AlfalfaGEDB | MS.gene26659

M. sativa cultivar XinJiangDaYe / MS.gene26659

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26659.t1	XP_013456959.1	100	84	0	0	69	152	275	358	4.80E-27	131

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26659.t1	I3T3J6	100.0	84	0	0	69	152	275	358	3.5e-27	131.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene26659	0.854613	1.10E-61	-1.69E-46
MS.gene049190	MS.gene26659	0.833858	4.19E-56	-1.69E-46
MS.gene049227	MS.gene26659	0.835857	1.31E-56	-1.69E-46
MS.gene049254	MS.gene26659	0.818327	2.08E-52	-1.69E-46
MS.gene049350	MS.gene26659	0.829028	6.47E-55	-1.69E-46
MS.gene049404	MS.gene26659	0.810438	1.15E-50	-1.69E-46
MS.gene049418	MS.gene26659	0.84481	6.00E-59	-1.69E-46
MS.gene049501	MS.gene26659	0.80989	1.51E-50	-1.69E-46
MS.gene049672	MS.gene26659	0.807197	5.69E-50	-1.69E-46
MS.gene049711	MS.gene26659	0.891323	4.41E-74	-1.69E-46
MS.gene049807	MS.gene26659	0.853785	1.90E-61	-1.69E-46
MS.gene049824	MS.gene26659	0.821166	4.67E-53	-1.69E-46
MS.gene049825	MS.gene26659	0.803088	4.12E-49	-1.69E-46
MS.gene049850	MS.gene26659	0.839903	1.20E-57	-1.69E-46
MS.gene049873	MS.gene26659	0.803297	3.73E-49	-1.69E-46
MS.gene049887	MS.gene26659	0.805678	1.19E-49	-1.69E-46
MS.gene050221	MS.gene26659	0.800339	1.51E-48	-1.69E-46
MS.gene050241	MS.gene26659	0.812884	3.39E-51	-1.69E-46
MS.gene050273	MS.gene26659	0.804737	1.87E-49	-1.69E-46
MS.gene050559	MS.gene26659	0.823966	1.04E-53	-1.69E-46
MS.gene050561	MS.gene26659	0.822754	2.00E-53	-1.69E-46
MS.gene050562	MS.gene26659	0.817529	3.15E-52	-1.69E-46
MS.gene050657	MS.gene26659	0.857144	1.99E-62	-1.69E-46
MS.gene050807	MS.gene26659	0.810636	1.04E-50	-1.69E-46
MS.gene050901	MS.gene26659	0.804381	2.22E-49	-1.69E-46
MS.gene051018	MS.gene26659	0.851788	7.06E-61	-1.69E-46
MS.gene051021	MS.gene26659	0.878524	2.62E-69	-1.69E-46
MS.gene051198	MS.gene26659	0.814231	1.71E-51	-1.69E-46
MS.gene051247	MS.gene26659	0.840416	8.81E-58	-1.69E-46
MS.gene051279	MS.gene26659	0.874954	4.51E-68	-1.69E-46
MS.gene051388	MS.gene26659	0.841928	3.53E-58	-1.69E-46
MS.gene05150	MS.gene26659	0.802942	4.42E-49	-1.69E-46
MS.gene05157	MS.gene26659	0.817932	2.55E-52	-1.69E-46
MS.gene051664	MS.gene26659	0.828195	1.03E-54	-1.69E-46
MS.gene051700	MS.gene26659	0.826961	2.04E-54	-1.69E-46
MS.gene051719	MS.gene26659	0.878737	2.20E-69	-1.69E-46
MS.gene051720	MS.gene26659	0.897636	1.16E-76	-1.69E-46
MS.gene051744	MS.gene26659	0.868457	6.44E-66	-1.69E-46
MS.gene051957	MS.gene26659	0.820446	6.83E-53	-1.69E-46
MS.gene052000	MS.gene26659	0.802744	4.85E-49	-1.69E-46
MS.gene052037	MS.gene26659	0.811994	5.30E-51	-1.69E-46
MS.gene052133	MS.gene26659	0.818968	1.49E-52	-1.69E-46
MS.gene052234	MS.gene26659	0.825829	3.79E-54	-1.69E-46
MS.gene052459	MS.gene26659	0.807643	4.57E-50	-1.69E-46
MS.gene052463	MS.gene26659	0.836586	8.58E-57	-1.69E-46
MS.gene052516	MS.gene26659	0.861554	9.45E-64	-1.69E-46
MS.gene052519	MS.gene26659	0.807692	4.47E-50	-1.69E-46
MS.gene052582	MS.gene26659	0.809383	1.94E-50	-1.69E-46
MS.gene052584	MS.gene26659	0.821223	4.53E-53	-1.69E-46
