AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23407

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23407.t1 MTR_8g037270 95.238 294 14 0 1 294 1 294 0.0 582
MS.gene23407.t1 MTR_3g061510 45.933 209 91 5 21 209 20 226 3.82e-47 161
MS.gene23407.t1 MTR_3g061510 45.933 209 91 5 21 209 40 246 6.32e-47 161
MS.gene23407.t1 MTR_2g041090 49.669 151 67 4 114 259 33 179 4.99e-37 132
MS.gene23407.t1 MTR_2g030170 71.111 90 26 0 127 216 86 175 2.03e-36 129
MS.gene23407.t1 MTR_2g030170 73.494 83 22 0 134 216 126 208 8.52e-34 124
MS.gene23407.t1 MTR_8g019540 45.614 171 71 7 115 283 128 278 4.13e-33 124
MS.gene23407.t1 MTR_2g099490 59.223 103 39 2 115 216 134 234 6.82e-32 121
MS.gene23407.t1 MTR_2g099490 52.137 117 41 2 115 216 134 250 9.43e-31 118
MS.gene23407.t1 MTR_7g106450 51.200 125 47 5 98 219 118 231 1.72e-30 117
MS.gene23407.t1 MTR_1g056530 49.593 123 49 4 109 229 128 239 8.50e-29 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23407.t1 AT5G12840 38.430 242 122 7 27 245 25 262 1.79e-39 139
MS.gene23407.t1 AT5G12840 38.430 242 122 7 27 245 25 262 1.79e-39 139
MS.gene23407.t1 AT5G12840 47.917 144 74 1 103 245 120 263 4.33e-39 138
MS.gene23407.t1 AT5G12840 47.917 144 74 1 103 245 120 263 4.33e-39 138
MS.gene23407.t1 AT5G12840 47.917 144 74 1 103 245 120 263 4.33e-39 138
MS.gene23407.t1 AT3G20910 47.619 147 75 1 98 242 108 254 6.36e-39 139
MS.gene23407.t1 AT1G17590 46.857 175 70 9 51 216 74 234 1.48e-32 122
MS.gene23407.t1 AT1G17590 46.857 175 70 9 51 216 74 234 1.48e-32 122
MS.gene23407.t1 AT1G17590 46.857 175 70 9 51 216 74 234 1.48e-32 122
MS.gene23407.t1 AT1G17590 46.857 175 70 9 51 216 74 234 1.48e-32 122
MS.gene23407.t1 AT1G72830 56.250 112 46 3 115 223 137 248 2.21e-32 122
MS.gene23407.t1 AT1G30500 50.758 132 57 3 114 237 59 190 4.24e-31 115
MS.gene23407.t1 AT1G30500 62.245 98 30 2 147 237 89 186 6.01e-31 114
MS.gene23407.t1 AT1G72830 55.752 113 46 4 115 223 137 249 1.16e-30 118
MS.gene23407.t1 AT3G14020 59.794 97 36 2 129 224 145 239 1.57e-30 117
MS.gene23407.t1 AT3G14020 59.794 97 36 2 129 224 145 239 1.57e-30 117
MS.gene23407.t1 AT5G06510 36.453 203 85 7 30 213 13 190 5.87e-30 114
MS.gene23407.t1 AT5G06510 36.453 203 85 7 30 213 13 190 5.87e-30 114
MS.gene23407.t1 AT5G06510 36.453 203 85 7 30 213 13 190 5.87e-30 114
MS.gene23407.t1 AT5G06510 36.453 203 85 7 30 213 13 190 5.87e-30 114
MS.gene23407.t1 AT2G34720 46.154 143 70 4 102 239 45 185 1.12e-29 112
MS.gene23407.t1 AT2G34720 49.231 130 60 3 114 239 23 150 1.86e-29 110
MS.gene23407.t1 AT2G34720 49.231 130 60 3 114 239 23 150 1.86e-29 110
MS.gene23407.t1 AT3G05690 35.102 245 118 9 31 255 18 241 2.25e-29 113
MS.gene23407.t1 AT3G05690 32.959 267 138 9 9 255 33 278 2.61e-29 114
MS.gene23407.t1 AT1G54160 69.333 75 22 1 143 216 166 240 4.75e-29 113
MS.gene23407.t1 AT5G06510 55.102 98 29 3 119 213 10 95 5.58e-28 106
MS.gene23407.t1 AT5G06510 55.102 98 29 3 119 213 10 95 5.58e-28 106
