AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056163

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056163.t1 MTR_8g037270 95.238 294 14 0 1 294 1 294 0.0 582
MS.gene056163.t1 MTR_3g061510 45.933 209 91 5 21 209 20 226 3.82e-47 161
MS.gene056163.t1 MTR_3g061510 45.933 209 91 5 21 209 40 246 6.32e-47 161
MS.gene056163.t1 MTR_2g041090 49.669 151 67 4 114 259 33 179 4.99e-37 132
MS.gene056163.t1 MTR_2g030170 71.111 90 26 0 127 216 86 175 2.03e-36 129
MS.gene056163.t1 MTR_2g030170 73.494 83 22 0 134 216 126 208 8.52e-34 124
MS.gene056163.t1 MTR_8g019540 45.614 171 71 7 115 283 128 278 4.13e-33 124
MS.gene056163.t1 MTR_2g099490 59.223 103 39 2 115 216 134 234 6.82e-32 121
MS.gene056163.t1 MTR_2g099490 52.137 117 41 2 115 216 134 250 9.43e-31 118
MS.gene056163.t1 MTR_7g106450 51.200 125 47 5 98 219 118 231 1.72e-30 117
MS.gene056163.t1 MTR_1g056530 49.593 123 49 4 109 229 128 239 8.50e-29 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056163.t1 AT5G12840 38.430 242 122 7 27 245 25 262 1.79e-39 139
MS.gene056163.t1 AT5G12840 38.430 242 122 7 27 245 25 262 1.79e-39 139
MS.gene056163.t1 AT5G12840 47.917 144 74 1 103 245 120 263 4.33e-39 138
MS.gene056163.t1 AT5G12840 47.917 144 74 1 103 245 120 263 4.33e-39 138
MS.gene056163.t1 AT5G12840 47.917 144 74 1 103 245 120 263 4.33e-39 138
MS.gene056163.t1 AT3G20910 47.619 147 75 1 98 242 108 254 6.36e-39 139
MS.gene056163.t1 AT1G17590 46.857 175 70 9 51 216 74 234 1.48e-32 122
MS.gene056163.t1 AT1G17590 46.857 175 70 9 51 216 74 234 1.48e-32 122
MS.gene056163.t1 AT1G17590 46.857 175 70 9 51 216 74 234 1.48e-32 122
MS.gene056163.t1 AT1G17590 46.857 175 70 9 51 216 74 234 1.48e-32 122
MS.gene056163.t1 AT1G72830 56.250 112 46 3 115 223 137 248 2.21e-32 122
MS.gene056163.t1 AT1G30500 50.758 132 57 3 114 237 59 190 4.24e-31 115
MS.gene056163.t1 AT1G30500 62.245 98 30 2 147 237 89 186 6.01e-31 114
MS.gene056163.t1 AT1G72830 55.752 113 46 4 115 223 137 249 1.16e-30 118
MS.gene056163.t1 AT3G14020 59.794 97 36 2 129 224 145 239 1.57e-30 117
MS.gene056163.t1 AT3G14020 59.794 97 36 2 129 224 145 239 1.57e-30 117
MS.gene056163.t1 AT5G06510 36.453 203 85 7 30 213 13 190 5.87e-30 114
MS.gene056163.t1 AT5G06510 36.453 203 85 7 30 213 13 190 5.87e-30 114
MS.gene056163.t1 AT5G06510 36.453 203 85 7 30 213 13 190 5.87e-30 114
MS.gene056163.t1 AT5G06510 36.453 203 85 7 30 213 13 190 5.87e-30 114
MS.gene056163.t1 AT2G34720 46.154 143 70 4 102 239 45 185 1.12e-29 112
MS.gene056163.t1 AT2G34720 49.231 130 60 3 114 239 23 150 1.86e-29 110
MS.gene056163.t1 AT2G34720 49.231 130 60 3 114 239 23 150 1.86e-29 110
MS.gene056163.t1 AT3G05690 35.102 245 118 9 31 255 18 241 2.25e-29 113
MS.gene056163.t1 AT3G05690 32.959 267 138 9 9 255 33 278 2.61e-29 114
MS.gene056163.t1 AT1G54160 69.333 75 22 1 143 216 166 240 4.75e-29 113
MS.gene056163.t1 AT5G06510 55.102 98 29 3 119 213 10 95 5.58e-28 106
MS.gene056163.t1 AT5G06510 55.102 98 29 3 119 213 10 95 5.58e-28 106