MS.gene052609	MS.gene26659	0.806589	7.64E-50	-1.69E-46
MS.gene052611	MS.gene26659	0.804811	1.81E-49	-1.69E-46
MS.gene052665	MS.gene26659	0.807554	4.78E-50	-1.69E-46
MS.gene052731	MS.gene26659	0.801464	8.89E-49	-1.69E-46
MS.gene052732	MS.gene26659	0.865362	6.24E-65	-1.69E-46
MS.gene052773	MS.gene26659	0.804523	2.07E-49	-1.69E-46
MS.gene053079	MS.gene26659	0.831338	1.77E-55	-1.69E-46
MS.gene053122	MS.gene26659	0.829776	4.26E-55	-1.69E-46
MS.gene053144	MS.gene26659	0.927245	1.54E-91	-1.69E-46
MS.gene053164	MS.gene26659	0.82218	2.72E-53	-1.69E-46
MS.gene053171	MS.gene26659	0.84492	5.61E-59	-1.69E-46
MS.gene053174	MS.gene26659	0.822537	2.25E-53	-1.69E-46
MS.gene053226	MS.gene26659	0.849707	2.72E-60	-1.69E-46
MS.gene053536	MS.gene26659	0.855463	6.20E-62	-1.69E-46
MS.gene053538	MS.gene26659	0.851734	7.32E-61	-1.69E-46
MS.gene053540	MS.gene26659	0.806103	9.68E-50	-1.69E-46
MS.gene053581	MS.gene26659	0.84755	1.08E-59	-1.69E-46
MS.gene053592	MS.gene26659	0.804473	2.12E-49	-1.69E-46
MS.gene053599	MS.gene26659	0.837858	4.06E-57	-1.69E-46
MS.gene053621	MS.gene26659	0.803052	4.19E-49	-1.69E-46
MS.gene053998	MS.gene26659	0.811103	8.28E-51	-1.69E-46
MS.gene05422	MS.gene26659	0.807447	5.03E-50	-1.69E-46
MS.gene05423	MS.gene26659	0.81811	2.33E-52	-1.69E-46
MS.gene054480	MS.gene26659	0.804441	2.16E-49	-1.69E-46
MS.gene054550	MS.gene26659	0.811856	5.68E-51	-1.69E-46
MS.gene054554	MS.gene26659	0.805722	1.16E-49	-1.69E-46
MS.gene054584	MS.gene26659	0.926935	2.36E-91	-1.69E-46
MS.gene05458	MS.gene26659	0.816241	6.12E-52	-1.69E-46
MS.gene054651	MS.gene26659	0.858333	8.86E-63	-1.69E-46
MS.gene054770	MS.gene26659	0.803725	3.04E-49	-1.69E-46
MS.gene054775	MS.gene26659	0.817648	2.96E-52	-1.69E-46
MS.gene054801	MS.gene26659	0.803207	3.89E-49	-1.69E-46
MS.gene054824	MS.gene26659	0.803874	2.83E-49	-1.69E-46
MS.gene054991	MS.gene26659	0.812517	4.07E-51	-1.69E-46
MS.gene055083	MS.gene26659	0.834111	3.62E-56	-1.69E-46
MS.gene055103	MS.gene26659	0.800356	1.50E-48	-1.69E-46
MS.gene055144	MS.gene26659	0.834091	3.66E-56	-1.69E-46
MS.gene055465	MS.gene26659	0.878782	2.13E-69	-1.69E-46
MS.gene055466	MS.gene26659	0.873067	1.96E-67	-1.69E-46
MS.gene055467	MS.gene26659	0.816676	4.89E-52	-1.69E-46
MS.gene055468	MS.gene26659	0.862071	6.57E-64	-1.69E-46
MS.gene055469	MS.gene26659	0.849323	3.48E-60	-1.69E-46
MS.gene055512	MS.gene26659	0.805076	1.59E-49	-1.69E-46
MS.gene055513	MS.gene26659	0.812436	4.24E-51	-1.69E-46
MS.gene055537	MS.gene26659	0.906494	1.40E-80	-1.69E-46
MS.gene055580	MS.gene26659	-0.891913	2.57E-74	-1.69E-46
MS.gene05574	MS.gene26659	0.805106	1.57E-49	-1.69E-46
MS.gene055764	MS.gene26659	0.802758	4.82E-49	-1.69E-46
MS.gene055778	MS.gene26659	0.804708	1.90E-49	-1.69E-46
MS.gene055890	MS.gene26659	0.840507	8.34E-58	-1.69E-46
MS.gene055891	MS.gene26659	0.83582	1.34E-56	-1.69E-46
MS.gene056043	MS.gene26659	-0.866952	1.96E-65	-1.69E-46
MS.gene056050	MS.gene26659	0.848202	7.12E-60	-1.69E-46
MS.gene056169	MS.gene26659	0.826084	3.29E-54	-1.69E-46
