AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053152

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053152.t1 MTR_5g080960 70.185 379 106 5 2 376 58 433 0.0 525
MS.gene053152.t1 MTR_8g096990 69.761 377 109 4 2 376 58 431 0.0 524
MS.gene053152.t1 MTR_5g080890 69.657 379 108 5 2 376 58 433 0.0 519
MS.gene053152.t1 MTR_5g044770 69.761 377 108 5 2 376 58 430 0.0 518
MS.gene053152.t1 MTR_8g038280 67.717 381 114 4 2 376 58 435 6.43e-180 507
MS.gene053152.t1 MTR_8g038250 67.277 382 94 5 2 376 58 415 2.44e-176 497
MS.gene053152.t1 MTR_8g038310 59.524 378 133 4 1 378 58 415 4.01e-158 451
MS.gene053152.t1 MTR_6g090290 62.141 383 135 5 1 376 57 436 2.36e-154 442
MS.gene053152.t1 MTR_4g109760 63.492 378 108 5 2 376 48 398 1.11e-152 436
MS.gene053152.t1 MTR_3g028120 54.400 375 163 6 8 376 66 438 3.47e-134 390
MS.gene053152.t1 MTR_2g067830 55.844 385 157 11 1 376 26 406 4.70e-129 377
MS.gene053152.t1 MTR_5g038360 51.538 390 167 10 1 376 55 436 7.78e-123 362
MS.gene053152.t1 MTR_0024s0040 50.773 388 174 9 1 376 28 410 1.53e-116 345
MS.gene053152.t1 MTR_6g088760 53.429 350 148 8 3 341 59 404 1.90e-114 343
MS.gene053152.t1 MTR_2g105300 52.094 382 148 12 1 373 26 381 2.06e-114 338
MS.gene053152.t1 MTR_5g044570 47.906 382 157 11 3 376 64 411 8.49e-108 322
MS.gene053152.t1 MTR_0036s0070 42.368 380 200 10 3 376 60 426 5.81e-96 293
MS.gene053152.t1 MTR_2g059460 45.308 373 186 11 12 376 44 406 2.95e-95 290
MS.gene053152.t1 MTR_0036s0050 43.005 386 198 11 3 376 60 435 4.52e-95 291
MS.gene053152.t1 MTR_5g008070 42.706 377 205 7 6 375 53 425 8.95e-95 290
MS.gene053152.t1 MTR_2g059490 46.761 355 179 8 30 376 58 410 1.47e-94 290
MS.gene053152.t1 MTR_5g008110 42.368 380 201 10 7 376 44 415 7.25e-92 282
MS.gene053152.t1 MTR_0036s0110 40.665 391 206 10 3 376 61 442 2.12e-91 281
MS.gene053152.t1 MTR_5g008150 42.338 385 203 10 3 376 36 412 1.50e-90 278
MS.gene053152.t1 MTR_1g060750 37.760 384 201 12 3 376 21 376 5.11e-74 234
MS.gene053152.t1 MTR_3g097150 37.047 386 225 10 2 376 70 448 1.83e-70 228
MS.gene053152.t1 MTR_4g045857 46.311 244 123 6 18 254 46 288 4.59e-61 199
MS.gene053152.t1 MTR_5g008090 40.134 299 165 9 87 376 21 314 2.00e-60 197
MS.gene053152.t1 MTR_1g115210 35.028 354 210 11 29 374 145 486 1.77e-55 189
MS.gene053152.t1 MTR_7g117430 36.236 356 206 12 29 374 140 484 3.60e-55 189
MS.gene053152.t1 MTR_1g039270 34.366 355 212 11 29 374 127 469 1.29e-54 187
MS.gene053152.t1 MTR_6g088765 43.983 241 102 5 105 344 3 211 1.58e-50 169
MS.gene053152.t1 MTR_1g111350 35.674 356 201 15 29 374 130 467 4.27e-50 175
MS.gene053152.t1 MTR_3g046300 33.938 386 230 13 12 376 79 460 8.55e-47 166
MS.gene053152.t1 MTR_6g085010 33.143 350 215 11 30 374 156 491 1.94e-46 166
MS.gene053152.t1 MTR_2g028190 33.418 395 223 15 9 374 68 451 6.04e-45 160
MS.gene053152.t1 MTR_5g091130 33.161 386 232 13 12 375 173 554 6.11e-44 160
MS.gene053152.t1 MTR_4g007740 32.687 361 212 15 31 375 133 478 1.14e-41 152
MS.gene053152.t1 MTR_3g053710 33.973 365 208 15 29 374 116 466 1.51e-41 152
MS.gene053152.t1 MTR_4g008110 67.544 114 16 1 103 216 45 137 3.80e-41 142
MS.gene053152.t1 MTR_8g103880 30.114 352 225 11 31 374 117 455 2.04e-37 140
MS.gene053152.t1 MTR_7g080230 30.504 377 217 18 29 375 70 431 1.16e-33 130
MS.gene053152.t1 MTR_2g055840 28.460 383 230 13 22 374 5 373 1.41e-33 128
MS.gene053152.t1 MTR_2g070430 31.099 373 215 15 31 371 88 450 2.03e-32 127
MS.gene053152.t1 MTR_3g068000 28.346 381 219 14 33 374 76 441 8.89e-29 117
MS.gene053152.t1 MTR_3g053980 30.874 366 228 15 21 374 133 485 1.38e-28 116
