Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053040.t1 | XP_003627885.2 | 90.2 | 102 | 10 | 0 | 12 | 113 | 203 | 304 | 1.20E-41 | 179.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053040.t1 | G7LGS1 | 90.2 | 102 | 10 | 0 | 12 | 113 | 203 | 304 | 8.5e-42 | 179.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053040 | MS.gene26398 | 0.837371 | 5.41E-57 | -1.69E-46 |
| MS.gene053040 | MS.gene39751 | 0.819103 | 1.38E-52 | -1.69E-46 |
| MS.gene053040 | MS.gene95840 | 0.80013 | 1.67E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053040.t1 | MTR_8g039560 | 90.291 | 103 | 10 | 0 | 12 | 114 | 203 | 305 | 7.59e-60 | 187 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053040.t1 | AT1G08315 | 49.541 | 109 | 46 | 2 | 12 | 111 | 203 | 311 | 1.53e-20 | 84.7 |
Find 30 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGGAGTTTAAGAATGTTTC+TGG | 0.147031 | 6.2:-1125705 | MS.gene053040:CDS |
| ACATCTCTGATATAGGTTGC+TGG | 0.362583 | 6.2:-1125744 | MS.gene053040:intron |
| TTTAAGAATGTTTCTGGTGT+TGG | 0.392345 | 6.2:-1125699 | MS.gene053040:CDS |
| GTTGATGGTTATGTTGAATC+AGG | 0.405976 | 6.2:-1126075 | MS.gene053040:intron |
| AATTTCCAATCCGCTGTTGA+TGG | 0.407173 | 6.2:-1126090 | MS.gene053040:CDS |
| CGCGTTGGCGTTTGGTGCCA+TGG | 0.410736 | 6.2:-1125553 | MS.gene053040:CDS |
| TGGTTGCGAGGAAAGTGTTC+AGG | 0.420933 | 6.2:-1125724 | MS.gene053040:CDS |
| GTAAGAGACGCGTTGGCGTT+TGG | 0.441043 | 6.2:-1125561 | MS.gene053040:CDS |
| TGTTTCTGGTGTTGGAGTGC+TGG | 0.453944 | 6.2:-1125691 | MS.gene053040:CDS |
| AAGTGTTAAGGGGCAGAGTA+AGG | 0.459646 | 6.2:-1125502 | MS.gene053040:CDS |
| TTGGCGTTTGGTGCCATGGA+TGG | 0.501029 | 6.2:-1125549 | MS.gene053040:CDS |
| TGTGGTGGAGATGTGGTGGC+TGG | 0.510838 | 6.2:-1125588 | MS.gene053040:CDS |
| AACATCAGCAATTCCATCCA+TGG | 0.514246 | 6.2:+1125536 | None:intergenic |
| CTGGTTCGAGTACGAGGACG+AGG | 0.519631 | 6.2:-1125647 | MS.gene053040:CDS |
| CATAACCATCAACAGCGGAT+TGG | 0.521661 | 6.2:+1126085 | None:intergenic |
| TGTGAGATGTGGTGGAGATG+TGG | 0.560397 | 6.2:-1125595 | MS.gene053040:CDS |
| CTTAATCTTGTGAGATGTGG+TGG | 0.564928 | 6.2:-1125603 | MS.gene053040:CDS |
| TTGCTTAATCTTGTGAGATG+TGG | 0.567457 | 6.2:-1125606 | MS.gene053040:CDS |
| GATCTACTAGATCTTGCTAC+TGG | 0.569155 | 6.2:-1125666 | MS.gene053040:CDS |
| GACGAGGGAAAATGCAGTGT+CGG | 0.572198 | 6.2:-1125631 | MS.gene053040:CDS |
| GTTGGTGATGTTCGTAGTCA+CGG | 0.574429 | 6.2:-1125456 | MS.gene053040:CDS |
| TTGCTACTGGTTCGAGTACG+AGG | 0.579153 | 6.2:-1125653 | MS.gene053040:CDS |
| TGGAGATGTAAGAGACGCGT+TGG | 0.588827 | 6.2:-1125568 | MS.gene053040:CDS |
| AAAATAAAGGAAGTGTTAAG+GGG | 0.594183 | 6.2:-1125512 | MS.gene053040:CDS |
| GATATAGGTTGCTGGTTGCG+AGG | 0.595916 | 6.2:-1125736 | MS.gene053040:intron |
| GAGTTGATGAGAGTGATGGT+TGG | 0.595956 | 6.2:-1125474 | MS.gene053040:CDS |
| GAGATGTGGTGGAGATGTGG+TGG | 0.610433 | 6.2:-1125592 | MS.gene053040:CDS |
| TTCTGGTGTTGGAGTGCTGG+CGG | 0.616859 | 6.2:-1125688 | MS.gene053040:CDS |
