Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056535.t1 | XP_013446284.1 | 96.5 | 173 | 6 | 0 | 1 | 173 | 1 | 173 | 1.30E-63 | 252.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056535.t1 | P42735 | 53.8 | 173 | 66 | 3 | 1 | 173 | 4 | 162 | 1.9e-15 | 84.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056535.t1 | I3S7C4 | 96.5 | 173 | 6 | 0 | 1 | 173 | 1 | 173 | 9.2e-64 | 252.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056535 | MS.gene83424 | 0.808445 | 3.09E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056535.t1 | MTR_8g071180 | 96.532 | 173 | 6 | 0 | 1 | 173 | 1 | 173 | 1.26e-122 | 343 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056535.t1 | AT4G19070 | 53.179 | 173 | 67 | 3 | 1 | 173 | 4 | 162 | 1.04e-55 | 174 |
| MS.gene056535.t1 | AT4G19070 | 53.179 | 173 | 67 | 3 | 1 | 173 | 1 | 159 | 1.35e-55 | 173 |
Find 47 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTTCTTGTGGTCATCTTTC+TGG | 0.048652 | 8.3:+31302655 | MS.gene056535:CDS |
| GTTCTAATAATGATTATAAA+AGG | 0.238226 | 8.3:+31301542 | None:intergenic |
| CCCTCGTAAAGGTATTATTT+TGG | 0.247253 | 8.3:+31302882 | MS.gene056535:CDS |
| GGTATCACTCTTGTTGGTTT+TGG | 0.272786 | 8.3:+31301725 | MS.gene056535:CDS |
| ATCTTTGCAGCTCCTTATAA+TGG | 0.287147 | 8.3:+31301770 | MS.gene056535:CDS |
| TGTGGTGTTGGATGGGGTCC+TGG | 0.292274 | 8.3:+31301644 | MS.gene056535:CDS |
| GTTGGATGGGGTCCTGGATT+TGG | 0.298350 | 8.3:+31301650 | MS.gene056535:CDS |
| CACAAGCCTCAGGGGATGTT+TGG | 0.315024 | 8.3:+31302680 | MS.gene056535:CDS |
| AACCCTGTTCATCCAACTTA+TGG | 0.315421 | 8.3:+31301590 | MS.gene056535:CDS |
| CTTTACGAGGGCTGAATAAC+TGG | 0.320207 | 8.3:-31302870 | None:intergenic |
| GCTCCATAAGTTGGATGAAC+AGG | 0.342945 | 8.3:-31301593 | None:intergenic |
| CGTTGGTTGTGGTGTTGGAT+GGG | 0.354052 | 8.3:+31301637 | MS.gene056535:CDS |
| AAAAGAGGTATACCATTATA+AGG | 0.354158 | 8.3:-31301782 | None:intergenic |
| TTCGGCGTTGGTTGTGGTGT+TGG | 0.368268 | 8.3:+31301632 | MS.gene056535:CDS |
| AATGTTGGTATCACTCTTGT+TGG | 0.368632 | 8.3:+31301719 | MS.gene056535:CDS |
| GCGTTGGTTGTGGTGTTGGA+TGG | 0.382518 | 8.3:+31301636 | MS.gene056535:CDS |
| CCTGAAGTTGTTGGCTATGT+TGG | 0.398231 | 8.3:+31301674 | MS.gene056535:CDS |
| CCAACATAGCCAACAACTTC+AGG | 0.400009 | 8.3:-31301674 | None:intergenic |
| CTTTCTGGTCCACAAGCCTC+AGG | 0.405935 | 8.3:+31302670 | MS.gene056535:CDS |
| ATGGGTTTCGGCGTTGGTTG+TGG | 0.411499 | 8.3:+31301626 | MS.gene056535:CDS |
| GTTGGAGCTGGTTGTGGCGT+TGG | 0.416943 | 8.3:+31301692 | MS.gene056535:CDS |
| CATGAAGAACAGCTTGCCTT+TGG | 0.419512 | 8.3:+31302807 | MS.gene056535:CDS |
| TTGGAGCTGGTTGTGGCGTT+GGG | 0.434951 | 8.3:+31301693 | MS.gene056535:CDS |
| GGATTTGGTCCTGAAGTTGT+TGG | 0.437390 | 8.3:+31301665 | MS.gene056535:CDS |
