Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057115.t1 | AES72521.1 | 88 | 242 | 16 | 4 | 1 | 238 | 29 | 261 | 1.40E-110 | 409.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057115.t1 | Q9LVU8 | 40.3 | 201 | 105 | 3 | 43 | 232 | 53 | 249 | 7.6e-28 | 125.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057115.t1 | G7J7G0 | 88.0 | 242 | 16 | 4 | 1 | 238 | 29 | 261 | 1.0e-110 | 409.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057115 | MS.gene057117 | 0.880129 | 7.08E-70 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTTAGCAGCCATTGTTAA+AGG | 0.208533 | 3.1:+73608292 | None:intergenic |
| AATGTTGTCTTGCCTGAGTT+GGG | 0.325768 | 3.1:-73608544 | MS.gene057115:CDS |
| CAATGTTGTCTTGCCTGAGT+TGG | 0.335905 | 3.1:-73608545 | MS.gene057115:CDS |
| ATGATTTCTTTGAGTTTGAT+TGG | 0.342407 | 3.1:-73608696 | MS.gene057115:CDS |
| GAAGATGTTGGAGAAACTTC+CGG | 0.360968 | 3.1:-73607405 | MS.gene057115:CDS |
| CAGCAATAGTCTCACCTTTG+TGG | 0.390633 | 3.1:+73608512 | None:intergenic |
| AAGATGTTGGAGAAACTTCC+GGG | 0.393818 | 3.1:-73607404 | MS.gene057115:CDS |
| TCATCATTCAAATCCATTCC+CGG | 0.427588 | 3.1:+73607386 | None:intergenic |
| TTGTGAAGAAAGAGAAGGAA+TGG | 0.429732 | 3.1:-73608441 | MS.gene057115:CDS |
| AGAATTGTCATTAGTTCCAT+TGG | 0.430301 | 3.1:-73608410 | MS.gene057115:CDS |
| CAGAAAACTGAGGAGCTTGA+TGG | 0.447810 | 3.1:-73608203 | MS.gene057115:CDS |
| GTACGAGTACTAGCATCATT+AGG | 0.450426 | 3.1:-73608366 | MS.gene057115:CDS |
| CTCGTACTGCTACTTGTACT+TGG | 0.454025 | 3.1:+73608382 | None:intergenic |
| GGTACTCATCGACAACCTTA+AGG | 0.457457 | 3.1:-73608182 | MS.gene057115:intron |
| GTTGGAGAAACTTCCGGGAA+TGG | 0.462988 | 3.1:-73607399 | MS.gene057115:CDS |
| CCTCAAAATTATTCTGATCA+AGG | 0.463476 | 3.1:+73608584 | None:intergenic |
| TGATTTCTTTGAGTTTGATT+GGG | 0.465378 | 3.1:-73608695 | MS.gene057115:CDS |
| TGTGAAGAAAGAGAAGGAAT+GGG | 0.479018 | 3.1:-73608440 | MS.gene057115:CDS |
| TGTTGTGTGATGCAGGAAAA+GGG | 0.482015 | 3.1:-73607464 | MS.gene057115:intron |
| ATGATGAAATCAAGAAGCTT+AGG | 0.499327 | 3.1:-73607370 | MS.gene057115:CDS |
| CTTGTACTTGGCAATGCCAA+TGG | 0.515278 | 3.1:+73608394 | None:intergenic |
| GGAGAAGCAAAAGAAGATGT+TGG | 0.518512 | 3.1:-73607417 | MS.gene057115:CDS |
| GCTTCTGTGAAAACTGAAAG+TGG | 0.520217 | 3.1:-73608466 | MS.gene057115:CDS |
| AACTCAAAGAAATCATCAAA+CGG | 0.523633 | 3.1:+73608703 | None:intergenic |
| GACTAAGGAAGTTGCCATGT+TGG | 0.527747 | 3.1:-73607438 | MS.gene057115:CDS |
| ATGTTGTCTTGCCTGAGTTG+GGG | 0.533975 | 3.1:-73608543 | MS.gene057115:CDS |
| GCAGGAAAAGGGTTTGACTA+AGG | 0.541768 | 3.1:-73607453 | MS.gene057115:intron |
| TGGTTGTAATGCCCCAACTC+AGG | 0.557980 | 3.1:+73608532 | None:intergenic |
| AATTATTCTGATCAAGGTGT+GGG | 0.571228 | 3.1:+73608590 | None:intergenic |
| CTCAGGCAAGACAACATTGA+GGG | 0.577274 | 3.1:+73608549 | None:intergenic |
| GTACTAGCATCATTAGGAAG+AGG | 0.587268 | 3.1:-73608360 | MS.gene057115:CDS |
