AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056202

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056202.t1 MTR_8g038250 89.066 439 24 2 1 438 1 416 0.0 779
MS.gene056202.t1 MTR_8g096990 78.995 438 86 2 1 438 1 432 0.0 710
MS.gene056202.t1 MTR_5g080890 73.923 441 105 6 1 438 1 434 0.0 647
MS.gene056202.t1 MTR_8g038280 71.719 442 115 3 1 438 1 436 0.0 647
MS.gene056202.t1 MTR_5g080960 73.696 441 106 6 1 438 1 434 0.0 645
MS.gene056202.t1 MTR_5g044770 70.548 438 122 3 1 438 1 431 0.0 619
MS.gene056202.t1 MTR_6g090290 64.706 442 145 4 1 437 1 436 0.0 554
MS.gene056202.t1 MTR_8g038310 61.187 438 144 5 1 437 1 413 0.0 532
MS.gene056202.t1 MTR_4g109760 65.148 439 112 7 1 438 1 399 0.0 516
MS.gene056202.t1 MTR_3g028120 53.333 420 186 6 25 438 24 439 4.73e-149 431
MS.gene056202.t1 MTR_5g038360 50.797 439 202 7 5 437 6 436 2.12e-141 411
MS.gene056202.t1 MTR_2g067830 52.913 412 182 7 32 437 1 406 8.35e-138 401
MS.gene056202.t1 MTR_6g088760 52.956 406 175 8 1 395 1 401 1.78e-136 402
MS.gene056202.t1 MTR_5g044570 48.090 445 189 10 1 437 1 411 6.26e-129 379
MS.gene056202.t1 MTR_0024s0040 50.000 418 191 9 30 437 1 410 1.21e-125 370
MS.gene056202.t1 MTR_2g105300 49.275 414 166 8 32 434 1 381 3.08e-123 363
MS.gene056202.t1 MTR_0036s0050 44.917 423 208 10 26 437 27 435 3.87e-112 337
MS.gene056202.t1 MTR_0036s0070 42.045 440 226 12 9 437 5 426 2.88e-107 324
MS.gene056202.t1 MTR_0036s0110 40.716 447 238 11 6 437 8 442 2.94e-100 306
MS.gene056202.t1 MTR_5g008070 41.469 422 222 10 21 436 23 425 1.41e-96 296
MS.gene056202.t1 MTR_2g059490 40.233 430 219 9 14 438 15 411 2.11e-91 284
MS.gene056202.t1 MTR_2g059460 40.284 422 205 10 23 437 25 406 6.70e-91 281
MS.gene056202.t1 MTR_5g008150 39.573 422 213 11 22 437 27 412 3.63e-89 277
MS.gene056202.t1 MTR_5g008110 38.288 444 221 12 7 437 12 415 1.54e-88 275
MS.gene056202.t1 MTR_3g097150 36.706 425 239 15 27 438 42 449 3.15e-75 242
MS.gene056202.t1 MTR_1g060750 37.778 405 215 12 40 437 2 376 6.35e-74 236
MS.gene056202.t1 MTR_4g045857 38.907 311 155 6 6 312 9 288 3.21e-60 198
MS.gene056202.t1 MTR_5g008090 37.086 302 176 8 142 437 21 314 2.12e-56 189
MS.gene056202.t1 MTR_7g117430 34.404 436 236 18 25 435 74 484 1.35e-54 189
MS.gene056202.t1 MTR_1g115210 35.635 362 202 14 85 435 145 486 1.63e-53 186
MS.gene056202.t1 MTR_1g039270 34.341 364 205 16 85 435 127 469 8.22e-50 176
MS.gene056202.t1 MTR_3g046300 29.252 441 268 11 30 438 33 461 6.35e-47 168
MS.gene056202.t1 MTR_6g085010 34.648 355 208 13 86 435 156 491 2.02e-46 167
MS.gene056202.t1 MTR_1g111350 33.983 359 208 12 85 435 130 467 3.33e-46 166
MS.gene056202.t1 MTR_6g088765 40.756 238 107 5 163 399 5 209 1.87e-44 155
MS.gene056202.t1 MTR_5g091130 31.538 390 242 11 68 438 173 556 4.64e-42 156
MS.gene056202.t1 MTR_2g028190 31.343 402 247 13 49 435 64 451 4.11e-40 149
MS.gene056202.t1 MTR_4g007740 31.694 366 214 13 87 436 133 478 2.46e-39 147
MS.gene056202.t1 MTR_3g053710 32.507 363 221 12 85 435 116 466 3.66e-39 147
MS.gene056202.t1 MTR_2g070430 28.481 474 276 20 5 437 6 457 3.68e-36 138
MS.gene056202.t1 MTR_8g103880 27.979 386 252 13 59 436 89 456 4.66e-36 138
MS.gene056202.t1 MTR_2g055840 29.581 382 232 12 78 435 5 373 1.61e-35 135
MS.gene056202.t1 MTR_7g080230 27.727 440 266 17 30 436 11 431 2.84e-35 136
MS.gene056202.t1 MTR_1g078240 29.319 382 221 15 85 436 68 430 5.08e-32 127
MS.gene056202.t1 MTR_1g078240 29.765 383 219 16 85 436 68 431 1.87e-31 125
MS.gene056202.t1 MTR_7g105850 28.838 482 269 22 9 437 7 467 2.20e-31 125
MS.gene056202.t1 MTR_3g068000 29.243 383 218 15 89 435 76 441 7.28e-31 124
MS.gene056202.t1 MTR_7g021510 29.124 388 222 19 82 436 67 434 2.32e-30 122
