Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055937.t1 | RHN48027.1 | 90.6 | 149 | 13 | 1 | 1 | 148 | 33 | 181 | 2.80E-66 | 261.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055937.t1 | A0A072U1W6 | 90.6 | 149 | 13 | 1 | 1 | 148 | 4 | 152 | 2.1e-66 | 261.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055937 | MS.gene45281 | 0.800136 | 1.66E-48 | -1.69E-46 |
| MS.gene055937 | MS.gene75941 | 0.805009 | 1.64E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055937.t1 | MTR_7g094260 | 88.587 | 184 | 20 | 1 | 1 | 183 | 4 | 187 | 1.56e-108 | 308 |
| MS.gene055937.t1 | MTR_1g089890 | 41.111 | 90 | 44 | 2 | 95 | 176 | 132 | 220 | 1.04e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGTTGAGGCTTAAGTTCT+TGG | 0.252227 | 7.4:-14903560 | None:intergenic |
| ATGATAGGTAAAATTTATAA+TGG | 0.283103 | 7.4:+14903798 | MS.gene055937:CDS |
| CATGCTTTATATTTACATTT+CGG | 0.309929 | 7.4:-14903773 | None:intergenic |
| ATTGAATGATCAAAAGTAAT+CGG | 0.346897 | 7.4:-14903627 | None:intergenic |
| TTCAACCTTGACAACTGTTT+CGG | 0.364758 | 7.4:-14903742 | None:intergenic |
| ATCAGAAAGAAAGGGCAAAT+TGG | 0.365806 | 7.4:+14903695 | MS.gene055937:CDS |
| ATTAGAACTACATCAAATAT+TGG | 0.366539 | 7.4:+14904047 | MS.gene055937:CDS |
| ATCAAGATTGTTAACATAGA+AGG | 0.379072 | 7.4:+14903582 | MS.gene055937:CDS |
| AATATAAAGCATGGGATGAT+AGG | 0.395788 | 7.4:+14903783 | MS.gene055937:CDS |
| TAAGGTTGGGTTGGTGAACT+TGG | 0.400818 | 7.4:+14903827 | MS.gene055937:CDS |
| CTTGACAACTGTTTCGGACT+GGG | 0.401883 | 7.4:-14903736 | None:intergenic |
| ATATTTACATTTCGGTCGTC+AGG | 0.410829 | 7.4:-14903765 | None:intergenic |
| TGATAGGTAAAATTTATAAT+GGG | 0.435169 | 7.4:+14903799 | MS.gene055937:CDS |
| AAGTTCACCAACCCAACCTT+AGG | 0.440670 | 7.4:-14903825 | None:intergenic |
| ATAATGGGAAACCTAAGGTT+GGG | 0.458612 | 7.4:+14903814 | MS.gene055937:CDS |
| ATAGAAGGAGGAGGTGGAGA+AGG | 0.462437 | 7.4:+14903597 | MS.gene055937:CDS |
| TCTTTCTGATATTCCAACAT+GGG | 0.489792 | 7.4:-14903682 | None:intergenic |
| AACAATGTCAAACGTTGGTA+AGG | 0.490699 | 7.4:+14903926 | MS.gene055937:CDS |
| TTGAATGATCAAAAGTAATC+GGG | 0.492415 | 7.4:-14903626 | None:intergenic |
| TGTTGGAATATCAGAAAGAA+AGG | 0.493519 | 7.4:+14903686 | MS.gene055937:CDS |
| TTCTTTCTGATATTCCAACA+TGG | 0.496286 | 7.4:-14903683 | None:intergenic |
| CGAAATGTAAATATAAAGCA+TGG | 0.497495 | 7.4:+14903774 | MS.gene055937:CDS |
| TATAATGGGAAACCTAAGGT+TGG | 0.498428 | 7.4:+14903813 | MS.gene055937:CDS |
| GTTAACATAGAAGGAGGAGG+TGG | 0.500297 | 7.4:+14903591 | MS.gene055937:CDS |
| CCTTGACAACTGTTTCGGAC+TGG | 0.508321 | 7.4:-14903737 | None:intergenic |
| TTAACAATCTTGATATGTTG+AGG | 0.522345 | 7.4:-14903573 | None:intergenic |
| AATTTATAATGGGAAACCTA+AGG | 0.544448 | 7.4:+14903809 | MS.gene055937:CDS |