| TATTCTGATCAAGGTGTGGG+AGG | 0.607927 | 3.1:+73608593 | None:intergenic |
| AAATTATTCTGATCAAGGTG+TGG | 0.609905 | 3.1:+73608589 | None:intergenic |
| GCAGGGAGCTCAATATCACT+AGG | 0.629741 | 3.1:-73608234 | MS.gene057115:CDS |
| GCTGCTAAGAAAATGAATGT+TGG | 0.631864 | 3.1:-73608280 | MS.gene057115:CDS |
| TCAGGCAAGACAACATTGAG+GGG | 0.654617 | 3.1:+73608550 | None:intergenic |
| ACTATTGCTGATGCTGAGAG+TGG | 0.657284 | 3.1:-73608499 | MS.gene057115:CDS |
| ACTCAGGCAAGACAACATTG+AGG | 0.692460 | 3.1:+73608548 | None:intergenic |
| GGGAGCTCAATATCACTAGG+TGG | 0.701034 | 3.1:-73608231 | MS.gene057115:CDS |
| TTGGGGCATTACAACCACAA+AGG | 0.718163 | 3.1:-73608526 | MS.gene057115:CDS |
| GGTGGCCTCACAGAAAACTG+AGG | 0.738213 | 3.1:-73608213 | MS.gene057115:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTAAAAAACATAAAAAAAT+TGG | + | chr3.1:73608183-73608202 | None:intergenic | 10.0% |
| !! | AAAGAAAAAGAATATTCTTA+AGG | + | chr3.1:73608107-73608126 | None:intergenic | 15.0% |
| !! | ATTAGTTATTTATTGTCAAA+TGG | - | chr3.1:73608525-73608544 | MS.gene057115:CDS | 15.0% |
| !! | TCATGTAAAAATGATTACTT+TGG | + | chr3.1:73607379-73607398 | None:intergenic | 20.0% |
| ! | AAACCATTATTTACTCTGTT+TGG | - | chr3.1:73607927-73607946 | MS.gene057115:intron | 25.0% |
| ! | AACTCAAAGAAATCATCAAA+CGG | + | chr3.1:73607332-73607351 | None:intergenic | 25.0% |
| ! | AATCCAAACAGAGTAAATAA+TGG | + | chr3.1:73607933-73607952 | None:intergenic | 25.0% |
| ! | ACAACATAGAATATACCTTA+AGG | + | chr3.1:73607868-73607887 | None:intergenic | 25.0% |
| ! | ACACATCTTATATTCTCTTT+AGG | - | chr3.1:73608206-73608225 | MS.gene057115:CDS | 25.0% |
| ! | CATTTCAAATACATCTACAA+TGG | - | chr3.1:73608231-73608250 | MS.gene057115:CDS | 25.0% |
| ! | TAAAGAGAATATAAGATGTG+TGG | + | chr3.1:73608207-73608226 | None:intergenic | 25.0% |
| ! | TTTCATGTACTTGAAAATGT+GGG | + | chr3.1:73607910-73607929 | None:intergenic | 25.0% |
| !! | ATGATTTCTTTGAGTTTGAT+TGG | - | chr3.1:73607336-73607355 | MS.gene057115:CDS | 25.0% |
| !! | TATTCTATGTTGTTTGAGTT+TGG | - | chr3.1:73607875-73607894 | MS.gene057115:intron | 25.0% |
| !! | TGATTTCTTTGAGTTTGATT+GGG | - | chr3.1:73607337-73607356 | MS.gene057115:CDS | 25.0% |
| !! | TTTTGATGTTCCTTTAACAA+TGG | - | chr3.1:73607730-73607749 | MS.gene057115:intron | 25.0% |
| AAATTATTCTGATCAAGGTG+TGG | + | chr3.1:73607446-73607465 | None:intergenic | 30.0% | |
| AATTATTCTGATCAAGGTGT+GGG | + | chr3.1:73607445-73607464 | None:intergenic | 30.0% | |
| AGAATTGTCATTAGTTCCAT+TGG | - | chr3.1:73607622-73607641 | MS.gene057115:intron | 30.0% | |
| ATGATGAAATCAAGAAGCTT+AGG | - | chr3.1:73608662-73608681 | MS.gene057115:CDS | 30.0% | |
| CCTCAAAATTATTCTGATCA+AGG | + | chr3.1:73607451-73607470 | None:intergenic | 30.0% | |
| GTTTCATGTACTTGAAAATG+TGG | + | chr3.1:73607911-73607930 | None:intergenic | 30.0% | |
