AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83424


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83424.t1 MTR_8g071180 96.532 173 6 0 1 173 1 173 1.26e-122 343
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83424.t1 AT4G19070 53.179 173 67 3 1 173 4 162 1.04e-55 174
MS.gene83424.t1 AT4G19070 53.179 173 67 3 1 173 1 159 1.35e-55 173

Find 46 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTCTTGTGGTCATCTTTC+TGG 0.048652 8.4:+32427785 MS.gene83424:CDS
GTTCTAATAATGATTATAAA+AGG 0.238226 8.4:+32426674 None:intergenic
CCCTCGTAAAGGTATTATTT+TGG 0.247253 8.4:+32428012 MS.gene83424:CDS
GGTATCACTCTTGTTGGTTT+TGG 0.272786 8.4:+32426857 MS.gene83424:CDS
ATCTTTGCAGCTCCTTATAA+TGG 0.287147 8.4:+32426902 MS.gene83424:CDS
TGTGGTGTTGGATGGGGTCC+TGG 0.292274 8.4:+32426776 MS.gene83424:CDS
GTTGGATGGGGTCCTGGATT+TGG 0.298350 8.4:+32426782 MS.gene83424:CDS
CACAAGCCTCAGGGGATGTT+TGG 0.315024 8.4:+32427810 MS.gene83424:CDS
AACCCTGTTCATCCAACTTA+TGG 0.315421 8.4:+32426722 MS.gene83424:CDS
CTTTACGAGGGCTGAATAAC+TGG 0.320207 8.4:-32428000 None:intergenic
GCTCCATAAGTTGGATGAAC+AGG 0.342945 8.4:-32426725 None:intergenic
CGTTGGTTGTGGTGTTGGAT+GGG 0.354052 8.4:+32426769 MS.gene83424:CDS
AAAAGAGGTATACCATTATA+AGG 0.354158 8.4:-32426914 None:intergenic
TTCGGCGTTGGTTGTGGTGT+TGG 0.368268 8.4:+32426764 MS.gene83424:CDS
AATGTTGGTATCACTCTTGT+TGG 0.381476 8.4:+32426851 MS.gene83424:CDS
GCGTTGGTTGTGGTGTTGGA+TGG 0.382518 8.4:+32426768 MS.gene83424:CDS
CCTGAAGTTGTTGGCTATGT+TGG 0.398231 8.4:+32426806 MS.gene83424:CDS
CCAACATAGCCAACAACTTC+AGG 0.400009 8.4:-32426806 None:intergenic
CTTTCTGGTCCACAAGCCTC+AGG 0.405935 8.4:+32427800 MS.gene83424:CDS
ATGGGTTTCGGCGTTGGTTG+TGG 0.411499 8.4:+32426758 MS.gene83424:CDS
CATGAAGAACAGCTTGCCTT+TGG 0.419512 8.4:+32427937 MS.gene83424:CDS
GTTGGAGCTGGTTGTGGCGT+TGG 0.434987 8.4:+32426824 MS.gene83424:CDS
GGATTTGGTCCTGAAGTTGT+TGG 0.437390 8.4:+32426797 MS.gene83424:CDS
TATGGAATGGGTTTCGGCGT+TGG 0.445305 8.4:+32426752 MS.gene83424:CDS
CCATAAAATGCTCCATAAGT+TGG 0.451487 8.4:-32426734 None:intergenic
GTTGTTGGCTATGTTGGAGC+TGG 0.461109 8.4:+32426812 MS.gene83424:CDS
GTTGGTTGTGGTGTTGGATG+GGG 0.464756 8.4:+32426770 MS.gene83424:CDS
GGCTATGTTGGAGCTGGTTG+TGG 0.466785 8.4:+32426818 MS.gene83424:CDS
TGTTTAGAAGATATGAAAGA+TGG 0.483303 8.4:+32426699 MS.gene83424:CDS
TCTGACTTTCAAAGAGAAGC+TGG 0.521844 8.4:+32427854 MS.gene83424:CDS
CAGTTATTCAGCCCTCGTAA+AGG 0.540196 8.4:+32428001 MS.gene83424:CDS
GCTGCAAAGATAACATTAGC+AGG 0.540585 8.4:-32426890 None:intergenic
