Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054846.t1 | XP_003607052.1 | 78 | 109 | 24 | 0 | 1 | 109 | 1 | 109 | 3.90E-39 | 170.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054846.t1 | F4JZT3 | 30.3 | 76 | 53 | 0 | 33 | 108 | 18 | 93 | 1.2e-07 | 57.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054846.t1 | G7JJU6 | 78.0 | 109 | 24 | 0 | 1 | 109 | 1 | 109 | 2.8e-39 | 170.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene054846.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054845 | MS.gene054846 | 0.960903 | 4.37E-119 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054846.t1 | MTR_6g008860 | 77.982 | 109 | 24 | 0 | 1 | 109 | 1 | 109 | 7.53e-59 | 178 |
| MS.gene054846.t1 | MTR_4g071650 | 77.982 | 109 | 24 | 0 | 1 | 109 | 1 | 109 | 7.53e-59 | 178 |
| MS.gene054846.t1 | MTR_7g099500 | 35.065 | 77 | 50 | 0 | 33 | 109 | 11 | 87 | 6.66e-13 | 60.8 |
| MS.gene054846.t1 | MTR_8g063580 | 35.065 | 77 | 50 | 0 | 33 | 109 | 12 | 88 | 2.15e-12 | 59.7 |
| MS.gene054846.t1 | MTR_7g098910 | 29.412 | 85 | 60 | 0 | 25 | 109 | 14 | 98 | 9.42e-11 | 55.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene054846.t1 | AT5G26594 | 30.263 | 76 | 53 | 0 | 33 | 108 | 18 | 93 | 3.60e-11 | 56.6 |
Find 0 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGTTAGTAGTATTAATTAA+GGG | - | 50878:6385-6404 | MS.gene054846:intergenic | 15.0% |
| !! | AAAAGTTAACGTCATATTTA+CGG | + | 50878:5672-5691 | MS.gene054846:intron | 20.0% |
| !! | AATAATTTAATAATCCCCAT+GGG | + | 50878:6039-6058 | MS.gene054846:intron | 20.0% |
| !! | ATAAAGAAATTGTTTATACC+TGG | - | 50878:5576-5595 | MS.gene054846:intergenic | 20.0% |
| !! | CAAGTTAGTAGTATTAATTA+AGG | - | 50878:6386-6405 | MS.gene054846:intergenic | 20.0% |
| !! | GGACAAAAATAAAATCAAAT+GGG | - | 50878:6304-6323 | MS.gene054846:intergenic | 20.0% |
| !! | TAAAGAAATTGTTTATACCT+GGG | - | 50878:5575-5594 | MS.gene054846:intergenic | 20.0% |
| !! | TAATAATTTAATAATCCCCA+TGG | + | 50878:6038-6057 | MS.gene054846:intron | 20.0% |
| !! | TTATGAACATTACATGAAAT+GGG | - | 50878:6575-6594 | MS.gene054846:intergenic | 20.0% |
| !! | TTTAATACACATACAAACAA+AGG | + | 50878:6428-6447 | MS.gene054846:intron | 20.0% |
| !!! | TATTAATTAAGGGTATGTTT+AGG | - | 50878:6375-6394 | MS.gene054846:intergenic | 20.0% |
| ! | AAAGAAATTGTTTATACCTG+GGG | - | 50878:5574-5593 | MS.gene054846:intergenic | 25.0% |
| ! | AAGAAATGTGAAAAATGCAA+AGG | + | 50878:6510-6529 | MS.gene054846:intron | 25.0% |
| ! | AATGCATCTAGTAATTTGAA+AGG | - | 50878:6340-6359 | MS.gene054846:intergenic | 25.0% |