MS.gene056163.t1 AT5G06510 55.102 98 29 3 119 213 56 141 2.32e-27 106
MS.gene056163.t1 AT1G72830 54.217 83 38 0 141 223 141 223 1.95e-22 95.1

Find 70 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAGTTCCGTCTGCGAATT+TGG 0.146875 8.1:+66095220 None:intergenic
ATTCTCCAATCATCAGTTTC+TGG 0.161621 8.1:+66095963 None:intergenic
CCGAATTGTGCATTTGAATA+AGG 0.229160 8.1:+66095102 None:intergenic
GGTCAAATCTTGAATGCTTA+TGG 0.231694 8.1:-66095081 MS.gene056163:CDS
TCTAATGCTGCTCTAGAAAA+AGG 0.271832 8.1:-66093513 MS.gene056163:CDS
CAGCCTTGGTGGCTTGAAAA+TGG 0.313297 8.1:-66095891 MS.gene056163:CDS
AAACAGGAGGGTGACTTCTT+TGG 0.313426 8.1:-66093342 MS.gene056163:CDS
ACAGGGCATGTTTGATGTTT+CGG 0.314790 8.1:+66095278 None:intergenic
AGGCTGATGATGAGAGTAAA+TGG 0.326121 8.1:+66095908 None:intergenic
CTGATCAGTTGAAGATGGTT+TGG 0.350260 8.1:+66095866 None:intergenic
GCATTCTAGCATGATGCATT+TGG 0.399038 8.1:+66094156 None:intergenic
TTGAGAAGGGCAAGGGGAAA+TGG 0.400323 8.1:-66093576 MS.gene056163:CDS
TGTCACACATTCAGAGCCTT+TGG 0.400488 8.1:-66095820 MS.gene056163:CDS
GAAAGTTATTAAAGTTAGAA+AGG 0.407898 8.1:-66094030 MS.gene056163:intron
TGAGCCTTGATTCTCATTGT+TGG 0.412750 8.1:+66093464 None:intergenic
TGGTAAATGCGGTTGAGGAC+AGG 0.424267 8.1:+66095260 None:intergenic
TCATCAGTTTCTGGTTTCCC+TGG 0.428859 8.1:+66095972 None:intergenic
TTCACAGGCAAATGGAAAAC+AGG 0.431715 8.1:-66093358 MS.gene056163:CDS
AAAAGCTGTGAAAGGATGCC+AGG 0.439397 8.1:-66095990 None:intergenic
CCTTATTCAAATGCACAATT+CGG 0.445518 8.1:-66095102 MS.gene056163:CDS
ACTCTCATCATCAGCCTTGG+TGG 0.446091 8.1:-66095902 MS.gene056163:CDS
TTTAGCTGATCAGTTGAAGA+TGG 0.474075 8.1:+66095861 None:intergenic
GTTCGAGCTTCTTTGTATTG+AGG 0.481503 8.1:+66093544 None:intergenic
GCAGCACATGCACAAAATGC+AGG 0.482353 8.1:-66093430 MS.gene056163:CDS
CTATGTCATTCACAGGCAAA+TGG 0.486227 8.1:-66093366 MS.gene056163:CDS
TTTACTCTCATCATCAGCCT+TGG 0.487165 8.1:-66095905 MS.gene056163:CDS
GGAAACCAGAAACTGATGAT+TGG 0.490368 8.1:-66095968 MS.gene056163:CDS
AACAATACTGGTGCAACATC+GGG 0.490937 8.1:-66093489 MS.gene056163:CDS
GTCCATGGTAAATGCGGTTG+AGG 0.491108 8.1:+66095255 None:intergenic
TCTCAAAGCATGTTGGTGAC+GGG 0.492087 8.1:+66093593 None:intergenic
TTCTCAAAGCATGTTGGTGA+CGG 0.494518 8.1:+66093592 None:intergenic
TGACGGGATTCATGAAGATA+TGG 0.495855 8.1:+66093609 None:intergenic
AATTCGCAGACGGAACTTGT+TGG 0.504090 8.1:-66095217 MS.gene056163:CDS
GAGAATAAAACATAGTGAGC+AGG 0.507446 8.1:-66095946 MS.gene056163:CDS
CTCAGGTGGGGACGTTGCCA+AGG 0.514046 8.1:-66095305 MS.gene056163:intron
TTCAACATCAGTTACCATGA+TGG 0.518987 8.1:-66093405 MS.gene056163:CDS
GTCAATGCAAAGCAGTATCA+TGG 0.520607 8.1:-66094098 MS.gene056163:CDS
TGGACCAAGGTGTTTGTCCA+TGG 0.523692 8.1:+66095240 None:intergenic
TTTGATGTTTCGGTGCTCCT+TGG 0.525070 8.1:+66095288 None:intergenic
CAACAATACTGGTGCAACAT+CGG 0.530920 8.1:-66093490 MS.gene056163:CDS
TGCTTTGCATTGACATACAC+AGG 0.531370 8.1:+66094107 None:intergenic
GGTAAATGCGGTTGAGGACA+GGG 0.539516 8.1:+66095261 None:intergenic
TTGCCCTTCTCAAAGCATGT+TGG 0.541677 8.1:+66093586 None:intergenic