| TATGGAATGGGTTTCGGCGT+TGG | 0.445305 | 8.3:+31301620 | MS.gene056535:CDS |
| CCATAAAATGCTCCATAAGT+TGG | 0.451487 | 8.3:-31301602 | None:intergenic |
| GTTGTTGGCTATGTTGGAGC+TGG | 0.461109 | 8.3:+31301680 | MS.gene056535:CDS |
| GTTGGTTGTGGTGTTGGATG+GGG | 0.464756 | 8.3:+31301638 | MS.gene056535:CDS |
| GGCTATGTTGGAGCTGGTTG+TGG | 0.466785 | 8.3:+31301686 | MS.gene056535:CDS |
| TGTTTAGAAGATATGAAAGA+TGG | 0.483303 | 8.3:+31301567 | MS.gene056535:CDS |
| TCTGACTTTCAAAGAGAAGC+TGG | 0.521844 | 8.3:+31302724 | MS.gene056535:CDS |
| TGTGGCGTTGGGTTCAATGT+TGG | 0.535275 | 8.3:+31301704 | MS.gene056535:CDS |
| CAGTTATTCAGCCCTCGTAA+AGG | 0.540196 | 8.3:+31302871 | MS.gene056535:CDS |
| GCTGCAAAGATAACATTAGC+AGG | 0.540585 | 8.3:-31301758 | None:intergenic |
| ACAACTTCAGGACCAAATCC+AGG | 0.557849 | 8.3:-31301662 | None:intergenic |
| CAAACATCCCCTGAGGCTTG+TGG | 0.576117 | 8.3:-31302679 | None:intergenic |
| CAAAAGTATTTATCAATCAA+CGG | 0.579321 | 8.3:+31302769 | MS.gene056535:CDS |
| TTTCTGGTCCACAAGCCTCA+GGG | 0.581201 | 8.3:+31302671 | MS.gene056535:CDS |
| TTGAAGTTAGCACGTTCTTG+TGG | 0.587715 | 8.3:+31302643 | MS.gene056535:CDS |
| CTCCATAAGTTGGATGAACA+GGG | 0.608622 | 8.3:-31301592 | None:intergenic |
| CCAAAATAATACCTTTACGA+GGG | 0.610802 | 8.3:-31302882 | None:intergenic |
| TTCTGGTCCACAAGCCTCAG+GGG | 0.613524 | 8.3:+31302672 | MS.gene056535:CDS |
| CTTTGAAAGTCAGACACGGA+AGG | 0.618621 | 8.3:-31302715 | None:intergenic |
| TTCTCTTTGAAAGTCAGACA+CGG | 0.624465 | 8.3:-31302719 | None:intergenic |
| ACCAAAATAATACCTTTACG+AGG | 0.635161 | 8.3:-31302883 | None:intergenic |
| CAGGCAGAAGTAGTAACCAA+AGG | 0.639806 | 8.3:-31302823 | None:intergenic |
| TCTAATCCAAACATCCCCTG+AGG | 0.750357 | 8.3:-31302686 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATATGATATTTTTAAGTAA+AGG | + | chr8.3:31301892-31301911 | MS.gene056535:intron | 10.0% |
| !!! | TACATTCTTAAATTATTTTC+AGG | - | chr8.3:31301961-31301980 | None:intergenic | 15.0% |
| !! | AGTTCAATTTAGTATCAAAT+TGG | + | chr8.3:31302430-31302449 | MS.gene056535:intron | 20.0% |
| !! | ATACACAGTTAACAAAAAAA+TGG | + | chr8.3:31302267-31302286 | MS.gene056535:intron | 20.0% |
| !! | GTTCAATTTAGTATCAAATT+GGG | + | chr8.3:31302431-31302450 | MS.gene056535:intron | 20.0% |
| !! | TGGTTTATGATAGAATATTA+TGG | + | chr8.3:31302165-31302184 | MS.gene056535:intron | 20.0% |
| !! | TTAGAATTATCATTGTGAAA+CGG | + | chr8.3:31302330-31302349 | MS.gene056535:intron | 20.0% |
| !!! | CAAAAGTATTTATCAATCAA+CGG | + | chr8.3:31302769-31302788 | MS.gene056535:CDS | 20.0% |
| !!! | TTTTGTTAACTGTGTATATT+CGG | - | chr8.3:31302264-31302283 | None:intergenic | 20.0% |
| ! | AGATATAGAGATTAATGAGT+TGG | + | chr8.3:31302133-31302152 | MS.gene056535:intron | 25.0% |