MS.gene23407.t1 AT5G06510 55.102 98 29 3 119 213 56 141 2.32e-27 106
MS.gene23407.t1 AT1G72830 54.217 83 38 0 141 223 141 223 1.95e-22 95.1

Find 70 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAGTTCCGTCTGCGAATT+TGG 0.146875 8.2:+62169197 None:intergenic
ATTCTCCAATCATCAGTTTC+TGG 0.161621 8.2:+62169940 None:intergenic
CCGAATTGTGCATTTGAATA+AGG 0.229160 8.2:+62169079 None:intergenic
GGTCAAATCTTGAATGCTTA+TGG 0.231694 8.2:-62169058 MS.gene23407:CDS
TCTAATGCTGCTCTAGAAAA+AGG 0.271832 8.2:-62167490 MS.gene23407:CDS
CAGCCTTGGTGGCTTGAAAA+TGG 0.313297 8.2:-62169868 MS.gene23407:CDS
AAACAGGAGGGTGACTTCTT+TGG 0.313426 8.2:-62167319 MS.gene23407:CDS
ACAGGGCATGTTTGATGTTT+CGG 0.314790 8.2:+62169255 None:intergenic
AGGCTGATGATGAGAGTAAA+TGG 0.326121 8.2:+62169885 None:intergenic
CTGATCAGTTGAAGATGGTT+TGG 0.350260 8.2:+62169843 None:intergenic
GCATTCTAGCATGATGCATT+TGG 0.399038 8.2:+62168133 None:intergenic
TTGAGAAGGGCAAGGGGAAA+TGG 0.400323 8.2:-62167553 MS.gene23407:CDS
TGTCACACATTCAGAGCCTT+TGG 0.400488 8.2:-62169797 MS.gene23407:CDS
GAAAGTTATTAAAGTTAGAA+AGG 0.407898 8.2:-62168007 MS.gene23407:intron
TGAGCCTTGATTCTCATTGT+TGG 0.412750 8.2:+62167441 None:intergenic
TGGTAAATGCGGTTGAGGAC+AGG 0.424267 8.2:+62169237 None:intergenic
TCATCAGTTTCTGGTTTCCC+TGG 0.428859 8.2:+62169949 None:intergenic
TTCACAGGCAAATGGAAAAC+AGG 0.431715 8.2:-62167335 MS.gene23407:CDS
AAAAGCTGTGAAAGGATGCC+AGG 0.439397 8.2:-62169967 None:intergenic
CCTTATTCAAATGCACAATT+CGG 0.445518 8.2:-62169079 MS.gene23407:CDS
ACTCTCATCATCAGCCTTGG+TGG 0.446091 8.2:-62169879 MS.gene23407:CDS
TTTAGCTGATCAGTTGAAGA+TGG 0.474075 8.2:+62169838 None:intergenic
GTTCGAGCTTCTTTGTATTG+AGG 0.481503 8.2:+62167521 None:intergenic
GCAGCACATGCACAAAATGC+AGG 0.482353 8.2:-62167407 MS.gene23407:CDS
CTATGTCATTCACAGGCAAA+TGG 0.486227 8.2:-62167343 MS.gene23407:CDS
TTTACTCTCATCATCAGCCT+TGG 0.487165 8.2:-62169882 MS.gene23407:CDS
GGAAACCAGAAACTGATGAT+TGG 0.490368 8.2:-62169945 MS.gene23407:CDS
AACAATACTGGTGCAACATC+GGG 0.490937 8.2:-62167466 MS.gene23407:CDS
GTCCATGGTAAATGCGGTTG+AGG 0.491108 8.2:+62169232 None:intergenic
TCTCAAAGCATGTTGGTGAC+GGG 0.492087 8.2:+62167570 None:intergenic
TTCTCAAAGCATGTTGGTGA+CGG 0.494518 8.2:+62167569 None:intergenic
TGACGGGATTCATGAAGATA+TGG 0.495855 8.2:+62167586 None:intergenic
AATTCGCAGACGGAACTTGT+TGG 0.504090 8.2:-62169194 MS.gene23407:CDS
GAGAATAAAACATAGTGAGC+AGG 0.507446 8.2:-62169923 MS.gene23407:CDS
CTCAGGTGGGGACGTTGCCA+AGG 0.514046 8.2:-62169282 MS.gene23407:intron
TTCAACATCAGTTACCATGA+TGG 0.518987 8.2:-62167382 MS.gene23407:CDS
GTCAATGCAAAGCAGTATCA+TGG 0.520607 8.2:-62168075 MS.gene23407:CDS
TGGACCAAGGTGTTTGTCCA+TGG 0.523692 8.2:+62169217 None:intergenic
TTTGATGTTTCGGTGCTCCT+TGG 0.525070 8.2:+62169265 None:intergenic
CAACAATACTGGTGCAACAT+CGG 0.530920 8.2:-62167467 MS.gene23407:CDS
TGCTTTGCATTGACATACAC+AGG 0.531370 8.2:+62168084 None:intergenic
GGTAAATGCGGTTGAGGACA+GGG 0.539516 8.2:+62169238 None:intergenic
TTGCCCTTCTCAAAGCATGT+TGG 0.541677 8.2:+62167563 None:intergenic
