AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056203

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056203.t1 MTR_8g038280 91.972 436 35 0 1 436 1 436 0.0 828
MS.gene056203.t1 MTR_8g096990 72.248 436 117 1 1 436 1 432 0.0 655
MS.gene056203.t1 MTR_5g080890 73.516 438 110 4 1 436 1 434 0.0 652
MS.gene056203.t1 MTR_5g080960 72.831 438 113 4 1 436 1 434 0.0 647
MS.gene056203.t1 MTR_8g038250 69.910 442 101 3 1 436 1 416 0.0 615
MS.gene056203.t1 MTR_5g044770 68.807 436 131 2 1 436 1 431 0.0 614
MS.gene056203.t1 MTR_6g090290 66.055 436 147 1 1 435 1 436 0.0 560
MS.gene056203.t1 MTR_8g038310 61.239 436 145 4 1 435 1 413 0.0 531
MS.gene056203.t1 MTR_4g109760 62.844 436 125 5 1 436 1 399 7.59e-174 492
MS.gene056203.t1 MTR_3g028120 52.844 422 187 6 23 436 22 439 4.02e-149 431
MS.gene056203.t1 MTR_5g038360 50.679 442 201 9 4 435 2 436 1.82e-141 411
MS.gene056203.t1 MTR_2g067830 54.258 411 176 8 32 435 1 406 7.18e-140 406
MS.gene056203.t1 MTR_6g088760 53.171 410 183 7 1 404 1 407 3.04e-137 404
MS.gene056203.t1 MTR_5g044570 48.198 444 184 11 4 435 2 411 1.12e-130 383
MS.gene056203.t1 MTR_2g105300 50.980 408 166 9 32 432 1 381 3.47e-125 368
MS.gene056203.t1 MTR_0024s0040 49.640 417 192 8 30 435 1 410 4.15e-125 369
MS.gene056203.t1 MTR_0036s0050 42.986 442 225 10 9 435 6 435 5.99e-110 331
MS.gene056203.t1 MTR_0036s0070 41.876 437 228 10 9 435 6 426 9.31e-108 325
MS.gene056203.t1 MTR_0036s0110 40.816 441 229 10 13 435 16 442 4.27e-99 303
MS.gene056203.t1 MTR_5g008070 41.546 414 219 9 27 434 29 425 1.07e-93 289
MS.gene056203.t1 MTR_5g008110 41.287 373 204 7 70 435 51 415 3.48e-91 282
MS.gene056203.t1 MTR_5g008150 40.779 385 212 8 59 435 36 412 1.28e-90 281
MS.gene056203.t1 MTR_2g059460 41.093 421 199 12 24 435 26 406 4.78e-90 279
MS.gene056203.t1 MTR_2g059490 44.011 359 188 8 86 436 58 411 8.83e-89 277
MS.gene056203.t1 MTR_3g097150 35.714 448 253 15 8 436 18 449 4.56e-75 241
MS.gene056203.t1 MTR_1g060750 36.609 407 215 11 40 435 2 376 3.06e-74 237
MS.gene056203.t1 MTR_4g045857 39.655 290 136 7 9 295 13 266 4.99e-58 192
MS.gene056203.t1 MTR_7g117430 34.411 433 240 17 24 433 73 484 3.87e-57 196
MS.gene056203.t1 MTR_5g008090 38.079 302 171 8 142 435 21 314 5.22e-57 191
MS.gene056203.t1 MTR_1g039270 31.973 441 248 18 22 433 52 469 5.05e-52 182
MS.gene056203.t1 MTR_1g115210 31.265 419 259 10 30 433 82 486 2.63e-51 180
MS.gene056203.t1 MTR_2g028190 33.030 439 258 14 20 433 24 451 4.33e-50 176
MS.gene056203.t1 MTR_1g111350 34.072 361 203 15 85 433 130 467 1.16e-46 167
MS.gene056203.t1 MTR_3g046300 32.732 388 237 11 68 436 79 461 7.02e-46 165
MS.gene056203.t1 MTR_3g053710 33.242 364 215 10 85 433 116 466 2.34e-44 161
MS.gene056203.t1 MTR_4g007740 32.754 403 224 16 55 434 100 478 8.25e-44 160
MS.gene056203.t1 MTR_6g088765 40.081 247 110 4 162 407 5 214 1.40e-42 150
MS.gene056203.t1 MTR_5g091130 30.404 421 262 13 38 436 145 556 2.44e-41 154
MS.gene056203.t1 MTR_6g085010 32.773 357 210 12 86 433 156 491 1.26e-40 151
MS.gene056203.t1 MTR_8g103880 30.056 356 223 12 87 433 117 455 4.90e-39 146
MS.gene056203.t1 MTR_2g070430 28.166 458 275 17 10 430 10 450 5.91e-37 140
MS.gene056203.t1 MTR_2g055840 29.058 382 232 13 78 433 5 373 2.54e-33 129
MS.gene056203.t1 MTR_7g105850 29.032 496 257 23 5 435 2 467 6.03e-31 124
MS.gene056203.t1 MTR_3g053980 31.481 378 222 13 73 433 128 485 2.46e-29 120
MS.gene056203.t1 MTR_8g094590 27.344 384 219 15 85 436 72 427 5.59e-29 118
MS.gene056203.t1 MTR_2g063110 26.891 476 266 18 28 433 45 508 8.10e-29 118
