AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054572

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054572.t1 MTR_8g096990 66.578 377 121 4 2 376 58 431 8.94e-180 506
MS.gene054572.t1 MTR_5g080960 66.755 379 119 5 2 376 58 433 4.11e-176 497
MS.gene054572.t1 MTR_5g044770 66.578 377 120 5 2 376 58 430 2.68e-174 492
MS.gene054572.t1 MTR_5g080890 66.227 379 121 5 2 376 58 433 6.91e-174 491
MS.gene054572.t1 MTR_8g038280 65.092 381 124 4 2 376 58 435 5.78e-173 489
MS.gene054572.t1 MTR_8g038250 63.874 382 107 5 2 376 58 415 2.64e-166 471
MS.gene054572.t1 MTR_8g038310 58.466 378 137 4 1 378 58 415 2.46e-156 446
MS.gene054572.t1 MTR_6g090290 60.677 384 139 6 1 376 57 436 1.03e-146 422
MS.gene054572.t1 MTR_4g109760 61.376 378 116 5 2 376 48 398 7.51e-146 419
MS.gene054572.t1 MTR_3g028120 51.862 376 173 6 7 376 65 438 1.44e-127 374
MS.gene054572.t1 MTR_2g067830 54.286 385 163 11 1 376 26 406 3.11e-123 362
MS.gene054572.t1 MTR_5g038360 48.320 387 184 10 1 376 55 436 9.20e-114 338
MS.gene054572.t1 MTR_0024s0040 48.421 380 178 9 10 376 36 410 4.30e-109 326
MS.gene054572.t1 MTR_2g105300 50.524 382 154 12 1 373 26 381 6.64e-109 324
MS.gene054572.t1 MTR_6g088760 51.977 354 155 8 3 345 59 408 1.14e-108 328
MS.gene054572.t1 MTR_5g044570 45.263 380 170 10 3 376 64 411 2.01e-100 303
MS.gene054572.t1 MTR_2g059490 45.634 355 181 10 30 375 58 409 1.23e-93 287
MS.gene054572.t1 MTR_5g008070 43.869 367 197 8 13 375 64 425 2.10e-93 286
MS.gene054572.t1 MTR_2g059460 42.969 384 199 13 1 376 35 406 3.96e-93 285
MS.gene054572.t1 MTR_0036s0050 41.333 375 201 10 11 376 71 435 3.72e-87 270
MS.gene054572.t1 MTR_5g008110 41.573 356 194 8 29 376 66 415 5.16e-87 269
MS.gene054572.t1 MTR_0036s0070 40.431 371 201 10 11 376 71 426 5.79e-87 270
MS.gene054572.t1 MTR_5g008150 39.948 383 215 9 3 376 36 412 8.24e-86 266
MS.gene054572.t1 MTR_0036s0110 38.802 384 206 11 12 376 69 442 2.29e-82 258
MS.gene054572.t1 MTR_1g060750 35.200 375 206 11 12 376 29 376 5.24e-65 211
MS.gene054572.t1 MTR_3g097150 35.813 363 219 9 21 376 93 448 7.68e-64 210
MS.gene054572.t1 MTR_5g008090 39.333 300 166 10 87 376 21 314 5.38e-59 194
MS.gene054572.t1 MTR_4g045857 44.262 244 128 6 18 254 46 288 8.02e-58 190
MS.gene054572.t1 MTR_7g117430 35.014 357 209 13 29 374 140 484 4.25e-51 178
MS.gene054572.t1 MTR_6g088765 43.277 238 102 5 105 341 3 208 1.39e-49 167
MS.gene054572.t1 MTR_1g039270 33.239 355 216 11 29 374 127 469 1.81e-49 173
MS.gene054572.t1 MTR_4g008110 77.876 113 4 1 104 216 46 137 7.96e-49 162
MS.gene054572.t1 MTR_1g115210 33.521 355 214 11 29 374 145 486 9.84e-48 169
MS.gene054572.t1 MTR_1g111350 34.463 354 208 13 29 374 130 467 3.53e-46 164
MS.gene054572.t1 MTR_2g028190 32.432 370 223 14 21 374 93 451 1.73e-40 149
MS.gene054572.t1 MTR_3g046300 33.077 390 228 14 12 376 79 460 7.50e-40 147
MS.gene054572.t1 MTR_6g085010 32.286 350 218 10 30 374 156 491 3.47e-39 146
MS.gene054572.t1 MTR_4g007740 31.594 345 207 14 47 375 147 478 5.67e-38 142
MS.gene054572.t1 MTR_3g053710 33.884 363 211 15 29 374 116 466 6.17e-38 142
MS.gene054572.t1 MTR_5g091130 31.105 389 236 15 12 375 173 554 2.85e-36 139
MS.gene054572.t1 MTR_8g103880 29.870 385 233 17 7 374 91 455 5.58e-31 123
MS.gene054572.t1 MTR_3g068000 28.836 378 221 14 33 374 76 441 6.13e-31 123
