AlfalfaGEDB | MS.gene053679

M. sativa cultivar XinJiangDaYe / MS.gene053679

KEGG: K08790
GO: GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005524 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564
PFAM: PF00069.28

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053679.t1	AES60251.1	74.1	108	20	1	2	109	241	340	2.30E-34	154.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053679.t1	Q2LZZ7	41.7	108	51	1	2	109	218	313	1.0e-14	80.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053679.t1	G7I4M5	74.1	108	20	1	2	109	241	340	1.7e-34	154.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049975	MS.gene053679	0.896982	2.19E-76	-1.69E-46
MS.gene053677	MS.gene053679	0.831378	1.73E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053679.t1	MTR_1g042700	84.259	108	9	1	2	109	241	340	8.67e-57	183
MS.gene053679.t1	MTR_1g442790	76.852	108	17	1	2	109	236	335	7.00e-51	167
MS.gene053679.t1	MTR_1g442790	76.852	108	17	1	2	109	236	335	7.69e-51	168
MS.gene053679.t1	MTR_1g075880	67.593	108	26	2	2	109	243	341	8.37e-45	152
MS.gene053679.t1	MTR_1g069735	60.185	108	33	3	2	109	246	343	1.39e-37	132
MS.gene053679.t1	MTR_1g069735	60.185	108	33	3	2	109	246	343	2.09e-37	131
MS.gene053679.t1	MTR_1g069735	60.185	108	33	3	2	109	246	343	4.01e-37	132
MS.gene053679.t1	MTR_7g097150	61.111	108	32	3	2	109	246	343	6.97e-37	130
MS.gene053679.t1	MTR_2g040150	53.043	115	39	4	2	109	239	345	4.07e-29	109
MS.gene053679.t1	MTR_3g104700	50.459	109	51	3	2	109	220	326	2.23e-28	107
MS.gene053679.t1	MTR_3g104700	50.459	109	51	3	2	109	220	326	2.59e-28	107
MS.gene053679.t1	MTR_1g019340	51.852	108	44	3	2	109	234	333	1.03e-27	105
MS.gene053679.t1	MTR_1g019340	51.852	108	44	3	2	109	234	333	1.63e-27	105
MS.gene053679.t1	MTR_2g040180	75.862	29	7	0	80	108	1	29	6.01e-12	58.5
MS.gene053679.t1	MTR_6g470940	34.545	110	49	4	2	109	1003	1091	7.29e-12	60.8
MS.gene053679.t1	MTR_4g035835	36.607	112	43	5	2	109	885	972	1.49e-11	60.1
MS.gene053679.t1	MTR_5g069000	36.607	112	43	5	2	109	889	976	1.50e-11	60.1
MS.gene053679.t1	MTR_2g040265	73.333	30	8	0	80	109	1	30	1.62e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053679.t1	AT4G14350	62.037	108	32	2	2	109	245	343	2.54e-39	137
MS.gene053679.t1	AT4G14350	62.037	108	32	2	2	109	245	343	2.93e-39	137
MS.gene053679.t1	AT4G14350	62.037	108	32	2	2	109	245	343	2.93e-39	137
MS.gene053679.t1	AT4G14350	62.037	108	32	2	2	109	245	343	2.93e-39	137
MS.gene053679.t1	AT3G23310	61.818	110	31	3	2	109	246	346	7.27e-38	134
MS.gene053679.t1	AT2G20470	57.407	108	35	2	2	109	250	346	3.30e-36	129
MS.gene053679.t1	AT2G20470	57.407	108	35	2	2	109	250	346	5.19e-36	129
MS.gene053679.t1	AT2G20470	57.407	108	35	2	2	109	250	346	5.19e-36	129
MS.gene053679.t1	AT1G03920	58.333	108	35	3	2	109	150	247	1.07e-33	121
MS.gene053679.t1	AT1G03920	58.333	108	35	3	2	109	263	360	2.42e-33	121
MS.gene053679.t1	AT1G03920	58.333	108	35	3	2	109	263	360	2.42e-33	121
MS.gene053679.t1	AT4G33080	51.327	113	44	3	2	109	220	326	7.25e-30	111
MS.gene053679.t1	AT4G33080	51.327	113	44	3	2	109	220	326	7.93e-30	111
MS.gene053679.t1	AT2G19400	52.212	113	43	3	2	109	231	337	2.08e-28	107
MS.gene053679.t1	AT5G09890	54.955	111	38	5	2	109	228	329	1.76e-27	105
MS.gene053679.t1	AT5G09890	54.955	111	38	5	2	109	228	329	1.90e-27	105
MS.gene053679.t1	AT1G30640	46.847	111	48	3	2	109	153	255	1.15e-25	99.8
MS.gene053679.t1	AT1G30640	46.847	111	48	3	2	109	246	348	3.69e-25	98.6
MS.gene053679.t1	AT1G30640	46.847	111	48	3	2	109	246	348	3.69e-25	98.6
MS.gene053679.t1	AT1G48490	34.259	108	46	2	2	109	955	1037	6.39e-12	61.2
MS.gene053679.t1	AT1G48490	34.259	108	46	2	2	109	954	1036	6.78e-12	61.2
MS.gene053679.t1	AT1G48490	34.259	108	46	2	2	109	954	1036	6.78e-12	61.2
MS.gene053679.t1	AT1G48490	34.259	108	46	2	2	109	954	1036	6.78e-12	61.2
MS.gene053679.t1	AT5G62310	37.963	108	48	6	2	109	880	968	9.97e-12	60.5
MS.gene053679.t1	AT3G17850	34.259	108	52	2	2	109	1008	1096	1.05e-11	60.5
MS.gene053679.t1	AT1G45160	33.028	109	50	3	2	110	796	881	4.38e-11	58.5
MS.gene053679.t1	AT1G45160	33.028	109	50	3	2	110	796	881	4.79e-11	58.5

