AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene055631

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055631.t1 MTR_4g064005 96.000 150 4 1 1 150 1 148 1.22e-96 275
MS.gene055631.t1 MTR_2g096610 88.667 150 15 1 1 150 1 148 4.82e-84 243
MS.gene055631.t1 MTR_4g064967 95.333 150 5 1 1 150 1 148 1.11e-82 240
MS.gene055631.t1 MTR_4g071150 93.421 152 8 1 1 150 1 152 3.75e-82 238
MS.gene055631.t1 MTR_4g063410 93.333 150 8 1 1 150 1 148 3.39e-81 236
MS.gene055631.t1 MTR_4g063280 92.424 132 9 1 20 150 14 145 2.23e-80 234
MS.gene055631.t1 MTR_2g096570 88.961 154 12 2 1 150 1 153 9.53e-79 230
MS.gene055631.t1 MTR_8g086640 97.727 132 3 0 1 132 1 132 7.80e-76 223
MS.gene055631.t1 MTR_7g113160 78.947 133 27 1 10 142 13 144 6.57e-70 207
MS.gene055631.t1 MTR_7g108320 75.591 127 25 3 4 129 6 127 6.79e-60 182
MS.gene055631.t1 MTR_7g114060 74.803 127 28 1 3 129 2 124 6.96e-60 182
MS.gene055631.t1 MTR_7g114040 74.803 127 28 1 3 129 2 124 6.96e-60 182
MS.gene055631.t1 MTR_7g093910 73.016 126 31 1 4 129 5 127 3.32e-59 180
MS.gene055631.t1 MTR_2g082510 74.790 119 30 0 11 129 3 121 1.71e-58 178
MS.gene055631.t1 MTR_2g082220 73.950 119 31 0 11 129 3 121 1.29e-57 176
MS.gene055631.t1 MTR_6g005150 63.780 127 32 3 24 149 26 139 2.09e-47 150
MS.gene055631.t1 MTR_7g114050 68.333 120 23 3 17 132 10 118 1.56e-45 145
MS.gene055631.t1 MTR_4g063300 75.238 105 21 1 45 149 10 109 1.88e-45 144
MS.gene055631.t1 MTR_1g011750 59.292 113 44 2 21 132 23 134 8.77e-36 120
MS.gene055631.t1 MTR_1g011850 59.292 113 44 2 21 132 23 134 8.77e-36 120
MS.gene055631.t1 MTR_1g114050 59.633 109 42 2 21 128 20 127 9.02e-35 118
MS.gene055631.t1 MTR_1g071040 61.538 104 38 2 26 128 28 130 4.52e-34 116
MS.gene055631.t1 MTR_1g069770 48.958 96 48 1 45 139 9 104 1.13e-24 91.7
MS.gene055631.t1 MTR_7g010440 70.588 51 15 0 78 128 122 172 1.34e-16 74.7
MS.gene055631.t1 MTR_4g005910 74.419 43 11 0 87 129 4 46 2.34e-16 68.9
MS.gene055631.t1 MTR_4g048490 43.810 105 30 2 24 128 26 101 4.82e-16 69.7
MS.gene055631.t1 MTR_7g027610 41.818 110 38 3 20 128 10 94 5.00e-15 67.0
MS.gene055631.t1 MTR_0653s0030 36.364 99 53 2 56 146 44 140 1.18e-11 59.3
MS.gene055631.t1 MTR_2g096590 70.000 40 12 0 110 149 12 51 2.32e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055631.t1 AT5G02560 81.046 153 26 1 1 150 1 153 6.80e-78 228
MS.gene055631.t1 AT5G27670 75.000 152 34 3 1 150 1 150 4.82e-73 216
MS.gene055631.t1 AT5G02560 70.056 177 26 2 1 150 1 177 6.07e-73 216
MS.gene055631.t1 AT5G59870 74.324 148 37 1 4 150 3 150 3.42e-71 211
MS.gene055631.t1 AT1G51060 71.318 129 37 0 11 139 3 131 6.59e-61 184
MS.gene055631.t1 AT4G27230 70.543 129 38 0 11 139 3 131 2.71e-60 182
MS.gene055631.t1 AT4G27230 70.543 129 38 0 11 139 3 131 2.71e-60 182
MS.gene055631.t1 AT3G20670 71.318 129 37 0 11 139 3 131 6.43e-60 182
