Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054845.t1 | XP_003607052.1 | 78 | 109 | 24 | 0 | 1 | 109 | 1 | 109 | 3.90E-39 | 170.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054845.t1 | F4JZT3 | 30.3 | 76 | 53 | 0 | 33 | 108 | 18 | 93 | 1.2e-07 | 57.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054845.t1 | G7JJU6 | 78.0 | 109 | 24 | 0 | 1 | 109 | 1 | 109 | 2.8e-39 | 170.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene054845.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054844 | MS.gene054845 | 0.837308 | 5.61E-57 | -1.69E-46 |
| MS.gene054845 | MS.gene054846 | 0.960903 | 4.37E-119 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054845.t1 | MTR_6g008860 | 77.982 | 109 | 24 | 0 | 1 | 109 | 1 | 109 | 7.53e-59 | 178 |
| MS.gene054845.t1 | MTR_4g071650 | 77.982 | 109 | 24 | 0 | 1 | 109 | 1 | 109 | 7.53e-59 | 178 |
| MS.gene054845.t1 | MTR_7g099500 | 35.065 | 77 | 50 | 0 | 33 | 109 | 11 | 87 | 6.66e-13 | 60.8 |
| MS.gene054845.t1 | MTR_8g063580 | 35.065 | 77 | 50 | 0 | 33 | 109 | 12 | 88 | 2.15e-12 | 59.7 |
| MS.gene054845.t1 | MTR_7g098910 | 29.412 | 85 | 60 | 0 | 25 | 109 | 14 | 98 | 9.42e-11 | 55.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054845.t1 | AT5G26594 | 30.263 | 76 | 53 | 0 | 33 | 108 | 18 | 93 | 3.60e-11 | 56.6 |
Find 0 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGTTAGTAGTATTAATTAA+GGG | - | 50876:7159-7178 | MS.gene054845:intergenic | 15.0% |
| !! | AAAAGTTAACGTCATATTTA+CGG | + | 50876:6447-6466 | MS.gene054845:intron | 20.0% |
| !! | AATAATTTAATAATCCCCAT+GGG | + | 50876:6813-6832 | MS.gene054845:intron | 20.0% |
| !! | ATAAAGAAATTGTTTATACC+TGG | - | 50876:6351-6370 | MS.gene054845:intergenic | 20.0% |
| !! | CAAGTTAGTAGTATTAATTA+AGG | - | 50876:7160-7179 | MS.gene054845:intergenic | 20.0% |
| !! | GGACAAAAATAAAATCAAAT+GGG | - | 50876:7078-7097 | MS.gene054845:intergenic | 20.0% |
| !! | TAAAGAAATTGTTTATACCT+GGG | - | 50876:6350-6369 | MS.gene054845:intergenic | 20.0% |
| !! | TAATAATTTAATAATCCCCA+TGG | + | 50876:6812-6831 | MS.gene054845:intron | 20.0% |
| !! | TTATGAACATTACATGAAAT+GGG | - | 50876:7349-7368 | MS.gene054845:intergenic | 20.0% |
| !! | TTTAATACACATACAAACAA+AGG | + | 50876:7202-7221 | MS.gene054845:intron | 20.0% |
| !!! | TATTAATTAAGGGTATGTTT+AGG | - | 50876:7149-7168 | MS.gene054845:intergenic | 20.0% |
| ! | AAAGAAATTGTTTATACCTG+GGG | - | 50876:6349-6368 | MS.gene054845:intergenic | 25.0% |
| ! | AAGAAATGTGAAAAATGCAA+AGG | + | 50876:7284-7303 | MS.gene054845:intron | 25.0% |
| ! | AATGCATCTAGTAATTTGAA+AGG | - | 50876:7114-7133 | MS.gene054845:intergenic | 25.0% |