| ! | ACTACCACTAAAATAAATTG+TGG | + | 50878:6624-6643 | MS.gene054846:intron | 25.0% |
| ! | AGAAATGTGAAAAATGCAAA+GGG | + | 50878:6511-6530 | MS.gene054846:intron | 25.0% |
| ! | ATAATCTGATTGTCTAGATT+CGG | - | 50878:5931-5950 | MS.gene054846:intergenic | 25.0% |
| ! | ATGCATCTAGTAATTTGAAA+GGG | - | 50878:6339-6358 | MS.gene054846:intergenic | 25.0% |
| ! | CAAAAATAAAATCAAATGGG+TGG | - | 50878:6301-6320 | MS.gene054846:intergenic | 25.0% |
| ! | CTCTTTCATTTGTTTATATC+TGG | - | 50878:5314-5333 | MS.gene054846:intergenic | 25.0% |
| ! | GGGACAAAAATAAAATCAAA+TGG | - | 50878:6305-6324 | MS.gene054846:intergenic | 25.0% |
| ! | GTCTTTGGTTAAAATCATTT+AGG | + | 50878:6110-6129 | MS.gene054846:intron | 25.0% |
| ! | GTTATGAACATTACATGAAA+TGG | - | 50878:6576-6595 | MS.gene054846:intergenic | 25.0% |
| ! | TTTCATTTGTTTATATCTGG+TGG | - | 50878:5311-5330 | MS.gene054846:intergenic | 25.0% |
| !!! | ACAACCACAATTTATTTTAG+TGG | - | 50878:6631-6650 | MS.gene054846:intergenic | 25.0% |
| !!! | TAGTTGATAAGCATTAAGTT+TGG | - | 50878:5829-5848 | MS.gene054846:intergenic | 25.0% |
| !!! | TTTGATTTGTCCATAATCAA+TGG | + | 50878:5547-5566 | MS.gene054846:CDS | 25.0% |
| AAATGTGAAAAATGCAAAGG+GGG | + | 50878:6513-6532 | MS.gene054846:intron | 30.0% | |
| ATCAATGCATTGCTAATAGA+TGG | + | 50878:5391-5410 | MS.gene054846:CDS | 30.0% | |
| CATCAAATGCTTGAATCAAT+AGG | + | 50878:5439-5458 | MS.gene054846:CDS | 30.0% | |
| CATTAAGTTTGGATCTAGAT+AGG | - | 50878:5818-5837 | MS.gene054846:intergenic | 30.0% | |
| CCAACAATCTAACTAACTTA+AGG | - | 50878:6223-6242 | MS.gene054846:intergenic | 30.0% | |
| CCTTAAGTTAGTTAGATTGT+TGG | + | 50878:6220-6239 | MS.gene054846:intron | 30.0% | |
| GAAATGTGAAAAATGCAAAG+GGG | + | 50878:6512-6531 | MS.gene054846:intron | 30.0% | |
| TAGCACCAGTCAATAAAATA+TGG | - | 50878:5504-5523 | MS.gene054846:intergenic | 30.0% | |
| TGCATCTAGTAATTTGAAAG+GGG | - | 50878:6338-6357 | MS.gene054846:intergenic | 30.0% | |
| ! | GCAATCCATATTTTATTGAC+TGG | + | 50878:5496-5515 | MS.gene054846:CDS | 30.0% |
| ! | GTGGTTGTATTTTATGTAGT+TGG | + | 50878:6643-6662 | MS.gene054846:intron | 30.0% |
| ! | TCTCTCTTTTAATCTAAGTC+TGG | + | 50878:5965-5984 | MS.gene054846:intron | 30.0% |
| !! | AAAATGAGTTGATAGCTACA+TGG | + | 50878:5637-5656 | MS.gene054846:intron | 30.0% |
| !! | TTTTAAGCGAACTTTGAGTT+GGG | - | 50878:5730-5749 | MS.gene054846:intergenic | 30.0% |
| !! | TTTTTAAGCGAACTTTGAGT+TGG | - | 50878:5731-5750 | MS.gene054846:intergenic | 30.0% |
| !!! | GCTTTTTTTGTTGCTTCAAT+TGG | + | 50878:6169-6188 | MS.gene054846:intron | 30.0% |
| AATGTGAAAAATGCAAAGGG+GGG | + | 50878:6514-6533 | MS.gene054846:intron | 35.0% | |
