Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061615.t1 | AES67764.1 | 88.5 | 52 | 6 | 0 | 1 | 52 | 153 | 204 | 3.80E-16 | 93.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061615.t1 | A0A072VMX4 | 88.5 | 52 | 6 | 0 | 1 | 52 | 153 | 204 | 2.7e-16 | 93.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051210 | MS.gene061615 | 0.802013 | 6.86E-49 | -1.69E-46 |
| MS.gene051800 | MS.gene061615 | 0.812389 | 4.35E-51 | -1.69E-46 |
| MS.gene052896 | MS.gene061615 | 0.800246 | 1.58E-48 | -1.69E-46 |
| MS.gene053434 | MS.gene061615 | 0.830705 | 2.53E-55 | -1.69E-46 |
| MS.gene055926 | MS.gene061615 | 0.817284 | 3.57E-52 | -1.69E-46 |
| MS.gene05604 | MS.gene061615 | 0.811518 | 6.73E-51 | -1.69E-46 |
| MS.gene057915 | MS.gene061615 | 0.821501 | 3.91E-53 | -1.69E-46 |
| MS.gene06052 | MS.gene061615 | 0.803691 | 3.09E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061615.t1 | MTR_2g098850 | 88.462 | 52 | 6 | 0 | 1 | 52 | 153 | 204 | 2.98e-25 | 95.9 |
| MS.gene061615.t1 | MTR_2g098850 | 88.462 | 52 | 6 | 0 | 1 | 52 | 153 | 204 | 3.25e-25 | 95.9 |
| MS.gene061615.t1 | MTR_4g067110 | 79.592 | 49 | 10 | 0 | 1 | 49 | 153 | 201 | 4.75e-20 | 81.3 |
| MS.gene061615.t1 | MTR_7g091780 | 67.308 | 52 | 17 | 0 | 1 | 52 | 157 | 208 | 4.42e-19 | 78.6 |
| MS.gene061615.t1 | MTR_4g039320 | 59.615 | 52 | 21 | 0 | 1 | 52 | 153 | 204 | 4.42e-14 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061615.t1 | AT1G05960 | 71.429 | 49 | 14 | 0 | 1 | 49 | 174 | 222 | 1.58e-17 | 73.9 |
| MS.gene061615.t1 | AT1G05960 | 71.429 | 49 | 14 | 0 | 1 | 49 | 153 | 201 | 1.60e-17 | 73.9 |
| MS.gene061615.t1 | AT1G05960 | 71.429 | 49 | 14 | 0 | 1 | 49 | 153 | 201 | 1.60e-17 | 73.9 |
| MS.gene061615.t1 | AT1G05960 | 71.429 | 49 | 14 | 0 | 1 | 49 | 153 | 201 | 1.60e-17 | 73.9 |
| MS.gene061615.t1 | AT2G41830 | 57.692 | 52 | 22 | 0 | 1 | 52 | 157 | 208 | 9.15e-16 | 68.9 |
| MS.gene061615.t1 | AT2G41830 | 57.692 | 52 | 22 | 0 | 1 | 52 | 22 | 73 | 9.70e-16 | 68.9 |
| MS.gene061615.t1 | AT5G21080 | 57.692 | 52 | 22 | 0 | 1 | 52 | 153 | 204 | 6.34e-14 | 63.9 |
Find 13 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAGCCAGTAGACATAGTTT+TGG | 0.333286 | 1.2:-27558732 | None:intergenic |
| ACTACAAACTCTATCTTCTA+TGG | 0.349959 | 1.2:+27558800 | MS.gene061615:CDS |
| TACATACATGTTCAACTTAG+AGG | 0.356074 | 1.2:+27558701 | None:intergenic |
| TTTGGCCAGGTGAAGTTCAT+GGG | 0.467465 | 1.2:+27559159 | MS.gene061615:intron |
| TATCCCAAAACTATGTCTAC+TGG | 0.488825 | 1.2:+27558728 | MS.gene061615:CDS |
| ACATACATGTTCAACTTAGA+GGG | 0.518274 | 1.2:+27558702 | None:intergenic |
| CTATGTCTACTGGCTCAAGA+AGG | 0.545233 | 1.2:+27558738 | MS.gene061615:CDS |
| TATGTCTACTGGCTCAAGAA+GGG | 0.555788 | 1.2:+27558739 | MS.gene061615:CDS |
| GCTCTGCTTCTACATTCAGC+AGG | 0.585336 | 1.2:+27558777 | MS.gene061615:CDS |
| GAGCACTCACATCTATCCAC+AGG | 0.607742 | 1.2:+27559183 | MS.gene061615:CDS |
| CTCACTTTGTCAAAATCCTG+TGG | 0.611988 | 1.2:-27559199 | None:intergenic |
| ATGTCTACTGGCTCAAGAAG+GGG | 0.614063 | 1.2:+27558740 | MS.gene061615:CDS |
| TGTCTACTGGCTCAAGAAGG+GGG | 0.638768 | 1.2:+27558741 | MS.gene061615:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTTTTCATAAAAATAATCA+AGG | - | chr1.2:27558935-27558954 | None:intergenic | 15.0% |
| !! | TTCATAAAAATAATCAAGGT+GGG | - | chr1.2:27558931-27558950 | None:intergenic | 20.0% |