MS.gene054572.t1 MTR_3g065160 28.075 374 221 16 31 376 75 428 4.93e-27 111
MS.gene054572.t1 MTR_7g105850 30.374 428 216 22 10 376 61 467 9.39e-27 111
MS.gene054572.t1 MTR_2g070430 29.396 364 221 12 37 371 94 450 1.22e-26 110
MS.gene054572.t1 MTR_2g055840 26.984 378 242 13 22 374 5 373 5.19e-26 107
MS.gene054572.t1 MTR_7g080230 27.632 380 228 17 27 375 68 431 2.88e-25 107
MS.gene054572.t1 MTR_0945s0010 40.000 125 71 4 253 375 1 123 2.49e-24 98.2
MS.gene054572.t1 MTR_3g053980 29.539 369 236 14 18 374 129 485 3.00e-24 104
MS.gene054572.t1 MTR_7g021510 26.963 382 226 18 29 375 71 434 6.55e-24 103
MS.gene054572.t1 MTR_7g062360 26.984 378 226 15 33 374 62 425 1.08e-23 102
MS.gene054572.t1 MTR_8g094590 26.146 371 231 14 29 374 72 424 2.22e-23 101
MS.gene054572.t1 MTR_5g077850 26.260 377 228 15 31 376 82 439 4.82e-23 100
MS.gene054572.t1 MTR_1g078240 26.355 406 250 20 5 375 39 430 5.63e-22 97.4
MS.gene054572.t1 MTR_1g029810 25.067 375 235 14 29 376 56 411 5.84e-22 97.1
MS.gene054572.t1 MTR_1g029810 25.067 375 235 14 29 376 56 411 6.98e-22 97.1
MS.gene054572.t1 MTR_3g096930 25.000 388 237 16 22 376 70 436 1.43e-21 95.9
MS.gene054572.t1 MTR_1g078240 26.961 408 246 21 5 375 39 431 2.34e-21 95.5
MS.gene054572.t1 MTR_6g084710 26.649 379 233 15 29 377 190 553 4.01e-21 95.5
MS.gene054572.t1 MTR_4g023650 25.441 397 249 16 10 376 42 421 5.70e-21 94.0
MS.gene054572.t1 MTR_8g094590 28.777 278 171 10 29 297 72 331 1.13e-20 93.2
MS.gene054572.t1 MTR_4g095270 26.287 369 229 16 29 376 79 425 1.33e-20 94.0
MS.gene054572.t1 MTR_4g095270 26.287 369 229 16 29 376 79 425 1.37e-20 94.0
MS.gene054572.t1 MTR_5g016260 25.405 370 231 16 29 376 87 433 1.38e-20 94.0
MS.gene054572.t1 MTR_5g016260 25.405 370 231 16 29 376 87 433 1.84e-20 93.6
MS.gene054572.t1 MTR_4g100990 27.361 413 216 20 30 374 74 470 4.47e-20 92.0
MS.gene054572.t1 MTR_8g094590 29.297 256 155 9 29 276 72 309 4.99e-20 90.1
MS.gene054572.t1 MTR_4g056420 27.467 375 231 16 29 375 76 437 6.83e-20 91.3
MS.gene054572.t1 MTR_5g012490 25.980 408 227 18 30 374 74 469 1.56e-19 90.1
MS.gene054572.t1 MTR_7g080230 27.479 353 209 17 54 375 25 361 2.22e-19 89.4
MS.gene054572.t1 MTR_4g023630 25.441 397 249 16 10 376 42 421 2.62e-19 89.4
MS.gene054572.t1 MTR_3g014290 29.050 358 220 15 31 375 81 417 3.97e-19 88.6
MS.gene054572.t1 MTR_2g084550 28.796 382 218 18 29 375 76 438 5.56e-19 88.6
MS.gene054572.t1 MTR_1g078240 27.941 340 203 18 5 316 39 364 6.76e-19 87.8
MS.gene054572.t1 MTR_1g037630 28.852 357 222 14 31 375 93 429 7.41e-19 87.8
MS.gene054572.t1 MTR_5g077920 24.934 381 230 13 31 376 69 428 1.88e-18 87.0
MS.gene054572.t1 MTR_6g084710 26.140 329 210 13 29 339 190 503 2.76e-18 86.7
MS.gene054572.t1 MTR_5g080330 26.816 358 195 17 47 374 2 322 2.74e-17 83.2
MS.gene054572.t1 MTR_1g061940 25.936 374 240 18 29 375 82 445 2.21e-16 80.9
MS.gene054572.t1 MTR_8g039540 29.167 360 218 17 31 375 97 434 4.11e-16 79.7
MS.gene054572.t1 MTR_7g102750 25.269 372 243 16 29 375 80 441 1.03e-15 79.0
MS.gene054572.t1 MTR_2g063110 24.138 406 234 17 29 374 117 508 1.62e-15 78.2
MS.gene054572.t1 MTR_7g102750 26.623 308 196 14 29 316 80 377 5.24e-14 73.6
MS.gene054572.t1 MTR_3g100500 28.612 353 208 16 29 361 96 424 1.13e-13 72.4