MS.gene055631.t1 AT5G54640 72.000 125 35 0 11 135 3 127 1.88e-58 178
MS.gene055631.t1 AT1G08880 72.222 126 32 1 7 129 2 127 2.26e-58 178
MS.gene055631.t1 AT1G54690 73.438 128 28 3 3 129 5 127 8.98e-58 177
MS.gene055631.t1 AT2G38810 60.550 109 41 2 21 128 25 132 5.84e-36 121
MS.gene055631.t1 AT2G38810 60.550 109 41 2 21 128 25 132 5.84e-36 121
MS.gene055631.t1 AT2G38810 60.550 109 41 2 21 128 25 132 5.84e-36 121
MS.gene055631.t1 AT2G38810 60.550 109 41 2 21 128 25 132 5.84e-36 121
MS.gene055631.t1 AT1G52740 61.905 105 38 2 25 128 27 130 1.58e-34 117
MS.gene055631.t1 AT3G54560 57.798 109 44 2 25 132 29 136 9.75e-34 115
MS.gene055631.t1 AT3G54560 57.798 109 44 2 25 132 29 136 9.75e-34 115
MS.gene055631.t1 AT4G13570 47.826 92 46 2 32 122 28 118 6.27e-20 80.1
MS.gene055631.t1 AT4G13570 47.253 91 46 2 33 122 35 124 1.61e-19 79.0

Find 45 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGACTTTCCAGCCTTCTT+TGG 0.239817 4.4:+41325454 None:intergenic
AATCAGATCTAAGCCTTCTT+TGG 0.251178 4.4:+41325433 None:intergenic
CTTGTCACCGACTTCTTCCT+TGG 0.333455 4.4:+41325814 None:intergenic
CGCTGCTGAAGTTCTTGAAT+TGG 0.356335 4.4:-41325668 MS.gene055631:CDS
AAAAGGATAGGGTTAATATT+AGG 0.370442 4.4:+41325517 None:intergenic
TTCTTTGGCTCTTTGTTAGC+AGG 0.375405 4.4:+41325469 None:intergenic
AGAACTGCTGCTAGGTATAC+TGG 0.389575 4.4:+41325700 None:intergenic
CAATTTCCAGTCGGAAGAAT+CGG 0.409724 4.4:-41325772 MS.gene055631:CDS
TTTCATTCTTCTTAGGCAAA+AGG 0.420546 4.4:+41325500 None:intergenic
GAAGATATGCTCAGCGTGTC+GGG 0.426128 4.4:-41325732 MS.gene055631:CDS
GGCATGTGTTGTTGGCTGTT+AGG 0.442957 4.4:-41325600 MS.gene055631:CDS
TCCTCCTCCTTTCCTTCCAC+CGG 0.452471 4.4:+41325837 None:intergenic
GCTGAAGTTCTTGAATTGGC+TGG 0.461152 4.4:-41325664 MS.gene055631:CDS
AACAAAGAGCCAAAGAAGGC+TGG 0.466354 4.4:-41325463 MS.gene055631:CDS
CATCCCAAGGCATGTGTTGT+TGG 0.466727 4.4:-41325608 MS.gene055631:CDS
AAGGGAGCCGGTGGAAGGAA+AGG 0.471425 4.4:-41325844 MS.gene055631:CDS
ACAGCCAACAACACATGCCT+TGG 0.483615 4.4:+41325604 None:intergenic
TTCTTCTTAGGCAAAAGGAT+AGG 0.488209 4.4:+41325505 None:intergenic
TCTTCTTAGGCAAAAGGATA+GGG 0.492139 4.4:+41325506 None:intergenic
TATGCTCAGCGTGTCGGGAC+AGG 0.494100 4.4:-41325727 MS.gene055631:CDS
GAGCTTGGTAAATTACTTGC+TGG 0.499780 4.4:-41325568 MS.gene055631:CDS
GCCGGTGGAAGGAAAGGAGG+AGG 0.517219 4.4:-41325838 MS.gene055631:CDS
ATCGGTCGATACTTGAAGAA+AGG 0.519021 4.4:-41325754 MS.gene055631:CDS
AAGCACGAAGACTACAAAGA+AGG 0.525553 4.4:-41325863 MS.gene055631:CDS
GGCTGGAAAGTCTCCAAAGA+AGG 0.525629 4.4:-41325446 MS.gene055631:CDS
GGAAGATATGCTCAGCGTGT+CGG 0.531969 4.4:-41325733 MS.gene055631:CDS
AGCACGAAGACTACAAAGAA+GGG 0.536534 4.4:-41325862 MS.gene055631:CDS
CAGCCAACAACACATGCCTT+GGG 0.536965 4.4:+41325605 None:intergenic
TGCTAACAAAGAGCCAAAGA+AGG 0.544648 4.4:-41325467 MS.gene055631:CDS
