AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28926

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28926.t1 MTR_4g076140 95.975 472 19 0 1 472 1 472 0.0 915
MS.gene28926.t1 MTR_5g009080 74.739 479 113 6 1 472 4 481 0.0 730
MS.gene28926.t1 MTR_3g022830 49.888 445 213 4 27 471 4 438 9.24e-161 462
MS.gene28926.t1 MTR_7g027190 33.778 450 216 6 37 469 289 673 4.22e-74 246
MS.gene28926.t1 MTR_5g097480 31.323 431 230 6 45 469 174 544 3.42e-66 222
MS.gene28926.t1 MTR_1g029420 29.837 429 235 8 47 469 224 592 8.76e-56 195
MS.gene28926.t1 MTR_1g106590 58.228 158 57 1 2 159 93 241 8.97e-56 189
MS.gene28926.t1 MTR_1g106590 69.318 88 26 1 285 372 253 339 2.27e-23 101
MS.gene28926.t1 MTR_3g089055 29.138 429 238 8 47 469 202 570 5.72e-54 190
MS.gene28926.t1 MTR_3g089055 29.138 429 238 8 47 469 197 565 7.52e-54 189
MS.gene28926.t1 MTR_3g056110 27.928 444 253 8 32 469 160 542 1.90e-52 185
MS.gene28926.t1 MTR_8g442410 29.955 444 230 12 41 469 148 525 4.27e-51 182
MS.gene28926.t1 MTR_4g064160 28.438 429 238 7 48 468 316 683 8.85e-50 181
MS.gene28926.t1 MTR_4g104020 28.798 441 238 11 42 469 143 520 7.29e-49 175
MS.gene28926.t1 MTR_4g064120 26.490 453 264 7 21 464 229 621 5.33e-47 172
MS.gene28926.t1 MTR_7g062120 27.103 428 247 5 48 469 372 740 1.89e-46 172
MS.gene28926.t1 MTR_4g064180 26.269 453 265 7 21 464 229 621 2.68e-45 167
MS.gene28926.t1 MTR_2g026250 27.804 428 242 10 49 469 229 596 2.43e-44 164
MS.gene28926.t1 MTR_4g064150 27.174 460 261 11 20 468 336 732 4.43e-44 165
MS.gene28926.t1 MTR_2g097410 26.168 428 251 5 48 468 371 740 7.29e-44 164
MS.gene28926.t1 MTR_3g022580 39.048 210 87 6 263 472 18 186 3.35e-43 151
MS.gene28926.t1 MTR_2g097467 26.376 436 235 8 48 468 289 653 3.02e-41 156
MS.gene28926.t1 MTR_2g097473 26.464 427 246 7 48 468 289 653 2.75e-40 154
MS.gene28926.t1 MTR_2g097350 27.002 437 254 9 37 468 263 639 1.11e-39 152
MS.gene28926.t1 MTR_2g097390 25.172 437 262 8 37 468 264 640 2.18e-38 148
MS.gene28926.t1 MTR_4g102790 35.349 215 135 1 41 251 285 499 3.70e-38 147
MS.gene28926.t1 MTR_4g102790 31.373 153 98 5 322 468 510 661 2.79e-18 88.2
MS.gene28926.t1 MTR_2g034280 29.306 447 235 15 39 469 194 575 5.58e-38 146
MS.gene28926.t1 MTR_1g096030 27.948 458 230 14 42 470 74 460 1.11e-37 143
MS.gene28926.t1 MTR_7g074650 40.314 191 111 2 52 241 442 630 3.22e-37 145
MS.gene28926.t1 MTR_7g074650 36.913 149 89 3 324 469 646 792 5.88e-20 93.6
MS.gene28926.t1 MTR_1g069725 27.689 437 239 12 40 471 101 465 4.64e-37 142
MS.gene28926.t1 MTR_2g097463 26.279 430 249 7 45 468 287 654 7.04e-37 144
MS.gene28926.t1 MTR_2g034250 27.938 451 239 14 39 469 201 585 1.43e-36 142
MS.gene28926.t1 MTR_3g065980 34.685 222 131 4 36 252 160 372 1.98e-34 135
MS.gene28926.t1 MTR_3g065980 35.758 165 93 2 318 469 376 540 3.47e-28 117
MS.gene28926.t1 MTR_2g034260 26.608 451 244 13 39 469 201 584 3.66e-33 132
MS.gene28926.t1 MTR_4g097080 24.429 438 250 10 47 469 132 503 1.14e-31 127
MS.gene28926.t1 MTR_2g089100 24.115 452 292 10 29 468 30 442 2.75e-31 125
MS.gene28926.t1 MTR_3g072710 25.708 459 250 14 35 468 107 499 1.13e-30 124
MS.gene28926.t1 MTR_2g082090 33.778 225 139 3 45 263 209 429 1.48e-30 125
MS.gene28926.t1 MTR_2g082090 41.611 149 86 1 320 468 431 578 2.90e-29 121
MS.gene28926.t1 MTR_3g022005 51.546 97 46 1 376 472 33 128 3.32e-30 114
MS.gene28926.t1 MTR_5g094450 31.556 225 145 3 32 248 146 369 9.36e-30 122
MS.gene28926.t1 MTR_5g094450 38.298 141 86 1 329 469 393 532 1.75e-25 109
