Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054770.t1 | XP_003614248.1 | 97.5 | 236 | 6 | 0 | 1 | 236 | 1 | 236 | 1.90E-126 | 461.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054770.t1 | G7JY47 | 97.5 | 236 | 6 | 0 | 1 | 236 | 1 | 236 | 1.3e-126 | 461.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054770 | MS.gene26659 | 0.803725 | 3.04E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054770.t1 | MTR_5g047050 | 97.458 | 236 | 6 | 0 | 1 | 236 | 1 | 236 | 1.11e-175 | 482 |
| MS.gene054770.t1 | MTR_4g109880 | 46.725 | 229 | 111 | 4 | 9 | 236 | 4 | 222 | 3.05e-64 | 199 |
| MS.gene054770.t1 | MTR_3g072390 | 35.366 | 164 | 93 | 4 | 74 | 236 | 73 | 224 | 2.03e-23 | 94.4 |
| MS.gene054770.t1 | MTR_2g055760 | 32.143 | 140 | 84 | 3 | 79 | 216 | 16 | 146 | 7.42e-14 | 68.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054770.t1 | AT1G69080 | 44.309 | 246 | 104 | 5 | 1 | 236 | 1 | 223 | 2.00e-64 | 200 |
| MS.gene054770.t1 | AT2G03720 | 53.757 | 173 | 74 | 5 | 66 | 236 | 64 | 232 | 1.39e-57 | 182 |
| MS.gene054770.t1 | AT2G03720 | 53.846 | 169 | 72 | 5 | 70 | 236 | 1 | 165 | 2.94e-56 | 177 |
| MS.gene054770.t1 | AT1G69080 | 41.463 | 246 | 97 | 6 | 1 | 236 | 1 | 209 | 3.63e-55 | 176 |
| MS.gene054770.t1 | AT5G17390 | 47.977 | 173 | 81 | 4 | 68 | 236 | 118 | 285 | 8.68e-50 | 164 |
| MS.gene054770.t1 | AT3G03290 | 45.087 | 173 | 86 | 4 | 68 | 236 | 107 | 274 | 4.74e-46 | 154 |
| MS.gene054770.t1 | AT1G44760 | 37.427 | 171 | 88 | 4 | 66 | 236 | 62 | 213 | 6.73e-30 | 111 |
| MS.gene054770.t1 | AT1G44760 | 37.427 | 171 | 88 | 4 | 66 | 236 | 133 | 284 | 3.79e-29 | 110 |
| MS.gene054770.t1 | AT4G13450 | 27.404 | 208 | 112 | 6 | 66 | 236 | 14 | 219 | 8.40e-17 | 76.6 |
| MS.gene054770.t1 | AT1G48960 | 30.488 | 164 | 99 | 6 | 67 | 230 | 5 | 153 | 5.04e-14 | 69.3 |
Find 40 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGCCATACAATGTGCATTT+TGG | 0.179754 | 5.3:-34274952 | None:intergenic |
| AGAAGAAAGAGTAAGAAAAT+TGG | 0.268808 | 5.3:+34274983 | MS.gene054770:CDS |
| AAGACGAGGCGTGGCACTTT+TGG | 0.282461 | 5.3:+34274838 | MS.gene054770:CDS |
| TACTTACACTGTTCAGAATC+AGG | 0.343889 | 5.3:+34274060 | MS.gene054770:CDS |
| TGCCCATTGCAAAGCACCTT+TGG | 0.367782 | 5.3:-34274035 | None:intergenic |
| TCATGCTTGGCTTGTATAGT+TGG | 0.372638 | 5.3:-34273872 | None:intergenic |
| TATCCAAAATGCACATTGTA+TGG | 0.401167 | 5.3:+34274949 | MS.gene054770:CDS |
| AAGAGAGGAGCAAGGTTACC+AGG | 0.459111 | 5.3:+34273806 | MS.gene054770:CDS |
