AlfalfaGEDB | MS.gene28926

M. sativa cultivar XinJiangDaYe / MS.gene28926

KEGG: -
GO: -
PFAM: PF03514.17

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28926.t1	XP_003607301.1	96	472	19	0	1	472	1	472	1.10E-258	902.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28926.t1	Q9LPR8	62.8	481	168	5	1	472	4	482	9.7e-168	591.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28926.t1	G7JQJ6	96.0	472	19	0	1	472	1	472	7.7e-259	902.1

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene28926.t1	TF	GRAS

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052305	MS.gene28926	0.868009	8.97E-66	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28926.t1	MTR_4g076140	95.975	472	19	0	1	472	1	472	0.0	915
MS.gene28926.t1	MTR_5g009080	74.739	479	113	6	1	472	4	481	0.0	730
MS.gene28926.t1	MTR_3g022830	49.888	445	213	4	27	471	4	438	9.24e-161	462
MS.gene28926.t1	MTR_7g027190	33.778	450	216	6	37	469	289	673	4.22e-74	246
MS.gene28926.t1	MTR_5g097480	31.323	431	230	6	45	469	174	544	3.42e-66	222
MS.gene28926.t1	MTR_1g029420	29.837	429	235	8	47	469	224	592	8.76e-56	195
MS.gene28926.t1	MTR_1g106590	58.228	158	57	1	2	159	93	241	8.97e-56	189
MS.gene28926.t1	MTR_1g106590	69.318	88	26	1	285	372	253	339	2.27e-23	101
MS.gene28926.t1	MTR_3g089055	29.138	429	238	8	47	469	202	570	5.72e-54	190
MS.gene28926.t1	MTR_3g089055	29.138	429	238	8	47	469	197	565	7.52e-54	189

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28926.t1	AT1G50420	62.786	481	168	5	1	472	4	482	0.0	609
MS.gene28926.t1	AT1G66350	32.037	437	221	8	36	469	143	506	6.85e-68	226
MS.gene28926.t1	AT5G48150	30.679	427	231	5	49	469	123	490	4.99e-66	220
MS.gene28926.t1	AT5G48150	30.679	427	231	5	49	469	123	490	4.99e-66	220
MS.gene28926.t1	AT5G48150	30.679	427	231	5	49	469	123	490	4.99e-66	220
MS.gene28926.t1	AT5G48150	30.679	427	231	5	49	469	123	490	4.99e-66	220
MS.gene28926.t1	AT2G04890	32.938	422	222	5	48	469	53	413	2.36e-65	216
MS.gene28926.t1	AT5G17490	30.905	453	229	12	31	469	134	516	1.40e-59	204
MS.gene28926.t1	AT1G50600	29.322	457	251	10	24	469	131	526	1.63e-58	201
MS.gene28926.t1	AT1G50600	29.386	456	250	10	25	469	207	601	4.21e-58	202

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Find 109 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTCATTCGAGAGATTTA+GGG	0.358753	4.4:-34330088	MS.gene28926:CDS
AAAGGAAAGGCATGAAAAGC+TGG	0.456379	4.4:-34330004	MS.gene28926:CDS
AACTTACCAAAATAACTTAG+AGG	0.353618	4.4:+34329937	None:intergenic
AAGAACATTATTGCATGTGA+GGG	0.567712	4.4:-34330036	MS.gene28926:CDS
AAGAGGTTCATGCAGAGTTA+TGG	0.445441	4.4:-34329907	MS.gene28926:CDS
AATCTCTCGAATGAGTTTCT+TGG	0.330855	4.4:+34330093	None:intergenic
AATGAGCCTCTAAGTTATTT+TGG	0.189632	4.4:-34329943	MS.gene28926:CDS
ACAAATGCTACTTTCATGAA+AGG	0.509715	4.4:+34330813	None:intergenic
ACAACAGGATTGAATTGGAA+TGG	0.580239	4.4:+34330567	None:intergenic
ACGCCGGTGATTCTCAAATG+TGG	0.623446	4.4:+34330660	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACTCATTCGAGAGATTTA+GGG	-	chr4.4:34330922-34330941	MS.gene28926:CDS	30.0%
AAGAACATTATTGCATGTGA+GGG	-	chr4.4:34330974-34330993	MS.gene28926:CDS	30.0%
CTATTATTGAAGCAATGGAA+GGG	-	chr4.4:34330234-34330253	MS.gene28926:CDS	30.0%
GAACAAGACTCTAATCATAA+TGG	-	chr4.4:34330818-34330837	MS.gene28926:CDS	30.0%
TATAGAATGAAGGAGGAAAA+TGG	-	chr4.4:34331136-34331155	MS.gene28926:CDS	30.0%
TATTATTGAAGCAATGGAAG+GGG	-	chr4.4:34330235-34330254	MS.gene28926:CDS	30.0%
AATCTCTCGAATGAGTTTCT+TGG	+	chr4.4:34330920-34330939	None:intergenic	35.0%
ACAACAGGATTGAATTGGAA+TGG	+	chr4.4:34330446-34330465	None:intergenic	35.0%
AGAAACTTCGTGTTAAAACC+GGG	-	chr4.4:34330492-34330511	MS.gene28926:CDS	35.0%
AGACATTTCTCAATGCATTG+TGG	-	chr4.4:34330765-34330784	MS.gene28926:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	34329807	34331225	34329807	ID=MS.gene28926
chr4.4	mRNA	34329807	34331225	34329807	ID=MS.gene28926.t1;Parent=MS.gene28926
chr4.4	exon	34329807	34331225	34329807	ID=MS.gene28926.t1.exon1;Parent=MS.gene28926.t1
chr4.4	CDS	34329807	34331225	34329807	ID=cds.MS.gene28926.t1;Parent=MS.gene28926.t1