MS.gene056203.t1 MTR_3g065160 28.686 373 223 15 88 436 76 429 2.15e-28 116
MS.gene056203.t1 MTR_3g096930 25.743 404 239 15 65 436 63 437 1.90e-26 111
MS.gene056203.t1 MTR_3g068000 26.684 386 222 14 89 433 76 441 3.16e-26 110
MS.gene056203.t1 MTR_8g094590 28.571 364 201 15 85 421 72 403 3.47e-26 110
MS.gene056203.t1 MTR_7g080230 25.561 446 266 16 30 434 11 431 5.33e-26 110
MS.gene056203.t1 MTR_4g008110 48.333 120 35 1 158 277 45 137 1.15e-25 102
MS.gene056203.t1 MTR_1g078240 26.617 402 250 15 62 434 45 430 1.55e-25 108
MS.gene056203.t1 MTR_1g061940 26.998 463 280 22 7 434 6 445 6.07e-25 107
MS.gene056203.t1 MTR_4g095270 26.190 420 256 18 41 436 37 426 1.29e-24 106
MS.gene056203.t1 MTR_7g021510 28.424 387 224 21 82 434 67 434 1.40e-24 106
MS.gene056203.t1 MTR_4g095270 26.190 420 256 18 41 436 37 426 1.45e-24 107
MS.gene056203.t1 MTR_7g062360 26.425 386 227 15 89 436 62 428 1.48e-24 105
MS.gene056203.t1 MTR_1g078240 26.799 403 249 16 62 434 45 431 1.57e-24 105
MS.gene056203.t1 MTR_8g094590 31.679 262 146 10 85 337 72 309 1.94e-24 103
MS.gene056203.t1 MTR_3g006820 26.456 412 236 17 64 436 32 415 7.47e-24 103
MS.gene056203.t1 MTR_2g084550 28.571 378 227 18 85 434 76 438 1.57e-22 100
MS.gene056203.t1 MTR_8g039540 27.740 447 273 18 14 434 12 434 3.75e-22 98.6
MS.gene056203.t1 MTR_1g037630 27.586 435 261 19 24 434 25 429 4.44e-22 98.2
MS.gene056203.t1 MTR_7g102750 26.790 377 234 17 85 434 80 441 8.94e-22 97.8
MS.gene056203.t1 MTR_4g056420 27.851 377 230 18 85 434 76 437 1.02e-21 97.4
MS.gene056203.t1 MTR_5g016260 24.146 410 253 17 49 436 61 434 1.63e-21 97.4
MS.gene056203.t1 MTR_0945s0010 40.476 126 71 3 314 436 1 125 1.63e-21 91.3
MS.gene056203.t1 MTR_5g016260 24.146 410 253 17 49 436 61 434 2.44e-21 97.1
MS.gene056203.t1 MTR_5g077850 26.738 374 233 13 88 436 83 440 3.83e-21 95.5
MS.gene056203.t1 MTR_3g014290 28.646 384 227 16 69 434 63 417 6.99e-21 94.7
MS.gene056203.t1 MTR_1g029810 26.031 388 220 18 85 436 56 412 7.92e-21 94.4
MS.gene056203.t1 MTR_1g029810 26.031 388 220 18 85 436 56 412 8.89e-21 94.4
MS.gene056203.t1 MTR_2g103340 40.800 125 29 2 21 145 34 113 1.21e-20 89.7
MS.gene056203.t1 MTR_1g078240 27.381 336 207 13 62 376 45 364 1.30e-20 93.6
MS.gene056203.t1 MTR_8g075090 27.249 378 217 19 87 435 113 461 9.87e-20 92.0
MS.gene056203.t1 MTR_4g023650 24.631 406 246 17 66 436 42 422 8.09e-19 88.6
MS.gene056203.t1 MTR_5g022880 24.664 446 273 18 27 433 40 461 2.01e-18 87.8
MS.gene056203.t1 MTR_7g102750 27.011 348 213 16 85 406 80 412 2.01e-18 87.4
MS.gene056203.t1 MTR_5g061690 26.027 438 251 21 28 421 25 433 4.95e-18 86.7
MS.gene056203.t1 MTR_4g023630 23.267 404 254 14 66 436 42 422 2.10e-17 84.3
MS.gene056203.t1 MTR_5g012490 26.650 409 228 20 86 434 74 470 3.46e-17 84.0
MS.gene056203.t1 MTR_7g080230 25.352 355 214 14 110 434 28 361 1.33e-16 81.6
MS.gene056203.t1 MTR_3g087780 24.123 456 270 22 28 436 27 453 9.71e-16 79.7
MS.gene056203.t1 MTR_5g061690 27.458 295 180 15 138 421 23 294 3.59e-13 71.2
MS.gene056203.t1 MTR_5g077920 25.469 373 239 13 88 435 70 428 3.65e-13 71.2
MS.gene056203.t1 MTR_3g100500 28.693 352 212 16 85 420 96 424 8.53e-13 70.1
MS.gene056203.t1 MTR_1g072380 23.426 397 220 16 86 428 56 422 1.37e-11 66.6
MS.gene056203.t1 MTR_2g037710 28.037 214 124 9 227 433 15 205 2.53e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056203.t1 AT1G64830 49.034 414 204 4 25 436 23 431 5.01e-132 387
MS.gene056203.t1 AT5G33340 50.721 416 195 6 25 436 28 437 4.73e-127 375
MS.gene056203.t1 AT2G35615 44.144 444 223 7 12 434 5 444 8.93e-110 331
