AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72268


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72268.t1 MTR_1g046070 96.567 233 6 1 1 231 1 233 4.63e-154 427
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72268.t1 AT4G13540 46.061 165 71 4 49 212 37 184 1.60e-32 117
MS.gene72268.t1 AT3G23930 41.279 172 78 4 52 212 40 199 1.29e-23 95.1

Find 50 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCAATCTGCCTCCTTAAA+AGG 0.218161 1.1:+28855869 None:intergenic
TGAGAAAGAGTGGGAATTAA+TGG 0.247721 1.1:-28855735 MS.gene72268:CDS
ACTTACCATCATATGTTCTT+TGG 0.249382 1.1:+28855600 None:intergenic
GAAGAAGTTGAAAATCAAAT+TGG 0.250274 1.1:-28854764 MS.gene72268:CDS
TTCTTCTCTTTAGTTTGAAT+TGG 0.254934 1.1:+28854784 None:intergenic
AAAGCTTTAAAAGCTCAATT+AGG 0.297525 1.1:-28854892 MS.gene72268:CDS
ATTAATGGGGACTAAGCTTT+TGG 0.315398 1.1:-28855720 MS.gene72268:CDS
AAAGGGAATTTGACTTGTTA+AGG 0.331176 1.1:-28854840 MS.gene72268:CDS
TAAAGGAGATAATGTGTGAT+AGG 0.355573 1.1:-28855934 MS.gene72268:CDS
ACTTCTACAAGAAGAAATAA+AGG 0.374088 1.1:-28855951 MS.gene72268:CDS
GAACTATGAGAGGGAGATAA+TGG 0.387809 1.1:-28855897 MS.gene72268:CDS
GAGAAAGAGTGGGAATTAAT+GGG 0.405188 1.1:-28855734 MS.gene72268:CDS
GAGGGAAGTGGTGGAAGAGA+AGG 0.429388 1.1:-28855807 MS.gene72268:CDS
AAGAGAAGGAAGAGAAGATA+AGG 0.430927 1.1:-28855793 MS.gene72268:CDS
AAGAAGTTGAAAATCAAATT+GGG 0.435875 1.1:-28854763 MS.gene72268:CDS
GGTGAAGCAGTTGAGGGAAG+TGG 0.438865 1.1:-28855819 MS.gene72268:CDS
AAGAAGAGGTGAAGCAGTTG+AGG 0.440787 1.1:-28855826 MS.gene72268:CDS
TCACAGGAGATGGTTTGTAT+TGG 0.451338 1.1:-28854984 MS.gene72268:intron
AGAAGAATTGTGAGAAAGAG+TGG 0.454742 1.1:-28855745 MS.gene72268:CDS
ATAAGGGAGATGGAAGAGGT+TGG 0.460472 1.1:-28855776 MS.gene72268:CDS
AAGAAAGGTACAAACAAGAA+AGG 0.469395 1.1:-28854858 MS.gene72268:CDS
AGAGAAGGAAGAGAAGATAA+GGG 0.476340 1.1:-28855792 MS.gene72268:CDS
AGAAGAGGTGAAGCAGTTGA+GGG 0.480459 1.1:-28855825 MS.gene72268:CDS
AAGAGAGTAGGAACTATGAG+AGG 0.487797 1.1:-28855907 MS.gene72268:CDS
GAAGATAAGGGAGATGGAAG+AGG 0.488344 1.1:-28855780 MS.gene72268:CDS
TACAAATTGAAAATCTGAAG+AGG 0.492525 1.1:-28854942 MS.gene72268:CDS
GTGATAGGGAGAAAGAGAGT+AGG 0.501313 1.1:-28855919 MS.gene72268:CDS
GGAAGAGAAGATAAGGGAGA+TGG 0.503969 1.1:-28855786 MS.gene72268:CDS
