Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056072.t1 | PNY00099.1 | 98.4 | 125 | 2 | 0 | 1 | 125 | 1 | 125 | 7.20E-61 | 243 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056072.t1 | Q9M9W1 | 84.0 | 125 | 19 | 1 | 1 | 125 | 1 | 124 | 1.4e-52 | 206.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056072.t1 | A0A2K3NAM8 | 98.4 | 125 | 2 | 0 | 1 | 125 | 1 | 125 | 5.2e-61 | 243.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056072 | MS.gene068924 | 0.80402 | 2.64E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056072.t1 | MTR_1g083430 | 97.600 | 125 | 3 | 0 | 1 | 125 | 1 | 125 | 2.93e-87 | 249 |
| MS.gene056072.t1 | MTR_1g088450 | 90.756 | 119 | 10 | 1 | 7 | 125 | 2 | 119 | 1.33e-64 | 192 |
| MS.gene056072.t1 | MTR_7g112770 | 93.519 | 108 | 7 | 0 | 18 | 125 | 17 | 124 | 1.84e-61 | 184 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056072.t1 | AT3G05560 | 84.000 | 125 | 19 | 1 | 1 | 125 | 1 | 124 | 1.56e-62 | 187 |
| MS.gene056072.t1 | AT3G05560 | 84.000 | 125 | 19 | 1 | 1 | 125 | 1 | 124 | 1.56e-62 | 187 |
| MS.gene056072.t1 | AT3G05560 | 84.000 | 125 | 19 | 1 | 1 | 125 | 1 | 124 | 1.56e-62 | 187 |
| MS.gene056072.t1 | AT5G27770 | 84.694 | 98 | 15 | 0 | 17 | 114 | 16 | 113 | 4.50e-49 | 153 |
| MS.gene056072.t1 | AT1G02830 | 68.644 | 118 | 34 | 2 | 1 | 116 | 1 | 117 | 2.16e-42 | 136 |
Find 28 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGATGCTGTTACTGTTTCC+AGG | 0.305504 | 1.4:+67493831 | MS.gene056072:CDS |
| GATCTTGTCTTCCACGGGTT+TGG | 0.325644 | 1.4:-67493738 | None:intergenic |
| GTTTCTTCAAGAGAGGATTA+AGG | 0.367162 | 1.4:+67493784 | MS.gene056072:CDS |
| ACTTGAAGAAACACAATGTA+AGG | 0.413403 | 1.4:+67495144 | MS.gene056072:CDS |
| CACGGGTTTGGCGCAGTCAA+TGG | 0.415262 | 1.4:-67493726 | None:intergenic |
| GTCCATGATCTTGTCTTCCA+CGG | 0.443029 | 1.4:-67493744 | None:intergenic |
| GGTGGTAAACCCGGTGCTCT+TGG | 0.483396 | 1.4:+67493809 | MS.gene056072:CDS |
| AACAGCATCACCAAGAGCAC+CGG | 0.505615 | 1.4:-67493819 | None:intergenic |
| AGGTTCAAAGGGAAAGAAGA+AGG | 0.510234 | 1.4:+67493688 | MS.gene056072:CDS |
| CTGATAGCAACTTCTCCAAA+CGG | 0.520527 | 1.4:+67493879 | MS.gene056072:CDS |
| AGAAACACAATGTAAGGGAT+TGG | 0.522187 | 1.4:+67495150 | MS.gene056072:CDS |
| TCCATGATCTTGTCTTCCAC+GGG | 0.522466 | 1.4:-67493743 | None:intergenic |
| TCGAAAAGTTTCTTCAAGAG+AGG | 0.523656 | 1.4:+67493777 | MS.gene056072:CDS |
| CAAGAGAGGATTAAGGTTGG+TGG | 0.536618 | 1.4:+67493791 | MS.gene056072:CDS |