CTGTAAAGTCATTTCCATCA+TGG 0.545256 8.1:+66093391 None:intergenic
TACAGCACTATGTCATTCAC+AGG 0.550828 8.1:-66093373 MS.gene056163:CDS
TGCTTATGGACAACAAGCTA+TGG 0.551820 8.1:-66095067 MS.gene056163:intron
TTTGCCGCTTAAAATGGAAG+AGG 0.565386 8.1:-66094132 MS.gene056163:CDS
ACATGCTTTGAGAAGGGCAA+GGG 0.570857 8.1:-66093583 MS.gene056163:CDS
GTCACCAACATGCTTTGAGA+AGG 0.572667 8.1:-66093590 MS.gene056163:CDS
ACGAAGACAATCGCGTGCAA+AGG 0.578373 8.1:-66094066 MS.gene056163:CDS
GGTACCAACAATGAGAATCA+AGG 0.578464 8.1:-66093468 MS.gene056163:CDS
TTTACCATGGACAAACACCT+TGG 0.581517 8.1:-66095244 MS.gene056163:CDS
CATGCTTTGAGAAGGGCAAG+GGG 0.591440 8.1:-66093582 MS.gene056163:CDS
GATGCATTTGGTATAGCTGA+GGG 0.592164 8.1:+66094168 None:intergenic
TTATCAGTTAGAGAAAGTGA+AGG 0.593911 8.1:+66095792 None:intergenic
TGATGCATTTGGTATAGCTG+AGG 0.596710 8.1:+66094167 None:intergenic
GAAAGTGAAGGAGAAACCAA+AGG 0.608239 8.1:+66095804 None:intergenic
TCACCAACATGCTTTGAGAA+GGG 0.609839 8.1:-66093589 MS.gene056163:CDS
GTCCTCAACCGCATTTACCA+TGG 0.610316 8.1:-66095257 MS.gene056163:CDS
CTTTCTCTAACTGATAACTC+AGG 0.610628 8.1:-66095786 MS.gene056163:intron
TGATGAGAGTAAATGGAAGA+TGG 0.612845 8.1:+66095915 None:intergenic
CAGCACATGCACAAAATGCA+GGG 0.616147 8.1:-66093429 MS.gene056163:CDS
AAAGCTGTGAAAGGATGCCA+GGG 0.629084 8.1:-66095989 None:intergenic
AACATGCTTTGAGAAGGGCA+AGG 0.629479 8.1:-66093584 MS.gene056163:CDS
ACAGGCAAATGGAAAACAGG+AGG 0.631076 8.1:-66093355 MS.gene056163:CDS
CAGGCAAATGGAAAACAGGA+GGG 0.651426 8.1:-66093354 MS.gene056163:CDS
AGAAGGGCAAGGGGAAATGG+CGG 0.651466 8.1:-66093573 MS.gene056163:CDS
CCTTGGTCCAAATTCGCAGA+CGG 0.654666 8.1:-66095227 MS.gene056163:CDS
CCGTCTGCGAATTTGGACCA+AGG 0.662623 8.1:+66095227 None:intergenic
GTGTTTGTCCATGGTAAATG+CGG 0.682262 8.1:+66095249 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTTTTTTACAAATATG+TGG - chr8.1:66094535-66094554 MS.gene056163:intron 10.0%
!! ATGAACAATAATAGTAAATA+AGG + chr8.1:66093746-66093765 None:intergenic 15.0%
!! TGAACAATAATAGTAAATAA+GGG + chr8.1:66093745-66093764 None:intergenic 15.0%
!! TTCATAAAAAAATGTCTTAT+TGG - chr8.1:66093954-66093973 MS.gene056163:intron 15.0%
!!! AAGACGTTTTTTATTTTTAA+GGG - chr8.1:66095001-66095020 MS.gene056163:intron 15.0%
!!! ATATTAAGTGACATAATTTT+AGG - chr8.1:66093643-66093662 MS.gene056163:intron 15.0%
!! AACAATAATAGTAAATAAGG+GGG + chr8.1:66093743-66093762 None:intergenic 20.0%
!! AACAATATTAAAAGACTGAT+AGG + chr8.1:66095027-66095046 None:intergenic 20.0%
!! ACTATTATTGTTCATAATCA+AGG - chr8.1:66093751-66093770 MS.gene056163:intron 20.0%
!! ATACTATCTTAATTGTATCT+TGG - chr8.1:66095473-66095492 MS.gene056163:intron 20.0%
!! CATTGATATGATGAAAAAAA+TGG - chr8.1:66095305-66095324 MS.gene056163:intron 20.0%
!! CTATTATTGTTCATAATCAA+GGG - chr8.1:66093752-66093771 MS.gene056163:intron 20.0%
!! CTTAAAAATAAAAAACGTCT+TGG + chr8.1:66095002-66095021 None:intergenic 20.0%
!! GAAAGTTATTAAAGTTAGAA+AGG - chr8.1:66095253-66095272 MS.gene056163:CDS 20.0%