MS.gene053152.t1 MTR_7g105850 28.571 399 214 18 29 376 89 467 1.89e-27 113
MS.gene053152.t1 MTR_7g021510 27.154 383 231 17 26 375 67 434 4.01e-27 112
MS.gene053152.t1 MTR_1g078240 27.047 403 252 17 2 375 41 430 8.58e-27 111
MS.gene053152.t1 MTR_1g078240 27.228 404 251 18 2 375 41 431 1.47e-25 107
MS.gene053152.t1 MTR_3g065160 27.926 376 219 14 31 376 75 428 1.60e-25 107
MS.gene053152.t1 MTR_1g029810 26.738 374 230 14 29 376 56 411 1.71e-25 107
MS.gene053152.t1 MTR_1g029810 26.738 374 230 14 29 376 56 411 1.75e-25 107
MS.gene053152.t1 MTR_5g016260 27.068 399 234 17 3 378 71 435 3.06e-25 107
MS.gene053152.t1 MTR_7g080230 29.513 349 204 18 54 375 28 361 3.93e-25 105
MS.gene053152.t1 MTR_5g016260 27.068 399 234 17 3 378 71 435 4.80e-25 107
MS.gene053152.t1 MTR_6g084710 25.974 385 246 14 20 377 181 553 8.94e-25 106
MS.gene053152.t1 MTR_4g023650 26.667 390 243 14 15 376 47 421 9.27e-25 105
MS.gene053152.t1 MTR_2g084550 28.418 373 231 17 29 375 76 438 9.58e-25 105
MS.gene053152.t1 MTR_8g094590 27.493 371 226 16 29 374 72 424 1.54e-24 104
MS.gene053152.t1 MTR_4g095270 27.068 399 234 17 3 378 63 427 2.90e-24 104
MS.gene053152.t1 MTR_4g095270 27.068 399 234 17 3 378 63 427 4.67e-24 104
MS.gene053152.t1 MTR_7g102750 27.224 371 237 16 29 375 80 441 7.04e-24 103
MS.gene053152.t1 MTR_5g061690 29.630 351 197 19 37 362 108 433 3.13e-23 101
MS.gene053152.t1 MTR_4g023630 25.191 393 245 14 15 376 47 421 6.13e-23 99.8
MS.gene053152.t1 MTR_0945s0010 41.600 125 69 3 253 375 1 123 7.90e-23 94.4
MS.gene053152.t1 MTR_4g056420 28.150 373 231 21 29 375 76 437 2.03e-22 99.0
MS.gene053152.t1 MTR_4g100990 26.859 417 215 18 30 375 74 471 2.12e-22 99.0
MS.gene053152.t1 MTR_1g078240 27.596 337 209 14 2 316 41 364 3.98e-22 97.1
MS.gene053152.t1 MTR_8g094590 28.451 355 210 15 29 362 72 403 8.54e-22 96.3
MS.gene053152.t1 MTR_1g061940 27.346 373 236 17 29 375 82 445 2.25e-21 95.9
MS.gene053152.t1 MTR_5g022880 27.901 362 220 16 36 374 118 461 3.10e-21 95.5
MS.gene053152.t1 MTR_6g084710 26.421 299 193 11 20 308 181 462 4.56e-21 95.1
MS.gene053152.t1 MTR_2g063110 24.685 397 243 16 29 374 117 508 6.63e-21 94.4
MS.gene053152.t1 MTR_5g012490 27.206 408 224 19 30 375 74 470 6.69e-21 94.4
MS.gene053152.t1 MTR_7g062360 27.154 383 219 16 33 374 62 425 8.64e-21 93.6
MS.gene053152.t1 MTR_7g102750 28.662 314 195 14 29 322 80 384 1.16e-20 93.2
MS.gene053152.t1 MTR_8g075090 28.455 369 211 21 36 376 118 461 1.22e-20 94.0
MS.gene053152.t1 MTR_3g014290 29.014 355 224 15 31 375 81 417 2.30e-20 92.4
MS.gene053152.t1 MTR_1g037630 29.014 355 224 15 31 375 93 429 2.95e-20 92.0
MS.gene053152.t1 MTR_8g075090 28.338 367 210 21 36 374 118 459 6.26e-20 91.3
MS.gene053152.t1 MTR_8g039540 28.169 355 228 15 31 375 97 434 8.50e-20 90.9
MS.gene053152.t1 MTR_8g094590 30.078 256 153 9 29 276 72 309 3.19e-19 87.8
MS.gene053152.t1 MTR_3g087780 28.272 382 214 23 27 374 95 450 1.55e-18 87.4
MS.gene053152.t1 MTR_8g075090 29.052 327 188 19 36 338 118 424 3.48e-18 86.3
MS.gene053152.t1 MTR_5g077850 25.594 379 228 15 31 376 82 439 3.97e-18 85.9
MS.gene053152.t1 MTR_5g061690 29.831 295 167 17 84 362 24 294 8.91e-18 84.3
MS.gene053152.t1 MTR_5g080330 26.331 357 196 16 48 374 3 322 7.97e-15 75.9
MS.gene053152.t1 MTR_3g006820 24.041 391 253 17 15 376 39 414 3.53e-14 73.9
MS.gene053152.t1 MTR_3g087780 28.308 325 185 20 73 374 9 308 1.49e-13 71.6
MS.gene053152.t1 MTR_3g087780 28.000 325 186 19 73 374 45 344 3.09e-13 70.9
MS.gene053152.t1 MTR_2g103340 52.542 59 28 0 42 100 67 125 4.35e-13 67.8
