AlfalfaGEDB | MS.gene32191

M. sativa cultivar XinJiangDaYe / MS.gene32191

KEGG: K12184
GO: GO:0000813 GO:0005575 GO:0006810 GO:0008150 GO:0016192 GO:0016197 GO:0032509 GO:0032991 GO:0036452 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0071985 GO:0098796
PFAM: PF03997.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32191.t1	XP_003608217.1	99.5	210	1	0	1	210	1	210	4.40E-111	410.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32191.t1	Q9S9T7	83.3	209	35	0	1	209	1	209	3.4e-96	352.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32191.t1	G7JRS3	99.5	210	1	0	1	210	1	210	3.1e-111	410.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049297	MS.gene32191	-0.801809	7.56E-49	-1.69E-46
MS.gene050787	MS.gene32191	-0.812331	4.47E-51	-1.69E-46
MS.gene052541	MS.gene32191	0.952925	8.43E-111	-1.69E-46
MS.gene054473	MS.gene32191	-0.804401	2.20E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene32191	MS.gene052541	PPI
MS.gene32191	MS.gene035218	PPI
MS.gene32191	MS.gene33737	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32191.t1	MTR_4g090890	99.524	210	1	0	1	210	1	210	6.14e-159	437

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene32191.t1	AT4G05000	83.254	209	35	1	209	1	209	9.96e-135	376
MS.gene32191.t1	AT4G05000	83.254	209	35	1	209	1	209	9.96e-135	376
MS.gene32191.t1	AT4G05000	83.254	209	35	1	209	1	209	9.96e-135	376
MS.gene32191.t1	AT4G21560	83.173	208	35	3	210	2	209	2.76e-126	355
MS.gene32191.t1	AT4G21560	83.173	208	35	3	210	2	209	2.76e-126	355
MS.gene32191.t1	AT4G21560	83.173	208	35	3	210	2	209	2.76e-126	355

