AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054563

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054563.t1 MTR_8g096990 69.415 376 111 3 2 375 58 431 0.0 521
MS.gene054563.t1 MTR_5g080960 68.519 378 113 4 2 375 58 433 0.0 511
MS.gene054563.t1 MTR_5g080890 67.989 378 115 4 2 375 58 433 3.91e-179 504
MS.gene054563.t1 MTR_5g044770 68.351 376 114 4 2 375 58 430 1.41e-178 503
MS.gene054563.t1 MTR_8g038280 66.053 380 121 3 2 375 58 435 2.39e-177 500
MS.gene054563.t1 MTR_8g038250 66.230 382 97 5 2 375 58 415 5.69e-173 488
MS.gene054563.t1 MTR_8g038310 58.355 377 138 3 1 377 58 415 7.12e-158 450
MS.gene054563.t1 MTR_6g090290 60.733 382 141 4 1 375 57 436 2.05e-150 432
MS.gene054563.t1 MTR_4g109760 61.538 377 116 4 2 375 48 398 2.83e-147 422
MS.gene054563.t1 MTR_3g028120 54.047 383 164 7 3 375 58 438 1.35e-136 397
MS.gene054563.t1 MTR_2g067830 54.427 384 163 9 1 375 26 406 3.57e-127 372
MS.gene054563.t1 MTR_0024s0040 50.773 388 173 9 1 375 28 410 1.44e-118 350
MS.gene054563.t1 MTR_5g038360 49.741 386 179 10 1 375 55 436 8.07e-118 349
MS.gene054563.t1 MTR_6g088760 53.429 350 149 8 3 341 59 405 3.87e-115 345
MS.gene054563.t1 MTR_2g105300 50.656 381 154 10 1 372 26 381 6.87e-113 334
MS.gene054563.t1 MTR_5g044570 44.819 386 172 11 1 375 56 411 2.61e-99 301
MS.gene054563.t1 MTR_5g008070 42.971 377 203 8 6 374 53 425 1.42e-92 284
MS.gene054563.t1 MTR_2g059490 45.480 354 182 9 30 374 58 409 2.33e-91 281
MS.gene054563.t1 MTR_2g059460 42.632 380 198 12 7 375 36 406 9.59e-90 276
MS.gene054563.t1 MTR_0036s0070 40.106 379 209 9 3 375 60 426 1.78e-89 276
MS.gene054563.t1 MTR_0036s0050 40.885 384 208 9 3 375 60 435 5.92e-89 275
MS.gene054563.t1 MTR_0036s0110 40.437 366 199 8 21 375 85 442 7.23e-86 267
MS.gene054563.t1 MTR_5g008150 42.297 357 190 10 29 375 62 412 1.95e-83 260
MS.gene054563.t1 MTR_5g008110 41.457 357 192 10 29 375 66 415 1.27e-82 258
MS.gene054563.t1 MTR_1g060750 36.340 377 208 9 5 375 26 376 7.16e-70 224
MS.gene054563.t1 MTR_3g097150 36.975 357 208 9 28 375 100 448 1.60e-66 218
MS.gene054563.t1 MTR_4g045857 44.534 247 129 6 15 254 43 288 4.15e-58 191
MS.gene054563.t1 MTR_5g008090 37.667 300 172 9 86 375 20 314 1.59e-54 182
MS.gene054563.t1 MTR_1g039270 32.768 354 220 10 28 373 126 469 4.32e-52 180
MS.gene054563.t1 MTR_6g088765 42.259 239 106 5 105 342 3 210 4.44e-49 165
MS.gene054563.t1 MTR_1g115210 33.428 353 218 10 28 373 144 486 4.59e-49 172
MS.gene054563.t1 MTR_4g008110 73.684 114 9 1 103 216 45 137 5.83e-46 154
MS.gene054563.t1 MTR_7g117430 32.865 356 219 12 28 373 139 484 7.55e-46 164
MS.gene054563.t1 MTR_3g046300 32.812 384 236 11 12 375 79 460 1.75e-42 154
MS.gene054563.t1 MTR_1g111350 30.595 353 224 10 28 373 129 467 5.63e-42 153
MS.gene054563.t1 MTR_6g085010 31.322 348 223 9 30 373 156 491 7.78e-40 147
MS.gene054563.t1 MTR_3g053710 31.967 366 224 13 23 373 111 466 3.45e-37 140
MS.gene054563.t1 MTR_5g091130 30.208 384 245 11 12 374 173 554 1.19e-36 140
MS.gene054563.t1 MTR_2g028190 32.249 369 224 14 21 373 93 451 2.31e-36 137
MS.gene054563.t1 MTR_4g007740 30.259 347 208 12 47 374 147 478 3.45e-36 137