Find 24 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACGTGAAATTATCAGATTT+TGG	0.253070	5.1:-47630676	MS.gene053679:CDS
CCAGTTGCTGTTGTTGTGAT+TGG	0.300638	5.1:+47630524	None:intergenic
CATGCCCGACAACTTGCTTC+TGG	0.340707	5.1:-47630710	MS.gene053679:CDS
GTGATTGGTATAATAGTATA+TGG	0.352774	5.1:-47630002	MS.gene053679:CDS
AAAAGACTGCAGTAACCTTC+AGG	0.370471	5.1:-47630617	MS.gene053679:CDS
GCTAGACTGCAGTAACCTTC+AGG	0.390289	5.1:-47630641	MS.gene053679:CDS
CCTCTCTTCAGAATAACTTC+TGG	0.412241	5.1:+47630039	None:intergenic
AAGCAAGTTCATCACTTTGA+AGG	0.415965	5.1:+47630557	None:intergenic
CCAATCACAACAACAGCAAC+TGG	0.416983	5.1:-47630524	MS.gene053679:CDS
GAGTGTTCCAACCGTAGAAT+AGG	0.473047	5.1:+47630074	None:intergenic
TGCTGTTGTTGTGATTGGTT+AGG	0.484482	5.1:+47630529	None:intergenic
TCTAATGGAGTTAACATAAG+TGG	0.486303	5.1:-47630583	MS.gene053679:CDS
AACAGCAACTGGAGCATTCG+CGG	0.503673	5.1:-47630513	MS.gene053679:CDS
CTATCCAGAAGCAAGTTGTC+GGG	0.515263	5.1:+47630706	None:intergenic
GATATTGCAGACCTATTCTA+CGG	0.522525	5.1:-47630085	MS.gene053679:intron
TCTATCCAGAAGCAAGTTGT+CGG	0.524432	5.1:+47630705	None:intergenic
TTGCAGACCTATTCTACGGT+TGG	0.534089	5.1:-47630081	MS.gene053679:intron
GTTATTCTGAAGAGAGGGTA+TGG	0.540943	5.1:-47630033	MS.gene053679:CDS
AACTTGCTTCTGGATAGAAA+TGG	0.545939	5.1:-47630700	MS.gene053679:CDS
GGTATGGCGTGGAATGTGAT+TGG	0.559029	5.1:-47630017	MS.gene053679:CDS
CCAGAAGTTATTCTGAAGAG+AGG	0.595321	5.1:-47630039	MS.gene053679:CDS
CAGAAGTTATTCTGAAGAGA+GGG	0.602815	5.1:-47630038	MS.gene053679:CDS
TGAAGGTTACTGCAGTCTAG+CGG	0.630065	5.1:+47630643	None:intergenic
TCTGAAGAGAGGGTATGGCG+TGG	0.686438	5.1:-47630028	MS.gene053679:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTATTATAACTGAAAAA+AGG	+	chr5.1:47630392-47630411	None:intergenic	10.0%
!!	ATTTACATCATATATCAATT+TGG	-	chr5.1:47630491-47630510	MS.gene053679:intron	15.0%
!!	TAGCAAACTTTATTATAAAA+CGG	-	chr5.1:47630290-47630309	MS.gene053679:intron	15.0%
!!	TTTACATCATATATCAATTT+GGG	-	chr5.1:47630492-47630511	MS.gene053679:CDS	15.0%
!!	TATGATTACTTTATTCAAAG+CGG	-	chr5.1:47630360-47630379	MS.gene053679:intron	20.0%
!	AAAGAGAGAATAGTCTTTAA+AGG	+	chr5.1:47630537-47630556	None:intergenic	25.0%
!	CATTATTCTTTCGTTATGTT+TGG	-	chr5.1:47630418-47630437	MS.gene053679:intron	25.0%
!	GTGATTGGTATAATAGTATA+TGG	-	chr5.1:47630703-47630722	MS.gene053679:CDS	25.0%
!!	TTATAACTGAAAAAAGGTGT+AGG	+	chr5.1:47630386-47630405	None:intergenic	25.0%
!!!	TATTCTCTCTTTTGATTGTA+TGG	-	chr5.1:47630545-47630564	MS.gene053679:CDS	25.0%
!!!	TCTTTCGTGATATTTTTGTT+TGG	-	chr5.1:47630449-47630468	MS.gene053679:intron	25.0%