MS.gene056174	MS.gene26659	0.827089	1.90E-54	-1.69E-46
MS.gene056228	MS.gene26659	0.822034	2.94E-53	-1.69E-46
MS.gene056308	MS.gene26659	0.835464	1.65E-56	-1.69E-46
MS.gene056366	MS.gene26659	0.801885	7.29E-49	-1.69E-46
MS.gene056396	MS.gene26659	0.804054	2.60E-49	-1.69E-46
MS.gene056507	MS.gene26659	0.836585	8.59E-57	-1.69E-46
MS.gene056553	MS.gene26659	0.806645	7.44E-50	-1.69E-46
MS.gene056595	MS.gene26659	0.805141	1.54E-49	-1.69E-46
MS.gene05686	MS.gene26659	0.845525	3.85E-59	-1.69E-46
MS.gene057082	MS.gene26659	0.84152	4.52E-58	-1.69E-46
MS.gene057102	MS.gene26659	0.806302	8.79E-50	-1.69E-46
MS.gene057132	MS.gene26659	0.810469	1.13E-50	-1.69E-46
MS.gene057166	MS.gene26659	0.807446	5.04E-50	-1.69E-46
MS.gene057294	MS.gene26659	0.844175	8.90E-59	-1.69E-46
MS.gene057314	MS.gene26659	0.806485	8.04E-50	-1.69E-46
MS.gene057444	MS.gene26659	0.81341	2.60E-51	-1.69E-46
MS.gene057915	MS.gene26659	0.819315	1.24E-52	-1.69E-46
MS.gene058010	MS.gene26659	0.823145	1.62E-53	-1.69E-46
MS.gene058037	MS.gene26659	0.807096	5.97E-50	-1.69E-46
MS.gene058087	MS.gene26659	0.819046	1.43E-52	-1.69E-46
MS.gene058330	MS.gene26659	0.80058	1.35E-48	-1.69E-46
MS.gene058339	MS.gene26659	0.845871	3.10E-59	-1.69E-46
MS.gene058492	MS.gene26659	0.812024	5.22E-51	-1.69E-46
MS.gene058625	MS.gene26659	0.85091	1.25E-60	-1.69E-46
MS.gene058649	MS.gene26659	0.825368	4.87E-54	-1.69E-46
MS.gene058668	MS.gene26659	0.861862	7.61E-64	-1.69E-46
MS.gene058852	MS.gene26659	0.80723	5.60E-50	-1.69E-46
MS.gene058945	MS.gene26659	0.814313	1.64E-51	-1.69E-46
MS.gene05894	MS.gene26659	0.855982	4.37E-62	-1.69E-46
MS.gene059015	MS.gene26659	0.867058	1.81E-65	-1.69E-46
MS.gene059084	MS.gene26659	0.819751	9.85E-53	-1.69E-46
MS.gene059154	MS.gene26659	0.825092	5.66E-54	-1.69E-46
MS.gene059170	MS.gene26659	0.820874	5.45E-53	-1.69E-46
MS.gene059272	MS.gene26659	0.829086	6.27E-55	-1.69E-46
MS.gene059424	MS.gene26659	0.846314	2.35E-59	-1.69E-46
MS.gene059425	MS.gene26659	0.803862	2.85E-49	-1.69E-46
MS.gene059426	MS.gene26659	0.81222	4.73E-51	-1.69E-46
MS.gene059570	MS.gene26659	0.82718	1.80E-54	-1.69E-46
MS.gene059601	MS.gene26659	0.8025	5.45E-49	-1.69E-46
MS.gene059602	MS.gene26659	0.803749	3.01E-49	-1.69E-46
MS.gene059625	MS.gene26659	0.846335	2.32E-59	-1.69E-46
MS.gene059965	MS.gene26659	0.800319	1.52E-48	-1.69E-46
MS.gene060312	MS.gene26659	0.807506	4.89E-50	-1.69E-46
MS.gene060429	MS.gene26659	0.809075	2.26E-50	-1.69E-46
MS.gene060519	MS.gene26659	0.863365	2.62E-64	-1.69E-46
MS.gene060587	MS.gene26659	0.841848	3.70E-58	-1.69E-46
MS.gene060603	MS.gene26659	0.828158	1.05E-54	-1.69E-46
MS.gene060884	MS.gene26659	0.820309	7.34E-53	-1.69E-46
MS.gene060886	MS.gene26659	0.81599	6.96E-52	-1.69E-46
MS.gene060962	MS.gene26659	0.802454	5.57E-49	-1.69E-46
MS.gene061100	MS.gene26659	0.819564	1.09E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26659.t1	MTR_4g088385	96.104	154	2	3	1	152	207	358	5.53e-91	268