!! GAACAATAATAGTAAATAAG+GGG + chr8.1:66093744-66093763 None:intergenic 20.0%
!! TAATGATAAAATAGTTCGTA+AGG - chr8.1:66094558-66094577 MS.gene056163:intron 20.0%
!! TTCTTAATATAAAACTCTTG+AGG + chr8.1:66095437-66095456 None:intergenic 20.0%
!!! ATATCAATGATAATGCTATA+AGG + chr8.1:66095294-66095313 None:intergenic 20.0%
!!! CAAGACGTTTTTTATTTTTA+AGG - chr8.1:66095000-66095019 MS.gene056163:intron 20.0%
!!! GATAATTTGTTTATTTCTTG+TGG - chr8.1:66094348-66094367 MS.gene056163:intron 20.0%
! AAGATTAAGTAGTTTGGAAA+AGG - chr8.1:66095409-66095428 MS.gene056163:intron 25.0%
! AAGCAATCAACACATAATAT+CGG + chr8.1:66094740-66094759 None:intergenic 25.0%
! ACAATTACTAAGTACTAACA+TGG + chr8.1:66094835-66094854 None:intergenic 25.0%
! ATAAACAAGAAAGTTTGAAG+AGG + chr8.1:66093539-66093558 None:intergenic 25.0%
! ATATTCATTTATTCCTTGGA+AGG - chr8.1:66094448-66094467 MS.gene056163:intron 25.0%
! GGTAAAAAGATTGAATCAAA+TGG - chr8.1:66095962-66095981 MS.gene056163:CDS 25.0%
! TATGATCAATTCTACACAAA+TGG + chr8.1:66095625-66095644 None:intergenic 25.0%
! TCCTAAAAGATTAAGTAGTT+TGG - chr8.1:66095403-66095422 MS.gene056163:intron 25.0%
!! ACTTACCTTCTTTTTATTCA+TGG - chr8.1:66094321-66094340 MS.gene056163:intron 25.0%
!! ATTTGTTTATTTCTTGTGGT+TGG - chr8.1:66094352-66094371 MS.gene056163:intron 25.0%
!! CAATTCAAGTAACTTTTTGA+AGG - chr8.1:66093786-66093805 MS.gene056163:intron 25.0%
!! CTTACCTTCTTTTTATTCAT+GGG - chr8.1:66094322-66094341 MS.gene056163:intron 25.0%
!! TCCAAACTACTTAATCTTTT+AGG + chr8.1:66095407-66095426 None:intergenic 25.0%
!!! ACTTAATCTTTTAGGAAAGT+TGG + chr8.1:66095399-66095418 None:intergenic 25.0%
!!! CTCTTTTTTAATCTATAGAC+TGG - chr8.1:66094297-66094316 MS.gene056163:intron 25.0%
AAAGATGAATCTTGTGCAAA+AGG + chr8.1:66094917-66094936 None:intergenic 30.0%
AAATATAGAAACCATTCCCA+AGG + chr8.1:66095524-66095543 None:intergenic 30.0%
AATATAGAAACCATTCCCAA+GGG + chr8.1:66095523-66095542 None:intergenic 30.0%
ATTGTATCTTGGATCAATCT+AGG - chr8.1:66095484-66095503 MS.gene056163:intron 30.0%
CAGCATATTCATTTATTCCT+TGG - chr8.1:66094444-66094463 MS.gene056163:intron 30.0%
CCTTATTCAAATGCACAATT+CGG - chr8.1:66094181-66094200 MS.gene056163:intron 30.0%
CTTTCCCATGAATAAAAAGA+AGG + chr8.1:66094329-66094348 None:intergenic 30.0%
TCTAGATACCAAATTGCAAA+TGG + chr8.1:66093669-66093688 None:intergenic 30.0%
TCTAGATTTGTCAAACCAAT+TGG - chr8.1:66093683-66093702 MS.gene056163:intron 30.0%
TCTCTTATTCGATGAAGATT+AGG - chr8.1:66095563-66095582 MS.gene056163:intron 30.0%
TTATCAGTTAGAGAAAGTGA+AGG + chr8.1:66093494-66093513 None:intergenic 30.0%
TTGTATCTTGGATCAATCTA+GGG - chr8.1:66095485-66095504 MS.gene056163:intron 30.0%
TTTCCATGAAAAAGTCCAAT+TGG + chr8.1:66093701-66093720 None:intergenic 30.0%
! AAAAATGTCTTATTGGTCTC+AGG - chr8.1:66093961-66093980 MS.gene056163:intron 30.0%
! AGATTCATCTTTTCTCTGTA+TGG - chr8.1:66094925-66094944 MS.gene056163:intron 30.0%
! ATTTTAGGCCATTTGCAATT+TGG - chr8.1:66093658-66093677 MS.gene056163:intron 30.0%
! TTTTCCAAGATATGTACACA+TGG - chr8.1:66094120-66094139 MS.gene056163:CDS 30.0%
