Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053434.t1 | AES67764.1 | 88.5 | 52 | 6 | 0 | 1 | 52 | 153 | 204 | 3.80E-16 | 93.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053434.t1 | A0A072VMX4 | 88.5 | 52 | 6 | 0 | 1 | 52 | 153 | 204 | 2.7e-16 | 93.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053434 | MS.gene061615 | 0.830705 | 2.53E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053434.t1 | MTR_2g098850 | 88.462 | 52 | 6 | 0 | 1 | 52 | 153 | 204 | 2.98e-25 | 95.9 |
| MS.gene053434.t1 | MTR_2g098850 | 88.462 | 52 | 6 | 0 | 1 | 52 | 153 | 204 | 3.25e-25 | 95.9 |
| MS.gene053434.t1 | MTR_4g067110 | 79.592 | 49 | 10 | 0 | 1 | 49 | 153 | 201 | 4.75e-20 | 81.3 |
| MS.gene053434.t1 | MTR_7g091780 | 67.308 | 52 | 17 | 0 | 1 | 52 | 157 | 208 | 4.42e-19 | 78.6 |
| MS.gene053434.t1 | MTR_4g039320 | 59.615 | 52 | 21 | 0 | 1 | 52 | 153 | 204 | 4.42e-14 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053434.t1 | AT1G05960 | 71.429 | 49 | 14 | 0 | 1 | 49 | 174 | 222 | 1.58e-17 | 73.9 |
| MS.gene053434.t1 | AT1G05960 | 71.429 | 49 | 14 | 0 | 1 | 49 | 153 | 201 | 1.60e-17 | 73.9 |
| MS.gene053434.t1 | AT1G05960 | 71.429 | 49 | 14 | 0 | 1 | 49 | 153 | 201 | 1.60e-17 | 73.9 |
| MS.gene053434.t1 | AT1G05960 | 71.429 | 49 | 14 | 0 | 1 | 49 | 153 | 201 | 1.60e-17 | 73.9 |
| MS.gene053434.t1 | AT2G41830 | 57.692 | 52 | 22 | 0 | 1 | 52 | 157 | 208 | 9.15e-16 | 68.9 |
| MS.gene053434.t1 | AT2G41830 | 57.692 | 52 | 22 | 0 | 1 | 52 | 22 | 73 | 9.70e-16 | 68.9 |
| MS.gene053434.t1 | AT5G21080 | 57.692 | 52 | 22 | 0 | 1 | 52 | 153 | 204 | 6.34e-14 | 63.9 |
Find 13 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAGCCAGTAGACATAGTTT+TGG | 0.333286 | 1.4:-28998627 | None:intergenic |
| ACTACAAACTCTATCTTCTA+TGG | 0.349959 | 1.4:+28998695 | MS.gene053434:CDS |
| TACATACATGTTCAACTTAG+AGG | 0.356074 | 1.4:+28998596 | None:intergenic |
| TTTGGCCAGGTGAAGTTCAT+GGG | 0.467465 | 1.4:+28999053 | MS.gene053434:intron |
| TATCCCAAAACTATGTCTAC+TGG | 0.488825 | 1.4:+28998623 | MS.gene053434:CDS |
| ACATACATGTTCAACTTAGA+GGG | 0.518274 | 1.4:+28998597 | None:intergenic |
| CTATGTCTACTGGCTCAAGA+AGG | 0.545233 | 1.4:+28998633 | MS.gene053434:CDS |
| TATGTCTACTGGCTCAAGAA+GGG | 0.555788 | 1.4:+28998634 | MS.gene053434:CDS |
| GCTCTGCTTCTACATTCAGC+AGG | 0.585336 | 1.4:+28998672 | MS.gene053434:CDS |
| GAGCACTCACATCTATCCAC+AGG | 0.607742 | 1.4:+28999077 | MS.gene053434:CDS |
| CTCACTTTGTCAAAATCCTG+TGG | 0.611988 | 1.4:-28999093 | None:intergenic |
| ATGTCTACTGGCTCAAGAAG+GGG | 0.614063 | 1.4:+28998635 | MS.gene053434:CDS |
| TGTCTACTGGCTCAAGAAGG+GGG | 0.638768 | 1.4:+28998636 | MS.gene053434:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTTTTCATAAAAATAATCA+AGG | - | chr1.4:28998830-28998849 | None:intergenic | 15.0% |
| !! | TTCATAAAAATAATCAAGGT+GGG | - | chr1.4:28998826-28998845 | None:intergenic | 20.0% |
| !! | TTTCATAAAAATAATCAAGG+TGG | - | chr1.4:28998827-28998846 | None:intergenic | 20.0% |
| !!! | GATTTTCTCTTTTGTTTTTT+TGG | + | chr1.4:28999035-28999054 | MS.gene053434:intron | 20.0% |
