AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053153

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053153.t1 MTR_8g096990 66.897 435 138 5 1 433 1 431 0.0 583
MS.gene053153.t1 MTR_5g080960 66.362 437 139 6 1 433 1 433 0.0 569
MS.gene053153.t1 MTR_5g080890 65.904 437 141 6 1 433 1 433 0.0 563
MS.gene053153.t1 MTR_8g038280 65.668 434 139 5 6 433 6 435 0.0 562
MS.gene053153.t1 MTR_5g044770 66.279 430 138 6 6 433 6 430 0.0 559
MS.gene053153.t1 MTR_8g038250 65.000 440 122 6 1 433 1 415 0.0 554
MS.gene053153.t1 MTR_8g038310 59.540 435 156 4 1 435 1 415 0.0 526
MS.gene053153.t1 MTR_6g090290 61.451 441 157 7 1 433 1 436 8.35e-175 496
MS.gene053153.t1 MTR_4g109760 57.569 436 144 8 1 433 1 398 3.69e-152 437
MS.gene053153.t1 MTR_3g028120 51.566 415 190 7 28 433 26 438 8.38e-140 407
MS.gene053153.t1 MTR_2g067830 54.634 410 173 11 33 433 1 406 4.11e-136 397
MS.gene053153.t1 MTR_6g088760 52.300 413 181 9 1 402 1 408 2.49e-131 389
MS.gene053153.t1 MTR_5g038360 48.165 436 208 11 9 433 8 436 2.73e-128 378
MS.gene053153.t1 MTR_2g105300 51.351 407 163 12 33 430 1 381 6.63e-122 360
MS.gene053153.t1 MTR_0024s0040 47.596 416 199 10 31 433 1 410 1.70e-117 349
MS.gene053153.t1 MTR_5g044570 43.341 443 207 11 3 433 1 411 3.45e-112 336
MS.gene053153.t1 MTR_5g008070 41.388 418 223 10 22 432 23 425 2.19e-98 301
MS.gene053153.t1 MTR_0036s0050 41.050 419 225 11 27 433 27 435 4.62e-98 300
MS.gene053153.t1 MTR_0036s0070 40.000 435 234 12 11 433 7 426 8.35e-98 300
MS.gene053153.t1 MTR_0036s0110 39.207 454 243 14 1 433 1 442 3.64e-97 298
MS.gene053153.t1 MTR_2g059490 41.419 437 212 13 5 432 8 409 5.07e-95 293
MS.gene053153.t1 MTR_2g059460 41.388 418 201 13 24 433 25 406 5.88e-94 289
MS.gene053153.t1 MTR_5g008110 36.782 435 231 9 6 433 18 415 2.18e-87 272
MS.gene053153.t1 MTR_5g008150 38.810 420 214 12 23 433 27 412 2.47e-85 267
MS.gene053153.t1 MTR_1g060750 34.739 403 225 12 41 433 2 376 2.85e-68 222
MS.gene053153.t1 MTR_3g097150 33.810 420 251 11 28 433 42 448 9.45e-66 217
MS.gene053153.t1 MTR_5g008090 39.000 300 167 10 144 433 21 314 5.08e-58 193
MS.gene053153.t1 MTR_4g045857 38.994 318 155 10 3 311 1 288 1.80e-57 191
MS.gene053153.t1 MTR_7g117430 35.294 357 208 13 86 431 140 484 3.50e-52 182
MS.gene053153.t1 MTR_1g115210 31.002 429 270 11 18 431 69 486 2.15e-51 180
MS.gene053153.t1 MTR_1g039270 33.521 355 215 11 86 431 127 469 1.67e-50 177
MS.gene053153.t1 MTR_6g088765 43.277 238 102 5 162 398 3 208 4.29e-49 167
MS.gene053153.t1 MTR_4g008110 77.876 113 4 1 161 273 46 137 1.34e-48 162
MS.gene053153.t1 MTR_1g111350 34.746 354 207 13 86 431 130 467 3.23e-47 169
MS.gene053153.t1 MTR_6g085010 32.571 350 217 10 87 431 156 491 2.44e-40 150
MS.gene053153.t1 MTR_2g028190 32.432 370 223 14 78 431 93 451 2.63e-39 147
MS.gene053153.t1 MTR_3g046300 32.821 390 229 14 69 433 79 460 3.40e-38 144
MS.gene053153.t1 MTR_3g053710 33.609 363 212 15 86 431 116 466 1.88e-37 142
MS.gene053153.t1 MTR_4g007740 31.014 345 209 14 104 432 147 478 4.93e-36 138
MS.gene053153.t1 MTR_5g091130 30.848 389 237 15 69 432 173 554 1.17e-34 135
MS.gene053153.t1 MTR_8g103880 30.263 380 230 16 67 431 96 455 2.71e-32 127
MS.gene053153.t1 MTR_3g068000 29.101 378 220 15 90 431 76 441 6.71e-31 124
MS.gene053153.t1 MTR_2g070430 28.788 462 274 17 6 428 5 450 4.86e-30 121
MS.gene053153.t1 MTR_7g080230 27.002 437 268 19 31 432 11 431 6.68e-29 118
