Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38739.t1 | XP_003603003.1 | 93 | 299 | 19 | 1 | 1 | 297 | 1 | 299 | 4.20E-136 | 494.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38739.t1 | G7J5T8 | 93.0 | 299 | 19 | 1 | 1 | 297 | 1 | 299 | 3.1e-136 | 494.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053879 | MS.gene38739 | 0.831436 | 1.67E-55 | -1.69E-46 |
| MS.gene054620 | MS.gene38739 | 0.911914 | 3.54E-83 | -1.69E-46 |
| MS.gene055150 | MS.gene38739 | 0.847559 | 1.07E-59 | -1.69E-46 |
| MS.gene057622 | MS.gene38739 | 0.824457 | 7.99E-54 | -1.69E-46 |
| MS.gene057999 | MS.gene38739 | 0.820732 | 5.87E-53 | -1.69E-46 |
| MS.gene058592 | MS.gene38739 | 0.809622 | 1.73E-50 | -1.69E-46 |
| MS.gene05952 | MS.gene38739 | 0.818027 | 2.43E-52 | -1.69E-46 |
| MS.gene060977 | MS.gene38739 | 0.805563 | 1.26E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38739.t1 | MTR_3g101320 | 95.987 | 299 | 10 | 1 | 1 | 297 | 1 | 299 | 0.0 | 571 |
| MS.gene38739.t1 | MTR_1g021070 | 47.432 | 331 | 106 | 12 | 1 | 293 | 1 | 301 | 3.20e-57 | 186 |
| MS.gene38739.t1 | MTR_4g086620 | 37.838 | 333 | 144 | 13 | 1 | 297 | 1 | 306 | 2.76e-47 | 161 |
| MS.gene38739.t1 | MTR_1g033900 | 38.174 | 241 | 121 | 9 | 55 | 290 | 42 | 259 | 5.49e-29 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38739.t1 | AT2G26530 | 39.640 | 333 | 111 | 13 | 26 | 290 | 6 | 316 | 2.49e-36 | 132 |
| MS.gene38739.t1 | AT2G26530 | 38.199 | 322 | 104 | 13 | 26 | 290 | 6 | 289 | 3.80e-30 | 115 |
| MS.gene38739.t1 | AT2G15760 | 34.641 | 306 | 146 | 11 | 16 | 290 | 14 | 296 | 1.82e-29 | 114 |
| MS.gene38739.t1 | AT3G62630 | 26.048 | 334 | 157 | 12 | 25 | 290 | 47 | 358 | 1.86e-12 | 67.4 |
Find 49 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCTTATAAACAAGGGATTT+TGG | 0.080684 | 3.3:-83960688 | MS.gene38739:CDS |
| CCCAAAATCCCTTGTTTATA+AGG | 0.135090 | 3.3:+83960687 | None:intergenic |
| GAAGTTTCTTCATCGAATTC+TGG | 0.254643 | 3.3:-83961101 | MS.gene38739:CDS |
| ACACCGGAATCCTTTCTTCT+AGG | 0.275361 | 3.3:+83960780 | None:intergenic |
| AATTTCTTGTTGTATGAAAC+AGG | 0.288569 | 3.3:+83960858 | None:intergenic |
| CCTTCTTCACCAAAACGTTT+TGG | 0.298663 | 3.3:-83961440 | MS.gene38739:CDS |
| CTTCGAAGAGCTCTAGAATA+TGG | 0.331029 | 3.3:-83960956 | MS.gene38739:CDS |
| AAATCTTCACCAAAACGTTT+TGG | 0.332204 | 3.3:+83961431 | None:intergenic |
| TTCATACAACAAGAAATTAT+CGG | 0.348167 | 3.3:-83960853 | MS.gene38739:CDS |
| TTGGGTAGGTTAGCTGGGTT+AGG | 0.381728 | 3.3:-83960669 | MS.gene38739:CDS |
| GTGTATTGTGATGTTCTATA+TGG | 0.383379 | 3.3:+83961053 | None:intergenic |
| GTTGAAATCGAAATGATCTA+TGG | 0.384481 | 3.3:+83961487 | None:intergenic |
| AATTGAGAGTAAAACCTTGA+TGG | 0.395223 | 3.3:+83961386 | None:intergenic |
| AATAAGAAAGATTCATCTCT+TGG | 0.423616 | 3.3:-83961164 | MS.gene38739:CDS |
| AAAGATTCATCTCTTGGTAA+TGG | 0.445966 | 3.3:-83961158 | MS.gene38739:CDS |