Find 52 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTTTGCTCTCAGATCTTTA+TGG	0.115170	4.4:-22886862	MS.gene32191:CDS
GTCTTCTTACAATTCCTTTA+TGG	0.166564	4.4:-22886698	MS.gene32191:CDS
CACTCTATCTCATATTCCTT+TGG	0.322658	4.4:+22887156	None:intergenic
TGCTAGGTACAGTGTCCTTA+AGG	0.356333	4.4:+22887094	None:intergenic
TGAAATGAGCAATTAGCTTC+TGG	0.384000	4.4:+22887133	None:intergenic
ATTAACCGTCTTGTGGTATC+TGG	0.384677	4.4:-22887027	MS.gene32191:CDS
AACCCTAGCTTCCACCCTTA+AGG	0.408396	4.4:-22887109	MS.gene32191:CDS
AGCCACCATGTTAAGTTTCA+AGG	0.416540	4.4:+22886900	None:intergenic
TGCCTGCTACAGTGGAGCAC+CGG	0.419657	4.4:-22887002	MS.gene32191:CDS
ATTGGGCAGAGCTGCCATAA+AGG	0.420324	4.4:+22886684	None:intergenic
TACCTATAGGATGGAGTGCC+CGG	0.420784	4.4:-22887055	MS.gene32191:CDS
AGAGATTTGCTGATACCTAT+AGG	0.426552	4.4:-22887068	MS.gene32191:CDS
TGATGAATTGACAGAACAAC+AGG	0.433481	4.4:-22886746	MS.gene32191:CDS
GATTGGAAGGTTATCAAAGA+TGG	0.440404	4.4:-22886776	MS.gene32191:CDS
TAACATGGTGGCTGTGGATC+AGG	0.449737	4.4:-22886890	MS.gene32191:CDS
TGTCCTTAAGGGTGGAAGCT+AGG	0.450461	4.4:+22887106	None:intergenic
CACCGGCTGCTGCAGTAGCC+CGG	0.452909	4.4:+22886983	None:intergenic
ATGGCAGCTCTGCCCAATGC+TGG	0.461234	4.4:-22886679	MS.gene32191:CDS
CACCGGGCTACTGCAGCAGC+CGG	0.465917	4.4:-22886985	MS.gene32191:CDS
GAGATCAAAGTGAAGCTGTC+TGG	0.466167	4.4:+22886723	None:intergenic
ATCTGGTGTGCCTGCTACAG+TGG	0.466901	4.4:-22887010	MS.gene32191:CDS
GCCCGGTGCTCCACTGTAGC+AGG	0.469416	4.4:+22887000	None:intergenic
GTCCTTAAGGGTGGAAGCTA+GGG	0.470135	4.4:+22887107	None:intergenic
GCTAGGTACAGTGTCCTTAA+GGG	0.471651	4.4:+22887095	None:intergenic
ATCAGTCAAGTGCCAGCATT+GGG	0.475748	4.4:+22886667	None:intergenic
TGAGCTTTATGCAATTATCA+AGG	0.477470	4.4:-22887223	MS.gene32191:CDS
GCCTGCTACAGTGGAGCACC+GGG	0.503675	4.4:-22887001	MS.gene32191:CDS
GAAACTTAACATGGTGGCTG+TGG	0.505970	4.4:-22886896	MS.gene32191:CDS
AATCAGTCAAGTGCCAGCAT+TGG	0.532447	4.4:+22886666	None:intergenic
AGCCGGGCACTCCATCCTAT+AGG	0.544884	4.4:+22887053	None:intergenic
ACAAGACGGTTAATCGCAGC+CGG	0.551667	4.4:+22887036	None:intergenic
AAATGAAGGATTGGATTGGA+AGG	0.553711	4.4:-22886789	MS.gene32191:CDS
ACATGACGGAGGTTAAGCTC+TGG	0.557097	4.4:-22887284	MS.gene32191:CDS
TCAGCAAATCTCTCAATGCT+AGG	0.562437	4.4:+22887078	None:intergenic
ATTGGAAGGTTATCAAAGAT+GGG	0.567981	4.4:-22886775	MS.gene32191:CDS
ATTTGCTGATACCTATAGGA+TGG	0.575681	4.4:-22887064	MS.gene32191:CDS
CTCAGCCACAGTGGCAGCCG+AGG	0.584044	4.4:+22886954	None:intergenic
GGCAGCCGAGGTGGACACAC+CGG	0.587917	4.4:+22886966	None:intergenic
GTCCACCTCGGCTGCCACTG+TGG	0.597134	4.4:-22886959	MS.gene32191:CDS
AGCAGCCGGTGTGTCCACCT+CGG	0.599861	4.4:-22886971	MS.gene32191:CDS
CCATAAAGATCTGAGAGCAA+AGG	0.601434	4.4:+22886862	None:intergenic
TAGAGATATAATATCACCAA+AGG	0.609606	4.4:-22887172	MS.gene32191:CDS
AGCCACAGTGGCAGCCGAGG+TGG	0.627242	4.4:+22886957	None:intergenic
AGGTACAGTGTCCTTAAGGG+TGG	0.630756	4.4:+22887098	None:intergenic
GGATTCCTTGAAACTTAACA+TGG	0.637083	4.4:-22886905	MS.gene32191:CDS
CAAGACGGTTAATCGCAGCC+GGG	0.658456	4.4:+22887037	None:intergenic
CAATTATCAAGGCTACTGAA+AGG	0.659301	4.4:-22887212	MS.gene32191:CDS
GGCTGCGATTAACCGTCTTG+TGG	0.665895	4.4:-22887034	MS.gene32191:CDS
TAGAATAAATAACATGACGG+AGG	0.669493	4.4:-22887295	None:intergenic
CTGAACACACTCAGCCACAG+TGG	0.699627	4.4:+22886945	None:intergenic
TTCCTTGAAACTTAACATGG+TGG	0.700241	4.4:-22886902	MS.gene32191:CDS
GCACACCAGATACCACAAGA+CGG	0.739666	4.4:+22887022	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATAAAAATGAAGGATTGGAT+TGG	-	chr4.4:22887161-22887180	MS.gene32191:CDS	25.0%
!	GGAAAATAAAAATGAAGGAT+TGG	-	chr4.4:22887156-22887175	MS.gene32191:CDS	25.0%
!	TAGAGATATAATATCACCAA+AGG	-	chr4.4:22886782-22886801	MS.gene32191:CDS	25.0%
!!	TCAGAATTTTATTACATCGA+TGG	-	chr4.4:22887028-22887047	MS.gene32191:CDS	25.0%
	ATTGGAAGGTTATCAAAGAT+GGG	-	chr4.4:22887179-22887198	MS.gene32191:CDS	30.0%
	GTCTTCTTACAATTCCTTTA+TGG	-	chr4.4:22887256-22887275	MS.gene32191:CDS	30.0%
!	TGAGCTTTATGCAATTATCA+AGG	-	chr4.4:22886731-22886750	MS.gene32191:CDS	30.0%
	CAATTATCAAGGCTACTGAA+AGG	-	chr4.4:22886742-22886761	MS.gene32191:CDS	35.0%