MS.gene053152.t1 MTR_3g100500 29.226 349 211 16 29 361 96 424 6.38e-13 70.1
MS.gene053152.t1 MTR_1g072380 24.365 394 217 19 30 369 56 422 2.37e-12 68.6
MS.gene053152.t1 MTR_1g072450 23.762 404 235 19 20 369 30 414 4.94e-12 67.4
MS.gene053152.t1 MTR_2g037710 27.962 211 118 11 175 374 18 205 8.32e-12 64.3
MS.gene053152.t1 MTR_5g077920 23.733 375 242 13 31 376 69 428 8.79e-12 66.6
MS.gene053152.t1 MTR_2g022460 30.000 230 125 13 160 369 171 384 3.51e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053152.t1 AT1G64830 47.656 384 189 7 1 376 51 430 5.62e-112 334
MS.gene053152.t1 AT5G33340 48.031 381 189 8 1 376 60 436 1.53e-108 325
MS.gene053152.t1 AT2G35615 44.757 391 195 9 3 375 57 444 1.85e-99 302
MS.gene053152.t1 AT1G31450 43.990 391 193 10 3 375 60 442 2.27e-90 279
MS.gene053152.t1 AT2G28010 40.209 383 202 11 1 378 35 395 1.32e-84 263
MS.gene053152.t1 AT2G28040 41.514 383 196 12 1 378 35 394 1.04e-81 255
MS.gene053152.t1 AT2G28040 41.514 383 196 11 1 378 26 385 1.54e-81 254
MS.gene053152.t1 AT2G28030 40.274 365 191 11 19 378 49 391 2.39e-76 241
MS.gene053152.t1 AT2G28220 39.378 386 204 11 2 378 37 401 1.13e-75 240
MS.gene053152.t1 AT2G28225 38.108 370 196 11 19 378 55 401 5.20e-71 228
MS.gene053152.t1 AT2G03200 37.990 408 206 15 1 376 68 460 1.50e-65 215
MS.gene053152.t1 AT1G01300 36.872 358 203 13 29 375 140 485 1.42e-55 190
MS.gene053152.t1 AT3G18490 34.565 379 223 14 6 374 137 500 1.42e-53 185
MS.gene053152.t1 AT1G25510 35.294 357 201 13 29 374 146 483 3.44e-51 178
MS.gene053152.t1 AT4G30030 33.676 389 224 16 1 374 50 419 6.36e-51 176
MS.gene053152.t1 AT3G61820 36.088 363 203 13 29 374 133 483 1.10e-49 174
MS.gene053152.t1 AT3G20015 33.148 359 210 13 29 374 129 470 1.14e-49 174
MS.gene053152.t1 AT2G23945 30.874 366 222 12 31 376 96 450 3.62e-46 164
MS.gene053152.t1 AT1G79720 36.685 368 189 17 31 374 135 482 3.57e-43 157
MS.gene053152.t1 AT2G42980 32.902 386 226 16 16 374 145 524 6.57e-42 154
MS.gene053152.t1 AT5G10770 35.734 361 202 16 29 375 130 474 4.32e-41 151
MS.gene053152.t1 AT5G10760 34.565 379 222 14 4 374 104 464 7.23e-41 150
MS.gene053152.t1 AT3G59080 32.208 385 230 17 16 375 155 533 1.83e-40 150
MS.gene053152.t1 AT4G30040 30.914 372 217 17 19 369 75 427 2.76e-40 147
MS.gene053152.t1 AT3G12700 31.781 365 224 13 29 375 104 461 2.44e-39 146
MS.gene053152.t1 AT5G36260 30.133 375 218 16 29 375 76 434 2.94e-39 146
MS.gene053152.t1 AT3G25700 30.788 406 237 17 9 377 54 452 2.77e-38 143
MS.gene053152.t1 AT3G02740 29.640 361 221 16 37 375 16 365 1.26e-32 127
MS.gene053152.t1 AT3G02740 29.268 369 228 16 29 375 83 440 1.30e-32 128
MS.gene053152.t1 AT3G59080 29.737 380 210 15 16 375 155 497 7.10e-31 123
MS.gene053152.t1 AT2G39710 29.737 380 203 18 33 369 67 425 9.52e-31 122
MS.gene053152.t1 AT3G54400 29.534 386 230 16 11 375 61 425 5.91e-30 119
MS.gene053152.t1 AT1G65240 27.968 379 218 17 29 375 72 427 8.95e-28 114
MS.gene053152.t1 AT5G45120 28.434 415 212 21 28 374 81 478 1.55e-27 113
MS.gene053152.t1 AT5G43100 27.692 390 240 16 7 375 50 418 1.04e-26 111
MS.gene053152.t1 AT5G43100 27.692 390 240 16 7 375 50 418 2.98e-26 110
MS.gene053152.t1 AT1G05840 27.614 373 233 15 29 375 78 439 4.32e-26 109
MS.gene053152.t1 AT5G37540 27.733 375 224 16 31 374 80 438 4.99e-26 108
MS.gene053152.t1 AT4G33490 26.065 399 241 16 12 376 35 413 5.99e-26 108
MS.gene053152.t1 AT1G09750 28.536 403 240 18 1 374 65 448 8.82e-26 108
MS.gene053152.t1 AT1G66180 26.842 380 221 16 31 376 72 428 8.87e-26 108
MS.gene053152.t1 AT5G02190 26.203 374 224 16 40 374 82 442 4.29e-24 103