| TGGGAATGATAATTGAAACA+TGG | - | chr3.1:73608001-73608020 | MS.gene057115:intron | 30.0% | |
| TTAGTTTCTTCGATAGTAGA+TGG | - | chr3.1:73608325-73608344 | MS.gene057115:CDS | 30.0% | |
| TTCATGTACTTGAAAATGTG+GGG | + | chr3.1:73607909-73607928 | None:intergenic | 30.0% | |
| ! | CCTTGATCAGAATAATTTTG+AGG | - | chr3.1:73607448-73607467 | MS.gene057115:CDS | 30.0% |
| ! | TGTACCTTAGACTAAGTTTT+TGG | - | chr3.1:73607966-73607985 | MS.gene057115:intron | 30.0% |
| !! | CCTTTTGAAAAAAAGATGCA+GGG | - | chr3.1:73607781-73607800 | MS.gene057115:intron | 30.0% |
| !!! | CCTGCATCTTTTTTTCAAAA+GGG | + | chr3.1:73607783-73607802 | None:intergenic | 30.0% |
| CTATGAAACATGACAACTCT+AGG | - | chr3.1:73608052-73608071 | MS.gene057115:intron | 35.0% | |
| GCTGCTAAGAAAATGAATGT+TGG | - | chr3.1:73607752-73607771 | MS.gene057115:intron | 35.0% | |
| GTTTCAATTATCATTCCCAG+CGG | + | chr3.1:73608000-73608019 | None:intergenic | 35.0% | |
| TCATCATTCAAATCCATTCC+CGG | + | chr3.1:73608649-73608668 | None:intergenic | 35.0% | |
| TGGACCAAAAACTTAGTCTA+AGG | + | chr3.1:73607973-73607992 | None:intergenic | 35.0% | |
| TGTGAAGAAAGAGAAGGAAT+GGG | - | chr3.1:73607592-73607611 | MS.gene057115:intron | 35.0% | |
| TTCTTAGCAGCCATTGTTAA+AGG | + | chr3.1:73607743-73607762 | None:intergenic | 35.0% | |
| TTGTGAAGAAAGAGAAGGAA+TGG | - | chr3.1:73607591-73607610 | MS.gene057115:intron | 35.0% | |
| TTTCAATTATCATTCCCAGC+GGG | + | chr3.1:73607999-73608018 | None:intergenic | 35.0% | |
| ! | AAAATGTATCGACGCAACAA+TGG | - | chr3.1:73608266-73608285 | MS.gene057115:CDS | 35.0% |
| ! | CTTTGTTTCGACACTTAGAT+CGG | - | chr3.1:73608123-73608142 | MS.gene057115:intron | 35.0% |
| !! | ATTGTTTTGTTGTGTGATGC+AGG | - | chr3.1:73608561-73608580 | MS.gene057115:CDS | 35.0% |
| !! | CCCTTTTGAAAAAAAGATGC+AGG | - | chr3.1:73607780-73607799 | MS.gene057115:intron | 35.0% |
| !!! | CCCTGCATCTTTTTTTCAAA+AGG | + | chr3.1:73607784-73607803 | None:intergenic | 35.0% |
| !!! | TGGTTTTGTGAAGAAAGAGA+AGG | - | chr3.1:73607586-73607605 | MS.gene057115:intron | 35.0% |
| AAGATGTTGGAGAAACTTCC+GGG | - | chr3.1:73608628-73608647 | MS.gene057115:CDS | 40.0% | |
| AATGTTGTCTTGCCTGAGTT+GGG | - | chr3.1:73607488-73607507 | MS.gene057115:intron | 40.0% | |
| GAAGATGTTGGAGAAACTTC+CGG | - | chr3.1:73608627-73608646 | MS.gene057115:CDS | 40.0% | |
| GCTTCTGTGAAAACTGAAAG+TGG | - | chr3.1:73607566-73607585 | MS.gene057115:intron | 40.0% | |
| GGAGAAGCAAAAGAAGATGT+TGG | - | chr3.1:73608615-73608634 | MS.gene057115:CDS | 40.0% | |
| GTACGAGTACTAGCATCATT+AGG | - | chr3.1:73607666-73607685 | MS.gene057115:intron | 40.0% | |
| GTACTAGCATCATTAGGAAG+AGG | - | chr3.1:73607672-73607691 | MS.gene057115:intron | 40.0% | |
| TGTTGTGTGATGCAGGAAAA+GGG | - | chr3.1:73608568-73608587 | MS.gene057115:CDS | 40.0% | |
| TTGTTGTGTGATGCAGGAAA+AGG | - | chr3.1:73608567-73608586 | MS.gene057115:CDS | 40.0% | |