| ATTAGATGCAGCAGGTTCAA+AGG | 0.537258 | 1.4:+67493676 | MS.gene056072:CDS |
| TTAGATGCAGCAGGTTCAAA+GGG | 0.548577 | 1.4:+67493677 | MS.gene056072:CDS |
| TTCAACATTGCTGAGAATGA+AGG | 0.551218 | 1.4:+67495224 | MS.gene056072:CDS |
| ATTAAGGTTGGTGGTAAACC+CGG | 0.555719 | 1.4:+67493800 | MS.gene056072:CDS |
| ACCCGTGGAAGACAAGATCA+TGG | 0.560910 | 1.4:+67493742 | MS.gene056072:CDS |
| CTTCAAGAGAGGATTAAGGT+TGG | 0.564429 | 1.4:+67493788 | MS.gene056072:CDS |
| ATGGGTCGATTAGATGCAGC+AGG | 0.568253 | 1.4:+67493668 | MS.gene056072:CDS |
| CAGAGTGATTGCTTCAAACA+AGG | 0.577999 | 1.4:+67495175 | MS.gene056072:CDS |
| CATTGACTGCGCCAAACCCG+TGG | 0.595779 | 1.4:+67493727 | MS.gene056072:CDS |
| ATAGAAATGTTTATGAGCTG+AGG | 0.614228 | 1.4:+67495198 | MS.gene056072:CDS |
| TGATGCTGTTACTGTTTCCA+GGG | 0.614918 | 1.4:+67493832 | MS.gene056072:CDS |
| GGTTCAAAGGGAAAGAAGAA+GGG | 0.618619 | 1.4:+67493689 | MS.gene056072:CDS |
| CTTGAAGAAACACAATGTAA+GGG | 0.629064 | 1.4:+67495145 | MS.gene056072:CDS |
| ACAGCATCACCAAGAGCACC+GGG | 0.629308 | 1.4:-67493818 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTAATTAACATTGTAG+TGG | + | chr1.4:67494191-67494210 | MS.gene056072:intron | 15.0% |
| !! | CAAAAACTTTCAAATGTTAT+TGG | + | chr1.4:67494831-67494850 | MS.gene056072:intron | 20.0% |
| !! | GAGAAAAAAAAAATGACTTT+GGG | - | chr1.4:67494598-67494617 | None:intergenic | 20.0% |
| !! | GGATAAAAAATGTGAAAAAA+AGG | - | chr1.4:67494922-67494941 | None:intergenic | 20.0% |
| !! | TGAATTTGTAAAAATCAATG+CGG | - | chr1.4:67494538-67494557 | None:intergenic | 20.0% |
| !! | TTCGCTAAAAAATAAAATAG+CGG | + | chr1.4:67494023-67494042 | MS.gene056072:intron | 20.0% |
| !!! | AGTTTATTGTTTTGTTTGAT+TGG | + | chr1.4:67493944-67493963 | MS.gene056072:intron | 20.0% |
| !!! | TAATTGATAATCGATTATGT+AGG | - | chr1.4:67494482-67494501 | None:intergenic | 20.0% |
| !!! | TTATTTTCATGAGCTTATAA+CGG | - | chr1.4:67494273-67494292 | None:intergenic | 20.0% |
| !!! | TTGAATTGTTTGATTTATGT+TGG | + | chr1.4:67493986-67494005 | MS.gene056072:intron | 20.0% |
| !!! | TTGATTTATGTTGGTTTTTA+AGG | + | chr1.4:67493995-67494014 | MS.gene056072:intron | 20.0% |
| ! | AAAAATAGAGAATAACCGTT+TGG | - | chr1.4:67493897-67493916 | None:intergenic | 25.0% |
| ! | ATGAGTTGTTAAAAAAAGCA+TGG | - | chr1.4:67495088-67495107 | None:intergenic | 25.0% |
| ! | GAATTTGTAAAAATCAATGC+GGG | - | chr1.4:67494537-67494556 | None:intergenic | 25.0% |
| ! | GGAGAAAAAAAAAATGACTT+TGG | - | chr1.4:67494599-67494618 | None:intergenic | 25.0% |