| GTTGGAATATCAGAAAGAAA+GGG | 0.561830 | 7.4:+14903687 | MS.gene055937:CDS |
| AAGTCTAGCTCCTTGAAAAG+AGG | 0.562579 | 7.4:+14904071 | MS.gene055937:CDS |
| TGGGAAACCTAAGGTTGGGT+TGG | 0.569621 | 7.4:+14903818 | MS.gene055937:CDS |
| ATTGGTTACTGATCCGAGGT+TGG | 0.570911 | 7.4:+14903713 | MS.gene055937:CDS |
| GTTTCGGACTGGGCCAACCT+CGG | 0.583905 | 7.4:-14903726 | None:intergenic |
| AAAGTTGCGTCACCCCATGT+TGG | 0.595175 | 7.4:+14903669 | MS.gene055937:CDS |
| CCAGTCCGAAACAGTTGTCA+AGG | 0.596187 | 7.4:+14903737 | MS.gene055937:CDS |
| CTTTCTGATATTCCAACATG+GGG | 0.596533 | 7.4:-14903681 | None:intergenic |
| GAAATGTAAATATAAAGCAT+GGG | 0.599022 | 7.4:+14903775 | MS.gene055937:CDS |
| ATTGTTAACATAGAAGGAGG+AGG | 0.618424 | 7.4:+14903588 | MS.gene055937:CDS |
| AAGATTGTTAACATAGAAGG+AGG | 0.633279 | 7.4:+14903585 | MS.gene055937:CDS |
| GCAAATTGGTTACTGATCCG+AGG | 0.634043 | 7.4:+14903709 | MS.gene055937:CDS |
| ATTAGAACAATGTCAAACGT+TGG | 0.640630 | 7.4:+14903921 | MS.gene055937:CDS |
| GAACAACACTGTTCTAGATG+AGG | 0.653258 | 7.4:+14903953 | MS.gene055937:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGATAGGTAAAATTTATAA+TGG | + | chr7.4:14903798-14903817 | MS.gene055937:CDS | 15.0% |
| !! | TGATAGGTAAAATTTATAAT+GGG | + | chr7.4:14903799-14903818 | MS.gene055937:CDS | 15.0% |
| !! | ATTAGAACTACATCAAATAT+TGG | + | chr7.4:14904047-14904066 | MS.gene055937:CDS | 20.0% |
| !! | ATTGAATGATCAAAAGTAAT+CGG | - | chr7.4:14903630-14903649 | None:intergenic | 20.0% |
| !! | CATGCTTTATATTTACATTT+CGG | - | chr7.4:14903776-14903795 | None:intergenic | 20.0% |
| !! | GAAATGTAAATATAAAGCAT+GGG | + | chr7.4:14903775-14903794 | MS.gene055937:CDS | 20.0% |
| !! | GTACAATAAAATCTGAAAAA+TGG | - | chr7.4:14904013-14904032 | None:intergenic | 20.0% |
| ! | ATCAAGATTGTTAACATAGA+AGG | + | chr7.4:14903582-14903601 | MS.gene055937:CDS | 25.0% |
| ! | CGAAATGTAAATATAAAGCA+TGG | + | chr7.4:14903774-14903793 | MS.gene055937:CDS | 25.0% |
| ! | TTAACAATCTTGATATGTTG+AGG | - | chr7.4:14903576-14903595 | None:intergenic | 25.0% |
| ! | TTGAATGATCAAAAGTAATC+GGG | - | chr7.4:14903629-14903648 | None:intergenic | 25.0% |
| !! | AATTTATAATGGGAAACCTA+AGG | + | chr7.4:14903809-14903828 | MS.gene055937:CDS | 25.0% |
| !! | GGCAAATTCTTTTAACAATA+TGG | + | chr7.4:14903974-14903993 | MS.gene055937:CDS | 25.0% |
| AAGATTGTTAACATAGAAGG+AGG | + | chr7.4:14903585-14903604 | MS.gene055937:CDS | 30.0% | |
| AATATAAAGCATGGGATGAT+AGG | + | chr7.4:14903783-14903802 | MS.gene055937:CDS | 30.0% | |
| ATTAGAACAATGTCAAACGT+TGG | + | chr7.4:14903921-14903940 | MS.gene055937:CDS | 30.0% | |
| GTTGGAATATCAGAAAGAAA+GGG | + | chr7.4:14903687-14903706 | MS.gene055937:CDS | 30.0% | |