!! CTTCTATTTCTGAAGTATCT+GGG + chr8.1:66094608-66094627 None:intergenic 30.0%
!! TCTTCTATTTCTGAAGTATC+TGG + chr8.1:66094609-66094628 None:intergenic 30.0%
!! TCTTTTAGGAAAGTTGGTTT+TGG + chr8.1:66095393-66095412 None:intergenic 30.0%
!!! AAACCAATTGGACTTTTTCA+TGG - chr8.1:66093695-66093714 MS.gene056163:intron 30.0%
AAAACGTCTTGGACGAAAAA+AGG + chr8.1:66094991-66095010 None:intergenic 35.0%
ATATTGTGTGCACCTATATC+TGG - chr8.1:66094387-66094406 MS.gene056163:intron 35.0%
ATCTTGTGCAAAAGGTAATG+AGG + chr8.1:66094909-66094928 None:intergenic 35.0%
ATTCTCCAATCATCAGTTTC+TGG + chr8.1:66093323-66093342 None:intergenic 35.0%
CCGAATTGTGCATTTGAATA+AGG + chr8.1:66094184-66094203 None:intergenic 35.0%
CTAGAAAAAGGCAACAATAC+TGG - chr8.1:66095782-66095801 MS.gene056163:intron 35.0%
CTGTAAAGTCATTTCCATCA+TGG + chr8.1:66095895-66095914 None:intergenic 35.0%
CTTTCTCTAACTGATAACTC+AGG - chr8.1:66093497-66093516 MS.gene056163:CDS 35.0%
GAAAAAAGGAAGAGAAAGAG+AGG + chr8.1:66094977-66094996 None:intergenic 35.0%
GAGAATAAAACATAGTGAGC+AGG - chr8.1:66093337-66093356 MS.gene056163:CDS 35.0%
GGTCAAATCTTGAATGCTTA+TGG - chr8.1:66094202-66094221 MS.gene056163:intron 35.0%
TAATATGCAATGTCCTTCCA+AGG + chr8.1:66094464-66094483 None:intergenic 35.0%
TATTGTGTGCACCTATATCT+GGG - chr8.1:66094388-66094407 MS.gene056163:intron 35.0%
TCTTGTGCAAAAGGTAATGA+GGG + chr8.1:66094908-66094927 None:intergenic 35.0%
TGATGAGAGTAAATGGAAGA+TGG + chr8.1:66093371-66093390 None:intergenic 35.0%
TTCAACATCAGTTACCATGA+TGG - chr8.1:66095878-66095897 MS.gene056163:CDS 35.0%
TTCACCATGTGTACATATCT+TGG + chr8.1:66094127-66094146 None:intergenic 35.0%
TTTAGCTGATCAGTTGAAGA+TGG + chr8.1:66093425-66093444 None:intergenic 35.0%
! CTTTTGTCATATCTGGTTAC+AGG - chr8.1:66094147-66094166 MS.gene056163:CDS 35.0%
! TGGTGAACTTTTGTCATATC+TGG - chr8.1:66094140-66094159 MS.gene056163:CDS 35.0%
!! AATAGAAGAGTATCAGTGTC+CGG - chr8.1:66094620-66094639 MS.gene056163:intron 35.0%
!! AATGCTTTTGCCGCTTAAAA+TGG - chr8.1:66095145-66095164 MS.gene056163:intron 35.0%
!! TCTAATGCTGCTCTAGAAAA+AGG - chr8.1:66095770-66095789 MS.gene056163:intron 35.0%
AACAATACTGGTGCAACATC+GGG - chr8.1:66095794-66095813 MS.gene056163:CDS 40.0%
ACAGGGCATGTTTGATGTTT+CGG + chr8.1:66094008-66094027 None:intergenic 40.0%
ACTTAATTAGCCCTTGGGAA+TGG - chr8.1:66095510-66095529 MS.gene056163:intron 40.0%
AGGGTAACTTAATTAGCCCT+TGG - chr8.1:66095504-66095523 MS.gene056163:intron 40.0%
CAACAATACTGGTGCAACAT+CGG - chr8.1:66095793-66095812 MS.gene056163:CDS 40.0%
CTATGTCATTCACAGGCAAA+TGG - chr8.1:66095917-66095936 MS.gene056163:CDS 40.0%
CTGATCAGTTGAAGATGGTT+TGG + chr8.1:66093420-66093439 None:intergenic 40.0%
GAAAGTGAAGGAGAAACCAA+AGG + chr8.1:66093482-66093501 None:intergenic 40.0%
GATGCATTTGGTATAGCTGA+GGG + chr8.1:66095118-66095137 None:intergenic 40.0%
GCATAATGCCAATAGCTGTT+AGG + chr8.1:66095087-66095106 None:intergenic 40.0%
GCATTCTAGCATGATGCATT+TGG + chr8.1:66095130-66095149 None:intergenic 40.0%
GGAAACCAGAAACTGATGAT+TGG - chr8.1:66093315-66093334 MS.gene056163:CDS 40.0%