| !!! | GTTAGTTAACTTTTGATTCA+TGG | + | chr1.4:67494767-67494786 | MS.gene056072:intron | 25.0% |
| !!! | TAGAGGTATAGAATTGATTT+TGG | + | chr1.4:67494422-67494441 | MS.gene056072:intron | 25.0% |
| !!! | TTATTGTTTTGTTTGATTGG+AGG | + | chr1.4:67493947-67493966 | MS.gene056072:intron | 25.0% |
| AAGTAGCTTATAGCTTATCA+CGG | - | chr1.4:67494232-67494251 | None:intergenic | 30.0% | |
| ACTTGAAGAAACACAATGTA+AGG | + | chr1.4:67495144-67495163 | MS.gene056072:CDS | 30.0% | |
| ATAGAAATGTTTATGAGCTG+AGG | + | chr1.4:67495198-67495217 | MS.gene056072:CDS | 30.0% | |
| ATCAATTATACCTTGAAGCT+AGG | + | chr1.4:67494494-67494513 | MS.gene056072:intron | 30.0% | |
| ATGATTAAAAAAGACCCGTT+TGG | - | chr1.4:67494325-67494344 | None:intergenic | 30.0% | |
| ATTGATTATACAATCTCGCT+TGG | + | chr1.4:67494653-67494672 | MS.gene056072:intron | 30.0% | |
| CATACGAACTTATAAGCTAT+GGG | + | chr1.4:67494342-67494361 | MS.gene056072:intron | 30.0% | |
| CTTGAAGAAACACAATGTAA+GGG | + | chr1.4:67495145-67495164 | MS.gene056072:CDS | 30.0% | |
| TAAATATGACAGCTACCAAA+CGG | + | chr1.4:67494307-67494326 | MS.gene056072:intron | 30.0% | |
| TCATACGAACTTATAAGCTA+TGG | + | chr1.4:67494341-67494360 | MS.gene056072:intron | 30.0% | |
| TCATGGAATATACAATCTCT+GGG | + | chr1.4:67494784-67494803 | MS.gene056072:intron | 30.0% | |
| TTAACAACTCATATTGTTCC+AGG | + | chr1.4:67495096-67495115 | MS.gene056072:intron | 30.0% | |
| TTCATGGAATATACAATCTC+TGG | + | chr1.4:67494783-67494802 | MS.gene056072:intron | 30.0% | |
| TTCTGTCTAAATTGCAATGA+TGG | + | chr1.4:67494873-67494892 | MS.gene056072:intron | 30.0% | |
| TTGATTATACAATCTCGCTT+GGG | + | chr1.4:67494654-67494673 | MS.gene056072:intron | 30.0% | |
| ! | AATCCAAACACAGTTGATAT+TGG | + | chr1.4:67494622-67494641 | MS.gene056072:intron | 30.0% |
| ! | ATGTTATTGGCAATTTTGTG+TGG | + | chr1.4:67494844-67494863 | MS.gene056072:intron | 30.0% |
| ! | GATTGGAGGATTTATGTTTT+AGG | + | chr1.4:67493961-67493980 | MS.gene056072:intron | 30.0% |
| ! | GTGTTTCTTCAAGTACTTTT+TGG | - | chr1.4:67495138-67495157 | None:intergenic | 30.0% |
| ! | TGTTATTGGCAATTTTGTGT+GGG | + | chr1.4:67494845-67494864 | MS.gene056072:intron | 30.0% |
| !! | AGAATTGATTTTGGCATGTT+TGG | + | chr1.4:67494431-67494450 | MS.gene056072:intron | 30.0% |
| !!! | ACACTGTTTTTGTGATCTAA+AGG | + | chr1.4:67495057-67495076 | MS.gene056072:intron | 30.0% |
| AAATATGACAGCTACCAAAC+GGG | + | chr1.4:67494308-67494327 | MS.gene056072:intron | 35.0% | |
