AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052305

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052305.t1 MTR_4g076140 96.610 472 16 0 7 478 1 472 0.0 944
MS.gene052305.t1 MTR_5g009080 74.792 480 113 6 6 478 3 481 0.0 734
MS.gene052305.t1 MTR_3g022830 49.888 445 213 4 33 477 4 438 1.30e-161 464
MS.gene052305.t1 MTR_7g027190 34.000 450 215 6 43 475 289 673 2.58e-76 252
MS.gene052305.t1 MTR_5g097480 30.858 431 232 6 51 475 174 544 8.86e-65 218
MS.gene052305.t1 MTR_3g089055 29.837 429 235 6 53 475 202 570 4.67e-60 206
MS.gene052305.t1 MTR_3g089055 29.837 429 235 6 53 475 197 565 4.81e-60 206
MS.gene052305.t1 MTR_1g106590 58.228 158 57 1 8 165 93 241 8.09e-56 189
MS.gene052305.t1 MTR_1g106590 67.033 91 29 1 289 379 251 340 4.10e-24 103
MS.gene052305.t1 MTR_1g029420 29.371 429 237 8 53 475 224 592 9.34e-55 193
MS.gene052305.t1 MTR_2g082090 30.930 430 231 8 51 474 209 578 1.04e-54 192
MS.gene052305.t1 MTR_3g056110 27.477 444 255 8 38 475 160 542 8.16e-51 181
MS.gene052305.t1 MTR_4g104020 28.829 444 234 11 48 475 143 520 1.37e-50 180
MS.gene052305.t1 MTR_4g064160 27.739 429 241 7 54 474 316 683 3.66e-48 176
MS.gene052305.t1 MTR_7g062120 26.869 428 248 5 54 475 372 740 2.06e-46 172
MS.gene052305.t1 MTR_3g022580 40.000 210 85 6 269 478 18 186 8.85e-46 158
MS.gene052305.t1 MTR_4g064120 26.711 453 263 8 27 470 229 621 1.86e-45 168
MS.gene052305.t1 MTR_4g064180 26.490 453 264 8 27 470 229 621 9.93e-44 163
MS.gene052305.t1 MTR_4g064150 26.957 460 262 11 26 474 336 732 9.87e-43 161
MS.gene052305.t1 MTR_2g097410 25.935 428 252 5 54 474 371 740 1.07e-42 161
MS.gene052305.t1 MTR_2g026250 27.336 428 244 10 55 475 229 596 1.39e-42 159
MS.gene052305.t1 MTR_2g097467 25.917 436 237 8 54 474 289 653 1.70e-40 154
MS.gene052305.t1 MTR_2g097473 25.995 427 248 7 54 474 289 653 7.67e-40 152
MS.gene052305.t1 MTR_2g097310 25.499 451 267 9 29 474 251 637 2.68e-39 150
MS.gene052305.t1 MTR_2g097390 25.400 437 261 9 43 474 264 640 1.17e-38 149
MS.gene052305.t1 MTR_2g097350 25.629 437 260 8 43 474 263 639 3.12e-38 147
MS.gene052305.t1 MTR_4g102790 35.349 215 135 1 47 257 285 499 9.29e-38 146
MS.gene052305.t1 MTR_4g102790 32.026 153 97 5 328 474 510 661 7.74e-19 90.1
MS.gene052305.t1 MTR_1g069725 28.146 437 237 12 46 477 101 465 2.76e-37 142
MS.gene052305.t1 MTR_8g442410 43.333 150 83 1 326 475 378 525 1.04e-36 142
MS.gene052305.t1 MTR_8g442410 31.452 248 145 7 47 272 148 392 7.58e-24 104
MS.gene052305.t1 MTR_2g097463 25.761 427 249 7 54 474 290 654 3.28e-36 142
MS.gene052305.t1 MTR_1g096030 28.228 457 230 16 48 476 74 460 5.02e-36 139
MS.gene052305.t1 MTR_2g034280 28.859 447 237 15 45 475 194 575 1.46e-35 139
MS.gene052305.t1 MTR_7g074650 39.267 191 113 2 58 247 442 630 1.65e-35 140
MS.gene052305.t1 MTR_7g074650 37.584 149 88 3 330 475 646 792 9.64e-21 96.3
MS.gene052305.t1 MTR_2g034250 26.829 451 244 12 45 475 201 585 4.01e-34 135
MS.gene052305.t1 MTR_3g065980 33.784 222 133 4 42 258 160 372 8.09e-33 131
MS.gene052305.t1 MTR_3g065980 35.758 165 93 2 324 475 376 540 8.77e-28 116
MS.gene052305.t1 MTR_3g022005 52.041 98 46 1 381 478 32 128 2.33e-31 117
MS.gene052305.t1 MTR_4g097080 24.201 438 251 10 53 475 132 503 3.93e-31 125
MS.gene052305.t1 MTR_2g034260 26.164 451 246 13 45 475 201 584 9.49e-31 125
MS.gene052305.t1 MTR_2g089100 24.115 452 292 10 35 474 30 442 1.24e-30 124
MS.gene052305.t1 MTR_3g072710 25.490 459 251 14 41 474 107 499 1.49e-30 124
MS.gene052305.t1 MTR_7g109580 25.567 485 278 13 7 475 137 554 9.92e-29 119