MS.gene056202.t1 MTR_3g065160 26.119 402 250 13 67 439 47 430 7.84e-30 120
MS.gene056202.t1 MTR_4g008110 55.752 113 27 1 162 274 48 137 1.97e-29 112
MS.gene056202.t1 MTR_4g095270 25.359 418 264 17 41 438 37 426 1.51e-27 115
MS.gene056202.t1 MTR_4g095270 25.359 418 264 17 41 438 37 426 2.07e-27 115
MS.gene056202.t1 MTR_3g053980 31.680 363 218 13 85 435 141 485 2.61e-27 114
MS.gene056202.t1 MTR_8g094590 27.851 377 228 14 85 438 72 427 3.16e-27 113
MS.gene056202.t1 MTR_5g077850 25.984 381 229 14 88 438 83 440 3.24e-26 110
MS.gene056202.t1 MTR_3g096930 25.258 388 243 13 78 438 70 437 4.45e-26 110
MS.gene056202.t1 MTR_4g056420 29.365 378 225 15 85 436 76 437 5.13e-26 110
MS.gene056202.t1 MTR_5g016260 24.574 411 252 16 49 438 61 434 1.74e-25 109
MS.gene056202.t1 MTR_7g062360 27.604 384 227 16 89 438 62 428 2.22e-25 108
MS.gene056202.t1 MTR_5g016260 24.814 403 245 16 57 438 69 434 2.29e-25 109
MS.gene056202.t1 MTR_2g063110 24.895 478 275 19 28 435 45 508 4.12e-25 107
MS.gene056202.t1 MTR_7g080230 28.045 353 209 15 110 436 28 361 4.75e-25 106
MS.gene056202.t1 MTR_1g029810 25.532 376 239 13 85 438 56 412 8.30e-25 106
MS.gene056202.t1 MTR_1g029810 25.532 376 239 13 85 438 56 412 1.10e-24 105
MS.gene056202.t1 MTR_4g023630 26.190 420 259 16 50 438 23 422 2.41e-24 104
MS.gene056202.t1 MTR_1g078240 29.747 316 176 13 85 373 68 364 8.40e-24 102
MS.gene056202.t1 MTR_6g084710 27.366 391 234 17 80 439 186 557 1.13e-23 103
MS.gene056202.t1 MTR_8g039540 28.140 430 273 17 12 423 10 421 1.58e-23 102
MS.gene056202.t1 MTR_4g023650 25.891 421 259 17 50 438 23 422 2.03e-23 102
MS.gene056202.t1 MTR_8g094590 31.907 257 149 9 85 334 72 309 2.81e-23 100
MS.gene056202.t1 MTR_2g103340 36.813 182 65 4 20 199 33 166 2.89e-23 97.1
MS.gene056202.t1 MTR_2g103340 43.820 89 40 3 351 439 88 166 1.58e-11 63.5
MS.gene056202.t1 MTR_3g014290 30.612 343 213 13 87 423 81 404 9.37e-23 100
MS.gene056202.t1 MTR_1g037630 27.603 413 263 14 25 423 26 416 1.03e-22 100
MS.gene056202.t1 MTR_8g094590 30.827 266 158 9 85 343 72 318 1.44e-22 99.4
MS.gene056202.t1 MTR_2g084550 29.737 380 222 16 85 436 76 438 1.73e-22 100
MS.gene056202.t1 MTR_1g061940 28.272 382 228 16 84 436 81 445 2.49e-22 99.8
MS.gene056202.t1 MTR_0945s0010 38.760 129 74 4 311 438 1 125 3.04e-22 93.2
MS.gene056202.t1 MTR_7g102750 27.553 421 237 22 59 436 46 441 2.61e-21 96.7
MS.gene056202.t1 MTR_6g084710 29.392 296 180 14 80 365 186 462 8.04e-21 95.1
MS.gene056202.t1 MTR_4g100990 27.338 417 222 21 86 438 74 473 1.93e-20 94.0
MS.gene056202.t1 MTR_5g061690 29.296 355 198 20 93 423 108 433 5.72e-19 89.7
MS.gene056202.t1 MTR_8g075090 27.559 381 218 19 87 439 113 463 2.63e-18 87.4
MS.gene056202.t1 MTR_8g075090 27.441 379 217 19 87 437 113 461 6.75e-18 86.3
MS.gene056202.t1 MTR_7g102750 28.134 359 200 18 59 379 46 384 1.10e-17 85.1
MS.gene056202.t1 MTR_5g077920 27.005 374 234 13 88 437 70 428 2.35e-17 84.3
MS.gene056202.t1 MTR_8g075090 29.605 304 172 14 92 374 118 400 8.58e-17 82.8
MS.gene056202.t1 MTR_8g075010 25.916 382 223 18 87 438 101 452 4.73e-15 77.4
MS.gene056202.t1 MTR_5g080330 25.344 363 199 17 103 435 2 322 4.78e-15 77.0
MS.gene056202.t1 MTR_2g037710 27.189 217 125 7 228 436 15 206 8.37e-14 70.5
MS.gene056202.t1 MTR_3g100500 27.889 398 245 19 44 422 50 424 1.99e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056202.t1 AT1G64830 50.966 414 196 3 26 438 24 431 2.14e-138 404
MS.gene056202.t1 AT5G33340 52.771 415 186 5 27 438 30 437 1.99e-135 396
MS.gene056202.t1 AT2G35615 45.275 455 225 8 1 439 1 447 2.96e-116 348
MS.gene056202.t1 AT1G31450 43.304 448 225 9 4 436 9 442 3.34e-105 319