MS.gene053153.t1 MTR_7g105850 30.044 456 234 24 41 433 34 467 2.68e-27 114
MS.gene053153.t1 MTR_3g065160 27.807 374 222 16 88 433 75 428 1.44e-26 111
MS.gene053153.t1 MTR_2g055840 26.720 378 243 13 79 431 5 373 4.54e-25 106
MS.gene053153.t1 MTR_0945s0010 40.800 125 70 4 310 432 1 123 1.48e-24 99.8
MS.gene053153.t1 MTR_7g021510 27.225 382 225 18 86 432 71 434 1.83e-24 105
MS.gene053153.t1 MTR_3g053980 29.315 365 235 13 78 431 133 485 3.46e-24 105
MS.gene053153.t1 MTR_7g062360 26.984 378 226 15 90 431 62 425 7.71e-24 103
MS.gene053153.t1 MTR_8g094590 25.876 371 232 14 86 431 72 424 1.22e-22 100
MS.gene053153.t1 MTR_5g077850 25.995 377 229 15 88 433 82 439 1.90e-22 99.4
MS.gene053153.t1 MTR_1g078240 26.355 406 250 20 62 432 39 430 2.58e-22 99.4
MS.gene053153.t1 MTR_1g078240 26.961 408 246 21 62 432 39 431 1.39e-21 97.1
MS.gene053153.t1 MTR_4g095270 25.424 413 263 17 42 433 37 425 1.61e-21 97.8
MS.gene053153.t1 MTR_4g095270 25.424 413 263 17 42 433 37 425 1.67e-21 97.4
MS.gene053153.t1 MTR_5g016260 25.405 370 231 16 86 433 87 433 1.91e-21 97.4
MS.gene053153.t1 MTR_5g016260 25.405 370 231 16 86 433 87 433 2.01e-21 97.4
MS.gene053153.t1 MTR_2g103340 42.735 117 23 1 21 137 33 105 2.17e-21 91.7
MS.gene053153.t1 MTR_1g029810 26.064 376 230 16 86 433 56 411 3.22e-21 95.5
MS.gene053153.t1 MTR_1g029810 26.064 376 230 16 86 433 56 411 3.74e-21 95.5
MS.gene053153.t1 MTR_3g096930 24.742 388 238 16 79 433 70 436 5.78e-21 95.1
MS.gene053153.t1 MTR_6g084710 26.385 379 234 15 86 434 190 553 1.06e-20 95.1
MS.gene053153.t1 MTR_4g056420 27.926 376 228 18 86 432 76 437 1.35e-20 94.4
MS.gene053153.t1 MTR_7g080230 27.762 353 208 17 111 432 25 361 2.29e-20 92.8
MS.gene053153.t1 MTR_8g094590 28.777 278 171 10 86 354 72 331 3.18e-20 92.4
MS.gene053153.t1 MTR_4g023650 25.189 397 250 16 67 433 42 421 3.73e-20 92.4
MS.gene053153.t1 MTR_1g037630 28.235 425 264 17 27 432 27 429 4.80e-20 92.4
MS.gene053153.t1 MTR_2g084550 29.178 377 223 18 86 432 76 438 5.58e-20 92.4
MS.gene053153.t1 MTR_8g094590 29.297 256 155 9 86 333 72 309 1.13e-19 89.7
MS.gene053153.t1 MTR_4g100990 27.603 413 215 20 87 431 74 470 2.08e-19 90.9
MS.gene053153.t1 MTR_3g014290 29.050 358 220 15 88 432 81 417 6.27e-19 89.0
MS.gene053153.t1 MTR_5g012490 26.536 407 226 19 87 431 74 469 1.16e-18 88.6
MS.gene053153.t1 MTR_1g078240 27.647 340 204 18 62 373 39 364 1.43e-18 87.4
MS.gene053153.t1 MTR_4g023630 25.189 397 250 16 67 433 42 421 1.81e-18 87.4
MS.gene053153.t1 MTR_6g084710 26.625 323 205 13 86 397 190 491 3.59e-18 87.0
MS.gene053153.t1 MTR_5g077920 25.197 381 229 14 88 433 69 428 4.69e-18 86.3
MS.gene053153.t1 MTR_1g061940 26.203 374 239 18 86 432 82 445 3.21e-17 84.0
MS.gene053153.t1 MTR_5g080330 26.816 358 195 17 104 431 2 322 3.78e-17 83.6
MS.gene053153.t1 MTR_8g039540 28.795 448 262 24 16 432 13 434 4.91e-17 83.2
MS.gene053153.t1 MTR_7g102750 25.269 372 243 16 86 432 80 441 3.41e-16 80.9
MS.gene053153.t1 MTR_2g063110 23.333 480 275 20 29 431 45 508 1.97e-15 78.6
MS.gene053153.t1 MTR_7g102750 26.623 308 196 14 86 373 80 377 8.06e-14 73.6
MS.gene053153.t1 MTR_3g087780 24.070 457 260 23 29 431 27 450 1.96e-13 72.4
MS.gene053153.t1 MTR_3g100500 27.293 447 263 20 7 418 5 424 3.59e-13 71.2
MS.gene053153.t1 MTR_8g075090 26.087 368 221 20 93 433 118 461 4.66e-13 71.2