MS.gene28926.t1 MTR_2g097310 28.352 261 171 5 23 275 251 503 1.84e-29 122
MS.gene28926.t1 MTR_4g064200 31.111 225 142 4 45 263 281 498 4.51e-29 120
MS.gene28926.t1 MTR_4g064200 32.000 150 101 1 320 468 500 649 5.13e-16 81.3
MS.gene28926.t1 MTR_7g109580 25.567 485 278 13 1 469 137 554 1.06e-28 119
MS.gene28926.t1 MTR_3g027430 44.915 118 44 1 131 248 184 280 1.56e-26 110
MS.gene28926.t1 MTR_4g095500 24.187 492 293 11 11 468 22 467 2.55e-25 108
MS.gene28926.t1 MTR_5g015950 24.330 485 284 14 19 468 9 445 4.52e-25 107
MS.gene28926.t1 MTR_3g053270 24.330 485 284 14 19 468 9 445 4.52e-25 107
MS.gene28926.t1 MTR_5g058860 31.169 231 142 4 34 247 105 335 9.66e-25 107
MS.gene28926.t1 MTR_5g058860 26.667 150 107 3 321 468 348 496 5.45e-11 65.1
MS.gene28926.t1 MTR_5g015490 31.651 218 139 4 42 250 108 324 1.80e-24 106
MS.gene28926.t1 MTR_4g133660 31.163 215 140 3 43 250 170 383 2.80e-24 106
MS.gene28926.t1 MTR_4g133660 34.228 149 97 1 320 468 395 542 1.90e-21 97.8
MS.gene28926.t1 MTR_2g097380 27.273 209 131 4 58 263 233 423 2.91e-23 103
MS.gene28926.t1 MTR_2g099110 31.019 216 137 5 39 244 65 278 7.43e-22 98.2
MS.gene28926.t1 MTR_1g086970 33.728 169 102 5 307 469 314 478 2.11e-21 97.1
MS.gene28926.t1 MTR_1g086970 25.000 248 165 8 39 266 97 343 9.17e-12 67.4
MS.gene28926.t1 MTR_4g076020 30.052 193 119 4 52 241 88 267 1.33e-20 94.4
MS.gene28926.t1 MTR_4g076020 39.597 149 84 4 323 469 285 429 1.36e-19 91.3
MS.gene28926.t1 MTR_3g021320 35.294 153 71 2 148 300 1 125 5.45e-20 86.3
MS.gene28926.t1 MTR_4g077760 28.700 223 149 3 45 260 190 409 8.97e-20 92.4
MS.gene28926.t1 MTR_4g077760 30.055 183 115 6 295 469 378 555 1.89e-13 72.8
MS.gene28926.t1 MTR_6g047750 26.331 357 191 11 124 469 367 662 1.91e-19 91.7
MS.gene28926.t1 MTR_4g026485 25.059 427 242 11 49 469 270 624 3.09e-19 91.3
MS.gene28926.t1 MTR_7g057230 33.333 150 95 3 320 468 511 656 3.71e-19 90.9
MS.gene28926.t1 MTR_0092s0100 32.941 170 106 3 19 188 349 510 2.10e-14 76.3
MS.gene28926.t1 MTR_0092s0100 32.941 170 106 3 19 188 352 513 2.10e-14 76.3
MS.gene28926.t1 MTR_8g093070 29.126 206 132 8 45 245 136 332 2.50e-13 72.4
MS.gene28926.t1 MTR_3g025340 45.902 61 33 0 106 166 9 69 7.48e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28926.t1 AT1G50420 62.786 481 168 5 1 472 4 482 0.0 609
MS.gene28926.t1 AT1G66350 32.037 437 221 8 36 469 143 506 6.85e-68 226
MS.gene28926.t1 AT5G48150 30.679 427 231 5 49 469 123 490 4.99e-66 220
MS.gene28926.t1 AT5G48150 30.679 427 231 5 49 469 123 490 4.99e-66 220
MS.gene28926.t1 AT5G48150 30.679 427 231 5 49 469 123 490 4.99e-66 220
MS.gene28926.t1 AT5G48150 30.679 427 231 5 49 469 123 490 4.99e-66 220
MS.gene28926.t1 AT2G04890 32.938 422 222 5 48 469 53 413 2.36e-65 216
MS.gene28926.t1 AT5G17490 30.905 453 229 12 31 469 134 516 1.40e-59 204
MS.gene28926.t1 AT1G50600 29.322 457 251 10 24 469 131 526 1.63e-58 201
MS.gene28926.t1 AT1G50600 29.386 456 250 10 25 469 207 601 4.21e-58 202
MS.gene28926.t1 AT1G50600 29.386 456 250 10 25 469 203 597 4.63e-58 202
MS.gene28926.t1 AT1G21450 30.769 429 232 7 47 469 224 593 4.84e-56 196
MS.gene28926.t1 AT1G14920 30.112 445 225 12 36 469 160 529 4.88e-53 187
MS.gene28926.t1 AT2G01570 29.888 445 226 13 36 469 212 581 1.22e-51 184
MS.gene28926.t1 AT5G41920 30.023 433 227 11 45 471 40 402 7.07e-46 164
MS.gene28926.t1 AT1G07530 27.955 440 227 10 48 468 396 764 5.33e-44 165
MS.gene28926.t1 AT3G54220 42.180 211 120 1 33 241 278 488 6.11e-41 155
MS.gene28926.t1 AT3G54220 35.616 146 92 2 326 471 509 652 7.31e-20 93.2