| ! | ATTGATGCAAATATTATGTG+TGG | + | chr8.3:31302495-31302514 | MS.gene056535:intron | 25.0% |
| ! | TAGAATTATCATTGTGAAAC+GGG | + | chr8.3:31302331-31302350 | MS.gene056535:intron | 25.0% |
| ! | TGTTTAGAAGATATGAAAGA+TGG | + | chr8.3:31301567-31301586 | MS.gene056535:CDS | 25.0% |
| !! | AAAAGAGGTATACCATTATA+AGG | - | chr8.3:31301785-31301804 | None:intergenic | 25.0% |
| !! | AAATGCTTGTATACTTTTAC+AGG | - | chr8.3:31302845-31302864 | None:intergenic | 25.0% |
| !! | CTATTTTCTGAATCGTAAAT+CGG | + | chr8.3:31301849-31301868 | MS.gene056535:intron | 25.0% |
| !! | TCTCTAATGTGTATCTATTT+TGG | - | chr8.3:31302397-31302416 | None:intergenic | 25.0% |
| !!! | GTGAAAAATTCTCTTGTTTT+CGG | + | chr8.3:31302746-31302765 | MS.gene056535:CDS | 25.0% |
| AAAGAATCATTACGTAAACG+TGG | - | chr8.3:31302302-31302321 | None:intergenic | 30.0% | |
| ACCAAAATAATACCTTTACG+AGG | - | chr8.3:31302886-31302905 | None:intergenic | 30.0% | |
| ACGTTTACGTAATGATTCTT+TGG | + | chr8.3:31302301-31302320 | MS.gene056535:intron | 30.0% | |
| CAAAAGAGCTGGATTAAAAT+AGG | - | chr8.3:31302605-31302624 | None:intergenic | 30.0% | |
| CCAAAATAATACCTTTACGA+GGG | - | chr8.3:31302885-31302904 | None:intergenic | 30.0% | |
| TAGATAAGATGAAGGATAGT+CGG | + | chr8.3:31302057-31302076 | MS.gene056535:intron | 30.0% | |
| ! | TAGAGATTAATGAGTTGGAT+CGG | + | chr8.3:31302138-31302157 | MS.gene056535:intron | 30.0% |
| !! | TATGGAGCATTTTATGGAAT+GGG | + | chr8.3:31301608-31301627 | MS.gene056535:CDS | 30.0% |
| !! | TTATGGAGCATTTTATGGAA+TGG | + | chr8.3:31301607-31301626 | MS.gene056535:CDS | 30.0% |
| !!! | CTATTTTAATCCAGCTCTTT+TGG | + | chr8.3:31302603-31302622 | MS.gene056535:intron | 30.0% |
| AAAATCCTTATCCCACTAAG+TGG | - | chr8.3:31302216-31302235 | None:intergenic | 35.0% | |
| AAATCCTTATCCCACTAAGT+GGG | - | chr8.3:31302215-31302234 | None:intergenic | 35.0% | |
| AACGAGAGTAGATAAGATGA+AGG | + | chr8.3:31302049-31302068 | MS.gene056535:intron | 35.0% | |
| AATGTTGGTATCACTCTTGT+TGG | + | chr8.3:31301719-31301738 | MS.gene056535:CDS | 35.0% | |
| ATCTTTGCAGCTCCTTATAA+TGG | + | chr8.3:31301770-31301789 | MS.gene056535:CDS | 35.0% | |
| CCATAAAATGCTCCATAAGT+TGG | - | chr8.3:31301605-31301624 | None:intergenic | 35.0% | |
| CTCGTTACTACAAAATCAAC+AGG | - | chr8.3:31302035-31302054 | None:intergenic | 35.0% | |
| GCGTAACAATCAACAAAAAG+AGG | - | chr8.3:31301800-31301819 | None:intergenic | 35.0% | |
| TATAAGAGAAACCCTGTAGA+TGG | + | chr8.3:31301988-31302007 | MS.gene056535:intron | 35.0% | |
| ! | ACTTAGTGGGATAAGGATTT+TGG | + | chr8.3:31302215-31302234 | MS.gene056535:intron | 35.0% |
| ! | TAATGAGTTGGATCGGAATA+TGG | + | chr8.3:31302145-31302164 | MS.gene056535:intron | 35.0% |