MS.gene054563.t1 MTR_8g103880 28.926 363 230 14 10 360 96 442 5.95e-32 125
MS.gene054563.t1 MTR_2g055840 26.525 377 244 12 22 373 5 373 4.10e-28 113
MS.gene054563.t1 MTR_3g068000 28.424 387 220 16 28 373 71 441 4.35e-28 115
MS.gene054563.t1 MTR_3g053980 29.268 369 238 14 17 373 128 485 1.37e-25 108
MS.gene054563.t1 MTR_2g070430 29.155 367 217 13 37 370 94 450 4.87e-25 106
MS.gene054563.t1 MTR_7g105850 29.323 399 210 19 29 375 89 467 6.96e-24 102
MS.gene054563.t1 MTR_7g080230 26.440 382 238 17 23 374 63 431 2.51e-22 98.6
MS.gene054563.t1 MTR_4g023650 26.396 394 236 17 16 375 48 421 6.19e-22 97.1
MS.gene054563.t1 MTR_3g065160 24.619 394 242 15 18 375 54 428 6.35e-22 97.1
MS.gene054563.t1 MTR_5g016260 25.137 366 238 14 28 375 86 433 4.55e-21 95.1
MS.gene054563.t1 MTR_5g016260 25.668 374 230 16 28 377 86 435 7.03e-21 94.7
MS.gene054563.t1 MTR_4g095270 25.405 370 232 15 28 375 78 425 4.84e-20 92.0
MS.gene054563.t1 MTR_8g094590 25.729 377 235 18 23 373 67 424 5.09e-20 91.3
MS.gene054563.t1 MTR_4g095270 25.405 370 232 15 28 375 78 425 5.50e-20 92.0
MS.gene054563.t1 MTR_1g029810 25.849 383 232 18 23 375 51 411 5.55e-20 91.3
MS.gene054563.t1 MTR_0945s0010 37.903 124 74 3 253 374 1 123 6.50e-20 86.3
MS.gene054563.t1 MTR_1g029810 25.849 383 232 18 23 375 51 411 7.12e-20 90.9
MS.gene054563.t1 MTR_7g062360 26.893 383 229 16 15 360 44 412 8.19e-20 90.9
MS.gene054563.t1 MTR_3g014290 29.118 340 214 14 31 360 81 403 4.69e-19 88.6
MS.gene054563.t1 MTR_1g037630 29.118 340 214 14 31 360 93 415 6.99e-19 88.2
MS.gene054563.t1 MTR_7g021510 24.403 377 241 17 29 374 71 434 9.08e-19 87.8
MS.gene054563.t1 MTR_4g023630 25.628 398 234 19 16 375 48 421 2.09e-18 86.7
MS.gene054563.t1 MTR_4g056420 27.968 379 223 19 29 374 76 437 4.50e-18 85.9
MS.gene054563.t1 MTR_3g096930 24.147 381 239 16 23 375 78 436 5.41e-18 85.5
MS.gene054563.t1 MTR_2g084550 28.382 377 225 18 29 374 76 438 6.10e-18 85.5
MS.gene054563.t1 MTR_1g078240 25.310 403 257 18 5 374 39 430 1.90e-17 84.0
MS.gene054563.t1 MTR_1g078240 26.173 405 252 19 5 374 39 431 3.63e-17 83.2
MS.gene054563.t1 MTR_4g100990 26.214 412 223 18 30 374 74 471 8.14e-17 82.0
MS.gene054563.t1 MTR_8g094590 26.331 338 208 15 23 338 67 385 8.47e-17 81.6
MS.gene054563.t1 MTR_5g077850 22.775 382 234 14 31 375 82 439 1.34e-16 81.3
MS.gene054563.t1 MTR_8g094590 28.295 258 158 10 23 272 67 305 1.69e-16 79.7
MS.gene054563.t1 MTR_8g039540 29.326 341 213 15 31 360 97 420 3.92e-16 79.7
MS.gene054563.t1 MTR_1g078240 26.409 337 211 15 5 315 39 364 1.19e-15 78.2
MS.gene054563.t1 MTR_5g061690 27.066 351 203 20 37 360 108 432 1.94e-15 78.2
MS.gene054563.t1 MTR_5g012490 25.183 409 230 19 30 374 74 470 2.13e-15 77.8
MS.gene054563.t1 MTR_3g087780 26.757 370 213 19 37 373 106 450 2.21e-15 77.8
MS.gene054563.t1 MTR_7g080230 25.429 350 219 16 54 374 25 361 3.22e-15 77.0
MS.gene054563.t1 MTR_5g022880 26.519 362 224 16 36 373 118 461 1.09e-14 75.9
MS.gene054563.t1 MTR_3g100500 28.653 349 214 16 29 361 96 425 3.18e-13 70.9
MS.gene054563.t1 MTR_2g103340 71.795 39 11 0 42 80 67 105 2.01e-12 65.9