MS.gene28926.t1 AT2G29060 26.267 434 250 8 45 468 318 691 5.05e-40 153
MS.gene28926.t1 AT2G29060 27.189 434 246 10 45 468 281 654 8.22e-40 152
MS.gene28926.t1 AT1G07520 25.282 443 263 7 37 468 143 528 3.62e-39 149
MS.gene28926.t1 AT1G07520 25.282 443 263 7 37 468 143 528 3.62e-39 149
MS.gene28926.t1 AT5G66770 28.899 436 231 12 45 469 217 584 7.56e-39 149
MS.gene28926.t1 AT1G07520 25.282 443 263 7 37 468 307 692 1.94e-38 149
MS.gene28926.t1 AT1G07520 25.282 443 263 7 37 468 308 693 2.05e-38 149
MS.gene28926.t1 AT5G59450 25.806 434 254 7 45 469 224 598 1.19e-37 145
MS.gene28926.t1 AT2G29065 25.571 438 247 10 48 468 252 627 9.58e-37 143
MS.gene28926.t1 AT3G46600 25.926 432 254 6 47 472 3 374 2.31e-36 138
MS.gene28926.t1 AT2G37650 24.826 431 257 6 45 469 344 713 4.72e-36 142
MS.gene28926.t1 AT2G37650 24.826 431 257 6 45 469 332 701 5.64e-36 141
MS.gene28926.t1 AT3G46600 25.926 432 254 6 47 472 179 550 6.40e-35 137
MS.gene28926.t1 AT3G46600 25.926 432 254 6 47 472 211 582 1.24e-34 137
MS.gene28926.t1 AT1G63100 32.218 239 157 4 40 274 269 506 1.89e-31 128
MS.gene28926.t1 AT1G63100 33.125 160 94 2 322 468 494 653 1.34e-20 95.5
MS.gene28926.t1 AT1G63100 32.218 239 157 4 40 274 244 481 2.38e-31 127
MS.gene28926.t1 AT1G63100 33.125 160 94 2 322 468 469 628 9.29e-21 95.9
MS.gene28926.t1 AT5G52510 26.376 436 246 13 47 469 267 640 2.65e-30 124
MS.gene28926.t1 AT3G03450 31.839 223 143 5 36 252 171 390 2.79e-30 124
MS.gene28926.t1 AT3G03450 38.854 157 94 2 314 469 390 545 9.82e-29 119
MS.gene28926.t1 AT1G55580 38.255 149 91 1 322 469 297 445 3.92e-28 116
MS.gene28926.t1 AT1G55580 27.843 255 143 8 44 258 40 293 4.87e-13 71.2
MS.gene28926.t1 AT4G37650 31.984 247 138 7 21 250 131 364 3.31e-24 105
MS.gene28926.t1 AT3G50650 26.218 431 236 12 53 469 180 542 5.63e-24 105
MS.gene28926.t1 AT4G17230 34.000 150 98 1 320 469 377 525 5.91e-23 102
MS.gene28926.t1 AT4G17230 29.245 212 143 2 43 248 153 363 1.31e-22 101
MS.gene28926.t1 AT4G17230 34.000 150 98 1 320 469 377 525 5.91e-23 102
MS.gene28926.t1 AT4G17230 29.245 212 143 2 43 248 153 363 1.31e-22 101
MS.gene28926.t1 AT4G08250 30.116 259 153 9 3 247 72 316 2.75e-21 97.1
MS.gene28926.t1 AT3G49950 22.126 461 276 14 26 468 12 407 6.44e-16 80.1
MS.gene28926.t1 AT4G00150 27.179 195 133 5 280 471 368 556 6.82e-12 68.2

Find 109 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACTCATTCGAGAGATTT+AGG 0.125804 4.4:-34330089 MS.gene28926:CDS
CCGAATATGCTTCGGTTTAC+TGG 0.158109 4.4:+34330369 None:intergenic
AATGAGCCTCTAAGTTATTT+TGG 0.189632 4.4:-34329943 MS.gene28926:CDS
TTTCATCATCCCAGGCTAAA+AGG 0.243724 4.4:+34330457 None:intergenic
TGCAGCACTATTTGATTGTT+TGG 0.256288 4.4:-34330127 MS.gene28926:CDS
CAATCCTGTTGTCAGCAAAT+TGG 0.264169 4.4:-34330556 MS.gene28926:CDS
CATCATGGAGAAGAGCTTTA+AGG 0.269652 4.4:+34331166 None:intergenic
AGATTTGATTTAGCTGGATT+TGG 0.318965 4.4:-34329967 MS.gene28926:CDS
CAATATCAGCTTGGAGATCT+AGG 0.325494 4.4:-34329813 MS.gene28926:CDS
AATCTCTCGAATGAGTTTCT+TGG 0.330855 4.4:+34330093 None:intergenic
ATGCCACCTTCATCTGGCTT+TGG 0.334564 4.4:-34331146 MS.gene28926:CDS
CCAGTAAACCGAAGCATATT+CGG 0.339215 4.4:-34330369 MS.gene28926:CDS
AGCATTTGTTCTCACAAATC+AGG 0.349043 4.4:-34330799 MS.gene28926:CDS
TCCATGATGCCACCTTCATC+TGG 0.349847 4.4:-34331152 MS.gene28926:CDS
AACTTACCAAAATAACTTAG+AGG 0.353618 4.4:+34329937 None:intergenic
TTGAGTGCTTTGTGGATTCC+AGG 0.357455 4.4:+34330900 None:intergenic