MS.gene052305.t1 MTR_5g094450 30.667 225 147 3 38 254 146 369 6.35e-28 117
MS.gene052305.t1 MTR_5g094450 38.298 141 86 1 335 475 393 532 5.94e-26 111
MS.gene052305.t1 MTR_4g064200 30.180 222 142 4 54 269 284 498 1.99e-27 116
MS.gene052305.t1 MTR_4g064200 32.000 150 101 1 326 474 500 649 9.90e-17 83.6
MS.gene052305.t1 MTR_4g095500 23.622 508 306 12 3 474 6 467 2.81e-25 108
MS.gene052305.t1 MTR_3g027430 43.220 118 46 1 137 254 184 280 7.26e-25 105
MS.gene052305.t1 MTR_5g058860 30.736 231 143 4 40 253 105 335 2.57e-24 106
MS.gene052305.t1 MTR_5g015490 31.193 218 140 4 48 256 108 324 4.32e-24 105
MS.gene052305.t1 MTR_5g015950 23.918 485 286 14 25 474 9 445 2.27e-23 102
MS.gene052305.t1 MTR_3g053270 23.918 485 286 14 25 474 9 445 2.27e-23 102
MS.gene052305.t1 MTR_2g097380 27.273 209 131 4 64 269 233 423 9.20e-23 101
MS.gene052305.t1 MTR_4g133660 30.233 215 142 3 49 256 170 383 1.32e-22 101
MS.gene052305.t1 MTR_4g133660 34.228 149 97 1 326 474 395 542 1.75e-21 97.8
MS.gene052305.t1 MTR_1g086970 34.911 169 100 5 313 475 314 478 7.21e-22 98.6
MS.gene052305.t1 MTR_1g086970 25.000 248 165 8 45 272 97 343 2.55e-11 66.2
MS.gene052305.t1 MTR_2g099110 30.556 216 138 5 45 250 65 278 1.34e-21 97.4
MS.gene052305.t1 MTR_4g076020 30.570 193 118 4 58 247 88 267 1.88e-21 97.1
MS.gene052305.t1 MTR_4g076020 40.268 149 83 4 329 475 285 429 1.69e-20 94.0
MS.gene052305.t1 MTR_6g047750 26.050 357 192 11 130 475 367 662 1.65e-19 92.0
MS.gene052305.t1 MTR_4g077760 28.251 223 150 3 51 266 190 409 1.75e-19 91.7
MS.gene052305.t1 MTR_4g077760 30.601 183 114 6 301 475 378 555 3.64e-14 75.1
MS.gene052305.t1 MTR_7g057230 31.004 229 131 9 260 474 441 656 8.54e-19 89.7
MS.gene052305.t1 MTR_3g021320 33.987 153 73 2 154 306 1 125 1.07e-18 82.4
MS.gene052305.t1 MTR_0092s0100 33.529 170 105 3 25 194 349 510 2.45e-15 79.3
MS.gene052305.t1 MTR_0092s0100 33.529 170 105 3 25 194 352 513 2.52e-15 79.0
MS.gene052305.t1 MTR_8g093070 29.126 206 132 8 51 251 136 332 3.33e-13 72.0
MS.gene052305.t1 MTR_3g025340 45.902 61 33 0 112 172 9 69 6.80e-12 61.2
MS.gene052305.t1 MTR_4g026485 31.351 185 113 4 55 233 270 446 1.06e-11 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052305.t1 AT1G50420 62.526 483 170 5 5 478 2 482 0.0 613
MS.gene052305.t1 AT1G66350 32.265 437 220 9 42 475 143 506 2.67e-66 222
MS.gene052305.t1 AT2G04890 32.464 422 224 5 54 475 53 413 1.16e-64 215
MS.gene052305.t1 AT5G48150 30.211 427 233 5 55 475 123 490 1.80e-64 216
MS.gene052305.t1 AT5G48150 30.211 427 233 5 55 475 123 490 1.80e-64 216
MS.gene052305.t1 AT5G48150 30.211 427 233 5 55 475 123 490 1.80e-64 216
MS.gene052305.t1 AT5G48150 30.211 427 233 5 55 475 123 490 1.80e-64 216
MS.gene052305.t1 AT5G17490 30.464 453 231 12 37 475 134 516 2.62e-57 198
MS.gene052305.t1 AT1G21450 30.536 429 233 7 53 475 224 593 4.89e-56 196
MS.gene052305.t1 AT1G50600 29.103 457 252 9 30 475 131 526 1.51e-54 191
MS.gene052305.t1 AT1G50600 29.167 456 251 9 31 475 203 597 4.31e-54 191
MS.gene052305.t1 AT1G50600 29.167 456 251 9 31 475 207 601 4.98e-54 191
MS.gene052305.t1 AT1G63100 29.213 445 239 8 46 474 244 628 9.29e-54 191
MS.gene052305.t1 AT1G63100 29.213 445 239 8 46 474 269 653 1.62e-53 191
MS.gene052305.t1 AT1G14920 29.438 445 228 12 42 475 160 529 8.18e-51 181
MS.gene052305.t1 AT5G41920 29.561 433 229 11 51 477 40 402 4.93e-45 162
MS.gene052305.t1 AT1G07530 27.955 440 227 10 54 474 396 764 8.82e-45 167
MS.gene052305.t1 AT2G29060 27.378 431 243 10 54 474 284 654 3.90e-41 156