MS.gene053153.t1 MTR_8g075090 25.956 366 220 20 93 431 118 459 2.04e-12 69.3
MS.gene053153.t1 MTR_3g006820 22.762 391 258 15 72 433 39 414 1.23e-11 66.6
MS.gene053153.t1 MTR_5g061690 26.621 293 179 15 141 419 24 294 4.97e-11 64.3
MS.gene053153.t1 MTR_8g075090 26.490 302 183 16 93 374 118 400 9.90e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053153.t1 AT1G64830 45.742 411 215 7 27 433 24 430 1.09e-121 361
MS.gene053153.t1 AT5G33340 48.175 411 204 8 28 433 30 436 3.08e-121 360
MS.gene053153.t1 AT2G35615 43.991 441 219 12 16 432 8 444 3.17e-107 324
MS.gene053153.t1 AT1G31450 41.071 448 242 10 1 432 1 442 3.87e-97 298
MS.gene053153.t1 AT2G28010 39.679 436 206 16 6 435 11 395 1.66e-85 267
MS.gene053153.t1 AT2G28040 39.030 433 202 16 9 432 12 391 9.32e-81 254
MS.gene053153.t1 AT2G28040 39.759 415 190 15 27 432 19 382 9.50e-79 249
MS.gene053153.t1 AT2G28030 43.750 352 169 13 88 433 61 389 3.21e-77 245
MS.gene053153.t1 AT2G28220 37.241 435 220 15 7 432 8 398 2.31e-75 241
MS.gene053153.t1 AT2G28225 36.009 436 226 15 7 433 8 399 3.35e-68 222
MS.gene053153.t1 AT2G03200 35.308 439 239 16 23 433 39 460 1.41e-59 201
MS.gene053153.t1 AT1G01300 36.695 357 203 13 86 431 140 484 7.45e-54 187
MS.gene053153.t1 AT3G61820 36.088 363 203 13 86 431 133 483 1.24e-48 173
MS.gene053153.t1 AT3G18490 30.872 447 261 17 7 431 80 500 1.61e-48 173
MS.gene053153.t1 AT3G20015 30.365 438 260 16 21 431 51 470 1.74e-47 169
MS.gene053153.t1 AT1G25510 33.239 352 215 10 86 431 146 483 9.21e-45 162
MS.gene053153.t1 AT4G30030 32.418 364 220 15 80 431 70 419 1.20e-42 155
MS.gene053153.t1 AT2G23945 27.623 467 287 15 1 433 1 450 1.26e-42 156
MS.gene053153.t1 AT5G10770 35.467 375 207 16 78 432 115 474 5.24e-40 149
MS.gene053153.t1 AT5G10760 31.981 419 250 16 30 431 64 464 6.11e-40 149
MS.gene053153.t1 AT4G30040 30.649 447 256 24 5 426 10 427 1.88e-36 139
MS.gene053153.t1 AT3G12700 28.442 443 273 17 17 432 36 461 2.13e-36 139
MS.gene053153.t1 AT1G79720 34.232 371 194 19 88 431 135 482 4.25e-36 139
MS.gene053153.t1 AT3G25700 30.423 378 227 16 86 434 82 452 2.25e-35 136
MS.gene053153.t1 AT2G42980 30.366 382 241 13 73 431 145 524 1.26e-32 129
MS.gene053153.t1 AT2G39710 29.947 374 210 17 90 426 67 425 1.36e-32 128
MS.gene053153.t1 AT5G36260 27.513 378 224 16 86 432 76 434 8.94e-32 127
MS.gene053153.t1 AT3G59080 29.474 380 245 14 73 431 155 532 4.77e-31 125
MS.gene053153.t1 AT5G02190 27.151 372 223 16 97 431 82 442 1.73e-28 117
MS.gene053153.t1 AT1G66180 25.397 378 229 15 88 433 72 428 8.47e-26 108
MS.gene053153.t1 AT1G09750 28.070 399 245 15 58 431 67 448 2.19e-25 108
MS.gene053153.t1 AT5G37540 27.200 375 226 16 88 431 80 438 7.53e-25 106
MS.gene053153.t1 AT3G02740 26.902 368 238 16 86 432 83 440 1.33e-24 106
MS.gene053153.t1 AT3G02740 27.222 360 231 16 94 432 16 365 1.98e-24 105
MS.gene053153.t1 AT3G54400 28.974 390 237 17 63 432 56 425 3.65e-24 104
MS.gene053153.t1 AT1G65240 25.238 420 257 17 50 432 28 427 4.07e-24 104
MS.gene053153.t1 AT3G59080 27.200 375 224 12 73 431 155 496 6.83e-24 104
MS.gene053153.t1 AT5G43100 27.248 367 229 14 84 432 72 418 3.99e-23 102
MS.gene053153.t1 AT5G43100 27.248 367 229 14 84 432 72 418 8.56e-23 101
MS.gene053153.t1 AT5G45120 27.295 414 213 21 88 431 83 478 1.63e-22 100
MS.gene053153.t1 AT4G33490 25.935 401 239 18 69 433 35 413 4.50e-22 98.2
MS.gene053153.t1 AT1G44130 26.817 399 237 18 69 433 24 401 8.91e-22 97.1