MS.gene054572.t1 MTR_8g075090 26.087 368 221 20 36 376 118 461 1.64e-13 72.0
MS.gene054572.t1 MTR_8g075090 25.956 366 220 20 36 374 118 459 9.48e-13 69.7
MS.gene054572.t1 MTR_3g006820 22.506 391 259 15 15 376 39 414 1.07e-11 66.2
MS.gene054572.t1 MTR_2g103340 69.231 39 12 0 42 80 67 105 1.07e-11 63.9
MS.gene054572.t1 MTR_5g061690 26.689 296 175 16 84 362 24 294 1.33e-11 65.9
MS.gene054572.t1 MTR_3g087780 24.868 378 211 20 37 374 106 450 4.02e-11 64.7
MS.gene054572.t1 MTR_8g075090 25.836 329 202 17 36 341 118 427 4.84e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054572.t1 AT1G64830 45.000 380 201 7 1 376 55 430 1.89e-107 322
MS.gene054572.t1 AT5G33340 47.769 381 190 8 1 376 60 436 2.02e-107 322
MS.gene054572.t1 AT2G35615 45.431 383 187 10 12 375 65 444 5.34e-98 298
MS.gene054572.t1 AT1G31450 42.857 371 193 9 21 375 75 442 5.75e-85 265
MS.gene054572.t1 AT2G28010 43.503 354 171 13 31 378 65 395 5.28e-80 251
MS.gene054572.t1 AT2G28040 42.535 355 168 13 30 375 64 391 1.52e-76 242
MS.gene054572.t1 AT2G28040 42.535 355 168 13 30 375 55 382 1.83e-76 241
MS.gene054572.t1 AT2G28030 43.220 354 172 13 31 378 61 391 2.36e-76 241
MS.gene054572.t1 AT2G28220 41.808 354 175 13 31 375 67 398 4.72e-71 228
MS.gene054572.t1 AT2G28225 39.718 355 183 13 31 376 67 399 9.95e-66 214
MS.gene054572.t1 AT2G03200 37.731 379 206 14 17 376 93 460 5.51e-58 196
MS.gene054572.t1 AT1G01300 36.415 357 204 13 29 374 140 484 4.75e-53 183
MS.gene054572.t1 AT3G61820 34.986 363 207 13 29 374 133 483 1.40e-47 168
MS.gene054572.t1 AT3G18490 32.800 375 227 14 10 374 141 500 2.43e-46 166
MS.gene054572.t1 AT1G25510 32.955 352 216 10 29 374 146 483 8.16e-44 158
MS.gene054572.t1 AT3G20015 32.493 357 215 14 29 374 129 470 2.47e-42 154
MS.gene054572.t1 AT2G23945 30.328 366 224 12 31 376 96 450 1.00e-41 152
MS.gene054572.t1 AT4G30030 32.143 364 221 15 23 374 70 419 1.09e-41 151
MS.gene054572.t1 AT5G10770 35.733 375 206 16 21 375 115 474 7.35e-41 150
MS.gene054572.t1 AT5G10760 33.504 391 228 15 1 374 89 464 8.15e-40 147
MS.gene054572.t1 AT1G79720 34.501 371 193 19 31 374 135 482 5.56e-38 142
MS.gene054572.t1 AT3G12700 30.334 389 241 14 13 375 77 461 8.72e-36 136
MS.gene054572.t1 AT3G25700 30.159 378 228 16 29 377 82 452 2.26e-35 135
MS.gene054572.t1 AT4G30040 31.818 374 211 20 19 369 75 427 8.99e-35 133
MS.gene054572.t1 AT2G42980 30.628 382 240 13 16 374 145 524 2.08e-34 133
MS.gene054572.t1 AT3G59080 29.737 380 244 14 16 374 155 532 9.15e-33 129
MS.gene054572.t1 AT2G39710 29.412 374 212 16 33 369 67 425 2.28e-32 126
MS.gene054572.t1 AT5G36260 27.249 378 225 16 29 375 76 434 9.94e-31 122
MS.gene054572.t1 AT5G02190 27.151 372 223 16 40 374 82 442 4.12e-28 115
MS.gene054572.t1 AT1G09750 27.820 399 246 15 1 374 67 448 1.86e-25 107
MS.gene054572.t1 AT1G66180 25.789 380 225 16 31 376 72 428 2.01e-25 107
MS.gene054572.t1 AT3G59080 27.467 375 223 12 16 374 155 496 2.47e-25 107
MS.gene054572.t1 AT5G37540 27.200 375 226 16 31 374 80 438 2.94e-24 103
MS.gene054572.t1 AT5G45120 27.119 413 215 19 31 374 83 478 3.03e-24 104
MS.gene054572.t1 AT3G54400 28.974 390 237 17 6 375 56 425 3.66e-24 103
MS.gene054572.t1 AT3G02740 26.902 368 238 16 29 375 83 440 6.82e-24 103
MS.gene054572.t1 AT3G02740 27.222 360 231 16 37 375 16 365 1.10e-23 102