MS.gene052305.t1 AT2G29060 27.419 434 245 10 51 474 318 691 4.96e-41 156
MS.gene052305.t1 AT1G07520 25.508 443 262 7 43 474 143 528 2.45e-40 152
MS.gene052305.t1 AT1G07520 25.508 443 262 7 43 474 143 528 2.45e-40 152
MS.gene052305.t1 AT1G07520 25.508 443 262 7 43 474 307 692 1.22e-39 152
MS.gene052305.t1 AT1G07520 25.508 443 262 7 43 474 308 693 1.27e-39 152
MS.gene052305.t1 AT5G66770 28.899 436 231 12 51 475 217 584 2.26e-39 150
MS.gene052305.t1 AT3G54220 41.232 211 122 1 39 247 278 488 2.44e-39 151
MS.gene052305.t1 AT3G54220 36.301 146 91 2 332 477 509 652 1.52e-20 95.5
MS.gene052305.t1 AT2G37650 24.826 431 257 6 51 475 344 713 2.83e-37 145
MS.gene052305.t1 AT2G37650 24.826 431 257 6 51 475 332 701 3.18e-37 145
MS.gene052305.t1 AT5G59450 25.806 434 254 7 51 475 224 598 4.90e-37 144
MS.gene052305.t1 AT2G29065 25.571 438 247 10 54 474 252 627 1.78e-36 142
MS.gene052305.t1 AT3G46600 25.694 432 255 6 53 478 3 374 4.17e-35 135
MS.gene052305.t1 AT3G46600 25.694 432 255 6 53 478 179 550 3.68e-34 135
MS.gene052305.t1 AT3G46600 25.618 445 264 7 40 478 199 582 8.53e-34 134
MS.gene052305.t1 AT5G52510 26.411 443 237 13 53 475 267 640 7.79e-31 126
MS.gene052305.t1 AT2G01570 33.480 227 131 5 42 258 212 428 2.93e-30 124
MS.gene052305.t1 AT2G01570 37.086 151 91 3 326 475 434 581 1.37e-26 113
MS.gene052305.t1 AT3G03450 30.942 223 145 5 42 258 171 390 1.57e-28 119
MS.gene052305.t1 AT3G03450 38.854 157 94 2 320 475 390 545 1.71e-28 119
MS.gene052305.t1 AT1G55580 37.584 149 92 1 328 475 297 445 9.49e-28 115
MS.gene052305.t1 AT4G37650 31.579 247 139 7 27 256 131 364 6.63e-24 105
MS.gene052305.t1 AT4G37650 25.871 201 126 7 292 475 335 529 1.34e-11 67.0
MS.gene052305.t1 AT4G17230 34.000 150 98 1 326 475 377 525 6.35e-23 102
MS.gene052305.t1 AT4G17230 28.302 212 145 2 49 254 153 363 5.34e-21 96.3
MS.gene052305.t1 AT4G17230 34.000 150 98 1 326 475 377 525 6.35e-23 102
MS.gene052305.t1 AT4G17230 28.302 212 145 2 49 254 153 363 5.34e-21 96.3
MS.gene052305.t1 AT4G08250 29.588 267 156 10 1 253 68 316 5.02e-20 93.2
MS.gene052305.t1 AT3G50650 31.455 213 127 5 59 263 180 381 2.54e-17 85.1
MS.gene052305.t1 AT3G50650 29.530 149 98 3 334 475 394 542 7.29e-14 74.3
MS.gene052305.t1 AT4G00150 27.041 196 132 6 286 477 368 556 4.60e-12 68.6

Find 107 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACCTCATTGGAGAGATTT+AGG 0.163267 4.4:-34141966 MS.gene052305:CDS
AATGAGCCTCTAAGTTATTT+TGG 0.189632 4.4:-34141820 MS.gene052305:CDS
TTTCATCATCCCAGGCTAAA+AGG 0.243724 4.4:+34142334 None:intergenic
CCGAATGTGCTTCGGTTTAC+TGG 0.250507 4.4:+34142246 None:intergenic
TGCAGCACTATTTGATTGTT+TGG 0.256288 4.4:-34142004 MS.gene052305:CDS
CAATCCTGTTGTCAGCAAAT+TGG 0.264169 4.4:-34142433 MS.gene052305:CDS
CATCATGGAGAAGAGCTTTA+AGG 0.269652 4.4:+34143043 None:intergenic
AGATTTGATTTAGCTGGATT+TGG 0.318965 4.4:-34141844 MS.gene052305:CDS
CAATATCAGCTTGGAGATCT+AGG 0.325494 4.4:-34141690 MS.gene052305:CDS
ATGCCACCTTCATCTGGCTT+TGG 0.334564 4.4:-34143023 MS.gene052305:CDS
AGCATTTGTTCTCACAAATC+AGG 0.349043 4.4:-34142676 MS.gene052305:CDS
TCCATGATGCCACCTTCATC+TGG 0.349847 4.4:-34143029 MS.gene052305:CDS
AACTTACCAAAATAACTTAG+AGG 0.353618 4.4:+34141814 None:intergenic
AATCTCTCCAATGAGGTTCT+TGG 0.353686 4.4:+34141970 None:intergenic
AACCTCATTGGAGAGATTTA+GGG 0.354349 4.4:-34141965 MS.gene052305:CDS
TTGAGTGCTTTGTGGATTCC+AGG 0.357455 4.4:+34142777 None:intergenic
TGCTGACAACAGGATTGAAT+TGG 0.362322 4.4:+34142439 None:intergenic
