Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056615.t1 | XP_003606841.1 | 78.3 | 198 | 43 | 0 | 1 | 198 | 1 | 198 | 3.10E-74 | 288.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056615.t1 | Q9FJG2 | 31.1 | 106 | 72 | 1 | 4 | 108 | 13 | 118 | 7.8e-10 | 65.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056615.t1 | G7JEY0 | 78.3 | 198 | 43 | 0 | 1 | 198 | 1 | 198 | 2.2e-74 | 288.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene056615.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056413 | MS.gene056615 | 0.892904 | 1.03E-74 | -1.69E-46 |
| MS.gene056615 | MS.gene28308 | 0.933873 | 9.65E-96 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056615.t1 | MTR_4g068310 | 89.899 | 198 | 20 | 0 | 1 | 198 | 1 | 198 | 2.15e-128 | 359 |
| MS.gene056615.t1 | MTR_4g068300 | 57.789 | 199 | 63 | 6 | 1 | 180 | 1 | 197 | 4.22e-62 | 194 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056615.t1 | AT5G18090 | 28.671 | 143 | 97 | 4 | 4 | 144 | 11 | 150 | 9.87e-13 | 65.9 |
| MS.gene056615.t1 | AT5G18000 | 31.132 | 106 | 72 | 1 | 4 | 108 | 13 | 118 | 2.11e-12 | 64.7 |
| MS.gene056615.t1 | AT3G17010 | 28.402 | 169 | 108 | 5 | 4 | 164 | 14 | 177 | 1.92e-11 | 62.0 |
Find 41 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTTTCTCTTGGCCACAAT+TGG | 0.131130 | 4.3:+36702026 | None:intergenic |
| ATGTTCAAGGTGGAAATTTA+TGG | 0.176554 | 4.3:-36702104 | MS.gene056615:CDS |
| ACCGAAGATTGTTATGGATT+TGG | 0.260147 | 4.3:-36702309 | MS.gene056615:CDS |
| GAAGAAAGCATCAATTATTC+TGG | 0.262336 | 4.3:-36702273 | MS.gene056615:CDS |
| TGGATTTGGGTGAAGAGTTT+TGG | 0.266007 | 4.3:-36702295 | MS.gene056615:CDS |
| TTCTTTGATGATAGCGAAAA+AGG | 0.271206 | 4.3:+36702836 | None:intergenic |
| CCGAAGATTGTTATGGATTT+GGG | 0.289825 | 4.3:-36702308 | MS.gene056615:CDS |
| AGTGGGACTTGAAGAGTTCT+TGG | 0.304248 | 4.3:-36702150 | MS.gene056615:CDS |
| TTTCCAACGCATACTCTCTT+TGG | 0.308501 | 4.3:+36701906 | None:intergenic |
| CCCTTCTTTGTCACAATTGT+TGG | 0.332631 | 4.3:+36701864 | None:intergenic |
| TCTTTCTCTTGGCCACAATT+GGG | 0.346000 | 4.3:+36702027 | None:intergenic |
| GTGGAAATTTATGGTAAGAA+TGG | 0.371009 | 4.3:-36702095 | MS.gene056615:CDS |
| TTTGTCTTAGCCACAATTGT+TGG | 0.398096 | 4.3:+36701948 | None:intergenic |
| TGGTCTACCCCTCTTTCTCT+TGG | 0.402146 | 4.3:+36702016 | None:intergenic |
| AGTACCACCGAAGATTGTTA+TGG | 0.409721 | 4.3:-36702315 | MS.gene056615:CDS |
| TGGAAATTTATGGTAAGAAT+GGG | 0.420098 | 4.3:-36702094 | MS.gene056615:CDS |
| CTTTCTCTTGGCCACAATTG+GGG | 0.448018 | 4.3:+36702028 | None:intergenic |
| CCCAAATCCATAACAATCTT+CGG | 0.452160 | 4.3:+36702308 | None:intergenic |
| GACATTTATCTGCGAAACAT+TGG | 0.475455 | 4.3:-36702200 | MS.gene056615:CDS |
| ACCAACAATTGTGACAAAGA+AGG | 0.484661 | 4.3:-36701865 | MS.gene056615:CDS |
| TCTACTAAAATAACAGAAAG+AGG | 0.490912 | 4.3:+36701819 | None:intergenic |
| CAATTGTGGCCAAGAGAAAG+AGG | 0.520539 | 4.3:-36702025 | MS.gene056615:CDS |