MS.gene054572.t1 AT4G33490 26.434 401 237 18 12 376 35 413 3.37e-23 100
MS.gene054572.t1 AT1G65240 26.596 376 227 16 29 375 72 427 8.60e-23 100
MS.gene054572.t1 AT1G44130 27.068 399 236 18 12 376 24 401 1.46e-22 98.6
MS.gene054572.t1 AT5G43100 27.248 367 229 14 27 375 72 418 1.63e-22 99.0
MS.gene054572.t1 AT5G43100 27.248 367 229 14 27 375 72 418 4.47e-22 98.6
MS.gene054572.t1 AT1G49050 24.157 356 238 12 48 377 49 398 2.90e-20 92.0
MS.gene054572.t1 AT2G17760 27.198 364 217 18 36 374 109 449 5.53e-20 91.7
MS.gene054572.t1 AT1G49050 24.377 361 231 13 48 377 222 571 7.05e-20 91.7
MS.gene054572.t1 AT5G22850 27.851 377 228 17 29 375 79 441 7.71e-20 91.3
MS.gene054572.t1 AT4G33490 27.703 296 174 13 12 287 32 307 5.86e-19 88.2
MS.gene054572.t1 AT1G05840 25.789 380 231 15 29 375 78 439 7.26e-19 88.2
MS.gene054572.t1 AT3G50050 25.410 366 237 14 29 376 91 438 5.94e-18 85.9
MS.gene054572.t1 AT1G08210 26.203 374 235 17 29 375 116 475 3.94e-17 83.2
MS.gene054572.t1 AT2G36670 26.216 370 241 17 29 375 98 458 4.03e-17 83.2
MS.gene054572.t1 AT1G08210 26.203 374 235 17 29 375 130 489 4.24e-17 83.2
MS.gene054572.t1 AT1G08210 26.203 374 235 17 29 375 82 441 4.50e-17 83.2
MS.gene054572.t1 AT2G36670 26.519 362 234 17 37 375 111 463 8.09e-17 82.4
MS.gene054572.t1 AT2G36670 26.446 363 233 18 37 375 40 392 1.06e-16 81.6
MS.gene054572.t1 AT2G36670 26.446 363 233 18 37 375 40 392 1.06e-16 81.6
MS.gene054572.t1 AT1G49050 26.800 250 161 9 48 285 222 461 2.40e-16 80.9
MS.gene054572.t1 AT5G07030 28.448 348 213 15 31 362 115 442 5.23e-16 79.7
MS.gene054572.t1 AT3G42550 22.642 371 223 12 21 375 68 390 6.11e-16 79.3
MS.gene054572.t1 AT1G08210 27.832 309 187 15 29 315 82 376 3.09e-14 74.3
MS.gene054572.t1 AT3G51340 25.414 362 225 18 36 374 108 447 1.48e-12 69.3
MS.gene054572.t1 AT5G24820 25.979 281 184 10 111 378 134 403 1.64e-12 68.9
MS.gene054572.t1 AT3G25700 30.709 127 79 4 29 147 82 207 3.23e-12 67.4

Find 82 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAAATGTGCAGTGATTA+TGG 0.131217 4.1:+87442296 None:intergenic
ATCTGGTATTTATCATAATT+TGG 0.150549 4.1:-87442421 MS.gene054572:CDS
GGAGAAGCTGCTATAGTTTC+TGG 0.180325 4.1:-87442615 MS.gene054572:CDS
TCAACGAGCAAACTCAATTT+TGG 0.205188 4.1:-87442636 MS.gene054572:CDS
GGAAAGTTCTCTTATTGTTT+GGG 0.226977 4.1:-87442675 MS.gene054572:CDS
AGCGGGCATCACAGTGGATT+TGG 0.236368 4.1:+87443157 None:intergenic
ACCCCACCATTTAAGTTATA+TGG 0.251212 4.1:-87443101 MS.gene054572:CDS
CCTAAAACCGTGATAGGTTT+TGG 0.252734 4.1:-87442798 MS.gene054572:CDS
CTACTCAATAAAGGCATCTT+TGG 0.257937 4.1:-87442192 MS.gene054572:CDS
TGGAAAGTTCTCTTATTGTT+TGG 0.261051 4.1:-87442676 MS.gene054572:CDS
ACAATACCATATAACTTAAA+TGG 0.274831 4.1:+87443095 None:intergenic
TCATTTCCTAAAACCGTGAT+AGG 0.315869 4.1:-87442804 MS.gene054572:CDS
CGTGTCTCTAATAAATCAAT+TGG 0.324779 4.1:-87442712 MS.gene054572:CDS
AATCAATTGGGTTCATCAAT+TGG 0.334833 4.1:-87442699 MS.gene054572:CDS
TGGATTTGGGTGTGTTAGTA+AGG 0.346495 4.1:+87443171 None:intergenic
AACAAAGTCTACACTCAATA+TGG 0.354389 4.1:+87442087 None:intergenic
GGTAACATCATAGTTGATTC+AGG 0.362416 4.1:-87442465 MS.gene054572:CDS
GCGGGCATCACAGTGGATTT+GGG 0.367396 4.1:+87443158 None:intergenic
GTCCGATCTCTACTCAATAA+AGG 0.384414 4.1:-87442201 MS.gene054572:CDS
GGTGTGGTTGGCCTTGGAAA+TGG 0.390490 4.1:-87442738 MS.gene054572:CDS