MS.gene053152.t1 AT2G17760 27.805 410 236 22 2 374 63 449 1.16e-23 102
MS.gene053152.t1 AT5G22850 28.610 374 229 18 29 375 79 441 1.40e-23 102
MS.gene053152.t1 AT1G49050 24.513 359 233 15 48 377 49 398 1.52e-23 101
MS.gene053152.t1 AT3G50050 28.198 383 233 15 10 376 82 438 1.54e-23 102
MS.gene053152.t1 AT4G12920 27.324 355 222 13 31 374 58 387 1.73e-23 100
MS.gene053152.t1 AT2G36670 26.287 369 242 14 29 375 98 458 2.97e-23 101
MS.gene053152.t1 AT1G49050 24.138 377 254 14 28 377 200 571 3.16e-23 101
MS.gene053152.t1 AT2G36670 26.593 361 235 14 37 375 111 463 6.64e-23 100
MS.gene053152.t1 AT2G36670 26.316 361 236 14 37 375 40 392 7.42e-23 99.8
MS.gene053152.t1 AT2G36670 26.316 361 236 14 37 375 40 392 7.42e-23 99.8
MS.gene053152.t1 AT1G44130 27.250 400 236 17 11 376 23 401 1.08e-22 99.0
MS.gene053152.t1 AT4G33490 28.618 304 179 13 12 297 32 315 5.07e-22 97.1
MS.gene053152.t1 AT1G08210 26.613 372 236 17 29 375 130 489 6.28e-21 94.7
MS.gene053152.t1 AT1G08210 26.613 372 236 17 29 375 82 441 6.33e-21 94.7
MS.gene053152.t1 AT1G08210 26.613 372 236 17 29 375 116 475 6.56e-21 94.7
MS.gene053152.t1 AT1G77480 26.005 373 235 16 29 376 65 421 4.31e-20 91.7
MS.gene053152.t1 AT3G42550 25.067 371 214 12 21 375 68 390 5.54e-20 91.3
MS.gene053152.t1 AT1G77480 26.005 373 235 16 29 376 65 421 6.12e-20 91.7
MS.gene053152.t1 AT5G07030 27.195 353 211 14 31 362 115 442 2.01e-19 89.7
MS.gene053152.t1 AT3G51350 26.216 370 228 16 31 374 102 452 3.55e-19 89.4
MS.gene053152.t1 AT3G51360 27.424 361 221 16 36 374 10 351 1.20e-17 84.3
MS.gene053152.t1 AT3G51360 27.424 361 221 16 36 374 104 445 1.93e-17 84.0
MS.gene053152.t1 AT3G51360 27.424 361 221 16 36 374 94 435 2.15e-17 84.0
MS.gene053152.t1 AT3G52500 27.525 396 223 23 29 375 88 468 2.42e-17 83.6
MS.gene053152.t1 AT1G08210 27.619 315 195 15 29 322 82 384 7.17e-17 82.4
MS.gene053152.t1 AT3G51360 28.308 325 193 15 36 339 104 409 1.78e-14 75.1
MS.gene053152.t1 AT3G51360 28.308 325 193 15 36 339 94 399 1.82e-14 74.7
MS.gene053152.t1 AT3G51340 24.793 363 226 16 36 374 108 447 2.53e-14 74.7
MS.gene053152.t1 AT5G24820 23.797 395 262 12 9 378 23 403 2.54e-14 74.3
MS.gene053152.t1 AT3G51360 27.458 295 182 11 36 315 94 371 7.38e-14 72.8
MS.gene053152.t1 AT3G51360 27.458 295 182 11 36 315 104 381 8.40e-14 72.8
MS.gene053152.t1 AT5G10080 27.224 371 218 22 37 376 106 455 8.77e-14 73.2
MS.gene053152.t1 AT3G51330 26.503 366 223 17 36 374 107 453 2.40e-13 71.6
MS.gene053152.t1 AT3G25700 31.111 180 106 7 9 171 54 232 2.45e-13 70.9
MS.gene053152.t1 AT3G51330 26.503 366 223 17 36 374 107 453 2.52e-13 71.6
MS.gene053152.t1 AT3G51360 26.733 303 191 14 85 374 25 309 9.41e-13 69.3
MS.gene053152.t1 AT3G51360 26.733 303 191 14 85 374 25 309 9.41e-13 69.3
MS.gene053152.t1 AT3G51340 25.442 283 180 11 36 302 108 375 2.64e-12 68.2

Find 84 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTGGTATTTATGATAATT+TGG 0.139054 4.2:-91833326 MS.gene053152:CDS
GGAGAAGATGCTGTAGTTTC+TGG 0.182846 4.2:-91833520 MS.gene053152:CDS
ACCCCGCCATTTAAGTTATA+TGG 0.209903 4.2:-91834006 MS.gene053152:CDS
TCACTCAAGGAAGGCATCTT+TGG 0.217493 4.2:-91833097 MS.gene053152:CDS
GGAAAGTTCTCTTATTGTTT+GGG 0.226977 4.2:-91833580 MS.gene053152:CDS
TTAAAATGTGCGGTGATTAT+AGG 0.227453 4.2:+91833202 None:intergenic
GGAATCAGCTGTGGTAAAAT+TGG 0.253394 4.2:-91833305 MS.gene053152:CDS
ATCGGGGATCACAGTGGATT+TGG 0.254977 4.2:+91834062 None:intergenic
TGGAAAGTTCTCTTATTGTT+TGG 0.261051 4.2:-91833581 MS.gene053152:CDS
ACAAAACCATATAACTTAAA+TGG 0.265952 4.2:+91834000 None:intergenic