| AGAAACACAATGTAAGGGAT+TGG | + | chr1.4:67495150-67495169 | MS.gene056072:CDS | 35.0% | |
| ATCAACTGTGTTTGGATTTG+CGG | - | chr1.4:67494620-67494639 | None:intergenic | 35.0% | |
| CAACCAATATCAACTGTGTT+TGG | - | chr1.4:67494628-67494647 | None:intergenic | 35.0% | |
| CATATTGCGATGATGCTAAA+AGG | - | chr1.4:67494709-67494728 | None:intergenic | 35.0% | |
| GTTTCTTCAAGAGAGGATTA+AGG | + | chr1.4:67493784-67493803 | MS.gene056072:CDS | 35.0% | |
| TCGAAAAGTTTCTTCAAGAG+AGG | + | chr1.4:67493777-67493796 | MS.gene056072:CDS | 35.0% | |
| TTCAACATTGCTGAGAATGA+AGG | + | chr1.4:67495224-67495243 | MS.gene056072:CDS | 35.0% | |
| ! | TTTTTATCCCCTGCTTCAAA+AGG | + | chr1.4:67494933-67494952 | MS.gene056072:intron | 35.0% |
| !! | GAAACTGACATTGATTCTAG+AGG | + | chr1.4:67494405-67494424 | MS.gene056072:intron | 35.0% |
| !! | GACTTTGGGTGAATTGATTT+TGG | - | chr1.4:67494584-67494603 | None:intergenic | 35.0% |
| !! | TTAGCAGGCTTATTTGCTAT+CGG | - | chr1.4:67494098-67494117 | None:intergenic | 35.0% |
| !!! | AACAAAAGCCTTTTGAAGCA+GGG | - | chr1.4:67494944-67494963 | None:intergenic | 35.0% |
| !!! | CTATGGGCTCAAATTTTTTG+AGG | + | chr1.4:67494358-67494377 | MS.gene056072:intron | 35.0% |
| AGCTTCAAATCCTAGCTTCA+AGG | - | chr1.4:67494507-67494526 | None:intergenic | 40.0% | |
| AGGTTCAAAGGGAAAGAAGA+AGG | + | chr1.4:67493688-67493707 | MS.gene056072:CDS | 40.0% | |
| ATCAGTCTGTCTTCAACATC+TGG | + | chr1.4:67495020-67495039 | MS.gene056072:intron | 40.0% | |
| ATTAGATGCAGCAGGTTCAA+AGG | + | chr1.4:67493676-67493695 | MS.gene056072:CDS | 40.0% | |
| ATTATACAATCTCGCTTGGG+TGG | + | chr1.4:67494657-67494676 | MS.gene056072:intron | 40.0% | |
| CACTATTAACCGCTATTAGC+AGG | - | chr1.4:67494113-67494132 | None:intergenic | 40.0% | |
| CAGAGTGATTGCTTCAAACA+AGG | + | chr1.4:67495175-67495194 | MS.gene056072:CDS | 40.0% | |
| CAGTTGTTCGTATTCAGATG+TGG | + | chr1.4:67494969-67494988 | MS.gene056072:intron | 40.0% | |
| CTGATAGCAACTTCTCCAAA+CGG | + | chr1.4:67493879-67493898 | MS.gene056072:CDS | 40.0% | |
| CTGCTAATAGCGGTTAATAG+TGG | + | chr1.4:67494111-67494130 | MS.gene056072:intron | 40.0% | |
| CTTCAAGAGAGGATTAAGGT+TGG | + | chr1.4:67493788-67493807 | MS.gene056072:CDS | 40.0% | |
| GCAAATAAGCCTGCTAATAG+CGG | + | chr1.4:67494101-67494120 | MS.gene056072:intron | 40.0% | |
| GGTCAAATACTTGAGATACC+TGG | - | chr1.4:67495117-67495136 | None:intergenic | 40.0% | |
| GGTTCAAAGGGAAAGAAGAA+GGG | + | chr1.4:67493689-67493708 | MS.gene056072:CDS | 40.0% | |
