Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052862.t1 | XP_013457085.1 | 79.5 | 88 | 18 | 0 | 21 | 108 | 470 | 557 | 2.60E-27 | 131.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052862.t1 | A0A072UP05 | 79.5 | 88 | 18 | 0 | 21 | 108 | 470 | 557 | 1.9e-27 | 131.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052862 | MS.gene72268 | 0.800622 | 1.32E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052862.t1 | MTR_4g089085 | 79.545 | 88 | 18 | 0 | 21 | 108 | 470 | 557 | 8.15e-39 | 136 |
| MS.gene052862.t1 | MTR_2g013350 | 87.500 | 72 | 8 | 1 | 38 | 108 | 478 | 549 | 3.79e-28 | 107 |
| MS.gene052862.t1 | MTR_2g013350 | 87.500 | 72 | 8 | 1 | 38 | 108 | 401 | 472 | 3.99e-28 | 106 |
| MS.gene052862.t1 | MTR_2g013350 | 87.500 | 72 | 8 | 1 | 38 | 108 | 423 | 494 | 4.29e-28 | 106 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052862.t1 | AT4G21660 | 62.500 | 88 | 33 | 0 | 21 | 108 | 473 | 560 | 9.75e-29 | 108 |
| MS.gene052862.t1 | AT4G21660 | 62.500 | 88 | 33 | 0 | 21 | 108 | 487 | 574 | 1.18e-28 | 108 |
| MS.gene052862.t1 | AT4G21660 | 62.500 | 88 | 33 | 0 | 21 | 108 | 567 | 654 | 2.55e-28 | 107 |
| MS.gene052862.t1 | AT1G11520 | 73.684 | 57 | 13 | 1 | 38 | 94 | 142 | 196 | 4.15e-22 | 86.3 |
Find 0 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTATCATATTTTCTGTTA+AGG | + | 31929:424-443 | MS.gene052862:intron | 15.0% |
| ! | GTATTGTATCTTGATAAACT+CGG | + | 31929:571-590 | MS.gene052862:intron | 25.0% |
| AAAAATTTAGCATACGTGTG+TGG | - | 31929:501-520 | MS.gene052861:intergenic | 30.0% | |
| AATACTTGTGAGGGAAAAAA+AGG | - | 31929:556-575 | MS.gene052862:intergenic | 30.0% | |
| ACGATGTTAACATCATTAAC+GGG | - | 31929:868-887 | MS.gene052862:intergenic | 30.0% | |
| ATCAAGATACAATACTTGTG+AGG | - | 31929:566-585 | MS.gene052862:intergenic | 30.0% | |
| CACGATGTTAACATCATTAA+CGG | - | 31929:869-888 | MS.gene052862:intergenic | 30.0% | |
| TAACGTGTTATATTGTTTCC+AGG | + | 31929:721-740 | MS.gene052862:intron | 30.0% | |
| TCAAGATACAATACTTGTGA+GGG | - | 31929:565-584 | MS.gene052862:intergenic | 30.0% | |
| ! | GTTTTTCCTCTTTTTCTTCA+AGG | - | 31929:451-470 | MS.gene052862:intergenic | 30.0% |
| AGAAGACTCACTTGATAAAG+AGG | - | 31929:353-372 | MS.gene052862:intergenic | 35.0% | |
| ATCTGCTAAAAACTATTCCC+AGG | + | 31929:904-923 | MS.gene052862:intron | 35.0% | |
| GTTATCCCCACAAGAAAATA+CGG | - | 31929:705-724 | MS.gene052862:intergenic | 35.0% | |
| TAAGGTCCTTGAAGAAAAAG+AGG | + | 31929:442-461 | MS.gene052862:intron | 35.0% | |
| TATACAACAGAAGTCATACC+TGG | - | 31929:834-853 | MS.gene052862:intergenic | 35.0% | |