CTGTAAAGTCATTTCCATCA+TGG 0.545256 8.2:+62167368 None:intergenic
TACAGCACTATGTCATTCAC+AGG 0.550828 8.2:-62167350 MS.gene23407:CDS
TGCTTATGGACAACAAGCTA+TGG 0.551820 8.2:-62169044 MS.gene23407:intron
TTTGCCGCTTAAAATGGAAG+AGG 0.565386 8.2:-62168109 MS.gene23407:CDS
ACATGCTTTGAGAAGGGCAA+GGG 0.570857 8.2:-62167560 MS.gene23407:CDS
GTCACCAACATGCTTTGAGA+AGG 0.572667 8.2:-62167567 MS.gene23407:CDS
ACGAAGACAATCGCGTGCAA+AGG 0.578373 8.2:-62168043 MS.gene23407:CDS
GGTACCAACAATGAGAATCA+AGG 0.578464 8.2:-62167445 MS.gene23407:CDS
TTTACCATGGACAAACACCT+TGG 0.581517 8.2:-62169221 MS.gene23407:CDS
CATGCTTTGAGAAGGGCAAG+GGG 0.591440 8.2:-62167559 MS.gene23407:CDS
GATGCATTTGGTATAGCTGA+GGG 0.592164 8.2:+62168145 None:intergenic
TTATCAGTTAGAGAAAGTGA+AGG 0.593911 8.2:+62169769 None:intergenic
TGATGCATTTGGTATAGCTG+AGG 0.596710 8.2:+62168144 None:intergenic
GAAAGTGAAGGAGAAACCAA+AGG 0.608239 8.2:+62169781 None:intergenic
TCACCAACATGCTTTGAGAA+GGG 0.609839 8.2:-62167566 MS.gene23407:CDS
GTCCTCAACCGCATTTACCA+TGG 0.610316 8.2:-62169234 MS.gene23407:CDS
CTTTCTCTAACTGATAACTC+AGG 0.610628 8.2:-62169763 MS.gene23407:intron
TGATGAGAGTAAATGGAAGA+TGG 0.612845 8.2:+62169892 None:intergenic
CAGCACATGCACAAAATGCA+GGG 0.616147 8.2:-62167406 MS.gene23407:CDS
AAAGCTGTGAAAGGATGCCA+GGG 0.629084 8.2:-62169966 None:intergenic
AACATGCTTTGAGAAGGGCA+AGG 0.629479 8.2:-62167561 MS.gene23407:CDS
ACAGGCAAATGGAAAACAGG+AGG 0.631076 8.2:-62167332 MS.gene23407:CDS
CAGGCAAATGGAAAACAGGA+GGG 0.651426 8.2:-62167331 MS.gene23407:CDS
AGAAGGGCAAGGGGAAATGG+CGG 0.651466 8.2:-62167550 MS.gene23407:CDS
CCTTGGTCCAAATTCGCAGA+CGG 0.654666 8.2:-62169204 MS.gene23407:CDS
CCGTCTGCGAATTTGGACCA+AGG 0.662623 8.2:+62169204 None:intergenic
GTGTTTGTCCATGGTAAATG+CGG 0.682262 8.2:+62169226 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTTTTTTACAAATATG+TGG - chr8.2:62168512-62168531 MS.gene23407:intron 10.0%
!! ATGAACAATAATAGTAAATA+AGG + chr8.2:62167723-62167742 None:intergenic 15.0%
!! TGAACAATAATAGTAAATAA+GGG + chr8.2:62167722-62167741 None:intergenic 15.0%
!! TTCATAAAAAAATGTCTTAT+TGG - chr8.2:62167931-62167950 MS.gene23407:intron 15.0%
!!! AAGACGTTTTTTATTTTTAA+GGG - chr8.2:62168978-62168997 MS.gene23407:intron 15.0%
!!! ATATTAAGTGACATAATTTT+AGG - chr8.2:62167620-62167639 MS.gene23407:intron 15.0%
!! AACAATAATAGTAAATAAGG+GGG + chr8.2:62167720-62167739 None:intergenic 20.0%
!! AACAATATTAAAAGACTGAT+AGG + chr8.2:62169004-62169023 None:intergenic 20.0%
!! ACTATTATTGTTCATAATCA+AGG - chr8.2:62167728-62167747 MS.gene23407:intron 20.0%
!! ATACTATCTTAATTGTATCT+TGG - chr8.2:62169450-62169469 MS.gene23407:intron 20.0%
!! ATATTCATTTATTCCTTGAA+AGG - chr8.2:62168425-62168444 MS.gene23407:intron 20.0%
!! CATTGATATGATGAAAAAAA+TGG - chr8.2:62169282-62169301 MS.gene23407:intron 20.0%
!! CTATTATTGTTCATAATCAA+GGG - chr8.2:62167729-62167748 MS.gene23407:intron 20.0%
!! CTTAAAAATAAAAAACGTCT+TGG + chr8.2:62168979-62168998 None:intergenic 20.0%