AAACTCATTCGAGAGATTTA+GGG 0.358753 4.4:-34330088 MS.gene28926:CDS
TGCTGACAACAGGATTGAAT+TGG 0.362322 4.4:+34330562 None:intergenic
CAATTTGCTTGAGAAAGATT+TGG 0.366215 4.4:-34330346 MS.gene28926:CDS
ATCTCCACACCCTTGGCTTA+AGG 0.366272 4.4:-34331123 MS.gene28926:CDS
TATAGAATGAAGGAGGAAAA+TGG 0.386592 4.4:-34329874 MS.gene28926:CDS
TGAACTTGAGTCAGGACTTA+TGG 0.391992 4.4:+34330311 None:intergenic
ATCTCTCGAATGAGTTTCTT+GGG 0.394420 4.4:+34330094 None:intergenic
CTCAAATGTGGAGGGCCATC+AGG 0.400333 4.4:+34330672 None:intergenic
GAGAAACTTCGTGTTAAAAC+CGG 0.400451 4.4:-34330519 MS.gene28926:CDS
CAGGATGAGCACTCAAAACT+TGG 0.403034 4.4:+34330691 None:intergenic
TTACCGAAGCACTTGCTGAT+AGG 0.405714 4.4:-34330938 MS.gene28926:CDS
CACGAAGTTTCTCAAAATCA+AGG 0.414533 4.4:+34330529 None:intergenic
GCTTTGGATCTCCACACCCT+TGG 0.415132 4.4:-34331130 MS.gene28926:CDS
GAGTAGAGTTGAGTGCTTTG+TGG 0.416744 4.4:+34330892 None:intergenic
TTTGAAGTGTTCAAAGAAAC+CGG 0.422703 4.4:+34330282 None:intergenic
TTTATGAGATTTGATTTAGC+TGG 0.436460 4.4:-34329973 MS.gene28926:CDS
AGGCTAAAAGGGTATGCAAT+TGG 0.439239 4.4:+34330469 None:intergenic
AGGGTGGAGAAACTTCATTT+TGG 0.439547 4.4:-34330069 MS.gene28926:CDS
AAGAGGTTCATGCAGAGTTA+TGG 0.445441 4.4:-34329907 MS.gene28926:CDS
CGCTGCATAGTATCGCCGTC+AGG 0.446613 4.4:+34330975 None:intergenic
GGAACTGAAATCTGAGGAAA+GGG 0.447352 4.4:-34331102 MS.gene28926:CDS
CTTCATGGTTTCATCATCCC+AGG 0.449792 4.4:+34330449 None:intergenic
GAACAAGACTCTAATCATAA+TGG 0.450476 4.4:-34330192 MS.gene28926:CDS
TGCTAATATGCTGGCAAGAT+AGG 0.451606 4.4:-34329846 MS.gene28926:CDS
TCTGTTACAACCATCACCTT+TGG 0.451615 4.4:+34330213 None:intergenic
AAAGGAAAGGCATGAAAAGC+TGG 0.456379 4.4:-34330004 MS.gene28926:CDS
TTCATCATCCCAGGCTAAAA+GGG 0.472082 4.4:+34330458 None:intergenic
GCAAATTGCCGAATATGCTT+CGG 0.476281 4.4:+34330361 None:intergenic
CAAGATTACAAGCAGCTACA+TGG 0.487046 4.4:+34331042 None:intergenic
TTGCACAAGTGAGCAACAAA+TGG 0.493036 4.4:+34331066 None:intergenic
ATATGCTTCGGTTTACTGGA+TGG 0.504223 4.4:+34330373 None:intergenic
ACAAATGCTACTTTCATGAA+AGG 0.509715 4.4:+34330813 None:intergenic
AGGATACTTAAAGCATGGCC+TGG 0.511478 4.4:-34330918 MS.gene28926:CDS
TTGTAAGTGAATTCCATTCG+AGG 0.515092 4.4:+34330401 None:intergenic
CAAATGCTACTTTCATGAAA+GGG 0.516070 4.4:+34330814 None:intergenic
TCAGGCTATTATTGAAGCAA+TGG 0.520201 4.4:-34330781 MS.gene28926:CDS
GCCAGATGAAGGTGGCATCA+TGG 0.521665 4.4:+34331151 None:intergenic
TCCATGCTTGCTTCCCCTGA+CGG 0.525920 4.4:-34330990 MS.gene28926:CDS
CAAGAACATTATTGCATGTG+AGG 0.529680 4.4:-34330037 MS.gene28926:CDS
GCCGTCAGGGGAAGCAAGCA+TGG 0.529802 4.4:+34330989 None:intergenic
TCGAATGGAATTCACTTACA+AGG 0.530422 4.4:-34330399 MS.gene28926:CDS
AGAAACTTCGTGTTAAAACC+GGG 0.531168 4.4:-34330518 MS.gene28926:CDS
GGCATGAAAAGCTGGATAAG+TGG 0.531394 4.4:-34329996 MS.gene28926:CDS
GGTGATGATGAACTTGAGTC+AGG 0.534611 4.4:+34330303 None:intergenic
ATGTGAGGGATTAGAAAGAA+AGG 0.534942 4.4:-34330022 MS.gene28926:CDS
TATCCTATCAGCAAGTGCTT+CGG 0.537933 4.4:+34330935 None:intergenic
ATTTGATTGTTTGGAATCAA+CGG 0.538609 4.4:-34330118 MS.gene28926:CDS
ACTAATTGCTAGTGCCTCCC+CGG 0.543403 4.4:+34330500 None:intergenic
TGACTCAAGTTCATCATCAC+CGG 0.544363 4.4:-34330301 MS.gene28926:CDS
AGGAACTGAAATCTGAGGAA+AGG 0.544931 4.4:-34331103 MS.gene28926:CDS
GATTTCAGTTCCTTAAGCCA+AGG 0.546043 4.4:+34331113 None:intergenic
TTGTGAGGCATATAGAATGA+AGG 0.546355 4.4:-34329884 MS.gene28926:CDS
GTACAGCTATGTTGCAAGAA+GGG 0.546911 4.4:-34331211 None:intergenic
CCAAGCTGATATTGAAAACA+AGG 0.551538 4.4:+34329822 None:intergenic