MS.gene056203.t1 AT1G31450 45.475 442 217 9 8 434 10 442 4.51e-107 324
MS.gene056203.t1 AT2G28010 38.710 434 210 11 9 436 11 394 2.08e-84 264
MS.gene056203.t1 AT2G28040 39.202 426 204 14 16 436 18 393 1.44e-81 257
MS.gene056203.t1 AT2G28040 39.663 416 196 14 26 436 19 384 4.08e-80 253
MS.gene056203.t1 AT2G28220 36.242 447 227 11 1 436 1 400 7.83e-77 245
MS.gene056203.t1 AT2G28030 41.444 374 188 10 69 436 42 390 2.79e-76 243
MS.gene056203.t1 AT2G03200 38.215 437 228 15 25 436 42 461 4.37e-76 244
MS.gene056203.t1 AT2G28225 35.491 448 229 13 1 436 1 400 6.27e-70 227
MS.gene056203.t1 AT1G01300 36.915 363 199 14 85 434 140 485 3.93e-54 187
MS.gene056203.t1 AT4G30030 35.356 379 210 16 70 433 61 419 5.62e-54 186
MS.gene056203.t1 AT3G61820 35.264 397 218 16 55 433 108 483 7.81e-54 187
MS.gene056203.t1 AT2G23945 28.817 465 271 16 9 436 10 451 5.33e-51 179
MS.gene056203.t1 AT3G18490 29.767 430 268 14 18 433 91 500 1.82e-50 178
MS.gene056203.t1 AT3G20015 29.091 440 266 16 20 433 51 470 2.92e-46 166
MS.gene056203.t1 AT5G10770 33.777 376 215 12 77 434 115 474 2.74e-45 164
MS.gene056203.t1 AT1G25510 33.613 357 210 11 85 433 146 483 1.32e-44 162
MS.gene056203.t1 AT4G30040 30.337 445 262 19 7 428 8 427 2.23e-42 155
MS.gene056203.t1 AT5G10760 32.000 425 245 16 29 433 64 464 5.06e-42 155
MS.gene056203.t1 AT3G25700 29.302 430 252 18 39 434 39 450 4.44e-40 149
MS.gene056203.t1 AT1G79720 32.880 368 206 12 87 433 135 482 5.50e-39 147
MS.gene056203.t1 AT3G12700 29.148 446 279 18 11 434 31 461 1.03e-38 145
MS.gene056203.t1 AT3G59080 31.367 373 230 14 85 436 168 535 1.04e-38 147
MS.gene056203.t1 AT2G42980 31.830 377 225 14 85 436 158 527 2.15e-38 146
MS.gene056203.t1 AT5G36260 29.396 381 216 15 85 434 76 434 1.31e-35 137
MS.gene056203.t1 AT2G39710 28.496 379 212 16 89 428 67 425 2.18e-30 122
MS.gene056203.t1 AT3G59080 30.435 368 204 13 85 436 168 499 4.58e-30 122
MS.gene056203.t1 AT3G54400 28.934 394 235 14 62 434 56 425 1.11e-28 117
MS.gene056203.t1 AT3G02740 28.198 383 217 17 85 434 83 440 6.28e-28 115
MS.gene056203.t1 AT3G02740 28.084 381 216 17 87 434 10 365 1.47e-27 114
MS.gene056203.t1 AT1G09750 27.979 386 235 14 72 433 82 448 3.95e-27 113
MS.gene056203.t1 AT4G12920 26.478 389 238 14 61 433 31 387 4.75e-27 112
MS.gene056203.t1 AT5G37540 26.613 372 233 13 88 433 81 438 5.89e-27 112
MS.gene056203.t1 AT1G66180 26.344 372 236 14 88 436 73 429 1.47e-26 111
MS.gene056203.t1 AT4G33490 26.166 386 222 15 85 436 58 414 1.77e-26 110
MS.gene056203.t1 AT1G65240 26.053 380 227 16 85 434 72 427 1.32e-25 108
MS.gene056203.t1 AT5G02190 27.441 379 216 18 96 433 82 442 4.00e-25 107
MS.gene056203.t1 AT2G36670 26.738 374 237 15 85 434 98 458 3.90e-24 105
MS.gene056203.t1 AT2G36670 26.613 372 236 15 87 434 34 392 1.23e-23 103
MS.gene056203.t1 AT2G36670 26.613 372 236 15 87 434 34 392 1.23e-23 103
MS.gene056203.t1 AT2G36670 26.613 372 236 15 87 434 105 463 1.32e-23 103
MS.gene056203.t1 AT5G43100 27.321 377 219 18 85 436 74 420 4.07e-23 101
MS.gene056203.t1 AT3G50050 26.161 409 251 17 55 436 55 439 5.64e-23 102
MS.gene056203.t1 AT1G05840 27.604 384 229 18 82 434 74 439 7.09e-23 101
MS.gene056203.t1 AT1G77480 24.675 385 230 16 85 436 65 422 8.02e-23 100
MS.gene056203.t1 AT5G43100 27.321 377 219 18 85 436 74 420 8.58e-23 101
MS.gene056203.t1 AT1G44130 25.926 405 238 16 68 436 24 402 1.35e-22 99.8
MS.gene056203.t1 AT1G77480 24.675 385 230 16 85 436 65 422 1.52e-22 100
MS.gene056203.t1 AT4G33490 28.664 307 173 13 85 374 55 332 3.95e-22 98.2
MS.gene056203.t1 AT2G17760 25.761 427 247 20 45 433 55 449 6.34e-22 98.6
