Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056413.t1 | XP_003606841.1 | 78.3 | 198 | 43 | 0 | 1 | 198 | 1 | 198 | 3.10E-74 | 288.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056413.t1 | Q9FJG2 | 31.1 | 106 | 72 | 1 | 4 | 108 | 13 | 118 | 7.8e-10 | 65.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056413.t1 | G7JEY0 | 78.3 | 198 | 43 | 0 | 1 | 198 | 1 | 198 | 2.2e-74 | 288.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene056413.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056413 | MS.gene056615 | 0.892904 | 1.03E-74 | -1.69E-46 |
| MS.gene056413 | MS.gene28308 | 0.841272 | 5.25E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056413.t1 | MTR_4g068310 | 89.899 | 198 | 20 | 0 | 1 | 198 | 1 | 198 | 2.15e-128 | 359 |
| MS.gene056413.t1 | MTR_4g068300 | 57.789 | 199 | 63 | 6 | 1 | 180 | 1 | 197 | 4.22e-62 | 194 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056413.t1 | AT5G18090 | 28.671 | 143 | 97 | 4 | 4 | 144 | 11 | 150 | 9.87e-13 | 65.9 |
| MS.gene056413.t1 | AT5G18000 | 31.132 | 106 | 72 | 1 | 4 | 108 | 13 | 118 | 2.11e-12 | 64.7 |
| MS.gene056413.t1 | AT3G17010 | 28.402 | 169 | 108 | 5 | 4 | 164 | 14 | 177 | 1.92e-11 | 62.0 |
Find 41 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTTTCTCTTGGCCACAAT+TGG | 0.131130 | 4.1:+33761138 | None:intergenic |
| ATGTTCAAGGTGGAAATTTA+TGG | 0.176554 | 4.1:-33761216 | MS.gene056413:CDS |
| ACCGAAGATTGTTATGGATT+TGG | 0.260147 | 4.1:-33761421 | MS.gene056413:CDS |
| GAAGAAAGCATCAATTATTC+TGG | 0.262336 | 4.1:-33761385 | MS.gene056413:CDS |
| TGGATTTGGGTGAAGAGTTT+TGG | 0.266007 | 4.1:-33761407 | MS.gene056413:CDS |
| TTCTTTGATGATAGCGAAAA+AGG | 0.271206 | 4.1:+33761956 | None:intergenic |
| CCGAAGATTGTTATGGATTT+GGG | 0.289825 | 4.1:-33761420 | MS.gene056413:CDS |
| AGTGGGACTTGAAGAGTTCT+TGG | 0.304248 | 4.1:-33761262 | MS.gene056413:CDS |
| TTTCCAACGCATACTCTCTT+TGG | 0.308501 | 4.1:+33761018 | None:intergenic |
| CCCTTCTTTGTCACAATTGT+TGG | 0.332631 | 4.1:+33760976 | None:intergenic |
| TCTTTCTCTTGGCCACAATT+GGG | 0.346000 | 4.1:+33761139 | None:intergenic |
| GTGGAAATTTATGGTAAGAA+TGG | 0.371009 | 4.1:-33761207 | MS.gene056413:CDS |
| TTTGTCTTAGCCACAATTGT+TGG | 0.398096 | 4.1:+33761060 | None:intergenic |
| TGGTCTACCCCTCTTTCTCT+TGG | 0.402146 | 4.1:+33761128 | None:intergenic |
| AGTACCACCGAAGATTGTTA+TGG | 0.409721 | 4.1:-33761427 | MS.gene056413:CDS |
| TGGAAATTTATGGTAAGAAT+GGG | 0.420098 | 4.1:-33761206 | MS.gene056413:CDS |
| CTTTCTCTTGGCCACAATTG+GGG | 0.448018 | 4.1:+33761140 | None:intergenic |
| CCCAAATCCATAACAATCTT+CGG | 0.452160 | 4.1:+33761420 | None:intergenic |
| GACATTTATCTGCGAAACAT+TGG | 0.475455 | 4.1:-33761312 | MS.gene056413:CDS |
| ACCAACAATTGTGACAAAGA+AGG | 0.484661 | 4.1:-33760977 | MS.gene056413:CDS |
| TCTACTAAAATAACAGAAAG+AGG | 0.490912 | 4.1:+33760931 | None:intergenic |
| CAATTGTGGCCAAGAGAAAG+AGG | 0.520539 | 4.1:-33761137 | MS.gene056413:CDS |