Gene Sequence

>MS.gene28926

ATGTTGCAAGAAGGGTCATCTTCTGTTACTTCTTCAGCCTTAAAGCTCTTCTCCATGATGCCACCTTCATCTGGCTTTGGATCTCCACACCCTTGGCTTAAGGAACTGAAATCTGAGGAAAGGGGTTTGTACTTGATCCATTTGTTGCTCACTTGTGCAAACCATGTAGCTGCTTGTAATCTTGAGCATGCAAATGCAACACTTGAGCAAATTTCCATGCTTGCTTCCCCTGACGGCGATACTATGCAGCGGATTGCAGCGTATTTTACCGAAGCACTTGCTGATAGGATACTTAAAGCATGGCCTGGAATCCACAAAGCACTCAACTCTACTCGAGTGTCGATGGTTTCAGAAAAGATTCTTGTGCAGAAGCTCTTTTTTGAGCTTTTCCCTTTCATGAAAGTAGCATTTGTTCTCACAAATCAGGCTATTATTGAAGCAATGGAAGGGGAGAGAATGATTCACATAATTGATCTCAATGCTGCTGAACCTGCTCAGTGGATTTCACTTCTCCAAGTTTTGAGTGCTCATCCTGATGGCCCTCCACATTTGAGAATCACCGGCGTTCATCAGAAGAAAGAGGTTCTCGACCAGGTAGCTCATAGACTGATTGCAGAAGCTGAAAAATTAGATATACCATTCCAATTCAATCCTGTTGTCAGCAAATTGGAAAACCTTGATTTTGAGAAACTTCGTGTTAAAACCGGGGAGGCACTAGCAATTAGTTCAATTCTCCAATTGCATACCCTTTTAGCCTGGGATGATGAAACCATGAAGAAAAAATCTCCTCTTTTGTTAAAAACCTCGAATGGAATTCACTTACAAGGATTCCATCCAGTAAACCGAAGCATATTCGGCAATTTGCTTGAGAAAGATTTGGTTAGCAGCTATACCATAAGTCCTGACTCAAGTTCATCATCACCGGTTTCTTTGAACACTTCAAATTCAATGAACACAGAGACATTTCTCAATGCATTGTGGAGTTTATCACCAAAGGTGATGGTTGTAACAGAACAAGACTCTAATCATAATGGCTCACATTTTACGGACCGGCTACTAGAAGCTCTACACTCTTATGCAGCACTATTTGATTGTTTGGAATCAACGGTCCCAAGAAACTCATTCGAGAGATTTAGGGTGGAGAAACTTCATTTTGGTGAGGAAATCAAGAACATTATTGCATGTGAGGGATTAGAAAGAAAGGAAAGGCATGAAAAGCTGGATAAGTGGTTTATGAGATTTGATTTAGCTGGATTTGGTAATGAGCCTCTAAGTTATTTTGGTAAGTTGCAAGCAAAGAGGTTCATGCAGAGTTATGGTTGTGAGGCATATAGAATGAAGGAGGAAAATGGCTGTGTGCTAATATGCTGGCAAGATAGGTCCTTGTTTTCAATATCAGCTTGGAGATCTAGGAAGTAA

Protein sequence

>MS.gene28926.t1

MLQEGSSSVTSSALKLFSMMPPSSGFGSPHPWLKELKSEERGLYLIHLLLTCANHVAACNLEHANATLEQISMLASPDGDTMQRIAAYFTEALADRILKAWPGIHKALNSTRVSMVSEKILVQKLFFELFPFMKVAFVLTNQAIIEAMEGERMIHIIDLNAAEPAQWISLLQVLSAHPDGPPHLRITGVHQKKEVLDQVAHRLIAEAEKLDIPFQFNPVVSKLENLDFEKLRVKTGEALAISSILQLHTLLAWDDETMKKKSPLLLKTSNGIHLQGFHPVNRSIFGNLLEKDLVSSYTISPDSSSSSPVSLNTSNSMNTETFLNALWSLSPKVMVVTEQDSNHNGSHFTDRLLEALHSYAALFDCLESTVPRNSFERFRVEKLHFGEEIKNIIACEGLERKERHEKLDKWFMRFDLAGFGNEPLSYFGKLQAKRFMQSYGCEAYRMKEENGCVLICWQDRSLFSISAWRSRK