| CCAACAATTGTGACAAAGAA+GGG | 0.536191 | 4.3:-36701864 | MS.gene056615:CDS |
| TATTGGAGATAACATGTTCA+AGG | 0.538893 | 4.3:-36702117 | MS.gene056615:CDS |
| AAAATAACAGAAAGAGGAGC+AGG | 0.548640 | 4.3:+36701825 | None:intergenic |
| TTTATCTGCGAAACATTGGT+TGG | 0.556011 | 4.3:-36702196 | MS.gene056615:CDS |
| AGTTGTTGCAGCCCCAATTG+TGG | 0.571592 | 4.3:-36702039 | MS.gene056615:CDS |
| AATTGTGGCTAAGACAAAGA+TGG | 0.585870 | 4.3:-36701943 | MS.gene056615:CDS |
| AGAAGCTGCACCAACAATTG+TGG | 0.589864 | 4.3:-36701958 | MS.gene056615:CDS |
| CAAATGAATTACCATGCACT+TGG | 0.596053 | 4.3:+36702803 | None:intergenic |
| TATGCTCTTCGAGTGAGAAG+TGG | 0.604662 | 4.3:-36702250 | MS.gene056615:CDS |
| AGGCTTCAACACCAAGTGCA+TGG | 0.605306 | 4.3:-36702814 | MS.gene056615:intron |
| ATGCTCTTCGAGTGAGAAGT+GGG | 0.605893 | 4.3:-36702249 | MS.gene056615:CDS |
| TTTCTGAAAGACAACTCAGT+GGG | 0.610997 | 4.3:-36702167 | MS.gene056615:CDS |
| AATCCAAAGAGAGTATGCGT+TGG | 0.611249 | 4.3:-36701909 | MS.gene056615:CDS |
| AATTGTGGCCAAGAGAAAGA+GGG | 0.618437 | 4.3:-36702024 | MS.gene056615:CDS |
| TGGAGATAACATGTTCAAGG+TGG | 0.674338 | 4.3:-36702114 | MS.gene056615:CDS |
| CGTTGGAAAACAAAACGCAG+AGG | 0.680803 | 4.3:-36701892 | MS.gene056615:CDS |
| ATTGTGGCCAAGAGAAAGAG+GGG | 0.688040 | 4.3:-36702023 | MS.gene056615:CDS |
| AAATCCATAACAATCTTCGG+TGG | 0.712870 | 4.3:+36702311 | None:intergenic |
| GTTTCTGAAAGACAACTCAG+TGG | 0.730659 | 4.3:-36702168 | MS.gene056615:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATGACAAAATAAAAAACA+TGG | + | chr4.3:36702335-36702354 | None:intergenic | 15.0% |
| !!! | CAATAATATGTTTACTTTTT+TGG | - | chr4.3:36702182-36702201 | MS.gene056615:CDS | 15.0% |
| !! | ATTATACATATATATGAACC+CGG | - | chr4.3:36702100-36702119 | MS.gene056615:CDS | 20.0% |
| !! | TAGAAAAATGTTATTAAGCT+AGG | - | chr4.3:36702022-36702041 | MS.gene056615:CDS | 20.0% |
| !! | TATATATGTATAATCTCTGT+TGG | + | chr4.3:36702094-36702113 | None:intergenic | 20.0% |
| !!! | GAAGTTAAAATTTTGAAGAA+AGG | - | chr4.3:36702452-36702471 | MS.gene056615:intron | 20.0% |
| ! | ATATTATTGAGCTTGATATC+TGG | + | chr4.3:36702171-36702190 | None:intergenic | 25.0% |
| ! | TCTACTAAAATAACAGAAAG+AGG | + | chr4.3:36702863-36702882 | None:intergenic | 25.0% |
| ! | TGGAAATTTATGGTAAGAAT+GGG | - | chr4.3:36702585-36702604 | MS.gene056615:intron | 25.0% |
| !!! | TACTGAATGAGTTTTAAACT+TGG | + | chr4.3:36702059-36702078 | None:intergenic | 25.0% |
| !!! | TTCTTGGTTTTCACATATAT+TGG | - | chr4.3:36702545-36702564 | MS.gene056615:intron | 25.0% |
| ATGTTCAAGGTGGAAATTTA+TGG | - | chr4.3:36702575-36702594 | MS.gene056615:intron | 30.0% | |
| GTGGAAATTTATGGTAAGAA+TGG | - | chr4.3:36702584-36702603 | MS.gene056615:intron | 30.0% | |
| TATTGGAGATAACATGTTCA+AGG | - | chr4.3:36702562-36702581 | MS.gene056615:intron | 30.0% | |
| ! | TTCTTTGATGATAGCGAAAA+AGG | + | chr4.3:36701846-36701865 | None:intergenic | 30.0% |