!! GAAAGTTATTAAAGTTAGAA+AGG - chr8.2:62169230-62169249 MS.gene23407:CDS 20.0%
!! GAACAATAATAGTAAATAAG+GGG + chr8.2:62167721-62167740 None:intergenic 20.0%
!! TAATGATAAAATAGTTCGTA+AGG - chr8.2:62168535-62168554 MS.gene23407:intron 20.0%
!! TTCTTAATATAAAACTCTTG+AGG + chr8.2:62169414-62169433 None:intergenic 20.0%
!!! ATATCAATGATAATGCTATA+AGG + chr8.2:62169271-62169290 None:intergenic 20.0%
!!! CAAGACGTTTTTTATTTTTA+AGG - chr8.2:62168977-62168996 MS.gene23407:intron 20.0%
!!! GATAATTTGTTTATTTCTTG+TGG - chr8.2:62168325-62168344 MS.gene23407:intron 20.0%
! AAGATTAAGTAGTTTGGAAA+AGG - chr8.2:62169386-62169405 MS.gene23407:intron 25.0%
! AAGCAATCAACACATAATAT+CGG + chr8.2:62168717-62168736 None:intergenic 25.0%
! ACAATTACTAAGTACTAACA+TGG + chr8.2:62168812-62168831 None:intergenic 25.0%
! ATAAACAAGAAAGTTTGAAG+AGG + chr8.2:62167516-62167535 None:intergenic 25.0%
! GGTAAAAAGATTGAATCAAA+TGG - chr8.2:62169939-62169958 MS.gene23407:CDS 25.0%
! TATGATCAATTCTACACAAA+TGG + chr8.2:62169602-62169621 None:intergenic 25.0%
! TCCTAAAAGATTAAGTAGTT+TGG - chr8.2:62169380-62169399 MS.gene23407:intron 25.0%
!! ACTTACCTTCTTTTTATTCA+TGG - chr8.2:62168298-62168317 MS.gene23407:intron 25.0%
!! ATTTGTTTATTTCTTGTGGT+TGG - chr8.2:62168329-62168348 MS.gene23407:intron 25.0%
!! CAATTCAAGTAACTTTTTGA+AGG - chr8.2:62167763-62167782 MS.gene23407:intron 25.0%
!! CTTACCTTCTTTTTATTCAT+GGG - chr8.2:62168299-62168318 MS.gene23407:intron 25.0%
!! TCCAAACTACTTAATCTTTT+AGG + chr8.2:62169384-62169403 None:intergenic 25.0%
!!! ACTTAATCTTTTAGGAAAGT+TGG + chr8.2:62169376-62169395 None:intergenic 25.0%
!!! CTCTTTTTTAATCTATAGAC+TGG - chr8.2:62168274-62168293 MS.gene23407:intron 25.0%
AAAGATGAATCTTGTGCAAA+AGG + chr8.2:62168894-62168913 None:intergenic 30.0%
AAATATAGAAACCATTCCCA+AGG + chr8.2:62169501-62169520 None:intergenic 30.0%
AATATAGAAACCATTCCCAA+GGG + chr8.2:62169500-62169519 None:intergenic 30.0%
ATTGTATCTTGGATCAATCT+AGG - chr8.2:62169461-62169480 MS.gene23407:intron 30.0%
CCTTATTCAAATGCACAATT+CGG - chr8.2:62168158-62168177 MS.gene23407:intron 30.0%
CTTTCCCATGAATAAAAAGA+AGG + chr8.2:62168306-62168325 None:intergenic 30.0%
TAATATGCAATGTCCTTTCA+AGG + chr8.2:62168441-62168460 None:intergenic 30.0%
TCTAGATACCAAATTGCAAA+TGG + chr8.2:62167646-62167665 None:intergenic 30.0%
TCTAGATTTGTCAAACCAAT+TGG - chr8.2:62167660-62167679 MS.gene23407:intron 30.0%
TCTCTTATTCGATGAAGATT+AGG - chr8.2:62169540-62169559 MS.gene23407:intron 30.0%
TTATCAGTTAGAGAAAGTGA+AGG + chr8.2:62167471-62167490 None:intergenic 30.0%
TTGTATCTTGGATCAATCTA+GGG - chr8.2:62169462-62169481 MS.gene23407:intron 30.0%
TTTCCATGAAAAAGTCCAAT+TGG + chr8.2:62167678-62167697 None:intergenic 30.0%
! AAAAATGTCTTATTGGTCTC+AGG - chr8.2:62167938-62167957 MS.gene23407:intron 30.0%
! AGATTCATCTTTTCTCTGTA+TGG - chr8.2:62168902-62168921 MS.gene23407:intron 30.0%
! ATTTTAGGCCATTTGCAATT+TGG - chr8.2:62167635-62167654 MS.gene23407:intron 30.0%
! TTTTCCAAGATATGTACACA+TGG - chr8.2:62168097-62168116 MS.gene23407:CDS 30.0%