CAATTTGCTTGAGAAAGATT+TGG 0.366215 4.4:-34142223 MS.gene052305:CDS
ATCTCCACACCCTTGGCTTA+AGG 0.366272 4.4:-34143000 MS.gene052305:CDS
TATAGAATGAAGGAGGAAAA+TGG 0.386592 4.4:-34141751 MS.gene052305:CDS
TGAACTTGAGTCAGGACTTA+TGG 0.391992 4.4:+34142188 None:intergenic
GAGAAACTTCGTGTTAAAAC+CGG 0.400451 4.4:-34142396 MS.gene052305:CDS
CAGGATGAGCACTCAAAACT+TGG 0.403034 4.4:+34142568 None:intergenic
TTACCGAAGCACTTGCTGAT+AGG 0.405714 4.4:-34142815 MS.gene052305:CDS
ATCTCTCCAATGAGGTTCTT+GGG 0.409565 4.4:+34141971 None:intergenic
CACGAAGTTTCTCAAAATCA+AGG 0.414533 4.4:+34142406 None:intergenic
GCTTTGGATCTCCACACCCT+TGG 0.415132 4.4:-34143007 MS.gene052305:CDS
GAGTAGAGTTGAGTGCTTTG+TGG 0.416744 4.4:+34142769 None:intergenic
TTTGAAGTGTTCAAAGAAAC+CGG 0.422703 4.4:+34142159 None:intergenic
TTTATGAGATTTGATTTAGC+TGG 0.436460 4.4:-34141850 MS.gene052305:CDS
AGGCTAAAAGGGTATGCAAT+TGG 0.439239 4.4:+34142346 None:intergenic
AAGAGGTTCATGCAGAGTTA+TGG 0.445441 4.4:-34141784 MS.gene052305:CDS
CGCTGCATAGTATCGCCGTC+AGG 0.446613 4.4:+34142852 None:intergenic
GGAACTGAAATCTGAGGAAA+GGG 0.447352 4.4:-34142979 MS.gene052305:CDS
CCAGTAAACCGAAGCACATT+CGG 0.448222 4.4:-34142246 MS.gene052305:CDS
CTTCATGGTTTCATCATCCC+AGG 0.449792 4.4:+34142326 None:intergenic
GAACAAGACTCTAATCATAA+TGG 0.450476 4.4:-34142069 MS.gene052305:CDS
TGCTAATATGCTGGCAAGAT+AGG 0.451606 4.4:-34141723 MS.gene052305:CDS
TCTGTTACAACCATCACCTT+TGG 0.451615 4.4:+34142090 None:intergenic
AACGATCCCAAGAACCTCAT+TGG 0.456113 4.4:-34141977 MS.gene052305:CDS
AAAGGAAAGGCATGAAAAGC+TGG 0.456379 4.4:-34141881 MS.gene052305:CDS
GCAAATTGCCGAATGTGCTT+CGG 0.471544 4.4:+34142238 None:intergenic
TTCATCATCCCAGGCTAAAA+GGG 0.472082 4.4:+34142335 None:intergenic
CAAGATTACAAGCAGCTACA+TGG 0.487046 4.4:+34142919 None:intergenic
CAACTCTACTCGAGTGTCAA+TGG 0.491983 4.4:-34142757 MS.gene052305:CDS
TTGCACAAGTGAGCAACAAA+TGG 0.493036 4.4:+34142943 None:intergenic
ATGTGCTTCGGTTTACTGGA+TGG 0.504499 4.4:+34142250 None:intergenic
CTCAAATGTGGAGAGCCATC+AGG 0.504592 4.4:+34142549 None:intergenic
ACAAATGCTACTTTCATGAA+AGG 0.509715 4.4:+34142690 None:intergenic
AGGATACTTAAAGCATGGCC+TGG 0.511478 4.4:-34142795 MS.gene052305:CDS
TTGTAAGTGAATTCCATTCG+AGG 0.515092 4.4:+34142278 None:intergenic
CAAATGCTACTTTCATGAAA+GGG 0.516070 4.4:+34142691 None:intergenic
TCAGGCTATCATTGAAGCAA+TGG 0.520201 4.4:-34142658 MS.gene052305:CDS
GCCAGATGAAGGTGGCATCA+TGG 0.521665 4.4:+34143028 None:intergenic
TCCATGCTTGCTTCCCCTGA+CGG 0.525920 4.4:-34142867 MS.gene052305:CDS
AAGAATATTATTGCATGTGA+GGG 0.527409 4.4:-34141913 MS.gene052305:CDS
CAAGAATATTATTGCATGTG+AGG 0.529680 4.4:-34141914 MS.gene052305:CDS
GCCGTCAGGGGAAGCAAGCA+TGG 0.529802 4.4:+34142866 None:intergenic
TCGAATGGAATTCACTTACA+AGG 0.530422 4.4:-34142276 MS.gene052305:CDS
AGAAACTTCGTGTTAAAACC+GGG 0.531168 4.4:-34142395 MS.gene052305:CDS
GGCATGAAAAGCTGGATAAG+TGG 0.531394 4.4:-34141873 MS.gene052305:CDS
GGTGATGATGAACTTGAGTC+AGG 0.534611 4.4:+34142180 None:intergenic
ATGTGAGGGATTAGAAAGAA+AGG 0.534942 4.4:-34141899 MS.gene052305:CDS
TATCCTATCAGCAAGTGCTT+CGG 0.537933 4.4:+34142812 None:intergenic
ACTAATTGCTAGTGCCTCCC+CGG 0.543403 4.4:+34142377 None:intergenic
TGACTCAAGTTCATCATCAC+CGG 0.544363 4.4:-34142178 MS.gene052305:CDS
AGGAACTGAAATCTGAGGAA+AGG 0.544931 4.4:-34142980 MS.gene052305:CDS
GATTTCAGTTCCTTAAGCCA+AGG 0.546043 4.4:+34142990 None:intergenic
TTGTGAGGCATATAGAATGA+AGG 0.546355 4.4:-34141761 MS.gene052305:CDS
GTACAGCTATGTTGCAAGAA+GGG 0.546911 4.4:-34143088 MS.gene052305:CDS