TGCGAATATAGTATTAAGTA+TGG 0.394209 4.1:-87442900 MS.gene054572:CDS
TCAAAATGTGCAGTGATTAT+GGG 0.395459 4.1:+87442297 None:intergenic
CAATCAAATGGTAGTGTTGA+TGG 0.396941 4.1:-87442486 MS.gene054572:CDS
AGCTCTGGTGTGGTTGGCCT+TGG 0.398793 4.1:-87442744 MS.gene054572:CDS
AACAATACATTGTCATTTGA+AGG 0.406579 4.1:-87442771 MS.gene054572:CDS
ACCTTTGCTCGAGTTTCTGA+TGG 0.407459 4.1:-87442243 MS.gene054572:CDS
TACTCAATAAAGGCATCTTT+GGG 0.415339 4.1:-87442191 MS.gene054572:CDS
ATCACAAGGAGATCTTAGTT+TGG 0.415779 4.1:-87442865 MS.gene054572:CDS
TAAGGGAATGTTTGTAGTAA+TGG 0.419028 4.1:+87443189 None:intergenic
ATCATATTTATTTGATGCAA+CGG 0.425117 4.1:+87442323 None:intergenic
GGTATCAGCTGTGGTAAAAT+CGG 0.426564 4.1:-87442400 MS.gene054572:CDS
TCTCTTATTGTTTGGGGCCA+TGG 0.430440 4.1:-87442668 MS.gene054572:CDS
CATAAGAAACTCACCTTTAG+CGG 0.438405 4.1:+87443139 None:intergenic
TTATATGGTATTGTTGATAC+AGG 0.443331 4.1:-87443086 MS.gene054572:CDS
ATACAGGTAGTGACATGGTT+TGG 0.456250 4.1:-87443070 MS.gene054572:CDS
CCTTTGCTCGAGTTTCTGAT+GGG 0.459517 4.1:-87442242 MS.gene054572:CDS
CTTACATTAGATTCCACCTC+TGG 0.468740 4.1:-87442837 MS.gene054572:CDS
TCATTTGAAGGCAAGAGCTC+TGG 0.470790 4.1:-87442759 MS.gene054572:CDS
TGGTGGGGTACCGACTGAAT+AGG 0.480047 4.1:+87443115 None:intergenic
GGCAAGAGCTCTGGTGTGGT+TGG 0.491732 4.1:-87442750 MS.gene054572:CDS
TACCATATAACTTAAATGGT+GGG 0.492301 4.1:+87443099 None:intergenic
TCCACTGTGATGCCCGCTAA+AGG 0.499859 4.1:-87443152 MS.gene054572:CDS
AGTCTACACTCAATATGGTT+TGG 0.504081 4.1:+87442092 None:intergenic
TTGTAACTTGATGAATTCAA+AGG 0.507205 4.1:+87442993 None:intergenic
AAGCGTGCAAAGGATCCAAC+TGG 0.524991 4.1:-87442369 MS.gene054572:CDS
GTGTCTCTAATAAATCAATT+GGG 0.531292 4.1:-87442711 MS.gene054572:CDS
TATAGTATTAAGTATGGTGA+TGG 0.532681 4.1:-87442894 MS.gene054572:CDS
ACATTGAAAGCATTTAGTGT+TGG 0.540142 4.1:-87442534 MS.gene054572:CDS
GCTGCTATAGTTTCTGGTCA+TGG 0.541311 4.1:-87442609 MS.gene054572:CDS
TGCCTTTATTGAGTAGAGAT+CGG 0.542952 4.1:+87442199 None:intergenic
GCAGTGACAGTTGTACCATC+TGG 0.543688 4.1:-87442438 MS.gene054572:CDS
TCATAATTTGGTATCAGCTG+TGG 0.549938 4.1:-87442409 MS.gene054572:CDS
GCTCAACAGAATTTGTTGGT+TGG 0.552408 4.1:-87442162 MS.gene054572:CDS
GAAAGTTCTCTTATTGTTTG+GGG 0.557774 4.1:-87442674 MS.gene054572:CDS
CAATTGATTTATTAGAGACA+CGG 0.558463 4.1:+87442713 None:intergenic
GTTATGTCCAAAACCTATCA+CGG 0.558819 4.1:+87442791 None:intergenic
TATGGTTTGGTACAATCAGT+AGG 0.559170 4.1:+87442105 None:intergenic
ATACCATATAACTTAAATGG+TGG 0.565852 4.1:+87443098 None:intergenic
CTCGTTGAGTTAGACGACCA+TGG 0.566118 4.1:+87442651 None:intergenic
GAGACACGGGTCCATTTCCA+AGG 0.570796 4.1:+87442727 None:intergenic
AGATTAAGAAAACCACCAGT+TGG 0.573212 4.1:+87442354 None:intergenic
GGATTTGGGTGTGTTAGTAA+GGG 0.573804 4.1:+87443172 None:intergenic
ACCATATAACTTAAATGGTG+GGG 0.576438 4.1:+87443100 None:intergenic
ATAAGAAACTCACCTTTAGC+GGG 0.577860 4.1:+87443140 None:intergenic
TGGTTGTAACAGTGTTCACA+AGG 0.587638 4.1:+87443035 None:intergenic
CCCATCAGAAACTCGAGCAA+AGG 0.593408 4.1:+87442242 None:intergenic
CAATTGGGTTCATCAATTGG+TGG 0.596478 4.1:-87442696 MS.gene054572:CDS
AATTGATTTATTAGAGACAC+GGG 0.597238 4.1:+87442714 None:intergenic
CATCATAGTTGATTCAGGTA+CGG 0.597806 4.1:-87442460 MS.gene054572:CDS
