Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054844.t1 | XP_003607052.1 | 75.5 | 94 | 23 | 0 | 1 | 94 | 1 | 94 | 1.20E-31 | 145.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054844.t1 | G7JJU6 | 75.5 | 94 | 23 | 0 | 1 | 94 | 1 | 94 | 8.4e-32 | 145.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054844 | MS.gene054845 | 0.837308 | 5.61E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054844.t1 | MTR_6g008860 | 75.532 | 94 | 23 | 0 | 1 | 94 | 1 | 94 | 3.22e-48 | 150 |
| MS.gene054844.t1 | MTR_4g071650 | 75.532 | 94 | 23 | 0 | 1 | 94 | 1 | 94 | 3.22e-48 | 150 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTCTTTCATTTGTTTATATC+TGG | - | 50877:7221-7240 | MS.gene054844:intergenic | 25.0% |
| ! | TTTCATTTGTTTATATCTGG+TGG | - | 50877:7218-7237 | MS.gene054844:intergenic | 25.0% |
| !!! | TTTGATTTGTCCATAATCAA+TGG | + | 50877:7454-7473 | MS.gene054844:CDS | 25.0% |
| ATCAATGCATTGCTAATAGA+TGG | + | 50877:7298-7317 | MS.gene054844:CDS | 30.0% | |
| CATCAAATGCTTGAATCAAT+AGG | + | 50877:7346-7365 | MS.gene054844:CDS | 30.0% | |
| TAGCACCAGTCAATAAAATA+TGG | - | 50877:7411-7430 | MS.gene054844:intergenic | 30.0% | |
| ! | GCAATCCATATTTTATTGAC+TGG | + | 50877:7403-7422 | MS.gene054844:CDS | 30.0% |
| ! | AGCAATGCATTGATGAGATA+AGG | - | 50877:7292-7311 | MS.gene054844:intergenic | 35.0% |
| ! | ATTCGCATTTTCTTCTCATC+AGG | - | 50877:7244-7263 | MS.gene054844:intergenic | 35.0% |
| GCTTCTTGAGAACTATTTGC+AGG | - | 50877:7385-7404 | MS.gene054844:intergenic | 40.0% | |
| TGAGACTGCAACGAGACGCT+TGG | + | 50877:7321-7340 | MS.gene054844:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50877 | gene | 7202 | 7483 | 7202 | ID=MS.gene054844 |
| 50877 | mRNA | 7202 | 7483 | 7202 | ID=MS.gene054844.t1;Parent=MS.gene054844 |
| 50877 | exon | 7202 | 7483 | 7202 | ID=MS.gene054844.t1.exon1;Parent=MS.gene054844.t1 |
| 50877 | CDS | 7202 | 7483 | 7202 | ID=cds.MS.gene054844.t1;Parent=MS.gene054844.t1 |
| Gene Sequence |
| Protein sequence |