MS.gene053153.t1 AT5G22850 28.042 378 226 18 86 432 79 441 6.93e-21 95.1
MS.gene053153.t1 AT2G17760 27.198 364 217 18 93 431 109 449 2.03e-20 94.0
MS.gene053153.t1 AT1G49050 23.876 356 239 12 105 434 49 398 7.04e-20 91.7
MS.gene053153.t1 AT1G05840 25.789 380 231 15 86 432 78 439 1.68e-19 90.9
MS.gene053153.t1 AT1G49050 23.876 356 239 12 105 434 222 571 2.38e-19 90.9
MS.gene053153.t1 AT4G33490 27.703 296 174 13 69 344 32 307 1.11e-18 87.8
MS.gene053153.t1 AT3G50050 25.341 367 236 14 86 433 91 438 1.25e-18 89.0
MS.gene053153.t1 AT2G36670 26.486 370 240 17 86 432 98 458 3.48e-18 87.0
MS.gene053153.t1 AT5G07030 28.153 444 265 21 7 419 22 442 3.92e-18 86.7
MS.gene053153.t1 AT1G08210 26.471 374 234 17 86 432 130 489 5.04e-18 86.7
MS.gene053153.t1 AT1G08210 26.471 374 234 17 86 432 116 475 5.27e-18 86.7
MS.gene053153.t1 AT1G08210 26.596 376 231 17 86 432 82 441 5.71e-18 86.3
MS.gene053153.t1 AT2G36670 26.796 362 233 17 94 432 111 463 6.42e-18 86.3
MS.gene053153.t1 AT2G36670 26.796 362 233 17 94 432 40 392 6.64e-18 85.9
MS.gene053153.t1 AT2G36670 26.796 362 233 17 94 432 40 392 6.64e-18 85.9
MS.gene053153.t1 AT3G51350 26.630 368 219 19 93 431 107 452 4.75e-17 83.6
MS.gene053153.t1 AT3G42550 22.992 361 214 12 88 432 78 390 1.22e-16 82.0
MS.gene053153.t1 AT1G49050 26.800 250 161 9 105 342 222 461 4.97e-16 80.5
MS.gene053153.t1 AT1G08210 24.814 403 231 18 86 432 82 468 2.78e-14 75.1
MS.gene053153.t1 AT3G51340 25.414 362 225 18 93 431 108 447 6.38e-13 70.9
MS.gene053153.t1 AT5G24820 26.122 245 161 8 200 435 170 403 2.23e-12 68.9
MS.gene053153.t1 AT3G25700 30.709 127 79 4 86 204 82 207 4.29e-12 67.8
MS.gene053153.t1 AT3G51360 23.923 418 269 15 12 399 14 412 4.06e-11 65.1
MS.gene053153.t1 AT3G51360 23.923 418 269 15 12 399 4 402 5.44e-11 64.7
MS.gene053153.t1 AT3G51340 23.861 373 229 18 93 431 108 459 6.70e-11 64.7

Find 81 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAAATGTGCAGTGATTA+TGG 0.131217 4.2:+91899811 None:intergenic
ATCTGGTATTTATCATAATT+TGG 0.150549 4.2:-91899936 MS.gene053153:CDS
GGAGAAGCTGCTATAGTTTC+TGG 0.180325 4.2:-91900130 MS.gene053153:CDS
TCAACGAGCAAACTCAATTT+TGG 0.205188 4.2:-91900151 MS.gene053153:CDS
GGAAAGTTCTCTTATTGTTT+GGG 0.226977 4.2:-91900190 MS.gene053153:CDS
AGCGGGCATCACAGTGGATT+TGG 0.236368 4.2:+91900672 None:intergenic
ACCCCACCATTTAAGTTATA+TGG 0.251212 4.2:-91900616 MS.gene053153:CDS
CCTAAAACCGTGATAGGTTT+TGG 0.252734 4.2:-91900313 MS.gene053153:CDS
CTACTCAATAAAGGCATCTT+TGG 0.257937 4.2:-91899707 MS.gene053153:CDS
TGGAAAGTTCTCTTATTGTT+TGG 0.261051 4.2:-91900191 MS.gene053153:CDS
ACAATACCATATAACTTAAA+TGG 0.274831 4.2:+91900610 None:intergenic
CGTGTCTCTTATAAATCAAT+TGG 0.312361 4.2:-91900227 MS.gene053153:CDS
TCATTTCCTAAAACCGTGAT+AGG 0.315869 4.2:-91900319 MS.gene053153:CDS
AATCAATTGGGTTCATCAAT+TGG 0.341653 4.2:-91900214 MS.gene053153:CDS
TGGATTTGGGTGTGTTAGTA+AGG 0.346495 4.2:+91900686 None:intergenic
AACAAAGTCTACACTCAATA+TGG 0.354389 4.2:+91899602 None:intergenic
GGTAACATCATAGTTGATTC+AGG 0.362416 4.2:-91899980 MS.gene053153:CDS
GCGGGCATCACAGTGGATTT+GGG 0.367396 4.2:+91900673 None:intergenic
GTCCGATCTCTACTCAATAA+AGG 0.384414 4.2:-91899716 MS.gene053153:CDS
GGTGTGGTTGGCCTTGGAAA+TGG 0.390490 4.2:-91900253 MS.gene053153:CDS
ACCTTTGCTCGAGTTTCTGA+TGG 0.391081 4.2:-91899758 MS.gene053153:CDS