ATGATCGATGGAACGACGCA+TGG 0.598544 4.1:+87443217 None:intergenic
CTTTGCTCGAGTTTCTGATG+GGG 0.607379 4.1:-87442241 MS.gene054572:CDS
TGAAATAGAAGAACCAGAGG+TGG 0.608293 4.1:+87442824 None:intergenic
GTAATGGTCGACATGATCGA+TGG 0.617928 4.1:+87443205 None:intergenic
TTTCTTATGACCTATTCAGT+CGG 0.625475 4.1:-87443125 MS.gene054572:CDS
TGTTGATACAGGTAGTGACA+TGG 0.630223 4.1:-87443075 MS.gene054572:CDS
GGTTAAACTGAAGCGTGCAA+AGG 0.630660 4.1:-87442379 MS.gene054572:CDS
AAATTATGATAAATACCAGA+TGG 0.652242 4.1:+87442423 None:intergenic
TGAAGGCAAGAGCTCTGGTG+TGG 0.663721 4.1:-87442754 MS.gene054572:CDS
ACCTTTAGCGGGCATCACAG+TGG 0.667290 4.1:+87443151 None:intergenic
AAATGAAATAGAAGAACCAG+AGG 0.674192 4.1:+87442821 None:intergenic
CGTGCAAAGGATCCAACTGG+TGG 0.682034 4.1:-87442366 MS.gene054572:CDS
GGTGATGGATCAATATCACA+AGG 0.695175 4.1:-87442879 MS.gene054572:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTAAGTAAGCAATTTTT+GGG + chr4.1:87442763-87442782 None:intergenic 15.0%
!! AAATTATGATAAATACCAGA+TGG + chr4.1:87442896-87442915 None:intergenic 20.0%
!! ACAATACCATATAACTTAAA+TGG + chr4.1:87442224-87442243 None:intergenic 20.0%
!! ATCATATTTATTTGATGCAA+CGG + chr4.1:87442996-87443015 None:intergenic 20.0%
!! ATCTGGTATTTATCATAATT+TGG - chr4.1:87442895-87442914 MS.gene054572:CDS 20.0%
!! GAATTCAAAGGATTAAAAAT+AGG + chr4.1:87442314-87442333 None:intergenic 20.0%
!!! AGTTTTGTTTTTATTACAAG+AGG + chr4.1:87442396-87442415 None:intergenic 20.0%
!!! GTTATTAAGTAAGCAATTTT+TGG + chr4.1:87442764-87442783 None:intergenic 20.0%
!!! TTACATTTTTTAGACAAACA+AGG + chr4.1:87442359-87442378 None:intergenic 20.0%
! ATACCATATAACTTAAATGG+TGG + chr4.1:87442221-87442240 None:intergenic 25.0%
! GTGTCTCTAATAAATCAATT+GGG - chr4.1:87442605-87442624 MS.gene054572:CDS 25.0%
! TACCATATAACTTAAATGGT+GGG + chr4.1:87442220-87442239 None:intergenic 25.0%
! TATAGTATTAAGTATGGTGA+TGG - chr4.1:87442422-87442441 MS.gene054572:CDS 25.0%
! TGCGAATATAGTATTAAGTA+TGG - chr4.1:87442416-87442435 MS.gene054572:CDS 25.0%
! TTATATGGTATTGTTGATAC+AGG - chr4.1:87442230-87442249 MS.gene054572:CDS 25.0%
! TTGTAACTTGATGAATTCAA+AGG + chr4.1:87442326-87442345 None:intergenic 25.0%
!! AACAATACATTGTCATTTGA+AGG - chr4.1:87442545-87442564 MS.gene054572:CDS 25.0%
!! AATTGATTTATTAGAGACAC+GGG + chr4.1:87442605-87442624 None:intergenic 25.0%
!! CAATTGATTTATTAGAGACA+CGG + chr4.1:87442606-87442625 None:intergenic 25.0%
!! GAATTTTCAAAGCAATCAAA+TGG - chr4.1:87442818-87442837 MS.gene054572:CDS 25.0%
AAATGAAATAGAAGAACCAG+AGG + chr4.1:87442498-87442517 None:intergenic 30.0%
AATCAATTGGGTTCATCAAT+TGG - chr4.1:87442617-87442636 MS.gene054572:CDS 30.0%
ACCATATAACTTAAATGGTG+GGG + chr4.1:87442219-87442238 None:intergenic 30.0%
CGTGTCTCTAATAAATCAAT+TGG - chr4.1:87442604-87442623 MS.gene054572:CDS 30.0%
GAAAGTTCTCTTATTGTTTG+GGG - chr4.1:87442642-87442661 MS.gene054572:CDS 30.0%
GATTAAAAATAGGAGTCGTT+TGG + chr4.1:87442304-87442323 None:intergenic 30.0%
GGAAAGTTCTCTTATTGTTT+GGG - chr4.1:87442641-87442660 MS.gene054572:CDS 30.0%
TAAGGGAATGTTTGTAGTAA+TGG + chr4.1:87442130-87442149 None:intergenic 30.0%
TCAAAATGTGCAGTGATTAT+GGG + chr4.1:87443022-87443041 None:intergenic 30.0%
TGGAAAGTTCTCTTATTGTT+TGG - chr4.1:87442640-87442659 MS.gene054572:CDS 30.0%
TTCAAAATGTGCAGTGATTA+TGG + chr4.1:87443023-87443042 None:intergenic 30.0%
