AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068924


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068924.t1 MTR_2g046150 98.182 165 2 1 1 165 1 164 3.53e-116 326
MS.gene068924.t1 MTR_7g111900 46.479 142 75 1 6 146 2 143 6.06e-38 128
MS.gene068924.t1 MTR_1g084200 43.137 153 86 1 6 157 2 154 1.59e-36 124
MS.gene068924.t1 MTR_7g103200 43.382 136 76 1 6 140 2 137 2.44e-36 123
MS.gene068924.t1 MTR_2g019480 38.129 139 86 0 15 153 16 154 5.98e-32 112
MS.gene068924.t1 MTR_2g091215 40.909 132 78 0 11 142 3 134 1.06e-27 100
MS.gene068924.t1 MTR_2g091210 38.462 130 80 0 11 140 3 132 8.50e-27 98.6
MS.gene068924.t1 MTR_4g122930 36.986 146 85 4 7 147 3 146 7.18e-24 91.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068924.t1 AT4G24130 68.667 150 46 1 1 149 1 150 5.72e-72 214
MS.gene068924.t1 AT1G09310 46.207 145 76 2 7 150 3 146 1.28e-34 120
MS.gene068924.t1 AT1G56580 44.595 148 79 3 7 151 3 150 2.91e-34 118
MS.gene068924.t1 AT5G46230 37.121 132 83 0 9 140 8 139 1.87e-31 110
MS.gene068924.t1 AT1G30020 33.846 130 82 1 7 136 6 131 1.43e-25 96.3
MS.gene068924.t1 AT5G49600 34.694 147 85 4 7 143 1 146 1.36e-20 83.6

