Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059401.t1 | XP_013452188.1 | 81 | 116 | 15 | 1 | 1 | 109 | 22 | 137 | 3.30E-46 | 194.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059401.t1 | P62929 | 39.7 | 116 | 55 | 7 | 2 | 109 | 22 | 130 | 1.3e-11 | 70.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059401.t1 | A0A072U9W9 | 81.0 | 116 | 15 | 1 | 1 | 109 | 22 | 137 | 2.4e-46 | 194.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054181 | MS.gene059401 | 0.833165 | 6.24E-56 | -1.69E-46 |
| MS.gene054182 | MS.gene059401 | 0.833034 | 6.72E-56 | -1.69E-46 |
| MS.gene059401 | MS.gene059402 | 0.801252 | 9.83E-49 | -1.69E-46 |
| MS.gene059401 | MS.gene26578 | 0.80083 | 1.20E-48 | -1.69E-46 |
| MS.gene059401 | MS.gene30725 | 0.814 | 1.93E-51 | -1.69E-46 |
| MS.gene059401 | MS.gene89774 | 0.80321 | 3.89E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059401.t1 | MTR_6g047880 | 81.034 | 116 | 15 | 1 | 1 | 109 | 22 | 137 | 7.64e-63 | 187 |
| MS.gene059401.t1 | MTR_6g047900 | 77.876 | 113 | 21 | 3 | 1 | 109 | 22 | 134 | 2.64e-56 | 171 |
| MS.gene059401.t1 | MTR_7g029540 | 62.245 | 98 | 32 | 2 | 1 | 93 | 24 | 121 | 1.62e-36 | 120 |
| MS.gene059401.t1 | MTR_3g463570 | 50.459 | 109 | 47 | 3 | 1 | 103 | 23 | 130 | 6.61e-24 | 89.0 |
| MS.gene059401.t1 | MTR_3g463570 | 50.459 | 109 | 47 | 3 | 1 | 103 | 43 | 150 | 1.58e-23 | 88.6 |
| MS.gene059401.t1 | MTR_3g067270 | 43.636 | 110 | 56 | 2 | 1 | 105 | 22 | 130 | 1.01e-21 | 83.6 |
| MS.gene059401.t1 | MTR_3g467750 | 43.636 | 110 | 56 | 2 | 1 | 105 | 22 | 130 | 1.01e-21 | 83.6 |
| MS.gene059401.t1 | MTR_7g056817 | 42.609 | 115 | 55 | 4 | 1 | 105 | 22 | 135 | 2.67e-21 | 82.4 |
| MS.gene059401.t1 | MTR_3g067280 | 41.818 | 110 | 58 | 2 | 1 | 105 | 22 | 130 | 1.27e-20 | 80.9 |
| MS.gene059401.t1 | MTR_3g467760 | 47.500 | 80 | 39 | 1 | 29 | 105 | 27 | 106 | 6.60e-20 | 78.2 |
| MS.gene059401.t1 | MTR_6g036620 | 40.000 | 110 | 58 | 2 | 2 | 105 | 23 | 130 | 2.63e-19 | 77.4 |
| MS.gene059401.t1 | MTR_3g067550 | 41.379 | 116 | 56 | 3 | 1 | 105 | 1 | 115 | 7.89e-19 | 75.5 |
| MS.gene059401.t1 | MTR_3g067555 | 41.379 | 116 | 56 | 3 | 1 | 105 | 1 | 115 | 7.89e-19 | 75.5 |
| MS.gene059401.t1 | MTR_7g044980 | 44.898 | 98 | 44 | 4 | 1 | 92 | 21 | 114 | 8.31e-19 | 76.3 |
| MS.gene059401.t1 | MTR_3g067540 | 41.379 | 116 | 56 | 3 | 1 | 105 | 22 | 136 | 9.06e-19 | 75.9 |
| MS.gene059401.t1 | MTR_3g067555 | 41.379 | 116 | 56 | 3 | 1 | 105 | 22 | 136 | 9.06e-19 | 75.9 |
| MS.gene059401.t1 | MTR_3g067555 | 41.379 | 116 | 56 | 3 | 1 | 105 | 9 | 123 | 9.64e-19 | 75.5 |
| MS.gene059401.t1 | MTR_7g056803 | 43.860 | 114 | 54 | 4 | 1 | 105 | 22 | 134 | 1.22e-18 | 75.5 |
| MS.gene059401.t1 | MTR_3g067500 | 39.640 | 111 | 60 | 3 | 1 | 105 | 1 | 110 | 1.77e-18 | 74.7 |
| MS.gene059401.t1 | MTR_3g067535 | 42.982 | 114 | 53 | 4 | 1 | 105 | 22 | 132 | 3.25e-18 | 74.7 |
| MS.gene059401.t1 | MTR_6g017150 | 48.718 | 78 | 37 | 2 | 29 | 105 | 58 | 133 | 6.75e-18 | 73.9 |