TTTCTTATGACCTATTCAGT+CGG - chr4.1:87442191-87442210 MS.gene054572:CDS 30.0%
! ATAATCACTGCACATTTTGA+AGG - chr4.1:87443022-87443041 MS.gene054572:CDS 30.0%
! TTTTGCTCAACAGAATTTGT+TGG - chr4.1:87443150-87443169 MS.gene054572:CDS 30.0%
!! ACATTGAAAGCATTTAGTGT+TGG - chr4.1:87442782-87442801 MS.gene054572:CDS 30.0%
!! TACTCAATAAAGGCATCTTT+GGG - chr4.1:87443125-87443144 MS.gene054572:CDS 30.0%
!!! TTTGGGTCTTTTTTCACTAA+AGG + chr4.1:87442746-87442765 None:intergenic 30.0%
AGATTAAGAAAACCACCAGT+TGG + chr4.1:87442965-87442984 None:intergenic 35.0%
ATAAGAAACTCACCTTTAGC+GGG + chr4.1:87442179-87442198 None:intergenic 35.0%
ATCACAAGGAGATCTTAGTT+TGG - chr4.1:87442451-87442470 MS.gene054572:CDS 35.0%
CATAAGAAACTCACCTTTAG+CGG + chr4.1:87442180-87442199 None:intergenic 35.0%
GTTATGTCCAAAACCTATCA+CGG + chr4.1:87442528-87442547 None:intergenic 35.0%
TCATAATTTGGTATCAGCTG+TGG - chr4.1:87442907-87442926 MS.gene054572:CDS 35.0%
TCATTTCCTAAAACCGTGAT+AGG - chr4.1:87442512-87442531 MS.gene054572:CDS 35.0%
TGCCTTTATTGAGTAGAGAT+CGG + chr4.1:87443120-87443139 None:intergenic 35.0%
! ACCCCACCATTTAAGTTATA+TGG - chr4.1:87442215-87442234 MS.gene054572:CDS 35.0%
! CAATCAAATGGTAGTGTTGA+TGG - chr4.1:87442830-87442849 MS.gene054572:CDS 35.0%
! CATCATAGTTGATTCAGGTA+CGG - chr4.1:87442856-87442875 MS.gene054572:CDS 35.0%
! GGTAACATCATAGTTGATTC+AGG - chr4.1:87442851-87442870 MS.gene054572:CDS 35.0%
! TCAACGAGCAAACTCAATTT+TGG - chr4.1:87442680-87442699 MS.gene054572:CDS 35.0%
!! CTACTCAATAAAGGCATCTT+TGG - chr4.1:87443124-87443143 MS.gene054572:CDS 35.0%
!! TATGGTTTGGTACAATCAGT+AGG + chr4.1:87443214-87443233 None:intergenic 35.0%
ATACAGGTAGTGACATGGTT+TGG - chr4.1:87442246-87442265 MS.gene054572:CDS 40.0%
CAATTGGGTTCATCAATTGG+TGG - chr4.1:87442620-87442639 MS.gene054572:CDS 40.0%
CTTACATTAGATTCCACCTC+TGG - chr4.1:87442479-87442498 MS.gene054572:CDS 40.0%
GGATTTGGGTGTGTTAGTAA+GGG + chr4.1:87442147-87442166 None:intergenic 40.0%
GGTATCAGCTGTGGTAAAAT+CGG - chr4.1:87442916-87442935 MS.gene054572:CDS 40.0%
GTCCGATCTCTACTCAATAA+AGG - chr4.1:87443115-87443134 MS.gene054572:CDS 40.0%
TGAAATAGAAGAACCAGAGG+TGG + chr4.1:87442495-87442514 None:intergenic 40.0%
TGGATTTGGGTGTGTTAGTA+AGG + chr4.1:87442148-87442167 None:intergenic 40.0%
TGGTTGTAACAGTGTTCACA+AGG + chr4.1:87442284-87442303 None:intergenic 40.0%
TGTTGATACAGGTAGTGACA+TGG - chr4.1:87442241-87442260 MS.gene054572:CDS 40.0%
! CCAAAACCTATCACGGTTTT+AGG + chr4.1:87442521-87442540 None:intergenic 40.0%
! CCTAAAACCGTGATAGGTTT+TGG - chr4.1:87442518-87442537 MS.gene054572:CDS 40.0%
!! GCTCAACAGAATTTGTTGGT+TGG - chr4.1:87443154-87443173 MS.gene054572:CDS 40.0%
!! GGTGATGGATCAATATCACA+AGG - chr4.1:87442437-87442456 MS.gene054572:CDS 40.0%
ACCTTTGCTCGAGTTTCTGA+TGG - chr4.1:87443073-87443092 MS.gene054572:CDS 45.0%
CCTTTGCTCGAGTTTCTGAT+GGG - chr4.1:87443074-87443093 MS.gene054572:CDS 45.0%
CTTTGCTCGAGTTTCTGATG+GGG - chr4.1:87443075-87443094 MS.gene054572:CDS 45.0%
GGAGAAGCTGCTATAGTTTC+TGG - chr4.1:87442701-87442720 MS.gene054572:CDS 45.0%
GGTTAAACTGAAGCGTGCAA+AGG - chr4.1:87442937-87442956 MS.gene054572:CDS 45.0%
GTAATGGTCGACATGATCGA+TGG + chr4.1:87442114-87442133 None:intergenic 45.0%
TCATTTGAAGGCAAGAGCTC+TGG - chr4.1:87442557-87442576 MS.gene054572:CDS 45.0%