MS.gene054563.t1 MTR_3g087780 27.019 322 192 16 73 373 9 308 1.18e-11 65.9
MS.gene054563.t1 MTR_5g061690 28.169 284 161 16 92 360 38 293 1.67e-11 65.5
MS.gene054563.t1 MTR_2g037710 27.700 213 127 10 169 373 12 205 1.99e-11 63.5
MS.gene054563.t1 MTR_8g075090 25.543 368 222 19 36 375 118 461 2.74e-11 65.5
MS.gene054563.t1 MTR_3g087780 27.019 322 192 16 73 373 45 344 2.96e-11 65.1
MS.gene054563.t1 MTR_5g080330 25.698 358 198 17 47 373 2 322 6.04e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054563.t1 AT1G64830 46.214 383 195 6 1 375 51 430 2.89e-112 335
MS.gene054563.t1 AT5G33340 48.158 380 189 7 1 375 60 436 1.21e-109 328
MS.gene054563.t1 AT2G35615 43.990 391 197 10 3 374 57 444 7.21e-96 293
MS.gene054563.t1 AT1G31450 43.188 389 198 9 3 374 60 442 2.05e-88 274
MS.gene054563.t1 AT2G28010 42.582 364 183 10 19 377 53 395 3.10e-84 261
MS.gene054563.t1 AT2G28040 42.857 364 181 13 19 377 53 394 6.92e-81 253
MS.gene054563.t1 AT2G28040 43.213 361 178 13 19 374 44 382 8.84e-81 252
MS.gene054563.t1 AT2G28030 41.311 351 182 9 31 377 61 391 6.65e-78 245
MS.gene054563.t1 AT2G28220 41.690 355 179 9 31 377 67 401 5.16e-77 243
MS.gene054563.t1 AT2G28225 38.692 367 193 10 19 375 55 399 6.38e-70 225
MS.gene054563.t1 AT2G03200 37.037 378 209 12 17 375 93 460 1.59e-59 199
MS.gene054563.t1 AT1G01300 34.454 357 214 12 28 374 139 485 8.72e-49 172
MS.gene054563.t1 AT4G30030 34.890 364 206 15 25 373 72 419 6.67e-46 162
MS.gene054563.t1 AT3G18490 31.481 378 235 13 6 373 137 500 1.09e-45 164
MS.gene054563.t1 AT1G25510 31.534 352 222 9 28 373 145 483 2.05e-43 157
MS.gene054563.t1 AT3G61820 34.097 349 204 12 28 360 132 470 3.17e-42 154
MS.gene054563.t1 AT2G23945 29.700 367 224 13 31 375 96 450 9.98e-42 152
MS.gene054563.t1 AT3G20015 30.532 357 223 12 28 373 128 470 2.30e-41 151
MS.gene054563.t1 AT5G10770 33.770 382 221 16 9 374 109 474 3.53e-40 148
MS.gene054563.t1 AT5G10760 31.105 389 239 11 1 373 89 464 7.83e-39 144
MS.gene054563.t1 AT1G79720 34.066 364 203 16 31 373 135 482 2.62e-38 143
MS.gene054563.t1 AT2G42980 30.890 382 238 14 16 373 145 524 1.34e-36 139
MS.gene054563.t1 AT3G12700 31.579 361 229 12 29 374 104 461 3.65e-36 137
MS.gene054563.t1 AT4G30040 31.117 376 210 18 19 368 75 427 3.33e-35 134
MS.gene054563.t1 AT3G25700 29.365 378 232 14 28 376 81 452 5.39e-35 134
MS.gene054563.t1 AT3G59080 30.971 381 239 16 16 374 155 533 2.56e-34 133
MS.gene054563.t1 AT5G36260 28.877 374 223 18 29 374 76 434 1.97e-31 124
MS.gene054563.t1 AT2G39710 27.704 379 221 16 28 368 62 425 1.56e-26 110
MS.gene054563.t1 AT3G59080 28.457 376 219 13 16 374 155 497 2.38e-25 107
MS.gene054563.t1 AT5G43100 27.027 370 225 15 27 374 72 418 1.04e-24 105
MS.gene054563.t1 AT3G02740 27.100 369 235 16 29 374 83 440 2.06e-24 104
MS.gene054563.t1 AT5G43100 27.027 370 225 15 27 374 72 418 2.22e-24 105
MS.gene054563.t1 AT3G02740 27.424 361 228 16 37 374 16 365 3.38e-24 103
MS.gene054563.t1 AT3G54400 28.412 359 221 16 31 374 88 425 3.71e-24 103
MS.gene054563.t1 AT5G02190 26.455 378 217 17 40 373 82 442 2.03e-23 101
MS.gene054563.t1 AT1G65240 26.415 371 233 15 29 374 72 427 9.32e-23 99.8
