Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057309.t1 | PNX94489.1 | 100 | 65 | 0 | 0 | 1 | 65 | 190 | 254 | 1.20E-27 | 131.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057309.t1 | O65679 | 64.6 | 65 | 23 | 0 | 1 | 65 | 187 | 251 | 6.7e-19 | 94.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057309.t1 | A0A2K3MUW4 | 100.0 | 65 | 0 | 0 | 1 | 65 | 190 | 254 | 8.7e-28 | 131.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057309 | MS.gene057310 | 0.833905 | 4.08E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057309.t1 | MTR_4g070360 | 96.923 | 65 | 2 | 0 | 1 | 65 | 190 | 254 | 7.54e-40 | 132 |
| MS.gene057309.t1 | MTR_3g116840 | 59.375 | 64 | 26 | 0 | 2 | 65 | 194 | 257 | 7.49e-24 | 90.9 |
| MS.gene057309.t1 | MTR_3g111990 | 57.812 | 64 | 27 | 0 | 2 | 65 | 190 | 253 | 3.55e-23 | 89.0 |
| MS.gene057309.t1 | MTR_5g020740 | 58.730 | 63 | 26 | 0 | 3 | 65 | 192 | 254 | 5.03e-22 | 85.9 |
| MS.gene057309.t1 | MTR_3g111980 | 58.730 | 63 | 26 | 0 | 2 | 64 | 192 | 254 | 1.41e-21 | 84.7 |
| MS.gene057309.t1 | MTR_5g027290 | 52.308 | 65 | 31 | 0 | 1 | 65 | 194 | 258 | 4.34e-20 | 80.9 |
| MS.gene057309.t1 | MTR_5g027240 | 52.308 | 65 | 31 | 0 | 1 | 65 | 194 | 258 | 4.62e-20 | 80.9 |
| MS.gene057309.t1 | MTR_4g070370 | 57.377 | 61 | 26 | 0 | 5 | 65 | 196 | 256 | 7.63e-19 | 77.8 |
| MS.gene057309.t1 | MTR_4g070340 | 52.381 | 63 | 30 | 0 | 3 | 65 | 188 | 250 | 1.74e-18 | 76.6 |
| MS.gene057309.t1 | MTR_4g070340 | 52.381 | 63 | 30 | 0 | 3 | 65 | 193 | 255 | 1.80e-18 | 76.6 |
| MS.gene057309.t1 | MTR_4g070340 | 52.381 | 63 | 30 | 0 | 3 | 65 | 144 | 206 | 2.97e-18 | 75.5 |
| MS.gene057309.t1 | MTR_8g071130 | 52.308 | 65 | 31 | 0 | 1 | 65 | 194 | 258 | 1.22e-17 | 74.3 |
| MS.gene057309.t1 | MTR_5g020760 | 49.231 | 65 | 33 | 0 | 1 | 65 | 200 | 264 | 2.89e-17 | 72.8 |
| MS.gene057309.t1 | MTR_5g020760 | 49.231 | 65 | 33 | 0 | 1 | 65 | 200 | 264 | 3.51e-17 | 73.2 |
| MS.gene057309.t1 | MTR_5g020800 | 49.231 | 65 | 33 | 0 | 1 | 65 | 200 | 264 | 4.48e-17 | 72.8 |
| MS.gene057309.t1 | MTR_1g093720 | 49.123 | 57 | 29 | 0 | 5 | 61 | 203 | 259 | 3.82e-14 | 65.1 |
| MS.gene057309.t1 | MTR_3g008100 | 43.333 | 60 | 34 | 0 | 3 | 62 | 182 | 241 | 2.93e-13 | 62.4 |
| MS.gene057309.t1 | MTR_3g008100 | 43.333 | 60 | 34 | 0 | 3 | 62 | 189 | 248 | 3.26e-13 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057309.t1 | AT4G34540 | 64.615 | 65 | 23 | 0 | 1 | 65 | 187 | 251 | 1.69e-25 | 95.1 |
| MS.gene057309.t1 | AT4G39230 | 63.492 | 63 | 23 | 0 | 2 | 64 | 190 | 252 | 4.39e-25 | 94.4 |
| MS.gene057309.t1 | AT1G75280 | 59.375 | 64 | 26 | 0 | 2 | 65 | 193 | 256 | 4.61e-23 | 89.0 |
| MS.gene057309.t1 | AT1G75290 | 56.250 | 64 | 28 | 0 | 2 | 65 | 220 | 283 | 1.86e-22 | 87.8 |
| MS.gene057309.t1 | AT1G75290 | 56.250 | 64 | 28 | 0 | 2 | 65 | 193 | 256 | 2.47e-22 | 87.0 |
| MS.gene057309.t1 | AT1G75300 | 59.375 | 64 | 26 | 0 | 2 | 65 | 207 | 270 | 2.64e-22 | 87.0 |
| MS.gene057309.t1 | AT1G19540 | 58.730 | 63 | 26 | 0 | 3 | 65 | 193 | 255 | 9.68e-22 | 84.7 |