| ! | ACTACCACTAAAATAAATTG+TGG | + | 50876:7398-7417 | MS.gene054845:intron | 25.0% |
| ! | AGAAATGTGAAAAATGCAAA+GGG | + | 50876:7285-7304 | MS.gene054845:intron | 25.0% |
| ! | ATAATCTGATTGTCTAGATT+CGG | - | 50876:6705-6724 | MS.gene054845:intergenic | 25.0% |
| ! | ATGCATCTAGTAATTTGAAA+GGG | - | 50876:7113-7132 | MS.gene054845:intergenic | 25.0% |
| ! | CAAAAATAAAATCAAATGGG+TGG | - | 50876:7075-7094 | MS.gene054845:intergenic | 25.0% |
| ! | CTCTTTCATTTGTTTATATC+TGG | - | 50876:6089-6108 | MS.gene054845:intergenic | 25.0% |
| ! | GGGACAAAAATAAAATCAAA+TGG | - | 50876:7079-7098 | MS.gene054845:intergenic | 25.0% |
| ! | GTCTTTGGTTAAAATCATTT+AGG | + | 50876:6884-6903 | MS.gene054845:intron | 25.0% |
| ! | GTTATGAACATTACATGAAA+TGG | - | 50876:7350-7369 | MS.gene054845:intergenic | 25.0% |
| ! | TTTCATTTGTTTATATCTGG+TGG | - | 50876:6086-6105 | MS.gene054845:intergenic | 25.0% |
| !!! | ACAACCACAATTTATTTTAG+TGG | - | 50876:7405-7424 | MS.gene054845:intergenic | 25.0% |
| !!! | TAGTTGATAAGCATTAAGTT+TGG | - | 50876:6603-6622 | MS.gene054845:intergenic | 25.0% |
| !!! | TTTGATTTGTCCATAATCAA+TGG | + | 50876:6322-6341 | MS.gene054845:CDS | 25.0% |
| AAATGTGAAAAATGCAAAGG+GGG | + | 50876:7287-7306 | MS.gene054845:intron | 30.0% | |
| ATCAATGCATTGCTAATAGA+TGG | + | 50876:6166-6185 | MS.gene054845:CDS | 30.0% | |
| CATCAAATGCTTGAATCAAT+AGG | + | 50876:6214-6233 | MS.gene054845:CDS | 30.0% | |
| CATTAAGTTTGGATCTAGAT+AGG | - | 50876:6592-6611 | MS.gene054845:intergenic | 30.0% | |
| CCAACAATCTAACTAACTTA+AGG | - | 50876:6997-7016 | MS.gene054845:intergenic | 30.0% | |
| CCTTAAGTTAGTTAGATTGT+TGG | + | 50876:6994-7013 | MS.gene054845:intron | 30.0% | |
| GAAATGTGAAAAATGCAAAG+GGG | + | 50876:7286-7305 | MS.gene054845:intron | 30.0% | |
| TAGCACCAGTCAATAAAATA+TGG | - | 50876:6279-6298 | MS.gene054845:intergenic | 30.0% | |
| TGCATCTAGTAATTTGAAAG+GGG | - | 50876:7112-7131 | MS.gene054845:intergenic | 30.0% | |
| ! | GCAATCCATATTTTATTGAC+TGG | + | 50876:6271-6290 | MS.gene054845:CDS | 30.0% |
| ! | GTGGTTGTATTTTATGTAGT+TGG | + | 50876:7417-7436 | MS.gene054845:intron | 30.0% |
| ! | TCTCTCTTTTAATCTAAGTC+TGG | + | 50876:6739-6758 | MS.gene054845:intron | 30.0% |
| !! | AAAATGAGTTGATAGCTACA+TGG | + | 50876:6412-6431 | MS.gene054845:intron | 30.0% |
| !! | TTTTAAGCGAACTTTGAGTT+GGG | - | 50876:6505-6524 | MS.gene054845:intergenic | 30.0% |
| !! | TTTTTAAGCGAACTTTGAGT+TGG | - | 50876:6506-6525 | MS.gene054845:intergenic | 30.0% |
| !!! | GCTTTTTTTGTTGCTTCAAT+TGG | + | 50876:6943-6962 | MS.gene054845:intron | 30.0% |