GGCTAAGATGTGTAAGCAAA+AGG + chr8.1:66093583-66093602 None:intergenic 40.0%
GGGTAACTTAATTAGCCCTT+GGG - chr8.1:66095505-66095524 MS.gene056163:intron 40.0%
GGTACCAACAATGAGAATCA+AGG - chr8.1:66095815-66095834 MS.gene056163:CDS 40.0%
GTATCTTTAGCTGTAAGCCT+CGG - chr8.1:66094871-66094890 MS.gene056163:intron 40.0%
GTCAATGCAAAGCAGTATCA+TGG - chr8.1:66095185-66095204 MS.gene056163:intron 40.0%
GTGTTTGTCCATGGTAAATG+CGG + chr8.1:66094037-66094056 None:intergenic 40.0%
GTTCGAGCTTCTTTGTATTG+AGG + chr8.1:66095742-66095761 None:intergenic 40.0%
TACAGATAACACAAGCATGC+TGG + chr8.1:66093604-66093623 None:intergenic 40.0%
TACAGCACTATGTCATTCAC+AGG - chr8.1:66095910-66095929 MS.gene056163:CDS 40.0%
TATCGCTATCGAATATGTGC+CGG + chr8.1:66094642-66094661 None:intergenic 40.0%
TGACGGGATTCATGAAGATA+TGG + chr8.1:66095677-66095696 None:intergenic 40.0%
TGAGCCTTGATTCTCATTGT+TGG + chr8.1:66095822-66095841 None:intergenic 40.0%
TGATGCATTTGGTATAGCTG+AGG + chr8.1:66095119-66095138 None:intergenic 40.0%
TGCTTATGGACAACAAGCTA+TGG - chr8.1:66094216-66094235 MS.gene056163:intron 40.0%
TTCACAGGCAAATGGAAAAC+AGG - chr8.1:66095925-66095944 MS.gene056163:CDS 40.0%
TTTACCATGGACAAACACCT+TGG - chr8.1:66094039-66094058 MS.gene056163:CDS 40.0%
TTTACTCTCATCATCAGCCT+TGG - chr8.1:66093378-66093397 MS.gene056163:CDS 40.0%
! AATGTCTTATTGGTCTCAGG+TGG - chr8.1:66093964-66093983 MS.gene056163:intron 40.0%
! AGGCTGATGATGAGAGTAAA+TGG + chr8.1:66093378-66093397 None:intergenic 40.0%
! ATGTCTTATTGGTCTCAGGT+GGG - chr8.1:66093965-66093984 MS.gene056163:intron 40.0%
! ATTTCTTGTGGTTGGTGTCT+AGG - chr8.1:66094360-66094379 MS.gene056163:intron 40.0%
! GGTTCCTCTTCCATTTTAAG+CGG + chr8.1:66095158-66095177 None:intergenic 40.0%
! TGCTTTGCATTGACATACAC+AGG + chr8.1:66095179-66095198 None:intergenic 40.0%
! TTCTCAAAGCATGTTGGTGA+CGG + chr8.1:66095694-66095713 None:intergenic 40.0%
! TTTGCCGCTTAAAATGGAAG+AGG - chr8.1:66095151-66095170 MS.gene056163:intron 40.0%
!! TCACCAACATGCTTTGAGAA+GGG - chr8.1:66095694-66095713 MS.gene056163:intron 40.0%
AAACAGGAGGGTGACTTCTT+TGG - chr8.1:66095941-66095960 MS.gene056163:CDS 45.0%
AATTCGCAGACGGAACTTGT+TGG - chr8.1:66094066-66094085 MS.gene056163:CDS 45.0%
ACAAGTTCCGTCTGCGAATT+TGG + chr8.1:66094066-66094085 None:intergenic 45.0%
ACAGGCAAATGGAAAACAGG+AGG - chr8.1:66095928-66095947 MS.gene056163:CDS 45.0%
ACTCGAGTCCTAACAGCTAT+TGG - chr8.1:66095076-66095095 MS.gene056163:CDS 45.0%
AGCCTATCATGCCCAGATAT+AGG + chr8.1:66094402-66094421 None:intergenic 45.0%
CACCTATATCTGGGCATGAT+AGG - chr8.1:66094397-66094416 MS.gene056163:intron 45.0%
CAGCACATGCACAAAATGCA+GGG - chr8.1:66095854-66095873 MS.gene056163:CDS 45.0%
CAGGCAAATGGAAAACAGGA+GGG - chr8.1:66095929-66095948 MS.gene056163:CDS 45.0%
TGTCACACATTCAGAGCCTT+TGG - chr8.1:66093463-66093482 MS.gene056163:CDS 45.0%
TTGCCCTTCTCAAAGCATGT+TGG + chr8.1:66095700-66095719 None:intergenic 45.0%
! TGTCTTATTGGTCTCAGGTG+GGG - chr8.1:66093966-66093985 MS.gene056163:intron 45.0%