	CACTCTATCTCATATTCCTT+TGG	+	chr4.4:22886801-22886820	None:intergenic	35.0%
	CGAGGGGAAAATAAAAATGA+AGG	-	chr4.4:22887151-22887170	MS.gene32191:CDS	35.0%
	GATTGGAAGGTTATCAAAGA+TGG	-	chr4.4:22887178-22887197	MS.gene32191:CDS	35.0%
	GGATTCCTTGAAACTTAACA+TGG	-	chr4.4:22887049-22887068	MS.gene32191:CDS	35.0%
	TGATGAATTGACAGAACAAC+AGG	-	chr4.4:22887208-22887227	MS.gene32191:CDS	35.0%
	TTCCTTGAAACTTAACATGG+TGG	-	chr4.4:22887052-22887071	MS.gene32191:CDS	35.0%
!	AAATGAAGGATTGGATTGGA+AGG	-	chr4.4:22887165-22887184	MS.gene32191:CDS	35.0%
!	ATTTGCTGATACCTATAGGA+TGG	-	chr4.4:22886890-22886909	MS.gene32191:CDS	35.0%
!	TGAAATGAGCAATTAGCTTC+TGG	+	chr4.4:22886824-22886843	None:intergenic	35.0%
!!	AGAGATTTGCTGATACCTAT+AGG	-	chr4.4:22886886-22886905	MS.gene32191:CDS	35.0%
!!	TTTATTTTCCCCTCGAAATC+AGG	+	chr4.4:22887146-22887165	None:intergenic	35.0%
	AGCCACCATGTTAAGTTTCA+AGG	+	chr4.4:22887057-22887076	None:intergenic	40.0%
	ATTAACCGTCTTGTGGTATC+TGG	-	chr4.4:22886927-22886946	MS.gene32191:CDS	40.0%
	CCATAAAGATCTGAGAGCAA+AGG	+	chr4.4:22887095-22887114	None:intergenic	40.0%
	CCTTTGCTCTCAGATCTTTA+TGG	-	chr4.4:22887092-22887111	MS.gene32191:CDS	40.0%
	TCAGCAAATCTCTCAATGCT+AGG	+	chr4.4:22886879-22886898	None:intergenic	40.0%
	GAAACTTAACATGGTGGCTG+TGG	-	chr4.4:22887058-22887077	MS.gene32191:CDS	45.0%
	GAGATCAAAGTGAAGCTGTC+TGG	+	chr4.4:22887234-22887253	None:intergenic	45.0%
	GCTAGGTACAGTGTCCTTAA+GGG	+	chr4.4:22886862-22886881	None:intergenic	45.0%
	TGCTAGGTACAGTGTCCTTA+AGG	+	chr4.4:22886863-22886882	None:intergenic	45.0%
!	ATTTTCCCCTCGAAATCAGG+CGG	+	chr4.4:22887143-22887162	None:intergenic	45.0%
!	ATTTTGCCGCCTGATTTCGA+GGG	-	chr4.4:22887134-22887153	MS.gene32191:CDS	45.0%
	AACCCTAGCTTCCACCCTTA+AGG	-	chr4.4:22886845-22886864	MS.gene32191:CDS	50.0%
	ACAAGACGGTTAATCGCAGC+CGG	+	chr4.4:22886921-22886940	None:intergenic	50.0%
	AGGTACAGTGTCCTTAAGGG+TGG	+	chr4.4:22886859-22886878	None:intergenic	50.0%
	ATTGGGCAGAGCTGCCATAA+AGG	+	chr4.4:22887273-22887292	None:intergenic	50.0%
	GCACACCAGATACCACAAGA+CGG	+	chr4.4:22886935-22886954	None:intergenic	50.0%
	GTCCTTAAGGGTGGAAGCTA+GGG	+	chr4.4:22886850-22886869	None:intergenic	50.0%
	TAACATGGTGGCTGTGGATC+AGG	-	chr4.4:22887064-22887083	MS.gene32191:CDS	50.0%
	TGTCCTTAAGGGTGGAAGCT+AGG	+	chr4.4:22886851-22886870	None:intergenic	50.0%
!	GATTTTGCCGCCTGATTTCG+AGG	-	chr4.4:22887133-22887152	MS.gene32191:CDS	50.0%
!	TACCTATAGGATGGAGTGCC+CGG	-	chr4.4:22886899-22886918	MS.gene32191:CDS	50.0%
!	TTTTGCCGCCTGATTTCGAG+GGG	-	chr4.4:22887135-22887154	MS.gene32191:CDS	50.0%
	ATCTGGTGTGCCTGCTACAG+TGG	-	chr4.4:22886944-22886963	MS.gene32191:CDS	55.0%
	CAAGACGGTTAATCGCAGCC+GGG	+	chr4.4:22886920-22886939	None:intergenic	55.0%
	CTGAACACACTCAGCCACAG+TGG	+	chr4.4:22887012-22887031	None:intergenic	55.0%
	GGCTGCGATTAACCGTCTTG+TGG	-	chr4.4:22886920-22886939	MS.gene32191:CDS	55.0%
	AGCCGGGCACTCCATCCTAT+AGG	+	chr4.4:22886904-22886923	None:intergenic	60.0%
	ATGGCAGCTCTGCCCAATGC+TGG	-	chr4.4:22887275-22887294	MS.gene32191:CDS	60.0%
	TGCCTGCTACAGTGGAGCAC+CGG	-	chr4.4:22886952-22886971	MS.gene32191:CDS	60.0%
	AGCAGCCGGTGTGTCCACCT+CGG	-	chr4.4:22886983-22887002	MS.gene32191:CDS	65.0%
!	GCCTGCTACAGTGGAGCACC+GGG	-	chr4.4:22886953-22886972	MS.gene32191:CDS	65.0%
	AGCCACAGTGGCAGCCGAGG+TGG	+	chr4.4:22887000-22887019	None:intergenic	70.0%
	CACCGGGCTACTGCAGCAGC+CGG	-	chr4.4:22886969-22886988	MS.gene32191:CDS	70.0%
	CTCAGCCACAGTGGCAGCCG+AGG	+	chr4.4:22887003-22887022	None:intergenic	70.0%
	GCCCGGTGCTCCACTGTAGC+AGG	+	chr4.4:22886957-22886976	None:intergenic	70.0%
	GGCAGCCGAGGTGGACACAC+CGG	+	chr4.4:22886991-22887010	None:intergenic	70.0%
	GTCCACCTCGGCTGCCACTG+TGG	-	chr4.4:22886995-22887014	MS.gene32191:CDS	70.0%
!	CACCGGCTGCTGCAGTAGCC+CGG	+	chr4.4:22886974-22886993	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	22886672	22887304	22886672	ID=MS.gene32191
chr4.4	mRNA	22886672	22887304	22886672	ID=MS.gene32191.t1;Parent=MS.gene32191
chr4.4	exon	22886672	22887304	22886672	ID=MS.gene32191.t1.exon1;Parent=MS.gene32191.t1
chr4.4	CDS	22886672	22887304	22886672	ID=cds.MS.gene32191.t1;Parent=MS.gene32191.t1