MS.gene056202.t1 AT2G28010 40.868 438 206 12 5 438 6 394 1.30e-92 285
MS.gene056202.t1 AT2G28040 41.315 426 197 12 16 438 18 393 1.15e-89 278
MS.gene056202.t1 AT2G28040 41.827 416 189 12 26 438 19 384 1.63e-88 275
MS.gene056202.t1 AT2G28030 43.750 368 177 11 75 438 49 390 9.03e-85 265
MS.gene056202.t1 AT2G28220 38.605 430 212 11 15 438 17 400 2.43e-83 261
MS.gene056202.t1 AT2G28225 40.970 371 187 9 75 438 55 400 2.47e-78 249
MS.gene056202.t1 AT2G03200 36.384 437 238 14 25 438 42 461 1.23e-69 228
MS.gene056202.t1 AT1G01300 38.017 363 197 14 85 436 140 485 2.15e-54 188
MS.gene056202.t1 AT3G61820 36.616 396 216 15 55 435 108 483 4.14e-54 187
MS.gene056202.t1 AT3G18490 32.589 448 268 15 1 435 74 500 4.46e-54 188
MS.gene056202.t1 AT4G30030 36.658 371 206 15 79 438 70 422 1.21e-52 182
MS.gene056202.t1 AT5G10760 32.634 429 251 14 23 435 58 464 1.09e-47 170
MS.gene056202.t1 AT2G23945 28.160 451 294 12 9 438 10 451 6.28e-47 168
MS.gene056202.t1 AT1G25510 33.973 365 200 15 85 435 146 483 2.05e-46 167
MS.gene056202.t1 AT5G36260 31.926 379 211 15 85 436 76 434 8.39e-45 162
MS.gene056202.t1 AT3G20015 30.248 443 243 18 28 435 59 470 1.49e-44 162
MS.gene056202.t1 AT3G25700 30.324 432 247 17 39 436 39 450 2.47e-44 160
MS.gene056202.t1 AT5G10770 32.719 434 246 17 29 436 61 474 1.50e-42 156
MS.gene056202.t1 AT3G02740 30.933 375 219 14 85 436 83 440 1.95e-40 151
MS.gene056202.t1 AT3G02740 30.295 373 220 13 87 436 10 365 2.60e-40 149
MS.gene056202.t1 AT4G30040 30.310 452 251 21 9 430 10 427 1.90e-39 147
MS.gene056202.t1 AT1G65240 30.952 378 213 15 85 436 72 427 1.19e-38 145
MS.gene056202.t1 AT2G42980 32.481 391 232 14 72 438 145 527 1.47e-38 146
MS.gene056202.t1 AT3G59080 31.538 390 235 15 72 438 155 535 2.25e-38 146
MS.gene056202.t1 AT3G12700 27.473 455 288 15 5 436 26 461 9.51e-38 143
MS.gene056202.t1 AT1G79720 33.151 365 211 15 87 435 135 482 6.28e-36 138
MS.gene056202.t1 AT2G39710 29.521 376 214 15 89 430 67 425 4.92e-33 129
MS.gene056202.t1 AT5G37540 28.267 375 225 14 88 435 81 438 7.94e-32 126
MS.gene056202.t1 AT3G59080 29.793 386 211 14 72 438 155 499 1.74e-30 123
MS.gene056202.t1 AT1G66180 26.455 378 232 14 88 439 73 430 1.88e-30 122
MS.gene056202.t1 AT4G33490 25.000 412 250 13 61 438 28 414 2.86e-29 119
MS.gene056202.t1 AT3G54400 28.093 388 250 13 61 436 55 425 9.04e-28 114
MS.gene056202.t1 AT5G43100 26.932 427 255 21 37 438 26 420 9.41e-28 115
MS.gene056202.t1 AT5G02190 27.128 376 223 15 96 435 82 442 1.37e-27 114
MS.gene056202.t1 AT5G43100 26.932 427 255 21 37 438 26 420 1.87e-27 115
MS.gene056202.t1 AT1G05840 28.000 375 234 15 85 436 78 439 2.20e-27 114
MS.gene056202.t1 AT3G50050 26.144 459 275 22 12 438 13 439 7.64e-26 110
MS.gene056202.t1 AT1G44130 25.750 400 249 14 67 438 23 402 1.42e-25 108
MS.gene056202.t1 AT5G45120 28.814 413 213 19 87 435 83 478 2.56e-25 108
MS.gene056202.t1 AT4G12920 27.273 385 241 14 61 435 32 387 4.66e-25 106
MS.gene056202.t1 AT1G09750 28.997 369 222 16 85 435 102 448 5.48e-25 107
MS.gene056202.t1 AT4G33490 28.525 305 177 11 61 349 25 304 8.61e-24 103
MS.gene056202.t1 AT5G22850 28.760 379 227 14 85 436 79 441 1.13e-23 103
MS.gene056202.t1 AT2G36670 27.273 418 245 19 59 436 60 458 4.55e-23 102
MS.gene056202.t1 AT1G77480 26.371 383 228 17 85 438 65 422 1.43e-22 99.8
MS.gene056202.t1 AT1G77480 26.371 383 228 17 85 438 65 422 1.98e-22 99.8
MS.gene056202.t1 AT2G36670 26.950 423 245 19 59 436 60 463 2.21e-22 100
MS.gene056202.t1 AT2G36670 27.467 375 231 15 87 436 34 392 4.24e-22 98.6
MS.gene056202.t1 AT2G36670 27.467 375 231 15 87 436 34 392 4.24e-22 98.6
MS.gene056202.t1 AT2G17760 27.546 432 249 20 32 435 54 449 6.54e-22 98.6