MS.gene056203.t1 AT3G52500 28.960 404 210 19 85 434 88 468 1.10e-21 97.4
MS.gene056203.t1 AT3G51350 26.000 450 271 18 23 433 26 452 2.82e-21 96.7
MS.gene056203.t1 AT5G22850 27.513 378 231 18 85 434 79 441 4.66e-21 95.9
MS.gene056203.t1 AT1G08210 28.460 383 218 18 85 434 116 475 3.61e-19 90.1
MS.gene056203.t1 AT5G07030 26.573 429 259 18 20 421 43 442 4.31e-19 89.7
MS.gene056203.t1 AT1G08210 28.460 383 218 18 85 434 130 489 4.37e-19 90.1
MS.gene056203.t1 AT1G08210 28.460 383 218 18 85 434 82 441 4.51e-19 89.7
MS.gene056203.t1 AT1G49050 23.757 362 231 15 105 435 50 397 6.19e-19 89.0
MS.gene056203.t1 AT1G49050 24.619 394 239 17 80 435 197 570 1.35e-18 88.6
MS.gene056203.t1 AT1G08210 26.829 410 217 19 85 434 82 468 2.23e-15 78.6
MS.gene056203.t1 AT3G42550 22.427 379 223 12 74 434 65 390 3.29e-15 77.8
MS.gene056203.t1 AT3G25700 27.684 177 107 6 39 202 39 207 3.55e-15 77.0
MS.gene056203.t1 AT5G10080 25.104 482 271 27 11 435 7 455 2.43e-14 75.5
MS.gene056203.t1 AT1G49050 26.838 272 168 11 80 337 197 451 1.05e-13 73.2
MS.gene056203.t1 AT3G51360 25.405 370 220 18 92 433 104 445 1.83e-12 69.3
MS.gene056203.t1 AT3G51360 25.405 370 220 18 92 433 94 435 1.87e-12 69.3
MS.gene056203.t1 AT3G51360 25.405 370 220 18 92 433 10 351 1.94e-12 68.9

Find 91 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACGCCACCTTTGGAAATT+TGG 0.149363 8.1:-65538694 MS.gene056203:CDS
GTCAAATAGTAATAGGTTTC+AGG 0.170628 8.1:+65539059 None:intergenic
TGTGAATATTCTACTTATTA+TGG 0.199216 8.1:-65539422 MS.gene056203:CDS
ATACCGGTAGTGATATTATT+TGG 0.208017 8.1:-65539589 MS.gene056203:CDS
TTAAAATGTGTGGTGATTAT+AGG 0.215291 8.1:+65538804 None:intergenic
GGTACAATCAGTAGGTTTAA+AGG 0.233685 8.1:+65538623 None:intergenic
ACCCCACCATTTAAGTTATA+TGG 0.251212 8.1:-65539620 MS.gene056203:CDS
CCACATCCTATCACAATATT+AGG 0.259740 8.1:+65539320 None:intergenic
GCAATACCATATAACTTAAA+TGG 0.279077 8.1:+65539614 None:intergenic
GGAGATGCGGCTATGGTATC+TGG 0.323023 8.1:-65539119 MS.gene056203:CDS
GGAAATAAAAGAGTAGAAAT+TGG 0.326779 8.1:-65539017 MS.gene056203:CDS
TCCTATTAGCACATTTGTTC+CGG 0.333439 8.1:-65538760 MS.gene056203:CDS
TCATTTCCTAATATTGTGAT+AGG 0.341630 8.1:-65539326 MS.gene056203:CDS
TGCTGATGGTGTTGTTTGCT+TGG 0.342994 8.1:-65538736 MS.gene056203:CDS
TTCCAATGTCAAATAGTAAT+AGG 0.343339 8.1:+65539052 None:intergenic
ATGAGATACTCGCCTTTATC+AGG 0.348879 8.1:+65539659 None:intergenic
AACAAATGGAAATGAATAGA+AGG 0.352282 8.1:+65539879 None:intergenic
GGTAACATCATAATTGATTC+AGG 0.364855 8.1:-65538969 MS.gene056203:CDS
GGAATCTGCTGTGGTAGATT+TGG 0.372955 8.1:-65538904 MS.gene056203:CDS
CGGCTATGGTATCTGGCAAC+GGG 0.373663 8.1:-65539112 MS.gene056203:CDS
ATAGATTGACACAACTTTGA+TGG 0.374549 8.1:+65539476 None:intergenic
TCTCAAGATGACGCCACCTT+TGG 0.374689 8.1:-65538702 MS.gene056203:CDS
TCAACTGTAATACCTGATAA+AGG 0.391550 8.1:-65539671 MS.gene056203:CDS
AAACAATGGTGTTAAACAAT+AGG 0.394363 8.1:+65539187 None:intergenic
GAAATGATTGGCACGGTTGA+TGG 0.407103 8.1:+65539724 None:intergenic
TTTGGCTCAACAAAATTTGT+TGG 0.408329 8.1:-65538676 MS.gene056203:CDS
CCTAATATTGTGATAGGATG+TGG 0.415797 8.1:-65539320 MS.gene056203:CDS
GGCATAGTTGGCCTTGGAGG+TGG 0.421810 8.1:-65539260 MS.gene056203:CDS
CAAACAACACCATCAGCAAC+CGG 0.424779 8.1:+65538741 None:intergenic
GGCATAAGCTCTGGCATAGT+TGG 0.426354 8.1:-65539272 MS.gene056203:CDS
ACTCAATTGGGATCCTCAAC+TGG 0.427072 8.1:-65539221 MS.gene056203:CDS
AGCTCTGGCATAGTTGGCCT+TGG 0.428693 8.1:-65539266 MS.gene056203:CDS
AAAAGAAATAATGAAACAAA+TGG 0.430540 8.1:+65539865 None:intergenic