TCTTGCAATTAAGAATGAGT+TGG 0.505081 1.1:-28855633 MS.gene72268:CDS
TAGGTTCAGTGGAGAAAGAA+AGG 0.512086 1.1:-28854873 MS.gene72268:CDS
GAAGCAGTTGAGGGAAGTGG+TGG 0.513840 1.1:-28855816 MS.gene72268:CDS
AAAAGCTCAATTAGGTTCAG+TGG 0.518823 1.1:-28854884 MS.gene72268:CDS
GAGATGAAGCTGTAGAGAAG+TGG 0.520386 1.1:-28855667 MS.gene72268:CDS
AGAGAGTAGGAACTATGAGA+GGG 0.522297 1.1:-28855906 MS.gene72268:CDS
CTCATCCAAAGAACATATGA+TGG 0.522947 1.1:-28855605 MS.gene72268:intron
AAAGGAGATAATGTGTGATA+GGG 0.526845 1.1:-28855933 MS.gene72268:CDS
AGAAAGGTACAAACAAGAAA+GGG 0.530283 1.1:-28854857 MS.gene72268:CDS
GGAGATGGAAGAGGTTGGAA+TGG 0.546010 1.1:-28855771 MS.gene72268:CDS
ACTTGTTAAGGCAAAGCTTG+AGG 0.554263 1.1:-28854828 MS.gene72268:CDS
CAAAGCTTGAGGATCATGAA+TGG 0.565818 1.1:-28854817 MS.gene72268:CDS
GGTGAAGAGGTTGAAAGAAG+AGG 0.569093 1.1:-28855840 MS.gene72268:CDS
ATTGGAAACAAGAGGTGAAG+AGG 0.596605 1.1:-28855853 MS.gene72268:CDS
ACAAATTGAAAATCTGAAGA+GGG 0.598118 1.1:-28854941 MS.gene72268:CDS
GAAGAATTGTGAGAAAGAGT+GGG 0.599083 1.1:-28855744 MS.gene72268:CDS
AGAAAGAGTGGGAATTAATG+GGG 0.618319 1.1:-28855733 MS.gene72268:CDS
AGATGAAGGAGGAAAGAGCA+AGG 0.618929 1.1:-28855691 MS.gene72268:CDS
TGGTTTGTATTGGAAAGCTG+AGG 0.636251 1.1:-28854974 MS.gene72268:CDS
GGAGGCAGATTGGAAACAAG+AGG 0.643296 1.1:-28855861 MS.gene72268:CDS
TTTGGTTCAAGAGATGAAGG+AGG 0.645416 1.1:-28855702 MS.gene72268:CDS
GGGGCCAATTCAAAGATGGG+GGG 0.731569 1.1:-28856049 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTAAATGAAATTCTTT+TGG + chr1.1:28855474-28855493 None:intergenic 10.0%
!!! AAACTATAAACTGTTTTTAT+AGG + chr1.1:28855448-28855467 None:intergenic 15.0%
!!! AAATTCATTACATATTTTTG+TGG + chr1.1:28854804-28854823 None:intergenic 15.0%
!! AAATACAAACACTAATTGTT+TGG + chr1.1:28855564-28855583 None:intergenic 20.0%
!! AAGAAGTTGAAAATCAAATT+GGG - chr1.1:28856027-28856046 MS.gene72268:CDS 20.0%
!! ATTAATAAGTGACAATAATG+TGG - chr1.1:28855687-28855706 MS.gene72268:CDS 20.0%
!!! AATTTTGAAAAGTACTATGA+TGG + chr1.1:28855662-28855681 None:intergenic 20.0%
!!! ATTTTGAAAAGTACTATGAT+GGG + chr1.1:28855661-28855680 None:intergenic 20.0%
!!! GAGTTATTTTAGCTTATTTA+TGG - chr1.1:28855255-28855274 MS.gene72268:intron 20.0%
! ACAAATTGAAAATCTGAAGA+GGG - chr1.1:28855849-28855868 MS.gene72268:CDS 25.0%