| CAATTTGAAGATGAAGATGC+TGG | 0.451481 | 3.3:-83961368 | MS.gene38739:CDS |
| GGAGATTTAGCAGATTCTAA+AGG | 0.457397 | 3.3:+83961266 | None:intergenic |
| CTTCATCGAATTCTGGTCGT+AGG | 0.458023 | 3.3:-83961094 | MS.gene38739:CDS |
| AGAGCTCTTCGAAGAAGAAA+CGG | 0.494593 | 3.3:+83960965 | None:intergenic |
| GAATCCTTTCTTCTAGGTCT+CGG | 0.498133 | 3.3:+83960786 | None:intergenic |
| AACAAGAAATTATCGGAAGA+AGG | 0.500623 | 3.3:-83960846 | MS.gene38739:CDS |
| TTCATCGAATTCTGGTCGTA+GGG | 0.509111 | 3.3:-83961093 | MS.gene38739:CDS |
| GTAAAACCTTGATGGGCTAG+TGG | 0.520757 | 3.3:+83961394 | None:intergenic |
| ATTGAGAGTAAAACCTTGAT+GGG | 0.527633 | 3.3:+83961387 | None:intergenic |
| AGACCTAGAAGAAAGGATTC+CGG | 0.531090 | 3.3:-83960783 | MS.gene38739:CDS |
| TCTATGGTAGGACTCTCTCT+AGG | 0.534313 | 3.3:+83961503 | None:intergenic |
| GATGAAGAAACTTCAGAAGC+TGG | 0.548105 | 3.3:+83961110 | None:intergenic |
| GGAATCTCAGAAGATCTAAA+CGG | 0.549858 | 3.3:+83960807 | None:intergenic |
| GTGCACCCACTAGCCCATCA+AGG | 0.555600 | 3.3:-83961400 | MS.gene38739:CDS |
| AAATTATCGGAAGAAGGAAA+AGG | 0.566338 | 3.3:-83960840 | MS.gene38739:CDS |
| ACTCTATTTGGAGATAGAAG+AGG | 0.566647 | 3.3:+83961245 | None:intergenic |
| CTATGGTAGGACTCTCTCTA+GGG | 0.570266 | 3.3:+83961504 | None:intergenic |
| CGTAGTGCATCAGAAGGAAG+AGG | 0.575692 | 3.3:-83960909 | MS.gene38739:CDS |
| AAATCGAAATGATCTATGGT+AGG | 0.578754 | 3.3:+83961491 | None:intergenic |
| CACATGAATTGCATTATGCT+AGG | 0.583385 | 3.3:-83960752 | MS.gene38739:CDS |
| GAAGAAGGAGCACTTAGATA+AGG | 0.583615 | 3.3:+83961455 | None:intergenic |
| CAATTCATGTGCAGAAACAC+CGG | 0.592002 | 3.3:+83960764 | None:intergenic |
| GATTCCGAGACCTAGAAGAA+AGG | 0.594485 | 3.3:-83960790 | MS.gene38739:CDS |
| TTGTTTCGTAGTGCATCAGA+AGG | 0.599350 | 3.3:-83960915 | MS.gene38739:CDS |
| ACATTCTTGCCTTATAAACA+AGG | 0.601432 | 3.3:-83960696 | MS.gene38739:CDS |
| TAAAACCTTGATGGGCTAGT+GGG | 0.601581 | 3.3:+83961395 | None:intergenic |
| CATTCTTGCCTTATAAACAA+GGG | 0.601665 | 3.3:-83960695 | MS.gene38739:CDS |
| CTTGGTAATGGAGAAAGAAG+AGG | 0.603750 | 3.3:-83961146 | MS.gene38739:CDS |
| ATTTCTTGTTGTATGAAACA+GGG | 0.615031 | 3.3:+83960859 | None:intergenic |
| GGAAAAGTTCTTCAGCTGAA+CGG | 0.624984 | 3.3:+83961306 | None:intergenic |
| AACATCACAATACACATGGG+TGG | 0.638441 | 3.3:-83961045 | MS.gene38739:CDS |
| AAGGCTTGATTACACCACCA+TGG | 0.673762 | 3.3:+83961285 | None:intergenic |
| ATAGAACATCACAATACACA+TGG | 0.678148 | 3.3:-83961049 | MS.gene38739:CDS |
| TAGAACATCACAATACACAT+GGG | 0.724735 | 3.3:-83961048 | MS.gene38739:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTCATACAACAAGAAATTAT+CGG | - | chr3.3:83961324-83961343 | MS.gene38739:CDS | 20.0% |
| !!! | GATGAATCTTTCTTATTTTT+AGG | + | chr3.3:83961010-83961029 | None:intergenic | 20.0% |
| ! | AAAAAATCTCCAATAGCAAA+AGG | - | chr3.3:83960956-83960975 | MS.gene38739:CDS | 25.0% |
| ! | AAAAGGTAAAAAAGCAATCA+TGG | - | chr3.3:83960973-83960992 | MS.gene38739:CDS | 25.0% |