ACCGGAACAAATGTGCTAAT+AGG 0.436739 8.1:+65538759 None:intergenic
TCTGGCATAGTTGGCCTTGG+AGG 0.436740 8.1:-65539263 MS.gene056203:CDS
GATTAAACATAGGAGTGGTT+TGG 0.449157 8.1:+65539534 None:intergenic
TGGTGGGGTACCAACTGAAT+AGG 0.458426 8.1:+65539634 None:intergenic
TCAAATAGTAATAGGTTTCA+GGG 0.463391 8.1:+65539060 None:intergenic
GCAACGAGCAAGCTCAACTT+TGG 0.464327 8.1:-65539140 MS.gene056203:CDS
GCTCAACAAAATTTGTTGGT+TGG 0.467228 8.1:-65538672 MS.gene056203:CDS
TAGTGTTGATACTCTTACAT+TGG 0.467324 8.1:-65539372 MS.gene056203:CDS
TAGATTGACACAACTTTGAT+GGG 0.480149 8.1:+65539477 None:intergenic
AACAATATTATGTCTTATGA+AGG 0.482827 8.1:-65539293 MS.gene056203:CDS
CAACTTTGGAGATGCGGCTA+TGG 0.494629 8.1:-65539126 MS.gene056203:CDS
TCAGGTATTACAGTTGATTG+AGG 0.502820 8.1:+65539677 None:intergenic
TTGATTGAGGTATATTTGCA+AGG 0.505434 8.1:+65539690 None:intergenic
TACCATATAACTTAAATGGT+GGG 0.508960 8.1:+65539618 None:intergenic
AGTTGCAAATGGCGATAATG+AGG 0.526805 8.1:-65538991 MS.gene056203:CDS
CAAAGGCTTAATGTTTGAGT+AGG 0.527299 8.1:+65538858 None:intergenic
ACATTGGAAGCATTTAGTGT+TGG 0.529902 8.1:-65539038 MS.gene056203:CDS
GAAATTGGAGGAGTTGCAAA+TGG 0.537930 8.1:-65539002 MS.gene056203:CDS
TGAAACTGTAAGTCCATTGG+TGG 0.538274 8.1:+65539346 None:intergenic
TTGTAACTTGACGATTTGGA+CGG 0.538622 8.1:+65539512 None:intergenic
TCTTATGAAGGCATAAGCTC+TGG 0.539135 8.1:-65539281 MS.gene056203:CDS
ACATTTGTTCCGGTTGCTGA+TGG 0.540335 8.1:-65538750 MS.gene056203:CDS
GGAGAATTTACCACCAGTTG+AGG 0.544348 8.1:+65539208 None:intergenic
TGACACAACTTTGATGGGCA+AGG 0.547878 8.1:+65539482 None:intergenic
AATAAAAGAGTAGAAATTGG+AGG 0.549005 8.1:-65539014 MS.gene056203:CDS
TGCTATAGTGTCAAAGCTGA+GGG 0.551512 8.1:-65538837 MS.gene056203:CDS
TTGAGCCAAATTTCCAAAGG+TGG 0.552185 8.1:+65538689 None:intergenic
ACAAATGGAAATGAATAGAA+GGG 0.553290 8.1:+65539880 None:intergenic
GCGGCTATGGTATCTGGCAA+CGG 0.563430 8.1:-65539113 MS.gene056203:CDS
AAATGAAACTGTAAGTCCAT+TGG 0.564165 8.1:+65539343 None:intergenic
AAGCCAAATAATATCACTAC+CGG 0.565433 8.1:+65539586 None:intergenic
ATGGAAATGAATAGAAGGGT+AGG 0.565500 8.1:+65539884 None:intergenic
TTATATGGTATTGCTGATAC+CGG 0.565680 8.1:-65539605 MS.gene056203:CDS
TGATTGAGGTATATTTGCAA+GGG 0.566811 8.1:+65539691 None:intergenic
AACCTATTACTATTTGACAT+TGG 0.569683 8.1:-65539054 MS.gene056203:CDS
GGTGATAATTCACATTCAGA+AGG 0.574167 8.1:-65539401 MS.gene056203:CDS
CACACTTTGGTACAATCAGT+AGG 0.575657 8.1:+65538615 None:intergenic
ACCATATAACTTAAATGGTG+GGG 0.576438 8.1:+65539619 None:intergenic
CAGCTTTGACACTATAGCAA+AGG 0.579289 8.1:+65538841 None:intergenic
CTATAAAAGTGATCACACTT+TGG 0.586982 8.1:+65538602 None:intergenic
TGGTTGTAACATTGTTGACA+AGG 0.591014 8.1:+65539554 None:intergenic
ATACCATATAACTTAAATGG+TGG 0.591590 8.1:+65539617 None:intergenic
AAGATGCGGGTCCACCTCCA+AGG 0.595636 8.1:+65539249 None:intergenic
GATTTGGACGGATTAAACAT+AGG 0.598664 8.1:+65539524 None:intergenic
TTGTTGAGCCAAATTTCCAA+AGG 0.603204 8.1:+65538686 None:intergenic
TTGCTATAGTGTCAAAGCTG+AGG 0.607476 8.1:-65538838 MS.gene056203:CDS
GGACGGATTAAACATAGGAG+TGG 0.612846 8.1:+65539529 None:intergenic
AATTGAGTGATAAAAGATGC+GGG 0.617632 8.1:+65539236 None:intergenic
GTTGCAAATGGCGATAATGA+GGG 0.623062 8.1:-65538990 MS.gene056203:CDS
ATGTTTGTTACAGAAAACAA+TGG 0.624952 8.1:+65539173 None:intergenic
TATCTCATGACCTATTCAGT+TGG 0.628251 8.1:-65539644 MS.gene056203:CDS
CTTACATTGGAATCCACCAA+TGG 0.633185 8.1:-65539359 MS.gene056203:CDS
CAATTGAGTGATAAAAGATG+CGG 0.639675 8.1:+65539235 None:intergenic
ATCTGCACCTTTAAAATGTG+TGG 0.648432 8.1:+65538794 None:intergenic