| AGTTAACGTCATATTTACGG+TGG | + | 50878:5675-5694 | MS.gene054846:intron | 35.0% | |
| CAAATGGGTGGTTACATTAA+GGG | - | 50878:6289-6308 | MS.gene054846:intergenic | 35.0% | |
| GCATCTAGTAATTTGAAAGG+GGG | - | 50878:6337-6356 | MS.gene054846:intergenic | 35.0% | |
| TAGAAACATCCTCGTATTGA+CGG | + | 50878:5774-5793 | MS.gene054846:intron | 35.0% | |
| TCAAATGGGTGGTTACATTA+AGG | - | 50878:6290-6309 | MS.gene054846:intergenic | 35.0% | |
| TCATATTTACGGTGGATCTT+GGG | + | 50878:5683-5702 | MS.gene054846:intron | 35.0% | |
| TTGAAAGGGGGTTACATTTA+GGG | - | 50878:6325-6344 | MS.gene054846:intergenic | 35.0% | |
| TTTCGCTCATGTATGATTGT+AGG | - | 50878:5877-5896 | MS.gene054846:intergenic | 35.0% | |
| TTTGAAAGGGGGTTACATTT+AGG | - | 50878:6326-6345 | MS.gene054846:intergenic | 35.0% | |
| ! | AGCAATGCATTGATGAGATA+AGG | - | 50878:5385-5404 | MS.gene054846:intergenic | 35.0% |
| ! | ATTCGCATTTTCTTCTCATC+AGG | - | 50878:5337-5356 | MS.gene054846:intergenic | 35.0% |
| ! | CAATACGAGGATGTTTCTAA+TGG | - | 50878:5773-5792 | MS.gene054846:intergenic | 35.0% |
| ! | TTTTAACCAAAGACCTAGAG+AGG | - | 50878:6104-6123 | MS.gene054846:intergenic | 35.0% |
| !! | TCAACTAAGCTGATTTTCGT+TGG | + | 50878:5842-5861 | MS.gene054846:intron | 35.0% |
| !! | TTTAAGCGAACTTTGAGTTG+GGG | - | 50878:5729-5748 | MS.gene054846:intergenic | 35.0% |
| ATGTCCACCACATGAATTGT+AGG | - | 50878:6138-6157 | MS.gene054846:intergenic | 40.0% | |
| CATATTTACGGTGGATCTTG+GGG | + | 50878:5684-5703 | MS.gene054846:intron | 40.0% | |
| CCGATCTCATCATCAATTAG+CGG | - | 50878:6199-6218 | MS.gene054846:intergenic | 40.0% | |
| CTAGAGAGGAGTAAATACCT+AGG | - | 50878:6090-6109 | MS.gene054846:intergenic | 40.0% | |
| CTAGGTATTTACTCCTCTCT+AGG | + | 50878:6088-6107 | MS.gene054846:intron | 40.0% | |
| CTTCATAAAGTCGACGACAA+AGG | - | 50878:5905-5924 | MS.gene054846:intergenic | 40.0% | |
| GCTTCTTGAGAACTATTTGC+AGG | - | 50878:5478-5497 | MS.gene054846:intergenic | 40.0% | |
| GTCATATTTACGGTGGATCT+TGG | + | 50878:5682-5701 | MS.gene054846:intron | 40.0% | |
| TTAGGTGCCTACAATTCATG+TGG | + | 50878:6128-6147 | MS.gene054846:intron | 40.0% | |
| TTTACTCCTCTCTAGGTCTT+TGG | + | 50878:6095-6114 | MS.gene054846:intron | 40.0% | |
| ! | TGATCTGATGGTGTAGACTT+GGG | + | 50878:6063-6082 | MS.gene054846:intron | 40.0% |
| ! | TTGATCTGATGGTGTAGACT+TGG | + | 50878:6062-6081 | MS.gene054846:intron | 40.0% |
| !! | CCGCTAATTGATGATGAGAT+CGG | + | 50878:6196-6215 | MS.gene054846:intron | 40.0% |
| AATGGGGATACAGAGCAAGA+TGG | + | 50878:6682-6701 | MS.gene054846:CDS | 45.0% | |