| AATGTGAAAAATGCAAAGGG+GGG | + | 50876:7288-7307 | MS.gene054845:intron | 35.0% | |
| AGTTAACGTCATATTTACGG+TGG | + | 50876:6450-6469 | MS.gene054845:intron | 35.0% | |
| CAAATGGGTGGTTACATTAA+GGG | - | 50876:7063-7082 | MS.gene054845:intergenic | 35.0% | |
| GCATCTAGTAATTTGAAAGG+GGG | - | 50876:7111-7130 | MS.gene054845:intergenic | 35.0% | |
| TAGAAACATCCTCGTATTGA+CGG | + | 50876:6548-6567 | MS.gene054845:intron | 35.0% | |
| TCAAATGGGTGGTTACATTA+AGG | - | 50876:7064-7083 | MS.gene054845:intergenic | 35.0% | |
| TCATATTTACGGTGGATCTT+GGG | + | 50876:6458-6477 | MS.gene054845:intron | 35.0% | |
| TTGAAAGGGGGTTACATTTA+GGG | - | 50876:7099-7118 | MS.gene054845:intergenic | 35.0% | |
| TTTCGCTCATGTATGATTGT+AGG | - | 50876:6651-6670 | MS.gene054845:intergenic | 35.0% | |
| TTTGAAAGGGGGTTACATTT+AGG | - | 50876:7100-7119 | MS.gene054845:intergenic | 35.0% | |
| ! | AGCAATGCATTGATGAGATA+AGG | - | 50876:6160-6179 | MS.gene054845:intergenic | 35.0% |
| ! | ATTCGCATTTTCTTCTCATC+AGG | - | 50876:6112-6131 | MS.gene054845:intergenic | 35.0% |
| ! | CAATACGAGGATGTTTCTAA+TGG | - | 50876:6547-6566 | MS.gene054845:intergenic | 35.0% |
| ! | TTTTAACCAAAGACCTAGAG+AGG | - | 50876:6878-6897 | MS.gene054845:intergenic | 35.0% |
| !! | TCAACTAAGCTGATTTTCGT+TGG | + | 50876:6616-6635 | MS.gene054845:intron | 35.0% |
| !! | TTTAAGCGAACTTTGAGTTG+GGG | - | 50876:6504-6523 | MS.gene054845:intergenic | 35.0% |
| ATGTCCACCACATGAATTGT+AGG | - | 50876:6912-6931 | MS.gene054845:intergenic | 40.0% | |
| CATATTTACGGTGGATCTTG+GGG | + | 50876:6459-6478 | MS.gene054845:intron | 40.0% | |
| CCGATCTCATCATCAATTAG+CGG | - | 50876:6973-6992 | MS.gene054845:intergenic | 40.0% | |
| CTAGAGAGGAGTAAATACCT+AGG | - | 50876:6864-6883 | MS.gene054845:intergenic | 40.0% | |
| CTAGGTATTTACTCCTCTCT+AGG | + | 50876:6862-6881 | MS.gene054845:intron | 40.0% | |
| CTTCATAAAGTCGACGACAA+AGG | - | 50876:6679-6698 | MS.gene054845:intergenic | 40.0% | |
| GCTTCTTGAGAACTATTTGC+AGG | - | 50876:6253-6272 | MS.gene054845:intergenic | 40.0% | |
| GTCATATTTACGGTGGATCT+TGG | + | 50876:6457-6476 | MS.gene054845:intron | 40.0% | |
| TTAGGTGCCTACAATTCATG+TGG | + | 50876:6902-6921 | MS.gene054845:intron | 40.0% | |
| TTTACTCCTCTCTAGGTCTT+TGG | + | 50876:6869-6888 | MS.gene054845:intron | 40.0% | |
| ! | TGATCTGATGGTGTAGACTT+GGG | + | 50876:6837-6856 | MS.gene054845:intron | 40.0% |
| ! | TTGATCTGATGGTGTAGACT+TGG | + | 50876:6836-6855 | MS.gene054845:intron | 40.0% |
| !! | CCGCTAATTGATGATGAGAT+CGG | + | 50876:6970-6989 | MS.gene054845:intron | 40.0% |
| AATGGGGATACAGAGCAAGA+TGG | + | 50876:7456-7475 | MS.gene054845:CDS | 45.0% | |