CAAGCTCAACTTTGGAGATG+CGG 0.650651 8.1:-65539132 MS.gene056203:CDS
GGTGGCGTCATCTTGAGATG+AGG 0.659524 8.1:+65538707 None:intergenic
CAATTGGGATCCTCAACTGG+TGG 0.671748 8.1:-65539218 MS.gene056203:CDS
GGCTATGGTATCTGGCAACG+GGG 0.692106 8.1:-65539111 MS.gene056203:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGAAATAATGAAACAAA+TGG + chr8.1:65538654-65538673 None:intergenic 15.0%
!! AACAATATTATGTCTTATGA+AGG - chr8.1:65539223-65539242 MS.gene056203:CDS 20.0%
!! TGTGAATATTCTACTTATTA+TGG - chr8.1:65539094-65539113 MS.gene056203:CDS 20.0%
!!! AAAAGATGATTACTCTTTTT+TGG - chr8.1:65539591-65539610 MS.gene056203:CDS 20.0%
!!! TATTGATATTTGTTTTGAGT+AGG + chr8.1:65538749-65538768 None:intergenic 20.0%
! AACAAATGGAAATGAATAGA+AGG + chr8.1:65538640-65538659 None:intergenic 25.0%
! AACCTATTACTATTTGACAT+TGG - chr8.1:65539462-65539481 MS.gene056203:CDS 25.0%
! AATAAAAGAGTAGAAATTGG+AGG - chr8.1:65539502-65539521 MS.gene056203:CDS 25.0%
! ACAAATGGAAATGAATAGAA+GGG + chr8.1:65538639-65538658 None:intergenic 25.0%
! ATACCATATAACTTAAATGG+TGG + chr8.1:65538902-65538921 None:intergenic 25.0%
! GCAATACCATATAACTTAAA+TGG + chr8.1:65538905-65538924 None:intergenic 25.0%
! GGAAATAAAAGAGTAGAAAT+TGG - chr8.1:65539499-65539518 MS.gene056203:CDS 25.0%
! TACCATATAACTTAAATGGT+GGG + chr8.1:65538901-65538920 None:intergenic 25.0%
! TCAAATAGTAATAGGTTTCA+GGG + chr8.1:65539459-65539478 None:intergenic 25.0%
! TCATTTCCTAATATTGTGAT+AGG - chr8.1:65539190-65539209 MS.gene056203:CDS 25.0%
! TTAAAATGTGTGGTGATTAT+AGG + chr8.1:65539715-65539734 None:intergenic 25.0%
! TTCCAATGTCAAATAGTAAT+AGG + chr8.1:65539467-65539486 None:intergenic 25.0%
!! AAACAATGGTGTTAAACAAT+AGG + chr8.1:65539332-65539351 None:intergenic 25.0%
!! ATAATCACCACACATTTTAA+AGG - chr8.1:65539715-65539734 MS.gene056203:CDS 25.0%
!! ATGTTTGTTACAGAAAACAA+TGG + chr8.1:65539346-65539365 None:intergenic 25.0%
!! TTTTCTCATACTCAAAAGAA+TGG - chr8.1:65538671-65538690 MS.gene056203:CDS 25.0%
!!! ATAAGTTTTGTCATTACATG+AGG + chr8.1:65539074-65539093 None:intergenic 25.0%
AAATGAAACTGTAAGTCCAT+TGG + chr8.1:65539176-65539195 None:intergenic 30.0%
AAGCCAAATAATATCACTAC+CGG + chr8.1:65538933-65538952 None:intergenic 30.0%
AATTGAGTGATAAAAGATGC+GGG + chr8.1:65539283-65539302 None:intergenic 30.0%
ACCATATAACTTAAATGGTG+GGG + chr8.1:65538900-65538919 None:intergenic 30.0%
ATACCGGTAGTGATATTATT+TGG - chr8.1:65538927-65538946 MS.gene056203:CDS 30.0%
ATAGATTGACACAACTTTGA+TGG + chr8.1:65539043-65539062 None:intergenic 30.0%
CAATTGAGTGATAAAAGATG+CGG + chr8.1:65539284-65539303 None:intergenic 30.0%
GTCAAATAGTAATAGGTTTC+AGG + chr8.1:65539460-65539479 None:intergenic 30.0%
TAGATTGACACAACTTTGAT+GGG + chr8.1:65539042-65539061 None:intergenic 30.0%
TCAACTGTAATACCTGATAA+AGG - chr8.1:65538845-65538864 MS.gene056203:CDS 30.0%
TGATTGAGGTATATTTGCAA+GGG + chr8.1:65538828-65538847 None:intergenic 30.0%
TTATATGGTATTGCTGATAC+CGG - chr8.1:65538911-65538930 MS.gene056203:CDS 30.0%
TTGATTGAGGTATATTTGCA+AGG + chr8.1:65538829-65538848 None:intergenic 30.0%
! GCATCTTTTATCACTCAATT+GGG - chr8.1:65539283-65539302 MS.gene056203:CDS 30.0%
! GGAGTTTTTGTAGAAATGAT+TGG + chr8.1:65538807-65538826 None:intergenic 30.0%
! GGTAACATCATAATTGATTC+AGG - chr8.1:65539547-65539566 MS.gene056203:CDS 30.0%
! GTTTTTGTAACTTGACGATT+TGG + chr8.1:65539011-65539030 None:intergenic 30.0%
! TTTGGCTCAACAAAATTTGT+TGG - chr8.1:65539840-65539859 MS.gene056203:CDS 30.0%
! TTTTGTAGAAATGATTGGCA+CGG + chr8.1:65538802-65538821 None:intergenic 30.0%
!! TAGTGTTGATACTCTTACAT+TGG - chr8.1:65539144-65539163 MS.gene056203:CDS 30.0%