| ACACCATCAGATCAACCCAT+GGG | - | 50878:6057-6076 | MS.gene054846:intergenic | 45.0% | |
| GCAAAGGGGGGTTACATTAA+GGG | + | 50878:6526-6545 | MS.gene054846:intron | 45.0% | |
| TACACCATCAGATCAACCCA+TGG | - | 50878:6058-6077 | MS.gene054846:intergenic | 45.0% | |
| TGCAAAGGGGGGTTACATTA+AGG | + | 50878:6525-6544 | MS.gene054846:intron | 45.0% | |
| TGGGTGGTTACATTAAGGGA+CGG | - | 50878:6285-6304 | MS.gene054846:intergenic | 45.0% | |
| TGGTTACATTAAGGGACGGA+GGG | - | 50878:6281-6300 | MS.gene054846:intergenic | 45.0% | |
| ! | AAACATCCTCGTATTGACGG+TGG | + | 50878:5777-5796 | MS.gene054846:intron | 45.0% |
| ! | AACATCCTCGTATTGACGGT+GGG | + | 50878:5778-5797 | MS.gene054846:intron | 45.0% |
| ! | TACCTGGGGTCCATTGATTA+TGG | - | 50878:5560-5579 | MS.gene054846:intergenic | 45.0% |
| !! | GTGGTGGACATGTTCATACA+AGG | + | 50878:6147-6166 | MS.gene054846:intron | 45.0% |
| AGGGGGGTTACATTAAGGGA+CGG | + | 50878:6530-6549 | MS.gene054846:intron | 50.0% | |
| ATGGTGTAGACTTGGGACCT+AGG | + | 50878:6070-6089 | MS.gene054846:intron | 50.0% | |
| CAAAACCCACCGTCAATACG+AGG | - | 50878:5786-5805 | MS.gene054846:intergenic | 50.0% | |
| CACCATCAGATCAACCCATG+GGG | - | 50878:6056-6075 | MS.gene054846:intergenic | 50.0% | |
| GGGTTACATTAAGGGACGGA+GGG | + | 50878:6534-6553 | MS.gene054846:intron | 50.0% | |
| GGTGCCTACAATTCATGTGG+TGG | + | 50878:6131-6150 | MS.gene054846:intron | 50.0% | |
| GTCCATAATCAATGGACCCC+AGG | + | 50878:5555-5574 | MS.gene054846:CDS | 50.0% | |
| GTGAGACAGATGCGTGCAAT+GGG | + | 50878:6665-6684 | MS.gene054846:CDS | 50.0% | |
| GTGGTTACATTAAGGGACGG+AGG | - | 50878:6282-6301 | MS.gene054846:intergenic | 50.0% | |
| TGAGACAGATGCGTGCAATG+GGG | + | 50878:6666-6685 | MS.gene054846:CDS | 50.0% | |
| !! | ATCCCCATGGGTTGATCTGA+TGG | + | 50878:6051-6070 | MS.gene054846:intron | 50.0% |
| GGGGTTACATTAAGGGACGG+AGG | + | 50878:6533-6552 | MS.gene054846:intron | 55.0% | |
| GGTGAGACAGATGCGTGCAA+TGG | + | 50878:6664-6683 | MS.gene054846:CDS | 55.0% | |
| TGAGACTGCAACGAGACGCT+TGG | + | 50878:5414-5433 | MS.gene054846:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50878 | gene | 5295 | 6707 | 5295 | ID=MS.gene054846 |
| 50878 | mRNA | 5295 | 6707 | 5295 | ID=MS.gene054846.t1;Parent=MS.gene054846 |
| 50878 | exon | 5295 | 5576 | 5295 | ID=MS.gene054846.t1.exon1;Parent=MS.gene054846.t1 |
| 50878 | CDS | 5295 | 5576 | 5295 | ID=cds.MS.gene054846.t1;Parent=MS.gene054846.t1 |
| 50878 | exon | 6662 | 6707 | 6662 | ID=MS.gene054846.t1.exon2;Parent=MS.gene054846.t1 |
| 50878 | CDS | 6662 | 6707 | 6662 | ID=cds.MS.gene054846.t1;Parent=MS.gene054846.t1 |
| Gene Sequence |
| Protein sequence |