AGTTCCTTAAGCCAAGGGTG+TGG 0.551766 4.4:+34331119 None:intergenic
AGAAGTGAAATCCACTGAGC+AGG 0.552331 4.4:+34330714 None:intergenic
ATGGCTGTGTGCTAATATGC+TGG 0.553571 4.4:-34329855 MS.gene28926:CDS
CAATCAGTCTATGAGCTACC+TGG 0.553683 4.4:+34330613 None:intergenic
CCTCCACATTTGAGAATCAC+CGG 0.556070 4.4:-34330663 MS.gene28926:CDS
GAAGAAAGAGGTTCTCGACC+AGG 0.556759 4.4:-34330631 MS.gene28926:CDS
ATTTCAGTTCCTTAAGCCAA+GGG 0.557071 4.4:+34331114 None:intergenic
CTCTTTCTTCTGATGAACGC+CGG 0.562200 4.4:+34330644 None:intergenic
AGTGTAGAGCTTCTAGTAGC+CGG 0.564547 4.4:+34330154 None:intergenic
GCTATTATTGAAGCAATGGA+AGG 0.565201 4.4:-34330777 MS.gene28926:CDS
CTCATTCGAGAGATTTAGGG+TGG 0.565611 4.4:-34330085 MS.gene28926:CDS
AAGAACATTATTGCATGTGA+GGG 0.567712 4.4:-34330036 MS.gene28926:CDS
GGTACAGCTATGTTGCAAGA+AGG 0.572547 4.4:-34331212 None:intergenic
GGAGATCCAAAGCCAGATGA+AGG 0.572774 4.4:+34331140 None:intergenic
GAGTTTATCACCAAAGGTGA+TGG 0.577759 4.4:-34330223 MS.gene28926:CDS
ACAACAGGATTGAATTGGAA+TGG 0.580239 4.4:+34330567 None:intergenic
CATGCAGAGTTATGGTTGTG+AGG 0.584714 4.4:-34329899 MS.gene28926:CDS
GCTGCATAGTATCGCCGTCA+GGG 0.585579 4.4:+34330976 None:intergenic
CAACTCTACTCGAGTGTCGA+TGG 0.592320 4.4:-34330880 MS.gene28926:CDS
CGGCGTTCATCAGAAGAAAG+AGG 0.593327 4.4:-34330643 MS.gene28926:CDS
TATTATTGAAGCAATGGAAG+GGG 0.594602 4.4:-34330775 MS.gene28926:CDS
TTGGTAAGTTGCAAGCAAAG+AGG 0.603635 4.4:-34329924 MS.gene28926:CDS
CTGATAGGATACTTAAAGCA+TGG 0.603739 4.4:-34330923 MS.gene28926:CDS
AGACATTTCTCAATGCATTG+TGG 0.605127 4.4:-34330245 MS.gene28926:CDS
GCTTAAGGAACTGAAATCTG+AGG 0.605648 4.4:-34331108 MS.gene28926:CDS
CTATTATTGAAGCAATGGAA+GGG 0.615371 4.4:-34330776 MS.gene28926:CDS
ATTGTGGAGTTTATCACCAA+AGG 0.618172 4.4:-34330229 MS.gene28926:CDS
CGGTGATTCTCAAATGTGGA+GGG 0.619559 4.4:+34330664 None:intergenic
TGAGGCATATAGAATGAAGG+AGG 0.622680 4.4:-34329881 MS.gene28926:CDS
AGGGATTAGAAAGAAAGGAA+AGG 0.622681 4.4:-34330017 MS.gene28926:CDS
ACGCCGGTGATTCTCAAATG+TGG 0.623446 4.4:+34330660 None:intergenic
CTGCATAGTATCGCCGTCAG+GGG 0.626726 4.4:+34330977 None:intergenic
CCGGTGATTCTCAAATGTGG+AGG 0.626889 4.4:+34330663 None:intergenic
ATGCTGCTGAACCTGCTCAG+TGG 0.654432 4.4:-34330725 MS.gene28926:CDS
GATCCAAAGCCAGATGAAGG+TGG 0.658186 4.4:+34331143 None:intergenic
GAAACTTCGTGTTAAAACCG+GGG 0.688408 4.4:-34330517 MS.gene28926:CDS
GAACTGAAATCTGAGGAAAG+GGG 0.705778 4.4:-34331101 MS.gene28926:CDS
ACTTCGTGTTAAAACCGGGG+AGG 0.735326 4.4:-34330514 MS.gene28926:CDS
CTGACGGCGATACTATGCAG+CGG 0.754112 4.4:-34330974 MS.gene28926:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AACTTACCAAAATAACTTAG+AGG + chr4.4:34331076-34331095 None:intergenic 25.0%
!! ATTTGATTGTTTGGAATCAA+CGG - chr4.4:34330892-34330911 MS.gene28926:CDS 25.0%
!! TTTATGAGATTTGATTTAGC+TGG - chr4.4:34331037-34331056 MS.gene28926:CDS 25.0%
AAACTCATTCGAGAGATTTA+GGG - chr4.4:34330922-34330941 MS.gene28926:CDS 30.0%
AAGAACATTATTGCATGTGA+GGG - chr4.4:34330974-34330993 MS.gene28926:CDS 30.0%
CTATTATTGAAGCAATGGAA+GGG - chr4.4:34330234-34330253 MS.gene28926:CDS 30.0%
GAACAAGACTCTAATCATAA+TGG - chr4.4:34330818-34330837 MS.gene28926:CDS 30.0%
TATAGAATGAAGGAGGAAAA+TGG - chr4.4:34331136-34331155 MS.gene28926:CDS 30.0%
TATTATTGAAGCAATGGAAG+GGG - chr4.4:34330235-34330254 MS.gene28926:CDS 30.0%
! AATGAGCCTCTAAGTTATTT+TGG - chr4.4:34331067-34331086 MS.gene28926:CDS 30.0%
! AGATTTGATTTAGCTGGATT+TGG - chr4.4:34331043-34331062 MS.gene28926:CDS 30.0%