!! TTTTGAGTAGGTTTATAGAG+TGG + chr8.1:65538737-65538756 None:intergenic 30.0%
!!! TCAGGGTCTTTTTTCAATAT+AGG + chr8.1:65539442-65539461 None:intergenic 30.0%
ATGGAAATGAATAGAAGGGT+AGG + chr8.1:65538635-65538654 None:intergenic 35.0%
CAAAGGCTTAATGTTTGAGT+AGG + chr8.1:65539661-65539680 None:intergenic 35.0%
CCACATCCTATCACAATATT+AGG + chr8.1:65539199-65539218 None:intergenic 35.0%
CCTAATATTGTGATAGGATG+TGG - chr8.1:65539196-65539215 MS.gene056203:CDS 35.0%
GATTAAACATAGGAGTGGTT+TGG + chr8.1:65538985-65539004 None:intergenic 35.0%
GGTACAATCAGTAGGTTTAA+AGG + chr8.1:65539896-65539915 None:intergenic 35.0%
GGTGATAATTCACATTCAGA+AGG - chr8.1:65539115-65539134 MS.gene056203:CDS 35.0%
TATCTCATGACCTATTCAGT+TGG - chr8.1:65538872-65538891 MS.gene056203:CDS 35.0%
TCAGGTATTACAGTTGATTG+AGG + chr8.1:65538842-65538861 None:intergenic 35.0%
TCCTATTAGCACATTTGTTC+CGG - chr8.1:65539756-65539775 MS.gene056203:CDS 35.0%
TGGTTGTAACATTGTTGACA+AGG + chr8.1:65538965-65538984 None:intergenic 35.0%
TTGTTGAGCCAAATTTCCAA+AGG + chr8.1:65539833-65539852 None:intergenic 35.0%
! ACCCCACCATTTAAGTTATA+TGG - chr8.1:65538896-65538915 MS.gene056203:CDS 35.0%
! CGCATCTTTTATCACTCAAT+TGG - chr8.1:65539282-65539301 MS.gene056203:CDS 35.0%
! TTGTAACTTGACGATTTGGA+CGG + chr8.1:65539007-65539026 None:intergenic 35.0%
!! ACATTGGAAGCATTTAGTGT+TGG - chr8.1:65539478-65539497 MS.gene056203:CDS 35.0%
!! ATCTGCACCTTTAAAATGTG+TGG + chr8.1:65539725-65539744 None:intergenic 35.0%
!! GATTTGGACGGATTAAACAT+AGG + chr8.1:65538995-65539014 None:intergenic 35.0%
!! GCTCAACAAAATTTGTTGGT+TGG - chr8.1:65539844-65539863 MS.gene056203:CDS 35.0%
ACCGGAACAAATGTGCTAAT+AGG + chr8.1:65539760-65539779 None:intergenic 40.0%
AGTTGCAAATGGCGATAATG+AGG - chr8.1:65539525-65539544 MS.gene056203:CDS 40.0%
ATGAGATACTCGCCTTTATC+AGG + chr8.1:65538860-65538879 None:intergenic 40.0%
CACACTTTGGTACAATCAGT+AGG + chr8.1:65539904-65539923 None:intergenic 40.0%
CTTACATTGGAATCCACCAA+TGG - chr8.1:65539157-65539176 MS.gene056203:CDS 40.0%
GAAATTGGAGGAGTTGCAAA+TGG - chr8.1:65539514-65539533 MS.gene056203:CDS 40.0%
GTTGCAAATGGCGATAATGA+GGG - chr8.1:65539526-65539545 MS.gene056203:CDS 40.0%
TGAAACTGTAAGTCCATTGG+TGG + chr8.1:65539173-65539192 None:intergenic 40.0%
TGCTATAGTGTCAAAGCTGA+GGG - chr8.1:65539679-65539698 MS.gene056203:CDS 40.0%
TTGAGCCAAATTTCCAAAGG+TGG + chr8.1:65539830-65539849 None:intergenic 40.0%
TTGCTATAGTGTCAAAGCTG+AGG - chr8.1:65539678-65539697 MS.gene056203:CDS 40.0%
! CAGCTTTGACACTATAGCAA+AGG + chr8.1:65539678-65539697 None:intergenic 40.0%
! TCTTATGAAGGCATAAGCTC+TGG - chr8.1:65539235-65539254 MS.gene056203:CDS 40.0%
!! GTGGTGATTTTAAAGAGTCG+CGG + chr8.1:65538718-65538737 None:intergenic 40.0%
!!! CTCTTTTTTGGAATCTGCTG+TGG - chr8.1:65539603-65539622 MS.gene056203:CDS 40.0%
ACATTTGTTCCGGTTGCTGA+TGG - chr8.1:65539766-65539785 MS.gene056203:CDS 45.0%
ACTCAATTGGGATCCTCAAC+TGG - chr8.1:65539295-65539314 MS.gene056203:CDS 45.0%
CAAACAACACCATCAGCAAC+CGG + chr8.1:65539778-65539797 None:intergenic 45.0%
GAAATGATTGGCACGGTTGA+TGG + chr8.1:65538795-65538814 None:intergenic 45.0%
GGAATCTGCTGTGGTAGATT+TGG - chr8.1:65539612-65539631 MS.gene056203:CDS 45.0%
GGAGAATTTACCACCAGTTG+AGG + chr8.1:65539311-65539330 None:intergenic 45.0%
! CAAGCTCAACTTTGGAGATG+CGG - chr8.1:65539384-65539403 MS.gene056203:CDS 45.0%
! TGACACAACTTTGATGGGCA+AGG + chr8.1:65539037-65539056 None:intergenic 45.0%
! TGACGCCACCTTTGGAAATT+TGG - chr8.1:65539822-65539841 MS.gene056203:CDS 45.0%
!! GGACGGATTAAACATAGGAG+TGG + chr8.1:65538990-65539009 None:intergenic 45.0%
