Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054620.t1 | XP_003603003.1 | 93.3 | 299 | 17 | 2 | 1 | 296 | 1 | 299 | 7.20E-136 | 493.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054620.t1 | G7J5T8 | 93.3 | 299 | 17 | 2 | 1 | 296 | 1 | 299 | 5.2e-136 | 493.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054620 | MS.gene38739 | 0.911914 | 3.54E-83 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054620.t1 | MTR_3g101320 | 96.321 | 299 | 8 | 2 | 1 | 296 | 1 | 299 | 0.0 | 569 |
| MS.gene054620.t1 | MTR_1g021070 | 47.432 | 331 | 105 | 12 | 1 | 292 | 1 | 301 | 1.20e-56 | 185 |
| MS.gene054620.t1 | MTR_4g086620 | 37.764 | 331 | 146 | 11 | 1 | 296 | 1 | 306 | 3.01e-48 | 164 |
| MS.gene054620.t1 | MTR_1g033900 | 38.367 | 245 | 114 | 11 | 55 | 289 | 42 | 259 | 1.14e-29 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054620.t1 | AT2G26530 | 39.339 | 333 | 111 | 13 | 26 | 289 | 6 | 316 | 2.52e-35 | 130 |
| MS.gene054620.t1 | AT2G15760 | 34.754 | 305 | 146 | 10 | 16 | 289 | 14 | 296 | 2.33e-29 | 114 |
| MS.gene054620.t1 | AT2G26530 | 37.888 | 322 | 104 | 13 | 26 | 289 | 6 | 289 | 3.26e-29 | 113 |
| MS.gene054620.t1 | AT3G62630 | 26.647 | 334 | 154 | 12 | 25 | 289 | 47 | 358 | 9.23e-13 | 68.6 |
Find 51 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCTTATAAACAAGGGATTT+TGG | 0.080684 | 3.4:-89240114 | MS.gene054620:CDS |
| CCCAAAATCCCTTGTTTATA+AGG | 0.135090 | 3.4:+89240113 | None:intergenic |
| GAAGTTTCTTCATCGAATTC+TGG | 0.254643 | 3.4:-89240524 | MS.gene054620:CDS |
| ACACCGGAATCCTTTCTTCT+AGG | 0.275361 | 3.4:+89240206 | None:intergenic |
| AATTTCTTGTTGTATGAAAC+AGG | 0.288569 | 3.4:+89240284 | None:intergenic |
| CCTTCTTCACCAAAACGTTT+TGG | 0.298663 | 3.4:-89240863 | MS.gene054620:CDS |
| CTTCGAAGAGCTCTAGAATA+TGG | 0.331029 | 3.4:-89240382 | MS.gene054620:CDS |
| AAATCTTCACCAAAACGTTT+TGG | 0.332204 | 3.4:+89240854 | None:intergenic |
| GAAAAGTTCTTCAGCTGAAC+GGG | 0.337791 | 3.4:+89240730 | None:intergenic |
| TTCATACAACAAGAAATTAT+CGG | 0.348167 | 3.4:-89240279 | MS.gene054620:CDS |
| TTGGGTAGGTTAGCTGGGTT+AGG | 0.381728 | 3.4:-89240095 | MS.gene054620:CDS |
| GTGTATTGTGATGTTCTATA+TGG | 0.383379 | 3.4:+89240476 | None:intergenic |
| GTTGAAATCGAAATGATCTA+TGG | 0.384481 | 3.4:+89240910 | None:intergenic |
| AATTGAGAGTAAAACCTTGA+TGG | 0.395223 | 3.4:+89240809 | None:intergenic |
| AATAAGAAAGATTCATCTCT+TGG | 0.423616 | 3.4:-89240587 | MS.gene054620:CDS |
| AAAGATTCATCTCTTGGTAA+TGG | 0.445966 | 3.4:-89240581 | MS.gene054620:CDS |
| CAATTTGAAGATGAAGATGC+TGG | 0.451481 | 3.4:-89240791 | MS.gene054620:CDS |
| GGAGATTTAGCAGATTCTAA+AGG | 0.457397 | 3.4:+89240689 | None:intergenic |
| CTTCATCGAATTCTGGTCGT+AGG | 0.458023 | 3.4:-89240517 | MS.gene054620:CDS |
| AGAGCTCTTCGAAGAAGAAA+CGG | 0.494593 | 3.4:+89240391 | None:intergenic |
| GAATCCTTTCTTCTAGGTCT+CGG | 0.498133 | 3.4:+89240212 | None:intergenic |