MS.gene054563.t1 AT1G66180 23.810 378 234 14 31 375 72 428 3.80e-22 97.8
MS.gene054563.t1 AT1G09750 25.581 387 249 13 11 373 77 448 9.10e-22 96.7
MS.gene054563.t1 AT5G45120 27.027 407 222 19 31 373 83 478 5.16e-21 94.7
MS.gene054563.t1 AT5G37540 25.600 375 231 15 31 373 80 438 1.78e-20 92.8
MS.gene054563.t1 AT3G50050 24.796 367 239 12 28 375 90 438 2.18e-20 93.2
MS.gene054563.t1 AT4G12920 27.778 360 213 19 31 373 58 387 2.38e-18 86.3
MS.gene054563.t1 AT1G05840 25.668 374 238 15 29 374 78 439 2.56e-17 83.6
MS.gene054563.t1 AT5G07030 29.050 358 195 17 31 360 115 441 2.23e-16 80.9
MS.gene054563.t1 AT3G52500 27.681 401 215 20 29 374 88 468 2.67e-16 80.5
MS.gene054563.t1 AT5G24820 25.103 243 165 6 143 377 170 403 1.47e-14 75.1
MS.gene054563.t1 AT1G77480 26.209 393 219 22 23 375 60 421 1.74e-14 75.1
MS.gene054563.t1 AT1G77480 26.209 393 219 22 23 375 60 421 1.75e-14 74.7
MS.gene054563.t1 AT3G51350 26.158 367 221 18 36 373 107 452 2.39e-14 74.7
MS.gene054563.t1 AT1G08210 25.532 376 234 19 29 374 82 441 5.28e-14 73.6
MS.gene054563.t1 AT1G08210 25.532 376 234 19 29 374 116 475 6.49e-14 73.6
MS.gene054563.t1 AT1G08210 25.532 376 234 19 29 374 130 489 7.53e-14 73.2
MS.gene054563.t1 AT2G17760 25.620 363 223 17 36 373 109 449 3.65e-13 71.2
MS.gene054563.t1 AT1G08210 27.097 310 189 16 29 315 82 377 5.31e-12 67.4
MS.gene054563.t1 AT3G42550 21.429 364 215 10 31 374 78 390 6.54e-12 67.0
MS.gene054563.t1 AT5G10080 25.946 370 223 21 37 375 106 455 1.12e-11 66.6
MS.gene054563.t1 AT3G51340 24.377 361 229 17 36 373 108 447 8.12e-11 63.9

Find 81 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTGGTATTTATCATAATT+TGG 0.131312 4.1:-87500609 MS.gene054563:CDS
TGAAGGCATCTTTGGGAATT+TGG 0.131735 4.1:-87500375 MS.gene054563:CDS
GGAGAAGCTGCTGTAGTTTC+TGG 0.166013 4.1:-87500803 MS.gene054563:CDS
CGACTCAATGAAGGCATCTT+TGG 0.201942 4.1:-87500383 MS.gene054563:CDS
TTAAAATGTGCAGTGATTAT+AGG 0.219892 4.1:+87500488 None:intergenic
GGAAAGTTCTCTTATTGTTT+GGG 0.226977 4.1:-87500863 MS.gene054563:CDS
CCTAAAACTGTGATAGGTTT+TGG 0.228752 4.1:-87500986 MS.gene054563:CDS
TCGACGAGCAAACTCAATTT+TGG 0.229402 4.1:-87500824 MS.gene054563:CDS
ACCCCGCCATTTAAGTTATA+TGG 0.232605 4.1:-87501289 MS.gene054563:CDS
TGGAAAGTTCTCTTATTGTT+TGG 0.261051 4.1:-87500864 MS.gene054563:CDS
ACAATACCATATAACTTAAA+TGG 0.265952 4.1:+87501283 None:intergenic
AGTGGGGATCACATTGGATT+TGG 0.281086 4.1:+87501345 None:intergenic
TCATTTCCTAAAACTGTGAT+AGG 0.301005 4.1:-87500992 MS.gene054563:CDS
TGCTGATGGGGTTGTCTGTT+TGG 0.306514 4.1:-87500420 MS.gene054563:CDS
CGTGTCTCTTATAAATCAAT+TGG 0.312361 4.1:-87500900 MS.gene054563:CDS
GTGGGGATCACATTGGATTT+GGG 0.335142 4.1:+87501346 None:intergenic
AACAAAGTCTGCACTCAATA+TGG 0.340167 4.1:+87500278 None:intergenic
AATCAATTGGGTTCATCAAT+TGG 0.341653 4.1:-87500887 MS.gene054563:CDS
GGTAACATCATGGTTGATTC+AGG 0.351027 4.1:-87500653 MS.gene054563:CDS
CAAAGGTTTAAATAACCATT+TGG 0.354673 4.1:+87500542 None:intergenic
TGGATTTGGGTGTGTTAGTA+AGG 0.374259 4.1:+87501359 None:intergenic
GACTCAATGAAGGCATCTTT+GGG 0.379134 4.1:-87500382 MS.gene054563:CDS