TCCGACTGGAAATTGAAGAC+CGG 0.546631 4.4:+41325780 None:intergenic
GGAGCCGGTGGAAGGAAAGG+AGG 0.549135 4.4:-41325841 MS.gene055631:CDS
GAAGGAAAGGAGGAGGACCA+AGG 0.555100 4.4:-41325831 MS.gene055631:CDS
GTTAGGAATGATGAAGAGCT+TGG 0.575216 4.4:-41325583 MS.gene055631:CDS
CAAAGAAGGGAGCCGGTGGA+AGG 0.583562 4.4:-41325849 MS.gene055631:CDS
TATCGACCGATTCTTCCGAC+TGG 0.586938 4.4:+41325766 None:intergenic
GATATTCAAGAACTGCTGCT+AGG 0.592671 4.4:+41325692 None:intergenic
GCTGGTGTAACCATTGCTCA+TGG 0.595329 4.4:-41325550 MS.gene055631:CDS
GTGACAAGATCAATCAGAGC+CGG 0.601599 4.4:-41325799 MS.gene055631:CDS
AAGACTACAAAGAAGGGAGC+CGG 0.606386 4.4:-41325856 MS.gene055631:CDS
AGGAGGACCAAGGAAGAAGT+CGG 0.610829 4.4:-41325821 MS.gene055631:CDS
GCCGGTCTTCAATTTCCAGT+CGG 0.613739 4.4:-41325781 MS.gene055631:CDS
AAGAACACCACCATGAGCAA+TGG 0.624982 4.4:+41325540 None:intergenic
ACTACAAAGAAGGGAGCCGG+TGG 0.646916 4.4:-41325853 MS.gene055631:CDS
GGTGTAACCATTGCTCATGG+TGG 0.672016 4.4:-41325547 MS.gene055631:CDS
AGAAGAATAGAATCATCCCA+AGG 0.679264 4.4:-41325621 MS.gene055631:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAAGGATAGGGTTAATATT+AGG + chr4.4:41325798-41325817 None:intergenic 25.0%
TTTCATTCTTCTTAGGCAAA+AGG + chr4.4:41325815-41325834 None:intergenic 30.0%
!!! GCAGTTTTTTCATTCTTCTT+AGG + chr4.4:41325822-41325841 None:intergenic 30.0%
AGAAGAATAGAATCATCCCA+AGG - chr4.4:41325691-41325710 MS.gene055631:CDS 35.0%
TCTTCTTAGGCAAAAGGATA+GGG + chr4.4:41325809-41325828 None:intergenic 35.0%
TTCTTCTTAGGCAAAAGGAT+AGG + chr4.4:41325810-41325829 None:intergenic 35.0%
AAGCACGAAGACTACAAAGA+AGG - chr4.4:41325449-41325468 MS.gene055631:CDS 40.0%
AGCACGAAGACTACAAAGAA+GGG - chr4.4:41325450-41325469 MS.gene055631:CDS 40.0%
ATCGGTCGATACTTGAAGAA+AGG - chr4.4:41325558-41325577 MS.gene055631:CDS 40.0%
CAATTTCCAGTCGGAAGAAT+CGG - chr4.4:41325540-41325559 MS.gene055631:CDS 40.0%
GAGCTTGGTAAATTACTTGC+TGG - chr4.4:41325744-41325763 MS.gene055631:CDS 40.0%
GATATTCAAGAACTGCTGCT+AGG + chr4.4:41325623-41325642 None:intergenic 40.0%
GTTAGGAATGATGAAGAGCT+TGG - chr4.4:41325729-41325748 MS.gene055631:CDS 40.0%
TGCTAACAAAGAGCCAAAGA+AGG - chr4.4:41325845-41325864 MS.gene055631:CDS 40.0%
!! TTCTTTGGCTCTTTGTTAGC+AGG + chr4.4:41325846-41325865 None:intergenic 40.0%
AACAAAGAGCCAAAGAAGGC+TGG - chr4.4:41325849-41325868 MS.gene055631:CDS 45.0%
AAGAACACCACCATGAGCAA+TGG + chr4.4:41325775-41325794 None:intergenic 45.0%
AAGACTACAAAGAAGGGAGC+CGG - chr4.4:41325456-41325475 MS.gene055631:CDS 45.0%
AGAACTGCTGCTAGGTATAC+TGG + chr4.4:41325615-41325634 None:intergenic 45.0%
CGCTGCTGAAGTTCTTGAAT+TGG - chr4.4:41325644-41325663 MS.gene055631:CDS 45.0%
GCTGAAGTTCTTGAATTGGC+TGG - chr4.4:41325648-41325667 MS.gene055631:CDS 45.0%
GTGACAAGATCAATCAGAGC+CGG - chr4.4:41325513-41325532 MS.gene055631:CDS 45.0%