MS.gene26659.t1	MTR_2g014400	52.381	147	56	5	13	151	120	260	1.76e-18	79.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 16 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTCAAACTGCTCTTGATTC+TGG	0.250818	4.1:-21861999	None:intergenic
TGTTGTTGTTGTTATTGTTC+TGG	0.280406	4.1:-21861597	None:intergenic
ATTGAGTTGTAACCTTCTTC+TGG	0.340437	4.1:-21861664	None:intergenic
TTGAGTTGTAACCTTCTTCT+GGG	0.440468	4.1:-21861663	None:intergenic
TTCAATGGAGATTCATCTAA+AGG	0.457617	4.1:+21861880	MS.gene26659:CDS
AATGAGTGACACAAGAGTTA+TGG	0.465302	4.1:+21861804	MS.gene26659:CDS
AAATACAATCCAACATTCAA+TGG	0.477159	4.1:+21861865	MS.gene26659:CDS
GAGTTGTGAACTCTGAGAAT+TGG	0.491359	4.1:+21861902	MS.gene26659:CDS
GATGAATCTCCATTGAATGT+TGG	0.492045	4.1:-21861874	None:intergenic
AACACAAAGTTCCCAGAAGA+AGG	0.504928	4.1:+21861652	MS.gene26659:CDS
AATAACAAGAACTCTATGAA+TGG	0.533344	4.1:+21861973	MS.gene26659:CDS
TACAACAATGGTGAGAGGCA+AGG	0.586180	4.1:+21861781	MS.gene26659:CDS
AGTAACAACAATTACAACAA+TGG	0.621284	4.1:+21861769	MS.gene26659:CDS
GACACAAGAGTTATGGAAGG+TGG	0.628338	4.1:+21861811	MS.gene26659:CDS
ACAATTACAACAATGGTGAG+AGG	0.652438	4.1:+21861776	MS.gene26659:CDS
AGTGACACAAGAGTTATGGA+AGG	0.692016	4.1:+21861808	MS.gene26659:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAATACAATCCAACATTCAA+TGG	+	chr4.1:21861865-21861884	MS.gene26659:CDS	25.0%
!	AATAACAAGAACTCTATGAA+TGG	+	chr4.1:21861973-21861992	MS.gene26659:CDS	25.0%
!	AGTAACAACAATTACAACAA+TGG	+	chr4.1:21861769-21861788	MS.gene26659:CDS	25.0%
!	TACAACAAAAAAGGTTACTT+TGG	+	chr4.1:21861925-21861944	MS.gene26659:CDS	25.0%
!!	TTTTTATGATGTTAACTCTG+AGG	+	chr4.1:21861840-21861859	MS.gene26659:CDS	25.0%
	GAGAATTGGTACAACAAAAA+AGG	+	chr4.1:21861916-21861935	MS.gene26659:CDS	30.0%
	TTCAATGGAGATTCATCTAA+AGG	+	chr4.1:21861880-21861899	MS.gene26659:CDS	30.0%
!	TGTTGTTGTTGTTATTGTTC+TGG	-	chr4.1:21861600-21861619	None:intergenic	30.0%
	AATGAGTGACACAAGAGTTA+TGG	+	chr4.1:21861804-21861823	MS.gene26659:CDS	35.0%
	ACAATTACAACAATGGTGAG+AGG	+	chr4.1:21861776-21861795	MS.gene26659:CDS	35.0%
	GATGAATCTCCATTGAATGT+TGG	-	chr4.1:21861877-21861896	None:intergenic	35.0%
!	ATTGAGTTGTAACCTTCTTC+TGG	-	chr4.1:21861667-21861686	None:intergenic	35.0%
!	TTGAGTTGTAACCTTCTTCT+GGG	-	chr4.1:21861666-21861685	None:intergenic	35.0%
	AACACAAAGTTCCCAGAAGA+AGG	+	chr4.1:21861652-21861671	MS.gene26659:CDS	40.0%
	AGTGACACAAGAGTTATGGA+AGG	+	chr4.1:21861808-21861827	MS.gene26659:CDS	40.0%
	CTTCAAACTGCTCTTGATTC+TGG	-	chr4.1:21862002-21862021	None:intergenic	40.0%
!	CGTAGTTGTTGTTGTTGTTC+TGG	-	chr4.1:21861696-21861715	None:intergenic	40.0%
!	GAGTTGTGAACTCTGAGAAT+TGG	+	chr4.1:21861902-21861921	MS.gene26659:CDS	40.0%
	GACACAAGAGTTATGGAAGG+TGG	+	chr4.1:21861811-21861830	MS.gene26659:CDS	45.0%
	TACAACAATGGTGAGAGGCA+AGG	+	chr4.1:21861781-21861800	MS.gene26659:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	21861589	21862047	21861589	ID=MS.gene26659
chr4.1	mRNA	21861589	21862047	21861589	ID=MS.gene26659.t1;Parent=MS.gene26659
chr4.1	exon	21861589	21862047	21861589	ID=MS.gene26659.t1.exon1;Parent=MS.gene26659.t1
chr4.1	CDS	21861589	21862047	21861589	ID=cds.MS.gene26659.t1;Parent=MS.gene26659.t1