CATAAGAAACTCACCGTTAG+TGG 0.387341 4.1:+87501327 None:intergenic
ATACAGGTAGCGACATAGTT+TGG 0.387408 4.1:-87501258 MS.gene054563:CDS
GGTGTGGTTGGCCTTGGAAA+TGG 0.390490 4.1:-87500926 MS.gene054563:CDS
CAATCAAATGGTAGTGTTGA+TGG 0.396941 4.1:-87500674 MS.gene054563:CDS
AGCTCTGGTGTGGTTGGCCT+TGG 0.398793 4.1:-87500932 MS.gene054563:CDS
ACCTTTGTTTCAGTTGCTGA+TGG 0.408371 4.1:-87500434 MS.gene054563:CDS
ATCACAAGGAGATCTTAGTT+TGG 0.415779 4.1:-87501053 MS.gene054563:CDS
CCTTTGTTTCAGTTGCTGAT+GGG 0.416283 4.1:-87500433 MS.gene054563:CDS
TTTGGCTCAACAAAATTTGT+TGG 0.424190 4.1:-87500357 MS.gene054563:CDS
GATTAAAGATAGGAGTCGTT+TGG 0.435507 4.1:+87501203 None:intergenic
TTATATGGTATTGTTGATAC+AGG 0.443331 4.1:-87501274 MS.gene054563:CDS
GATTTCAAAGGATTAAAGAT+AGG 0.448590 4.1:+87501193 None:intergenic
TACCATATAACTTAAATGGC+GGG 0.451714 4.1:+87501287 None:intergenic
GGAATTAGCTGTGGTAGAAT+CGG 0.458402 4.1:-87500588 MS.gene054563:CDS
GCTCAACAAAATTTGTTGGT+TGG 0.467228 4.1:-87500353 MS.gene054563:CDS
CTTACATTAGATTCCACCTC+TGG 0.468740 4.1:-87501025 MS.gene054563:CDS
TGGCGGGGTACCGACTGAAT+AGG 0.482486 4.1:+87501303 None:intergenic
GGTTAGACTAAAGCGTGTTA+AGG 0.486147 4.1:-87500567 MS.gene054563:CDS
GGCAAGAGCTCTGGTGTGGT+TGG 0.491732 4.1:-87500938 MS.gene054563:CDS
GTGTCTCTTATAAATCAATT+GGG 0.493038 4.1:-87500899 MS.gene054563:CDS
CTTTGTTTCAGTTGCTGATG+GGG 0.494820 4.1:-87500432 MS.gene054563:CDS
TCATTTGAAGGCAAGAGCTC+TGG 0.501352 4.1:-87500947 MS.gene054563:CDS
AGTCTGCACTCAATATGGTT+TGG 0.515365 4.1:+87500283 None:intergenic
TTGTAACTTGATGATTTCAA+AGG 0.516263 4.1:+87501181 None:intergenic
GTCCCATCTCGACTCAATGA+AGG 0.517181 4.1:-87500392 MS.gene054563:CDS
GAATTTGCAAAGCAATCAAA+TGG 0.522533 4.1:-87500686 MS.gene054563:CDS
ATGCCTTCATTGAGTCGAGA+TGG 0.524151 4.1:+87500389 None:intergenic
ACATTGACAACTGTACCATC+TGG 0.525694 4.1:-87500626 MS.gene054563:CDS
CCCATCAGCAACTGAAACAA+AGG 0.528788 4.1:+87500433 None:intergenic
GTAATGATCGACACGGTTGA+TGG 0.531409 4.1:+87501393 None:intergenic
GCTGCTGTAGTTTCTGGTCA+TGG 0.536172 4.1:-87500797 MS.gene054563:CDS
ACATTGAAAGCATTTAGTGT+TGG 0.540142 4.1:-87500722 MS.gene054563:CDS
AAGCGTGTTAAGGATCCAAA+TGG 0.542262 4.1:-87500557 MS.gene054563:CDS
TGCCTTCATTGAGTCGAGAT+GGG 0.557467 4.1:+87500390 None:intergenic
GAAAGTTCTCTTATTGTTTG+GGG 0.557774 4.1:-87500862 MS.gene054563:CDS
TATGGTTTGGTACAATCAGT+AGG 0.559170 4.1:+87500296 None:intergenic
ACGGTTGATGGAACGACGCA+TGG 0.561122 4.1:+87501405 None:intergenic
ACCATATAACTTAAATGGCG+GGG 0.576438 4.1:+87501288 None:intergenic
ACCGTTAGTGGGGATCACAT+TGG 0.582419 4.1:+87501339 None:intergenic
CAATTGATTTATAAGAGACA+CGG 0.582532 4.1:+87500901 None:intergenic
ATACCATATAACTTAAATGG+CGG 0.584724 4.1:+87501286 None:intergenic
TTGATGTAACAGAATAACAA+AGG 0.586188 4.1:+87500525 None:intergenic
ATAAGAAACTCACCGTTAGT+GGG 0.587177 4.1:+87501328 None:intergenic
TGGTTGTAACAGTGTTCACA+AGG 0.587638 4.1:+87501223 None:intergenic
CTCGTCGAGTTAGACGACTC+TGG 0.588889 4.1:+87500839 None:intergenic
TCCAATGTGATCCCCACTAA+CGG 0.595512 4.1:-87501340 MS.gene054563:CDS
CAATTGGGTTCATCAATTGG+TGG 0.596478 4.1:-87500884 MS.gene054563:CDS