| AACAAGAAATTATCGGAAGA+AGG | 0.500623 | 3.4:-89240272 | MS.gene054620:CDS |
| TTCATCGAATTCTGGTCGTA+GGG | 0.509111 | 3.4:-89240516 | MS.gene054620:CDS |
| GTAAAACCTTGATGGGCTAG+TGG | 0.520757 | 3.4:+89240817 | None:intergenic |
| ATTGAGAGTAAAACCTTGAT+GGG | 0.527633 | 3.4:+89240810 | None:intergenic |
| AGACCTAGAAGAAAGGATTC+CGG | 0.531090 | 3.4:-89240209 | MS.gene054620:CDS |
| TCTATGGTAGGACTCTCTCT+AGG | 0.534313 | 3.4:+89240926 | None:intergenic |
| GATGAAGAAACTTCAGAAGC+TGG | 0.548105 | 3.4:+89240533 | None:intergenic |
| GGAATCTCAGAAGATCTAAA+CGG | 0.549858 | 3.4:+89240233 | None:intergenic |
| GTGCACCCACTAGCCCATCA+AGG | 0.555600 | 3.4:-89240823 | MS.gene054620:CDS |
| AAATTATCGGAAGAAGGAAA+AGG | 0.566338 | 3.4:-89240266 | MS.gene054620:CDS |
| ACTCTATTTGGAGATAGAAG+AGG | 0.566647 | 3.4:+89240668 | None:intergenic |
| CTATGGTAGGACTCTCTCTA+GGG | 0.570266 | 3.4:+89240927 | None:intergenic |
| GGAAAAGTTCTTCAGCTGAA+CGG | 0.574814 | 3.4:+89240729 | None:intergenic |
| CGTAGTGCATCAGAAGGAAG+AGG | 0.575692 | 3.4:-89240335 | MS.gene054620:CDS |
| AAATCGAAATGATCTATGGT+AGG | 0.578754 | 3.4:+89240914 | None:intergenic |
| CACATGAATTGCATTATGCT+AGG | 0.583385 | 3.4:-89240178 | MS.gene054620:CDS |
| GAAGAAGGAGCACTTAGATA+AGG | 0.583615 | 3.4:+89240878 | None:intergenic |
| CAATTCATGTGCAGAAACAC+CGG | 0.592002 | 3.4:+89240190 | None:intergenic |
| GATTCCGAGACCTAGAAGAA+AGG | 0.594485 | 3.4:-89240216 | MS.gene054620:CDS |
| TTGTTTCGTAGTGCATCAGA+AGG | 0.599350 | 3.4:-89240341 | MS.gene054620:CDS |
| ACATTCTTGCCTTATAAACA+AGG | 0.601432 | 3.4:-89240122 | MS.gene054620:CDS |
| TAAAACCTTGATGGGCTAGT+GGG | 0.601581 | 3.4:+89240818 | None:intergenic |
| CATTCTTGCCTTATAAACAA+GGG | 0.601665 | 3.4:-89240121 | MS.gene054620:CDS |
| CTTGGTAATGGAGAAAGAAG+AGG | 0.603750 | 3.4:-89240569 | MS.gene054620:CDS |
| ATTTCTTGTTGTATGAAACA+GGG | 0.615031 | 3.4:+89240285 | None:intergenic |
| AACATCACAATACACATGGG+TGG | 0.638441 | 3.4:-89240468 | MS.gene054620:CDS |
| AAGGCTTGATTACACCACCA+TGG | 0.673762 | 3.4:+89240708 | None:intergenic |
| ATAGAACATCACAATACACA+TGG | 0.678148 | 3.4:-89240472 | MS.gene054620:CDS |
| AAAAGTTCTTCAGCTGAACG+GGG | 0.695702 | 3.4:+89240731 | None:intergenic |
| TAGAACATCACAATACACAT+GGG | 0.724735 | 3.4:-89240471 | MS.gene054620:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTCATACAACAAGAAATTAT+CGG | - | chr3.4:89240747-89240766 | MS.gene054620:CDS | 20.0% |
| !!! | GATGAATCTTTCTTATTTTT+AGG | + | chr3.4:89240436-89240455 | None:intergenic | 20.0% |
| ! | AAAAAATCTCCAATAGCAAA+AGG | - | chr3.4:89240382-89240401 | MS.gene054620:CDS | 25.0% |
| ! | AAAAGGTAAAAAAGCAATCA+TGG | - | chr3.4:89240399-89240418 | MS.gene054620:CDS | 25.0% |
| ! | AATAAGAAAGATTCATCTCT+TGG | - | chr3.4:89240439-89240458 | MS.gene054620:CDS | 25.0% |
| ! | ACATAAACAATCACAAAAAG+AGG | - | chr3.4:89240591-89240610 | MS.gene054620:CDS | 25.0% |