! CAATTTGCTTGAGAAAGATT+TGG - chr4.4:34330664-34330683 MS.gene28926:CDS 30.0%
! CTTTTGTTAAAAACCTCGAA+TGG - chr4.4:34330596-34330615 MS.gene28926:CDS 30.0%
! TCATAATGGCTCACATTTTA+CGG - chr4.4:34330832-34330851 MS.gene28926:CDS 30.0%
! TTCGAGGTTTTTAACAAAAG+AGG + chr4.4:34330596-34330615 None:intergenic 30.0%
! TTTGAAGTGTTCAAAGAAAC+CGG + chr4.4:34330731-34330750 None:intergenic 30.0%
!! ACAAATGCTACTTTCATGAA+AGG + chr4.4:34330200-34330219 None:intergenic 30.0%
!! CAAATGCTACTTTCATGAAA+GGG + chr4.4:34330199-34330218 None:intergenic 30.0%
!!! AAGAGGAGATTTTTTCTTCA+TGG + chr4.4:34330579-34330598 None:intergenic 30.0%
AATCTCTCGAATGAGTTTCT+TGG + chr4.4:34330920-34330939 None:intergenic 35.0%
ACAACAGGATTGAATTGGAA+TGG + chr4.4:34330446-34330465 None:intergenic 35.0%
AGAAACTTCGTGTTAAAACC+GGG - chr4.4:34330492-34330511 MS.gene28926:CDS 35.0%
AGACATTTCTCAATGCATTG+TGG - chr4.4:34330765-34330784 MS.gene28926:CDS 35.0%
AGCATTTGTTCTCACAAATC+AGG - chr4.4:34330211-34330230 MS.gene28926:CDS 35.0%
AGGGATTAGAAAGAAAGGAA+AGG - chr4.4:34330993-34331012 MS.gene28926:CDS 35.0%
ATCTCTCGAATGAGTTTCTT+GGG + chr4.4:34330919-34330938 None:intergenic 35.0%
ATGTGAGGGATTAGAAAGAA+AGG - chr4.4:34330988-34331007 MS.gene28926:CDS 35.0%
ATTGTGGAGTTTATCACCAA+AGG - chr4.4:34330781-34330800 MS.gene28926:CDS 35.0%
ATTTCAGTTCCTTAAGCCAA+GGG + chr4.4:34329899-34329918 None:intergenic 35.0%
CAAGAACATTATTGCATGTG+AGG - chr4.4:34330973-34330992 MS.gene28926:CDS 35.0%
CACGAAGTTTCTCAAAATCA+AGG + chr4.4:34330484-34330503 None:intergenic 35.0%
CCAAGCTGATATTGAAAACA+AGG + chr4.4:34331191-34331210 None:intergenic 35.0%
CTGATAGGATACTTAAAGCA+TGG - chr4.4:34330087-34330106 MS.gene28926:CDS 35.0%
GAAACTCATTCGAGAGATTT+AGG - chr4.4:34330921-34330940 MS.gene28926:CDS 35.0%
GAGAAACTTCGTGTTAAAAC+CGG - chr4.4:34330491-34330510 MS.gene28926:CDS 35.0%
GCTATTATTGAAGCAATGGA+AGG - chr4.4:34330233-34330252 MS.gene28926:CDS 35.0%
TCAGGCTATTATTGAAGCAA+TGG - chr4.4:34330229-34330248 MS.gene28926:CDS 35.0%
TCGAATGGAATTCACTTACA+AGG - chr4.4:34330611-34330630 MS.gene28926:CDS 35.0%
TGCAGCACTATTTGATTGTT+TGG - chr4.4:34330883-34330902 MS.gene28926:CDS 35.0%
TTGTAAGTGAATTCCATTCG+AGG + chr4.4:34330612-34330631 None:intergenic 35.0%
TTGTGAGGCATATAGAATGA+AGG - chr4.4:34331126-34331145 MS.gene28926:CDS 35.0%
!!! CCTTGTTTTCAATATCAGCT+TGG - chr4.4:34331188-34331207 MS.gene28926:CDS 35.0%
!!! TTTTCCAATTTGCTGACAAC+AGG + chr4.4:34330461-34330480 None:intergenic 35.0%
AAGAGGTTCATGCAGAGTTA+TGG - chr4.4:34331103-34331122 MS.gene28926:CDS 40.0%
AGGAACTGAAATCTGAGGAA+AGG - chr4.4:34329907-34329926 MS.gene28926:CDS 40.0%
ATATGCTTCGGTTTACTGGA+TGG + chr4.4:34330640-34330659 None:intergenic 40.0%
CAAGATTACAAGCAGCTACA+TGG + chr4.4:34329971-34329990 None:intergenic 40.0%
CAATATCAGCTTGGAGATCT+AGG - chr4.4:34331197-34331216 MS.gene28926:CDS 40.0%
CAATCCTGTTGTCAGCAAAT+TGG - chr4.4:34330454-34330473 MS.gene28926:CDS 40.0%
CATCATGGAGAAGAGCTTTA+AGG + chr4.4:34329847-34329866 None:intergenic 40.0%
CCAGTAAACCGAAGCATATT+CGG - chr4.4:34330641-34330660 MS.gene28926:CDS 40.0%
GAAACTTCGTGTTAAAACCG+GGG - chr4.4:34330493-34330512 MS.gene28926:CDS 40.0%
GAACTGAAATCTGAGGAAAG+GGG - chr4.4:34329909-34329928 MS.gene28926:CDS 40.0%
GAGTTTATCACCAAAGGTGA+TGG - chr4.4:34330787-34330806 MS.gene28926:CDS 40.0%
GATTTCAGTTCCTTAAGCCA+AGG + chr4.4:34329900-34329919 None:intergenic 40.0%
GCAAATTGCCGAATATGCTT+CGG + chr4.4:34330652-34330671 None:intergenic 40.0%
GCTTAAGGAACTGAAATCTG+AGG - chr4.4:34329902-34329921 MS.gene28926:CDS 40.0%