GAGACACGGGTCCATTTCCA+AGG 0.606926 4.1:+87500915 None:intergenic
TGAAATAGAAGAACCAGAGG+TGG 0.608293 4.1:+87501012 None:intergenic
TCATAATTTGGAATTAGCTG+TGG 0.617309 4.1:-87500597 MS.gene054563:CDS
TTTCTTATGACCTATTCAGT+CGG 0.625475 4.1:-87501313 MS.gene054563:CDS
AAATTATGATAAATACCAGA+TGG 0.639824 4.1:+87500611 None:intergenic
AATTGATTTATAAGAGACAC+GGG 0.646628 4.1:+87500902 None:intergenic
TAAGAAACTCACCGTTAGTG+GGG 0.653160 4.1:+87501329 None:intergenic
TGAAGGCAAGAGCTCTGGTG+TGG 0.663721 4.1:-87500942 MS.gene054563:CDS
TAGTGTTGATGGTAACATCA+TGG 0.667211 4.1:-87500663 MS.gene054563:CDS
AAATGAAATAGAAGAACCAG+AGG 0.674192 4.1:+87501009 None:intergenic
GTTTGTAGTAATGATCGACA+CGG 0.708983 4.1:+87501386 None:intergenic
GATGATAGATCAATATCACA+AGG 0.717243 4.1:-87501067 MS.gene054563:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTAAGTAAAAAATTTGT+AGG + chr4.1:87500954-87500973 None:intergenic 10.0%
!! AAATTATGATAAATACCAGA+TGG + chr4.1:87501087-87501106 None:intergenic 20.0%
!! ACAATACCATATAACTTAAA+TGG + chr4.1:87500415-87500434 None:intergenic 20.0%
!! ATCTGGTATTTATCATAATT+TGG - chr4.1:87501086-87501105 MS.gene054563:CDS 20.0%
!!! AAAAATACAATGTCATTTGA+AGG - chr4.1:87500736-87500755 MS.gene054563:CDS 20.0%
!!! AGTTTTTTTTTTACTACAAG+AGG + chr4.1:87500587-87500606 None:intergenic 20.0%
! ATACCATATAACTTAAATGG+CGG + chr4.1:87500412-87500431 None:intergenic 25.0%
! CAAAGGTTTAAATAACCATT+TGG + chr4.1:87501156-87501175 None:intergenic 25.0%
! GATTTCAAAGGATTAAAGAT+AGG + chr4.1:87500505-87500524 None:intergenic 25.0%
! GTGTCTCTTATAAATCAATT+GGG - chr4.1:87500796-87500815 MS.gene054563:CDS 25.0%
! TTAAAATGTGCAGTGATTAT+AGG + chr4.1:87501210-87501229 None:intergenic 25.0%
! TTATATGGTATTGTTGATAC+AGG - chr4.1:87500421-87500440 MS.gene054563:CDS 25.0%
! TTGATGTAACAGAATAACAA+AGG + chr4.1:87501173-87501192 None:intergenic 25.0%
!! AATTGATTTATAAGAGACAC+GGG + chr4.1:87500796-87500815 None:intergenic 25.0%
!! ATAATCACTGCACATTTTAA+AGG - chr4.1:87501210-87501229 MS.gene054563:CDS 25.0%
!! CAATTGATTTATAAGAGACA+CGG + chr4.1:87500797-87500816 None:intergenic 25.0%
!! TTGTAACTTGATGATTTCAA+AGG + chr4.1:87500517-87500536 None:intergenic 25.0%
AAATGAAATAGAAGAACCAG+AGG + chr4.1:87500689-87500708 None:intergenic 30.0%
AATCAATTGGGTTCATCAAT+TGG - chr4.1:87500808-87500827 MS.gene054563:CDS 30.0%
CGTGTCTCTTATAAATCAAT+TGG - chr4.1:87500795-87500814 MS.gene054563:CDS 30.0%
GAAAGTTCTCTTATTGTTTG+GGG - chr4.1:87500833-87500852 MS.gene054563:CDS 30.0%
GAATTTGCAAAGCAATCAAA+TGG - chr4.1:87501009-87501028 MS.gene054563:CDS 30.0%
GATGATAGATCAATATCACA+AGG - chr4.1:87500628-87500647 MS.gene054563:CDS 30.0%
GGAAAGTTCTCTTATTGTTT+GGG - chr4.1:87500832-87500851 MS.gene054563:CDS 30.0%
TACCATATAACTTAAATGGC+GGG + chr4.1:87500411-87500430 None:intergenic 30.0%
TCATTTCCTAAAACTGTGAT+AGG - chr4.1:87500703-87500722 MS.gene054563:CDS 30.0%
TGGAAAGTTCTCTTATTGTT+TGG - chr4.1:87500831-87500850 MS.gene054563:CDS 30.0%
TTTCTTATGACCTATTCAGT+CGG - chr4.1:87500382-87500401 MS.gene054563:CDS 30.0%
! TCATAATTTGGAATTAGCTG+TGG - chr4.1:87501098-87501117 MS.gene054563:CDS 30.0%