TGTGGCGTTGGATTCAATGT+TGG 0.556593 8.4:+32426836 MS.gene83424:CDS
ACAACTTCAGGACCAAATCC+AGG 0.557849 8.4:-32426794 None:intergenic
CAAACATCCCCTGAGGCTTG+TGG 0.576117 8.4:-32427809 None:intergenic
CAAAAGTATTTATCAATCAA+CGG 0.579321 8.4:+32427899 MS.gene83424:CDS
TTTCTGGTCCACAAGCCTCA+GGG 0.581201 8.4:+32427801 MS.gene83424:CDS
TTGAAGTTAGCACGTTCTTG+TGG 0.587715 8.4:+32427773 MS.gene83424:CDS
CTCCATAAGTTGGATGAACA+GGG 0.608622 8.4:-32426724 None:intergenic
CCAAAATAATACCTTTACGA+GGG 0.610802 8.4:-32428012 None:intergenic
TTCTGGTCCACAAGCCTCAG+GGG 0.613524 8.4:+32427802 MS.gene83424:CDS
CTTTGAAAGTCAGACACGGA+AGG 0.618621 8.4:-32427845 None:intergenic
TTCTCTTTGAAAGTCAGACA+CGG 0.624465 8.4:-32427849 None:intergenic
ACCAAAATAATACCTTTACG+AGG 0.635161 8.4:-32428013 None:intergenic
CAGGCAGAAGTAGTAACCAA+AGG 0.639806 8.4:-32427953 None:intergenic
TCTAATCCAAACATCCCCTG+AGG 0.750357 8.4:-32427816 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATGATATTTTTAAGTAA+AGG + chr8.4:32427024-32427043 MS.gene83424:intron 10.0%
!!! TACATTCTTAAATTATTTTC+AGG - chr8.4:32427093-32427112 None:intergenic 15.0%
!! AGTTCAATTTAGTATGAAAT+TGG + chr8.4:32427562-32427581 MS.gene83424:intron 20.0%
!! GTTCAATTTAGTATGAAATT+GGG + chr8.4:32427563-32427582 MS.gene83424:intron 20.0%
!! TGGTTTATGATAGAATATTA+TGG + chr8.4:32427297-32427316 MS.gene83424:intron 20.0%
!!! CAAAAGTATTTATCAATCAA+CGG + chr8.4:32427899-32427918 MS.gene83424:CDS 20.0%
! AGATATAGAGATTAATGAGT+TGG + chr8.4:32427265-32427284 MS.gene83424:intron 25.0%
! ATACACAGTTGACAAAAAAA+TGG + chr8.4:32427399-32427418 MS.gene83424:intron 25.0%
! ATTGATGCAAATATTATGTG+TGG + chr8.4:32427627-32427646 MS.gene83424:intron 25.0%
! TGTTTAGAAGATATGAAAGA+TGG + chr8.4:32426699-32426718 MS.gene83424:CDS 25.0%
! TTGGAATTATCATTGTGAAA+CGG + chr8.4:32427462-32427481 MS.gene83424:intron 25.0%
!! AAAAGAGGTATACCATTATA+AGG - chr8.4:32426917-32426936 None:intergenic 25.0%
!! AAATGCTTGTATACTTTTAC+AGG - chr8.4:32427975-32427994 None:intergenic 25.0%
!! TCTCTAATGTGTATCTATTT+TGG - chr8.4:32427529-32427548 None:intergenic 25.0%
!! TTTTGTCAACTGTGTATATT+CGG - chr8.4:32427396-32427415 None:intergenic 25.0%
!!! AATGATTCTTTGGAATGAAT+TGG + chr8.4:32427443-32427462 MS.gene83424:intron 25.0%
!!! GTGAAAAATTCTCTTGTTTT+CGG + chr8.4:32427876-32427895 MS.gene83424:CDS 25.0%
AAAGAATCATTACGTAAACG+TGG - chr8.4:32427434-32427453 None:intergenic 30.0%
ACCAAAATAATACCTTTACG+AGG - chr8.4:32428016-32428035 None:intergenic 30.0%
ACGTTTACGTAATGATTCTT+TGG + chr8.4:32427433-32427452 MS.gene83424:intron 30.0%
CAAAAGAGCTGGATTAAAAT+AGG - chr8.4:32427735-32427754 None:intergenic 30.0%
