AlfalfaGEDB | MS.gene052541

M. sativa cultivar XinJiangDaYe / MS.gene052541

KEGG: K12184
GO: GO:0000813 GO:0005575 GO:0006810 GO:0008150 GO:0016192 GO:0016197 GO:0032509 GO:0032991 GO:0036452 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0071985 GO:0098796
PFAM: PF03997.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052541.t1	XP_003608217.1	99.5	210	1	0	1	210	1	210	4.40E-111	410.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052541.t1	Q9S9T7	83.3	209	35	0	1	209	1	209	3.4e-96	352.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052541.t1	G7JRS3	99.5	210	1	0	1	210	1	210	3.1e-111	410.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052541	MS.gene32191	0.952925	8.43E-111	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene33737	MS.gene052541	PPI
MS.gene052541	MS.gene035218	PPI
MS.gene32191	MS.gene052541	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052541.t1	MTR_4g090890	99.524	210	1	0	1	210	1	210	6.14e-159	437

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene052541.t1	AT4G05000	83.254	209	35	1	209	1	209	9.96e-135	376
MS.gene052541.t1	AT4G05000	83.254	209	35	1	209	1	209	9.96e-135	376
MS.gene052541.t1	AT4G05000	83.254	209	35	1	209	1	209	9.96e-135	376
MS.gene052541.t1	AT4G21560	83.173	208	35	3	210	2	209	2.76e-126	355
MS.gene052541.t1	AT4G21560	83.173	208	35	3	210	2	209	2.76e-126	355
MS.gene052541.t1	AT4G21560	83.173	208	35	3	210	2	209	2.76e-126	355