TGCGAATATAGTATTAAGTA+TGG 0.394209 4.2:-91900415 MS.gene053153:CDS
TCAAAATGTGCAGTGATTAT+GGG 0.395459 4.2:+91899812 None:intergenic
CAATCAAATGGTAGTGTTGA+TGG 0.396941 4.2:-91900001 MS.gene053153:CDS
AGCTCTGGTGTGGTTGGCCT+TGG 0.398793 4.2:-91900259 MS.gene053153:CDS
AACAATACATTGTCATTTGA+AGG 0.406579 4.2:-91900286 MS.gene053153:CDS
TACTCAATAAAGGCATCTTT+GGG 0.415339 4.2:-91899706 MS.gene053153:CDS
ATCACAAGGAGATCTTAGTT+TGG 0.415779 4.2:-91900380 MS.gene053153:CDS
ATCATATTTATTTGATGCAA+CGG 0.425117 4.2:+91899838 None:intergenic
TCTCTTATTGTTTGGGGCCA+TGG 0.430440 4.2:-91900183 MS.gene053153:CDS
CATAAGAAACTCACCTTTAG+CGG 0.438405 4.2:+91900654 None:intergenic
TTATATGGTATTGTTGATAC+AGG 0.443331 4.2:-91900601 MS.gene053153:CDS
ATACAGGTAGTGACATGGTT+TGG 0.456250 4.2:-91900585 MS.gene053153:CDS
CCTTTGCTCGAGTTTCTGAT+GGG 0.459517 4.2:-91899757 MS.gene053153:CDS
TTTAGCTCAACAGAATTTGT+TGG 0.460799 4.2:-91899681 MS.gene053153:CDS
CTTACATTAGATTCCACCTC+TGG 0.468740 4.2:-91900352 MS.gene053153:CDS
TCATTTGAAGGCAAGAGCTC+TGG 0.470790 4.2:-91900274 MS.gene053153:CDS
TGGTGGGGTACCGACTGAAT+AGG 0.480047 4.2:+91900630 None:intergenic
GGCAAGAGCTCTGGTGTGGT+TGG 0.491732 4.2:-91900265 MS.gene053153:CDS
TACCATATAACTTAAATGGT+GGG 0.492301 4.2:+91900614 None:intergenic
GTGTCTCTTATAAATCAATT+GGG 0.493038 4.2:-91900226 MS.gene053153:CDS
CTTTCTAATGCTCTAAGCAA+TGG 0.494056 4.2:-91900841 MS.gene053153:CDS
TCCACTGTGATGCCCGCTAA+AGG 0.499859 4.2:-91900667 MS.gene053153:CDS
AGTCTACACTCAATATGGTT+TGG 0.504081 4.2:+91899607 None:intergenic
TTGTAACTTGATGAATTCAA+AGG 0.507205 4.2:+91900508 None:intergenic
AAGCGTGCAAAGGATCCAAC+TGG 0.524991 4.2:-91899884 MS.gene053153:CDS
TATAGTATTAAGTATGGTGA+TGG 0.532681 4.2:-91900409 MS.gene053153:CDS
GCAGTGACAGTTGTACCATC+TGG 0.537641 4.2:-91899953 MS.gene053153:CDS
ACATTGAAAGCATTTAGTGT+TGG 0.540142 4.2:-91900049 MS.gene053153:CDS
GCTGCTATAGTTTCTGGTCA+TGG 0.541311 4.2:-91900124 MS.gene053153:CDS
TGCCTTTATTGAGTAGAGAT+CGG 0.542952 4.2:+91899714 None:intergenic
TCATAATTTGGTATCAGCTG+TGG 0.549938 4.2:-91899924 MS.gene053153:CDS
GCTCAACAGAATTTGTTGGT+TGG 0.553264 4.2:-91899677 MS.gene053153:CDS
GAAAGTTCTCTTATTGTTTG+GGG 0.557774 4.2:-91900189 MS.gene053153:CDS
GTTATGTCCAAAACCTATCA+CGG 0.558819 4.2:+91900306 None:intergenic
TATGGTTTGGTACAATCAGT+AGG 0.559170 4.2:+91899620 None:intergenic
ATACCATATAACTTAAATGG+TGG 0.565852 4.2:+91900613 None:intergenic
CTCGTTGAGTTAGACGACCA+TGG 0.566118 4.2:+91900166 None:intergenic
AGATTAAGAAAACCACCAGT+TGG 0.573212 4.2:+91899869 None:intergenic
GGATTTGGGTGTGTTAGTAA+GGG 0.573804 4.2:+91900687 None:intergenic
ACCATATAACTTAAATGGTG+GGG 0.576438 4.2:+91900615 None:intergenic
ATAAGAAACTCACCTTTAGC+GGG 0.577860 4.2:+91900655 None:intergenic
CAATTGATTTATAAGAGACA+CGG 0.582532 4.2:+91900228 None:intergenic
TGGTTGTAACAGTGTTCACA+AGG 0.587638 4.2:+91900550 None:intergenic
CCCATCAGAAACTCGAGCAA+AGG 0.593408 4.2:+91899757 None:intergenic
CAATTGGGTTCATCAATTGG+TGG 0.596478 4.2:-91900211 MS.gene053153:CDS
GAGACACGGGTCCATTTCCA+AGG 0.606926 4.2:+91900242 None:intergenic
CTTTGCTCGAGTTTCTGATG+GGG 0.607379 4.2:-91899756 MS.gene053153:CDS
GTAATGATCGACATGATCGA+TGG 0.608118 4.2:+91900720 None:intergenic
TGAAATAGAAGAACCAGAGG+TGG 0.608293 4.2:+91900339 None:intergenic
AGTTAAACTGAAGCGTGCAA+AGG 0.614291 4.2:-91899894 MS.gene053153:CDS
TTTCTTATGACCTATTCAGT+CGG 0.625475 4.2:-91900640 MS.gene053153:CDS