GGAACTGAAATCTGAGGAAA+GGG - chr4.4:34329908-34329927 MS.gene28926:CDS 40.0%
TATCCTATCAGCAAGTGCTT+CGG + chr4.4:34330078-34330097 None:intergenic 40.0%
TCTGTTACAACCATCACCTT+TGG + chr4.4:34330800-34330819 None:intergenic 40.0%
TGAACTTGAGTCAGGACTTA+TGG + chr4.4:34330702-34330721 None:intergenic 40.0%
TGACTCAAGTTCATCATCAC+CGG - chr4.4:34330709-34330728 MS.gene28926:CDS 40.0%
TGAGGCATATAGAATGAAGG+AGG - chr4.4:34331129-34331148 MS.gene28926:CDS 40.0%
TGCTAATATGCTGGCAAGAT+AGG - chr4.4:34331164-34331183 MS.gene28926:CDS 40.0%
TGCTGACAACAGGATTGAAT+TGG + chr4.4:34330451-34330470 None:intergenic 40.0%
TTCATCATCCCAGGCTAAAA+GGG + chr4.4:34330555-34330574 None:intergenic 40.0%
TTGCACAAGTGAGCAACAAA+TGG + chr4.4:34329947-34329966 None:intergenic 40.0%
TTGGTAAGTTGCAAGCAAAG+AGG - chr4.4:34331086-34331105 MS.gene28926:CDS 40.0%
TTTCATCATCCCAGGCTAAA+AGG + chr4.4:34330556-34330575 None:intergenic 40.0%
! AAAGGAAAGGCATGAAAAGC+TGG - chr4.4:34331006-34331025 MS.gene28926:CDS 40.0%
! AATTGCATACCCTTTTAGCC+TGG - chr4.4:34330543-34330562 MS.gene28926:CDS 40.0%
! AGGCTAAAAGGGTATGCAAT+TGG + chr4.4:34330544-34330563 None:intergenic 40.0%
! AGGGTGGAGAAACTTCATTT+TGG - chr4.4:34330941-34330960 MS.gene28926:CDS 40.0%
! ATTGCATACCCTTTTAGCCT+GGG - chr4.4:34330544-34330563 MS.gene28926:CDS 40.0%
!! GGAGAAACTTCATTTTGGTG+AGG - chr4.4:34330946-34330965 MS.gene28926:CDS 40.0%
AGAAGTGAAATCCACTGAGC+AGG + chr4.4:34330299-34330318 None:intergenic 45.0%
AGGATACTTAAAGCATGGCC+TGG - chr4.4:34330092-34330111 MS.gene28926:CDS 45.0%
AGTGTAGAGCTTCTAGTAGC+CGG + chr4.4:34330859-34330878 None:intergenic 45.0%
ATGGCTGTGTGCTAATATGC+TGG - chr4.4:34331155-34331174 MS.gene28926:CDS 45.0%
CAATCAGTCTATGAGCTACC+TGG + chr4.4:34330400-34330419 None:intergenic 45.0%
CAGGATGAGCACTCAAAACT+TGG + chr4.4:34330322-34330341 None:intergenic 45.0%
CATGCAGAGTTATGGTTGTG+AGG - chr4.4:34331111-34331130 MS.gene28926:CDS 45.0%
CCGAATATGCTTCGGTTTAC+TGG + chr4.4:34330644-34330663 None:intergenic 45.0%
CCTCCACATTTGAGAATCAC+CGG - chr4.4:34330347-34330366 MS.gene28926:CDS 45.0%
CGGTGATTCTCAAATGTGGA+GGG + chr4.4:34330349-34330368 None:intergenic 45.0%
CTCATTCGAGAGATTTAGGG+TGG - chr4.4:34330925-34330944 MS.gene28926:CDS 45.0%
CTCTTTCTTCTGATGAACGC+CGG + chr4.4:34330369-34330388 None:intergenic 45.0%
CTTCATGGTTTCATCATCCC+AGG + chr4.4:34330564-34330583 None:intergenic 45.0%
GAGTAGAGTTGAGTGCTTTG+TGG + chr4.4:34330121-34330140 None:intergenic 45.0%
GGCATGAAAAGCTGGATAAG+TGG - chr4.4:34331014-34331033 MS.gene28926:CDS 45.0%
TTGAGTGCTTTGTGGATTCC+AGG + chr4.4:34330113-34330132 None:intergenic 45.0%
! ATGGCTCACATTTTACGGAC+CGG - chr4.4:34330837-34330856 MS.gene28926:CDS 45.0%
! GGTGATGATGAACTTGAGTC+AGG + chr4.4:34330710-34330729 None:intergenic 45.0%
! TTACCGAAGCACTTGCTGAT+AGG - chr4.4:34330072-34330091 MS.gene28926:CDS 45.0%
!! GTTTTGAGTGCTCATCCTGA+TGG - chr4.4:34330323-34330342 MS.gene28926:CDS 45.0%
ACGCCGGTGATTCTCAAATG+TGG + chr4.4:34330353-34330372 None:intergenic 50.0%
ACTAATTGCTAGTGCCTCCC+CGG + chr4.4:34330513-34330532 None:intergenic 50.0%
ACTTCGTGTTAAAACCGGGG+AGG - chr4.4:34330496-34330515 MS.gene28926:CDS 50.0%
AGTTCCTTAAGCCAAGGGTG+TGG + chr4.4:34329894-34329913 None:intergenic 50.0%
ATCTCCACACCCTTGGCTTA+AGG - chr4.4:34329887-34329906 MS.gene28926:CDS 50.0%
CCGGTGATTCTCAAATGTGG+AGG + chr4.4:34330350-34330369 None:intergenic 50.0%
CGGCGTTCATCAGAAGAAAG+AGG - chr4.4:34330367-34330386 MS.gene28926:CDS 50.0%
GAAGAAAGAGGTTCTCGACC+AGG - chr4.4:34330379-34330398 MS.gene28926:CDS 50.0%