MS.gene056202.t1 AT3G52500 29.219 397 222 21 84 436 87 468 5.16e-21 95.5
MS.gene056202.t1 AT1G49050 24.931 361 230 12 105 437 50 397 1.63e-20 93.6
MS.gene056202.t1 AT1G49050 25.641 390 242 15 80 437 197 570 2.14e-20 94.4
MS.gene056202.t1 AT1G08210 27.851 377 230 15 85 436 130 489 6.04e-20 92.8
MS.gene056202.t1 AT1G08210 27.851 377 230 15 85 436 116 475 6.46e-20 92.4
MS.gene056202.t1 AT5G07030 27.371 464 285 18 2 436 15 455 7.02e-20 92.0
MS.gene056202.t1 AT1G08210 27.851 377 230 15 85 436 82 441 7.04e-20 92.4
MS.gene056202.t1 AT3G51350 24.888 446 275 18 27 435 30 452 5.12e-19 89.7
MS.gene056202.t1 AT1G49050 27.957 279 168 12 80 341 197 459 8.50e-18 85.9
MS.gene056202.t1 AT3G42550 26.263 396 227 16 55 436 46 390 6.34e-17 82.8
MS.gene056202.t1 AT1G08210 26.551 403 229 16 85 436 82 468 6.61e-16 80.1
MS.gene056202.t1 AT3G25700 29.082 196 113 7 39 222 39 220 1.93e-15 77.8
MS.gene056202.t1 AT5G10080 25.156 481 279 27 8 437 5 455 3.12e-14 75.1
MS.gene056202.t1 AT3G51360 24.078 461 286 19 10 435 4 435 6.27e-14 73.9
MS.gene056202.t1 AT3G51360 24.359 468 288 21 6 436 8 446 6.30e-14 73.9
MS.gene056202.t1 AT5G24820 24.745 392 240 16 74 435 33 399 1.03e-13 73.2
MS.gene056202.t1 AT3G51330 25.745 369 227 18 92 435 107 453 3.66e-12 68.6
MS.gene056202.t1 AT3G51330 25.745 369 227 18 92 435 107 453 4.00e-12 68.6
MS.gene056202.t1 AT3G51340 23.975 317 208 11 59 357 72 373 2.19e-11 65.9
MS.gene056202.t1 AT3G51340 23.824 319 210 11 59 359 72 375 4.17e-11 65.5

Find 88 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTGGTGTTTATAATAATT+TGG 0.122254 8.1:-65544335 MS.gene056202:CDS
GGAGATGCCGCTGTGGTTTC+CGG 0.148862 8.1:-65544529 MS.gene056202:CDS
TTAAAATGTGCCGTTATTAT+AGG 0.181038 8.1:+65544214 None:intergenic
GGTTCTGACCTTTCCATCTT+TGG 0.183882 8.1:-65544097 MS.gene056202:CDS
ACGACAAGCAAACTCAATTT+TGG 0.203851 8.1:-65544550 MS.gene056202:CDS
ACCCCACCATTTAAGTTATA+TGG 0.242744 8.1:-65545021 MS.gene056202:CDS
GCAATGCCATATAACTTAAA+TGG 0.273035 8.1:+65545015 None:intergenic
AACAAAAGAATAGAATTTAA+AGG 0.289730 8.1:-65544424 MS.gene056202:CDS
TCATTTCCTAAAACTGTGAT+AGG 0.290097 8.1:-65544724 MS.gene056202:CDS
GAGATGCCGCTGTGGTTTCC+GGG 0.323952 8.1:-65544528 MS.gene056202:CDS
CTTGTCGTGTTTGACTCAAC+CGG 0.337489 8.1:+65544565 None:intergenic
ACAGCAACAAATAAACTAAT+AGG 0.342697 8.1:+65544169 None:intergenic
AGCGTCTCTTATTACACAAT+TGG 0.347914 8.1:-65544632 MS.gene056202:CDS
GGTAACATCATAATTGATTC+TGG 0.364728 8.1:-65544379 MS.gene056202:CDS
ATACAGGTAGTGACATTGTT+TGG 0.380232 8.1:-65544990 MS.gene056202:CDS
TATAGCAAAGGTTAAATTCT+CGG 0.382010 8.1:+65544263 None:intergenic
CTTAGCTCAGCAGAATTTGT+TGG 0.389893 8.1:-65544071 MS.gene056202:CDS
GCAGTTACTTCTGGGTTCCC+TGG 0.393771 8.1:-65544118 MS.gene056202:CDS
AGCTCCGGTGTAGTTGGCCT+TGG 0.399332 8.1:-65544664 MS.gene056202:CDS
GCCGCTGTGGTTTCCGGGGA+TGG 0.421556 8.1:-65544523 MS.gene056202:CDS
GTCAAATAGTAGAAAACTAT+AGG 0.426026 8.1:+65544469 None:intergenic
GTGTTTGACTCAACCGGGTT+TGG 0.428845 8.1:+65544571 None:intergenic
GGAATCAGCTGTGTTGGAAT+TGG 0.432066 8.1:-65544314 MS.gene056202:CDS
ATAGCAAAGGTTAAATTCTC+GGG 0.433218 8.1:+65544264 None:intergenic
TCCGGTGTAGTTGGCCTTGG+AGG 0.448012 8.1:-65544661 MS.gene056202:CDS
ACAAAAGAATAGAATTTAAA+GGG 0.448273 8.1:-65544423 MS.gene056202:CDS
CTGAGCTAAGTTTCCAAAGA+TGG 0.451967 8.1:+65544084 None:intergenic
ATGAGATATTCACCATGATC+GGG 0.455002 8.1:+65545060 None:intergenic
GACAATACAATGTCATTTGA+AGG 0.463678 8.1:-65544691 MS.gene056202:CDS
AAAGAGAAATAATGAAACAA+AGG 0.467908 8.1:+65545265 None:intergenic
GCGTCTCTTATTACACAATT+GGG 0.469339 8.1:-65544631 MS.gene056202:CDS
ACGTTGACAGTTATTCCAAC+TGG 0.472429 8.1:-65544352 MS.gene056202:CDS