CCAAAATAATACCTTTACGA+GGG - chr8.4:32428015-32428034 None:intergenic 30.0%
TAGATAAGATGAAGGATAGT+CGG + chr8.4:32427189-32427208 MS.gene83424:intron 30.0%
TGGAATTATCATTGTGAAAC+GGG + chr8.4:32427463-32427482 MS.gene83424:intron 30.0%
! CTATTTTCTGAATCGTGAAT+CGG + chr8.4:32426981-32427000 MS.gene83424:intron 30.0%
! TAGAGATTAATGAGTTGGAT+CGG + chr8.4:32427270-32427289 MS.gene83424:intron 30.0%
!! TATGGAGCATTTTATGGAAT+GGG + chr8.4:32426740-32426759 MS.gene83424:CDS 30.0%
!! TTATGGAGCATTTTATGGAA+TGG + chr8.4:32426739-32426758 MS.gene83424:CDS 30.0%
!!! CTATTTTAATCCAGCTCTTT+TGG + chr8.4:32427733-32427752 MS.gene83424:intron 30.0%
AAAATCCTTATCCCACTAAG+TGG - chr8.4:32427348-32427367 None:intergenic 35.0%
AAATCCTTATCCCACTAAGT+GGG - chr8.4:32427347-32427366 None:intergenic 35.0%
AACGAGAGTAGATAAGATGA+AGG + chr8.4:32427181-32427200 MS.gene83424:intron 35.0%
AATGTTGGTATCACTCTTGT+TGG + chr8.4:32426851-32426870 MS.gene83424:CDS 35.0%
ATCTTTGCAGCTCCTTATAA+TGG + chr8.4:32426902-32426921 MS.gene83424:CDS 35.0%
CCATAAAATGCTCCATAAGT+TGG - chr8.4:32426737-32426756 None:intergenic 35.0%
CTCGTTACTACAAAATCAAC+AGG - chr8.4:32427167-32427186 None:intergenic 35.0%
GCGTAACAATCAACAAAAAG+AGG - chr8.4:32426932-32426951 None:intergenic 35.0%
TATAAGAGAAACCCTGTAGA+TGG + chr8.4:32427120-32427139 MS.gene83424:intron 35.0%
! ACTTAGTGGGATAAGGATTT+TGG + chr8.4:32427347-32427366 MS.gene83424:intron 35.0%
! TAATGAGTTGGATCGGAATA+TGG + chr8.4:32427277-32427296 MS.gene83424:intron 35.0%
! TTCTCTTTGAAAGTCAGACA+CGG - chr8.4:32427852-32427871 None:intergenic 35.0%
!! CCAACTTATGGAGCATTTTA+TGG + chr8.4:32426734-32426753 MS.gene83424:CDS 35.0%
!! GCATTTTATGGAATGGGTTT+CGG + chr8.4:32426746-32426765 MS.gene83424:CDS 35.0%
!!! ACTCTTGTTGGTTTTGGAAT+TGG + chr8.4:32426863-32426882 MS.gene83424:CDS 35.0%
!!! CCCTCGTAAAGGTATTATTT+TGG + chr8.4:32428012-32428031 MS.gene83424:CDS 35.0%
AACCCTGTTCATCCAACTTA+TGG + chr8.4:32426722-32426741 MS.gene83424:CDS 40.0%
AAGGATAGTCGGTATACTAG+AGG + chr8.4:32427200-32427219 MS.gene83424:intron 40.0%
AATCCTTATCCCACTAAGTG+GGG - chr8.4:32427346-32427365 None:intergenic 40.0%
AGAAACCCTGTAGATGGTTT+GGG + chr8.4:32427126-32427145 MS.gene83424:intron 40.0%
GCTGCAAAGATAACATTAGC+AGG - chr8.4:32426893-32426912 None:intergenic 40.0%
TCTGACTTTCAAAGAGAAGC+TGG + chr8.4:32427854-32427873 MS.gene83424:CDS 40.0%
TTGAAGTTAGCACGTTCTTG+TGG + chr8.4:32427773-32427792 MS.gene83424:CDS 40.0%
! CTCCATAAGTTGGATGAACA+GGG - chr8.4:32426727-32426746 None:intergenic 40.0%