!! CTTCTATTTCTGAAGTATCT+GGG + chr8.2:62168585-62168604 None:intergenic 30.0%
!! TCTTCTATTTCTGAAGTATC+TGG + chr8.2:62168586-62168605 None:intergenic 30.0%
!! TCTTTTAGGAAAGTTGGTTT+TGG + chr8.2:62169370-62169389 None:intergenic 30.0%
!!! AAACCAATTGGACTTTTTCA+TGG - chr8.2:62167672-62167691 MS.gene23407:intron 30.0%
AAAACGTCTTGGACGAAAAA+AGG + chr8.2:62168968-62168987 None:intergenic 35.0%
ATATTGTGTGCACCTATATC+TGG - chr8.2:62168364-62168383 MS.gene23407:intron 35.0%
ATCTTGTGCAAAAGGTAATG+AGG + chr8.2:62168886-62168905 None:intergenic 35.0%
ATTCTCCAATCATCAGTTTC+TGG + chr8.2:62167300-62167319 None:intergenic 35.0%
CCGAATTGTGCATTTGAATA+AGG + chr8.2:62168161-62168180 None:intergenic 35.0%
CTAGAAAAAGGCAACAATAC+TGG - chr8.2:62169759-62169778 MS.gene23407:intron 35.0%
CTGTAAAGTCATTTCCATCA+TGG + chr8.2:62169872-62169891 None:intergenic 35.0%
CTTTCTCTAACTGATAACTC+AGG - chr8.2:62167474-62167493 MS.gene23407:CDS 35.0%
GAAAAAAGGAAGAGAAAGAG+AGG + chr8.2:62168954-62168973 None:intergenic 35.0%
GAGAATAAAACATAGTGAGC+AGG - chr8.2:62167314-62167333 MS.gene23407:CDS 35.0%
GGTCAAATCTTGAATGCTTA+TGG - chr8.2:62168179-62168198 MS.gene23407:intron 35.0%
TATTGTGTGCACCTATATCT+GGG - chr8.2:62168365-62168384 MS.gene23407:intron 35.0%
TCTTGTGCAAAAGGTAATGA+GGG + chr8.2:62168885-62168904 None:intergenic 35.0%
TGATGAGAGTAAATGGAAGA+TGG + chr8.2:62167348-62167367 None:intergenic 35.0%
TTCAACATCAGTTACCATGA+TGG - chr8.2:62169855-62169874 MS.gene23407:CDS 35.0%
TTCACCATGTGTACATATCT+TGG + chr8.2:62168104-62168123 None:intergenic 35.0%
TTTAGCTGATCAGTTGAAGA+TGG + chr8.2:62167402-62167421 None:intergenic 35.0%
! CTTTTGTCATATCTGGTTAC+AGG - chr8.2:62168124-62168143 MS.gene23407:CDS 35.0%
! TGGTGAACTTTTGTCATATC+TGG - chr8.2:62168117-62168136 MS.gene23407:CDS 35.0%
!! AATAGAAGAGTATCAGTGTC+CGG - chr8.2:62168597-62168616 MS.gene23407:intron 35.0%
!! AATGCTTTTGCCGCTTAAAA+TGG - chr8.2:62169122-62169141 MS.gene23407:intron 35.0%
!! TCTAATGCTGCTCTAGAAAA+AGG - chr8.2:62169747-62169766 MS.gene23407:intron 35.0%
AACAATACTGGTGCAACATC+GGG - chr8.2:62169771-62169790 MS.gene23407:CDS 40.0%
ACAGGGCATGTTTGATGTTT+CGG + chr8.2:62167985-62168004 None:intergenic 40.0%
ACTCAAGTCCTAACAGCTAT+TGG - chr8.2:62169053-62169072 MS.gene23407:CDS 40.0%
ACTTAATTAGCCCTTGGGAA+TGG - chr8.2:62169487-62169506 MS.gene23407:intron 40.0%
AGGGTAACTTAATTAGCCCT+TGG - chr8.2:62169481-62169500 MS.gene23407:intron 40.0%
CAACAATACTGGTGCAACAT+CGG - chr8.2:62169770-62169789 MS.gene23407:CDS 40.0%
CTATGTCATTCACAGGCAAA+TGG - chr8.2:62169894-62169913 MS.gene23407:CDS 40.0%
CTGATCAGTTGAAGATGGTT+TGG + chr8.2:62167397-62167416 None:intergenic 40.0%
GAAAGTGAAGGAGAAACCAA+AGG + chr8.2:62167459-62167478 None:intergenic 40.0%
GATGCATTTGGTATAGCTGA+GGG + chr8.2:62169095-62169114 None:intergenic 40.0%
GCATAATGCCAATAGCTGTT+AGG + chr8.2:62169064-62169083 None:intergenic 40.0%
GCATTCTAGCATGATGCATT+TGG + chr8.2:62169107-62169126 None:intergenic 40.0%