| ! | AGAAAATCAAAAGACATCAA+TGG | - | chr1.4:28999023-28999042 | None:intergenic | 25.0% |
| ! | TTCAGTAAGAAAGAGTAAAA+AGG | - | chr1.4:28998728-28998747 | None:intergenic | 25.0% |
| AATCAAAAGACATCAATGGA+AGG | - | chr1.4:28999019-28999038 | None:intergenic | 30.0% | |
| ACTACAAACTCTATCTTCTA+TGG | + | chr1.4:28998695-28998714 | MS.gene053434:CDS | 30.0% | |
| TGTTTAATATTCTGTGCAGA+TGG | - | chr1.4:28998855-28998874 | None:intergenic | 30.0% | |
| ACGACAAAGATCATCATTAG+CGG | + | chr1.4:28998880-28998899 | MS.gene053434:intron | 35.0% | |
| TATCCCAAAACTATGTCTAC+TGG | + | chr1.4:28998623-28998642 | MS.gene053434:CDS | 35.0% | |
| ! | TCTATATCAGTTCCCTTTTG+AGG | + | chr1.4:28998984-28999003 | MS.gene053434:intron | 35.0% |
| !!! | TCTCTTTTGTTTTTTTGGCC+AGG | + | chr1.4:28999040-28999059 | MS.gene053434:intron | 35.0% |
| AAGGCTCAAGTAACCTCAAA+AGG | - | chr1.4:28999000-28999019 | None:intergenic | 40.0% | |
| AGGCTCAAGTAACCTCAAAA+GGG | - | chr1.4:28998999-28999018 | None:intergenic | 40.0% | |
| ! | GAGCCAGTAGACATAGTTTT+GGG | - | chr1.4:28998629-28998648 | None:intergenic | 40.0% |
| ! | TGAGCCAGTAGACATAGTTT+TGG | - | chr1.4:28998630-28998649 | None:intergenic | 40.0% |
| !! | CTGATATAGAGTATCGTGGT+AGG | - | chr1.4:28998974-28998993 | None:intergenic | 40.0% |
| !! | GGAACTGATATAGAGTATCG+TGG | - | chr1.4:28998978-28998997 | None:intergenic | 40.0% |
| !! | TATGTCTACTGGCTCAAGAA+GGG | + | chr1.4:28998634-28998653 | MS.gene053434:CDS | 40.0% |
| TTTGGCCAGGTGAAGTTCAT+GGG | + | chr1.4:28999053-28999072 | MS.gene053434:intron | 45.0% | |
| ! | GAGTATCGTGGTAGGAACTA+GGG | - | chr1.4:28998966-28998985 | None:intergenic | 45.0% |
| ! | TTTTGGCCAGGTGAAGTTCA+TGG | + | chr1.4:28999052-28999071 | MS.gene053434:intron | 45.0% |
| !! | AGAGTATCGTGGTAGGAACT+AGG | - | chr1.4:28998967-28998986 | None:intergenic | 45.0% |
| !! | ATGTCTACTGGCTCAAGAAG+GGG | + | chr1.4:28998635-28998654 | MS.gene053434:CDS | 45.0% |
| !! | CTATGTCTACTGGCTCAAGA+AGG | + | chr1.4:28998633-28998652 | MS.gene053434:CDS | 45.0% |
| GAGCACTCACATCTATCCAC+AGG | + | chr1.4:28999077-28999096 | MS.gene053434:CDS | 50.0% | |
| GCTCTGCTTCTACATTCAGC+AGG | + | chr1.4:28998672-28998691 | MS.gene053434:CDS | 50.0% | |
| !! | TGTCTACTGGCTCAAGAAGG+GGG | + | chr1.4:28998636-28998655 | MS.gene053434:CDS | 50.0% |
| GCTCACCCATGAACTTCACC+TGG | - | chr1.4:28999061-28999080 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 28998603 | 28999106 | 28998603 | ID=MS.gene053434 |
| chr1.4 | mRNA | 28998603 | 28999106 | 28998603 | ID=MS.gene053434.t1;Parent=MS.gene053434 |
| chr1.4 | exon | 28998603 | 28998716 | 28998603 | ID=MS.gene053434.t1.exon1;Parent=MS.gene053434.t1 |
| chr1.4 | CDS | 28998603 | 28998716 | 28998603 | ID=cds.MS.gene053434.t1;Parent=MS.gene053434.t1 |
| chr1.4 | exon | 28999062 | 28999106 | 28999062 | ID=MS.gene053434.t1.exon2;Parent=MS.gene053434.t1 |
| chr1.4 | CDS | 28999062 | 28999106 | 28999062 | ID=cds.MS.gene053434.t1;Parent=MS.gene053434.t1 |
| Gene Sequence |
| Protein sequence |