! TCTCTTATTGTTTGGGGCCA+TGG - chr4.1:87442648-87442667 MS.gene054572:CDS 45.0%
!! GCTGCTATAGTTTCTGGTCA+TGG - chr4.1:87442707-87442726 MS.gene054572:CDS 45.0%
AAGCGTGCAAAGGATCCAAC+TGG - chr4.1:87442947-87442966 MS.gene054572:CDS 50.0%
CCCATCAGAAACTCGAGCAA+AGG + chr4.1:87443077-87443096 None:intergenic 50.0%
CTCGTTGAGTTAGACGACCA+TGG + chr4.1:87442668-87442687 None:intergenic 50.0%
GCAGTGACAGTTGTACCATC+TGG - chr4.1:87442878-87442897 MS.gene054572:CDS 50.0%
CGTGCAAAGGATCCAACTGG+TGG - chr4.1:87442950-87442969 MS.gene054572:CDS 55.0%
GAGACACGGGTCCATTTCCA+AGG + chr4.1:87442592-87442611 None:intergenic 55.0%
TCCACTGTGATGCCCGCTAA+AGG - chr4.1:87442164-87442183 MS.gene054572:CDS 55.0%
! ACCTTTAGCGGGCATCACAG+TGG + chr4.1:87442168-87442187 None:intergenic 55.0%
! AGCGGGCATCACAGTGGATT+TGG + chr4.1:87442162-87442181 None:intergenic 55.0%
! GCGGGCATCACAGTGGATTT+GGG + chr4.1:87442161-87442180 None:intergenic 55.0%
! TGAAGGCAAGAGCTCTGGTG+TGG - chr4.1:87442562-87442581 MS.gene054572:CDS 55.0%
!! GGTGTGGTTGGCCTTGGAAA+TGG - chr4.1:87442578-87442597 MS.gene054572:CDS 55.0%
!! TGGTGGGGTACCGACTGAAT+AGG + chr4.1:87442204-87442223 None:intergenic 55.0%
! GGCAAGAGCTCTGGTGTGGT+TGG - chr4.1:87442566-87442585 MS.gene054572:CDS 60.0%
!! AGCTCTGGTGTGGTTGGCCT+TGG - chr4.1:87442572-87442591 MS.gene054572:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 87442101 87443237 87442101 ID=MS.gene054572
chr4.1 mRNA 87442101 87443237 87442101 ID=MS.gene054572.t1;Parent=MS.gene054572
chr4.1 exon 87442101 87443237 87442101 ID=MS.gene054572.t1.exon1;Parent=MS.gene054572.t1
chr4.1 CDS 87442101 87443237 87442101 ID=cds.MS.gene054572.t1;Parent=MS.gene054572.t1
Gene Sequence

>MS.gene054572

ATGCGTCGTTCCATCGATCATGTCGACCATTACTACAAACATTCCCTTACTAACACACCCAAATCCACTGTGATGCCCGCTAAAGGTGAGTTTCTTATGACCTATTCAGTCGGTACCCCACCATTTAAGTTATATGGTATTGTTGATACAGGTAGTGACATGGTTTGGCTTCAATCTAAGCCTTGTGAACACTGTTACAACCAAACGACTCCTATTTTTAATCCTTTGAATTCATCAAGTTACAAAAACAATATTCCTTGTTTGTCTAAAAAATGTAAGTCTTTTGATTCTACCTCTTGTAATAAAAACAAAACTTGCGAATATAGTATTAAGTATGGTGATGGATCAATATCACAAGGAGATCTTAGTTTGGAAACTCTTACATTAGATTCCACCTCTGGTTCTTCTATTTCATTTCCTAAAACCGTGATAGGTTTTGGACATAACAATACATTGTCATTTGAAGGCAAGAGCTCTGGTGTGGTTGGCCTTGGAAATGGACCCGTGTCTCTAATAAATCAATTGGGTTCATCAATTGGTGGAAAGTTCTCTTATTGTTTGGGGCCATGGTCGTCTAACTCAACGAGCAAACTCAATTTTGGAGAAGCTGCTATAGTTTCTGGTCATGGAGTTGTGTCAACTCCTTTAGTGAAAAAAGACCCAAAAATTGCTTACTTAATAACATTGAAAGCATTTAGTGTTGGAAACAAAAGAGTAGAATTTTCAAAGCAATCAAATGGTAGTGTTGATGGTAACATCATAGTTGATTCAGGTACGGCAGTGACAGTTGTACCATCTGGTATTTATCATAATTTGGTATCAGCTGTGGTAAAATCGGTTAAACTGAAGCGTGCAAAGGATCCAACTGGTGGTTTTCTTAATCTTTGTTATTCCGTTGCATCAAATAAATATGATTTTCCCATAATCACTGCACATTTTGAAGGTGCAGATGTTAAGTTGCATTCTATTAGCACCTTTGCTCGAGTTTCTGATGGGGTTGTTTGTTTGACTCTTGTCCGATCTCTACTCAATAAAGGCATCTTTGGGAATTTTGCTCAACAGAATTTGTTGGTTGGTTATGATCTTCAACAAAAAATTGTGTCTTTTAAGCCTACTGATTGTACCAAACCATATTGA
Protein sequence

>MS.gene054572.t1

MRRSIDHVDHYYKHSLTNTPKSTVMPAKGEFLMTYSVGTPPFKLYGIVDTGSDMVWLQSKPCEHCYNQTTPIFNPLNSSSYKNNIPCLSKKCKSFDSTSCNKNKTCEYSIKYGDGSISQGDLSLETLTLDSTSGSSISFPKTVIGFGHNNTLSFEGKSSGVVGLGNGPVSLINQLGSSIGGKFSYCLGPWSSNSTSKLNFGEAAIVSGHGVVSTPLVKKDPKIAYLITLKAFSVGNKRVEFSKQSNGSVDGNIIVDSGTAVTVVPSGIYHNLVSAVVKSVKLKRAKDPTGGFLNLCYSVASNKYDFPIITAHFEGADVKLHSISTFARVSDGVVCLTLVRSLLNKGIFGNFAQQNLLVGYDLQQKIVSFKPTDCTKPY