CCTAAAACTGTGATAGGATG+TGG 0.472735 8.1:-65544718 MS.gene056202:CDS
AAGAGAAATAATGAAACAAA+GGG 0.474915 8.1:+65545266 None:intergenic
TAATTTGGAATCAGCTGTGT+TGG 0.477151 8.1:-65544320 MS.gene056202:CDS
AAATTATTATAAACACCAGT+TGG 0.480158 8.1:+65544337 None:intergenic
TTATATGGCATTGCTGATAC+AGG 0.486441 8.1:-65545006 MS.gene056202:CDS
GAATTTAAAGGGTCTTCAAA+TGG 0.487908 8.1:-65544412 MS.gene056202:CDS
GGTGTAGTTGGCCTTGGAGG+TGG 0.491484 8.1:-65544658 MS.gene056202:CDS
GATTGGCACGGTTTCTAGAA+CGG 0.494681 8.1:+65545130 None:intergenic
CTTGTTGCACAGATTTACAT+AGG 0.501571 8.1:+65544869 None:intergenic
GGTGCAAGCTCCGGTGTAGT+TGG 0.509113 8.1:-65544670 MS.gene056202:CDS
ACCTCCAAGGCCAACTACAC+CGG 0.511689 8.1:+65544660 None:intergenic
TGCCATATAACTTAAATGGT+GGG 0.513388 8.1:+65545019 None:intergenic
GGTGATACGTCACAATCAAA+AGG 0.514921 8.1:-65544799 MS.gene056202:CDS
CAAAGGTTAAATTCTCGGGT+TGG 0.519243 8.1:+65544268 None:intergenic
TTATTGTTTGTTGCCAAACC+CGG 0.519426 8.1:-65544584 MS.gene056202:CDS
TGATTGTAACATTCTTTGCA+AGG 0.519730 8.1:+65544955 None:intergenic
TCATTTGAAGGTGCAAGCTC+CGG 0.527243 8.1:-65544679 MS.gene056202:CDS
CATGAGATATTCACCATGAT+CGG 0.529684 8.1:+65545059 None:intergenic
TTGTCGTGTTTGACTCAACC+GGG 0.535482 8.1:+65544566 None:intergenic
TGCTATAGTATCACATCAGA+TGG 0.537260 8.1:-65544247 MS.gene056202:CDS
GTCTTCAAATGGTGGTGATG+AGG 0.541942 8.1:-65544401 MS.gene056202:CDS
TTATTTGTTGCTGTTGCAGA+TGG 0.545033 8.1:-65544160 MS.gene056202:CDS
TCAACTGTAATCCCCGATCA+TGG 0.547516 8.1:-65545072 MS.gene056202:CDS
GAGACGCTGGGCCACCTCCA+AGG 0.553284 8.1:+65544647 None:intergenic
CTTTCTCATGCTTTAAACAA+CGG 0.553785 8.1:-65545246 MS.gene056202:CDS
ACATTGGAAGCATTTAGTGT+TGG 0.556371 8.1:-65544448 MS.gene056202:CDS
AAGATGGAAAGGTCAGAACC+AGG 0.560391 8.1:+65544100 None:intergenic
GCTCAGCAGAATTTGTTGGT+TGG 0.563160 8.1:-65544067 MS.gene056202:CDS
TCTTCAAATGGTGGTGATGA+GGG 0.564161 8.1:-65544400 MS.gene056202:CDS
ATGCCATATAACTTAAATGG+TGG 0.567034 8.1:+65545018 None:intergenic
TTGATTGAGGTGTGTTTGTG+AGG 0.568393 8.1:+65545091 None:intergenic
TCGGGGATTACAGTTGATTG+AGG 0.568413 8.1:+65545078 None:intergenic
CTAAGTTTCCAAAGATGGAA+AGG 0.568814 8.1:+65544089 None:intergenic
AATTGTGTAATAAGAGACGC+TGG 0.577042 8.1:+65544634 None:intergenic
GCCATATAACTTAAATGGTG+GGG 0.578953 8.1:+65545020 None:intergenic
GTGCGGCATTGACAATATGT+TGG 0.580612 8.1:+65545154 None:intergenic
GGTTGACACTACACCATCCC+CGG 0.582467 8.1:+65544510 None:intergenic
AACAAAGGGAGAAGTAAAAG+AGG 0.583827 8.1:+65545280 None:intergenic
TTTAAAGGGTCTTCAAATGG+TGG 0.588943 8.1:-65544409 MS.gene056202:CDS
TGATGGTTTAAACTTAGGAG+TGG 0.593505 8.1:+65544930 None:intergenic
TGATTGAGGTGTGTTTGTGA+GGG 0.596814 8.1:+65545092 None:intergenic
TATCTCATGACATATTCAGT+TGG 0.603094 8.1:-65545045 MS.gene056202:CDS
AACACTTTGCTACAATCAGT+AGG 0.606762 8.1:+65544010 None:intergenic
CTGATGTGATACTATAGCAA+AGG 0.615704 8.1:+65544251 None:intergenic
CTTACATTAGAGTCCTCCAC+TGG 0.621526 8.1:-65544757 MS.gene056202:CDS
AAATGAAATAGGACGTCCAG+TGG 0.627128 8.1:+65544741 None:intergenic
AGATGCCGCTGTGGTTTCCG+GGG 0.633627 8.1:-65544527 MS.gene056202:CDS
TTACATAGGTCAGACGAACA+TGG 0.633649 8.1:+65544883 None:intergenic
AGATGGAAAGGTCAGAACCA+GGG 0.650286 8.1:+65544101 None:intergenic
TGAAATAGGACGTCCAGTGG+AGG 0.654304 8.1:+65544744 None:intergenic
GAAGTAAAAGAGGCTCAGCA+AGG 0.655704 8.1:+65545290 None:intergenic
TGAGATATTCACCATGATCG+GGG 0.667290 8.1:+65545061 None:intergenic
ACGGTTTCTAGAACGGCGTG+CGG 0.669771 8.1:+65545137 None:intergenic
CTATGTAAATCTGTGCAACA+AGG 0.670936 8.1:-65544868 MS.gene056202:CDS
ATTGTGTAATAAGAGACGCT+GGG 0.697269 8.1:+65544635 None:intergenic