	CAGGAAAAAGACTTATCTAA+TGG	-	chr5.1:47630107-47630126	MS.gene053679:intron	30.0%
	TCTAATGGAGTTAACATAAG+TGG	-	chr5.1:47630122-47630141	MS.gene053679:intron	30.0%
!	CACGTGAAATTATCAGATTT+TGG	-	chr5.1:47630029-47630048	MS.gene053679:CDS	30.0%
!	TAGATAAGTCTTTTTCCTGA+AGG	+	chr5.1:47630106-47630125	None:intergenic	30.0%
	AAGCAAGTTCATCACTTTGA+AGG	+	chr5.1:47630151-47630170	None:intergenic	35.0%
	CTTTCGTTATGTTTGGACTT+CGG	-	chr5.1:47630425-47630444	MS.gene053679:intron	35.0%
	GATATTGCAGACCTATTCTA+CGG	-	chr5.1:47630620-47630639	MS.gene053679:CDS	35.0%
!	AACTTGCTTCTGGATAGAAA+TGG	-	chr5.1:47630005-47630024	MS.gene053679:CDS	35.0%
!	CAGAAGTTATTCTGAAGAGA+GGG	-	chr5.1:47630667-47630686	MS.gene053679:CDS	35.0%
	AAAAGACTGCAGTAACCTTC+AGG	-	chr5.1:47630088-47630107	MS.gene053679:intron	40.0%
	CCTCTCTTCAGAATAACTTC+TGG	+	chr5.1:47630669-47630688	None:intergenic	40.0%
	TCTATCCAGAAGCAAGTTGT+CGG	+	chr5.1:47630003-47630022	None:intergenic	40.0%
!	CCAGAAGTTATTCTGAAGAG+AGG	-	chr5.1:47630666-47630685	MS.gene053679:CDS	40.0%
!	GTTATTCTGAAGAGAGGGTA+TGG	-	chr5.1:47630672-47630691	MS.gene053679:CDS	40.0%
!	TACTGCAGTCTTTTTCCTGA+AGG	+	chr5.1:47630082-47630101	None:intergenic	40.0%
!	TGCTGTTGTTGTGATTGGTT+AGG	+	chr5.1:47630179-47630198	None:intergenic	40.0%
	CCAATCACAACAACAGCAAC+TGG	-	chr5.1:47630181-47630200	MS.gene053679:intron	45.0%
	CTATCCAGAAGCAAGTTGTC+GGG	+	chr5.1:47630002-47630021	None:intergenic	45.0%
	GAAAAATCGCAGCAGAATGC+TGG	-	chr5.1:47630214-47630233	MS.gene053679:intron	45.0%
	GAGTGTTCCAACCGTAGAAT+AGG	+	chr5.1:47630634-47630653	None:intergenic	45.0%
	TGAAGGTTACTGCAGTCTAG+CGG	+	chr5.1:47630065-47630084	None:intergenic	45.0%
	TTGCAGACCTATTCTACGGT+TGG	-	chr5.1:47630624-47630643	MS.gene053679:CDS	45.0%
!	CCAGTTGCTGTTGTTGTGAT+TGG	+	chr5.1:47630184-47630203	None:intergenic	45.0%
	GCTAGACTGCAGTAACCTTC+AGG	-	chr5.1:47630064-47630083	MS.gene053679:CDS	50.0%
	GGTATGGCGTGGAATGTGAT+TGG	-	chr5.1:47630688-47630707	MS.gene053679:CDS	50.0%
!	AACAGCAACTGGAGCATTCG+CGG	-	chr5.1:47630192-47630211	MS.gene053679:intron	50.0%
!	TCTGAAGAGAGGGTATGGCG+TGG	-	chr5.1:47630677-47630696	MS.gene053679:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	47629996	47630731	47629996	ID=MS.gene053679
chr5.1	mRNA	47629996	47630731	47629996	ID=MS.gene053679.t1;Parent=MS.gene053679
chr5.1	exon	47630492	47630731	47630492	ID=MS.gene053679.t1.exon1;Parent=MS.gene053679.t1
chr5.1	CDS	47630492	47630731	47630492	ID=cds.MS.gene053679.t1;Parent=MS.gene053679.t1
chr5.1	exon	47629996	47630097	47629996	ID=MS.gene053679.t1.exon2;Parent=MS.gene053679.t1
chr5.1	CDS	47629996	47630097	47629996	ID=cds.MS.gene053679.t1;Parent=MS.gene053679.t1