Find 33 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGGTGTTCTTCCCTTAA+AGG 0.146605 2.1:-45723731 MS.gene068924:CDS
ACATTCAACAAGATCCTTTA+AGG 0.179628 2.1:+45723717 None:intergenic
AGAGTAAGCAATTGTTTGTT+TGG 0.287883 2.1:-45723546 MS.gene068924:CDS
TTAGAGAAACTGGCTTTGTT+TGG 0.347222 2.1:-45723687 MS.gene068924:CDS
TGTGGAAGAGTTAGAGAAAC+TGG 0.366206 2.1:-45723697 MS.gene068924:CDS
TCAATACTCATTTCAACTAT+AGG 0.386964 2.1:+45723520 None:intergenic
ATTTATGAGTGTTGAGGAAA+AGG 0.388563 2.1:-45723428 MS.gene068924:CDS
CTTTAAGGGAAGAACACCTT+TGG 0.399056 2.1:+45723732 None:intergenic
GAGTAAGCAATTGTTTGTTT+GGG 0.413681 2.1:-45723545 MS.gene068924:CDS
GAAGTTGCAATTGAAGGATA+AGG 0.421440 2.1:-45723395 MS.gene068924:CDS
CATTCAACAAGATCCTTTAA+GGG 0.423103 2.1:+45723718 None:intergenic
TTTAAGGGAAGAACACCTTT+GGG 0.434347 2.1:+45723733 None:intergenic
CTTTGTTTGGATGAAACAAA+AGG 0.441279 2.1:-45723674 MS.gene068924:CDS
ATACAACTCTTAGAAGAGCT+TGG 0.448905 2.1:-45723760 MS.gene068924:CDS
TTTAAGACTCCTATGGGGAT+TGG 0.453850 2.1:-45723472 MS.gene068924:CDS
GTATGAGAAGTTGCAATTGA+AGG 0.453990 2.1:-45723401 MS.gene068924:CDS
ATAGTTGAAATGAGTATTGA+GGG 0.460794 2.1:-45723517 MS.gene068924:CDS
AAGCTCTTCTAAGAGTTGTA+TGG 0.469293 2.1:+45723762 None:intergenic
CCTTCAAAATAATGTTCACT+TGG 0.482969 2.1:+45723649 None:intergenic
TATAGTTGAAATGAGTATTG+AGG 0.501110 2.1:-45723518 MS.gene068924:CDS
ACAAAGACTCTTGTGAGCTA+TGG 0.512268 2.1:-45723625 MS.gene068924:CDS
TTAAAGGATCTTGTTGAATG+TGG 0.521582 2.1:-45723715 MS.gene068924:CDS
CTTCTAAGAGTTGTATGGAA+TGG 0.521965 2.1:+45723767 None:intergenic
CATCTTGAATTTCTCCACAT+AGG 0.539432 2.1:+45723588 None:intergenic
GTTGCAATTGAAGGATAAGG+AGG 0.546376 2.1:-45723392 MS.gene068924:CDS
GCAGGTGCAGAAATAGTGTA+TGG 0.551940 2.1:-45723808 MS.gene068924:CDS
TACTTCATTTATGAGTGTTG+AGG 0.556318 2.1:-45723434 MS.gene068924:CDS
TTCAAGATGAAGAAAATGAG+TGG 0.567086 2.1:-45723574 MS.gene068924:CDS
GAAGAGCTTGGATTTCCCAA+AGG 0.601476 2.1:-45723748 MS.gene068924:CDS
ATGGTAACAAATGAACGTGC+AGG 0.612544 2.1:-45723826 MS.gene068924:CDS
AAAGACTTACCAATCCCCAT+AGG 0.653062 2.1:+45723463 None:intergenic
AATTGAAGTCACTGCCTATG+TGG 0.667176 2.1:-45723602 MS.gene068924:CDS
AATGGCTAGCAACAGCACAA+TGG 0.699467 2.1:-45723845 MS.gene068924:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TCAATACTCATTTCAACTAT+AGG + chr2.1:45723696-45723715 None:intergenic 25.0%
!! TATAGTTGAAATGAGTATTG+AGG - chr2.1:45723695-45723714 MS.gene068924:CDS 25.0%
!!! ATAGTTGAAATGAGTATTGA+GGG - chr2.1:45723696-45723715 MS.gene068924:CDS 25.0%
!!! ATGTATTTTAAGACTCCTAT+GGG - chr2.1:45723735-45723754 MS.gene068924:CDS 25.0%
ACATTCAACAAGATCCTTTA+AGG + chr2.1:45723499-45723518 None:intergenic 30.0%
AGAGTAAGCAATTGTTTGTT+TGG - chr2.1:45723667-45723686 MS.gene068924:CDS 30.0%
CATTCAACAAGATCCTTTAA+GGG + chr2.1:45723498-45723517 None:intergenic 30.0%
CCTTCAAAATAATGTTCACT+TGG + chr2.1:45723567-45723586 None:intergenic 30.0%
GAGTAAGCAATTGTTTGTTT+GGG - chr2.1:45723668-45723687 MS.gene068924:CDS 30.0%
TTAAAGGATCTTGTTGAATG+TGG - chr2.1:45723498-45723517 MS.gene068924:CDS 30.0%
TTCAAGATGAAGAAAATGAG+TGG - chr2.1:45723639-45723658 MS.gene068924:CDS 30.0%
! CTTTGTTTGGATGAAACAAA+AGG - chr2.1:45723539-45723558 MS.gene068924:CDS 30.0%
! TACTTCATTTATGAGTGTTG+AGG - chr2.1:45723779-45723798 MS.gene068924:CDS 30.0%
!! ATTTATGAGTGTTGAGGAAA+AGG - chr2.1:45723785-45723804 MS.gene068924:CDS 30.0%
!!! ATGAGTATTGAGGGTTTTAA+TGG - chr2.1:45723705-45723724 MS.gene068924:CDS 30.0%
!!! CCAAGTGAACATTATTTTGA+AGG - chr2.1:45723564-45723583 MS.gene068924:CDS 30.0%
!!! GATGTATTTTAAGACTCCTA+TGG - chr2.1:45723734-45723753 MS.gene068924:CDS 30.0%
!!! TGTATTTTAAGACTCCTATG+GGG - chr2.1:45723736-45723755 MS.gene068924:CDS 30.0%
ATACAACTCTTAGAAGAGCT+TGG - chr2.1:45723453-45723472 MS.gene068924:CDS 35.0%
CATCTTGAATTTCTCCACAT+AGG + chr2.1:45723628-45723647 None:intergenic 35.0%
GAAGTTGCAATTGAAGGATA+AGG - chr2.1:45723818-45723837 MS.gene068924:CDS 35.0%
GTATGAGAAGTTGCAATTGA+AGG - chr2.1:45723812-45723831 MS.gene068924:CDS 35.0%
TTTAAGGGAAGAACACCTTT+GGG + chr2.1:45723483-45723502 None:intergenic 35.0%
! AAGCTCTTCTAAGAGTTGTA+TGG + chr2.1:45723454-45723473 None:intergenic 35.0%
! CTTCTAAGAGTTGTATGGAA+TGG + chr2.1:45723449-45723468 None:intergenic 35.0%
!! TTAGAGAAACTGGCTTTGTT+TGG - chr2.1:45723526-45723545 MS.gene068924:CDS 35.0%
AAAGACTTACCAATCCCCAT+AGG + chr2.1:45723753-45723772 None:intergenic 40.0%
AATTGAAGTCACTGCCTATG+TGG - chr2.1:45723611-45723630 MS.gene068924:CDS 40.0%
ACAAAGACTCTTGTGAGCTA+TGG - chr2.1:45723588-45723607 MS.gene068924:CDS 40.0%
ATGGTAACAAATGAACGTGC+AGG - chr2.1:45723387-45723406 MS.gene068924:CDS 40.0%
GTTGCAATTGAAGGATAAGG+AGG - chr2.1:45723821-45723840 MS.gene068924:CDS 40.0%
TGTGGAAGAGTTAGAGAAAC+TGG - chr2.1:45723516-45723535 MS.gene068924:CDS 40.0%
! CTTTAAGGGAAGAACACCTT+TGG + chr2.1:45723484-45723503 None:intergenic 40.0%
! TTTAAGACTCCTATGGGGAT+TGG - chr2.1:45723741-45723760 MS.gene068924:CDS 40.0%
!! CAAAGGTGTTCTTCCCTTAA+AGG - chr2.1:45723482-45723501 MS.gene068924:CDS 40.0%
GAAGAGCTTGGATTTCCCAA+AGG - chr2.1:45723465-45723484 MS.gene068924:CDS 45.0%
GCAGGTGCAGAAATAGTGTA+TGG - chr2.1:45723405-45723424 MS.gene068924:CDS 45.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 45723369 45723866 45723369 ID=MS.gene068924
chr2.1 mRNA 45723369 45723866 45723369 ID=MS.gene068924.t1;Parent=MS.gene068924
chr2.1 exon 45723369 45723866 45723369 ID=MS.gene068924.t1.exon1;Parent=MS.gene068924.t1
chr2.1 CDS 45723369 45723866 45723369 ID=cds.MS.gene068924.t1;Parent=MS.gene068924.t1
Gene Sequence