| ! | CTTCTTTTGCTTCTCCAACA+TGG | + | chr3.1:73608611-73608630 | None:intergenic | 40.0% |
| ACTATTGCTGATGCTGAGAG+TGG | - | chr3.1:73607533-73607552 | MS.gene057115:intron | 45.0% | |
| ACTCAGGCAAGACAACATTG+AGG | + | chr3.1:73607487-73607506 | None:intergenic | 45.0% | |
| ATGTTGTCTTGCCTGAGTTG+GGG | - | chr3.1:73607489-73607508 | MS.gene057115:intron | 45.0% | |
| CAATGTTGTCTTGCCTGAGT+TGG | - | chr3.1:73607487-73607506 | MS.gene057115:intron | 45.0% | |
| CAGAAAACTGAGGAGCTTGA+TGG | - | chr3.1:73607829-73607848 | MS.gene057115:intron | 45.0% | |
| CAGCAATAGTCTCACCTTTG+TGG | + | chr3.1:73607523-73607542 | None:intergenic | 45.0% | |
| CTCAGGCAAGACAACATTGA+GGG | + | chr3.1:73607486-73607505 | None:intergenic | 45.0% | |
| CTTGTACTTGGCAATGCCAA+TGG | + | chr3.1:73607641-73607660 | None:intergenic | 45.0% | |
| GACTAAGGAAGTTGCCATGT+TGG | - | chr3.1:73608594-73608613 | MS.gene057115:CDS | 45.0% | |
| GCAGGAAAAGGGTTTGACTA+AGG | - | chr3.1:73608579-73608598 | MS.gene057115:CDS | 45.0% | |
| GGTACTCATCGACAACCTTA+AGG | - | chr3.1:73607850-73607869 | MS.gene057115:intron | 45.0% | |
| TATTCTGATCAAGGTGTGGG+AGG | + | chr3.1:73607442-73607461 | None:intergenic | 45.0% | |
| TCAGGCAAGACAACATTGAG+GGG | + | chr3.1:73607485-73607504 | None:intergenic | 45.0% | |
| ! | AAGCTCCTCAGTTTTCTGTG+AGG | + | chr3.1:73607827-73607846 | None:intergenic | 45.0% |
| ! | CTCGTACTGCTACTTGTACT+TGG | + | chr3.1:73607653-73607672 | None:intergenic | 45.0% |
| ! | TTGGGGCATTACAACCACAA+AGG | - | chr3.1:73607506-73607525 | MS.gene057115:intron | 45.0% |
| GCAGGGAGCTCAATATCACT+AGG | - | chr3.1:73607798-73607817 | MS.gene057115:intron | 50.0% | |
| GGGAGCTCAATATCACTAGG+TGG | - | chr3.1:73607801-73607820 | MS.gene057115:intron | 50.0% | |
| GTTGGAGAAACTTCCGGGAA+TGG | - | chr3.1:73608633-73608652 | MS.gene057115:CDS | 50.0% | |
| TGGTTGTAATGCCCCAACTC+AGG | + | chr3.1:73607503-73607522 | None:intergenic | 50.0% | |
| TTATCATTCCCAGCGGGTCA+TGG | + | chr3.1:73607993-73608012 | None:intergenic | 50.0% | |
| !! | TTTTTGGTCCATGACCCGCT+GGG | - | chr3.1:73607982-73608001 | MS.gene057115:intron | 50.0% |
| GGTGGCCTCACAGAAAACTG+AGG | - | chr3.1:73607819-73607838 | MS.gene057115:intron | 55.0% | |
| !!! | GTTTTTGGTCCATGACCCGC+TGG | - | chr3.1:73607981-73608000 | MS.gene057115:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 73607313 | 73608741 | 73607313 | ID=MS.gene057115 |
| chr3.1 | mRNA | 73607313 | 73608741 | 73607313 | ID=MS.gene057115.t1;Parent=MS.gene057115 |
| chr3.1 | exon | 73608183 | 73608741 | 73608183 | ID=MS.gene057115.t1.exon1;Parent=MS.gene057115.t1 |
| chr3.1 | CDS | 73608183 | 73608741 | 73608183 | ID=cds.MS.gene057115.t1;Parent=MS.gene057115.t1 |
| chr3.1 | exon | 73607313 | 73607471 | 73607313 | ID=MS.gene057115.t1.exon2;Parent=MS.gene057115.t1 |
| chr3.1 | CDS | 73607313 | 73607471 | 73607313 | ID=cds.MS.gene057115.t1;Parent=MS.gene057115.t1 |
| Gene Sequence |
| Protein sequence |