ACCATCCCCGGAAACCACAG+CGG 0.698241 8.1:+65544522 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAATTGTCATTACAAT+CGG + chr8.1:65544469-65544488 None:intergenic 15.0%
!! AACAAAAGAATAGAATTTAA+AGG - chr8.1:65544885-65544904 MS.gene056202:CDS 15.0%
!! ACAAAAGAATAGAATTTAAA+GGG - chr8.1:65544886-65544905 MS.gene056202:CDS 15.0%
!!! ATATGATTTTCCTATAATAA+CGG - chr8.1:65545085-65545104 MS.gene056202:CDS 15.0%
!!! TGTGAATATAGTATTTTTTA+TGG - chr8.1:65544489-65544508 MS.gene056202:CDS 15.0%
!! AAAGAGAAATAATGAAACAA+AGG + chr8.1:65544047-65544066 None:intergenic 20.0%
!! AAATTATTATAAACACCAGT+TGG + chr8.1:65544975-65544994 None:intergenic 20.0%
!! AACTGGTGTTTATAATAATT+TGG - chr8.1:65544974-65544993 MS.gene056202:CDS 20.0%
!! AAGAGAAATAATGAAACAAA+GGG + chr8.1:65544046-65544065 None:intergenic 20.0%
! ACAGCAACAAATAAACTAAT+AGG + chr8.1:65545143-65545162 None:intergenic 25.0%
! GTCAAATAGTAGAAAACTAT+AGG + chr8.1:65544843-65544862 None:intergenic 25.0%
! TATAGCAAAGGTTAAATTCT+CGG + chr8.1:65545049-65545068 None:intergenic 25.0%
!! AGTTTTCTACTATTTGACAT+TGG - chr8.1:65544845-65544864 MS.gene056202:CDS 25.0%
!! ATAATAACGGCACATTTTAA+AGG - chr8.1:65545098-65545117 MS.gene056202:CDS 25.0%
!! TTAAAATGTGCCGTTATTAT+AGG + chr8.1:65545098-65545117 None:intergenic 25.0%
!!! ACAGTTTTAGGAAATGAAAT+AGG + chr8.1:65544582-65544601 None:intergenic 25.0%
!!! ATAGGATCCTTTTTTACTAT+AGG + chr8.1:65544825-65544844 None:intergenic 25.0%
!!! GATTTTGATGGTTTAAACTT+AGG + chr8.1:65544387-65544406 None:intergenic 25.0%
!!! TAGGATCCTTTTTTACTATA+GGG + chr8.1:65544824-65544843 None:intergenic 25.0%
ATAGCAAAGGTTAAATTCTC+GGG + chr8.1:65545048-65545067 None:intergenic 30.0%
ATGCCATATAACTTAAATGG+TGG + chr8.1:65544294-65544313 None:intergenic 30.0%
GAATTTAAAGGGTCTTCAAA+TGG - chr8.1:65544897-65544916 MS.gene056202:CDS 30.0%
GCAATGCCATATAACTTAAA+TGG + chr8.1:65544297-65544316 None:intergenic 30.0%
TATCTCATGACATATTCAGT+TGG - chr8.1:65544264-65544283 MS.gene056202:CDS 30.0%
TCATTTCCTAAAACTGTGAT+AGG - chr8.1:65544585-65544604 MS.gene056202:CDS 30.0%
TGATTGTAACATTCTTTGCA+AGG + chr8.1:65544357-65544376 None:intergenic 30.0%
TGCCATATAACTTAAATGGT+GGG + chr8.1:65544293-65544312 None:intergenic 30.0%
! CTTTCTCATGCTTTAAACAA+CGG - chr8.1:65544063-65544082 MS.gene056202:CDS 30.0%
! GACAATACAATGTCATTTGA+AGG - chr8.1:65544618-65544637 MS.gene056202:CDS 30.0%
! GGTAACATCATAATTGATTC+TGG - chr8.1:65544930-65544949 MS.gene056202:CDS 30.0%
! TTTTGTAGAAATGATTGGCA+CGG + chr8.1:65544194-65544213 None:intergenic 30.0%
!! TTGTAAGTTGACGATTTTGA+TGG + chr8.1:65544399-65544418 None:intergenic 30.0%
!!! ACGACACAATTTTTTGTTGA+AGG + chr8.1:65545274-65545293 None:intergenic 30.0%
!!! AGGATCCTTTTTTACTATAG+GGG + chr8.1:65544823-65544842 None:intergenic 30.0%
!!! TGTTGGTATTTGTTTTGTGT+AGG + chr8.1:65544141-65544160 None:intergenic 30.0%
AACAAAGGGAGAAGTAAAAG+AGG + chr8.1:65544032-65544051 None:intergenic 35.0%
AACACTTTGCTACAATCAGT+AGG + chr8.1:65545302-65545321 None:intergenic 35.0%
AATTGTGTAATAAGAGACGC+TGG + chr8.1:65544678-65544697 None:intergenic 35.0%
AGCGTCTCTTATTACACAAT+TGG - chr8.1:65544677-65544696 MS.gene056202:CDS 35.0%
ATACAGGTAGTGACATTGTT+TGG - chr8.1:65544319-65544338 MS.gene056202:CDS 35.0%
ATGAGATATTCACCATGATC+GGG + chr8.1:65544252-65544271 None:intergenic 35.0%
ATTGTGTAATAAGAGACGCT+GGG + chr8.1:65544677-65544696 None:intergenic 35.0%
CATGAGATATTCACCATGAT+CGG + chr8.1:65544253-65544272 None:intergenic 35.0%
CTAAGTTTCCAAAGATGGAA+AGG + chr8.1:65545223-65545242 None:intergenic 35.0%
CTATGTAAATCTGTGCAACA+AGG - chr8.1:65544441-65544460 MS.gene056202:CDS 35.0%
CTGATGTGATACTATAGCAA+AGG + chr8.1:65545061-65545080 None:intergenic 35.0%
