Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054182.t1 | XP_013452188.1 | 80.2 | 116 | 16 | 1 | 1 | 109 | 22 | 137 | 9.70E-46 | 192.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054182.t1 | P62929 | 39.7 | 116 | 55 | 7 | 2 | 109 | 22 | 130 | 1.3e-11 | 70.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054182.t1 | A0A072U9W9 | 80.2 | 116 | 16 | 1 | 1 | 109 | 22 | 137 | 7.0e-46 | 192.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054182 | MS.gene059401 | 0.833034 | 6.72E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054182.t1 | MTR_6g047880 | 80.172 | 116 | 16 | 1 | 1 | 109 | 22 | 137 | 4.37e-62 | 186 |
| MS.gene054182.t1 | MTR_6g047900 | 78.761 | 113 | 20 | 3 | 1 | 109 | 22 | 134 | 5.15e-57 | 172 |
| MS.gene054182.t1 | MTR_7g029540 | 62.245 | 98 | 32 | 2 | 1 | 93 | 24 | 121 | 1.75e-36 | 120 |
| MS.gene054182.t1 | MTR_3g463570 | 50.459 | 109 | 47 | 3 | 1 | 103 | 23 | 130 | 1.15e-23 | 88.6 |
| MS.gene054182.t1 | MTR_3g463570 | 50.459 | 109 | 47 | 3 | 1 | 103 | 43 | 150 | 2.38e-23 | 88.2 |
| MS.gene054182.t1 | MTR_3g067270 | 44.545 | 110 | 55 | 2 | 1 | 105 | 22 | 130 | 2.86e-22 | 84.7 |
| MS.gene054182.t1 | MTR_3g467750 | 44.545 | 110 | 55 | 2 | 1 | 105 | 22 | 130 | 2.86e-22 | 84.7 |
| MS.gene054182.t1 | MTR_7g056817 | 43.478 | 115 | 54 | 4 | 1 | 105 | 22 | 135 | 5.92e-22 | 84.3 |
| MS.gene054182.t1 | MTR_3g067280 | 42.727 | 110 | 57 | 2 | 1 | 105 | 22 | 130 | 9.10e-21 | 80.9 |
| MS.gene054182.t1 | MTR_3g467760 | 47.500 | 80 | 39 | 1 | 29 | 105 | 27 | 106 | 7.12e-20 | 78.2 |
| MS.gene054182.t1 | MTR_7g056803 | 45.614 | 114 | 52 | 5 | 1 | 105 | 22 | 134 | 3.19e-19 | 77.0 |
| MS.gene054182.t1 | MTR_6g036620 | 40.000 | 110 | 58 | 2 | 2 | 105 | 23 | 130 | 4.77e-19 | 76.6 |
| MS.gene054182.t1 | MTR_3g067550 | 41.379 | 116 | 56 | 3 | 1 | 105 | 1 | 115 | 9.09e-19 | 75.5 |
| MS.gene054182.t1 | MTR_3g067555 | 41.379 | 116 | 56 | 3 | 1 | 105 | 1 | 115 | 9.09e-19 | 75.5 |
| MS.gene054182.t1 | MTR_7g044980 | 44.898 | 98 | 44 | 4 | 1 | 92 | 21 | 114 | 1.01e-18 | 76.3 |
| MS.gene054182.t1 | MTR_3g067540 | 41.379 | 116 | 56 | 3 | 1 | 105 | 22 | 136 | 1.05e-18 | 75.9 |
| MS.gene054182.t1 | MTR_3g067555 | 41.379 | 116 | 56 | 3 | 1 | 105 | 22 | 136 | 1.05e-18 | 75.9 |
| MS.gene054182.t1 | MTR_3g067555 | 41.379 | 116 | 56 | 3 | 1 | 105 | 9 | 123 | 1.22e-18 | 75.5 |
| MS.gene054182.t1 | MTR_3g067535 | 42.982 | 114 | 53 | 4 | 1 | 105 | 22 | 132 | 2.23e-18 | 75.1 |
| MS.gene054182.t1 | MTR_6g017150 | 48.718 | 78 | 37 | 2 | 29 | 105 | 58 | 133 | 7.12e-18 | 73.6 |
| MS.gene054182.t1 | MTR_3g067500 | 38.739 | 111 | 61 | 3 | 1 | 105 | 1 | 110 | 8.81e-18 | 72.8 |
| MS.gene054182.t1 | MTR_3g067437 | 39.815 | 108 | 59 | 2 | 1 | 102 | 22 | 129 | 2.10e-17 | 72.4 |
| MS.gene054182.t1 | MTR_8g022400 | 40.741 | 81 | 47 | 1 | 23 | 102 | 50 | 130 | 8.86e-17 | 71.2 |
| MS.gene054182.t1 | MTR_3g436100 | 40.000 | 95 | 44 | 3 | 28 | 109 | 59 | 153 | 1.57e-16 | 70.5 |
| MS.gene054182.t1 | MTR_3g067580 | 39.286 | 112 | 60 | 4 | 1 | 105 | 30 | 140 | 6.76e-16 | 68.9 |
| MS.gene054182.t1 | MTR_8g022420 | 41.975 | 81 | 46 | 1 | 23 | 102 | 49 | 129 | 7.35e-16 | 68.6 |
| MS.gene054182.t1 | MTR_3g067570 | 44.211 | 95 | 42 | 5 | 1 | 88 | 1 | 91 | 1.10e-15 | 67.0 |