AGAAATGAAATCCACTGAGC+AGG 0.550035 4.4:+34142591 None:intergenic
CCAAGCTGATATTGAAAACA+AGG 0.551538 4.4:+34141699 None:intergenic
AGTTCCTTAAGCCAAGGGTG+TGG 0.551766 4.4:+34142996 None:intergenic
ATGGCTGTGTGCTAATATGC+TGG 0.553571 4.4:-34141732 MS.gene052305:CDS
CAATCAGTCTATGAGCTACC+TGG 0.553683 4.4:+34142490 None:intergenic
GAAGAAAGAGGTTCTCGACC+AGG 0.556759 4.4:-34142508 MS.gene052305:CDS
ATTTCAGTTCCTTAAGCCAA+GGG 0.557071 4.4:+34142991 None:intergenic
CTCTTTCTTCTGATGAACGC+CGG 0.562200 4.4:+34142521 None:intergenic
AGTGTAGAGCTTCTAGTAGC+CGG 0.564547 4.4:+34142031 None:intergenic
TCTCCACATTTGAGAATCAC+CGG 0.570075 4.4:-34142540 MS.gene052305:CDS
GGTACAGCTATGTTGCAAGA+AGG 0.572547 4.4:-34143089 MS.gene052305:CDS
GGAGATCCAAAGCCAGATGA+AGG 0.572774 4.4:+34143017 None:intergenic
CTCATTGGAGAGATTTAGGG+TGG 0.573947 4.4:-34141962 MS.gene052305:CDS
GAGTTTATCACCAAAGGTGA+TGG 0.577759 4.4:-34142100 MS.gene052305:CDS
ACAACAGGATTGAATTGGAA+TGG 0.580239 4.4:+34142444 None:intergenic
CATGCAGAGTTATGGTTGTG+AGG 0.584714 4.4:-34141776 MS.gene052305:CDS
GCTGCATAGTATCGCCGTCA+GGG 0.585579 4.4:+34142853 None:intergenic
GCTATCATTGAAGCAATGGA+AGG 0.591127 4.4:-34142654 MS.gene052305:CDS
CGGCGTTCATCAGAAGAAAG+AGG 0.593327 4.4:-34142520 MS.gene052305:CDS
TATCATTGAAGCAATGGAAG+GGG 0.594602 4.4:-34142652 MS.gene052305:CDS
CTATCATTGAAGCAATGGAA+GGG 0.603065 4.4:-34142653 MS.gene052305:CDS
TTGGTAAGTTGCAAGCAAAG+AGG 0.603635 4.4:-34141801 MS.gene052305:CDS
CTGATAGGATACTTAAAGCA+TGG 0.603739 4.4:-34142800 MS.gene052305:CDS
AGACATTTCTCAATGCATTG+TGG 0.605127 4.4:-34142122 MS.gene052305:CDS
GCTTAAGGAACTGAAATCTG+AGG 0.605648 4.4:-34142985 MS.gene052305:CDS
ATTGTGGAGTTTATCACCAA+AGG 0.618172 4.4:-34142106 MS.gene052305:CDS
TGAGGCATATAGAATGAAGG+AGG 0.622680 4.4:-34141758 MS.gene052305:CDS
AGGGATTAGAAAGAAAGGAA+AGG 0.622681 4.4:-34141894 MS.gene052305:CDS
ACGCCGGTGATTCTCAAATG+TGG 0.623446 4.4:+34142537 None:intergenic
CTGCATAGTATCGCCGTCAG+GGG 0.626726 4.4:+34142854 None:intergenic
CACCCTAAATCTCTCCAATG+AGG 0.633605 4.4:+34141963 None:intergenic
ATGCTGCTGAACCTGCTCAG+TGG 0.654432 4.4:-34142602 MS.gene052305:CDS
GATCCAAAGCCAGATGAAGG+TGG 0.658186 4.4:+34143020 None:intergenic
GAAACTTCGTGTTAAAACCG+GGG 0.688408 4.4:-34142394 MS.gene052305:CDS
GAACTGAAATCTGAGGAAAG+GGG 0.705778 4.4:-34142978 MS.gene052305:CDS
ACTTCGTGTTAAAACCGGGG+AGG 0.735326 4.4:-34142391 MS.gene052305:CDS
CTGACGGCGATACTATGCAG+CGG 0.754112 4.4:-34142851 MS.gene052305:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AACTTACCAAAATAACTTAG+AGG + chr4.4:34142971-34142990 None:intergenic 25.0%
! AAGAATATTATTGCATGTGA+GGG - chr4.4:34142869-34142888 MS.gene052305:CDS 25.0%
!! TTTATGAGATTTGATTTAGC+TGG - chr4.4:34142932-34142951 MS.gene052305:CDS 25.0%
CAAGAATATTATTGCATGTG+AGG - chr4.4:34142868-34142887 MS.gene052305:CDS 30.0%
GAACAAGACTCTAATCATAA+TGG - chr4.4:34142713-34142732 MS.gene052305:CDS 30.0%
TATAGAATGAAGGAGGAAAA+TGG - chr4.4:34143031-34143050 MS.gene052305:CDS 30.0%
! AATGAGCCTCTAAGTTATTT+TGG - chr4.4:34142962-34142981 MS.gene052305:CDS 30.0%
! AGATTTGATTTAGCTGGATT+TGG - chr4.4:34142938-34142957 MS.gene052305:CDS 30.0%
! CAATTTGCTTGAGAAAGATT+TGG - chr4.4:34142559-34142578 MS.gene052305:CDS 30.0%
! CTTTTGTTAAAAACCTCGAA+TGG - chr4.4:34142491-34142510 MS.gene052305:CDS 30.0%
! TCATAATGGCTCACATTTTA+CGG - chr4.4:34142727-34142746 MS.gene052305:CDS 30.0%
! TTCGAGGTTTTTAACAAAAG+AGG + chr4.4:34142491-34142510 None:intergenic 30.0%
! TTTGAAGTGTTCAAAGAAAC+CGG + chr4.4:34142626-34142645 None:intergenic 30.0%