| MS.gene059401.t1 | MTR_3g067437 | 39.815 | 108 | 59 | 2 | 1 | 102 | 22 | 129 | 1.06e-17 | 73.2 |
| MS.gene059401.t1 | MTR_8g022400 | 40.000 | 90 | 52 | 2 | 14 | 102 | 42 | 130 | 7.70e-17 | 71.2 |
| MS.gene059401.t1 | MTR_3g436100 | 40.000 | 95 | 44 | 3 | 28 | 109 | 59 | 153 | 1.77e-16 | 70.5 |
| MS.gene059401.t1 | MTR_3g067570 | 44.211 | 95 | 42 | 5 | 1 | 88 | 1 | 91 | 6.76e-16 | 67.4 |
| MS.gene059401.t1 | MTR_8g022420 | 44.000 | 75 | 41 | 1 | 29 | 102 | 55 | 129 | 7.35e-16 | 68.6 |
| MS.gene059401.t1 | MTR_3g067580 | 39.286 | 112 | 60 | 4 | 1 | 105 | 30 | 140 | 8.29e-16 | 68.6 |
| MS.gene059401.t1 | MTR_8g022430 | 41.975 | 81 | 45 | 2 | 29 | 108 | 57 | 136 | 1.26e-15 | 68.2 |
| MS.gene059401.t1 | MTR_6g017170 | 39.806 | 103 | 53 | 4 | 1 | 95 | 22 | 123 | 3.24e-15 | 66.6 |
| MS.gene059401.t1 | MTR_1g019650 | 43.299 | 97 | 47 | 3 | 1 | 96 | 22 | 111 | 5.56e-15 | 65.5 |
| MS.gene059401.t1 | MTR_3g067430 | 37.391 | 115 | 62 | 3 | 1 | 105 | 22 | 136 | 1.06e-14 | 65.5 |
| MS.gene059401.t1 | MTR_3g067510 | 38.938 | 113 | 53 | 5 | 1 | 105 | 27 | 131 | 5.12e-14 | 63.5 |
| MS.gene059401.t1 | MTR_3g067510 | 38.938 | 113 | 53 | 5 | 1 | 105 | 22 | 126 | 5.57e-14 | 63.5 |
| MS.gene059401.t1 | MTR_7g044920 | 42.029 | 69 | 40 | 0 | 38 | 106 | 67 | 135 | 5.95e-14 | 63.9 |
| MS.gene059401.t1 | MTR_4g026590 | 35.897 | 117 | 63 | 2 | 1 | 105 | 23 | 139 | 7.67e-14 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTAGTCCTAATGTTGATTA+TGG | 0.176691 | 6.3:-42591052 | MS.gene059401:CDS |
| AGCATGGTCAAGAAGTCTTT+TGG | 0.276295 | 6.3:+42590965 | None:intergenic |
| CACAACCTACACCGCATGTT+TGG | 0.290947 | 6.3:+42591206 | None:intergenic |
| AGAAGCAAACCGTTGTTATC+TGG | 0.377036 | 6.3:-42591248 | MS.gene059401:CDS |
| ACAACCTACACCGCATGTTT+GGG | 0.389533 | 6.3:+42591207 | None:intergenic |
| ACAAGATCTTTATATGATGC+AGG | 0.450360 | 6.3:+42591160 | None:intergenic |
| CACAAACCATAATCAACATT+AGG | 0.450638 | 6.3:+42591046 | None:intergenic |
| AAAGATCTTGTGAAGATACC+TGG | 0.482787 | 6.3:-42591148 | MS.gene059401:CDS |
| TTTGCTTCTACTTTCTTCAT+CGG | 0.487039 | 6.3:+42591262 | None:intergenic |
| ATCTTCTGCCTCAGATAAAG+AGG | 0.487203 | 6.3:+42591018 | None:intergenic |
| GGTGTAGGTTGTGTTTGTTT+CGG | 0.512311 | 6.3:-42591196 | MS.gene059401:CDS |
| GATCTTTATATGATGCAGGT+TGG | 0.554280 | 6.3:+42591164 | None:intergenic |
| GAATGCAAAGAGAAGGGACG+TGG | 0.558645 | 6.3:-42591094 | MS.gene059401:CDS |
| TCATGTGGAATGCAAAGAGA+AGG | 0.568615 | 6.3:-42591101 | MS.gene059401:CDS |
| GTGTGTTGCCTCTTTATCTG+AGG | 0.572934 | 6.3:-42591026 | MS.gene059401:CDS |
| ACATCCCAAACATGCGGTGT+AGG | 0.582167 | 6.3:-42591211 | MS.gene059401:CDS |
| CCATAATCAACATTAGGACT+AGG | 0.583229 | 6.3:+42591052 | None:intergenic |
| AAGATCTTGTGAAGATACCT+GGG | 0.596931 | 6.3:-42591147 | MS.gene059401:CDS |
| ATATCAAGCTATCTCAAGCA+TGG | 0.614801 | 6.3:+42590949 | None:intergenic |
| CATGTGGAATGCAAAGAGAA+GGG | 0.628774 | 6.3:-42591100 | MS.gene059401:CDS |