| !! | TTTCATAAAAATAATCAAGG+TGG | - | chr1.2:27558932-27558951 | None:intergenic | 20.0% |
| ! | AGAAAATCAAAAGACATCAA+TGG | - | chr1.2:27559129-27559148 | None:intergenic | 25.0% |
| ! | TTCAGTAAGAAAGAGTAAAA+AGG | - | chr1.2:27558833-27558852 | None:intergenic | 25.0% |
| !!! | GATTTTCTCCTTTGTTTTTT+TGG | + | chr1.2:27559141-27559160 | MS.gene061615:intron | 25.0% |
| ACTACAAACTCTATCTTCTA+TGG | + | chr1.2:27558800-27558819 | MS.gene061615:CDS | 30.0% | |
| ATCAAAAGACATCAATGGAA+AGG | - | chr1.2:27559124-27559143 | None:intergenic | 30.0% | |
| TGTTTAATATTCTGTGCAGA+TGG | - | chr1.2:27558960-27558979 | None:intergenic | 30.0% | |
| ACGACAAAGATCATCATTAG+CGG | + | chr1.2:27558985-27559004 | MS.gene061615:intron | 35.0% | |
| TATCCCAAAACTATGTCTAC+TGG | + | chr1.2:27558728-27558747 | MS.gene061615:CDS | 35.0% | |
| ! | TCTATATCAGTTCCCTTTTG+AGG | + | chr1.2:27559089-27559108 | MS.gene061615:intron | 35.0% |
| AAGGCTCAAGTAACCTCAAA+AGG | - | chr1.2:27559105-27559124 | None:intergenic | 40.0% | |
| AGGCTCAAGTAACCTCAAAA+GGG | - | chr1.2:27559104-27559123 | None:intergenic | 40.0% | |
| TCACCTGGCCAAAAAAACAA+AGG | - | chr1.2:27559152-27559171 | None:intergenic | 40.0% | |
| ! | GAGCCAGTAGACATAGTTTT+GGG | - | chr1.2:27558734-27558753 | None:intergenic | 40.0% |
| ! | TGAGCCAGTAGACATAGTTT+TGG | - | chr1.2:27558735-27558754 | None:intergenic | 40.0% |
| !! | CTGATATAGAGTATCGTGGT+AGG | - | chr1.2:27559079-27559098 | None:intergenic | 40.0% |
| !! | GGAACTGATATAGAGTATCG+TGG | - | chr1.2:27559083-27559102 | None:intergenic | 40.0% |
| !! | TATGTCTACTGGCTCAAGAA+GGG | + | chr1.2:27558739-27558758 | MS.gene061615:CDS | 40.0% |
| !!! | TCTCCTTTGTTTTTTTGGCC+AGG | + | chr1.2:27559146-27559165 | MS.gene061615:intron | 40.0% |
| TTTGGCCAGGTGAAGTTCAT+GGG | + | chr1.2:27559159-27559178 | MS.gene061615:intron | 45.0% | |
| ! | GAGTATCGTGGTAGGAACTA+GGG | - | chr1.2:27559071-27559090 | None:intergenic | 45.0% |
| ! | TTTTGGCCAGGTGAAGTTCA+TGG | + | chr1.2:27559158-27559177 | MS.gene061615:intron | 45.0% |
| !! | AGAGTATCGTGGTAGGAACT+AGG | - | chr1.2:27559072-27559091 | None:intergenic | 45.0% |
| !! | ATGTCTACTGGCTCAAGAAG+GGG | + | chr1.2:27558740-27558759 | MS.gene061615:CDS | 45.0% |
| !! | CTATGTCTACTGGCTCAAGA+AGG | + | chr1.2:27558738-27558757 | MS.gene061615:CDS | 45.0% |
| GAGCACTCACATCTATCCAC+AGG | + | chr1.2:27559183-27559202 | MS.gene061615:CDS | 50.0% | |
| GCTCTGCTTCTACATTCAGC+AGG | + | chr1.2:27558777-27558796 | MS.gene061615:CDS | 50.0% | |
| !! | TGTCTACTGGCTCAAGAAGG+GGG | + | chr1.2:27558741-27558760 | MS.gene061615:CDS | 50.0% |
| GCTCACCCATGAACTTCACC+TGG | - | chr1.2:27559167-27559186 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 27558708 | 27559212 | 27558708 | ID=MS.gene061615 |
| chr1.2 | mRNA | 27558708 | 27559212 | 27558708 | ID=MS.gene061615.t1;Parent=MS.gene061615 |
| chr1.2 | exon | 27558708 | 27558821 | 27558708 | ID=MS.gene061615.t1.exon1;Parent=MS.gene061615.t1 |
| chr1.2 | CDS | 27558708 | 27558821 | 27558708 | ID=cds.MS.gene061615.t1;Parent=MS.gene061615.t1 |
| chr1.2 | exon | 27559168 | 27559212 | 27559168 | ID=MS.gene061615.t1.exon2;Parent=MS.gene061615.t1 |
| chr1.2 | CDS | 27559168 | 27559212 | 27559168 | ID=cds.MS.gene061615.t1;Parent=MS.gene061615.t1 |
| Gene Sequence |
| Protein sequence |