Gene Sequence

>MS.gene32191

ATGACGGAGGTTAAGCTCTGGAATGACAAGCGCGAGAGAGAAATGTATGACAACTTTGCTGAGCTTTATGCAATTATCAAGGCTACTGAAAGGCTTGAGAAAGCTTATGTTAGAGATATAATATCACCAAAGGAATATGAGATAGAGTGCCAGAAGCTAATTGCTCATTTCAAAACCCTAGCTTCCACCCTTAAGGACACTGTACCTAGCATTGAGAGATTTGCTGATACCTATAGGATGGAGTGCCCGGCTGCGATTAACCGTCTTGTGGTATCTGGTGTGCCTGCTACAGTGGAGCACCGGGCTACTGCAGCAGCCGGTGTGTCCACCTCGGCTGCCACTGTGGCTGAGTGTGTTCAGAATTTTATTACATCGATGGATTCCTTGAAACTTAACATGGTGGCTGTGGATCAGGTGCATCCTTTGCTCTCAGATCTTTATGGTTCACTCAATAAATTGACGATTTTGCCGCCTGATTTCGAGGGGAAAATAAAAATGAAGGATTGGATTGGAAGGTTATCAAAGATGGGTGCAGCTGATGAATTGACAGAACAACAGGCCAGACAGCTTCACTTTGATCTCGAGTCTTCTTACAATTCCTTTATGGCAGCTCTGCCCAATGCTGGCACTTGA

Protein sequence

>MS.gene32191.t1

MTEVKLWNDKREREMYDNFAELYAIIKATERLEKAYVRDIISPKEYEIECQKLIAHFKTLASTLKDTVPSIERFADTYRMECPAAINRLVVSGVPATVEHRATAAAGVSTSAATVAECVQNFITSMDSLKLNMVAVDQVHPLLSDLYGSLNKLTILPPDFEGKIKMKDWIGRLSKMGAADELTEQQARQLHFDLESSYNSFMAALPNAGT