| TAGCGGTTAATAGTGGTTGA+AGG | + | chr1.4:67494118-67494137 | MS.gene056072:intron | 40.0% | |
| TGATGCTGTTACTGTTTCCA+GGG | + | chr1.4:67493832-67493851 | MS.gene056072:CDS | 40.0% | |
| TTAGATGCAGCAGGTTCAAA+GGG | + | chr1.4:67493677-67493696 | MS.gene056072:CDS | 40.0% | |
| ! | AGTAATCTTGCTCTTTTCCC+TGG | - | chr1.4:67493852-67493871 | None:intergenic | 40.0% |
| !! | ATTAAGGTTGGTGGTAAACC+CGG | + | chr1.4:67493800-67493819 | MS.gene056072:CDS | 40.0% |
| !!! | ACAAAAGCCTTTTGAAGCAG+GGG | - | chr1.4:67494943-67494962 | None:intergenic | 40.0% |
| !!! | CAACAAAAGCCTTTTGAAGC+AGG | - | chr1.4:67494945-67494964 | None:intergenic | 40.0% |
| CAAGAGAGGATTAAGGTTGG+TGG | + | chr1.4:67493791-67493810 | MS.gene056072:CDS | 45.0% | |
| GTCCATGATCTTGTCTTCCA+CGG | - | chr1.4:67493747-67493766 | None:intergenic | 45.0% | |
| GTGATGCTGTTACTGTTTCC+AGG | + | chr1.4:67493831-67493850 | MS.gene056072:CDS | 45.0% | |
| TCCATGATCTTGTCTTCCAC+GGG | - | chr1.4:67493746-67493765 | None:intergenic | 45.0% | |
| ACCCGTGGAAGACAAGATCA+TGG | + | chr1.4:67493742-67493761 | MS.gene056072:CDS | 50.0% | |
| ATGGGTCGATTAGATGCAGC+AGG | + | chr1.4:67493668-67493687 | MS.gene056072:CDS | 50.0% | |
| GATCTTGTCTTCCACGGGTT+TGG | - | chr1.4:67493741-67493760 | None:intergenic | 50.0% | |
| GCTTGGGTGGTATTCTATGC+AGG | + | chr1.4:67494670-67494689 | MS.gene056072:intron | 50.0% | |
| ! | AACAGCATCACCAAGAGCAC+CGG | - | chr1.4:67493822-67493841 | None:intergenic | 50.0% |
| !! | ACAGCATCACCAAGAGCACC+GGG | - | chr1.4:67493821-67493840 | None:intergenic | 55.0% |
| CACGGGTTTGGCGCAGTCAA+TGG | - | chr1.4:67493729-67493748 | None:intergenic | 60.0% | |
| GGTGGTAAACCCGGTGCTCT+TGG | + | chr1.4:67493809-67493828 | MS.gene056072:CDS | 60.0% | |
| ! | CATTGACTGCGCCAAACCCG+TGG | + | chr1.4:67493727-67493746 | MS.gene056072:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 67493668 | 67495262 | 67493668 | ID=MS.gene056072 |
| chr1.4 | mRNA | 67493668 | 67495262 | 67493668 | ID=MS.gene056072.t1;Parent=MS.gene056072 |
| chr1.4 | exon | 67493668 | 67493900 | 67493668 | ID=MS.gene056072.t1.exon1;Parent=MS.gene056072.t1 |
| chr1.4 | CDS | 67493668 | 67493900 | 67493668 | ID=cds.MS.gene056072.t1;Parent=MS.gene056072.t1 |
| chr1.4 | exon | 67495118 | 67495262 | 67495118 | ID=MS.gene056072.t1.exon2;Parent=MS.gene056072.t1 |
| chr1.4 | CDS | 67495118 | 67495262 | 67495118 | ID=cds.MS.gene056072.t1;Parent=MS.gene056072.t1 |
| Gene Sequence |
| Protein sequence |