| !! | AATTTCTTGTTGTATGAAAC+AGG | + | chr3.4:89240745-89240764 | None:intergenic | 25.0% |
| !! | ATTTCTTGTTGTATGAAACA+GGG | + | chr3.4:89240744-89240763 | None:intergenic | 25.0% |
| AAAGATTCATCTCTTGGTAA+TGG | - | chr3.4:89240445-89240464 | MS.gene054620:CDS | 30.0% | |
| AAATCGAAATGATCTATGGT+AGG | + | chr3.4:89240115-89240134 | None:intergenic | 30.0% | |
| AAATTATCGGAAGAAGGAAA+AGG | - | chr3.4:89240760-89240779 | MS.gene054620:CDS | 30.0% | |
| AACAAGAAATTATCGGAAGA+AGG | - | chr3.4:89240754-89240773 | MS.gene054620:CDS | 30.0% | |
| AATTGAGAGTAAAACCTTGA+TGG | + | chr3.4:89240220-89240239 | None:intergenic | 30.0% | |
| ACATTCTTGCCTTATAAACA+AGG | - | chr3.4:89240904-89240923 | MS.gene054620:CDS | 30.0% | |
| ATAGAACATCACAATACACA+TGG | - | chr3.4:89240554-89240573 | MS.gene054620:CDS | 30.0% | |
| ATTGAGAGTAAAACCTTGAT+GGG | + | chr3.4:89240219-89240238 | None:intergenic | 30.0% | |
| CATTCTTGCCTTATAAACAA+GGG | - | chr3.4:89240905-89240924 | MS.gene054620:CDS | 30.0% | |
| GTGTATTGTGATGTTCTATA+TGG | + | chr3.4:89240553-89240572 | None:intergenic | 30.0% | |
| GTTGAAATCGAAATGATCTA+TGG | + | chr3.4:89240119-89240138 | None:intergenic | 30.0% | |
| TAGAACATCACAATACACAT+GGG | - | chr3.4:89240555-89240574 | MS.gene054620:CDS | 30.0% | |
| ! | AAATCTTCACCAAAACGTTT+TGG | + | chr3.4:89240175-89240194 | None:intergenic | 30.0% |
| ! | CCTTATAAACAAGGGATTTT+GGG | - | chr3.4:89240913-89240932 | MS.gene054620:CDS | 30.0% |
| !!! | GCTTTTTTACCTTTTGCTAT+TGG | + | chr3.4:89240394-89240413 | None:intergenic | 30.0% |
| !!! | GGAGATTTTTTGACTCTATT+TGG | + | chr3.4:89240373-89240392 | None:intergenic | 30.0% |
| ACTCTATTTGGAGATAGAAG+AGG | + | chr3.4:89240361-89240380 | None:intergenic | 35.0% | |
| CAATTTGAAGATGAAGATGC+TGG | - | chr3.4:89240235-89240254 | MS.gene054620:CDS | 35.0% | |
| CACATGAATTGCATTATGCT+AGG | - | chr3.4:89240848-89240867 | MS.gene054620:CDS | 35.0% | |
| GAAGTTTCTTCATCGAATTC+TGG | - | chr3.4:89240502-89240521 | MS.gene054620:CDS | 35.0% | |
| GGAATCTCAGAAGATCTAAA+CGG | + | chr3.4:89240796-89240815 | None:intergenic | 35.0% | |
| GGAGATTTAGCAGATTCTAA+AGG | + | chr3.4:89240340-89240359 | None:intergenic | 35.0% | |
| ! | CCCAAAATCCCTTGTTTATA+AGG | + | chr3.4:89240916-89240935 | None:intergenic | 35.0% |
| ! | GCCTTATAAACAAGGGATTT+TGG | - | chr3.4:89240912-89240931 | MS.gene054620:CDS | 35.0% |
| !! | ATAAACAAGGGATTTTGGGT+AGG | - | chr3.4:89240917-89240936 | MS.gene054620:CDS | 35.0% |
| !! | GAAACAGGGTATTTTCTGAA+TGG | + | chr3.4:89240730-89240749 | None:intergenic | 35.0% |
| AAAAGTTCTTCAGCTGAACG+GGG | + | chr3.4:89240298-89240317 | None:intergenic | 40.0% | |
| AACATCACAATACACATGGG+TGG | - | chr3.4:89240558-89240577 | MS.gene054620:CDS | 40.0% | |
| AGACCTAGAAGAAAGGATTC+CGG | - | chr3.4:89240817-89240836 | MS.gene054620:CDS | 40.0% | |
| AGAGCTCTTCGAAGAAGAAA+CGG | + | chr3.4:89240638-89240657 | None:intergenic | 40.0% | |