CTTGTTGCACAGATTTACAT+AGG + chr8.1:65544443-65544462 None:intergenic 35.0%
GCCATATAACTTAAATGGTG+GGG + chr8.1:65544292-65544311 None:intergenic 35.0%
GCGTCTCTTATTACACAATT+GGG - chr8.1:65544678-65544697 MS.gene056202:CDS 35.0%
GTCAACCCCTATAGTAAAAA+AGG - chr8.1:65544815-65544834 MS.gene056202:CDS 35.0%
TGATGGTTTAAACTTAGGAG+TGG + chr8.1:65544382-65544401 None:intergenic 35.0%
TGCTATAGTATCACATCAGA+TGG - chr8.1:65545062-65545081 MS.gene056202:CDS 35.0%
TTATATGGCATTGCTGATAC+AGG - chr8.1:65544303-65544322 MS.gene056202:CDS 35.0%
TTTAAAGGGTCTTCAAATGG+TGG - chr8.1:65544900-65544919 MS.gene056202:CDS 35.0%
! ACCCCACCATTTAAGTTATA+TGG - chr8.1:65544288-65544307 MS.gene056202:CDS 35.0%
! ACGACAAGCAAACTCAATTT+TGG - chr8.1:65544759-65544778 MS.gene056202:CDS 35.0%
! TAATTTGGAATCAGCTGTGT+TGG - chr8.1:65544989-65545008 MS.gene056202:CDS 35.0%
! TTATTGTTTGTTGCCAAACC+CGG - chr8.1:65544725-65544744 MS.gene056202:CDS 35.0%
! TTATTTGTTGCTGTTGCAGA+TGG - chr8.1:65545149-65545168 MS.gene056202:CDS 35.0%
! TTGGTATAGTGGTGATTTTG+AGG + chr8.1:65544118-65544137 None:intergenic 35.0%
!! ACATTGGAAGCATTTAGTGT+TGG - chr8.1:65544861-65544880 MS.gene056202:CDS 35.0%
!! GGCAGTTTTGTAGAAATGAT+TGG + chr8.1:65544199-65544218 None:intergenic 35.0%
!! TTGCTTTTGCAGTTACTTCT+GGG - chr8.1:65545183-65545202 MS.gene056202:CDS 35.0%
!! TTTGCTTTTGCAGTTACTTC+TGG - chr8.1:65545182-65545201 MS.gene056202:CDS 35.0%
!!! GGTATTTGTTTTGTGTAGGT+TGG + chr8.1:65544137-65544156 None:intergenic 35.0%
!!! TTTTGTGTAGGTTGGTATAG+TGG + chr8.1:65544129-65544148 None:intergenic 35.0%
AAATGAAATAGGACGTCCAG+TGG + chr8.1:65544571-65544590 None:intergenic 40.0%
ACGTTGACAGTTATTCCAAC+TGG - chr8.1:65544957-65544976 MS.gene056202:CDS 40.0%
CAAAGGTTAAATTCTCGGGT+TGG + chr8.1:65545044-65545063 None:intergenic 40.0%
CCTAAAACTGTGATAGGATG+TGG - chr8.1:65544591-65544610 MS.gene056202:CDS 40.0%
CTGAGCTAAGTTTCCAAAGA+TGG + chr8.1:65545228-65545247 None:intergenic 40.0%
CTTAGCTCAGCAGAATTTGT+TGG - chr8.1:65545238-65545257 MS.gene056202:CDS 40.0%
GGTGATACGTCACAATCAAA+AGG - chr8.1:65544510-65544529 MS.gene056202:CDS 40.0%
TGAGATATTCACCATGATCG+GGG + chr8.1:65544251-65544270 None:intergenic 40.0%
TGATTGAGGTGTGTTTGTGA+GGG + chr8.1:65544220-65544239 None:intergenic 40.0%
TTACATAGGTCAGACGAACA+TGG + chr8.1:65544429-65544448 None:intergenic 40.0%
TTGATTGAGGTGTGTTTGTG+AGG + chr8.1:65544221-65544240 None:intergenic 40.0%
! CCACATCCTATCACAGTTTT+AGG + chr8.1:65544594-65544613 None:intergenic 40.0%
! TCTTCAAATGGTGGTGATGA+GGG - chr8.1:65544909-65544928 MS.gene056202:CDS 40.0%
AAGATGGAAAGGTCAGAACC+AGG + chr8.1:65545212-65545231 None:intergenic 45.0%
AGATGGAAAGGTCAGAACCA+GGG + chr8.1:65545211-65545230 None:intergenic 45.0%
CTTACATTAGAGTCCTCCAC+TGG - chr8.1:65544552-65544571 MS.gene056202:CDS 45.0%
CTTGTCGTGTTTGACTCAAC+CGG + chr8.1:65544747-65544766 None:intergenic 45.0%
GAAGTAAAAGAGGCTCAGCA+AGG + chr8.1:65544022-65544041 None:intergenic 45.0%
GGAATCAGCTGTGTTGGAAT+TGG - chr8.1:65544995-65545014 MS.gene056202:CDS 45.0%
TCAACTGTAATCCCCGATCA+TGG - chr8.1:65544237-65544256 MS.gene056202:CDS 45.0%
TCGGGGATTACAGTTGATTG+AGG + chr8.1:65544234-65544253 None:intergenic 45.0%
TTGTCGTGTTTGACTCAACC+GGG + chr8.1:65544746-65544765 None:intergenic 45.0%
! GATTGGCACGGTTTCTAGAA+CGG + chr8.1:65544182-65544201 None:intergenic 45.0%
! GGTTCTGACCTTTCCATCTT+TGG - chr8.1:65545212-65545231 MS.gene056202:CDS 45.0%
! GTCTTCAAATGGTGGTGATG+AGG - chr8.1:65544908-65544927 MS.gene056202:CDS 45.0%
! TCATTTGAAGGTGCAAGCTC+CGG - chr8.1:65544630-65544649 MS.gene056202:CDS 45.0%
!! GCTCAGCAGAATTTGTTGGT+TGG - chr8.1:65545242-65545261 MS.gene056202:CDS 45.0%