!! ACAAATGCTACTTTCATGAA+AGG + chr4.4:34142095-34142114 None:intergenic 30.0%
!! CAAATGCTACTTTCATGAAA+GGG + chr4.4:34142094-34142113 None:intergenic 30.0%
!!! AAGAGGAGATTTTTTCTTCA+TGG + chr4.4:34142474-34142493 None:intergenic 30.0%
AACCTCATTGGAGAGATTTA+GGG - chr4.4:34142817-34142836 MS.gene052305:CDS 35.0%
ACAACAGGATTGAATTGGAA+TGG + chr4.4:34142341-34142360 None:intergenic 35.0%
AGAAACTTCGTGTTAAAACC+GGG - chr4.4:34142387-34142406 MS.gene052305:CDS 35.0%
AGACATTTCTCAATGCATTG+TGG - chr4.4:34142660-34142679 MS.gene052305:CDS 35.0%
AGCATTTGTTCTCACAAATC+AGG - chr4.4:34142106-34142125 MS.gene052305:CDS 35.0%
AGGGATTAGAAAGAAAGGAA+AGG - chr4.4:34142888-34142907 MS.gene052305:CDS 35.0%
ATGTGAGGGATTAGAAAGAA+AGG - chr4.4:34142883-34142902 MS.gene052305:CDS 35.0%
ATTGTGGAGTTTATCACCAA+AGG - chr4.4:34142676-34142695 MS.gene052305:CDS 35.0%
ATTTCAGTTCCTTAAGCCAA+GGG + chr4.4:34141794-34141813 None:intergenic 35.0%
CACGAAGTTTCTCAAAATCA+AGG + chr4.4:34142379-34142398 None:intergenic 35.0%
CCAAGCTGATATTGAAAACA+AGG + chr4.4:34143086-34143105 None:intergenic 35.0%
CTATCATTGAAGCAATGGAA+GGG - chr4.4:34142129-34142148 MS.gene052305:CDS 35.0%
CTGATAGGATACTTAAAGCA+TGG - chr4.4:34141982-34142001 MS.gene052305:CDS 35.0%
GAGAAACTTCGTGTTAAAAC+CGG - chr4.4:34142386-34142405 MS.gene052305:CDS 35.0%
TATCATTGAAGCAATGGAAG+GGG - chr4.4:34142130-34142149 MS.gene052305:CDS 35.0%
TCGAATGGAATTCACTTACA+AGG - chr4.4:34142506-34142525 MS.gene052305:CDS 35.0%
TGCAGCACTATTTGATTGTT+TGG - chr4.4:34142778-34142797 MS.gene052305:CDS 35.0%
TTGTAAGTGAATTCCATTCG+AGG + chr4.4:34142507-34142526 None:intergenic 35.0%
TTGTGAGGCATATAGAATGA+AGG - chr4.4:34143021-34143040 MS.gene052305:CDS 35.0%
!!! CCTTGTTTTCAATATCAGCT+TGG - chr4.4:34143083-34143102 MS.gene052305:CDS 35.0%
!!! TTTTCCAATTTGCTGACAAC+AGG + chr4.4:34142356-34142375 None:intergenic 35.0%
AAGAGGTTCATGCAGAGTTA+TGG - chr4.4:34142998-34143017 MS.gene052305:CDS 40.0%
AATCTCTCCAATGAGGTTCT+TGG + chr4.4:34142815-34142834 None:intergenic 40.0%
AGAAATGAAATCCACTGAGC+AGG + chr4.4:34142194-34142213 None:intergenic 40.0%
AGGAACTGAAATCTGAGGAA+AGG - chr4.4:34141802-34141821 MS.gene052305:CDS 40.0%
ATCTCTCCAATGAGGTTCTT+GGG + chr4.4:34142814-34142833 None:intergenic 40.0%
CAAGATTACAAGCAGCTACA+TGG + chr4.4:34141866-34141885 None:intergenic 40.0%
CAATATCAGCTTGGAGATCT+AGG - chr4.4:34143092-34143111 MS.gene052305:CDS 40.0%
CAATCCTGTTGTCAGCAAAT+TGG - chr4.4:34142349-34142368 MS.gene052305:CDS 40.0%
CATCATGGAGAAGAGCTTTA+AGG + chr4.4:34141742-34141761 None:intergenic 40.0%
GAAACTTCGTGTTAAAACCG+GGG - chr4.4:34142388-34142407 MS.gene052305:CDS 40.0%
GAACCTCATTGGAGAGATTT+AGG - chr4.4:34142816-34142835 MS.gene052305:CDS 40.0%
GAACTGAAATCTGAGGAAAG+GGG - chr4.4:34141804-34141823 MS.gene052305:CDS 40.0%
GAGTTTATCACCAAAGGTGA+TGG - chr4.4:34142682-34142701 MS.gene052305:CDS 40.0%
GATTTCAGTTCCTTAAGCCA+AGG + chr4.4:34141795-34141814 None:intergenic 40.0%
GCTATCATTGAAGCAATGGA+AGG - chr4.4:34142128-34142147 MS.gene052305:CDS 40.0%
GCTTAAGGAACTGAAATCTG+AGG - chr4.4:34141797-34141816 MS.gene052305:CDS 40.0%
GGAACTGAAATCTGAGGAAA+GGG - chr4.4:34141803-34141822 MS.gene052305:CDS 40.0%
GTACAGCTATGTTGCAAGAA+GGG - chr4.4:34141694-34141713 MS.gene052305:CDS 40.0%
TATCCTATCAGCAAGTGCTT+CGG + chr4.4:34141973-34141992 None:intergenic 40.0%
TCAGGCTATCATTGAAGCAA+TGG - chr4.4:34142124-34142143 MS.gene052305:CDS 40.0%
TCTCCACATTTGAGAATCAC+CGG - chr4.4:34142242-34142261 MS.gene052305:CDS 40.0%