TGTTGATACAGGTAGTGACA+TGG 0.630223 4.2:-91900590 MS.gene053153:CDS
AATTGATTTATAAGAGACAC+GGG 0.646628 4.2:+91900229 None:intergenic
AAATTATGATAAATACCAGA+TGG 0.652242 4.2:+91899938 None:intergenic
TGAAGGCAAGAGCTCTGGTG+TGG 0.663721 4.2:-91900269 MS.gene053153:CDS
ACCTTTAGCGGGCATCACAG+TGG 0.667290 4.2:+91900666 None:intergenic
AAATGAAATAGAAGAACCAG+AGG 0.674192 4.2:+91900336 None:intergenic
CGTGCAAAGGATCCAACTGG+TGG 0.682034 4.2:-91899881 MS.gene053153:CDS
GGTGATGGATCAATATCACA+AGG 0.695175 4.2:-91900394 MS.gene053153:CDS
GCATAGCATTGACAACACGT+TGG 0.726271 4.2:+91900749 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTAAGTAAGCAATTTTT+GGG + chr4.2:91900449-91900468 None:intergenic 15.0%
!! AAATTATGATAAATACCAGA+TGG + chr4.2:91900582-91900601 None:intergenic 20.0%
!! ACAATACCATATAACTTAAA+TGG + chr4.2:91899910-91899929 None:intergenic 20.0%
!! ATCATATTTATTTGATGCAA+CGG + chr4.2:91900682-91900701 None:intergenic 20.0%
!! ATCTGGTATTTATCATAATT+TGG - chr4.2:91900581-91900600 MS.gene053153:CDS 20.0%
!! GAATTCAAAGGATTAAAAAT+AGG + chr4.2:91900000-91900019 None:intergenic 20.0%
!!! AGTTTTGTTTTTATTACAAG+AGG + chr4.2:91900082-91900101 None:intergenic 20.0%
!!! GTTATTAAGTAAGCAATTTT+TGG + chr4.2:91900450-91900469 None:intergenic 20.0%
!!! TTACATTTTTTAGACAAACA+AGG + chr4.2:91900045-91900064 None:intergenic 20.0%
! ATACCATATAACTTAAATGG+TGG + chr4.2:91899907-91899926 None:intergenic 25.0%
! GTGTCTCTTATAAATCAATT+GGG - chr4.2:91900291-91900310 MS.gene053153:CDS 25.0%
! TACCATATAACTTAAATGGT+GGG + chr4.2:91899906-91899925 None:intergenic 25.0%
! TATAGTATTAAGTATGGTGA+TGG - chr4.2:91900108-91900127 MS.gene053153:CDS 25.0%
! TGCGAATATAGTATTAAGTA+TGG - chr4.2:91900102-91900121 MS.gene053153:CDS 25.0%
! TTATATGGTATTGTTGATAC+AGG - chr4.2:91899916-91899935 MS.gene053153:CDS 25.0%
! TTGTAACTTGATGAATTCAA+AGG + chr4.2:91900012-91900031 None:intergenic 25.0%
!! AACAATACATTGTCATTTGA+AGG - chr4.2:91900231-91900250 MS.gene053153:CDS 25.0%
!! AATTGATTTATAAGAGACAC+GGG + chr4.2:91900291-91900310 None:intergenic 25.0%
!! CAATTGATTTATAAGAGACA+CGG + chr4.2:91900292-91900311 None:intergenic 25.0%
!! GAATTTTCAAAGCAATCAAA+TGG - chr4.2:91900504-91900523 MS.gene053153:CDS 25.0%
AAATGAAATAGAAGAACCAG+AGG + chr4.2:91900184-91900203 None:intergenic 30.0%
AATCAATTGGGTTCATCAAT+TGG - chr4.2:91900303-91900322 MS.gene053153:CDS 30.0%
ACCATATAACTTAAATGGTG+GGG + chr4.2:91899905-91899924 None:intergenic 30.0%
CGTGTCTCTTATAAATCAAT+TGG - chr4.2:91900290-91900309 MS.gene053153:CDS 30.0%
GAAAGTTCTCTTATTGTTTG+GGG - chr4.2:91900328-91900347 MS.gene053153:CDS 30.0%
GATTAAAAATAGGAGTCGTT+TGG + chr4.2:91899990-91900009 None:intergenic 30.0%
GGAAAGTTCTCTTATTGTTT+GGG - chr4.2:91900327-91900346 MS.gene053153:CDS 30.0%
TCAAAATGTGCAGTGATTAT+GGG + chr4.2:91900708-91900727 None:intergenic 30.0%
TGGAAAGTTCTCTTATTGTT+TGG - chr4.2:91900326-91900345 MS.gene053153:CDS 30.0%
TTCAAAATGTGCAGTGATTA+TGG + chr4.2:91900709-91900728 None:intergenic 30.0%
TTTCTTATGACCTATTCAGT+CGG - chr4.2:91899877-91899896 MS.gene053153:CDS 30.0%
! ATAATCACTGCACATTTTGA+AGG - chr4.2:91900708-91900727 MS.gene053153:CDS 30.0%
! TTTAGCTCAACAGAATTTGT+TGG - chr4.2:91900836-91900855 MS.gene053153:CDS 30.0%
!! ACATTGAAAGCATTTAGTGT+TGG - chr4.2:91900468-91900487 MS.gene053153:CDS 30.0%
!! TACTCAATAAAGGCATCTTT+GGG - chr4.2:91900811-91900830 MS.gene053153:CDS 30.0%