ACAGAATAACAAAGGTTAAA+AGG 0.278658 4.2:+91833247 None:intergenic
AGCGTCTCTTATAAATCAAT+TGG 0.304096 4.2:-91833617 MS.gene053152:CDS
TCATTTCCTAAAACTATGAT+AGG 0.304111 4.2:-91833709 MS.gene053152:CDS
TGCTGATGGGGTTGTTTGTT+TGG 0.319991 4.2:-91833134 MS.gene053152:CDS
TGCAAATATACTATTGAGTA+TGG 0.339141 4.2:-91833805 MS.gene053152:CDS
AATCAATTGGGTTCATCAAT+TGG 0.341653 4.2:-91833604 MS.gene053152:CDS
GGTAACATCATGATTGATTC+AGG 0.346362 4.2:-91833370 MS.gene053152:CDS
CACTCAAGGAAGGCATCTTT+GGG 0.346432 4.2:-91833096 MS.gene053152:CDS
TCGACAAGCAAACTCAGTTT+TGG 0.354009 4.2:-91833541 MS.gene053152:CDS
AACAAAGTCTACACTCAATA+TGG 0.354389 4.2:+91832992 None:intergenic
AGCTCTGGTGTGGTTGGCCT+TGG 0.357134 4.2:-91833649 MS.gene053152:CDS
CACTCAAAGGGTAGTGTTGA+TGG 0.368567 4.2:-91833391 MS.gene053152:CDS
CAAACAACCCCATCAGCAAC+TGG 0.374630 4.2:+91833139 None:intergenic
TAAACGATTCACACGGTTGA+TGG 0.387426 4.2:+91834110 None:intergenic
ACTGGGACAAAGGTGTTAAT+AGG 0.389857 4.2:+91833157 None:intergenic
GGTGTGGTTGGCCTTGGAAA+TGG 0.390490 4.2:-91833643 MS.gene053152:CDS
ATACAGGTAGTGACATAGTT+TGG 0.395461 4.2:-91833975 MS.gene053152:CDS
ATCACATGGAGATCTTAGTT+TGG 0.404282 4.2:-91833770 MS.gene053152:CDS
TCGGGGATCACAGTGGATTT+GGG 0.412343 4.2:+91834063 None:intergenic
CCTTTGTCCCAGTTGCTGAT+GGG 0.431564 4.2:-91833147 MS.gene053152:CDS
ACCTTTGTCCCAGTTGCTGA+TGG 0.433069 4.2:-91833148 MS.gene053152:CDS
GATTAAACATAGGAGTCGTT+TGG 0.435507 4.2:+91833920 None:intergenic
AACCATATAACTTAAATGGC+GGG 0.444894 4.2:+91834004 None:intergenic
GGCCAACCACACCAGAGCTC+TGG 0.446413 4.2:+91833653 None:intergenic
ATAAGAAACTCACCGTCATC+GGG 0.448901 4.2:+91834045 None:intergenic
GGCCAGAGCTCTGGTGTGGT+TGG 0.453163 4.2:-91833655 MS.gene053152:CDS
ATGCCTTCCTTGAGTGAAGA+TGG 0.457005 4.2:+91833103 None:intergenic
TTTAGCTCAACAGAATTTGT+TGG 0.460799 4.2:-91833071 MS.gene053152:CDS
CTTACATTAGATTCCACCTC+TGG 0.468740 4.2:-91833742 MS.gene053152:CDS
TAGTGTTGGAAACAAAATAG+TGG 0.485317 4.2:-91833425 MS.gene053152:CDS
GCGTCTCTTATAAATCAATT+GGG 0.487687 4.2:-91833616 MS.gene053152:CDS
CATAAGAAACTCACCGTCAT+CGG 0.488061 4.2:+91834044 None:intergenic
TCAATATGGTTTGGTACAAT+CGG 0.489521 4.2:+91833006 None:intergenic
TCATTTGAAGGCCAGAGCTC+TGG 0.493916 4.2:-91833664 MS.gene053152:CDS
CCTAAAACTATGATAGGTTG+TGG 0.495262 4.2:-91833703 MS.gene053152:CDS
TGGCGGGGTACCGATTGAAT+AGG 0.499470 4.2:+91834020 None:intergenic
AGTCTACACTCAATATGGTT+TGG 0.504081 4.2:+91832997 None:intergenic
TTTCTTATGACCTATTCAAT+CGG 0.507430 4.2:-91834030 MS.gene053152:CDS
CCCATCAGCAACTGGGACAA+AGG 0.511669 4.2:+91833147 None:intergenic
CTTTGTCCCAGTTGCTGATG+GGG 0.513410 4.2:-91833146 MS.gene053152:CDS
TTGTAACTTGATGATTTCAA+AGG 0.516263 4.2:+91833898 None:intergenic
CTTGTCGAGTTAGACGACTC+TGG 0.532316 4.2:+91833556 None:intergenic
TTCCCATCTTCACTCAAGGA+AGG 0.532641 4.2:-91833106 MS.gene053152:CDS
TATACTATTGAGTATGGTGA+TGG 0.534152 4.2:-91833799 MS.gene053152:CDS
GATGCTGTAGTTTCTGGCCA+TGG 0.536021 4.2:-91833514 MS.gene053152:CDS
ACATTGAAAGCATTTAGTGT+TGG 0.540142 4.2:-91833439 MS.gene053152:CDS
GGAATTTGAAAAGCACTCAA+AGG 0.542170 4.2:-91833404 MS.gene053152:CDS
AGGTTAAAAGGATCATCAGT+TGG 0.545914 4.2:+91833259 None:intergenic
GAATTTGAAAAGCACTCAAA+GGG 0.551524 4.2:-91833403 MS.gene053152:CDS
GCTCAACAGAATTTGTTGGT+TGG 0.553264 4.2:-91833067 MS.gene053152:CDS
GAAAGTTCTCTTATTGTTTG+GGG 0.557774 4.2:-91833579 MS.gene053152:CDS
GATTTCAAAGGATTAAACAT+AGG 0.557880 4.2:+91833910 None:intergenic
ACCATATAACTTAAATGGCG+GGG 0.569617 4.2:+91834005 None:intergenic