Find 52 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTTTGCTCTCAGATCTTTA+TGG	0.115170	4.3:-23170430	MS.gene052541:CDS
GTCTTCTTACAATTCCTTTA+TGG	0.172521	4.3:-23170266	MS.gene052541:CDS
CACTCTATCTCATATTCCTT+TGG	0.322658	4.3:+23170724	None:intergenic
TGCTAGGTACAGTGTCCTTA+AGG	0.356333	4.3:+23170662	None:intergenic
TGAAATGAGCAATTAGCTTC+TGG	0.384000	4.3:+23170701	None:intergenic
ATTAACCGTCTTGTGGTATC+TGG	0.384677	4.3:-23170595	MS.gene052541:CDS
AACCCTAGCTTCCACCCTTA+AGG	0.408396	4.3:-23170677	MS.gene052541:CDS
AGCCACCATGTTAAGTTTCA+AGG	0.416540	4.3:+23170468	None:intergenic
TGCCTGCTACAGTGGAGCAC+CGG	0.419657	4.3:-23170570	MS.gene052541:CDS
ATTGGGCAGAGCTGCCATAA+AGG	0.420324	4.3:+23170252	None:intergenic
TACCTATAGGATGGAGTGCC+CGG	0.420784	4.3:-23170623	MS.gene052541:CDS
AGAGATTTGCTGATACCTAT+AGG	0.426552	4.3:-23170636	MS.gene052541:CDS
TGATGAATTGACAGAACAAC+AGG	0.433481	4.3:-23170314	MS.gene052541:CDS
GATTGGAAGGTTATCAAAGA+TGG	0.440404	4.3:-23170344	MS.gene052541:CDS
TAACATGGTGGCTGTGGATC+AGG	0.449737	4.3:-23170458	MS.gene052541:CDS
TGTCCTTAAGGGTGGAAGCT+AGG	0.450461	4.3:+23170674	None:intergenic
CACCGGCTGCTGCAGTAGCC+CGG	0.452909	4.3:+23170551	None:intergenic
AAGATCAAAGTGAAGCTGTC+TGG	0.458152	4.3:+23170291	None:intergenic
ATGGCAGCTCTGCCCAATGC+TGG	0.461234	4.3:-23170247	MS.gene052541:CDS
CTCAGTCAAGTGCCAGCATT+GGG	0.462909	4.3:+23170235	None:intergenic
CACCGGGCTACTGCAGCAGC+CGG	0.465917	4.3:-23170553	MS.gene052541:CDS
ATCTGGTGTGCCTGCTACAG+TGG	0.466901	4.3:-23170578	MS.gene052541:CDS
GCCCGGTGCTCCACTGTAGC+AGG	0.469416	4.3:+23170568	None:intergenic
GTCCTTAAGGGTGGAAGCTA+GGG	0.470135	4.3:+23170675	None:intergenic
GCTAGGTACAGTGTCCTTAA+GGG	0.471651	4.3:+23170663	None:intergenic
TGAGCTTTATGCAATTATCA+AGG	0.477470	4.3:-23170791	MS.gene052541:CDS
GCCTGCTACAGTGGAGCACC+GGG	0.503675	4.3:-23170569	MS.gene052541:CDS
GAAACTTAACATGGTGGCTG+TGG	0.505970	4.3:-23170464	MS.gene052541:CDS
ACTCAGTCAAGTGCCAGCAT+TGG	0.506723	4.3:+23170234	None:intergenic
AGCCGGGCACTCCATCCTAT+AGG	0.544884	4.3:+23170621	None:intergenic
ACAAGACGGTTAATCGCAGC+CGG	0.551667	4.3:+23170604	None:intergenic
AAATGAAGGATTGGATTGGA+AGG	0.553711	4.3:-23170357	MS.gene052541:CDS
ACATGACGGAGGTTAAGCTC+TGG	0.557097	4.3:-23170852	MS.gene052541:CDS
TCAGCAAATCTCTCAATGCT+AGG	0.562437	4.3:+23170646	None:intergenic
ATTGGAAGGTTATCAAAGAT+GGG	0.567981	4.3:-23170343	MS.gene052541:CDS
ATTTGCTGATACCTATAGGA+TGG	0.575681	4.3:-23170632	MS.gene052541:CDS
CTCAGCCACAGTGGCAGCCG+AGG	0.584044	4.3:+23170522	None:intergenic
GGCAGCCGAGGTGGACACAC+CGG	0.587917	4.3:+23170534	None:intergenic
GTCCACCTCGGCTGCCACTG+TGG	0.597134	4.3:-23170527	MS.gene052541:CDS
AGCAGCCGGTGTGTCCACCT+CGG	0.599861	4.3:-23170539	MS.gene052541:CDS
CCATAAAGATCTGAGAGCAA+AGG	0.601434	4.3:+23170430	None:intergenic
TAGAGATATAATATCACCAA+AGG	0.609606	4.3:-23170740	MS.gene052541:CDS
AGCCACAGTGGCAGCCGAGG+TGG	0.627242	4.3:+23170525	None:intergenic
AGGTACAGTGTCCTTAAGGG+TGG	0.630756	4.3:+23170666	None:intergenic
GGATTCCTTGAAACTTAACA+TGG	0.637083	4.3:-23170473	MS.gene052541:CDS
CAAGACGGTTAATCGCAGCC+GGG	0.658456	4.3:+23170605	None:intergenic
CAATTATCAAGGCTACTGAA+AGG	0.659301	4.3:-23170780	MS.gene052541:CDS
GGCTGCGATTAACCGTCTTG+TGG	0.665895	4.3:-23170602	MS.gene052541:CDS
TAGAATAAATAACATGACGG+AGG	0.669493	4.3:-23170863	None:intergenic
CTGAACACACTCAGCCACAG+TGG	0.699627	4.3:+23170513	None:intergenic
TTCCTTGAAACTTAACATGG+TGG	0.700241	4.3:-23170470	MS.gene052541:CDS
GCACACCAGATACCACAAGA+CGG	0.739666	4.3:+23170590	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATAAAAATGAAGGATTGGAT+TGG	-	chr4.3:23170729-23170748	MS.gene052541:CDS	25.0%
!	GGAAAATAAAAATGAAGGAT+TGG	-	chr4.3:23170724-23170743	MS.gene052541:CDS	25.0%
!	TAGAGATATAATATCACCAA+AGG	-	chr4.3:23170350-23170369	MS.gene052541:CDS	25.0%
!!	TCAGAATTTTATTACATCGA+TGG	-	chr4.3:23170596-23170615	MS.gene052541:CDS	25.0%
	ATTGGAAGGTTATCAAAGAT+GGG	-	chr4.3:23170747-23170766	MS.gene052541:CDS	30.0%
	GTCTTCTTACAATTCCTTTA+TGG	-	chr4.3:23170824-23170843	MS.gene052541:CDS	30.0%
!	TGAGCTTTATGCAATTATCA+AGG	-	chr4.3:23170299-23170318	MS.gene052541:CDS	30.0%
	CAATTATCAAGGCTACTGAA+AGG	-	chr4.3:23170310-23170329	MS.gene052541:CDS	35.0%