!! AACATGCTTTGAGAAGGGCA+AGG - chr8.1:66095699-66095718 MS.gene056163:intron 45.0%
!! ACATGCTTTGAGAAGGGCAA+GGG - chr8.1:66095700-66095719 MS.gene056163:intron 45.0%
!! GTCACCAACATGCTTTGAGA+AGG - chr8.1:66095693-66095712 MS.gene056163:intron 45.0%
!! TCATCAGTTTCTGGTTTCCC+TGG + chr8.1:66093314-66093333 None:intergenic 45.0%
!! TCTCAAAGCATGTTGGTGAC+GGG + chr8.1:66095693-66095712 None:intergenic 45.0%
!! TTTGATGTTTCGGTGCTCCT+TGG + chr8.1:66093998-66094017 None:intergenic 45.0%
!!! AAAGTAATTTAAATTTTTAT+CGG + chr8.1:66094703-66094722 None:intergenic 5.0%
ACGAAGACAATCGCGTGCAA+AGG - chr8.1:66095217-66095236 MS.gene056163:CDS 50.0%
ACTCTCATCATCAGCCTTGG+TGG - chr8.1:66093381-66093400 MS.gene056163:CDS 50.0%
CCTTGGTCCAAATTCGCAGA+CGG - chr8.1:66094056-66094075 MS.gene056163:CDS 50.0%
GCAGCACATGCACAAAATGC+AGG - chr8.1:66095853-66095872 MS.gene056163:CDS 50.0%
GGTAAATGCGGTTGAGGACA+GGG + chr8.1:66094025-66094044 None:intergenic 50.0%
GTCCATGGTAAATGCGGTTG+AGG + chr8.1:66094031-66094050 None:intergenic 50.0%
GTCCTCAACCGCATTTACCA+TGG - chr8.1:66094026-66094045 MS.gene056163:intron 50.0%
TGGTAAATGCGGTTGAGGAC+AGG + chr8.1:66094026-66094045 None:intergenic 50.0%
TTGAGAAGGGCAAGGGGAAA+TGG - chr8.1:66095707-66095726 MS.gene056163:intron 50.0%
! CAGCCTTGGTGGCTTGAAAA+TGG - chr8.1:66093392-66093411 MS.gene056163:CDS 50.0%
! TGGACCAAGGTGTTTGTCCA+TGG + chr8.1:66094046-66094065 None:intergenic 50.0%
!! CATGCTTTGAGAAGGGCAAG+GGG - chr8.1:66095701-66095720 MS.gene056163:intron 50.0%
!! GAGCCATTTTCAAGCCACCA+AGG + chr8.1:66093398-66093417 None:intergenic 50.0%
AGAAGGGCAAGGGGAAATGG+CGG - chr8.1:66095710-66095729 MS.gene056163:intron 55.0%
CCGTCTGCGAATTTGGACCA+AGG + chr8.1:66094059-66094078 None:intergenic 55.0%
TGAGGGAAACAAGAGAGCCG+AGG + chr8.1:66094891-66094910 None:intergenic 55.0%
CTCAGGTGGGGACGTTGCCA+AGG - chr8.1:66093978-66093997 MS.gene056163:intron 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 66093308 66095997 66093308 ID=MS.gene056163
chr8.1 mRNA 66093308 66095997 66093308 ID=MS.gene056163.t1;Parent=MS.gene056163
chr8.1 exon 66095787 66095997 66095787 ID=MS.gene056163.t1.exon1;Parent=MS.gene056163.t1
chr8.1 CDS 66095787 66095997 66095787 ID=cds.MS.gene056163.t1;Parent=MS.gene056163.t1
chr8.1 exon 66095207 66095322 66095207 ID=MS.gene056163.t1.exon2;Parent=MS.gene056163.t1
chr8.1 CDS 66095207 66095322 66095207 ID=cds.MS.gene056163.t1;Parent=MS.gene056163.t1
chr8.1 exon 66095068 66095136 66095068 ID=MS.gene056163.t1.exon3;Parent=MS.gene056163.t1
chr8.1 CDS 66095068 66095136 66095068 ID=cds.MS.gene056163.t1;Parent=MS.gene056163.t1
chr8.1 exon 66094031 66094195 66094031 ID=MS.gene056163.t1.exon4;Parent=MS.gene056163.t1
chr8.1 CDS 66094031 66094195 66094031 ID=cds.MS.gene056163.t1;Parent=MS.gene056163.t1
chr8.1 exon 66093308 66093631 66093308 ID=MS.gene056163.t1.exon5;Parent=MS.gene056163.t1
chr8.1 CDS 66093308 66093631 66093308 ID=cds.MS.gene056163.t1;Parent=MS.gene056163.t1
Gene Sequence

>MS.gene056163