TATGGTTTGGTACAATCGGT+AGG 0.569939 4.2:+91833010 None:intergenic
TCCACTGTGATCCCCGATGA+CGG 0.573173 4.2:-91834057 MS.gene053152:CDS
AAACCATATAACTTAAATGG+CGG 0.573940 4.2:+91834003 None:intergenic
TGCCTTCCTTGAGTGAAGAT+GGG 0.574486 4.2:+91833104 None:intergenic
TGATAATTTGGAATCAGCTG+TGG 0.579670 4.2:-91833314 MS.gene053152:CDS
TTGATGTAACAGAATAACAA+AGG 0.586188 4.2:+91833239 None:intergenic
CAATTGGGTTCATCAATTGG+TGG 0.596478 4.2:-91833601 MS.gene053152:CDS
GAGACGCTGGTCCATTTCCA+AGG 0.605463 4.2:+91833632 None:intergenic
TGAAATAGAAGAACCAGAGG+TGG 0.608293 4.2:+91833729 None:intergenic
CTTTGTATAAACGATTCACA+CGG 0.608440 4.2:+91834103 None:intergenic
TGAAGGCCAGAGCTCTGGTG+TGG 0.622062 4.2:-91833659 MS.gene053152:CDS
AAACAACCCCATCAGCAACT+GGG 0.622207 4.2:+91833140 None:intergenic
AAATTATCATAAATACCAGA+TGG 0.626186 4.2:+91833328 None:intergenic
AATTGATTTATAAGAGACGC+TGG 0.633040 4.2:+91833619 None:intergenic
ATCCGCACCTTTAAAATGTG+CGG 0.635428 4.2:+91833192 None:intergenic
TGGTTGTAACAATGTTCACA+AGG 0.636110 4.2:+91833940 None:intergenic
AAGGAGTTGACACAACTCCA+TGG 0.639655 4.2:+91833497 None:intergenic
GGTGATGGATCAATATCACA+TGG 0.658999 4.2:-91833784 MS.gene053152:CDS
AAATGAAATAGAAGAACCAG+AGG 0.674192 4.2:+91833726 None:intergenic
TAAGAAACTCACCGTCATCG+GGG 0.707255 4.2:+91834046 None:intergenic
ACCGTCATCGGGGATCACAG+TGG 0.716584 4.2:+91834056 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATAAAGTAAAAAATTTGT+GGG + chr4.2:91833668-91833687 None:intergenic 10.0%
!! GTTATAAAGTAAAAAATTTG+TGG + chr4.2:91833669-91833688 None:intergenic 15.0%
!! AAATTATCATAAATACCAGA+TGG + chr4.2:91833801-91833820 None:intergenic 20.0%
!! ACAAAACCATATAACTTAAA+TGG + chr4.2:91833129-91833148 None:intergenic 20.0%
!! ATCTGGTATTTATGATAATT+TGG - chr4.2:91833800-91833819 MS.gene053152:CDS 20.0%
!!! AGTTTTTTTTTTACTACAAG+AGG + chr4.2:91833301-91833320 None:intergenic 20.0%
! AAACCATATAACTTAAATGG+CGG + chr4.2:91833126-91833145 None:intergenic 25.0%
! ACAGAATAACAAAGGTTAAA+AGG + chr4.2:91833882-91833901 None:intergenic 25.0%
! GATTTCAAAGGATTAAACAT+AGG + chr4.2:91833219-91833238 None:intergenic 25.0%
! TCATTTCCTAAAACTATGAT+AGG - chr4.2:91833417-91833436 MS.gene053152:CDS 25.0%
! TGCAAATATACTATTGAGTA+TGG - chr4.2:91833321-91833340 MS.gene053152:CDS 25.0%
! TTGATGTAACAGAATAACAA+AGG + chr4.2:91833890-91833909 None:intergenic 25.0%
! TTTCTTATGACCTATTCAAT+CGG - chr4.2:91833096-91833115 MS.gene053152:CDS 25.0%
!! TTGTAACTTGATGATTTCAA+AGG + chr4.2:91833231-91833250 None:intergenic 25.0%
!!! AAAAATAGAGTGTCATTTGA+AGG - chr4.2:91833450-91833469 MS.gene053152:CDS 25.0%
!!! GTACATTTTTTAGACAAACA+AGG + chr4.2:91833264-91833283 None:intergenic 25.0%
!!! TTATATGGTTTTGTTGATAC+AGG - chr4.2:91833135-91833154 MS.gene053152:CDS 25.0%
AAATGAAATAGAAGAACCAG+AGG + chr4.2:91833403-91833422 None:intergenic 30.0%
AACCATATAACTTAAATGGC+GGG + chr4.2:91833125-91833144 None:intergenic 30.0%
AATCAATTGGGTTCATCAAT+TGG - chr4.2:91833522-91833541 MS.gene053152:CDS 30.0%
AGCGTCTCTTATAAATCAAT+TGG - chr4.2:91833509-91833528 MS.gene053152:CDS 30.0%
CTTTGTATAAACGATTCACA+CGG + chr4.2:91833026-91833045 None:intergenic 30.0%
GAAAGTTCTCTTATTGTTTG+GGG - chr4.2:91833547-91833566 MS.gene053152:CDS 30.0%
GCGTCTCTTATAAATCAATT+GGG - chr4.2:91833510-91833529 MS.gene053152:CDS 30.0%
GGAAAGTTCTCTTATTGTTT+GGG - chr4.2:91833546-91833565 MS.gene053152:CDS 30.0%
TATACTATTGAGTATGGTGA+TGG - chr4.2:91833327-91833346 MS.gene053152:CDS 30.0%
TGGAAAGTTCTCTTATTGTT+TGG - chr4.2:91833545-91833564 MS.gene053152:CDS 30.0%
TTAAAATGTGCGGTGATTAT+AGG + chr4.2:91833927-91833946 None:intergenic 30.0%