| TGGTTCGAGTACGAGGACGA+GGG | 0.676342 | 6.2:-1125646 | MS.gene053040:CDS |
| TTCAACATAACCATCAACAG+CGG | 0.732160 | 6.2:+1126080 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAGAATTTGCATATATATTA+TGG | + | chr6.2:1125559-1125578 | None:intergenic | 15.0% |
| !! | AAAAAAAAAATGGTCATGTT+TGG | - | chr6.2:1125529-1125548 | MS.gene053040:CDS | 20.0% |
| !! | ATTGCTGATGTTAAAAATAA+AGG | - | chr6.2:1126019-1126038 | MS.gene053040:intron | 20.0% |
| !! | TATTAAGAATTTACTTCCTA+CGG | - | chr6.2:1125492-1125511 | MS.gene053040:CDS | 20.0% |
| !!! | AAAAATAAAGGAAGTGTTAA+GGG | - | chr6.2:1126031-1126050 | MS.gene053040:intron | 20.0% |
| !!! | TAAAAATAAAGGAAGTGTTA+AGG | - | chr6.2:1126030-1126049 | MS.gene053040:intron | 20.0% |
| ! | CTATCTCATTGAATTACTTA+AGG | + | chr6.2:1125727-1125746 | None:intergenic | 25.0% |
| ! | TCTTATCTAATGTAACTGAT+AGG | - | chr6.2:1125765-1125784 | MS.gene053040:intron | 25.0% |
| ! | TTACTTAAATTTAAGTCCGT+AGG | + | chr6.2:1125511-1125530 | None:intergenic | 25.0% |
| !! | AACTTTTACATCTCTGATAT+AGG | - | chr6.2:1125793-1125812 | MS.gene053040:intron | 25.0% |
| !!! | AAAATAAAGGAAGTGTTAAG+GGG | - | chr6.2:1126032-1126051 | MS.gene053040:intron | 25.0% |
| AAAAAAATGGTCATGTTTGG+TGG | - | chr6.2:1125532-1125551 | MS.gene053040:CDS | 30.0% | |
| CTATCTCATTGTATTGCTTA+GGG | + | chr6.2:1125675-1125694 | None:intergenic | 30.0% | |
| GCTCAATCATTCAATTCAAA+TGG | - | chr6.2:1125641-1125660 | MS.gene053040:CDS | 30.0% | |
| TAGCTTGACATGTTTATGAA+TGG | - | chr6.2:1125577-1125596 | MS.gene053040:CDS | 30.0% | |
| !! | TTTAAGAATGTTTCTGGTGT+TGG | - | chr6.2:1125845-1125864 | MS.gene053040:intron | 30.0% |
| AAGCAATACAATGAGATAGC+CGG | - | chr6.2:1125676-1125695 | MS.gene053040:CDS | 35.0% | |
| AGAACAACTAACTCCCAAAT+GGG | - | chr6.2:1125701-1125720 | MS.gene053040:CDS | 35.0% | |
| ATTCAATGAGATAGCCAGTT+AGG | - | chr6.2:1125733-1125752 | MS.gene053040:intron | 35.0% | |
| ATTGTATTGCTTAGGGCTTA+GGG | + | chr6.2:1125668-1125687 | None:intergenic | 35.0% | |
| CAGGAGTTTAAGAATGTTTC+TGG | - | chr6.2:1125839-1125858 | MS.gene053040:intron | 35.0% | |
| GAATTACTTAAGGCCCATTT+GGG | + | chr6.2:1125717-1125736 | None:intergenic | 35.0% | |
| GCTATCTCATTGTATTGCTT+AGG | + | chr6.2:1125676-1125695 | None:intergenic | 35.0% | |
| TAAGAGTTAGTTGTCCTAAC+TGG | + | chr6.2:1125750-1125769 | None:intergenic | 35.0% | |
| TAGAACAACTAACTCCCAAA+TGG | - | chr6.2:1125700-1125719 | MS.gene053040:CDS | 35.0% | |
| TGAATTACTTAAGGCCCATT+TGG | + | chr6.2:1125718-1125737 | None:intergenic | 35.0% | |
| TTCAACATAACCATCAACAG+CGG | + | chr6.2:1125467-1125486 | None:intergenic | 35.0% | |
| TTGCTTAATCTTGTGAGATG+TGG | - | chr6.2:1125938-1125957 | MS.gene053040:intron | 35.0% | |
| !! | GTTGATGGTTATGTTGAATC+AGG | - | chr6.2:1125469-1125488 | MS.gene053040:CDS | 35.0% |
| !!! | TTTTGAGTTGATGAGAGTGA+TGG | - | chr6.2:1126066-1126085 | MS.gene053040:intron | 35.0% |
| AACATCAGCAATTCCATCCA+TGG | + | chr6.2:1126011-1126030 | None:intergenic | 40.0% | |