| ! | TTCTCTTTGAAAGTCAGACA+CGG | - | chr8.3:31302722-31302741 | None:intergenic | 35.0% |
| !! | CCAACTTATGGAGCATTTTA+TGG | + | chr8.3:31301602-31301621 | MS.gene056535:CDS | 35.0% |
| !! | GCATTTTATGGAATGGGTTT+CGG | + | chr8.3:31301614-31301633 | MS.gene056535:CDS | 35.0% |
| !!! | ACTCTTGTTGGTTTTGGAAT+TGG | + | chr8.3:31301731-31301750 | MS.gene056535:CDS | 35.0% |
| !!! | CCCTCGTAAAGGTATTATTT+TGG | + | chr8.3:31302882-31302901 | MS.gene056535:CDS | 35.0% |
| AACCCTGTTCATCCAACTTA+TGG | + | chr8.3:31301590-31301609 | MS.gene056535:CDS | 40.0% | |
| AAGGATAGTCGGTATACTAG+AGG | + | chr8.3:31302068-31302087 | MS.gene056535:intron | 40.0% | |
| AATCCTTATCCCACTAAGTG+GGG | - | chr8.3:31302214-31302233 | None:intergenic | 40.0% | |
| AGAAACCCTGTAGATGGTTT+GGG | + | chr8.3:31301994-31302013 | MS.gene056535:intron | 40.0% | |
| GCTGCAAAGATAACATTAGC+AGG | - | chr8.3:31301761-31301780 | None:intergenic | 40.0% | |
| TCTGACTTTCAAAGAGAAGC+TGG | + | chr8.3:31302724-31302743 | MS.gene056535:CDS | 40.0% | |
| TTGAAGTTAGCACGTTCTTG+TGG | + | chr8.3:31302643-31302662 | MS.gene056535:CDS | 40.0% | |
| ! | CTCCATAAGTTGGATGAACA+GGG | - | chr8.3:31301595-31301614 | None:intergenic | 40.0% |
| ! | CTTTTTGTTGCCAAAAGAGC+TGG | - | chr8.3:31302616-31302635 | None:intergenic | 40.0% |
| !!! | GGTATCACTCTTGTTGGTTT+TGG | + | chr8.3:31301725-31301744 | MS.gene056535:CDS | 40.0% |
| AAGATGCCCAAACCATCTAC+AGG | - | chr8.3:31302003-31302022 | None:intergenic | 45.0% | |
| ACAACTTCAGGACCAAATCC+AGG | - | chr8.3:31301665-31301684 | None:intergenic | 45.0% | |
| AGATGCCCAAACCATCTACA+GGG | - | chr8.3:31302002-31302021 | None:intergenic | 45.0% | |
| CAGGCAGAAGTAGTAACCAA+AGG | - | chr8.3:31302826-31302845 | None:intergenic | 45.0% | |
| CAGTTATTCAGCCCTCGTAA+AGG | + | chr8.3:31302871-31302890 | MS.gene056535:CDS | 45.0% | |
| CATGAAGAACAGCTTGCCTT+TGG | + | chr8.3:31302807-31302826 | MS.gene056535:CDS | 45.0% | |
| CCAACATAGCCAACAACTTC+AGG | - | chr8.3:31301677-31301696 | None:intergenic | 45.0% | |
| CGTTCTTGTGGTCATCTTTC+TGG | + | chr8.3:31302655-31302674 | MS.gene056535:CDS | 45.0% | |
| CTTTACGAGGGCTGAATAAC+TGG | - | chr8.3:31302873-31302892 | None:intergenic | 45.0% | |
| GAGAAACCCTGTAGATGGTT+TGG | + | chr8.3:31301993-31302012 | MS.gene056535:intron | 45.0% | |
| TCTAATCCAAACATCCCCTG+AGG | - | chr8.3:31302689-31302708 | None:intergenic | 45.0% | |
| ! | CCTGAAGTTGTTGGCTATGT+TGG | + | chr8.3:31301674-31301693 | MS.gene056535:CDS | 45.0% |
| ! | CTTTGAAAGTCAGACACGGA+AGG | - | chr8.3:31302718-31302737 | None:intergenic | 45.0% |
| ! | GCTCCATAAGTTGGATGAAC+AGG | - | chr8.3:31301596-31301615 | None:intergenic | 45.0% |
| ! | GGATTTGGTCCTGAAGTTGT+TGG | + | chr8.3:31301665-31301684 | MS.gene056535:CDS | 45.0% |