TCTGTTACAACCATCACCTT+TGG + chr4.4:34142695-34142714 None:intergenic 40.0%
TGAACTTGAGTCAGGACTTA+TGG + chr4.4:34142597-34142616 None:intergenic 40.0%
TGACTCAAGTTCATCATCAC+CGG - chr4.4:34142604-34142623 MS.gene052305:CDS 40.0%
TGAGGCATATAGAATGAAGG+AGG - chr4.4:34143024-34143043 MS.gene052305:CDS 40.0%
TGCTAATATGCTGGCAAGAT+AGG - chr4.4:34143059-34143078 MS.gene052305:CDS 40.0%
TGCTGACAACAGGATTGAAT+TGG + chr4.4:34142346-34142365 None:intergenic 40.0%
TTCATCATCCCAGGCTAAAA+GGG + chr4.4:34142450-34142469 None:intergenic 40.0%
TTGCACAAGTGAGCAACAAA+TGG + chr4.4:34141842-34141861 None:intergenic 40.0%
TTGGTAAGTTGCAAGCAAAG+AGG - chr4.4:34142981-34143000 MS.gene052305:CDS 40.0%
TTTCATCATCCCAGGCTAAA+AGG + chr4.4:34142451-34142470 None:intergenic 40.0%
! AAAGGAAAGGCATGAAAAGC+TGG - chr4.4:34142901-34142920 MS.gene052305:CDS 40.0%
! AATTGCATACCCTTTTAGCC+TGG - chr4.4:34142438-34142457 MS.gene052305:CDS 40.0%
! AGGCTAAAAGGGTATGCAAT+TGG + chr4.4:34142439-34142458 None:intergenic 40.0%
! AGGGTGGAGAAACTTCTTTT+CGG - chr4.4:34142836-34142855 MS.gene052305:CDS 40.0%
! ATTGCATACCCTTTTAGCCT+GGG - chr4.4:34142439-34142458 MS.gene052305:CDS 40.0%
AACGATCCCAAGAACCTCAT+TGG - chr4.4:34142805-34142824 MS.gene052305:CDS 45.0%
AGGATACTTAAAGCATGGCC+TGG - chr4.4:34141987-34142006 MS.gene052305:CDS 45.0%
AGTGTAGAGCTTCTAGTAGC+CGG + chr4.4:34142754-34142773 None:intergenic 45.0%
ATGGCTGTGTGCTAATATGC+TGG - chr4.4:34143050-34143069 MS.gene052305:CDS 45.0%
ATGTGCTTCGGTTTACTGGA+TGG + chr4.4:34142535-34142554 None:intergenic 45.0%
CAACTCTACTCGAGTGTCAA+TGG - chr4.4:34142025-34142044 MS.gene052305:CDS 45.0%
CAATCAGTCTATGAGCTACC+TGG + chr4.4:34142295-34142314 None:intergenic 45.0%
CACCCTAAATCTCTCCAATG+AGG + chr4.4:34142822-34142841 None:intergenic 45.0%
CAGGATGAGCACTCAAAACT+TGG + chr4.4:34142217-34142236 None:intergenic 45.0%
CATGCAGAGTTATGGTTGTG+AGG - chr4.4:34143006-34143025 MS.gene052305:CDS 45.0%
CCAGTAAACCGAAGCACATT+CGG - chr4.4:34142536-34142555 MS.gene052305:CDS 45.0%
CTCATTGGAGAGATTTAGGG+TGG - chr4.4:34142820-34142839 MS.gene052305:CDS 45.0%
CTCTTTCTTCTGATGAACGC+CGG + chr4.4:34142264-34142283 None:intergenic 45.0%
CTTCATGGTTTCATCATCCC+AGG + chr4.4:34142459-34142478 None:intergenic 45.0%
GAGTAGAGTTGAGTGCTTTG+TGG + chr4.4:34142016-34142035 None:intergenic 45.0%
GCAAATTGCCGAATGTGCTT+CGG + chr4.4:34142547-34142566 None:intergenic 45.0%
GGCATGAAAAGCTGGATAAG+TGG - chr4.4:34142909-34142928 MS.gene052305:CDS 45.0%
GGTACAGCTATGTTGCAAGA+AGG - chr4.4:34141693-34141712 MS.gene052305:CDS 45.0%
TTGAGTGCTTTGTGGATTCC+AGG + chr4.4:34142008-34142027 None:intergenic 45.0%
! ATGGCTCACATTTTACGGAC+CGG - chr4.4:34142732-34142751 MS.gene052305:CDS 45.0%
! GGAGAAACTTCTTTTCGGTG+AGG - chr4.4:34142841-34142860 MS.gene052305:CDS 45.0%
! GGTGATGATGAACTTGAGTC+AGG + chr4.4:34142605-34142624 None:intergenic 45.0%
! TTACCGAAGCACTTGCTGAT+AGG - chr4.4:34141967-34141986 MS.gene052305:CDS 45.0%
!! GTTTTGAGTGCTCATCCTGA+TGG - chr4.4:34142218-34142237 MS.gene052305:CDS 45.0%
ACGCCGGTGATTCTCAAATG+TGG + chr4.4:34142248-34142267 None:intergenic 50.0%
ACTAATTGCTAGTGCCTCCC+CGG + chr4.4:34142408-34142427 None:intergenic 50.0%
ACTTCGTGTTAAAACCGGGG+AGG - chr4.4:34142391-34142410 MS.gene052305:CDS 50.0%
AGTTCCTTAAGCCAAGGGTG+TGG + chr4.4:34141789-34141808 None:intergenic 50.0%
ATCTCCACACCCTTGGCTTA+AGG - chr4.4:34141782-34141801 MS.gene052305:CDS 50.0%
CCGAATGTGCTTCGGTTTAC+TGG + chr4.4:34142539-34142558 None:intergenic 50.0%
CGGCGTTCATCAGAAGAAAG+AGG - chr4.4:34142262-34142281 MS.gene052305:CDS 50.0%