! TTTGGCTCAACAAAATTTGT+TGG - chr4.1:87501338-87501357 MS.gene054563:CDS 30.0%
!! ACATTGAAAGCATTTAGTGT+TGG - chr4.1:87500973-87500992 MS.gene054563:CDS 30.0%
!!! TGTAGGTCTTTTTTCACTAA+AGG + chr4.1:87500937-87500956 None:intergenic 30.0%
ACCATATAACTTAAATGGCG+GGG + chr4.1:87500410-87500429 None:intergenic 35.0%
ATAAGAAACTCACCGTTAGT+GGG + chr4.1:87500370-87500389 None:intergenic 35.0%
ATCACAAGGAGATCTTAGTT+TGG - chr4.1:87500642-87500661 MS.gene054563:CDS 35.0%
GATTAAAGATAGGAGTCGTT+TGG + chr4.1:87500495-87500514 None:intergenic 35.0%
GTTTGTAGTAATGATCGACA+CGG + chr4.1:87500312-87500331 None:intergenic 35.0%
! CAATCAAATGGTAGTGTTGA+TGG - chr4.1:87501021-87501040 MS.gene054563:CDS 35.0%
! CCAAAACCTATCACAGTTTT+AGG + chr4.1:87500712-87500731 None:intergenic 35.0%
! CCTAAAACTGTGATAGGTTT+TGG - chr4.1:87500709-87500728 MS.gene054563:CDS 35.0%
!! GCTCAACAAAATTTGTTGGT+TGG - chr4.1:87501342-87501361 MS.gene054563:CDS 35.0%
!! TAGTGTTGATGGTAACATCA+TGG - chr4.1:87501032-87501051 MS.gene054563:CDS 35.0%
!! TATGGTTTGGTACAATCAGT+AGG + chr4.1:87501402-87501421 None:intergenic 35.0%
AAGCGTGTTAAGGATCCAAA+TGG - chr4.1:87501138-87501157 MS.gene054563:CDS 40.0%
ACATTGACAACTGTACCATC+TGG - chr4.1:87501069-87501088 MS.gene054563:CDS 40.0%
ATACAGGTAGCGACATAGTT+TGG - chr4.1:87500437-87500456 MS.gene054563:CDS 40.0%
CAATTGGGTTCATCAATTGG+TGG - chr4.1:87500811-87500830 MS.gene054563:CDS 40.0%
CATAAGAAACTCACCGTTAG+TGG + chr4.1:87500371-87500390 None:intergenic 40.0%
CTTACATTAGATTCCACCTC+TGG - chr4.1:87500670-87500689 MS.gene054563:CDS 40.0%
GGAATTAGCTGTGGTAGAAT+CGG - chr4.1:87501107-87501126 MS.gene054563:CDS 40.0%
GGTTAGACTAAAGCGTGTTA+AGG - chr4.1:87501128-87501147 MS.gene054563:CDS 40.0%
TAAGAAACTCACCGTTAGTG+GGG + chr4.1:87500369-87500388 None:intergenic 40.0%
TGAAATAGAAGAACCAGAGG+TGG + chr4.1:87500686-87500705 None:intergenic 40.0%
TGGATTTGGGTGTGTTAGTA+AGG + chr4.1:87500339-87500358 None:intergenic 40.0%
TGGTTGTAACAGTGTTCACA+AGG + chr4.1:87500475-87500494 None:intergenic 40.0%
! ACCCCGCCATTTAAGTTATA+TGG - chr4.1:87500406-87500425 MS.gene054563:CDS 40.0%
! ACCTTTGTTTCAGTTGCTGA+TGG - chr4.1:87501261-87501280 MS.gene054563:CDS 40.0%
! CCTTTGTTTCAGTTGCTGAT+GGG - chr4.1:87501262-87501281 MS.gene054563:CDS 40.0%
! CTTTGTTTCAGTTGCTGATG+GGG - chr4.1:87501263-87501282 MS.gene054563:CDS 40.0%
! GGTAACATCATGGTTGATTC+AGG - chr4.1:87501042-87501061 MS.gene054563:CDS 40.0%
! TCGACGAGCAAACTCAATTT+TGG - chr4.1:87500871-87500890 MS.gene054563:CDS 40.0%
!! GACTCAATGAAGGCATCTTT+GGG - chr4.1:87501313-87501332 MS.gene054563:CDS 40.0%
!! TGAAGGCATCTTTGGGAATT+TGG - chr4.1:87501320-87501339 MS.gene054563:CDS 40.0%
AGTGGGGATCACATTGGATT+TGG + chr4.1:87500353-87500372 None:intergenic 45.0%
ATGCCTTCATTGAGTCGAGA+TGG + chr4.1:87501309-87501328 None:intergenic 45.0%
CCCATCAGCAACTGAAACAA+AGG + chr4.1:87501265-87501284 None:intergenic 45.0%
GTAATGATCGACACGGTTGA+TGG + chr4.1:87500305-87500324 None:intergenic 45.0%
GTGGGGATCACATTGGATTT+GGG + chr4.1:87500352-87500371 None:intergenic 45.0%
TCATTTGAAGGCAAGAGCTC+TGG - chr4.1:87500748-87500767 MS.gene054563:CDS 45.0%