ATGCCAGGGAAACCAGAAACTGATGATTGGAGAATAAAACATAGTGAGCAGGTTCAGTTGCCATCTTCCATTTACTCTCATCATCAGCCTTGGTGGCTTGAAAATGGCTCCAAACCATCTTCAACTGATCAGCTAAATAGTTCAATCATGAATGATGTCACACATTCAGAGCCTTTGGTTTCTCCTTCACTTTCTCTAACTGATAACTCAGGTGTCTGTATTTTTATGCCTCTTCAAACTTTCTTGTTTATAGTGTATTTTTGTTTTTACTTCCTTTTGCTTACACATCTTAGCCAGCATGCTTGTGTTATCTGTAGTAATTTATCTGTTTTGATATATTAAGTGACATAATTTTAGGCCATTTGCAATTTGGTATCTAGATTTGTCAAACCAATTGGACTTTTTCATGGAAACGAACGTATAAGCATTTTACCCCCTTATTTACTATTATTGTTCATAATCAAGGGAAACTTATAGACAATTCAAGTAACTTTTTGAAGGAAATTTCACTCAATAATATTCTAGTTGTGTTCAAAGTGAGAGAATTGTGTCTTCAATGATGAATTTTGAATGATAGATTGAGAATTCTTAAGCACAAATTAAATGTGTCTCTTTACATGTATTAGCATTGTCTATGCACATAATATTCATAAAAAAATGTCTTATTGGTCTCAGGTGGGGACGTTGCCAAGGAGCACCGAAACATCAAACATGCCCTGTCCTCAACCGCATTTACCATGGACAAACACCTTGGTCCAAATTCGCAGACGGAACTTGTTGGTCATTCAATTGTAAGATCTTTATCATTACATTTTTCCAAGATATGTACACATGGTGAACTTTTGTCATATCTGGTTACAGGTTTTAACATCTCCTTATTCAAATGCACAATTCGGTCAAATCTTGAATGCTTATGGACAACAAGCTATGGTATGCTATTAAATATTGCTACGTCGATGTGATTGAAAATCAAACTTACTTGATCTAGTCTCTTTTTTAATCTATAGACTGGAACTTACCTTCTTTTTATTCATGGGAAAGATAATTTGTTTATTTCTTGTGGTTGGTGTCTAGGATAGATATTGTGTGCACCTATATCTGGGCATGATAGGCTTATTCGCAACATAAAATACAATCAGCATATTCATTTATTCCTTGGAAGGACATTGCATATTAATATTTATAATTATTACAAATACTATCAACAAATATGCTTGTTTGATTTTTTATTTTTTTTTACAAATATGTGGTAATGATAAAATAGTTCGTAAGGAGAATATGCGTGTAATCTATGAAGCCCAGATACTTCAGAAATAGAAGAGTATCAGTGTCCGGCACATATTCGATAGCGATACACATATGATACTATCTGATACGTACTAAAAAAAATATCCGATAAAAATTTAAATTACTTTTAAAGATACTTTCACCGATATTATGTGTTGATTGCTTGCATGTTCTTAATTGTGTGTAGATTAATCTCAATAGTAATACTTGTTACTCATTCAGTTTGAAGTAATTTGACCATGTTAGTACTTAGTAATTGTAACAACAAGCTTGAATGTATCTTTAGCTGTAAGCCTCGGCTCTCTTGTTTCCCTCATTACCTTTTGCACAAGATTCATCTTTTCTCTGTATGGACTTTTGCATCTAAATTTAAATTAAACCTCTCTTTCTCTTCCTTTTTTCGTCCAAGACGTTTTTTATTTTTAAGGGCCTATCAGTCTTTTAATATTGTTAATAGTTAATACTGCTCTGTTGCTAATTTACTCGAGTCCTAACAGCTATTGGCATTATGCTAGATGAACCCTCAGCTATACCAAATGCATCATGCTAGAATGCTTTTGCCGCTTAAAATGGAAGAGGAACCTGTGTATGTCAATGCAAAGCAGTATCATGGTATTTTGAGACGAAGACAATCGCGTGCAAAGGCTGAGCTTGAAAAGAAAGTTATTAAAGTTAGAAAGGTATGACTGATATCTACCTTATAGCATTATCATTGATATGATGAAAAAAATGGATGTAAAATTAAAATTCATTGCGTTTTATACTTGACTATCATATTTGTGTATCTTTTGATATCCAAAACCAACTTTCCTAAAAGATTAAGTAGTTTGGAAAAGGCACCTCAAGAGTTTTATATTAAGAAATATGTAAAATATCTTATACTATCTTAATTGTATCTTGGATCAATCTAGGGTAACTTAATTAGCCCTTGGGAATGGTTTCTATATTTTATCATTTATCATTTTTTTTCTCTTATTCGATGAAGATTAGGACTCTAGTACTAGTATAAATATGTATTAATGCTTTTCCATTTGTGTAGAATTGATCATAGTCACAGTTGTATCATTCATTTCTAACAGCCATATCTTCATGAATCCCGTCACCAACATGCTTTGAGAAGGGCAAGGGGAAATGGCGGTCGTTTCCTCAATACAAAGAAGCTCGAACACAACGATTCTAATGCTGCTCTAGAAAAAGGCAACAATACTGGTGCAACATCGGGTACCAACAATGAGAATCAAGGCTCATCTAATGTGTCGCAGCACATGCACAAAATGCAGGGTTTCAACATCAGTTACCATGATGGAAATGACTTTACAGCACTATGTCATTCACAGGCAAATGGAAAACAGGAGGGTGACTTCTTTGGTAAAAAGATTGAATCAAATGGTGCTATTAAATGA
Protein sequence

>MS.gene056163.t1

MPGKPETDDWRIKHSEQVQLPSSIYSHHQPWWLENGSKPSSTDQLNSSIMNDVTHSEPLVSPSLSLTDNSGGDVAKEHRNIKHALSSTAFTMDKHLGPNSQTELVGHSIVLTSPYSNAQFGQILNAYGQQAMMNPQLYQMHHARMLLPLKMEEEPVYVNAKQYHGILRRRQSRAKAELEKKVIKVRKPYLHESRHQHALRRARGNGGRFLNTKKLEHNDSNAALEKGNNTGATSGTNNENQGSSNVSQHMHKMQGFNISYHDGNDFTALCHSQANGKQEGDFFGKKIESNGAIK