| CCAACAATTGTGACAAAGAA+GGG | 0.536191 | 4.1:-33760976 | MS.gene056413:CDS |
| TATTGGAGATAACATGTTCA+AGG | 0.538893 | 4.1:-33761229 | MS.gene056413:CDS |
| AAAATAACAGAAAGAGGAGC+AGG | 0.548640 | 4.1:+33760937 | None:intergenic |
| TTTATCTGCGAAACATTGGT+TGG | 0.556011 | 4.1:-33761308 | MS.gene056413:CDS |
| AGTTGTTGCAGCCCCAATTG+TGG | 0.571592 | 4.1:-33761151 | MS.gene056413:CDS |
| AATTGTGGCTAAGACAAAGA+TGG | 0.585870 | 4.1:-33761055 | MS.gene056413:CDS |
| AGAAGCTGCACCAACAATTG+TGG | 0.589864 | 4.1:-33761070 | MS.gene056413:CDS |
| CAAATGAATTACCATGCACT+TGG | 0.596053 | 4.1:+33761923 | None:intergenic |
| TATGCTCTTCGAGTGAGAAG+TGG | 0.604662 | 4.1:-33761362 | MS.gene056413:CDS |
| AGGCTTCAACACCAAGTGCA+TGG | 0.605306 | 4.1:-33761934 | MS.gene056413:intron |
| ATGCTCTTCGAGTGAGAAGT+GGG | 0.605893 | 4.1:-33761361 | MS.gene056413:CDS |
| TTTCTGAAAGACAACTCAGT+GGG | 0.610997 | 4.1:-33761279 | MS.gene056413:CDS |
| AATCCAAAGAGAGTATGCGT+TGG | 0.611249 | 4.1:-33761021 | MS.gene056413:CDS |
| AATTGTGGCCAAGAGAAAGA+GGG | 0.618437 | 4.1:-33761136 | MS.gene056413:CDS |
| TGGAGATAACATGTTCAAGG+TGG | 0.674338 | 4.1:-33761226 | MS.gene056413:CDS |
| CGTTGGAAAACAAAACGCAG+AGG | 0.680803 | 4.1:-33761004 | MS.gene056413:CDS |
| ATTGTGGCCAAGAGAAAGAG+GGG | 0.688040 | 4.1:-33761135 | MS.gene056413:CDS |
| AAATCCATAACAATCTTCGG+TGG | 0.712870 | 4.1:+33761423 | None:intergenic |
| GTTTCTGAAAGACAACTCAG+TGG | 0.730659 | 4.1:-33761280 | MS.gene056413:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACAAAAAAATAAAAAACA+TGG | + | chr4.1:33761447-33761466 | None:intergenic | 10.0% |
| !!! | CAATAATATGTTTACTTTTT+TGG | - | chr4.1:33761294-33761313 | MS.gene056413:CDS | 15.0% |
| !! | ATTATACATATATATGAACC+CGG | - | chr4.1:33761212-33761231 | MS.gene056413:CDS | 20.0% |
| !! | TAGAAAAATGTTATTAAGCT+AGG | - | chr4.1:33761134-33761153 | MS.gene056413:CDS | 20.0% |
| !! | TATATATGTATAATCTCTGT+TGG | + | chr4.1:33761206-33761225 | None:intergenic | 20.0% |
| !!! | GAAGTTAAAATTTTGAAGAA+AGG | - | chr4.1:33761572-33761591 | MS.gene056413:intron | 20.0% |
| ! | ATATTATTGAGCTTGATATC+TGG | + | chr4.1:33761283-33761302 | None:intergenic | 25.0% |
| ! | TCTACTAAAATAACAGAAAG+AGG | + | chr4.1:33761983-33762002 | None:intergenic | 25.0% |
| ! | TGGAAATTTATGGTAAGAAT+GGG | - | chr4.1:33761705-33761724 | MS.gene056413:intron | 25.0% |
| !!! | TACTGAATGAGTTTTAAACT+TGG | + | chr4.1:33761171-33761190 | None:intergenic | 25.0% |
| !!! | TTCTTGGTTTTCACATATAT+TGG | - | chr4.1:33761665-33761684 | MS.gene056413:intron | 25.0% |
| ATGTTCAAGGTGGAAATTTA+TGG | - | chr4.1:33761695-33761714 | MS.gene056413:intron | 30.0% | |
| GTGGAAATTTATGGTAAGAA+TGG | - | chr4.1:33761704-33761723 | MS.gene056413:intron | 30.0% | |
| TATTGGAGATAACATGTTCA+AGG | - | chr4.1:33761682-33761701 | MS.gene056413:intron | 30.0% | |
| ! | TTCTTTGATGATAGCGAAAA+AGG | + | chr4.1:33760958-33760977 | None:intergenic | 30.0% |