!! GTGATTTTGAGGAGTCACGA+TGG + chr8.1:65544107-65544126 None:intergenic 45.0%
!! GTGCGGCATTGACAATATGT+TGG + chr8.1:65544158-65544177 None:intergenic 45.0%
GTGTTTGACTCAACCGGGTT+TGG + chr8.1:65544741-65544760 None:intergenic 50.0%
TGAAATAGGACGTCCAGTGG+AGG + chr8.1:65544568-65544587 None:intergenic 50.0%
!! CAATTTTGGAGATGCCGCTG+TGG - chr8.1:65544773-65544792 MS.gene056202:CDS 50.0%
ACCTCCAAGGCCAACTACAC+CGG + chr8.1:65544652-65544671 None:intergenic 55.0%
GCAGTTACTTCTGGGTTCCC+TGG - chr8.1:65545191-65545210 MS.gene056202:CDS 55.0%
GGTTGACACTACACCATCCC+CGG + chr8.1:65544802-65544821 None:intergenic 55.0%
!! ACGGTTTCTAGAACGGCGTG+CGG + chr8.1:65544175-65544194 None:intergenic 55.0%
ACCATCCCCGGAAACCACAG+CGG + chr8.1:65544790-65544809 None:intergenic 60.0%
AGATGCCGCTGTGGTTTCCG+GGG - chr8.1:65544782-65544801 MS.gene056202:CDS 60.0%
GAGATGCCGCTGTGGTTTCC+GGG - chr8.1:65544781-65544800 MS.gene056202:CDS 60.0%
GGAGATGCCGCTGTGGTTTC+CGG - chr8.1:65544780-65544799 MS.gene056202:CDS 60.0%
GGTGCAAGCTCCGGTGTAGT+TGG - chr8.1:65544639-65544658 MS.gene056202:CDS 60.0%
! AGCTCCGGTGTAGTTGGCCT+TGG - chr8.1:65544645-65544664 MS.gene056202:CDS 60.0%
!! GGTGTAGTTGGCCTTGGAGG+TGG - chr8.1:65544651-65544670 MS.gene056202:CDS 60.0%
!! TCCGGTGTAGTTGGCCTTGG+AGG - chr8.1:65544648-65544667 MS.gene056202:CDS 60.0%
GAGACGCTGGGCCACCTCCA+AGG + chr8.1:65544665-65544684 None:intergenic 70.0%
GCCGCTGTGGTTTCCGGGGA+TGG - chr8.1:65544786-65544805 MS.gene056202:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 65544006 65545325 65544006 ID=MS.gene056202
chr8.1 mRNA 65544006 65545325 65544006 ID=MS.gene056202.t1;Parent=MS.gene056202
chr8.1 exon 65544006 65545325 65544006 ID=MS.gene056202.t1.exon1;Parent=MS.gene056202.t1
chr8.1 CDS 65544006 65545325 65544006 ID=cds.MS.gene056202.t1;Parent=MS.gene056202.t1
Gene Sequence

>MS.gene056202

ATGAATACATGTTCCTTGCTGAGCCTCTTTTACTTCTCCCTTTGTTTCATTATTTCTCTTTCTCATGCTTTAAACAACGGTTTTAGTGTTGAACTCATCCATCGTGACTCCTCAAAATCACCACTATACCAACCTACACAAAACAAATACCAACATATTGTCAATGCCGCACGCCGTTCTAGAAACCGTGCCAATCATTTCTACAAAACTGCCCTCACAAACACACCTCAATCAACTGTAATCCCCGATCATGGTGAATATCTCATGACATATTCAGTTGGTACCCCACCATTTAAGTTATATGGCATTGCTGATACAGGTAGTGACATTGTTTGGCTTCAATGCGAGCCTTGCAAAGAATGTTACAATCAAACCACTCCTAAGTTTAAACCATCAAAATCGTCAACTTACAAAAACATTCCATGTTCGTCTGACCTATGTAAATCTGTGCAACAAGGTGCCGATTGTAATGACAATTTTTTTTGTGAATATAGTATTTTTTATGGTGATACGTCACAATCAAAAGGAAATCTTAGTGTCGATACTCTTACATTAGAGTCCTCCACTGGACGTCCTATTTCATTTCCTAAAACTGTGATAGGATGTGGAACTGACAATACAATGTCATTTGAAGGTGCAAGCTCCGGTGTAGTTGGCCTTGGAGGTGGCCCAGCGTCTCTTATTACACAATTGGGATCATCAATTGATTCAAAATTCTCTTATTGTTTGTTGCCAAACCCGGTTGAGTCAAACACGACAAGCAAACTCAATTTTGGAGATGCCGCTGTGGTTTCCGGGGATGGTGTAGTGTCAACCCCTATAGTAAAAAAGGATCCTATAGTTTTCTACTATTTGACATTGGAAGCATTTAGTGTTGGTAACAAAAGAATAGAATTTAAAGGGTCTTCAAATGGTGGTGATGAGGGTAACATCATAATTGATTCTGGTACAACGTTGACAGTTATTCCAACTGGTGTTTATAATAATTTGGAATCAGCTGTGTTGGAATTGGTGAAACTGAAGCGTGTTGATGATCCAACCCGAGAATTTAACCTTTGCTATAGTATCACATCAGATGGATATGATTTTCCTATAATAACGGCACATTTTAAAGGTGCAGATGTTAAGTTGCATCCTATTAGTTTATTTGTTGCTGTTGCAGATGGCGTTGTTTGCTTTGCTTTTGCAGTTACTTCTGGGTTCCCTGGTTCTGACCTTTCCATCTTTGGAAACTTAGCTCAGCAGAATTTGTTGGTTGGTTATGACCTTCAACAAAAAATTGTGTCGTTTAAGCCTACTGATTGTAGCAAAGTGTTGTAG
Protein sequence

>MS.gene056202.t1

MNTCSLLSLFYFSLCFIISLSHALNNGFSVELIHRDSSKSPLYQPTQNKYQHIVNAARRSRNRANHFYKTALTNTPQSTVIPDHGEYLMTYSVGTPPFKLYGIADTGSDIVWLQCEPCKECYNQTTPKFKPSKSSTYKNIPCSSDLCKSVQQGADCNDNFFCEYSIFYGDTSQSKGNLSVDTLTLESSTGRPISFPKTVIGCGTDNTMSFEGASSGVVGLGGGPASLITQLGSSIDSKFSYCLLPNPVESNTTSKLNFGDAAVVSGDGVVSTPIVKKDPIVFYYLTLEAFSVGNKRIEFKGSSNGGDEGNIIIDSGTTLTVIPTGVYNNLESAVLELVKLKRVDDPTREFNLCYSITSDGYDFPIITAHFKGADVKLHPISLFVAVADGVVCFAFAVTSGFPGSDLSIFGNLAQQNLLVGYDLQQKIVSFKPTDCSKVL