| AGCCAAAGGTGCTTTGCAAT+GGG | 0.470225 | 5.3:+34274033 | MS.gene054770:CDS |
| AAGCCAAAGGTGCTTTGCAA+TGG | 0.482336 | 5.3:+34274032 | MS.gene054770:CDS |
| AGAAAGAGTAAGAAAATTGG+AGG | 0.492506 | 5.3:+34274986 | MS.gene054770:CDS |
| TGATGATGAAGCTACTAGTA+AGG | 0.513401 | 5.3:+34274234 | MS.gene054770:CDS |
| AGTAACAGAAGGGAAAGAAA+AGG | 0.525585 | 5.3:+34274790 | MS.gene054770:CDS |
| AACAAGGGTCAGTAGTTAGT+AGG | 0.536437 | 5.3:+34273978 | MS.gene054770:CDS |
| GTAACAGAAGGGAAAGAAAA+GGG | 0.538384 | 5.3:+34274791 | MS.gene054770:CDS |
| TGGGCTGGCAATAGAGTCAC+TGG | 0.549351 | 5.3:+34274908 | MS.gene054770:CDS |
| GGCGAACGCACCCTCGCATG+TGG | 0.550940 | 5.3:-34273851 | None:intergenic |
| AAACCTTGTTTAGAATTCGA+AGG | 0.560200 | 5.3:-34274112 | None:intergenic |
| TCGAATTAGGCCACATGCGA+GGG | 0.562880 | 5.3:+34273841 | MS.gene054770:CDS |
| TTTGACGATGGTGTGAAACA+AGG | 0.566437 | 5.3:+34273962 | MS.gene054770:CDS |
| GAAGCAAGTTCATAAGCCCT+TGG | 0.570979 | 5.3:-34274265 | None:intergenic |
| TTCCAAAACATGGGCAAGAG+AGG | 0.576269 | 5.3:+34273791 | None:intergenic |
| CGTCTTCTAATGATGTGGGC+TGG | 0.577564 | 5.3:+34274893 | MS.gene054770:CDS |
| CATTAGAAGACGCCATGTTG+TGG | 0.577627 | 5.3:-34274882 | None:intergenic |
| ATTGAGATTGCAGTAACAGA+AGG | 0.585136 | 5.3:+34274779 | MS.gene054770:CDS |
| CATGGCGTCTTCTAATGATG+TGG | 0.588320 | 5.3:+34274888 | MS.gene054770:CDS |
| GAAAGAGTAAGAAAATTGGA+GGG | 0.591657 | 5.3:+34274987 | MS.gene054770:CDS |
| ATCGAATTAGGCCACATGCG+AGG | 0.593025 | 5.3:+34273840 | MS.gene054770:CDS |
| CAATAGAGTCACTGGTGGAG+TGG | 0.594867 | 5.3:+34274916 | MS.gene054770:CDS |
| TTGACGATGGTGTGAAACAA+GGG | 0.607583 | 5.3:+34273963 | MS.gene054770:CDS |
| TTGAGATTGCAGTAACAGAA+GGG | 0.610661 | 5.3:+34274780 | MS.gene054770:CDS |
| GAATTCATGCAGTGTTTGCG+AGG | 0.613944 | 5.3:+34273934 | MS.gene054770:CDS |
| AAGCCTTCGAATTCTAAACA+AGG | 0.615497 | 5.3:+34274109 | MS.gene054770:CDS |
| ATGGCGTCTTCTAATGATGT+GGG | 0.632260 | 5.3:+34274889 | MS.gene054770:CDS |
| CTAGTAAGGAGACAGATCCA+AGG | 0.637212 | 5.3:+34274248 | MS.gene054770:CDS |
| TAGTAAGGAGACAGATCCAA+GGG | 0.640856 | 5.3:+34274249 | MS.gene054770:CDS |
| GCTGGCAATAGAGTCACTGG+TGG | 0.677998 | 5.3:+34274911 | MS.gene054770:CDS |
| TGCGAGGAGAAATTTGACGA+TGG | 0.693381 | 5.3:+34273950 | MS.gene054770:CDS |
| ACATGGGCAAGAGAGGAGCA+AGG | 0.696922 | 5.3:+34273798 | None:intergenic |
| TTGTAATCTGAAAATGCCTG+AGG | 0.734520 | 5.3:+34274303 | MS.gene054770:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTACTTAAAATTATTTCAA+AGG | - | chr5.3:34274535-34274554 | None:intergenic | 10.0% |