| ! | TTTTTCGCTATCATCAAAGA+AGG | - | chr4.1:33760957-33760976 | MS.gene056413:CDS | 30.0% |
| !! | GAAGAAAGCATCAATTATTC+TGG | - | chr4.1:33761526-33761545 | MS.gene056413:intron | 30.0% |
| AAAAAATCCTGCTGATGTCA+AGG | - | chr4.1:33761808-33761827 | MS.gene056413:intron | 35.0% | |
| AAAATAACAGAAAGAGGAGC+AGG | + | chr4.1:33761977-33761996 | None:intergenic | 35.0% | |
| AAATCCATAACAATCTTCGG+TGG | + | chr4.1:33761491-33761510 | None:intergenic | 35.0% | |
| AATTGTGGCTAAGACAAAGA+TGG | - | chr4.1:33761856-33761875 | MS.gene056413:intron | 35.0% | |
| ACCAACAATTGTGACAAAGA+AGG | - | chr4.1:33761934-33761953 | MS.gene056413:intron | 35.0% | |
| ACCGAAGATTGTTATGGATT+TGG | - | chr4.1:33761490-33761509 | MS.gene056413:intron | 35.0% | |
| CAAATGAATTACCATGCACT+TGG | + | chr4.1:33760991-33761010 | None:intergenic | 35.0% | |
| CCAACAATTGTGACAAAGAA+GGG | - | chr4.1:33761935-33761954 | MS.gene056413:CDS | 35.0% | |
| CCCAAATCCATAACAATCTT+CGG | + | chr4.1:33761494-33761513 | None:intergenic | 35.0% | |
| CCGAAGATTGTTATGGATTT+GGG | - | chr4.1:33761491-33761510 | MS.gene056413:intron | 35.0% | |
| GACATTTATCTGCGAAACAT+TGG | - | chr4.1:33761599-33761618 | MS.gene056413:intron | 35.0% | |
| GAGAAATACCTGTCTTTCTT+TGG | - | chr4.1:33761064-33761083 | MS.gene056413:CDS | 35.0% | |
| TTTATCTGCGAAACATTGGT+TGG | - | chr4.1:33761603-33761622 | MS.gene056413:intron | 35.0% | |
| TTTGTCTTAGCCACAATTGT+TGG | + | chr4.1:33761854-33761873 | None:intergenic | 35.0% | |
| ! | TTGATGATAGCGAAAAAGGA+TGG | + | chr4.1:33760954-33760973 | None:intergenic | 35.0% |
| ! | TTTCTGAAAGACAACTCAGT+GGG | - | chr4.1:33761632-33761651 | MS.gene056413:intron | 35.0% |
| !!! | ACATCAGCAGGATTTTTTCT+TGG | + | chr4.1:33761806-33761825 | None:intergenic | 35.0% |
| !!! | TCTTGCTTTTTCTTGAAGCA+TGG | + | chr4.1:33761740-33761759 | None:intergenic | 35.0% |
| AATTGTGGCCAAGAGAAAGA+GGG | - | chr4.1:33761775-33761794 | MS.gene056413:intron | 40.0% | |
| AGTACCACCGAAGATTGTTA+TGG | - | chr4.1:33761484-33761503 | MS.gene056413:intron | 40.0% | |
| ATCAGCATCCAAAGAAAGAC+AGG | + | chr4.1:33761075-33761094 | None:intergenic | 40.0% | |
| CCCTTCTTTGTCACAATTGT+TGG | + | chr4.1:33761938-33761957 | None:intergenic | 40.0% | |
| TCTTTCTCTTGGCCACAATT+GGG | + | chr4.1:33761775-33761794 | None:intergenic | 40.0% | |
| TGGAGATAACATGTTCAAGG+TGG | - | chr4.1:33761685-33761704 | MS.gene056413:intron | 40.0% | |
| TTTCCAACGCATACTCTCTT+TGG | + | chr4.1:33761896-33761915 | None:intergenic | 40.0% | |
| ! | AATCCAAAGAGAGTATGCGT+TGG | - | chr4.1:33761890-33761909 | MS.gene056413:intron | 40.0% |
| ! | GTTTCTGAAAGACAACTCAG+TGG | - | chr4.1:33761631-33761650 | MS.gene056413:intron | 40.0% |
| ! | TAATCTCTGTTGGTGAGCTA+AGG | + | chr4.1:33761196-33761215 | None:intergenic | 40.0% |
| ! | TGGATTTGGGTGAAGAGTTT+TGG | - | chr4.1:33761504-33761523 | MS.gene056413:intron | 40.0% |
