVRITGFGSSSELLESTGRRLADFASSLGLPFEFHPIEGKIGSVTEPGQLGVRPNEAIVVHWMHHCLYDITGSDLGTLRLLTRLRPKLITTVEQDLSHAGSFLASRLI