| ! | AATAAGAAAGATTCATCTCT+TGG | - | chr3.3:83961013-83961032 | MS.gene38739:CDS | 25.0% |
| ! | ACATAAACAATCACAAAAAG+AGG | - | chr3.3:83961168-83961187 | MS.gene38739:CDS | 25.0% |
| !! | AATTTCTTGTTGTATGAAAC+AGG | + | chr3.3:83961322-83961341 | None:intergenic | 25.0% |
| !! | ATTTCTTGTTGTATGAAACA+GGG | + | chr3.3:83961321-83961340 | None:intergenic | 25.0% |
| AAAGATTCATCTCTTGGTAA+TGG | - | chr3.3:83961019-83961038 | MS.gene38739:CDS | 30.0% | |
| AAATCGAAATGATCTATGGT+AGG | + | chr3.3:83960689-83960708 | None:intergenic | 30.0% | |
| AAATTATCGGAAGAAGGAAA+AGG | - | chr3.3:83961337-83961356 | MS.gene38739:CDS | 30.0% | |
| AACAAGAAATTATCGGAAGA+AGG | - | chr3.3:83961331-83961350 | MS.gene38739:CDS | 30.0% | |
| AATTGAGAGTAAAACCTTGA+TGG | + | chr3.3:83960794-83960813 | None:intergenic | 30.0% | |
| ACATTCTTGCCTTATAAACA+AGG | - | chr3.3:83961481-83961500 | MS.gene38739:CDS | 30.0% | |
| ATAGAACATCACAATACACA+TGG | - | chr3.3:83961128-83961147 | MS.gene38739:CDS | 30.0% | |
| ATTGAGAGTAAAACCTTGAT+GGG | + | chr3.3:83960793-83960812 | None:intergenic | 30.0% | |
| CATTCTTGCCTTATAAACAA+GGG | - | chr3.3:83961482-83961501 | MS.gene38739:CDS | 30.0% | |
| GTGTATTGTGATGTTCTATA+TGG | + | chr3.3:83961127-83961146 | None:intergenic | 30.0% | |
| GTTGAAATCGAAATGATCTA+TGG | + | chr3.3:83960693-83960712 | None:intergenic | 30.0% | |
| TAGAACATCACAATACACAT+GGG | - | chr3.3:83961129-83961148 | MS.gene38739:CDS | 30.0% | |
| ! | AAATCTTCACCAAAACGTTT+TGG | + | chr3.3:83960749-83960768 | None:intergenic | 30.0% |
| ! | CCTTATAAACAAGGGATTTT+GGG | - | chr3.3:83961490-83961509 | MS.gene38739:CDS | 30.0% |
| !!! | GCTTTTTTACCTTTTGCTAT+TGG | + | chr3.3:83960968-83960987 | None:intergenic | 30.0% |
| !!! | GGAGATTTTTTGACTCTATT+TGG | + | chr3.3:83960947-83960966 | None:intergenic | 30.0% |
| ACTCTATTTGGAGATAGAAG+AGG | + | chr3.3:83960935-83960954 | None:intergenic | 35.0% | |
| CAATTTGAAGATGAAGATGC+TGG | - | chr3.3:83960809-83960828 | MS.gene38739:CDS | 35.0% | |
| CACATGAATTGCATTATGCT+AGG | - | chr3.3:83961425-83961444 | MS.gene38739:CDS | 35.0% | |
| GAAGTTTCTTCATCGAATTC+TGG | - | chr3.3:83961076-83961095 | MS.gene38739:CDS | 35.0% | |
| GGAATCTCAGAAGATCTAAA+CGG | + | chr3.3:83961373-83961392 | None:intergenic | 35.0% | |
| GGAGATTTAGCAGATTCTAA+AGG | + | chr3.3:83960914-83960933 | None:intergenic | 35.0% | |
| ! | CCCAAAATCCCTTGTTTATA+AGG | + | chr3.3:83961493-83961512 | None:intergenic | 35.0% |
| ! | GCCTTATAAACAAGGGATTT+TGG | - | chr3.3:83961489-83961508 | MS.gene38739:CDS | 35.0% |
| !! | ATAAACAAGGGATTTTGGGT+AGG | - | chr3.3:83961494-83961513 | MS.gene38739:CDS | 35.0% |
| !! | GAAACAGGGTATTTTCTGAA+TGG | + | chr3.3:83961307-83961326 | None:intergenic | 35.0% |
| AACATCACAATACACATGGG+TGG | - | chr3.3:83961132-83961151 | MS.gene38739:CDS | 40.0% | |
| AGACCTAGAAGAAAGGATTC+CGG | - | chr3.3:83961394-83961413 | MS.gene38739:CDS | 40.0% | |