Gene Sequence

>MS.gene053679

ATGCCCGACAACTTGCTTCTGGATAGAAATGGTCACGTGAAATTATCAGATTTTGGATTATGTAAGCCGCTAGACTGCAGTAACCTTCAGGAAAAAGACTGCAGTAACCTTCAGGAAAAAGACTTATCTAATGGAGTTAACATAAGTGGAGTCCTTCAAAGTGATGAACTTGCTTTATCTCCTAACCAATCACAACAACAGCAACTGGAGCATTCGCGGAAAAATCGCAGCAGAATGCTGGTAAGTCTCTCAAATAAACACATTCATAAATAAGCATGATTTTTTTTTTTAACATAGCAAACTTTATTATAAAACGGCGAGACTCACAACACATGAGTACTCACAAAACGTATTCATAAATAAGTATGATTACTTTATTCAAAGCGGCCTACACCTTTTTTCAGTTATAATAAAAATTCAGTCATTATTCTTTCGTTATGTTTGGACTTCGGTTCTTTCGTGATATTTTTGTTTGGTTAGTACGAGTCTAGCATCATTTACATCATATATCAATTTGGGTTATTGCTTTATATTTTATCCTTTAAAGACTATTCTCTCTTTTGATTGTATGGCTGCTCTACTTAGATTCATCATTAATGACATTTTAAGCTTTTCTATCTTTGTGATATTGCAGACCTATTCTACGGTTGGAACACTCGATTATATTGCTCCAGAAGTTATTCTGAAGAGAGGGTATGGCGTGGAATGTGATTGGTATAATAGTATATGGCGTTGA

Protein sequence

>MS.gene053679.t1

MPDNLLLDRNGHVKLSDFGLCKPLDCSNLQEKDCSNLQEKDLSNGVNISGVLQSDELALSPNQSQQQQLEHSRKNRSRMLTYSTVGTLDYIAPEVILKRGYGVECDWYNSIWR