! CTTTTTGTTGCCAAAAGAGC+TGG - chr8.4:32427746-32427765 None:intergenic 40.0%
!!! GGTATCACTCTTGTTGGTTT+TGG + chr8.4:32426857-32426876 MS.gene83424:CDS 40.0%
AAGATGCCCAAACCATCTAC+AGG - chr8.4:32427135-32427154 None:intergenic 45.0%
ACAACTTCAGGACCAAATCC+AGG - chr8.4:32426797-32426816 None:intergenic 45.0%
AGATGCCCAAACCATCTACA+GGG - chr8.4:32427134-32427153 None:intergenic 45.0%
CAGGCAGAAGTAGTAACCAA+AGG - chr8.4:32427956-32427975 None:intergenic 45.0%
CAGTTATTCAGCCCTCGTAA+AGG + chr8.4:32428001-32428020 MS.gene83424:CDS 45.0%
CATGAAGAACAGCTTGCCTT+TGG + chr8.4:32427937-32427956 MS.gene83424:CDS 45.0%
CCAACATAGCCAACAACTTC+AGG - chr8.4:32426809-32426828 None:intergenic 45.0%
CGTTCTTGTGGTCATCTTTC+TGG + chr8.4:32427785-32427804 MS.gene83424:CDS 45.0%
CTTTACGAGGGCTGAATAAC+TGG - chr8.4:32428003-32428022 None:intergenic 45.0%
GAGAAACCCTGTAGATGGTT+TGG + chr8.4:32427125-32427144 MS.gene83424:intron 45.0%
TCTAATCCAAACATCCCCTG+AGG - chr8.4:32427819-32427838 None:intergenic 45.0%
! CCTGAAGTTGTTGGCTATGT+TGG + chr8.4:32426806-32426825 MS.gene83424:CDS 45.0%
! CTTTGAAAGTCAGACACGGA+AGG - chr8.4:32427848-32427867 None:intergenic 45.0%
! GCTCCATAAGTTGGATGAAC+AGG - chr8.4:32426728-32426747 None:intergenic 45.0%
! GGATTTGGTCCTGAAGTTGT+TGG + chr8.4:32426797-32426816 MS.gene83424:CDS 45.0%
! TGTGGCGTTGGATTCAATGT+TGG + chr8.4:32426836-32426855 MS.gene83424:CDS 45.0%
ACTAAGTGGGGTCGACAACA+TGG - chr8.4:32427334-32427353 None:intergenic 50.0%
AGTCGGTATACTAGAGGCAG+AGG + chr8.4:32427206-32427225 MS.gene83424:intron 50.0%
ATGTTGTCGACCCCACTTAG+TGG + chr8.4:32427333-32427352 MS.gene83424:intron 50.0%
TGTTGTCGACCCCACTTAGT+GGG + chr8.4:32427334-32427353 MS.gene83424:intron 50.0%
! CGTTGGTTGTGGTGTTGGAT+GGG + chr8.4:32426769-32426788 MS.gene83424:CDS 50.0%
! GTTGGTTGTGGTGTTGGATG+GGG + chr8.4:32426770-32426789 MS.gene83424:CDS 50.0%
! GTTGTTGGCTATGTTGGAGC+TGG + chr8.4:32426812-32426831 MS.gene83424:CDS 50.0%
! TATGGAATGGGTTTCGGCGT+TGG + chr8.4:32426752-32426771 MS.gene83424:CDS 50.0%
! TTTCTGGTCCACAAGCCTCA+GGG + chr8.4:32427801-32427820 MS.gene83424:CDS 50.0%
CAAACATCCCCTGAGGCTTG+TGG - chr8.4:32427812-32427831 None:intergenic 55.0%
CACAAGCCTCAGGGGATGTT+TGG + chr8.4:32427810-32427829 MS.gene83424:CDS 55.0%
CGACCCCACTTAGTGGGATA+AGG + chr8.4:32427340-32427359 MS.gene83424:intron 55.0%
GGCTATGTTGGAGCTGGTTG+TGG + chr8.4:32426818-32426837 MS.gene83424:CDS 55.0%
GTTGGATGGGGTCCTGGATT+TGG + chr8.4:32426782-32426801 MS.gene83424:CDS 55.0%
TTCTGGTCCACAAGCCTCAG+GGG + chr8.4:32427802-32427821 MS.gene83424:CDS 55.0%
! CTTTCTGGTCCACAAGCCTC+AGG + chr8.4:32427800-32427819 MS.gene83424:CDS 55.0%