	CACTCTATCTCATATTCCTT+TGG	+	chr4.3:23170369-23170388	None:intergenic	35.0%
	CGAGGGGAAAATAAAAATGA+AGG	-	chr4.3:23170719-23170738	MS.gene052541:CDS	35.0%
	GATTGGAAGGTTATCAAAGA+TGG	-	chr4.3:23170746-23170765	MS.gene052541:CDS	35.0%
	GGATTCCTTGAAACTTAACA+TGG	-	chr4.3:23170617-23170636	MS.gene052541:CDS	35.0%
	TGATGAATTGACAGAACAAC+AGG	-	chr4.3:23170776-23170795	MS.gene052541:CDS	35.0%
	TTCCTTGAAACTTAACATGG+TGG	-	chr4.3:23170620-23170639	MS.gene052541:CDS	35.0%
!	AAATGAAGGATTGGATTGGA+AGG	-	chr4.3:23170733-23170752	MS.gene052541:CDS	35.0%
!	ATTTGCTGATACCTATAGGA+TGG	-	chr4.3:23170458-23170477	MS.gene052541:CDS	35.0%
!	TGAAATGAGCAATTAGCTTC+TGG	+	chr4.3:23170392-23170411	None:intergenic	35.0%
!!	AGAGATTTGCTGATACCTAT+AGG	-	chr4.3:23170454-23170473	MS.gene052541:CDS	35.0%
!!	TTTATTTTCCCCTCGAAATC+AGG	+	chr4.3:23170714-23170733	None:intergenic	35.0%
	AAGATCAAAGTGAAGCTGTC+TGG	+	chr4.3:23170802-23170821	None:intergenic	40.0%
	AGCCACCATGTTAAGTTTCA+AGG	+	chr4.3:23170625-23170644	None:intergenic	40.0%
	ATTAACCGTCTTGTGGTATC+TGG	-	chr4.3:23170495-23170514	MS.gene052541:CDS	40.0%
	CCATAAAGATCTGAGAGCAA+AGG	+	chr4.3:23170663-23170682	None:intergenic	40.0%
	CCTTTGCTCTCAGATCTTTA+TGG	-	chr4.3:23170660-23170679	MS.gene052541:CDS	40.0%
	TCAGCAAATCTCTCAATGCT+AGG	+	chr4.3:23170447-23170466	None:intergenic	40.0%
	GAAACTTAACATGGTGGCTG+TGG	-	chr4.3:23170626-23170645	MS.gene052541:CDS	45.0%
	GCTAGGTACAGTGTCCTTAA+GGG	+	chr4.3:23170430-23170449	None:intergenic	45.0%
	TGCTAGGTACAGTGTCCTTA+AGG	+	chr4.3:23170431-23170450	None:intergenic	45.0%
!	ATTTTCCCCTCGAAATCAGG+CGG	+	chr4.3:23170711-23170730	None:intergenic	45.0%
!	ATTTTGCCGCCTGATTTCGA+GGG	-	chr4.3:23170702-23170721	MS.gene052541:CDS	45.0%
	AACCCTAGCTTCCACCCTTA+AGG	-	chr4.3:23170413-23170432	MS.gene052541:CDS	50.0%
	ACAAGACGGTTAATCGCAGC+CGG	+	chr4.3:23170489-23170508	None:intergenic	50.0%
	AGGTACAGTGTCCTTAAGGG+TGG	+	chr4.3:23170427-23170446	None:intergenic	50.0%
	ATTGGGCAGAGCTGCCATAA+AGG	+	chr4.3:23170841-23170860	None:intergenic	50.0%
	GCACACCAGATACCACAAGA+CGG	+	chr4.3:23170503-23170522	None:intergenic	50.0%
	GTCCTTAAGGGTGGAAGCTA+GGG	+	chr4.3:23170418-23170437	None:intergenic	50.0%
	TAACATGGTGGCTGTGGATC+AGG	-	chr4.3:23170632-23170651	MS.gene052541:CDS	50.0%
	TGTCCTTAAGGGTGGAAGCT+AGG	+	chr4.3:23170419-23170438	None:intergenic	50.0%
!	GATTTTGCCGCCTGATTTCG+AGG	-	chr4.3:23170701-23170720	MS.gene052541:CDS	50.0%
!	TACCTATAGGATGGAGTGCC+CGG	-	chr4.3:23170467-23170486	MS.gene052541:CDS	50.0%
!	TTTTGCCGCCTGATTTCGAG+GGG	-	chr4.3:23170703-23170722	MS.gene052541:CDS	50.0%
	ATCTGGTGTGCCTGCTACAG+TGG	-	chr4.3:23170512-23170531	MS.gene052541:CDS	55.0%
	CAAGACGGTTAATCGCAGCC+GGG	+	chr4.3:23170488-23170507	None:intergenic	55.0%
	CTGAACACACTCAGCCACAG+TGG	+	chr4.3:23170580-23170599	None:intergenic	55.0%
	GGCTGCGATTAACCGTCTTG+TGG	-	chr4.3:23170488-23170507	MS.gene052541:CDS	55.0%
	AGCCGGGCACTCCATCCTAT+AGG	+	chr4.3:23170472-23170491	None:intergenic	60.0%
	ATGGCAGCTCTGCCCAATGC+TGG	-	chr4.3:23170843-23170862	MS.gene052541:CDS	60.0%
	TGCCTGCTACAGTGGAGCAC+CGG	-	chr4.3:23170520-23170539	MS.gene052541:CDS	60.0%
	AGCAGCCGGTGTGTCCACCT+CGG	-	chr4.3:23170551-23170570	MS.gene052541:CDS	65.0%
!	GCCTGCTACAGTGGAGCACC+GGG	-	chr4.3:23170521-23170540	MS.gene052541:CDS	65.0%
	AGCCACAGTGGCAGCCGAGG+TGG	+	chr4.3:23170568-23170587	None:intergenic	70.0%
	CACCGGGCTACTGCAGCAGC+CGG	-	chr4.3:23170537-23170556	MS.gene052541:CDS	70.0%
	CTCAGCCACAGTGGCAGCCG+AGG	+	chr4.3:23170571-23170590	None:intergenic	70.0%
	GCCCGGTGCTCCACTGTAGC+AGG	+	chr4.3:23170525-23170544	None:intergenic	70.0%
	GGCAGCCGAGGTGGACACAC+CGG	+	chr4.3:23170559-23170578	None:intergenic	70.0%
	GTCCACCTCGGCTGCCACTG+TGG	-	chr4.3:23170563-23170582	MS.gene052541:CDS	70.0%
!	CACCGGCTGCTGCAGTAGCC+CGG	+	chr4.3:23170542-23170561	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	23170240	23170872	23170240	ID=MS.gene052541
chr4.3	mRNA	23170240	23170872	23170240	ID=MS.gene052541.t1;Parent=MS.gene052541
chr4.3	exon	23170240	23170872	23170240	ID=MS.gene052541.t1.exon1;Parent=MS.gene052541.t1
chr4.3	CDS	23170240	23170872	23170240	ID=cds.MS.gene052541.t1;Parent=MS.gene052541.t1