CTCAAATGTGGAGAGCCATC+AGG + chr4.4:34142236-34142255 None:intergenic 50.0%
GAAGAAAGAGGTTCTCGACC+AGG - chr4.4:34142274-34142293 MS.gene052305:CDS 50.0%
GATCCAAAGCCAGATGAAGG+TGG + chr4.4:34141765-34141784 None:intergenic 50.0%
GGAGATCCAAAGCCAGATGA+AGG + chr4.4:34141768-34141787 None:intergenic 50.0%
TCCATGATGCCACCTTCATC+TGG - chr4.4:34141753-34141772 MS.gene052305:CDS 50.0%
! ATGCCACCTTCATCTGGCTT+TGG - chr4.4:34141759-34141778 MS.gene052305:CDS 50.0%
ATGCTGCTGAACCTGCTCAG+TGG - chr4.4:34142180-34142199 MS.gene052305:CDS 55.0%
CTGACGGCGATACTATGCAG+CGG - chr4.4:34141931-34141950 MS.gene052305:CDS 55.0%
CTGCATAGTATCGCCGTCAG+GGG + chr4.4:34141931-34141950 None:intergenic 55.0%
GCCAGATGAAGGTGGCATCA+TGG + chr4.4:34141757-34141776 None:intergenic 55.0%
GCTGCATAGTATCGCCGTCA+GGG + chr4.4:34141932-34141951 None:intergenic 55.0%
TCCATGCTTGCTTCCCCTGA+CGG - chr4.4:34141915-34141934 MS.gene052305:CDS 55.0%
! GCTTTGGATCTCCACACCCT+TGG - chr4.4:34141775-34141794 MS.gene052305:CDS 55.0%
CGCTGCATAGTATCGCCGTC+AGG + chr4.4:34141933-34141952 None:intergenic 60.0%
GCCGTCAGGGGAAGCAAGCA+TGG + chr4.4:34141919-34141938 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 34141684 34143120 34141684 ID=MS.gene052305
chr4.4 mRNA 34141684 34143120 34141684 ID=MS.gene052305.t1;Parent=MS.gene052305
chr4.4 exon 34141684 34143120 34141684 ID=MS.gene052305.t1.exon1;Parent=MS.gene052305.t1
chr4.4 CDS 34141684 34143120 34141684 ID=cds.MS.gene052305.t1;Parent=MS.gene052305.t1
Gene Sequence

>MS.gene052305

ATGTTACATGGTACAGCTATGTTGCAAGAAGGGTCATCTTCTGTTACTTCTTCAGCCTTAAAGCTCTTCTCCATGATGCCACCTTCATCTGGCTTTGGATCTCCACACCCTTGGCTTAAGGAACTGAAATCTGAGGAAAGGGGTTTGTACTTGATCCATTTGTTGCTCACTTGTGCAAACCATGTAGCTGCTTGTAATCTTGAGCATGCAAATGCAACACTTGAGCAAATTTCCATGCTTGCTTCCCCTGACGGCGATACTATGCAGCGGATTGCAGCGTATTTTACCGAAGCACTTGCTGATAGGATACTTAAAGCATGGCCTGGAATCCACAAAGCACTCAACTCTACTCGAGTGTCAATGGTTTCAGAAAAGATTCTTGTGCAGAAGCTCTTTTTTGAGCTTTTCCCTTTCATGAAAGTAGCATTTGTTCTCACAAATCAGGCTATCATTGAAGCAATGGAAGGGGAGAGAATGATTCACATAATTGATCTCAATGCTGCTGAACCTGCTCAGTGGATTTCATTTCTCCAAGTTTTGAGTGCTCATCCTGATGGCTCTCCACATTTGAGAATCACCGGCGTTCATCAGAAGAAAGAGGTTCTCGACCAGGTAGCTCATAGACTGATTGCAGAAGCTGAAAAATTAGATATACCATTCCAATTCAATCCTGTTGTCAGCAAATTGGAAAACCTTGATTTTGAGAAACTTCGTGTTAAAACCGGGGAGGCACTAGCAATTAGTTCAATTCTCCAATTGCATACCCTTTTAGCCTGGGATGATGAAACCATGAAGAAAAAATCTCCTCTTTTGTTAAAAACCTCGAATGGAATTCACTTACAAGGATTCCATCCAGTAAACCGAAGCACATTCGGCAATTTGCTTGAGAAAGATTTGGTTAGCAGCTATACCATAAGTCCTGACTCAAGTTCATCATCACCGGTTTCTTTGAACACTTCAAATTCAATGAACACAGAGACATTTCTCAATGCATTGTGGAGTTTATCACCAAAGGTGATGGTTGTAACAGAACAAGACTCTAATCATAATGGCTCACATTTTACGGACCGGCTACTAGAAGCTCTACACTCTTATGCAGCACTATTTGATTGTTTGGAATCAACGATCCCAAGAACCTCATTGGAGAGATTTAGGGTGGAGAAACTTCTTTTCGGTGAGGAAATCAAGAATATTATTGCATGTGAGGGATTAGAAAGAAAGGAAAGGCATGAAAAGCTGGATAAGTGGTTTATGAGATTTGATTTAGCTGGATTTGGTAATGAGCCTCTAAGTTATTTTGGTAAGTTGCAAGCAAAGAGGTTCATGCAGAGTTATGGTTGTGAGGCATATAGAATGAAGGAGGAAAATGGCTGTGTGCTAATATGCTGGCAAGATAGGTCCTTGTTTTCAATATCAGCTTGGAGATCTAGGAAGTAA
Protein sequence

>MS.gene052305.t1

MLHGTAMLQEGSSSVTSSALKLFSMMPPSSGFGSPHPWLKELKSEERGLYLIHLLLTCANHVAACNLEHANATLEQISMLASPDGDTMQRIAAYFTEALADRILKAWPGIHKALNSTRVSMVSEKILVQKLFFELFPFMKVAFVLTNQAIIEAMEGERMIHIIDLNAAEPAQWISFLQVLSAHPDGSPHLRITGVHQKKEVLDQVAHRLIAEAEKLDIPFQFNPVVSKLENLDFEKLRVKTGEALAISSILQLHTLLAWDDETMKKKSPLLLKTSNGIHLQGFHPVNRSTFGNLLEKDLVSSYTISPDSSSSSPVSLNTSNSMNTETFLNALWSLSPKVMVVTEQDSNHNGSHFTDRLLEALHSYAALFDCLESTIPRTSLERFRVEKLLFGEEIKNIIACEGLERKERHEKLDKWFMRFDLAGFGNEPLSYFGKLQAKRFMQSYGCEAYRMKEENGCVLICWQDRSLFSISAWRSRK