| ACACCATCAGATCAACCCAT+GGG | - | 50876:6831-6850 | MS.gene054845:intergenic | 45.0% | |
| GCAAAGGGGGGTTACATTAA+GGG | + | 50876:7300-7319 | MS.gene054845:intron | 45.0% | |
| TACACCATCAGATCAACCCA+TGG | - | 50876:6832-6851 | MS.gene054845:intergenic | 45.0% | |
| TGCAAAGGGGGGTTACATTA+AGG | + | 50876:7299-7318 | MS.gene054845:intron | 45.0% | |
| TGGGTGGTTACATTAAGGGA+CGG | - | 50876:7059-7078 | MS.gene054845:intergenic | 45.0% | |
| TGGTTACATTAAGGGACGGA+GGG | - | 50876:7055-7074 | MS.gene054845:intergenic | 45.0% | |
| ! | AAACATCCTCGTATTGACGG+TGG | + | 50876:6551-6570 | MS.gene054845:intron | 45.0% |
| ! | AACATCCTCGTATTGACGGT+GGG | + | 50876:6552-6571 | MS.gene054845:intron | 45.0% |
| ! | TACCTGGGGTCCATTGATTA+TGG | - | 50876:6335-6354 | MS.gene054845:intergenic | 45.0% |
| !! | GTGGTGGACATGTTCATACA+AGG | + | 50876:6921-6940 | MS.gene054845:intron | 45.0% |
| AGGGGGGTTACATTAAGGGA+CGG | + | 50876:7304-7323 | MS.gene054845:intron | 50.0% | |
| ATGGTGTAGACTTGGGACCT+AGG | + | 50876:6844-6863 | MS.gene054845:intron | 50.0% | |
| CAAAACCCACCGTCAATACG+AGG | - | 50876:6560-6579 | MS.gene054845:intergenic | 50.0% | |
| CACCATCAGATCAACCCATG+GGG | - | 50876:6830-6849 | MS.gene054845:intergenic | 50.0% | |
| GGGTTACATTAAGGGACGGA+GGG | + | 50876:7308-7327 | MS.gene054845:intron | 50.0% | |
| GGTGCCTACAATTCATGTGG+TGG | + | 50876:6905-6924 | MS.gene054845:intron | 50.0% | |
| GTCCATAATCAATGGACCCC+AGG | + | 50876:6330-6349 | MS.gene054845:CDS | 50.0% | |
| GTGAGACAGATGCGTGCAAT+GGG | + | 50876:7439-7458 | MS.gene054845:CDS | 50.0% | |
| GTGGTTACATTAAGGGACGG+AGG | - | 50876:7056-7075 | MS.gene054845:intergenic | 50.0% | |
| TGAGACAGATGCGTGCAATG+GGG | + | 50876:7440-7459 | MS.gene054845:CDS | 50.0% | |
| !! | ATCCCCATGGGTTGATCTGA+TGG | + | 50876:6825-6844 | MS.gene054845:intron | 50.0% |
| GGGGTTACATTAAGGGACGG+AGG | + | 50876:7307-7326 | MS.gene054845:intron | 55.0% | |
| GGTGAGACAGATGCGTGCAA+TGG | + | 50876:7438-7457 | MS.gene054845:CDS | 55.0% | |
| TGAGACTGCAACGAGACGCT+TGG | + | 50876:6189-6208 | MS.gene054845:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50876 | gene | 6070 | 7481 | 6070 | ID=MS.gene054845 |
| 50876 | mRNA | 6070 | 7481 | 6070 | ID=MS.gene054845.t1;Parent=MS.gene054845 |
| 50876 | exon | 6070 | 6351 | 6070 | ID=MS.gene054845.t1.exon1;Parent=MS.gene054845.t1 |
| 50876 | CDS | 6070 | 6351 | 6070 | ID=cds.MS.gene054845.t1;Parent=MS.gene054845.t1 |
| 50876 | exon | 7436 | 7481 | 7436 | ID=MS.gene054845.t1.exon2;Parent=MS.gene054845.t1 |
| 50876 | CDS | 7436 | 7481 | 7436 | ID=cds.MS.gene054845.t1;Parent=MS.gene054845.t1 |
| Gene Sequence |
| Protein sequence |