>MS.gene068924

ATGGCTAGCAACAGCACAATGGTAACAAATGAACGTGCAGGTGCAGAAATAGTGTATGGTTCTGAAGAATGTTTCAACCATTCCATACAACTCTTAGAAGAGCTTGGATTTCCCAAAGGTGTTCTTCCCTTAAAGGATCTTGTTGAATGTGGAAGAGTTAGAGAAACTGGCTTTGTTTGGATGAAACAAAAGGCACCAAGTGAACATTATTTTGAAGGTACAAAGACTCTTGTGAGCTATGGAATTGAAGTCACTGCCTATGTGGAGAAATTCAAGATGAAGAAAATGAGTGGAATTAAGAGTAAGCAATTGTTTGTTTGGGTGCCTATAGTTGAAATGAGTATTGAGGGTTTTAATGGAAAGAAGATGTATTTTAAGACTCCTATGGGGATTGGTAAGTCTTTTCATGTTACTTCATTTATGAGTGTTGAGGAAAAGGAAAAGTATGAGAAGTTGCAATTGAAGGATAAGGAGGTTGATGAGATCAAAGAAAATTAA

Protein sequence

>MS.gene068924.t1

MASNSTMVTNERAGAEIVYGSEECFNHSIQLLEELGFPKGVLPLKDLVECGRVRETGFVWMKQKAPSEHYFEGTKTLVSYGIEVTAYVEKFKMKKMSGIKSKQLFVWVPIVEMSIEGFNGKKMYFKTPMGIGKSFHVTSFMSVEEKEKYEKLQLKDKEVDEIKEN