!! ATGGGTTTCGGCGTTGGTTG+TGG + chr8.4:32426758-32426777 MS.gene83424:CDS 55.0%
!! GCGTTGGTTGTGGTGTTGGA+TGG + chr8.4:32426768-32426787 MS.gene83424:CDS 55.0%
!! TTCGGCGTTGGTTGTGGTGT+TGG + chr8.4:32426764-32426783 MS.gene83424:CDS 55.0%
GTTGGAGCTGGTTGTGGCGT+TGG + chr8.4:32426824-32426843 MS.gene83424:CDS 60.0%
! TGTGGTGTTGGATGGGGTCC+TGG + chr8.4:32426776-32426795 MS.gene83424:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 32426683 32428039 32426683 ID=MS.gene83424
chr8.4 mRNA 32426683 32428039 32426683 ID=MS.gene83424.t1;Parent=MS.gene83424
chr8.4 exon 32426683 32426923 32426683 ID=MS.gene83424.t1.exon1;Parent=MS.gene83424.t1
chr8.4 CDS 32426683 32426923 32426683 ID=cds.MS.gene83424.t1;Parent=MS.gene83424.t1
chr8.4 exon 32427747 32428039 32427747 ID=MS.gene83424.t1.exon2;Parent=MS.gene83424.t1
chr8.4 CDS 32427747 32428039 32427747 ID=cds.MS.gene83424.t1;Parent=MS.gene83424.t1
Gene Sequence

>MS.gene83424

ATGATTATAAAAGGATTGTTTAGAAGATATGAAAGATGGAACCCTGTTCATCCAACTTATGGAGCATTTTATGGAATGGGTTTCGGCGTTGGTTGTGGTGTTGGATGGGGTCCTGGATTTGGTCCTGAAGTTGTTGGCTATGTTGGAGCTGGTTGTGGCGTTGGATTCAATGTTGGTATCACTCTTGTTGGTTTTGGAATTGGTCTTCCTGCTAATGTTATCTTTGCAGCTCCTTATAATGGTATACCTCTTTTTGTTGATTGTTACGCAAAATCATTCATATTGTGAATCAGAAGAACTATTTTCTGAATCGTGAATCGGATTTTTATCGCAGCTATAAAAATATGATATTTTTAAGTAAAGGCAAGTGACATAACTATGTTATAAGTTGATATATCATATATGAACCTGAAAATAATTTAAGAATGTACGAAGACTATAAGAGAAACCCTGTAGATGGTTTGGGCATCTTGAGAGAAAACCTGTTGATTTTGTAGTAACGAGAGTAGATAAGATGAAGGATAGTCGGTATACTAGAGGCAGAGGTAGACTTAGAAAAACTATAAGAGAAATTATTCAGAAAGATATAGAGATTAATGAGTTGGATCGGAATATGGTTTATGATAGAATATTATGGCGTAATTTGATCCATGTTGTCGACCCCACTTAGTGGGATAAGGATTTTGGTTGTTGTTGTCATAAGAAATTGACCGAATATACACAGTTGACAAAAAAATGGCTCACTACACCACGTTTACGTAATGATTCTTTGGAATGAATTGGAATTATCATTGTGAAACGGGATTCAAAATTATGAATAAGAATTCAAAATGAGATTCATGTCCAAAATAGATACACATTAGAGATTCGTTTCGATTGAGTTCAATTTAGTATGAAATTGGGATACAACTCGCATGTAGTAAGATACTGATAACTATGCTCAAATTGATGCAAATATTATGTGTGGTTAGAATCTACGATTCTTAGTCTTTCTTAAAAATAAAAAAAATCTACGATTCTTAGCTGAAACTTATTCACATTATTTGTGACCTATTTTAATCCAGCTCTTTTGGCAACAAAAAGCTCTGCATTGAAGTTAGCACGTTCTTGTGGTCATCTTTCTGGTCCACAAGCCTCAGGGGATGTTTGGATTAGAAGTGTACCTTCCGTGTCTGACTTTCAAAGAGAAGCTGGTGAAAAATTCTCTTGTTTTCGGCAAAAGTATTTATCAATCAACGGAACTGATTTCTTTGACATGAAGAACAGCTTGCCTTTGGTTACTACTTCTGCCTGTAAAAGTATACAAGCATTTCATAGCCAGTTATTCAGCCCTCGTAAAGGTATTATTTTGGTTTAG

Protein sequence

>MS.gene83424.t1

MIIKGLFRRYERWNPVHPTYGAFYGMGFGVGCGVGWGPGFGPEVVGYVGAGCGVGFNVGITLVGFGIGLPANVIFAAPYNALLATKSSALKLARSCGHLSGPQASGDVWIRSVPSVSDFQREAGEKFSCFRQKYLSINGTDFFDMKNSLPLVTTSACKSIQAFHSQLFSPRKGIILV