| CAATTCATGTGCAGAAACAC+CGG | + | chr3.4:89240839-89240858 | None:intergenic | 40.0% | |
| GAAAAGTTCTTCAGCTGAAC+GGG | + | chr3.4:89240299-89240318 | None:intergenic | 40.0% | |
| GAATCCTTTCTTCTAGGTCT+CGG | + | chr3.4:89240817-89240836 | None:intergenic | 40.0% | |
| GATGAAGAAACTTCAGAAGC+TGG | + | chr3.4:89240496-89240515 | None:intergenic | 40.0% | |
| GGAAAAGTTCTTCAGCTGAA+CGG | + | chr3.4:89240300-89240319 | None:intergenic | 40.0% | |
| TTCATCGAATTCTGGTCGTA+GGG | - | chr3.4:89240510-89240529 | MS.gene054620:CDS | 40.0% | |
| TTGTTTCGTAGTGCATCAGA+AGG | - | chr3.4:89240685-89240704 | MS.gene054620:CDS | 40.0% | |
| ! | CCTTCTTCACCAAAACGTTT+TGG | - | chr3.4:89240163-89240182 | MS.gene054620:CDS | 40.0% |
| ! | TCAGCTGAAGAACTTTTCCA+TGG | - | chr3.4:89240301-89240320 | MS.gene054620:CDS | 40.0% |
| !! | CCAAAACGTTTTGGTGAAGA+AGG | + | chr3.4:89240191-89240172 | None:intergenic | 40.0% |
| !! | CTTCGAAGAGCTCTAGAATA+TGG | - | chr3.4:89240644-89240663 | MS.gene054620:CDS | 40.0% |
| !! | CTTGGTAATGGAGAAAGAAG+AGG | - | chr3.4:89240457-89240476 | MS.gene054620:CDS | 40.0% |
| !! | GAAGAAGGAGCACTTAGATA+AGG | + | chr3.4:89240151-89240170 | None:intergenic | 40.0% |
| !! | TAAAACCTTGATGGGCTAGT+GGG | + | chr3.4:89240211-89240230 | None:intergenic | 40.0% |
| AAGGCTTGATTACACCACCA+TGG | + | chr3.4:89240321-89240340 | None:intergenic | 45.0% | |
| ACACCGGAATCCTTTCTTCT+AGG | + | chr3.4:89240823-89240842 | None:intergenic | 45.0% | |
| CTATGGTAGGACTCTCTCTA+GGG | + | chr3.4:89240102-89240121 | None:intergenic | 45.0% | |
| CTTCATCGAATTCTGGTCGT+AGG | - | chr3.4:89240509-89240528 | MS.gene054620:CDS | 45.0% | |
| GATTCCGAGACCTAGAAGAA+AGG | - | chr3.4:89240810-89240829 | MS.gene054620:CDS | 45.0% | |
| TCTATGGTAGGACTCTCTCT+AGG | + | chr3.4:89240103-89240122 | None:intergenic | 45.0% | |
| ! | GCTGAAGAACTTTTCCATGG+TGG | - | chr3.4:89240304-89240323 | MS.gene054620:CDS | 45.0% |
| !! | GGATTTTGGGTAGGTTAGCT+GGG | - | chr3.4:89240926-89240945 | MS.gene054620:CDS | 45.0% |
| !! | GTAAAACCTTGATGGGCTAG+TGG | + | chr3.4:89240212-89240231 | None:intergenic | 45.0% |
| CGTAGTGCATCAGAAGGAAG+AGG | - | chr3.4:89240691-89240710 | MS.gene054620:CDS | 50.0% | |
| ! | TTGGGTAGGTTAGCTGGGTT+AGG | - | chr3.4:89240931-89240950 | MS.gene054620:CDS | 50.0% |
| !! | GGGATTTTGGGTAGGTTAGC+TGG | - | chr3.4:89240925-89240944 | MS.gene054620:CDS | 50.0% |
| GTGCACCCACTAGCCCATCA+AGG | - | chr3.4:89240203-89240222 | MS.gene054620:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 89240079 | 89240969 | 89240079 | ID=MS.gene054620 |
| chr3.4 | mRNA | 89240079 | 89240969 | 89240079 | ID=MS.gene054620.t1;Parent=MS.gene054620 |
| chr3.4 | exon | 89240079 | 89240969 | 89240079 | ID=MS.gene054620.t1.exon1;Parent=MS.gene054620.t1 |
| chr3.4 | CDS | 89240079 | 89240969 | 89240079 | ID=cds.MS.gene054620.t1;Parent=MS.gene054620.t1 |
| Gene Sequence |
| Protein sequence |