| ! | TTTTCTTCCTTGACATCAGC+AGG | + | chr4.1:33761818-33761837 | None:intergenic | 40.0% |
| AGAAGCTGCACCAACAATTG+TGG | - | chr4.1:33761841-33761860 | MS.gene056413:intron | 45.0% | |
| AGTGGGACTTGAAGAGTTCT+TGG | - | chr4.1:33761649-33761668 | MS.gene056413:intron | 45.0% | |
| ATATGAACCCGGATTCCCTA+CGG | - | chr4.1:33761223-33761242 | MS.gene056413:CDS | 45.0% | |
| ATGCTCTTCGAGTGAGAAGT+GGG | - | chr4.1:33761550-33761569 | MS.gene056413:intron | 45.0% | |
| ATTGTGGCCAAGAGAAAGAG+GGG | - | chr4.1:33761776-33761795 | MS.gene056413:intron | 45.0% | |
| CAATTGTGGCCAAGAGAAAG+AGG | - | chr4.1:33761774-33761793 | MS.gene056413:intron | 45.0% | |
| CGTTGGAAAACAAAACGCAG+AGG | - | chr4.1:33761907-33761926 | MS.gene056413:intron | 45.0% | |
| CTCTTTCTCTTGGCCACAAT+TGG | + | chr4.1:33761776-33761795 | None:intergenic | 45.0% | |
| CTTTCTCTTGGCCACAATTG+GGG | + | chr4.1:33761774-33761793 | None:intergenic | 45.0% | |
| GATCATCCAATCGAGATGGA+CGG | + | chr4.1:33761321-33761340 | None:intergenic | 45.0% | |
| GCTTGCATTGAATGACCGTA+GGG | + | chr4.1:33761241-33761260 | None:intergenic | 45.0% | |
| GTCATTCAATGCAAGCAGAC+AGG | - | chr4.1:33761245-33761264 | MS.gene056413:CDS | 45.0% | |
| TATGCTCTTCGAGTGAGAAG+TGG | - | chr4.1:33761549-33761568 | MS.gene056413:intron | 45.0% | |
| TCGTGATCATCCAATCGAGA+TGG | + | chr4.1:33761325-33761344 | None:intergenic | 45.0% | |
| TGCTTGCATTGAATGACCGT+AGG | + | chr4.1:33761242-33761261 | None:intergenic | 45.0% | |
| TTGAATGACCGTAGGGAATC+CGG | + | chr4.1:33761234-33761253 | None:intergenic | 45.0% | |
| AGGCTTCAACACCAAGTGCA+TGG | - | chr4.1:33760977-33760996 | MS.gene056413:CDS | 50.0% | |
| AGTTGTTGCAGCCCCAATTG+TGG | - | chr4.1:33761760-33761779 | MS.gene056413:intron | 50.0% | |
| GTGAATGCTTGCAGTCTGCA+GGG | - | chr4.1:33761360-33761379 | MS.gene056413:CDS | 50.0% | |
| TGAATGACCGTAGGGAATCC+GGG | + | chr4.1:33761233-33761252 | None:intergenic | 50.0% | |
| TGGTCTACCCCTCTTTCTCT+TGG | + | chr4.1:33761786-33761805 | None:intergenic | 50.0% | |
| TTTGGACCGTCCATCTCGAT+TGG | - | chr4.1:33761312-33761331 | MS.gene056413:CDS | 50.0% | |
| CAGTCTGCAGGGAATTCGAC+TGG | - | chr4.1:33761371-33761390 | MS.gene056413:CDS | 55.0% | |
| CGTGAATGCTTGCAGTCTGC+AGG | - | chr4.1:33761359-33761378 | MS.gene056413:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 33760927 | 33762006 | 33760927 | ID=MS.gene056413 |
| chr4.1 | mRNA | 33760927 | 33762006 | 33760927 | ID=MS.gene056413.t1;Parent=MS.gene056413 |
| chr4.1 | exon | 33761935 | 33762006 | 33761935 | ID=MS.gene056413.t1.exon1;Parent=MS.gene056413.t1 |
| chr4.1 | CDS | 33761935 | 33762006 | 33761935 | ID=cds.MS.gene056413.t1;Parent=MS.gene056413.t1 |
| chr4.1 | exon | 33760927 | 33761451 | 33760927 | ID=MS.gene056413.t1.exon2;Parent=MS.gene056413.t1 |
| chr4.1 | CDS | 33760927 | 33761451 | 33760927 | ID=cds.MS.gene056413.t1;Parent=MS.gene056413.t1 |
| Gene Sequence |
| Protein sequence |