| TCTTTCTGATATTCCAACAT+GGG | - | chr7.4:14903685-14903704 | None:intergenic | 30.0% | |
| TGTTGGAATATCAGAAAGAA+AGG | + | chr7.4:14903686-14903705 | MS.gene055937:CDS | 30.0% | |
| TTCTTTCTGATATTCCAACA+TGG | - | chr7.4:14903686-14903705 | None:intergenic | 30.0% | |
| AACAATGTCAAACGTTGGTA+AGG | + | chr7.4:14903926-14903945 | MS.gene055937:CDS | 35.0% | |
| ATATTTACATTTCGGTCGTC+AGG | - | chr7.4:14903768-14903787 | None:intergenic | 35.0% | |
| ATCAGAAAGAAAGGGCAAAT+TGG | + | chr7.4:14903695-14903714 | MS.gene055937:CDS | 35.0% | |
| ATTGTTAACATAGAAGGAGG+AGG | + | chr7.4:14903588-14903607 | MS.gene055937:CDS | 35.0% | |
| CTTTCTGATATTCCAACATG+GGG | - | chr7.4:14903684-14903703 | None:intergenic | 35.0% | |
| TATGTTGAGGCTTAAGTTCT+TGG | - | chr7.4:14903563-14903582 | None:intergenic | 35.0% | |
| TTCAACCTTGACAACTGTTT+CGG | - | chr7.4:14903745-14903764 | None:intergenic | 35.0% | |
| ! | ATAATGGGAAACCTAAGGTT+GGG | + | chr7.4:14903814-14903833 | MS.gene055937:CDS | 35.0% |
| ! | TATAATGGGAAACCTAAGGT+TGG | + | chr7.4:14903813-14903832 | MS.gene055937:CDS | 35.0% |
| ! | AAGTCTAGCTCCTTGAAAAG+AGG | + | chr7.4:14904071-14904090 | MS.gene055937:CDS | 40.0% |
| ! | GAACAACACTGTTCTAGATG+AGG | + | chr7.4:14903953-14903972 | MS.gene055937:CDS | 40.0% |
| AAGTTCACCAACCCAACCTT+AGG | - | chr7.4:14903828-14903847 | None:intergenic | 45.0% | |
| ATTGGTTACTGATCCGAGGT+TGG | + | chr7.4:14903713-14903732 | MS.gene055937:CDS | 45.0% | |
| CTTGACAACTGTTTCGGACT+GGG | - | chr7.4:14903739-14903758 | None:intergenic | 45.0% | |
| GCAAATTGGTTACTGATCCG+AGG | + | chr7.4:14903709-14903728 | MS.gene055937:CDS | 45.0% | |
| GTTAACATAGAAGGAGGAGG+TGG | + | chr7.4:14903591-14903610 | MS.gene055937:CDS | 45.0% | |
| !! | TAAGGTTGGGTTGGTGAACT+TGG | + | chr7.4:14903827-14903846 | MS.gene055937:CDS | 45.0% |
| AAAGTTGCGTCACCCCATGT+TGG | + | chr7.4:14903669-14903688 | MS.gene055937:CDS | 50.0% | |
| ATAGAAGGAGGAGGTGGAGA+AGG | + | chr7.4:14903597-14903616 | MS.gene055937:CDS | 50.0% | |
| CCAGTCCGAAACAGTTGTCA+AGG | + | chr7.4:14903737-14903756 | MS.gene055937:CDS | 50.0% | |
| CCTTGACAACTGTTTCGGAC+TGG | - | chr7.4:14903740-14903759 | None:intergenic | 50.0% | |
| TGGGAAACCTAAGGTTGGGT+TGG | + | chr7.4:14903818-14903837 | MS.gene055937:CDS | 50.0% | |
| ! | GTTTCGGACTGGGCCAACCT+CGG | - | chr7.4:14903729-14903748 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 14903546 | 14904097 | 14903546 | ID=MS.gene055937 |
| chr7.4 | mRNA | 14903546 | 14904097 | 14903546 | ID=MS.gene055937.t1;Parent=MS.gene055937 |
| chr7.4 | exon | 14903546 | 14904097 | 14903546 | ID=MS.gene055937.t1.exon1;Parent=MS.gene055937.t1 |
| chr7.4 | CDS | 14903546 | 14904097 | 14903546 | ID=cds.MS.gene055937.t1;Parent=MS.gene055937.t1 |
| Gene Sequence |
| Protein sequence |