Gene Sequence

>MS.gene26659

ATGGAAAACCAGAACAATAACAACAACAACAAGTATTTCTACAACAACAAAGATGCTTCTAGCAACACAAAGTTCCCAGAAGAAGGTTACAACTCAATGCAAAACCAGAACAACAACAACAACTACGAGAAGTATAACTACAACAACAAAGTTGCTGTTAATGACAAATACAGCTTCAAAAGTAACAACAATTACAACAATGGTGAGAGGCAAGGAATGAGTGACACAAGAGTTATGGAAGGTGGAAAATATTTTTATGATGTTAACTCTGAGGAGAAATACAATCCAACATTCAATGGAGATTCATCTAAAGGAGTTGTGAACTCTGAGAATTGGTACAACAAAAAAGGTTACTTTGGTAACAACAATGTGAACAATTATGAGAATAACAAGAACTCTATGAATGGTTACCAGAATCAAGAGCAGTTTGAAGATGATCAAGAAGAATTTGAGCCTTGA

Protein sequence

>MS.gene26659.t1

MENQNNNNNNKYFYNNKDASSNTKFPEEGYNSMQNQNNNNNYEKYNYNNKVAVNDKYSFKSNNNYNNGERQGMSDTRVMEGGKYFYDVNSEEKYNPTFNGDSSKGVVNSENWYNKKGYFGNNNVNNYENNKNSMNGYQNQEQFEDDQEEFEP