! ACTTCTACAAGAAGAAATAA+AGG - chr1.1:28854839-28854858 MS.gene72268:CDS 25.0%
! GAAGAAGTTGAAAATCAAAT+TGG - chr1.1:28856026-28856045 MS.gene72268:CDS 25.0%
! TACAAATTGAAAATCTGAAG+AGG - chr1.1:28855848-28855867 MS.gene72268:CDS 25.0%
!!! AAAGCTTTAAAAGCTCAATT+AGG - chr1.1:28855898-28855917 MS.gene72268:CDS 25.0%
!!! TTCTTCTCTTTAGTTTGAAT+TGG + chr1.1:28856009-28856028 None:intergenic 25.0%
AAAGGAGATAATGTGTGATA+GGG - chr1.1:28854857-28854876 MS.gene72268:CDS 30.0%
ACTTACCATCATATGTTCTT+TGG + chr1.1:28855193-28855212 None:intergenic 30.0%
TAAAGGAGATAATGTGTGAT+AGG - chr1.1:28854856-28854875 MS.gene72268:CDS 30.0%
TCTTGCAATTAAGAATGAGT+TGG - chr1.1:28855157-28855176 MS.gene72268:intron 30.0%
! AAGAAAGGTACAAACAAGAA+AGG - chr1.1:28855932-28855951 MS.gene72268:CDS 30.0%
! AGAAAGGTACAAACAAGAAA+GGG - chr1.1:28855933-28855952 MS.gene72268:CDS 30.0%
! TACTTGAGCTTATTTGCTTT+TGG - chr1.1:28855775-28855794 MS.gene72268:CDS 30.0%
!! AAAGGGAATTTGACTTGTTA+AGG - chr1.1:28855950-28855969 MS.gene72268:CDS 30.0%
!! GATAATGGTGTTTCCTTTTA+AGG - chr1.1:28854908-28854927 MS.gene72268:CDS 30.0%
AAAAGCTCAATTAGGTTCAG+TGG - chr1.1:28855906-28855925 MS.gene72268:CDS 35.0%
AAGAGAAGGAAGAGAAGATA+AGG - chr1.1:28854997-28855016 MS.gene72268:intron 35.0%
AGAAAGAGTGGGAATTAATG+GGG - chr1.1:28855057-28855076 MS.gene72268:intron 35.0%
AGAAGAATTGTGAGAAAGAG+TGG - chr1.1:28855045-28855064 MS.gene72268:intron 35.0%
AGAGAAGGAAGAGAAGATAA+GGG - chr1.1:28854998-28855017 MS.gene72268:intron 35.0%
CTCATCCAAAGAACATATGA+TGG - chr1.1:28855185-28855204 MS.gene72268:intron 35.0%
GAAGAATTGTGAGAAAGAGT+GGG - chr1.1:28855046-28855065 MS.gene72268:intron 35.0%
GAGAAAGAGTGGGAATTAAT+GGG - chr1.1:28855056-28855075 MS.gene72268:intron 35.0%
TGAGAAAGAGTGGGAATTAA+TGG - chr1.1:28855055-28855074 MS.gene72268:intron 35.0%
! ATTAATGGGGACTAAGCTTT+TGG - chr1.1:28855070-28855089 MS.gene72268:intron 35.0%
!! AATGGTGTTTCCTTTTAAGG+AGG - chr1.1:28854911-28854930 MS.gene72268:CDS 35.0%
!! GCTTTTGGAAATATACTCAC+AGG - chr1.1:28855790-28855809 MS.gene72268:CDS 35.0%
AAAAGTAGTGTGTGGCAGAT+GGG - chr1.1:28855628-28855647 MS.gene72268:CDS 40.0%
AAGAGAGTAGGAACTATGAG+AGG - chr1.1:28854883-28854902 MS.gene72268:CDS 40.0%
AGAGAGTAGGAACTATGAGA+GGG - chr1.1:28854884-28854903 MS.gene72268:CDS 40.0%