| !! | CATTATAATGTAAATAAACA+AGG | + | chr5.3:34274644-34274663 | MS.gene054770:intron | 15.0% |
| !!! | TATAAATCTAAATTTTCAGA+TGG | - | chr5.3:34274355-34274374 | None:intergenic | 15.0% |
| !! | AAGGTTAAATGTTAAAATCT+CGG | - | chr5.3:34274516-34274535 | None:intergenic | 20.0% |
| !! | ATTATCATGCAAAGAATTTA+AGG | - | chr5.3:34274617-34274636 | None:intergenic | 20.0% |
| ! | AAAACATACAATGATATCGT+TGG | - | chr5.3:34274144-34274163 | None:intergenic | 25.0% |
| ! | AAATGTTGAGACTGTATATA+TGG | + | chr5.3:34274741-34274760 | MS.gene054770:intron | 25.0% |
| ! | AGAAGAAAGAGTAAGAAAAT+TGG | + | chr5.3:34274983-34275002 | MS.gene054770:CDS | 25.0% |
| ! | CAAGAATGATTCATTCAATA+TGG | + | chr5.3:34274204-34274223 | MS.gene054770:intron | 25.0% |
| ! | TGGATGGAATTTATTACATT+TGG | - | chr5.3:34274674-34274693 | None:intergenic | 25.0% |
| !! | TATATTGTCACTTACTTACA+TGG | - | chr5.3:34274694-34274713 | None:intergenic | 25.0% |
| !! | TTTAACACTCTGAAAAATAG+AGG | + | chr5.3:34274402-34274421 | MS.gene054770:intron | 25.0% |
| AATCTCAATTTGTACCTATC+AGG | - | chr5.3:34274768-34274787 | None:intergenic | 30.0% | |
| AGAAAGAGTAAGAAAATTGG+AGG | + | chr5.3:34274986-34275005 | MS.gene054770:CDS | 30.0% | |
| GAAAGAGTAAGAAAATTGGA+GGG | + | chr5.3:34274987-34275006 | MS.gene054770:CDS | 30.0% | |
| TATCCAAAATGCACATTGTA+TGG | + | chr5.3:34274949-34274968 | MS.gene054770:CDS | 30.0% | |
| TTCATCTTGTACCATTGTTT+AGG | + | chr5.3:34274451-34274470 | MS.gene054770:intron | 30.0% | |
| ! | AAACCTTGTTTAGAATTCGA+AGG | - | chr5.3:34274115-34274134 | None:intergenic | 30.0% |
| ! | CTAAGCGTCATAAAGATTTT+TGG | + | chr5.3:34275023-34275042 | MS.gene054770:CDS | 30.0% |
| ! | GTTTTTGCTTGAATCGAATT+AGG | + | chr5.3:34273828-34273847 | MS.gene054770:CDS | 30.0% |
| !!! | ATCATCACTTTTTTCATGCT+TGG | - | chr5.3:34273888-34273907 | None:intergenic | 30.0% |
| !!! | TCATAAAGATTTTTGGCTCT+TGG | + | chr5.3:34275030-34275049 | MS.gene054770:CDS | 30.0% |
| AACAATGGTACAAGATGAAG+TGG | - | chr5.3:34274450-34274469 | None:intergenic | 35.0% | |
| AAGCCTTCGAATTCTAAACA+AGG | + | chr5.3:34274109-34274128 | MS.gene054770:CDS | 35.0% | |
| ACTGTATATATGGTCCTGAT+AGG | + | chr5.3:34274751-34274770 | MS.gene054770:intron | 35.0% | |
| AGAAAAGGGACCAAAAATAG+TGG | + | chr5.3:34274805-34274824 | MS.gene054770:CDS | 35.0% | |
| AGTAACAGAAGGGAAAGAAA+AGG | + | chr5.3:34274790-34274809 | MS.gene054770:CDS | 35.0% | |