| MS.gene057309.t1 | AT1G19540 | 58.730 | 63 | 26 | 0 | 3 | 65 | 193 | 255 | 1.62e-21 | 84.7 |
| MS.gene057309.t1 | AT1G32100 | 45.161 | 62 | 34 | 0 | 3 | 64 | 202 | 263 | 8.66e-14 | 63.9 |
| MS.gene057309.t1 | AT4G13660 | 43.077 | 65 | 37 | 0 | 1 | 65 | 200 | 264 | 2.13e-13 | 62.8 |
| MS.gene057309.t1 | AT4G13660 | 43.077 | 65 | 37 | 0 | 1 | 65 | 200 | 264 | 2.13e-13 | 62.8 |
Find 13 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGATATGGGAAACTAAAAT+AGG | 0.244528 | 4.2:+36590763 | MS.gene057309:CDS |
| TTCAAAGAACAAACATTTCC+TGG | 0.311587 | 4.2:-36590730 | None:intergenic |
| TGAATGAGCTGGTTGAGATA+TGG | 0.375861 | 4.2:+36590749 | MS.gene057309:CDS |
| GAATGAGCTGGTTGAGATAT+GGG | 0.421530 | 4.2:+36590750 | MS.gene057309:CDS |
| AGATATGGGAAACTAAAATA+GGG | 0.489971 | 4.2:+36590764 | MS.gene057309:CDS |
| CAAAACTTTATTCAACGTGC+GGG | 0.536330 | 4.2:-36590695 | None:intergenic |
| TGTTTGTTCTTTGAATGAGC+TGG | 0.551475 | 4.2:+36590738 | MS.gene057309:CDS |
| GAGCTGCTTGAGAAAATCAA+AGG | 0.590625 | 4.2:+36590820 | MS.gene057309:CDS |
| ACAAAACTTTATTCAACGTG+CGG | 0.614674 | 4.2:-36590696 | None:intergenic |
| AAATAGGGAAGAAACTTGAG+AGG | 0.666892 | 4.2:+36590779 | MS.gene057309:CDS |
| AAAACTTTATTCAACGTGCG+GGG | 0.672201 | 4.2:-36590694 | None:intergenic |
| TGCTGCTTTCACTATCAATG+CGG | 0.675047 | 4.2:+36590663 | MS.gene057309:CDS |
| AAAGAACAAACATTTCCTGG+AGG | 0.691831 | 4.2:-36590727 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGATATGGGAAACTAAAATA+GGG | + | chr4.2:36590764-36590783 | MS.gene057309:CDS | 25.0% |
| ACAAAACTTTATTCAACGTG+CGG | - | chr4.2:36590699-36590718 | None:intergenic | 30.0% | |
| GAGATATGGGAAACTAAAAT+AGG | + | chr4.2:36590763-36590782 | MS.gene057309:CDS | 30.0% | |
| TTCAAAGAACAAACATTTCC+TGG | - | chr4.2:36590733-36590752 | None:intergenic | 30.0% | |
| AAAACTTTATTCAACGTGCG+GGG | - | chr4.2:36590697-36590716 | None:intergenic | 35.0% | |
| AAAGAACAAACATTTCCTGG+AGG | - | chr4.2:36590730-36590749 | None:intergenic | 35.0% | |
| AAATAGGGAAGAAACTTGAG+AGG | + | chr4.2:36590779-36590798 | MS.gene057309:CDS | 35.0% | |
| CAAAACTTTATTCAACGTGC+GGG | - | chr4.2:36590698-36590717 | None:intergenic | 35.0% | |
| TGTTTGTTCTTTGAATGAGC+TGG | + | chr4.2:36590738-36590757 | MS.gene057309:CDS | 35.0% | |
| GAATGAGCTGGTTGAGATAT+GGG | + | chr4.2:36590750-36590769 | MS.gene057309:CDS | 40.0% | |
| TGAATGAGCTGGTTGAGATA+TGG | + | chr4.2:36590749-36590768 | MS.gene057309:CDS | 40.0% | |
| !! | TGCTGCTTTCACTATCAATG+CGG | + | chr4.2:36590663-36590682 | MS.gene057309:CDS | 40.0% |
| ! | GTTTTGTACTTGAGACCTCC+AGG | + | chr4.2:36590712-36590731 | MS.gene057309:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 36590646 | 36590841 | 36590646 | ID=MS.gene057309 |
| chr4.2 | mRNA | 36590646 | 36590841 | 36590646 | ID=MS.gene057309.t1;Parent=MS.gene057309 |
| chr4.2 | exon | 36590646 | 36590841 | 36590646 | ID=MS.gene057309.t1.exon1;Parent=MS.gene057309.t1 |
| chr4.2 | CDS | 36590646 | 36590841 | 36590646 | ID=cds.MS.gene057309.t1;Parent=MS.gene057309.t1 |
| Gene Sequence |
| Protein sequence |