| ! | AAATGTGCCGTATTTTCTTG+TGG | + | 31929:695-714 | MS.gene052862:intron | 35.0% |
| ! | AATGTGCCGTATTTTCTTGT+GGG | + | 31929:696-715 | MS.gene052862:intron | 35.0% |
| ! | AGAGTTCTTGCTCCTTTTTA+TGG | + | 31929:667-686 | MS.gene052862:intron | 35.0% |
| ! | AGTTCTTGCTCCTTTTTATG+GGG | + | 31929:669-688 | MS.gene052862:intron | 35.0% |
| ! | GAGTTCTTGCTCCTTTTTAT+GGG | + | 31929:668-687 | MS.gene052862:intron | 35.0% |
| !! | TTCCAGGTTGATTTGCTTAA+AGG | + | 31929:737-756 | MS.gene052862:intron | 35.0% |
| AACCACATAACTGGAGAGAA+TGG | - | 31929:601-620 | MS.gene052861:intergenic | 40.0% | |
| ACTTGATAAAGAGGCCTTTC+AGG | - | 31929:344-363 | MS.gene052862:intergenic | 40.0% | |
| AGGTCAAAAGACAGATAGAG+TGG | + | 31929:757-776 | MS.gene052862:CDS | 40.0% | |
| ATCCATTCTCTCCAGTTATG+TGG | + | 31929:596-615 | MS.gene052862:intron | 40.0% | |
| ATTCTCTCCAGTTATGTGGT+TGG | + | 31929:600-619 | MS.gene052862:intron | 40.0% | |
| CAAGGGAGGAAGAGAAATTA+CGG | + | 31929:937-956 | MS.gene052862:CDS | 40.0% | |
| CACATTTACCCCCCATAAAA+AGG | - | 31929:682-701 | MS.gene052862:intergenic | 40.0% | |
| GACCTTTAAGCAAATCAACC+TGG | - | 31929:742-761 | MS.gene052862:intergenic | 40.0% | |
| TCCCAGGTATAATGAAGCAA+GGG | + | 31929:920-939 | MS.gene052862:intron | 40.0% | |
| TCCCTTGCTTCATTATACCT+GGG | - | 31929:924-943 | MS.gene052862:intergenic | 40.0% | |
| TTCCCAGGTATAATGAAGCA+AGG | + | 31929:919-938 | MS.gene052862:intron | 40.0% | |
| ! | AAAGAGGAAAAACTTGCTCC+TGG | + | 31929:458-477 | MS.gene052862:CDS | 40.0% |
| ! | ATGTGCCGTATTTTCTTGTG+GGG | + | 31929:697-716 | MS.gene052862:intron | 40.0% |
| ! | GTTCTTGCTCCTTTTTATGG+GGG | + | 31929:670-689 | MS.gene052862:intron | 40.0% |
| ! | TGGAGAACGTTTTACATACC+AGG | + | 31929:813-832 | MS.gene052862:CDS | 40.0% |
| ! | TTCTTGCTCCTTTTTATGGG+GGG | + | 31929:671-690 | MS.gene052862:intron | 40.0% |
| AAGACAAGTCAGGGGCAAAA+AGG | + | 31929:634-653 | MS.gene052862:CDS | 45.0% | |
| AGACAAGTCAGGGGCAAAAA+GGG | + | 31929:635-654 | MS.gene052862:CDS | 45.0% | |
| CAGGTATAATGAAGCAAGGG+AGG | + | 31929:923-942 | MS.gene052862:intron | 45.0% | |
| CTCCCTTGCTTCATTATACC+TGG | - | 31929:925-944 | MS.gene052862:intergenic | 45.0% | |
| GTAGTTCCAAGCAAAGTTCC+AGG | - | 31929:479-498 | MS.gene052862:intergenic | 45.0% | |
| !! | TTTTGCCCCTGACTTGTCTT+GGG | - | 31929:634-653 | MS.gene052862:intergenic | 45.0% |
| !! | TTTTTGCCCCTGACTTGTCT+TGG | - | 31929:635-654 | MS.gene052862:intergenic | 45.0% |
| !!! | TATAATTTTTTTATAGTATT+TGG | + | 31929:520-539 | MS.gene052862:intron | 5.0% |