| ACTAAGTGGGGTCGACAACA+TGG | - | chr8.3:31302202-31302221 | None:intergenic | 50.0% | |
| AGTCGGTATACTAGAGGCAG+AGG | + | chr8.3:31302074-31302093 | MS.gene056535:intron | 50.0% | |
| ATGTTGTCGACCCCACTTAG+TGG | + | chr8.3:31302201-31302220 | MS.gene056535:intron | 50.0% | |
| TGTTGTCGACCCCACTTAGT+GGG | + | chr8.3:31302202-31302221 | MS.gene056535:intron | 50.0% | |
| ! | CGTTGGTTGTGGTGTTGGAT+GGG | + | chr8.3:31301637-31301656 | MS.gene056535:CDS | 50.0% |
| ! | GTTGGTTGTGGTGTTGGATG+GGG | + | chr8.3:31301638-31301657 | MS.gene056535:CDS | 50.0% |
| ! | GTTGTTGGCTATGTTGGAGC+TGG | + | chr8.3:31301680-31301699 | MS.gene056535:CDS | 50.0% |
| ! | TATGGAATGGGTTTCGGCGT+TGG | + | chr8.3:31301620-31301639 | MS.gene056535:CDS | 50.0% |
| ! | TGTGGCGTTGGGTTCAATGT+TGG | + | chr8.3:31301704-31301723 | MS.gene056535:CDS | 50.0% |
| ! | TTTCTGGTCCACAAGCCTCA+GGG | + | chr8.3:31302671-31302690 | MS.gene056535:CDS | 50.0% |
| CAAACATCCCCTGAGGCTTG+TGG | - | chr8.3:31302682-31302701 | None:intergenic | 55.0% | |
| CACAAGCCTCAGGGGATGTT+TGG | + | chr8.3:31302680-31302699 | MS.gene056535:CDS | 55.0% | |
| CGACCCCACTTAGTGGGATA+AGG | + | chr8.3:31302208-31302227 | MS.gene056535:intron | 55.0% | |
| GGCTATGTTGGAGCTGGTTG+TGG | + | chr8.3:31301686-31301705 | MS.gene056535:CDS | 55.0% | |
| GTTGGATGGGGTCCTGGATT+TGG | + | chr8.3:31301650-31301669 | MS.gene056535:CDS | 55.0% | |
| TTCTGGTCCACAAGCCTCAG+GGG | + | chr8.3:31302672-31302691 | MS.gene056535:CDS | 55.0% | |
| TTGGAGCTGGTTGTGGCGTT+GGG | + | chr8.3:31301693-31301712 | MS.gene056535:CDS | 55.0% | |
| ! | CTTTCTGGTCCACAAGCCTC+AGG | + | chr8.3:31302670-31302689 | MS.gene056535:CDS | 55.0% |
| !! | ATGGGTTTCGGCGTTGGTTG+TGG | + | chr8.3:31301626-31301645 | MS.gene056535:CDS | 55.0% |
| !! | GCGTTGGTTGTGGTGTTGGA+TGG | + | chr8.3:31301636-31301655 | MS.gene056535:CDS | 55.0% |
| !! | TTCGGCGTTGGTTGTGGTGT+TGG | + | chr8.3:31301632-31301651 | MS.gene056535:CDS | 55.0% |
| GTTGGAGCTGGTTGTGGCGT+TGG | + | chr8.3:31301692-31301711 | MS.gene056535:CDS | 60.0% | |
| ! | TGTGGTGTTGGATGGGGTCC+TGG | + | chr8.3:31301644-31301663 | MS.gene056535:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 31301551 | 31302909 | 31301551 | ID=MS.gene056535 |
| chr8.3 | mRNA | 31301551 | 31302909 | 31301551 | ID=MS.gene056535.t1;Parent=MS.gene056535 |
| chr8.3 | exon | 31301551 | 31301791 | 31301551 | ID=MS.gene056535.t1.exon1;Parent=MS.gene056535.t1 |
| chr8.3 | CDS | 31301551 | 31301791 | 31301551 | ID=cds.MS.gene056535.t1;Parent=MS.gene056535.t1 |
| chr8.3 | exon | 31302617 | 31302909 | 31302617 | ID=MS.gene056535.t1.exon2;Parent=MS.gene056535.t1 |
| chr8.3 | CDS | 31302617 | 31302909 | 31302617 | ID=cds.MS.gene056535.t1;Parent=MS.gene056535.t1 |
| Gene Sequence |
| Protein sequence |