| ! | TTTTTCGCTATCATCAAAGA+AGG | - | chr4.3:36701845-36701864 | MS.gene056615:CDS | 30.0% |
| !! | GAAGAAAGCATCAATTATTC+TGG | - | chr4.3:36702406-36702425 | MS.gene056615:intron | 30.0% |
| AAAAAATCCTGCTGATGTCA+AGG | - | chr4.3:36702688-36702707 | MS.gene056615:intron | 35.0% | |
| AAAATAACAGAAAGAGGAGC+AGG | + | chr4.3:36702857-36702876 | None:intergenic | 35.0% | |
| AAATCCATAACAATCTTCGG+TGG | + | chr4.3:36702371-36702390 | None:intergenic | 35.0% | |
| AATTGTGGCTAAGACAAAGA+TGG | - | chr4.3:36702736-36702755 | MS.gene056615:intron | 35.0% | |
| ACCAACAATTGTGACAAAGA+AGG | - | chr4.3:36702814-36702833 | MS.gene056615:intron | 35.0% | |
| ACCGAAGATTGTTATGGATT+TGG | - | chr4.3:36702370-36702389 | MS.gene056615:intron | 35.0% | |
| CAAATGAATTACCATGCACT+TGG | + | chr4.3:36701879-36701898 | None:intergenic | 35.0% | |
| CCAACAATTGTGACAAAGAA+GGG | - | chr4.3:36702815-36702834 | MS.gene056615:CDS | 35.0% | |
| CCCAAATCCATAACAATCTT+CGG | + | chr4.3:36702374-36702393 | None:intergenic | 35.0% | |
| CCGAAGATTGTTATGGATTT+GGG | - | chr4.3:36702371-36702390 | MS.gene056615:intron | 35.0% | |
| GACATTTATCTGCGAAACAT+TGG | - | chr4.3:36702479-36702498 | MS.gene056615:intron | 35.0% | |
| GAGAAATACCTGTCTTTCTT+TGG | - | chr4.3:36701952-36701971 | MS.gene056615:CDS | 35.0% | |
| TTTATCTGCGAAACATTGGT+TGG | - | chr4.3:36702483-36702502 | MS.gene056615:intron | 35.0% | |
| TTTGTCTTAGCCACAATTGT+TGG | + | chr4.3:36702734-36702753 | None:intergenic | 35.0% | |
| ! | TTGATGATAGCGAAAAAGGA+TGG | + | chr4.3:36701842-36701861 | None:intergenic | 35.0% |
| ! | TTTCTGAAAGACAACTCAGT+GGG | - | chr4.3:36702512-36702531 | MS.gene056615:intron | 35.0% |
| !!! | ACATCAGCAGGATTTTTTCT+TGG | + | chr4.3:36702686-36702705 | None:intergenic | 35.0% |
| !!! | TCTTGCTTTTTCTTGAAGCA+TGG | + | chr4.3:36702620-36702639 | None:intergenic | 35.0% |
| AATTGTGGCCAAGAGAAAGA+GGG | - | chr4.3:36702655-36702674 | MS.gene056615:intron | 40.0% | |
| AGTACCACCGAAGATTGTTA+TGG | - | chr4.3:36702364-36702383 | MS.gene056615:intron | 40.0% | |
| ATCAGCATCCAAAGAAAGAC+AGG | + | chr4.3:36701963-36701982 | None:intergenic | 40.0% | |
| CCCTTCTTTGTCACAATTGT+TGG | + | chr4.3:36702818-36702837 | None:intergenic | 40.0% | |
| TCTTTCTCTTGGCCACAATT+GGG | + | chr4.3:36702655-36702674 | None:intergenic | 40.0% | |
| TGGAGATAACATGTTCAAGG+TGG | - | chr4.3:36702565-36702584 | MS.gene056615:intron | 40.0% | |
| TTTCCAACGCATACTCTCTT+TGG | + | chr4.3:36702776-36702795 | None:intergenic | 40.0% | |
| ! | AATCCAAAGAGAGTATGCGT+TGG | - | chr4.3:36702770-36702789 | MS.gene056615:intron | 40.0% |
| ! | GTTTCTGAAAGACAACTCAG+TGG | - | chr4.3:36702511-36702530 | MS.gene056615:intron | 40.0% |
| ! | TAATCTCTGTTGGTGAGCTA+AGG | + | chr4.3:36702084-36702103 | None:intergenic | 40.0% |
| ! | TGGATTTGGGTGAAGAGTTT+TGG | - | chr4.3:36702384-36702403 | MS.gene056615:intron | 40.0% |