| MS.gene054182.t1 | MTR_8g022430 | 40.659 | 91 | 51 | 3 | 19 | 108 | 48 | 136 | 1.19e-15 | 68.2 |
| MS.gene054182.t1 | MTR_6g017170 | 39.806 | 103 | 53 | 4 | 1 | 95 | 22 | 123 | 3.97e-15 | 66.2 |
| MS.gene054182.t1 | MTR_1g019650 | 43.299 | 97 | 47 | 3 | 1 | 96 | 22 | 111 | 4.15e-15 | 65.9 |
| MS.gene054182.t1 | MTR_3g067430 | 37.391 | 115 | 62 | 3 | 1 | 105 | 22 | 136 | 1.12e-14 | 65.5 |
| MS.gene054182.t1 | MTR_7g044920 | 42.029 | 69 | 40 | 0 | 38 | 106 | 67 | 135 | 5.58e-14 | 63.9 |
| MS.gene054182.t1 | MTR_3g067510 | 38.938 | 113 | 53 | 5 | 1 | 105 | 27 | 131 | 6.01e-14 | 63.5 |
| MS.gene054182.t1 | MTR_3g067510 | 38.938 | 113 | 53 | 5 | 1 | 105 | 22 | 126 | 6.27e-14 | 63.5 |
| MS.gene054182.t1 | MTR_4g026590 | 35.897 | 117 | 63 | 2 | 1 | 105 | 23 | 139 | 9.50e-14 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACAAACCATAATCGACATTA+GGG | + | 43358:37071-37090 | None:intergenic | 30.0% | |
| ACAAGATCTTTATATGATGC+AGG | + | 43358:36958-36977 | None:intergenic | 30.0% | |
| ! | CAAAAGTTAGTATTTTTCCC+AGG | + | 43358:36988-37007 | None:intergenic | 30.0% |
| AAAGATCTTGTGAAGATACC+TGG | - | 43358:36967-36986 | MS.gene054182:CDS | 35.0% | |
| AAGATCTTGTGAAGATACCT+GGG | - | 43358:36968-36987 | MS.gene054182:CDS | 35.0% | |
| AGAAGCAAATCGTTGTTATC+TGG | - | 43358:36867-36886 | MS.gene054182:CDS | 35.0% | |
| CACAAACCATAATCGACATT+AGG | + | 43358:37072-37091 | None:intergenic | 35.0% | |
| GATCTTTATATGATGCAGGT+TGG | + | 43358:36954-36973 | None:intergenic | 35.0% | |
| ! | TAACTTTTGTCAGTCTCATG+TGG | - | 43358:36999-37018 | MS.gene054182:CDS | 35.0% |
| !!! | TTTTGGGACTGTAGATTTAG+AGG | + | 43358:37136-37155 | None:intergenic | 35.0% |
| ATCTTCTGCCTCAGATAAAG+AGG | + | 43358:37100-37119 | None:intergenic | 40.0% | |
| CATGTGGAATGCAAAGAGAA+GGG | - | 43358:37015-37034 | MS.gene054182:CDS | 40.0% | |
| TCATGTGGAATGCAAAGAGA+AGG | - | 43358:37014-37033 | MS.gene054182:CDS | 40.0% | |
| ! | AGCATGGTCAAGAAGTCTTT+TGG | + | 43358:37153-37172 | None:intergenic | 40.0% |
| ! | GCATGGTCAAGAAGTCTTTT+GGG | + | 43358:37152-37171 | None:intergenic | 40.0% |
| AATGCAAAGAGAAGGGACGT+GGG | - | 43358:37022-37041 | MS.gene054182:CDS | 45.0% | |
| CCATAATCGACATTAGGGCT+AGG | + | 43358:37066-37085 | None:intergenic | 45.0% | |
| GGCGTAGGTTGTGTTTGTTT+CGG | - | 43358:36919-36938 | MS.gene054182:CDS | 45.0% | |
| GTGTGTTGCCTCTTTATCTG+AGG | - | 43358:37089-37108 | MS.gene054182:CDS | 45.0% | |
| ! | CCTAGCCCTAATGTCGATTA+TGG | - | 43358:37063-37082 | MS.gene054182:CDS | 45.0% |
| GAATGCAAAGAGAAGGGACG+TGG | - | 43358:37021-37040 | MS.gene054182:CDS | 50.0% | |
| TCAGACACATCCCGAACATG+CGG | - | 43358:36898-36917 | MS.gene054182:CDS | 50.0% | |
| ACAACCTACGCCGCATGTTC+GGG | + | 43358:36911-36930 | None:intergenic | 55.0% | |
| CACAACCTACGCCGCATGTT+CGG | + | 43358:36912-36931 | None:intergenic | 55.0% | |
| ACATCCCGAACATGCGGCGT+AGG | - | 43358:36904-36923 | MS.gene054182:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 43358 | gene | 36856 | 37185 | 36856 | ID=MS.gene054182 |
| 43358 | mRNA | 36856 | 37185 | 36856 | ID=MS.gene054182.t1;Parent=MS.gene054182 |
| 43358 | exon | 36856 | 37185 | 36856 | ID=MS.gene054182.t1.exon1;Parent=MS.gene054182.t1 |
| 43358 | CDS | 36856 | 37185 | 36856 | ID=cds.MS.gene054182.t1;Parent=MS.gene054182.t1 |
| Gene Sequence |
| Protein sequence |