GATCCAAAGCCAGATGAAGG+TGG + chr4.4:34329870-34329889 None:intergenic 50.0%
GGAGATCCAAAGCCAGATGA+AGG + chr4.4:34329873-34329892 None:intergenic 50.0%
TCCATGATGCCACCTTCATC+TGG - chr4.4:34329858-34329877 MS.gene28926:CDS 50.0%
! ATGCCACCTTCATCTGGCTT+TGG - chr4.4:34329864-34329883 MS.gene28926:CDS 50.0%
!! CAACTCTACTCGAGTGTCGA+TGG - chr4.4:34330130-34330149 MS.gene28926:CDS 50.0%
ATGCTGCTGAACCTGCTCAG+TGG - chr4.4:34330285-34330304 MS.gene28926:CDS 55.0%
CTCAAATGTGGAGGGCCATC+AGG + chr4.4:34330341-34330360 None:intergenic 55.0%
CTGACGGCGATACTATGCAG+CGG - chr4.4:34330036-34330055 MS.gene28926:CDS 55.0%
CTGCATAGTATCGCCGTCAG+GGG + chr4.4:34330036-34330055 None:intergenic 55.0%
GCCAGATGAAGGTGGCATCA+TGG + chr4.4:34329862-34329881 None:intergenic 55.0%
GCTGCATAGTATCGCCGTCA+GGG + chr4.4:34330037-34330056 None:intergenic 55.0%
TCCATGCTTGCTTCCCCTGA+CGG - chr4.4:34330020-34330039 MS.gene28926:CDS 55.0%
! GCTTTGGATCTCCACACCCT+TGG - chr4.4:34329880-34329899 MS.gene28926:CDS 55.0%
CGCTGCATAGTATCGCCGTC+AGG + chr4.4:34330038-34330057 None:intergenic 60.0%
GCCGTCAGGGGAAGCAAGCA+TGG + chr4.4:34330024-34330043 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 34329807 34331225 34329807 ID=MS.gene28926
chr4.4 mRNA 34329807 34331225 34329807 ID=MS.gene28926.t1;Parent=MS.gene28926
chr4.4 exon 34329807 34331225 34329807 ID=MS.gene28926.t1.exon1;Parent=MS.gene28926.t1
chr4.4 CDS 34329807 34331225 34329807 ID=cds.MS.gene28926.t1;Parent=MS.gene28926.t1
Gene Sequence

>MS.gene28926

ATGTTGCAAGAAGGGTCATCTTCTGTTACTTCTTCAGCCTTAAAGCTCTTCTCCATGATGCCACCTTCATCTGGCTTTGGATCTCCACACCCTTGGCTTAAGGAACTGAAATCTGAGGAAAGGGGTTTGTACTTGATCCATTTGTTGCTCACTTGTGCAAACCATGTAGCTGCTTGTAATCTTGAGCATGCAAATGCAACACTTGAGCAAATTTCCATGCTTGCTTCCCCTGACGGCGATACTATGCAGCGGATTGCAGCGTATTTTACCGAAGCACTTGCTGATAGGATACTTAAAGCATGGCCTGGAATCCACAAAGCACTCAACTCTACTCGAGTGTCGATGGTTTCAGAAAAGATTCTTGTGCAGAAGCTCTTTTTTGAGCTTTTCCCTTTCATGAAAGTAGCATTTGTTCTCACAAATCAGGCTATTATTGAAGCAATGGAAGGGGAGAGAATGATTCACATAATTGATCTCAATGCTGCTGAACCTGCTCAGTGGATTTCACTTCTCCAAGTTTTGAGTGCTCATCCTGATGGCCCTCCACATTTGAGAATCACCGGCGTTCATCAGAAGAAAGAGGTTCTCGACCAGGTAGCTCATAGACTGATTGCAGAAGCTGAAAAATTAGATATACCATTCCAATTCAATCCTGTTGTCAGCAAATTGGAAAACCTTGATTTTGAGAAACTTCGTGTTAAAACCGGGGAGGCACTAGCAATTAGTTCAATTCTCCAATTGCATACCCTTTTAGCCTGGGATGATGAAACCATGAAGAAAAAATCTCCTCTTTTGTTAAAAACCTCGAATGGAATTCACTTACAAGGATTCCATCCAGTAAACCGAAGCATATTCGGCAATTTGCTTGAGAAAGATTTGGTTAGCAGCTATACCATAAGTCCTGACTCAAGTTCATCATCACCGGTTTCTTTGAACACTTCAAATTCAATGAACACAGAGACATTTCTCAATGCATTGTGGAGTTTATCACCAAAGGTGATGGTTGTAACAGAACAAGACTCTAATCATAATGGCTCACATTTTACGGACCGGCTACTAGAAGCTCTACACTCTTATGCAGCACTATTTGATTGTTTGGAATCAACGGTCCCAAGAAACTCATTCGAGAGATTTAGGGTGGAGAAACTTCATTTTGGTGAGGAAATCAAGAACATTATTGCATGTGAGGGATTAGAAAGAAAGGAAAGGCATGAAAAGCTGGATAAGTGGTTTATGAGATTTGATTTAGCTGGATTTGGTAATGAGCCTCTAAGTTATTTTGGTAAGTTGCAAGCAAAGAGGTTCATGCAGAGTTATGGTTGTGAGGCATATAGAATGAAGGAGGAAAATGGCTGTGTGCTAATATGCTGGCAAGATAGGTCCTTGTTTTCAATATCAGCTTGGAGATCTAGGAAGTAA
Protein sequence

>MS.gene28926.t1

MLQEGSSSVTSSALKLFSMMPPSSGFGSPHPWLKELKSEERGLYLIHLLLTCANHVAACNLEHANATLEQISMLASPDGDTMQRIAAYFTEALADRILKAWPGIHKALNSTRVSMVSEKILVQKLFFELFPFMKVAFVLTNQAIIEAMEGERMIHIIDLNAAEPAQWISLLQVLSAHPDGPPHLRITGVHQKKEVLDQVAHRLIAEAEKLDIPFQFNPVVSKLENLDFEKLRVKTGEALAISSILQLHTLLAWDDETMKKKSPLLLKTSNGIHLQGFHPVNRSIFGNLLEKDLVSSYTISPDSSSSSPVSLNTSNSMNTETFLNALWSLSPKVMVVTEQDSNHNGSHFTDRLLEALHSYAALFDCLESTVPRNSFERFRVEKLHFGEEIKNIIACEGLERKERHEKLDKWFMRFDLAGFGNEPLSYFGKLQAKRFMQSYGCEAYRMKEENGCVLICWQDRSLFSISAWRSRK