GGAAACCAGAAACTGATGAT+TGG - chr8.2:62167292-62167311 MS.gene23407:CDS 40.0%
GGCTAAGATGTGTAAGCAAA+AGG + chr8.2:62167560-62167579 None:intergenic 40.0%
GGGTAACTTAATTAGCCCTT+GGG - chr8.2:62169482-62169501 MS.gene23407:intron 40.0%
GGTACCAACAATGAGAATCA+AGG - chr8.2:62169792-62169811 MS.gene23407:CDS 40.0%
GTATCTTTAGCTGTAAGCCT+CGG - chr8.2:62168848-62168867 MS.gene23407:intron 40.0%
GTCAATGCAAAGCAGTATCA+TGG - chr8.2:62169162-62169181 MS.gene23407:intron 40.0%
GTGTTTGTCCATGGTAAATG+CGG + chr8.2:62168014-62168033 None:intergenic 40.0%
GTTCGAGCTTCTTTGTATTG+AGG + chr8.2:62169719-62169738 None:intergenic 40.0%
TACAGATAACACAAGCATGC+TGG + chr8.2:62167581-62167600 None:intergenic 40.0%
TACAGCACTATGTCATTCAC+AGG - chr8.2:62169887-62169906 MS.gene23407:CDS 40.0%
TATCGCTATCGAATATGTGC+CGG + chr8.2:62168619-62168638 None:intergenic 40.0%
TGACGGGATTCATGAAGATA+TGG + chr8.2:62169654-62169673 None:intergenic 40.0%
TGAGCCTTGATTCTCATTGT+TGG + chr8.2:62169799-62169818 None:intergenic 40.0%
TGATGCATTTGGTATAGCTG+AGG + chr8.2:62169096-62169115 None:intergenic 40.0%
TGCTTATGGACAACAAGCTA+TGG - chr8.2:62168193-62168212 MS.gene23407:intron 40.0%
TTCACAGGCAAATGGAAAAC+AGG - chr8.2:62169902-62169921 MS.gene23407:CDS 40.0%
TTTACCATGGACAAACACCT+TGG - chr8.2:62168016-62168035 MS.gene23407:CDS 40.0%
TTTACTCTCATCATCAGCCT+TGG - chr8.2:62167355-62167374 MS.gene23407:CDS 40.0%
! AATGTCTTATTGGTCTCAGG+TGG - chr8.2:62167941-62167960 MS.gene23407:intron 40.0%
! AGGCTGATGATGAGAGTAAA+TGG + chr8.2:62167355-62167374 None:intergenic 40.0%
! ATGTCTTATTGGTCTCAGGT+GGG - chr8.2:62167942-62167961 MS.gene23407:intron 40.0%
! ATTTCTTGTGGTTGGTGTCT+AGG - chr8.2:62168337-62168356 MS.gene23407:intron 40.0%
! GGTTCCTCTTCCATTTTAAG+CGG + chr8.2:62169135-62169154 None:intergenic 40.0%
! TGCTTTGCATTGACATACAC+AGG + chr8.2:62169156-62169175 None:intergenic 40.0%
! TTCTCAAAGCATGTTGGTGA+CGG + chr8.2:62169671-62169690 None:intergenic 40.0%
! TTTGCCGCTTAAAATGGAAG+AGG - chr8.2:62169128-62169147 MS.gene23407:intron 40.0%
!! TCACCAACATGCTTTGAGAA+GGG - chr8.2:62169671-62169690 MS.gene23407:intron 40.0%
AAACAGGAGGGTGACTTCTT+TGG - chr8.2:62169918-62169937 MS.gene23407:CDS 45.0%
AATTCGCAGACGGAACTTGT+TGG - chr8.2:62168043-62168062 MS.gene23407:CDS 45.0%
ACAAGTTCCGTCTGCGAATT+TGG + chr8.2:62168043-62168062 None:intergenic 45.0%
ACAGGCAAATGGAAAACAGG+AGG - chr8.2:62169905-62169924 MS.gene23407:CDS 45.0%
AGCCTATCATGCCCAGATAT+AGG + chr8.2:62168379-62168398 None:intergenic 45.0%
CACCTATATCTGGGCATGAT+AGG - chr8.2:62168374-62168393 MS.gene23407:intron 45.0%
CAGCACATGCACAAAATGCA+GGG - chr8.2:62169831-62169850 MS.gene23407:CDS 45.0%
CAGGCAAATGGAAAACAGGA+GGG - chr8.2:62169906-62169925 MS.gene23407:CDS 45.0%
TGTCACACATTCAGAGCCTT+TGG - chr8.2:62167440-62167459 MS.gene23407:CDS 45.0%
TTGCCCTTCTCAAAGCATGT+TGG + chr8.2:62169677-62169696 None:intergenic 45.0%
! TGTCTTATTGGTCTCAGGTG+GGG - chr8.2:62167943-62167962 MS.gene23407:intron 45.0%
!! AACATGCTTTGAGAAGGGCA+AGG - chr8.2:62169676-62169695 MS.gene23407:intron 45.0%