TCCAATGTGATCCCCACTAA+CGG - chr4.1:87500355-87500374 MS.gene054563:CDS 45.0%
TGCCTTCATTGAGTCGAGAT+GGG + chr4.1:87501308-87501327 None:intergenic 45.0%
!! CGACTCAATGAAGGCATCTT+TGG - chr4.1:87501312-87501331 MS.gene054563:CDS 45.0%
ACCGTTAGTGGGGATCACAT+TGG + chr4.1:87500359-87500378 None:intergenic 50.0%
GGAGAAGCTGCTGTAGTTTC+TGG - chr4.1:87500892-87500911 MS.gene054563:CDS 50.0%
GTCCCATCTCGACTCAATGA+AGG - chr4.1:87501303-87501322 MS.gene054563:CDS 50.0%
TGCTGATGGGGTTGTCTGTT+TGG - chr4.1:87501275-87501294 MS.gene054563:CDS 50.0%
!! GCTGCTGTAGTTTCTGGTCA+TGG - chr4.1:87500898-87500917 MS.gene054563:CDS 50.0%
CTCGTCGAGTTAGACGACTC+TGG + chr4.1:87500859-87500878 None:intergenic 55.0%
GAGACACGGGTCCATTTCCA+AGG + chr4.1:87500783-87500802 None:intergenic 55.0%
! TGAAGGCAAGAGCTCTGGTG+TGG - chr4.1:87500753-87500772 MS.gene054563:CDS 55.0%
!! GGTGTGGTTGGCCTTGGAAA+TGG - chr4.1:87500769-87500788 MS.gene054563:CDS 55.0%
! GGCAAGAGCTCTGGTGTGGT+TGG - chr4.1:87500757-87500776 MS.gene054563:CDS 60.0%
!! AGCTCTGGTGTGGTTGGCCT+TGG - chr4.1:87500763-87500782 MS.gene054563:CDS 60.0%
!! TGGCGGGGTACCGACTGAAT+AGG + chr4.1:87500395-87500414 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 87500292 87501425 87500292 ID=MS.gene054563
chr4.1 mRNA 87500292 87501425 87500292 ID=MS.gene054563.t1;Parent=MS.gene054563
chr4.1 exon 87500292 87501425 87500292 ID=MS.gene054563.t1.exon1;Parent=MS.gene054563.t1
chr4.1 CDS 87500292 87501425 87500292 ID=cds.MS.gene054563.t1;Parent=MS.gene054563.t1
Gene Sequence

>MS.gene054563

ATGCGTCGTTCCATCAACCGTGTCGATCATTACTACAAACATTTCCTTACTAACACACCCAAATCCAATGTGATCCCCACTAACGGTGAGTTTCTTATGACCTATTCAGTCGGTACCCCGCCATTTAAGTTATATGGTATTGTTGATACAGGTAGCGACATAGTTTGGCTTCAATCTAAGCCTTGTGAACACTGTTACAACCAAACGACTCCTATCTTTAATCCTTTGAAATCATCAAGTTACAAAAACAATATTGCTTGTTTGTCTAAAAAATGTAAGTCTTTTGAATCTACCTCTTGTAGTAAAAAAAAAACTTGCGAATATACTATTATATATGATGATAGATCAATATCACAAGGAGATCTTAGTTTGGAAACTCTTACATTAGATTCCACCTCTGGTTCTTCTATTTCATTTCCTAAAACTGTGATAGGTTTTGGACATAAAAATACAATGTCATTTGAAGGCAAGAGCTCTGGTGTGGTTGGCCTTGGAAATGGACCCGTGTCTCTTATAAATCAATTGGGTTCATCAATTGGTGGAAAGTTCTCTTATTGTTTGGGGCCAGAGTCGTCTAACTCGACGAGCAAACTCAATTTTGGAGAAGCTGCTGTAGTTTCTGGTCATGGAGTTGTGTCAACTCCTTTAGTGAAAAAAGACCTACAAATTTTTTACTTAATAACATTGAAAGCATTTAGTGTTGGAAATAAAAGAGTAGAATTTGCAAAGCAATCAAATGGTAGTGTTGATGGTAACATCATGGTTGATTCAGGTACAACATTGACAACTGTACCATCTGGTATTTATCATAATTTGGAATTAGCTGTGGTAGAATCGGTTAGACTAAAGCGTGTTAAGGATCCAAATGGTTATTTAAACCTTTGTTATTCTGTTACATCAAAAAAATATGATTTTCCTATAATCACTGCACATTTTAAAGGTGCAGATGTTAAGTTGCATTCTATTAGCACCTTTGTTTCAGTTGCTGATGGGGTTGTCTGTTTGGCTCTTGTCCCATCTCGACTCAATGAAGGCATCTTTGGGAATTTGGCTCAACAAAATTTGTTGGTTGGTTATGATAGTCAACAAAAAATCATGTCTTTTAAGCCTACTGATTGTACCAAACCATATTGA
Protein sequence

>MS.gene054563.t1

MRRSINRVDHYYKHFLTNTPKSNVIPTNGEFLMTYSVGTPPFKLYGIVDTGSDIVWLQSKPCEHCYNQTTPIFNPLKSSSYKNNIACLSKKCKSFESTSCSKKKTCEYTIIYDDRSISQGDLSLETLTLDSTSGSSISFPKTVIGFGHKNTMSFEGKSSGVVGLGNGPVSLINQLGSSIGGKFSYCLGPESSNSTSKLNFGEAAVVSGHGVVSTPLVKKDLQIFYLITLKAFSVGNKRVEFAKQSNGSVDGNIMVDSGTTLTTVPSGIYHNLELAVVESVRLKRVKDPNGYLNLCYSVTSKKYDFPIITAHFKGADVKLHSISTFVSVADGVVCLALVPSRLNEGIFGNLAQQNLLVGYDSQQKIMSFKPTDCTKPY