!! GTGATTTTAAAGAGTCGCGG+TGG + chr8.1:65538715-65538734 None:intergenic 45.0%
!! TGCTGATGGTGTTGTTTGCT+TGG - chr8.1:65539780-65539799 MS.gene056203:CDS 45.0%
CAATTGGGATCCTCAACTGG+TGG - chr8.1:65539298-65539317 MS.gene056203:CDS 50.0%
GCAACGAGCAAGCTCAACTT+TGG - chr8.1:65539376-65539395 MS.gene056203:CDS 50.0%
TCTCAAGATGACGCCACCTT+TGG - chr8.1:65539814-65539833 MS.gene056203:CDS 50.0%
! CAACTTTGGAGATGCGGCTA+TGG - chr8.1:65539390-65539409 MS.gene056203:CDS 50.0%
! GGCATAAGCTCTGGCATAGT+TGG - chr8.1:65539244-65539263 MS.gene056203:CDS 50.0%
! TGGTGGGGTACCAACTGAAT+AGG + chr8.1:65538885-65538904 None:intergenic 50.0%
CGGCTATGGTATCTGGCAAC+GGG - chr8.1:65539404-65539423 MS.gene056203:CDS 55.0%
GCGGCTATGGTATCTGGCAA+CGG - chr8.1:65539403-65539422 MS.gene056203:CDS 55.0%
GGAGATGCGGCTATGGTATC+TGG - chr8.1:65539397-65539416 MS.gene056203:CDS 55.0%
GGCTATGGTATCTGGCAACG+GGG - chr8.1:65539405-65539424 MS.gene056203:CDS 55.0%
GGTGGCGTCATCTTGAGATG+AGG + chr8.1:65539812-65539831 None:intergenic 55.0%
! AGCTCTGGCATAGTTGGCCT+TGG - chr8.1:65539250-65539269 MS.gene056203:CDS 55.0%
!! TCTGGCATAGTTGGCCTTGG+AGG - chr8.1:65539253-65539272 MS.gene056203:CDS 55.0%
AAGATGCGGGTCCACCTCCA+AGG + chr8.1:65539270-65539289 None:intergenic 60.0%
!! GGCATAGTTGGCCTTGGAGG+TGG - chr8.1:65539256-65539275 MS.gene056203:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 65538614 65539924 65538614 ID=MS.gene056203
chr8.1 mRNA 65538614 65539924 65538614 ID=MS.gene056203.t1;Parent=MS.gene056203
chr8.1 exon 65538614 65539924 65538614 ID=MS.gene056203.t1.exon1;Parent=MS.gene056203.t1
chr8.1 CDS 65538614 65539924 65538614 ID=cds.MS.gene056203.t1;Parent=MS.gene056203.t1
Gene Sequence

>MS.gene056203

ATGAGTAAACGTTCATTTCCTACCCTTCTATTCATTTCCATTTGTTTCATTATTTCTTTTTCTCATACTCAAAAGAATGGTTTTAGTGTTGAACTCATCCACCGCGACTCTTTAAAATCACCACTCTATAAACCTACTCAAAACAAATATCAATATTTTGTCAATGCTGCACGTCGTTCCATCAACCGTGCCAATCATTTCTACAAAAACTCCCTTGCAAATATACCTCAATCAACTGTAATACCTGATAAAGGCGAGTATCTCATGACCTATTCAGTTGGTACCCCACCATTTAAGTTATATGGTATTGCTGATACCGGTAGTGATATTATTTGGCTTCAATGTGAGCCTTGTCAACAATGTTACAACCAAACCACTCCTATGTTTAATCCGTCCAAATCGTCAAGTTACAAAAACATTCCTTGCCCATCAAAGTTGTGTCAATCTATGAAAGATACCTCATGTAATGACAAAACTTATTGTGAATATTCTACTTATTATGGTGATAATTCACATTCAGAAGGAGATCTTAGTGTTGATACTCTTACATTGGAATCCACCAATGGACTTACAGTTTCATTTCCTAATATTGTGATAGGATGTGGAACTAACAATATTATGTCTTATGAAGGCATAAGCTCTGGCATAGTTGGCCTTGGAGGTGGACCCGCATCTTTTATCACTCAATTGGGATCCTCAACTGGTGGTAAATTCTCCTATTGTTTAACACCATTGTTTTCTGTAACAAACATTGAGTCAAACGCAACGAGCAAGCTCAACTTTGGAGATGCGGCTATGGTATCTGGCAACGGGGTTGTGACAACTCCTATATTGAAAAAAGACCCTGAAACCTATTACTATTTGACATTGGAAGCATTTAGTGTTGGAAATAAAAGAGTAGAAATTGGAGGAGTTGCAAATGGCGATAATGAGGGTAACATCATAATTGATTCAGGTACAACATTGACAACTTTAACAAAAGATGATTACTCTTTTTTGGAATCTGCTGTGGTAGATTTGGTGACGTTAGAACGTGTTGACGATCCTACTCAAACATTAAGCCTTTGCTATAGTGTCAAAGCTGAGGGATATGATTTTCCTATAATCACCACACATTTTAAAGGTGCAGATGTCGATTTGCATCCTATTAGCACATTTGTTCCGGTTGCTGATGGTGTTGTTTGCTTGGCTTTTGCCTCATCTCAAGATGACGCCACCTTTGGAAATTTGGCTCAACAAAATTTGTTGGTTGGTTATGATCTTCAGCAAAAAATTGTGTCCTTTAAACCTACTGATTGTACCAAAGTGTGA
Protein sequence

>MS.gene056203.t1

MSKRSFPTLLFISICFIISFSHTQKNGFSVELIHRDSLKSPLYKPTQNKYQYFVNAARRSINRANHFYKNSLANIPQSTVIPDKGEYLMTYSVGTPPFKLYGIADTGSDIIWLQCEPCQQCYNQTTPMFNPSKSSSYKNIPCPSKLCQSMKDTSCNDKTYCEYSTYYGDNSHSEGDLSVDTLTLESTNGLTVSFPNIVIGCGTNNIMSYEGISSGIVGLGGGPASFITQLGSSTGGKFSYCLTPLFSVTNIESNATSKLNFGDAAMVSGNGVVTTPILKKDPETYYYLTLEAFSVGNKRVEIGGVANGDNEGNIIIDSGTTLTTLTKDDYSFLESAVVDLVTLERVDDPTQTLSLCYSVKAEGYDFPIITTHFKGADVDLHPISTFVPVADGVVCLAFASSQDDATFGNLAQQNLLVGYDLQQKIVSFKPTDCTKV