!! ACATGCTTTGAGAAGGGCAA+GGG - chr8.2:62169677-62169696 MS.gene23407:intron 45.0%
!! GTCACCAACATGCTTTGAGA+AGG - chr8.2:62169670-62169689 MS.gene23407:intron 45.0%
!! TCATCAGTTTCTGGTTTCCC+TGG + chr8.2:62167291-62167310 None:intergenic 45.0%
!! TCTCAAAGCATGTTGGTGAC+GGG + chr8.2:62169670-62169689 None:intergenic 45.0%
!! TTTGATGTTTCGGTGCTCCT+TGG + chr8.2:62167975-62167994 None:intergenic 45.0%
!!! AAAGTAATTTAAATTTTTAT+CGG + chr8.2:62168680-62168699 None:intergenic 5.0%
ACGAAGACAATCGCGTGCAA+AGG - chr8.2:62169194-62169213 MS.gene23407:CDS 50.0%
ACTCTCATCATCAGCCTTGG+TGG - chr8.2:62167358-62167377 MS.gene23407:CDS 50.0%
CCTTGGTCCAAATTCGCAGA+CGG - chr8.2:62168033-62168052 MS.gene23407:CDS 50.0%
GCAGCACATGCACAAAATGC+AGG - chr8.2:62169830-62169849 MS.gene23407:CDS 50.0%
GGTAAATGCGGTTGAGGACA+GGG + chr8.2:62168002-62168021 None:intergenic 50.0%
GTCCATGGTAAATGCGGTTG+AGG + chr8.2:62168008-62168027 None:intergenic 50.0%
GTCCTCAACCGCATTTACCA+TGG - chr8.2:62168003-62168022 MS.gene23407:intron 50.0%
TGGTAAATGCGGTTGAGGAC+AGG + chr8.2:62168003-62168022 None:intergenic 50.0%
TTGAGAAGGGCAAGGGGAAA+TGG - chr8.2:62169684-62169703 MS.gene23407:intron 50.0%
! CAGCCTTGGTGGCTTGAAAA+TGG - chr8.2:62167369-62167388 MS.gene23407:CDS 50.0%
! TGGACCAAGGTGTTTGTCCA+TGG + chr8.2:62168023-62168042 None:intergenic 50.0%
!! CATGCTTTGAGAAGGGCAAG+GGG - chr8.2:62169678-62169697 MS.gene23407:intron 50.0%
!! GAGCCATTTTCAAGCCACCA+AGG + chr8.2:62167375-62167394 None:intergenic 50.0%
AGAAGGGCAAGGGGAAATGG+CGG - chr8.2:62169687-62169706 MS.gene23407:intron 55.0%
CCGTCTGCGAATTTGGACCA+AGG + chr8.2:62168036-62168055 None:intergenic 55.0%
TGAGGGAAACAAGAGAGCCG+AGG + chr8.2:62168868-62168887 None:intergenic 55.0%
CTCAGGTGGGGACGTTGCCA+AGG - chr8.2:62167955-62167974 MS.gene23407:intron 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 62167285 62169974 62167285 ID=MS.gene23407
chr8.2 mRNA 62167285 62169974 62167285 ID=MS.gene23407.t1;Parent=MS.gene23407
chr8.2 exon 62169764 62169974 62169764 ID=MS.gene23407.t1.exon1;Parent=MS.gene23407.t1
chr8.2 CDS 62169764 62169974 62169764 ID=cds.MS.gene23407.t1;Parent=MS.gene23407.t1
chr8.2 exon 62169184 62169299 62169184 ID=MS.gene23407.t1.exon2;Parent=MS.gene23407.t1
chr8.2 CDS 62169184 62169299 62169184 ID=cds.MS.gene23407.t1;Parent=MS.gene23407.t1
chr8.2 exon 62169045 62169113 62169045 ID=MS.gene23407.t1.exon3;Parent=MS.gene23407.t1
chr8.2 CDS 62169045 62169113 62169045 ID=cds.MS.gene23407.t1;Parent=MS.gene23407.t1
chr8.2 exon 62168008 62168172 62168008 ID=MS.gene23407.t1.exon4;Parent=MS.gene23407.t1
chr8.2 CDS 62168008 62168172 62168008 ID=cds.MS.gene23407.t1;Parent=MS.gene23407.t1
chr8.2 exon 62167285 62167608 62167285 ID=MS.gene23407.t1.exon5;Parent=MS.gene23407.t1
chr8.2 CDS 62167285 62167608 62167285 ID=cds.MS.gene23407.t1;Parent=MS.gene23407.t1
Gene Sequence

>MS.gene23407