| ATTCGATTCAAGCAAAAACC+TGG | - | chr5.3:34273827-34273846 | None:intergenic | 35.0% | |
| ATTGAGATTGCAGTAACAGA+AGG | + | chr5.3:34274779-34274798 | MS.gene054770:CDS | 35.0% | |
| GTAACAGAAGGGAAAGAAAA+GGG | + | chr5.3:34274791-34274810 | MS.gene054770:CDS | 35.0% | |
| TACTTACACTGTTCAGAATC+AGG | + | chr5.3:34274060-34274079 | MS.gene054770:CDS | 35.0% | |
| TTGAGATTGCAGTAACAGAA+GGG | + | chr5.3:34274780-34274799 | MS.gene054770:CDS | 35.0% | |
| TTGTAATCTGAAAATGCCTG+AGG | + | chr5.3:34274303-34274322 | MS.gene054770:CDS | 35.0% | |
| ! | TCAGATGGTAGCATAGTTTT+AGG | - | chr5.3:34274340-34274359 | None:intergenic | 35.0% |
| ! | TGATGATGAAGCTACTAGTA+AGG | + | chr5.3:34274234-34274253 | MS.gene054770:CDS | 35.0% |
| ! | TTGCCATACAATGTGCATTT+TGG | - | chr5.3:34274955-34274974 | None:intergenic | 35.0% |
| ! | TTGTCACTTACTTACATGGA+TGG | - | chr5.3:34274690-34274709 | None:intergenic | 35.0% |
| ! | TTTGCTTCCTCCACTATTTT+TGG | - | chr5.3:34274818-34274837 | None:intergenic | 35.0% |
| !!! | GATTCTAGTTTTGAAGCCAA+AGG | + | chr5.3:34274019-34274038 | MS.gene054770:CDS | 35.0% |
| AAAGGGACCAAAAATAGTGG+AGG | + | chr5.3:34274808-34274827 | MS.gene054770:CDS | 40.0% | |
| AACAAGGGTCAGTAGTTAGT+AGG | + | chr5.3:34273978-34273997 | MS.gene054770:CDS | 40.0% | |
| AGAAAAAGAGGTCCACAACA+TGG | + | chr5.3:34274870-34274889 | MS.gene054770:CDS | 40.0% | |
| ATGGCGTCTTCTAATGATGT+GGG | + | chr5.3:34274889-34274908 | MS.gene054770:CDS | 40.0% | |
| TAGTAAGGAGACAGATCCAA+GGG | + | chr5.3:34274249-34274268 | MS.gene054770:CDS | 40.0% | |
| TCGCGATACAACCTAAACAA+TGG | - | chr5.3:34274465-34274484 | None:intergenic | 40.0% | |
| TTGACGATGGTGTGAAACAA+GGG | + | chr5.3:34273963-34273982 | MS.gene054770:CDS | 40.0% | |
| TTTGACGATGGTGTGAAACA+AGG | + | chr5.3:34273962-34273981 | MS.gene054770:CDS | 40.0% | |
| !! | TCATGCTTGGCTTGTATAGT+TGG | - | chr5.3:34273875-34273894 | None:intergenic | 40.0% |
| CATGGCGTCTTCTAATGATG+TGG | + | chr5.3:34274888-34274907 | MS.gene054770:CDS | 45.0% | |
| CATTAGAAGACGCCATGTTG+TGG | - | chr5.3:34274885-34274904 | None:intergenic | 45.0% | |
| CTAGTAAGGAGACAGATCCA+AGG | + | chr5.3:34274248-34274267 | MS.gene054770:CDS | 45.0% | |
| GAAGCAAGTTCATAAGCCCT+TGG | - | chr5.3:34274268-34274287 | None:intergenic | 45.0% | |
| GAATTCATGCAGTGTTTGCG+AGG | + | chr5.3:34273934-34273953 | MS.gene054770:CDS | 45.0% | |
| TGCGAGGAGAAATTTGACGA+TGG | + | chr5.3:34273950-34273969 | MS.gene054770:CDS | 45.0% | |
| ! | TTAGGAGCATAACTCACCTC+AGG | - | chr5.3:34274322-34274341 | None:intergenic | 45.0% |