Gene Sequence

>MS.gene052541

ATGACGGAGGTTAAGCTCTGGAATGACAAGCGCGAGAGAGAAATGTATGACAACTTTGCTGAGCTTTATGCAATTATCAAGGCTACTGAAAGGCTTGAGAAAGCTTATGTTAGAGATATAATATCACCAAAGGAATATGAGATAGAGTGCCAGAAGCTAATTGCTCATTTCAAAACCCTAGCTTCCACCCTTAAGGACACTGTACCTAGCATTGAGAGATTTGCTGATACCTATAGGATGGAGTGCCCGGCTGCGATTAACCGTCTTGTGGTATCTGGTGTGCCTGCTACAGTGGAGCACCGGGCTACTGCAGCAGCCGGTGTGTCCACCTCGGCTGCCACTGTGGCTGAGTGTGTTCAGAATTTTATTACATCGATGGATTCCTTGAAACTTAACATGGTGGCTGTGGATCAGGTGCATCCTTTGCTCTCAGATCTTTATGGTTCACTCAATAAATTGACGATTTTGCCGCCTGATTTCGAGGGGAAAATAAAAATGAAGGATTGGATTGGAAGGTTATCAAAGATGGGTGCAGCTGATGAATTGACAGAACAACAGGCCAGACAGCTTCACTTTGATCTTGAGTCTTCTTACAATTCCTTTATGGCAGCTCTGCCCAATGCTGGCACTTGA

Protein sequence

>MS.gene052541.t1

MTEVKLWNDKREREMYDNFAELYAIIKATERLEKAYVRDIISPKEYEIECQKLIAHFKTLASTLKDTVPSIERFADTYRMECPAAINRLVVSGVPATVEHRATAAAGVSTSAATVAECVQNFITSMDSLKLNMVAVDQVHPLLSDLYGSLNKLTILPPDFEGKIKMKDWIGRLSKMGAADELTEQQARQLHFDLESSYNSFMAALPNAGT