AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45281


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45281.t1 MTR_1g052885 99.099 111 1 0 1 111 1 111 2.77e-75 218
MS.gene45281.t1 MTR_2g089210 86.667 105 14 0 6 110 10 114 1.24e-60 181
MS.gene45281.t1 MTR_2g081580 69.444 108 33 0 6 113 10 117 5.64e-48 150
MS.gene45281.t1 MTR_8g466250 66.667 105 35 0 6 110 11 115 1.41e-44 141
MS.gene45281.t1 MTR_2g011400 60.396 101 40 0 6 106 14 114 2.61e-38 125
MS.gene45281.t1 MTR_8g027180 52.381 105 50 0 6 110 19 123 6.61e-35 117
MS.gene45281.t1 MTR_2g081300 62.637 91 33 1 6 96 5 94 7.02e-33 111
MS.gene45281.t1 MTR_2g081350 61.538 91 34 1 6 96 9 98 1.73e-31 108
MS.gene45281.t1 MTR_2g081440 61.538 91 34 1 6 96 9 98 2.38e-31 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45281.t1 AT5G54490 66.981 106 34 1 6 110 19 124 1.80e-45 144
MS.gene45281.t1 AT4G27280 66.355 107 35 1 6 111 19 125 2.02e-43 139
MS.gene45281.t1 AT2G46600 54.128 109 50 0 6 114 22 130 2.87e-38 125