| AGAGCTCTTCGAAGAAGAAA+CGG | + | chr3.3:83961215-83961234 | None:intergenic | 40.0% | |
| CAATTCATGTGCAGAAACAC+CGG | + | chr3.3:83961416-83961435 | None:intergenic | 40.0% | |
| GAATCCTTTCTTCTAGGTCT+CGG | + | chr3.3:83961394-83961413 | None:intergenic | 40.0% | |
| GATGAAGAAACTTCAGAAGC+TGG | + | chr3.3:83961070-83961089 | None:intergenic | 40.0% | |
| GGAAAAGTTCTTCAGCTGAA+CGG | + | chr3.3:83960874-83960893 | None:intergenic | 40.0% | |
| TTCATCGAATTCTGGTCGTA+GGG | - | chr3.3:83961084-83961103 | MS.gene38739:CDS | 40.0% | |
| TTGTTTCGTAGTGCATCAGA+AGG | - | chr3.3:83961262-83961281 | MS.gene38739:CDS | 40.0% | |
| ! | CCTTCTTCACCAAAACGTTT+TGG | - | chr3.3:83960737-83960756 | MS.gene38739:CDS | 40.0% |
| ! | TCAGCTGAAGAACTTTTCCA+TGG | - | chr3.3:83960875-83960894 | MS.gene38739:CDS | 40.0% |
| !! | CCAAAACGTTTTGGTGAAGA+AGG | + | chr3.3:83960765-83960746 | None:intergenic | 40.0% |
| !! | CTTCGAAGAGCTCTAGAATA+TGG | - | chr3.3:83961221-83961240 | MS.gene38739:CDS | 40.0% |
| !! | CTTGGTAATGGAGAAAGAAG+AGG | - | chr3.3:83961031-83961050 | MS.gene38739:CDS | 40.0% |
| !! | GAAGAAGGAGCACTTAGATA+AGG | + | chr3.3:83960725-83960744 | None:intergenic | 40.0% |
| !! | TAAAACCTTGATGGGCTAGT+GGG | + | chr3.3:83960785-83960804 | None:intergenic | 40.0% |
| AAGGCTTGATTACACCACCA+TGG | + | chr3.3:83960895-83960914 | None:intergenic | 45.0% | |
| ACACCGGAATCCTTTCTTCT+AGG | + | chr3.3:83961400-83961419 | None:intergenic | 45.0% | |
| CTATGGTAGGACTCTCTCTA+GGG | + | chr3.3:83960676-83960695 | None:intergenic | 45.0% | |
| CTTCATCGAATTCTGGTCGT+AGG | - | chr3.3:83961083-83961102 | MS.gene38739:CDS | 45.0% | |
| GATTCCGAGACCTAGAAGAA+AGG | - | chr3.3:83961387-83961406 | MS.gene38739:CDS | 45.0% | |
| TCTATGGTAGGACTCTCTCT+AGG | + | chr3.3:83960677-83960696 | None:intergenic | 45.0% | |
| ! | GCTGAAGAACTTTTCCATGG+TGG | - | chr3.3:83960878-83960897 | MS.gene38739:CDS | 45.0% |
| !! | GGATTTTGGGTAGGTTAGCT+GGG | - | chr3.3:83961503-83961522 | MS.gene38739:CDS | 45.0% |
| !! | GTAAAACCTTGATGGGCTAG+TGG | + | chr3.3:83960786-83960805 | None:intergenic | 45.0% |
| CGTAGTGCATCAGAAGGAAG+AGG | - | chr3.3:83961268-83961287 | MS.gene38739:CDS | 50.0% | |
| ! | TTGGGTAGGTTAGCTGGGTT+AGG | - | chr3.3:83961508-83961527 | MS.gene38739:CDS | 50.0% |
| !! | GGGATTTTGGGTAGGTTAGC+TGG | - | chr3.3:83961502-83961521 | MS.gene38739:CDS | 50.0% |
| GTGCACCCACTAGCCCATCA+AGG | - | chr3.3:83960777-83960796 | MS.gene38739:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 83960653 | 83961546 | 83960653 | ID=MS.gene38739 |
| chr3.3 | mRNA | 83960653 | 83961546 | 83960653 | ID=MS.gene38739.t1;Parent=MS.gene38739 |
| chr3.3 | exon | 83960653 | 83961546 | 83960653 | ID=MS.gene38739.t1.exon1;Parent=MS.gene38739.t1 |
| chr3.3 | CDS | 83960653 | 83961546 | 83960653 | ID=cds.MS.gene38739.t1;Parent=MS.gene38739.t1 |
| Gene Sequence |
| Protein sequence |