!!! TTTGGGTCTTTTTTCACTAA+AGG + chr4.2:91900432-91900451 None:intergenic 30.0%
AGATTAAGAAAACCACCAGT+TGG + chr4.2:91900651-91900670 None:intergenic 35.0%
ATAAGAAACTCACCTTTAGC+GGG + chr4.2:91899865-91899884 None:intergenic 35.0%
ATCACAAGGAGATCTTAGTT+TGG - chr4.2:91900137-91900156 MS.gene053153:CDS 35.0%
CATAAGAAACTCACCTTTAG+CGG + chr4.2:91899866-91899885 None:intergenic 35.0%
GTTATGTCCAAAACCTATCA+CGG + chr4.2:91900214-91900233 None:intergenic 35.0%
TCATAATTTGGTATCAGCTG+TGG - chr4.2:91900593-91900612 MS.gene053153:CDS 35.0%
TCATTTCCTAAAACCGTGAT+AGG - chr4.2:91900198-91900217 MS.gene053153:CDS 35.0%
TGCCTTTATTGAGTAGAGAT+CGG + chr4.2:91900806-91900825 None:intergenic 35.0%
! ACCCCACCATTTAAGTTATA+TGG - chr4.2:91899901-91899920 MS.gene053153:CDS 35.0%
! CAATCAAATGGTAGTGTTGA+TGG - chr4.2:91900516-91900535 MS.gene053153:CDS 35.0%
! CTTTCTAATGCTCTAAGCAA+TGG - chr4.2:91899676-91899695 MS.gene053153:CDS 35.0%
! GGTAACATCATAGTTGATTC+AGG - chr4.2:91900537-91900556 MS.gene053153:CDS 35.0%
! TCAACGAGCAAACTCAATTT+TGG - chr4.2:91900366-91900385 MS.gene053153:CDS 35.0%
!! CTACTCAATAAAGGCATCTT+TGG - chr4.2:91900810-91900829 MS.gene053153:CDS 35.0%
!! TATGGTTTGGTACAATCAGT+AGG + chr4.2:91900900-91900919 None:intergenic 35.0%
!!! CGTTGGTATTTGTTTTGTGT+AGG + chr4.2:91899754-91899773 None:intergenic 35.0%
!!! GGTATTTGTTTTGTGTAGGT+TGG + chr4.2:91899750-91899769 None:intergenic 35.0%
AGTTAAACTGAAGCGTGCAA+AGG - chr4.2:91900623-91900642 MS.gene053153:CDS 40.0%
ATACAGGTAGTGACATGGTT+TGG - chr4.2:91899932-91899951 MS.gene053153:CDS 40.0%
CAATTGGGTTCATCAATTGG+TGG - chr4.2:91900306-91900325 MS.gene053153:CDS 40.0%
CTTACATTAGATTCCACCTC+TGG - chr4.2:91900165-91900184 MS.gene053153:CDS 40.0%
GGATTTGGGTGTGTTAGTAA+GGG + chr4.2:91899833-91899852 None:intergenic 40.0%
GTAATGATCGACATGATCGA+TGG + chr4.2:91899800-91899819 None:intergenic 40.0%
GTCCGATCTCTACTCAATAA+AGG - chr4.2:91900801-91900820 MS.gene053153:CDS 40.0%
TGAAATAGAAGAACCAGAGG+TGG + chr4.2:91900181-91900200 None:intergenic 40.0%
TGGATTTGGGTGTGTTAGTA+AGG + chr4.2:91899834-91899853 None:intergenic 40.0%
TGGTTGTAACAGTGTTCACA+AGG + chr4.2:91899970-91899989 None:intergenic 40.0%
TGTTGATACAGGTAGTGACA+TGG - chr4.2:91899927-91899946 MS.gene053153:CDS 40.0%
! CCAAAACCTATCACGGTTTT+AGG + chr4.2:91900207-91900226 None:intergenic 40.0%
! CCTAAAACCGTGATAGGTTT+TGG - chr4.2:91900204-91900223 MS.gene053153:CDS 40.0%
!! GCTCAACAGAATTTGTTGGT+TGG - chr4.2:91900840-91900859 MS.gene053153:CDS 40.0%
!! GGTGATGGATCAATATCACA+AGG - chr4.2:91900123-91900142 MS.gene053153:CDS 40.0%
!!! TTTTGTGTAGGTTGGTAGAG+TGG + chr4.2:91899742-91899761 None:intergenic 40.0%
ACCTTTGCTCGAGTTTCTGA+TGG - chr4.2:91900759-91900778 MS.gene053153:CDS 45.0%
CCTTTGCTCGAGTTTCTGAT+GGG - chr4.2:91900760-91900779 MS.gene053153:CDS 45.0%
CTTTGCTCGAGTTTCTGATG+GGG - chr4.2:91900761-91900780 MS.gene053153:CDS 45.0%
GGAGAAGCTGCTATAGTTTC+TGG - chr4.2:91900387-91900406 MS.gene053153:CDS 45.0%
TCATTTGAAGGCAAGAGCTC+TGG - chr4.2:91900243-91900262 MS.gene053153:CDS 45.0%
! TCTCTTATTGTTTGGGGCCA+TGG - chr4.2:91900334-91900353 MS.gene053153:CDS 45.0%
!! GCATAGCATTGACAACACGT+TGG + chr4.2:91899771-91899790 None:intergenic 45.0%
!! GCTGCTATAGTTTCTGGTCA+TGG - chr4.2:91900393-91900412 MS.gene053153:CDS 45.0%
!! GTGGTGATTTTGAAGAGTCG+CGG + chr4.2:91899723-91899742 None:intergenic 45.0%
AAGCGTGCAAAGGATCCAAC+TGG - chr4.2:91900633-91900652 MS.gene053153:CDS 50.0%