TCCGACTGGAAATTGAAGAC+CGG + chr4.4:41325535-41325554 None:intergenic 45.0%
ACAGCCAACAACACATGCCT+TGG + chr4.4:41325711-41325730 None:intergenic 50.0%
AGGAGGACCAAGGAAGAAGT+CGG - chr4.4:41325491-41325510 MS.gene055631:CDS 50.0%
CAGCCAACAACACATGCCTT+GGG + chr4.4:41325710-41325729 None:intergenic 50.0%
CATCCCAAGGCATGTGTTGT+TGG - chr4.4:41325704-41325723 MS.gene055631:CDS 50.0%
GAAGATATGCTCAGCGTGTC+GGG - chr4.4:41325580-41325599 MS.gene055631:CDS 50.0%
GCCGGTCTTCAATTTCCAGT+CGG - chr4.4:41325531-41325550 MS.gene055631:CDS 50.0%
GCTGGTGTAACCATTGCTCA+TGG - chr4.4:41325762-41325781 MS.gene055631:CDS 50.0%
GGAAGATATGCTCAGCGTGT+CGG - chr4.4:41325579-41325598 MS.gene055631:CDS 50.0%
GGAGACTTTCCAGCCTTCTT+TGG + chr4.4:41325861-41325880 None:intergenic 50.0%
GGCTGGAAAGTCTCCAAAGA+AGG - chr4.4:41325866-41325885 MS.gene055631:CDS 50.0%
GGTGTAACCATTGCTCATGG+TGG - chr4.4:41325765-41325784 MS.gene055631:CDS 50.0%
! GGCATGTGTTGTTGGCTGTT+AGG - chr4.4:41325712-41325731 MS.gene055631:CDS 50.0%
! TATCGACCGATTCTTCCGAC+TGG + chr4.4:41325549-41325568 None:intergenic 50.0%
!! CTTGTCACCGACTTCTTCCT+TGG + chr4.4:41325501-41325520 None:intergenic 50.0%
ACTACAAAGAAGGGAGCCGG+TGG - chr4.4:41325459-41325478 MS.gene055631:CDS 55.0%
GAAGGAAAGGAGGAGGACCA+AGG - chr4.4:41325481-41325500 MS.gene055631:CDS 55.0%
TCCTCCTCCTTTCCTTCCAC+CGG + chr4.4:41325478-41325497 None:intergenic 55.0%
AAGGGAGCCGGTGGAAGGAA+AGG - chr4.4:41325468-41325487 MS.gene055631:CDS 60.0%
CAAAGAAGGGAGCCGGTGGA+AGG - chr4.4:41325463-41325482 MS.gene055631:CDS 60.0%
TATGCTCAGCGTGTCGGGAC+AGG - chr4.4:41325585-41325604 MS.gene055631:CDS 60.0%
GCCGGTGGAAGGAAAGGAGG+AGG - chr4.4:41325474-41325493 MS.gene055631:CDS 65.0%
GGAGCCGGTGGAAGGAAAGG+AGG - chr4.4:41325471-41325490 MS.gene055631:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 41325441 41325893 41325441 ID=MS.gene055631
chr4.4 mRNA 41325441 41325893 41325441 ID=MS.gene055631.t1;Parent=MS.gene055631
chr4.4 exon 41325441 41325893 41325441 ID=MS.gene055631.t1.exon1;Parent=MS.gene055631.t1
chr4.4 CDS 41325441 41325893 41325441 ID=cds.MS.gene055631.t1;Parent=MS.gene055631.t1
Gene Sequence

>MS.gene055631

ATGGACGCAAGCACGAAGACTACAAAGAAGGGAGCCGGTGGAAGGAAAGGAGGAGGACCAAGGAAGAAGTCGGTGACAAGATCAATCAGAGCCGGTCTTCAATTTCCAGTCGGAAGAATCGGTCGATACTTGAAGAAAGGAAGATATGCTCAGCGTGTCGGGACAGGTGCACCAGTATACCTAGCAGCAGTTCTTGAATATCTCGCTGCTGAAGTTCTTGAATTGGCTGGAAATGCAGCACGTGATAACAAGAAGAATAGAATCATCCCAAGGCATGTGTTGTTGGCTGTTAGGAATGATGAAGAGCTTGGTAAATTACTTGCTGGTGTAACCATTGCTCATGGTGGTGTTCTTCCTAATATTAACCCTATCCTTTTGCCTAAGAAGAATGAAAAAACTGCTCCTGCTAACAAAGAGCCAAAGAAGGCTGGAAAGTCTCCAAAGAAGGCTTAG
Protein sequence

>MS.gene055631.t1

MDASTKTTKKGAGGRKGGGPRKKSVTRSIRAGLQFPVGRIGRYLKKGRYAQRVGTGAPVYLAAVLEYLAAEVLELAGNAARDNKKNRIIPRHVLLAVRNDEELGKLLAGVTIAHGGVLPNINPILLPKKNEKTAPANKEPKKAGKSPKKA