| !! | AAGCCAAAGGTGCTTTGCAA+TGG | + | chr5.3:34274032-34274051 | MS.gene054770:CDS | 45.0% |
| !! | AGCCAAAGGTGCTTTGCAAT+GGG | + | chr5.3:34274033-34274052 | MS.gene054770:CDS | 45.0% |
| !!! | TGGTTTTGGGGCAGAAAAAG+AGG | + | chr5.3:34274858-34274877 | MS.gene054770:CDS | 45.0% |
| AAGAGAGGAGCAAGGTTACC+AGG | + | chr5.3:34273806-34273825 | MS.gene054770:CDS | 50.0% | |
| ATCGAATTAGGCCACATGCG+AGG | + | chr5.3:34273840-34273859 | MS.gene054770:CDS | 50.0% | |
| CAATAGAGTCACTGGTGGAG+TGG | + | chr5.3:34274916-34274935 | MS.gene054770:CDS | 50.0% | |
| CGTCTTCTAATGATGTGGGC+TGG | + | chr5.3:34274893-34274912 | MS.gene054770:CDS | 50.0% | |
| GTGGAGGAAGCAAAAAGACG+AGG | + | chr5.3:34274824-34274843 | MS.gene054770:CDS | 50.0% | |
| TCGAATTAGGCCACATGCGA+GGG | + | chr5.3:34273841-34273860 | MS.gene054770:CDS | 50.0% | |
| ! | TGCCCATTGCAAAGCACCTT+TGG | - | chr5.3:34274038-34274057 | None:intergenic | 50.0% |
| !!! | AGGCGTGGCACTTTTGGTTT+TGG | + | chr5.3:34274844-34274863 | MS.gene054770:CDS | 50.0% |
| !!! | GCGTGGCACTTTTGGTTTTG+GGG | + | chr5.3:34274846-34274865 | MS.gene054770:CDS | 50.0% |
| !!! | GGCGTGGCACTTTTGGTTTT+GGG | + | chr5.3:34274845-34274864 | MS.gene054770:CDS | 50.0% |
| GCTGGCAATAGAGTCACTGG+TGG | + | chr5.3:34274911-34274930 | MS.gene054770:CDS | 55.0% | |
| GGAAGCAAAAAGACGAGGCG+TGG | + | chr5.3:34274829-34274848 | MS.gene054770:CDS | 55.0% | |
| ! | AAGACGAGGCGTGGCACTTT+TGG | + | chr5.3:34274838-34274857 | MS.gene054770:CDS | 55.0% |
| !! | TGGGCTGGCAATAGAGTCAC+TGG | + | chr5.3:34274908-34274927 | MS.gene054770:CDS | 55.0% |
| GGCGAACGCACCCTCGCATG+TGG | - | chr5.3:34273854-34273873 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 34273800 | 34275057 | 34273800 | ID=MS.gene054770 |
| chr5.3 | mRNA | 34273800 | 34275057 | 34273800 | ID=MS.gene054770.t1;Parent=MS.gene054770 |
| chr5.3 | exon | 34273800 | 34274130 | 34273800 | ID=MS.gene054770.t1.exon1;Parent=MS.gene054770.t1 |
| chr5.3 | CDS | 34273800 | 34274130 | 34273800 | ID=cds.MS.gene054770.t1;Parent=MS.gene054770.t1 |
| chr5.3 | exon | 34274230 | 34274324 | 34274230 | ID=MS.gene054770.t1.exon2;Parent=MS.gene054770.t1 |
| chr5.3 | CDS | 34274230 | 34274324 | 34274230 | ID=cds.MS.gene054770.t1;Parent=MS.gene054770.t1 |
| chr5.3 | exon | 34274773 | 34275057 | 34274773 | ID=MS.gene054770.t1.exon3;Parent=MS.gene054770.t1 |
| chr5.3 | CDS | 34274773 | 34275057 | 34274773 | ID=cds.MS.gene054770.t1;Parent=MS.gene054770.t1 |
| Gene Sequence |
| Protein sequence |