CCCATCAGAAACTCGAGCAA+AGG + chr4.2:91900763-91900782 None:intergenic 50.0%
CTCGTTGAGTTAGACGACCA+TGG + chr4.2:91900354-91900373 None:intergenic 50.0%
GCAGTGACAGTTGTACCATC+TGG - chr4.2:91900564-91900583 MS.gene053153:CDS 50.0%
!! GTGATTTTGAAGAGTCGCGG+TGG + chr4.2:91899720-91899739 None:intergenic 50.0%
CGTGCAAAGGATCCAACTGG+TGG - chr4.2:91900636-91900655 MS.gene053153:CDS 55.0%
GAGACACGGGTCCATTTCCA+AGG + chr4.2:91900278-91900297 None:intergenic 55.0%
TCCACTGTGATGCCCGCTAA+AGG - chr4.2:91899850-91899869 MS.gene053153:CDS 55.0%
! ACCTTTAGCGGGCATCACAG+TGG + chr4.2:91899854-91899873 None:intergenic 55.0%
! AGCGGGCATCACAGTGGATT+TGG + chr4.2:91899848-91899867 None:intergenic 55.0%
! GCGGGCATCACAGTGGATTT+GGG + chr4.2:91899847-91899866 None:intergenic 55.0%
! TGAAGGCAAGAGCTCTGGTG+TGG - chr4.2:91900248-91900267 MS.gene053153:CDS 55.0%
!! GGTGTGGTTGGCCTTGGAAA+TGG - chr4.2:91900264-91900283 MS.gene053153:CDS 55.0%
!! TGGTGGGGTACCGACTGAAT+AGG + chr4.2:91899890-91899909 None:intergenic 55.0%
! GGCAAGAGCTCTGGTGTGGT+TGG - chr4.2:91900252-91900271 MS.gene053153:CDS 60.0%
!! AGCTCTGGTGTGGTTGGCCT+TGG - chr4.2:91900258-91900277 MS.gene053153:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 91899616 91900923 91899616 ID=MS.gene053153
chr4.2 mRNA 91899616 91900923 91899616 ID=MS.gene053153.t1;Parent=MS.gene053153
chr4.2 exon 91899616 91900923 91899616 ID=MS.gene053153.t1.exon1;Parent=MS.gene053153.t1
chr4.2 CDS 91899616 91900923 91899616 ID=cds.MS.gene053153.t1;Parent=MS.gene053153.t1
Gene Sequence

>MS.gene053153

ATGAGTATGTGTTATTTTCTTGCTCTTCAGCTTTTTTCTTTTTCTTGTTTTATGATTTCTCTTTCTAATGCTCTAAGCAATGGCTTTAGTGTTGAACTCATCCACCGCGACTCTTCAAAATCACCACTCTACCAACCTACACAAAACAAATACCAACGTGTTGTCAATGCTATGCGTCGTTCCATCGATCATGTCGATCATTACTACAAACATTCCCTTACTAACACACCCAAATCCACTGTGATGCCCGCTAAAGGTGAGTTTCTTATGACCTATTCAGTCGGTACCCCACCATTTAAGTTATATGGTATTGTTGATACAGGTAGTGACATGGTTTGGCTTCAATCTAAGCCTTGTGAACACTGTTACAACCAAACGACTCCTATTTTTAATCCTTTGAATTCATCAAGTTACAAAAACAATATTCCTTGTTTGTCTAAAAAATGTAAGTCTTTTGATTCTACCTCTTGTAATAAAAACAAAACTTGCGAATATAGTATTAAGTATGGTGATGGATCAATATCACAAGGAGATCTTAGTTTGGAAACTCTTACATTAGATTCCACCTCTGGTTCTTCTATTTCATTTCCTAAAACCGTGATAGGTTTTGGACATAACAATACATTGTCATTTGAAGGCAAGAGCTCTGGTGTGGTTGGCCTTGGAAATGGACCCGTGTCTCTTATAAATCAATTGGGTTCATCAATTGGTGGAAAGTTCTCTTATTGTTTGGGGCCATGGTCGTCTAACTCAACGAGCAAACTCAATTTTGGAGAAGCTGCTATAGTTTCTGGTCATGGAGTTGTGTCAACTCCTTTAGTGAAAAAAGACCCAAAAATTGCTTACTTAATAACATTGAAAGCATTTAGTGTTGGAAACAAAAGAGTAGAATTTTCAAAGCAATCAAATGGTAGTGTTGATGGTAACATCATAGTTGATTCAGGTACAGCAGTGACAGTTGTACCATCTGGTATTTATCATAATTTGGTATCAGCTGTGGTAAAATCAGTTAAACTGAAGCGTGCAAAGGATCCAACTGGTGGTTTTCTTAATCTTTGTTATTCCGTTGCATCAAATAAATATGATTTTCCCATAATCACTGCACATTTTGAAGGTGCAGATGTTAAGTTGCATTCTATGAGCACCTTTGCTCGAGTTTCTGATGGGGTTTTTTGTTTGACTCTTGTCCGATCTCTACTCAATAAAGGCATCTTTGGGAATTTAGCTCAACAGAATTTGTTGGTTGGTTATGATCTTCAACAAAAAATTGTGTCTTTTAAGCCTACTGATTGTACCAAACCATATTGA
Protein sequence

>MS.gene053153.t1

MSMCYFLALQLFSFSCFMISLSNALSNGFSVELIHRDSSKSPLYQPTQNKYQRVVNAMRRSIDHVDHYYKHSLTNTPKSTVMPAKGEFLMTYSVGTPPFKLYGIVDTGSDMVWLQSKPCEHCYNQTTPIFNPLNSSSYKNNIPCLSKKCKSFDSTSCNKNKTCEYSIKYGDGSISQGDLSLETLTLDSTSGSSISFPKTVIGFGHNNTLSFEGKSSGVVGLGNGPVSLINQLGSSIGGKFSYCLGPWSSNSTSKLNFGEAAIVSGHGVVSTPLVKKDPKIAYLITLKAFSVGNKRVEFSKQSNGSVDGNIIVDSGTAVTVVPSGIYHNLVSAVVKSVKLKRAKDPTGGFLNLCYSVASNKYDFPIITAHFEGADVKLHSMSTFARVSDGVFCLTLVRSLLNKGIFGNLAQQNLLVGYDLQQKIVSFKPTDCTKPY