! AATTGATTTATAAGAGACGC+TGG + chr4.2:91833510-91833529 None:intergenic 30.0%
! ATAATCACCGCACATTTTAA+AGG - chr4.2:91833927-91833946 MS.gene053152:CDS 30.0%
! TAGTGTTGGAAACAAAATAG+TGG - chr4.2:91833701-91833720 MS.gene053152:CDS 30.0%
! TTTAGCTCAACAGAATTTGT+TGG - chr4.2:91834055-91834074 MS.gene053152:CDS 30.0%
!! ACATTGAAAGCATTTAGTGT+TGG - chr4.2:91833687-91833706 MS.gene053152:CDS 30.0%
!! GAATTTGAAAAGCACTCAAA+GGG - chr4.2:91833723-91833742 MS.gene053152:CDS 30.0%
!! TCAATATGGTTTGGTACAAT+CGG + chr4.2:91834123-91834142 None:intergenic 30.0%
ACCATATAACTTAAATGGCG+GGG + chr4.2:91833124-91833143 None:intergenic 35.0%
AGGTTAAAAGGATCATCAGT+TGG + chr4.2:91833870-91833889 None:intergenic 35.0%
ATACAGGTAGTGACATAGTT+TGG - chr4.2:91833151-91833170 MS.gene053152:CDS 35.0%
ATCACATGGAGATCTTAGTT+TGG - chr4.2:91833356-91833375 MS.gene053152:CDS 35.0%
CCTAAAACTATGATAGGTTG+TGG - chr4.2:91833423-91833442 MS.gene053152:CDS 35.0%
GATTAAACATAGGAGTCGTT+TGG + chr4.2:91833209-91833228 None:intergenic 35.0%
TGGTTGTAACAATGTTCACA+AGG + chr4.2:91833189-91833208 None:intergenic 35.0%
! ACAGTGACAATTTTACCATC+TGG - chr4.2:91833783-91833802 MS.gene053152:CDS 35.0%
! CCACAACCTATCATAGTTTT+AGG + chr4.2:91833426-91833445 None:intergenic 35.0%
! GGTAACATCATGATTGATTC+AGG - chr4.2:91833756-91833775 MS.gene053152:CDS 35.0%
! TGATAATTTGGAATCAGCTG+TGG - chr4.2:91833812-91833831 MS.gene053152:CDS 35.0%
!! GGAATTTGAAAAGCACTCAA+AGG - chr4.2:91833722-91833741 MS.gene053152:CDS 35.0%
!!! TGTGGGTCTTTTTTCACTAA+AGG + chr4.2:91833651-91833670 None:intergenic 35.0%
ATAAGAAACTCACCGTCATC+GGG + chr4.2:91833084-91833103 None:intergenic 40.0%
CAATTGGGTTCATCAATTGG+TGG - chr4.2:91833525-91833544 MS.gene053152:CDS 40.0%
CATAAGAAACTCACCGTCAT+CGG + chr4.2:91833085-91833104 None:intergenic 40.0%
CTTACATTAGATTCCACCTC+TGG - chr4.2:91833384-91833403 MS.gene053152:CDS 40.0%
GGAATCAGCTGTGGTAAAAT+TGG - chr4.2:91833821-91833840 MS.gene053152:CDS 40.0%
TAAACGATTCACACGGTTGA+TGG + chr4.2:91833019-91833038 None:intergenic 40.0%
TGAAATAGAAGAACCAGAGG+TGG + chr4.2:91833400-91833419 None:intergenic 40.0%
! ACCCCGCCATTTAAGTTATA+TGG - chr4.2:91833120-91833139 MS.gene053152:CDS 40.0%
! TCGACAAGCAAACTCAGTTT+TGG - chr4.2:91833585-91833604 MS.gene053152:CDS 40.0%
! TCTTTTCCCATCTTCACTCA+AGG - chr4.2:91834016-91834035 MS.gene053152:CDS 40.0%
!! ACTGGGACAAAGGTGTTAAT+AGG + chr4.2:91833972-91833991 None:intergenic 40.0%
!! ATCCGCACCTTTAAAATGTG+CGG + chr4.2:91833937-91833956 None:intergenic 40.0%
!! GCTCAACAGAATTTGTTGGT+TGG - chr4.2:91834059-91834078 MS.gene053152:CDS 40.0%
!! GGTGATGGATCAATATCACA+TGG - chr4.2:91833342-91833361 MS.gene053152:CDS 40.0%
!! TATGGTTTGGTACAATCGGT+AGG + chr4.2:91834119-91834138 None:intergenic 40.0%
AAACAACCCCATCAGCAACT+GGG + chr4.2:91833989-91834008 None:intergenic 45.0%
AAGGAGTTGACACAACTCCA+TGG + chr4.2:91833632-91833651 None:intergenic 45.0%
ATGCCTTCCTTGAGTGAAGA+TGG + chr4.2:91834026-91834045 None:intergenic 45.0%
GGAGAAGATGCTGTAGTTTC+TGG - chr4.2:91833606-91833625 MS.gene053152:CDS 45.0%
TAAGAAACTCACCGTCATCG+GGG + chr4.2:91833083-91833102 None:intergenic 45.0%
TGCCTTCCTTGAGTGAAGAT+GGG + chr4.2:91834025-91834044 None:intergenic 45.0%
TGCTGATGGGGTTGTTTGTT+TGG - chr4.2:91833992-91834011 MS.gene053152:CDS 45.0%
TTCCCATCTTCACTCAAGGA+AGG - chr4.2:91834020-91834039 MS.gene053152:CDS 45.0%
! CACTCAAAGGGTAGTGTTGA+TGG - chr4.2:91833735-91833754 MS.gene053152:CDS 45.0%
!! CACCGCACATTTTAAAGGTG+CGG - chr4.2:91833932-91833951 MS.gene053152:CDS 45.0%