ATGCCAGGGAAACCAGAAACTGATGATTGGAGAATAAAACATAGTGAGCAGGTTCAGTTGCCATCTTCCATTTACTCTCATCATCAGCCTTGGTGGCTTGAAAATGGCTCCAAACCATCTTCAACTGATCAGCTAAATAGTTCAATCATGAATGATGTCACACATTCAGAGCCTTTGGTTTCTCCTTCACTTTCTCTAACTGATAACTCAGGTGTCTGTATTTTTATGCCTCTTCAAACTTTCTTGTTTATAGTGTATTTTTGTTTTTACTTCCTTTTGCTTACACATCTTAGCCAGCATGCTTGTGTTATCTGTAGTAATTTATCTGTTTTGATATATTAAGTGACATAATTTTAGGCCATTTGCAATTTGGTATCTAGATTTGTCAAACCAATTGGACTTTTTCATGGAAACGAACGTATAAGCATTTTACCCCCTTATTTACTATTATTGTTCATAATCAAGGGAAACTTATAGACAATTCAAGTAACTTTTTGAAGGAAATTTCACTCAATAATATTCTAGTTGTGTTCAAAGTGAGAGAATTGTGTCTTCAATGATGAATTTTGAATGATAGATTGAGAATTCTTAAGCACAAATTAAATGTGTCTCTTTACATGTATTAGCATTGTCTATGCACATAATATTCATAAAAAAATGTCTTATTGGTCTCAGGTGGGGACGTTGCCAAGGAGCACCGAAACATCAAACATGCCCTGTCCTCAACCGCATTTACCATGGACAAACACCTTGGTCCAAATTCGCAGACGGAACTTGTTGGTCATTCAATTGTAAGATCTTTATCATTACATTTTTCCAAGATATGTACACATGGTGAACTTTTGTCATATCTGGTTACAGGTTTTAACATCTCCTTATTCAAATGCACAATTCGGTCAAATCTTGAATGCTTATGGACAACAAGCTATGGTATGCTATTAAATATTGCTACGTCGATGTGATTGAAAATCAAACTTACTTGATCTAGTCTCTTTTTTAATCTATAGACTGGAACTTACCTTCTTTTTATTCATGGGAAAGATAATTTGTTTATTTCTTGTGGTTGGTGTCTAGGATAGATATTGTGTGCACCTATATCTGGGCATGATAGGCTTATTCGCAACATAAAATACAATCAGCATATTCATTTATTCCTTGAAAGGACATTGCATATTAATATTTATAATTATTACAAATACTATCAACAAATATGCTTGTTTGATTTTTTATTTTTTTTTACAAATATGTGGTAATGATAAAATAGTTCGTAAGGAGAATATGCGTGTAATCTATGAAGCCCAGATACTTCAGAAATAGAAGAGTATCAGTGTCCGGCACATATTCGATAGCGATACACATATGATACTATCTGATACGTACTAAAAAAAATATCCGATAAAAATTTAAATTACTTTTAAAGATACTTTCACCGATATTATGTGTTGATTGCTTGCATGTTCTTAATTGTGTGTAGATTAATCTCAATAGTAATACTTGTTACTCATTCAGTTTGAAGTAATTTGACCATGTTAGTACTTAGTAATTGTAACAACAAGCTTGAATGTATCTTTAGCTGTAAGCCTCGGCTCTCTTGTTTCCCTCATTACCTTTTGCACAAGATTCATCTTTTCTCTGTATGGACTTTTGCATCTAAATTTAAATTAAACCTCTCTTTCTCTTCCTTTTTTCGTCCAAGACGTTTTTTATTTTTAAGGGCCTATCAGTCTTTTAATATTGTTAATAGTTAATACTGCTCTGTTGCTAATTTACTCAAGTCCTAACAGCTATTGGCATTATGCTAGATGAACCCTCAGCTATACCAAATGCATCATGCTAGAATGCTTTTGCCGCTTAAAATGGAAGAGGAACCTGTGTATGTCAATGCAAAGCAGTATCATGGTATTTTGAGACGAAGACAATCGCGTGCAAAGGCTGAGCTTGAAAAGAAAGTTATTAAAGTTAGAAAGGTATGACTGATATCTACCTTATAGCATTATCATTGATATGATGAAAAAAATGGATGTAAAATTAAAATTCATTGCGTTTTATACTTGACTATCATATTTGTGTATCTTTTGATATCCAAAACCAACTTTCCTAAAAGATTAAGTAGTTTGGAAAAGGCACCTCAAGAGTTTTATATTAAGAAATATGTAAAATATCTTATACTATCTTAATTGTATCTTGGATCAATCTAGGGTAACTTAATTAGCCCTTGGGAATGGTTTCTATATTTTATCATTTATCATTTTTTTTCTCTTATTCGATGAAGATTAGGACTCTAGTACTAGTATAAATATGTATTAATGCTTTTCCATTTGTGTAGAATTGATCATAGTCACAGTTGTATCATTCATTTCTAACAGCCATATCTTCATGAATCCCGTCACCAACATGCTTTGAGAAGGGCAAGGGGAAATGGCGGTCGTTTCCTCAATACAAAGAAGCTCGAACACAACGATTCTAATGCTGCTCTAGAAAAAGGCAACAATACTGGTGCAACATCGGGTACCAACAATGAGAATCAAGGCTCATCTAATGTGTCGCAGCACATGCACAAAATGCAGGGTTTCAACATCAGTTACCATGATGGAAATGACTTTACAGCACTATGTCATTCACAGGCAAATGGAAAACAGGAGGGTGACTTCTTTGGTAAAAAGATTGAATCAAATGGTGCTATTAAATGA
Protein sequence

>MS.gene23407.t1

MPGKPETDDWRIKHSEQVQLPSSIYSHHQPWWLENGSKPSSTDQLNSSIMNDVTHSEPLVSPSLSLTDNSGGDVAKEHRNIKHALSSTAFTMDKHLGPNSQTELVGHSIVLTSPYSNAQFGQILNAYGQQAMMNPQLYQMHHARMLLPLKMEEEPVYVNAKQYHGILRRRQSRAKAELEKKVIKVRKPYLHESRHQHALRRARGNGGRFLNTKKLEHNDSNAALEKGNNTGATSGTNNENQGSSNVSQHMHKMQGFNISYHDGNDFTALCHSQANGKQEGDFFGKKIESNGAIK