| ! | TTTTCTTCCTTGACATCAGC+AGG | + | chr4.3:36702698-36702717 | None:intergenic | 40.0% |
| AGAAGCTGCACCAACAATTG+TGG | - | chr4.3:36702721-36702740 | MS.gene056615:intron | 45.0% | |
| AGTGGGACTTGAAGAGTTCT+TGG | - | chr4.3:36702529-36702548 | MS.gene056615:intron | 45.0% | |
| ATATGAACCCGGATTCCCTA+CGG | - | chr4.3:36702111-36702130 | MS.gene056615:CDS | 45.0% | |
| ATGCTCTTCGAGTGAGAAGT+GGG | - | chr4.3:36702430-36702449 | MS.gene056615:intron | 45.0% | |
| ATTGTGGCCAAGAGAAAGAG+GGG | - | chr4.3:36702656-36702675 | MS.gene056615:intron | 45.0% | |
| CAATTGTGGCCAAGAGAAAG+AGG | - | chr4.3:36702654-36702673 | MS.gene056615:intron | 45.0% | |
| CGTTGGAAAACAAAACGCAG+AGG | - | chr4.3:36702787-36702806 | MS.gene056615:intron | 45.0% | |
| CTCTTTCTCTTGGCCACAAT+TGG | + | chr4.3:36702656-36702675 | None:intergenic | 45.0% | |
| CTTTCTCTTGGCCACAATTG+GGG | + | chr4.3:36702654-36702673 | None:intergenic | 45.0% | |
| GATCATCCAATCGAGATGGA+CGG | + | chr4.3:36702209-36702228 | None:intergenic | 45.0% | |
| GCTTGCATTGAATGACCGTA+GGG | + | chr4.3:36702129-36702148 | None:intergenic | 45.0% | |
| GTCATTCAATGCAAGCAGAC+AGG | - | chr4.3:36702133-36702152 | MS.gene056615:CDS | 45.0% | |
| TATGCTCTTCGAGTGAGAAG+TGG | - | chr4.3:36702429-36702448 | MS.gene056615:intron | 45.0% | |
| TCGTGATCATCCAATCGAGA+TGG | + | chr4.3:36702213-36702232 | None:intergenic | 45.0% | |
| TGCTTGCATTGAATGACCGT+AGG | + | chr4.3:36702130-36702149 | None:intergenic | 45.0% | |
| TTGAATGACCGTAGGGAATC+CGG | + | chr4.3:36702122-36702141 | None:intergenic | 45.0% | |
| AGGCTTCAACACCAAGTGCA+TGG | - | chr4.3:36701865-36701884 | MS.gene056615:CDS | 50.0% | |
| AGTTGTTGCAGCCCCAATTG+TGG | - | chr4.3:36702640-36702659 | MS.gene056615:intron | 50.0% | |
| GTGAATGCTTGCAGTCTGCA+GGG | - | chr4.3:36702248-36702267 | MS.gene056615:CDS | 50.0% | |
| TGAATGACCGTAGGGAATCC+GGG | + | chr4.3:36702121-36702140 | None:intergenic | 50.0% | |
| TGGTCTACCCCTCTTTCTCT+TGG | + | chr4.3:36702666-36702685 | None:intergenic | 50.0% | |
| TTTGGACCGTCCATCTCGAT+TGG | - | chr4.3:36702200-36702219 | MS.gene056615:CDS | 50.0% | |
| CAGTCTGCAGGGAATTCGAC+TGG | - | chr4.3:36702259-36702278 | MS.gene056615:CDS | 55.0% | |
| CGTGAATGCTTGCAGTCTGC+AGG | - | chr4.3:36702247-36702266 | MS.gene056615:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 36701815 | 36702886 | 36701815 | ID=MS.gene056615 |
| chr4.3 | mRNA | 36701815 | 36702886 | 36701815 | ID=MS.gene056615.t1;Parent=MS.gene056615 |
| chr4.3 | exon | 36702815 | 36702886 | 36702815 | ID=MS.gene056615.t1.exon1;Parent=MS.gene056615.t1 |
| chr4.3 | CDS | 36702815 | 36702886 | 36702815 | ID=cds.MS.gene056615.t1;Parent=MS.gene056615.t1 |
| chr4.3 | exon | 36701815 | 36702339 | 36701815 | ID=MS.gene056615.t1.exon2;Parent=MS.gene056615.t1 |
| chr4.3 | CDS | 36701815 | 36702339 | 36701815 | ID=cds.MS.gene056615.t1;Parent=MS.gene056615.t1 |
| Gene Sequence |
| Protein sequence |