!! CACTCAAGGAAGGCATCTTT+GGG - chr4.2:91834030-91834049 MS.gene053152:CDS 45.0%
!! TCACTCAAGGAAGGCATCTT+TGG - chr4.2:91834029-91834048 MS.gene053152:CDS 45.0%
ACCTTTGTCCCAGTTGCTGA+TGG - chr4.2:91833978-91833997 MS.gene053152:CDS 50.0%
CAAACAACCCCATCAGCAAC+TGG + chr4.2:91833990-91834009 None:intergenic 50.0%
CCTTTGTCCCAGTTGCTGAT+GGG - chr4.2:91833979-91833998 MS.gene053152:CDS 50.0%
CTTGTCGAGTTAGACGACTC+TGG + chr4.2:91833573-91833592 None:intergenic 50.0%
CTTTGTCCCAGTTGCTGATG+GGG - chr4.2:91833980-91833999 MS.gene053152:CDS 50.0%
TCATTTGAAGGCCAGAGCTC+TGG - chr4.2:91833462-91833481 MS.gene053152:CDS 50.0%
! ATCGGGGATCACAGTGGATT+TGG + chr4.2:91833067-91833086 None:intergenic 50.0%
! TCGGGGATCACAGTGGATTT+GGG + chr4.2:91833066-91833085 None:intergenic 50.0%
!! GATGCTGTAGTTTCTGGCCA+TGG - chr4.2:91833612-91833631 MS.gene053152:CDS 50.0%
CCCATCAGCAACTGGGACAA+AGG + chr4.2:91833982-91834001 None:intergenic 55.0%
GAGACGCTGGTCCATTTCCA+AGG + chr4.2:91833497-91833516 None:intergenic 55.0%
TCCACTGTGATCCCCGATGA+CGG - chr4.2:91833069-91833088 MS.gene053152:CDS 55.0%
!! GGTGTGGTTGGCCTTGGAAA+TGG - chr4.2:91833483-91833502 MS.gene053152:CDS 55.0%
!! TGGCGGGGTACCGATTGAAT+AGG + chr4.2:91833109-91833128 None:intergenic 55.0%
ACCGTCATCGGGGATCACAG+TGG + chr4.2:91833073-91833092 None:intergenic 60.0%
! TGAAGGCCAGAGCTCTGGTG+TGG - chr4.2:91833467-91833486 MS.gene053152:CDS 60.0%
!! AGCTCTGGTGTGGTTGGCCT+TGG - chr4.2:91833477-91833496 MS.gene053152:CDS 60.0%
GGCCAACCACACCAGAGCTC+TGG + chr4.2:91833476-91833495 None:intergenic 65.0%
! GGCCAGAGCTCTGGTGTGGT+TGG - chr4.2:91833471-91833490 MS.gene053152:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 91833006 91834142 91833006 ID=MS.gene053152
chr4.2 mRNA 91833006 91834142 91833006 ID=MS.gene053152.t1;Parent=MS.gene053152
chr4.2 exon 91833006 91834142 91833006 ID=MS.gene053152.t1.exon1;Parent=MS.gene053152.t1
chr4.2 CDS 91833006 91834142 91833006 ID=cds.MS.gene053152.t1;Parent=MS.gene053152.t1
Gene Sequence

>MS.gene053152

ATGCATCGTTCCATCAACCGTGTGAATCGTTTATACAAAGATTCTCTTACTAACACACCCAAATCCACTGTGATCCCCGATGACGGTGAGTTTCTTATGACCTATTCAATCGGTACCCCGCCATTTAAGTTATATGGTTTTGTTGATACAGGTAGTGACATAGTTTGGCTTCAATGTAAGCCTTGTGAACATTGTTACAACCAAACGACTCCTATGTTTAATCCTTTGAAATCATCAAGTTACAAAAACAATATTCCTTGTTTGTCTAAAAAATGTACGTCTGTTGAATCTACCTCTTGTAGTAAAAAAAAAACTTGCAAATATACTATTGAGTATGGTGATGGATCAATATCACATGGAGATCTTAGTTTGGAAACTCTTACATTAGATTCCACCTCTGGTTCTTCTATTTCATTTCCTAAAACTATGATAGGTTGTGGACGTAAAAATAGAGTGTCATTTGAAGGCCAGAGCTCTGGTGTGGTTGGCCTTGGAAATGGACCAGCGTCTCTTATAAATCAATTGGGTTCATCAATTGGTGGAAAGTTCTCTTATTGTTTGGGGCCAGAGTCGTCTAACTCGACAAGCAAACTCAGTTTTGGAGAAGATGCTGTAGTTTCTGGCCATGGAGTTGTGTCAACTCCTTTAGTGAAAAAAGACCCACAAATTTTTTACTTTATAACATTGAAAGCATTTAGTGTTGGAAACAAAATAGTGGAATTTGAAAAGCACTCAAAGGGTAGTGTTGATGGTAACATCATGATTGATTCAGGTACAACAGTGACAATTTTACCATCTGGTATTTATGATAATTTGGAATCAGCTGTGGTAAAATTGGTTAAACTGAAGCGTGTAAAAAATCCAACTGATGATCCTTTTAACCTTTGTTATTCTGTTACATCAAATAAATATGATTTTCCTATAATCACCGCACATTTTAAAGGTGCGGATGTTAAGTTGCATCCTATTAACACCTTTGTCCCAGTTGCTGATGGGGTTGTTTGTTTGGCTCTTTTCCCATCTTCACTCAAGGAAGGCATCTTTGGGAATTTAGCTCAACAGAATTTGTTGGTTGGTTATGATCTTCAACAAAAAATCGTGTCTTTTAAGCCTACCGATTGTACCAAACCATATTGA
Protein sequence

>MS.gene053152.t1

MHRSINRVNRLYKDSLTNTPKSTVIPDDGEFLMTYSIGTPPFKLYGFVDTGSDIVWLQCKPCEHCYNQTTPMFNPLKSSSYKNNIPCLSKKCTSVESTSCSKKKTCKYTIEYGDGSISHGDLSLETLTLDSTSGSSISFPKTMIGCGRKNRVSFEGQSSGVVGLGNGPASLINQLGSSIGGKFSYCLGPESSNSTSKLSFGEDAVVSGHGVVSTPLVKKDPQIFYFITLKAFSVGNKIVEFEKHSKGSVDGNIMIDSGTTVTILPSGIYDNLESAVVKLVKLKRVKNPTDDPFNLCYSVTSNKYDFPIITAHFKGADVKLHPINTFVPVADGVVCLALFPSSLKEGIFGNLAQQNLLVGYDLQQKIVSFKPTDCTKPY