| AATGCAAAGAGAAGGGACGT+GGG | 0.703464 | 6.3:-42591093 | MS.gene059401:CDS |
| TCAGAGACATCCCAAACATG+CGG | 0.712944 | 6.3:-42591217 | MS.gene059401:CDS |
| AACAAACAGCCAGATAACAA+CGG | 0.721899 | 6.3:+42591239 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACAAGATCTTTATATGATGC+AGG | + | chr6.3:42591054-42591073 | None:intergenic | 30.0% | |
| CACAAACCATAATCAACATT+AGG | + | chr6.3:42591168-42591187 | None:intergenic | 30.0% | |
| ! | CAAAAGTTAGTATTTTTCCC+AGG | + | chr6.3:42591084-42591103 | None:intergenic | 30.0% |
| AAAGATCTTGTGAAGATACC+TGG | - | chr6.3:42591063-42591082 | MS.gene059401:CDS | 35.0% | |
| AACAAACAGCCAGATAACAA+CGG | + | chr6.3:42590975-42590994 | None:intergenic | 35.0% | |
| AAGATCTTGTGAAGATACCT+GGG | - | chr6.3:42591064-42591083 | MS.gene059401:CDS | 35.0% | |
| CCATAATCAACATTAGGACT+AGG | + | chr6.3:42591162-42591181 | None:intergenic | 35.0% | |
| GATCTTTATATGATGCAGGT+TGG | + | chr6.3:42591050-42591069 | None:intergenic | 35.0% | |
| ! | CCTAGTCCTAATGTTGATTA+TGG | - | chr6.3:42591159-42591178 | MS.gene059401:CDS | 35.0% |
| ! | TAACTTTTGTCAGTCTCATG+TGG | - | chr6.3:42591095-42591114 | MS.gene059401:CDS | 35.0% |
| !!! | TTTTGGGACTGTAGATTTAG+AGG | + | chr6.3:42591232-42591251 | None:intergenic | 35.0% |
| AGAAGCAAACCGTTGTTATC+TGG | - | chr6.3:42590963-42590982 | MS.gene059401:CDS | 40.0% | |
| ATCTTCTGCCTCAGATAAAG+AGG | + | chr6.3:42591196-42591215 | None:intergenic | 40.0% | |
| CATGTGGAATGCAAAGAGAA+GGG | - | chr6.3:42591111-42591130 | MS.gene059401:CDS | 40.0% | |
| GGTGTAGGTTGTGTTTGTTT+CGG | - | chr6.3:42591015-42591034 | MS.gene059401:CDS | 40.0% | |
| TCATGTGGAATGCAAAGAGA+AGG | - | chr6.3:42591110-42591129 | MS.gene059401:CDS | 40.0% | |
| ! | AGCATGGTCAAGAAGTCTTT+TGG | + | chr6.3:42591249-42591268 | None:intergenic | 40.0% |
| ! | GCATGGTCAAGAAGTCTTTT+GGG | + | chr6.3:42591248-42591267 | None:intergenic | 40.0% |
| AATGCAAAGAGAAGGGACGT+GGG | - | chr6.3:42591118-42591137 | MS.gene059401:CDS | 45.0% | |
| ACAACCTACACCGCATGTTT+GGG | + | chr6.3:42591007-42591026 | None:intergenic | 45.0% | |
| GTGTGTTGCCTCTTTATCTG+AGG | - | chr6.3:42591185-42591204 | MS.gene059401:CDS | 45.0% | |
| TCAGAGACATCCCAAACATG+CGG | - | chr6.3:42590994-42591013 | MS.gene059401:CDS | 45.0% | |
| ACATCCCAAACATGCGGTGT+AGG | - | chr6.3:42591000-42591019 | MS.gene059401:CDS | 50.0% | |
| CACAACCTACACCGCATGTT+TGG | + | chr6.3:42591008-42591027 | None:intergenic | 50.0% | |
| GAATGCAAAGAGAAGGGACG+TGG | - | chr6.3:42591117-42591136 | MS.gene059401:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 42590952 | 42591281 | 42590952 | ID=MS.gene059401 |
| chr6.3 | mRNA | 42590952 | 42591281 | 42590952 | ID=MS.gene059401.t1;Parent=MS.gene059401 |
| chr6.3 | exon | 42590952 | 42591281 | 42590952 | ID=MS.gene059401.t1.exon1;Parent=MS.gene059401.t1 |
| chr6.3 | CDS | 42590952 | 42591281 | 42590952 | ID=cds.MS.gene059401.t1;Parent=MS.gene059401.t1 |
| Gene Sequence |
| Protein sequence |