Find 22 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGACTCTTCCATCAATTC+AGG 0.351816 4.3:+52602415 None:intergenic
TGATGGAAGAGTCTTGGTTT+TGG 0.354264 4.3:-52602408 MS.gene45281:CDS
AGGTGGTGAAGGTTTAATAA+AGG 0.428490 4.3:-52602638 MS.gene45281:CDS
CTGAATTGATGGAAGAGTCT+TGG 0.441368 4.3:-52602414 MS.gene45281:CDS
TTGAAGGAAGATGAACTTGT+TGG 0.457469 4.3:-52602517 MS.gene45281:CDS
TTGTTGATGGACAAAGAAAA+AGG 0.459319 4.3:-52602595 MS.gene45281:CDS
CCTAGCTTGTTTGCCATCAC+TGG 0.464938 4.3:+52602658 None:intergenic
TGAAGACTTGTTACCAGTGA+TGG 0.470070 4.3:-52602671 MS.gene45281:CDS
TTTGGGTTTACAAGATTTGA+AGG 0.473694 4.3:-52602533 MS.gene45281:CDS
TGATAACATTGGATAGTTTG+AGG 0.497485 4.3:-52602570 MS.gene45281:CDS
GAAGGTGATTTAGATAGAGA+TGG 0.504919 4.3:-52602484 MS.gene45281:CDS
TAGGTTGAGTCCTGAATTGA+TGG 0.511296 4.3:-52602425 MS.gene45281:CDS
TAACAAGTCTTCAAAACCAT+TGG 0.541959 4.3:+52602681 None:intergenic
GAAGCTCTTCAACATGAACT+TGG 0.544590 4.3:-52602379 MS.gene45281:CDS
TTAATAAAGGAGCTATGCAA+TGG 0.554583 4.3:-52602625 MS.gene45281:CDS
GCAAACAAGCTAGGTGGTGA+AGG 0.561539 4.3:-52602649 MS.gene45281:CDS
CCAGTGATGGCAAACAAGCT+AGG 0.566082 4.3:-52602658 MS.gene45281:CDS
AGATGGAGCACTTACTCAGA+TGG 0.568398 4.3:-52602467 MS.gene45281:CDS
TTATCAACAATGGCTACCAA+TGG 0.580721 4.3:-52602697 None:intergenic
CTTGTTGGTATGATGAATGA+AGG 0.583851 4.3:-52602502 MS.gene45281:CDS
ATTGTTCCTAGGAAACTACA+AGG 0.600196 4.3:+52602333 None:intergenic
GTGATGGCAAACAAGCTAGG+TGG 0.738373 4.3:-52602655 MS.gene45281:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTTTGTGTTTTGATGTTT+AGG - chr4.3:52602592-52602611 MS.gene45281:CDS 25.0%
TAACAAGTCTTCAAAACCAT+TGG + chr4.3:52602358-52602377 None:intergenic 30.0%
TGATAACATTGGATAGTTTG+AGG - chr4.3:52602466-52602485 MS.gene45281:CDS 30.0%
TTAATAAAGGAGCTATGCAA+TGG - chr4.3:52602411-52602430 MS.gene45281:CDS 30.0%
! AGAAAAAGGTGTGATAACAT+TGG - chr4.3:52602455-52602474 MS.gene45281:CDS 30.0%
! TTGTTGATGGACAAAGAAAA+AGG - chr4.3:52602441-52602460 MS.gene45281:CDS 30.0%
! TTTGGGTTTACAAGATTTGA+AGG - chr4.3:52602503-52602522 MS.gene45281:CDS 30.0%
!!! CAATGGTTTTGAATTGTTGA+TGG - chr4.3:52602428-52602447 MS.gene45281:CDS 30.0%
AGGTGGTGAAGGTTTAATAA+AGG - chr4.3:52602398-52602417 MS.gene45281:CDS 35.0%
CTTGTTGGTATGATGAATGA+AGG - chr4.3:52602534-52602553 MS.gene45281:CDS 35.0%
GAAGGTGATTTAGATAGAGA+TGG - chr4.3:52602552-52602571 MS.gene45281:CDS 35.0%
TTGAAGGAAGATGAACTTGT+TGG - chr4.3:52602519-52602538 MS.gene45281:CDS 35.0%
CAAGACTCTTCCATCAATTC+AGG + chr4.3:52602624-52602643 None:intergenic 40.0%
CTGAATTGATGGAAGAGTCT+TGG - chr4.3:52602622-52602641 MS.gene45281:CDS 40.0%
GAAGCTCTTCAACATGAACT+TGG - chr4.3:52602657-52602676 MS.gene45281:CDS 40.0%
! TAGGTTGAGTCCTGAATTGA+TGG - chr4.3:52602611-52602630 MS.gene45281:CDS 40.0%
! TGATGGAAGAGTCTTGGTTT+TGG - chr4.3:52602628-52602647 MS.gene45281:CDS 40.0%
!! TGAAGACTTGTTACCAGTGA+TGG - chr4.3:52602365-52602384 MS.gene45281:CDS 40.0%
!!! AGGAAAAACGCTGCTGTTTT+GGG - chr4.3:52602486-52602505 MS.gene45281:CDS 40.0%
! AGATGGAGCACTTACTCAGA+TGG - chr4.3:52602569-52602588 MS.gene45281:CDS 45.0%
!! GAGGAAAAACGCTGCTGTTT+TGG - chr4.3:52602485-52602504 MS.gene45281:CDS 45.0%
CCAGTGATGGCAAACAAGCT+AGG - chr4.3:52602378-52602397 MS.gene45281:CDS 50.0%
GCAAACAAGCTAGGTGGTGA+AGG - chr4.3:52602387-52602406 MS.gene45281:CDS 50.0%
GTGATGGCAAACAAGCTAGG+TGG - chr4.3:52602381-52602400 MS.gene45281:CDS 50.0%
!! CCTAGCTTGTTTGCCATCAC+TGG + chr4.3:52602381-52602400 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 52602348 52602710 52602348 ID=MS.gene45281
chr4.3 mRNA 52602348 52602710 52602348 ID=MS.gene45281.t1;Parent=MS.gene45281
chr4.3 exon 52602348 52602710 52602348 ID=MS.gene45281.t1.exon1;Parent=MS.gene45281.t1
chr4.3 CDS 52602348 52602710 52602348 ID=cds.MS.gene45281.t1;Parent=MS.gene45281.t1
Gene Sequence

>MS.gene45281

ATGGCTACCAATGGTTTTGAAGACTTGTTACCAGTGATGGCAAACAAGCTAGGTGGTGAAGGTTTAATAAAGGAGCTATGCAATGGTTTTGAATTGTTGATGGACAAAGAAAAAGGTGTGATAACATTGGATAGTTTGAGGAAAAACGCTGCTGTTTTGGGTTTACAAGATTTGAAGGAAGATGAACTTGTTGGTATGATGAATGAAGGTGATTTAGATAGAGATGGAGCACTTACTCAGATGGAGTTTTGTGTTTTGATGTTTAGGTTGAGTCCTGAATTGATGGAAGAGTCTTGGTTTTGGCTTGAAGAAGCTCTTCAACATGAACTTGGTGACAATAACAATAATAACTGTTCCTTGTAG

Protein sequence

>MS.gene45281.t1

MATNGFEDLLPVMANKLGGEGLIKELCNGFELLMDKEKGVITLDSLRKNAAVLGLQDLKEDELVGMMNEGDLDRDGALTQMEFCVLMFRLSPELMEESWFWLEEALQHELGDNNNNNCSL