| AATTTCCAATCCGCTGTTGA+TGG | - | chr6.2:1125454-1125473 | MS.gene053040:CDS | 40.0% | |
| ACATCTCTGATATAGGTTGC+TGG | - | chr6.2:1125800-1125819 | MS.gene053040:intron | 40.0% | |
| CATTGTATTGCTTAGGGCTT+AGG | + | chr6.2:1125669-1125688 | None:intergenic | 40.0% | |
| CTTAATCTTGTGAGATGTGG+TGG | - | chr6.2:1125941-1125960 | MS.gene053040:intron | 40.0% | |
| GATCTACTAGATCTTGCTAC+TGG | - | chr6.2:1125878-1125897 | MS.gene053040:intron | 40.0% | |
| TGGGAGTTAGTTGTTCTAAC+CGG | + | chr6.2:1125698-1125717 | None:intergenic | 40.0% | |
| AAGTGTTAAGGGGCAGAGTA+AGG | - | chr6.2:1126042-1126061 | MS.gene053040:intron | 45.0% | |
| CATAACCATCAACAGCGGAT+TGG | + | chr6.2:1125462-1125481 | None:intergenic | 45.0% | |
| !! | GAGTTGATGAGAGTGATGGT+TGG | - | chr6.2:1126070-1126089 | MS.gene053040:intron | 45.0% |
| !! | GTTGGTGATGTTCGTAGTCA+CGG | - | chr6.2:1126088-1126107 | MS.gene053040:CDS | 45.0% |
| !! | AAATTTAAGTAAAAAAAAAA+TGG | - | chr6.2:1125519-1125538 | MS.gene053040:CDS | 5.0% |
| GACGAGGGAAAATGCAGTGT+CGG | - | chr6.2:1125913-1125932 | MS.gene053040:intron | 50.0% | |
| TGGAGATGTAAGAGACGCGT+TGG | - | chr6.2:1125976-1125995 | MS.gene053040:intron | 50.0% | |
| ! | GATATAGGTTGCTGGTTGCG+AGG | - | chr6.2:1125808-1125827 | MS.gene053040:intron | 50.0% |
| ! | TGTGAGATGTGGTGGAGATG+TGG | - | chr6.2:1125949-1125968 | MS.gene053040:intron | 50.0% |
| !! | TGGTTGCGAGGAAAGTGTTC+AGG | - | chr6.2:1125820-1125839 | MS.gene053040:intron | 50.0% |
| !! | TGTTTCTGGTGTTGGAGTGC+TGG | - | chr6.2:1125853-1125872 | MS.gene053040:intron | 50.0% |
| !! | TTGCTACTGGTTCGAGTACG+AGG | - | chr6.2:1125891-1125910 | MS.gene053040:intron | 50.0% |
| TGGTTCGAGTACGAGGACGA+GGG | - | chr6.2:1125898-1125917 | MS.gene053040:intron | 55.0% | |
| ! | GAGATGTGGTGGAGATGTGG+TGG | - | chr6.2:1125952-1125971 | MS.gene053040:intron | 55.0% |
| ! | GTAAGAGACGCGTTGGCGTT+TGG | - | chr6.2:1125983-1126002 | MS.gene053040:intron | 55.0% |
| !! | TTCTGGTGTTGGAGTGCTGG+CGG | - | chr6.2:1125856-1125875 | MS.gene053040:intron | 55.0% |
| !! | TTGGCGTTTGGTGCCATGGA+TGG | - | chr6.2:1125995-1126014 | MS.gene053040:intron | 55.0% |
| ! | CTGGTTCGAGTACGAGGACG+AGG | - | chr6.2:1125897-1125916 | MS.gene053040:intron | 60.0% |
| ! | TGTGGTGGAGATGTGGTGGC+TGG | - | chr6.2:1125956-1125975 | MS.gene053040:intron | 60.0% |
| !! | CGCGTTGGCGTTTGGTGCCA+TGG | - | chr6.2:1125991-1126010 | MS.gene053040:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 1125446 | 1126120 | 1125446 | ID=MS.gene053040 |
| chr6.2 | mRNA | 1125446 | 1126120 | 1125446 | ID=MS.gene053040.t1;Parent=MS.gene053040 |
| chr6.2 | exon | 1126076 | 1126120 | 1126076 | ID=MS.gene053040.t1.exon1;Parent=MS.gene053040.t1 |
| chr6.2 | CDS | 1126076 | 1126120 | 1126076 | ID=cds.MS.gene053040.t1;Parent=MS.gene053040.t1 |
| chr6.2 | exon | 1125446 | 1125751 | 1125446 | ID=MS.gene053040.t1.exon2;Parent=MS.gene053040.t1 |
| chr6.2 | CDS | 1125446 | 1125751 | 1125446 | ID=cds.MS.gene053040.t1;Parent=MS.gene053040.t1 |
| Gene Sequence |
| Protein sequence |