| AGCAGAGGAAGGAACCTGAA+AGG | + | 31929:327-346 | MS.gene052862:CDS | 50.0% | |
| CACTTTACTGCCAGAGGAGT+GGG | + | 31929:784-803 | MS.gene052862:CDS | 50.0% | |
| CTCCAGTTATGTGGTTGGCA+CGG | + | 31929:605-624 | MS.gene052862:intron | 50.0% | |
| CTTGCTCCTGGAACTTTGCT+TGG | + | 31929:470-489 | MS.gene052862:CDS | 50.0% | |
| GAAATTACGGAGTCAGCGTG+AGG | + | 31929:950-969 | MS.gene052862:CDS | 50.0% | |
| GCGTGAGGATTTCAGTGACA+TGG | + | 31929:965-984 | MS.gene052862:CDS | 50.0% | |
| TCACTTTACTGCCAGAGGAG+TGG | + | 31929:783-802 | MS.gene052862:CDS | 50.0% | |
| TCCAGTTATGTGGTTGGCAC+GGG | + | 31929:606-625 | MS.gene052862:intron | 50.0% | |
| TTGACCTTCGTAAGGAGCAG+AGG | + | 31929:312-331 | MS.gene052862:CDS | 50.0% | |
| !! | GGATGTCACTTTACTGCCAG+AGG | + | 31929:778-797 | MS.gene052862:CDS | 50.0% |
| CCTTCCTCTGCTCCTTACGA+AGG | - | 31929:319-338 | MS.gene052862:intergenic | 55.0% | |
| CCTTCGTAAGGAGCAGAGGA+AGG | + | 31929:316-335 | MS.gene052862:CDS | 55.0% | |
| TCCATGACATCCCACTCCTC+TGG | - | 31929:797-816 | MS.gene052862:intergenic | 55.0% | |
| ACGGGCACCCAAGACAAGTC+AGG | + | 31929:624-643 | MS.gene052862:CDS | 60.0% | |
| CGGGCACCCAAGACAAGTCA+GGG | + | 31929:625-644 | MS.gene052862:CDS | 60.0% | |
| GCCAGAGGAGTGGGATGTCA+TGG | + | 31929:793-812 | MS.gene052862:CDS | 60.0% | |
| GCCCGTGCCAACCACATAAC+TGG | - | 31929:610-629 | MS.gene052862:intergenic | 60.0% | |
| GGGCACCCAAGACAAGTCAG+GGG | + | 31929:626-645 | MS.gene052862:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 31929 | gene | 306 | 995 | 306 | ID=MS.gene052862 |
| 31929 | mRNA | 306 | 995 | 306 | ID=MS.gene052862.t1;Parent=MS.gene052862 |
| 31929 | exon | 306 | 361 | 306 | ID=MS.gene052862.t1.exon1;Parent=MS.gene052862.t1 |
| 31929 | CDS | 306 | 361 | 306 | ID=cds.MS.gene052862.t1;Parent=MS.gene052862.t1 |
| 31929 | exon | 446 | 505 | 446 | ID=MS.gene052862.t1.exon2;Parent=MS.gene052862.t1 |
| 31929 | CDS | 446 | 505 | 446 | ID=cds.MS.gene052862.t1;Parent=MS.gene052862.t1 |
| 31929 | exon | 611 | 656 | 611 | ID=MS.gene052862.t1.exon3;Parent=MS.gene052862.t1 |
| 31929 | CDS | 611 | 656 | 611 | ID=cds.MS.gene052862.t1;Parent=MS.gene052862.t1 |
| 31929 | exon | 743 | 834 | 743 | ID=MS.gene052862.t1.exon4;Parent=MS.gene052862.t1 |
| 31929 | CDS | 743 | 834 | 743 | ID=cds.MS.gene052862.t1;Parent=MS.gene052862.t1 |
| 31929 | exon | 926 | 995 | 926 | ID=MS.gene052862.t1.exon5;Parent=MS.gene052862.t1 |
| 31929 | CDS | 926 | 995 | 926 | ID=cds.MS.gene052862.t1;Parent=MS.gene052862.t1 |
| Gene Sequence |
| Protein sequence |