ATTGGAAACAAGAGGTGAAG+AGG - chr1.1:28854937-28854956 MS.gene72268:CDS 40.0%
CAAAGCTTGAGGATCATGAA+TGG - chr1.1:28855973-28855992 MS.gene72268:CDS 40.0%
GAACTATGAGAGGGAGATAA+TGG - chr1.1:28854893-28854912 MS.gene72268:CDS 40.0%
GGAAATATACTCACAGGAGA+TGG - chr1.1:28855796-28855815 MS.gene72268:CDS 40.0%
TAGGTTCAGTGGAGAAAGAA+AGG - chr1.1:28855917-28855936 MS.gene72268:CDS 40.0%
TTCCAATCTGCCTCCTTAAA+AGG + chr1.1:28854924-28854943 None:intergenic 40.0%
! ACTTGTTAAGGCAAAGCTTG+AGG - chr1.1:28855962-28855981 MS.gene72268:CDS 40.0%
! TCACAGGAGATGGTTTGTAT+TGG - chr1.1:28855806-28855825 MS.gene72268:CDS 40.0%
! TGGTTTGTATTGGAAAGCTG+AGG - chr1.1:28855816-28855835 MS.gene72268:CDS 40.0%
! TTTGGTTCAAGAGATGAAGG+AGG - chr1.1:28855088-28855107 MS.gene72268:intron 40.0%
!! GCCACACACTACTTTTCAAA+GGG + chr1.1:28855624-28855643 None:intergenic 40.0%
!! TGCCACACACTACTTTTCAA+AGG + chr1.1:28855625-28855644 None:intergenic 40.0%
!! TTCCTTTTAAGGAGGCAGAT+TGG - chr1.1:28854919-28854938 MS.gene72268:CDS 40.0%
!!! GCTTTTGGTTCAAGAGATGA+AGG - chr1.1:28855085-28855104 MS.gene72268:intron 40.0%
AAAGTAGTGTGTGGCAGATG+GGG - chr1.1:28855629-28855648 MS.gene72268:CDS 45.0%
AAGAAGAGGTGAAGCAGTTG+AGG - chr1.1:28854964-28854983 MS.gene72268:CDS 45.0%
AGAAGAGGTGAAGCAGTTGA+GGG - chr1.1:28854965-28854984 MS.gene72268:CDS 45.0%
AGATGAAGGAGGAAAGAGCA+AGG - chr1.1:28855099-28855118 MS.gene72268:intron 45.0%
ATAAGGGAGATGGAAGAGGT+TGG - chr1.1:28855014-28855033 MS.gene72268:intron 45.0%
GAAAAGTAGTGTGTGGCAGA+TGG - chr1.1:28855627-28855646 MS.gene72268:CDS 45.0%
GAAGATAAGGGAGATGGAAG+AGG - chr1.1:28855010-28855029 MS.gene72268:intron 45.0%
GAGATGAAGCTGTAGAGAAG+TGG - chr1.1:28855123-28855142 MS.gene72268:intron 45.0%
GGAAGAGAAGATAAGGGAGA+TGG - chr1.1:28855004-28855023 MS.gene72268:intron 45.0%
GGTGAAGAGGTTGAAAGAAG+AGG - chr1.1:28854950-28854969 MS.gene72268:CDS 45.0%
GTGATAGGGAGAAAGAGAGT+AGG - chr1.1:28854871-28854890 MS.gene72268:CDS 45.0%
! GCCCTTTGAAAAGTAGTGTG+TGG - chr1.1:28855620-28855639 MS.gene72268:CDS 45.0%
GGAGATGGAAGAGGTTGGAA+TGG - chr1.1:28855019-28855038 MS.gene72268:intron 50.0%
GGAGGCAGATTGGAAACAAG+AGG - chr1.1:28854929-28854948 MS.gene72268:CDS 50.0%
GAAGCAGTTGAGGGAAGTGG+TGG - chr1.1:28854974-28854993 MS.gene72268:CDS 55.0%
GGTGAAGCAGTTGAGGGAAG+TGG - chr1.1:28854971-28854990 MS.gene72268:CDS 55.0%
! GAGGGAAGTGGTGGAAGAGA+AGG - chr1.1:28854983-28855002 MS.gene72268:intron 55.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 28854756 28856056 28854756 ID=MS.gene72268
chr1.1 mRNA 28854756 28856056 28854756 ID=MS.gene72268.t1;Parent=MS.gene72268
chr1.1 exon 28855606 28856056 28855606 ID=MS.gene72268.t1.exon1;Parent=MS.gene72268.t1
chr1.1 CDS 28855606 28856056 28855606 ID=cds.MS.gene72268.t1;Parent=MS.gene72268.t1
chr1.1 exon 28854756 28855000 28854756 ID=MS.gene72268.t1.exon2;Parent=MS.gene72268.t1
chr1.1 CDS 28854756 28855000 28854756 ID=cds.MS.gene72268.t1;Parent=MS.gene72268.t1
Gene Sequence

>MS.gene72268

ATGGGGGGAAGTGTTAGTGTTAGCAAGAAAAATGAGATAAAGTATCCACAAAAATATGTAATGAATTTGAAAGAGAAAGTGAGACTTCTACAAGAAGAAATAAAGGAGATAATGTGTGATAGGGAGAAAGAGAGTAGGAACTATGAGAGGGAGATAATGGTGTTTCCTTTTAAGGAGGCAGATTGGAAACAAGAGGTGAAGAGGTTGAAAGAAGAGGTGAAGCAGTTGAGGGAAGTGGTGGAAGAGAAGGAAGAGAAGATAAGGGAGATGGAAGAGGTTGGAATGGTTGAGAAGAATTGTGAGAAAGAGTGGGAATTAATGGGGACTAAGCTTTTGGTTCAAGAGATGAAGGAGGAAAGAGCAAGGAGAGATGAAGCTGTAGAGAAGTGGAAACAACTTTATCTTGCAATTAAGAATGAGTTGGATGATCTCATCCAAAGAACATATGATGGTAAGTTAATCATAATTTGTTACTAGTGTCTTAATACATGTTTGAAATGAGTTATTTTAGCTTATTTATGAGCATGTTTGAGAGTTTATAAAAACAAATTATAACGTGTATAATCTATCTATTTTTATCTTATAAGTTGAATTGAGTTATTTTAGCTTATTTATGGCATTTTTGAGAGAGTTTATAAAAAACAAATTATAATGTGTAATCTATTTTGATCTTAAGTTTTCTAAAATAGCCTATAAAAACAGTTTATAGTTTATGCCAAAAGAATTTCATTTAATTTTTTTTTTTTTTGTAGAATTAGCTTATAAAATAATGATTATATAATAATTGTTTAATTAAGATTTTTATCCAAACAATTAGTGTTTGTATTTTTTTGTGATTTGCATTAGTCTTTGTATACTATTAATGCCCTTTGAAAAGTAGTGTGTGGCAGATGGGGTTCAATCCCATCATAGTACTTTTCAAAATTAGTCAATTAATAAGTGACAATAATGTGGTGAATTATTTCTATTATCTTCTTGAGAAATATTCATACTACTATGCATGAATGCTATGTTTGAACTACTTGAGCTTATTTGCTTTTGGAAATATACTCACAGGAGATGGTTTGTATTGGAAAGCTGAGGAAAATGACATACAAATTGAAAATCTGAAGAGGGAATTGCAAGAGAAAGAAGAAAGCATAAAAGCTTTAAAAGCTCAATTAGGTTCAGTGGAGAAAGAAAGGTACAAACAAGAAAGGGAATTTGACTTGTTAAGGCAAAGCTTGAGGATCATGAATGGCAAGAAGAATCCAATTCAAACTAAAGAGAAGAAGAAGTTGAAAATCAAATTGGGAAAATAA

Protein sequence

>MS.gene72268.t1

MGGSVSVSKKNEIKYPQKYVMNLKEKVRLLQEEIKEIMCDREKESRNYEREIMVFPFKEADWKQEVKRLKEEVKQLREVVEEKEEKIREMEEVGMVEKNCEKEWELMGTKLLVQEMKEERARRDEAVEKWKQLYLAIKNELDDLIQRTYDGDGLYWKAEENDIQIENLKRELQEKEESIKALKAQLGSVEKERYKQEREFDLLRQSLRIMNGKKNPIQTKEKKKLKIKLGK