Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057310.t1 | PNX94489.1 | 96.4 | 194 | 7 | 0 | 1 | 194 | 61 | 254 | 2.40E-100 | 374.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057310.t1 | O65679 | 64.4 | 194 | 67 | 1 | 1 | 194 | 60 | 251 | 5.6e-69 | 261.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057310.t1 | A0A2K3MUW4 | 96.4 | 194 | 7 | 0 | 1 | 194 | 61 | 254 | 1.8e-100 | 374.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050529 | MS.gene057310 | 0.809159 | 2.17E-50 | -1.69E-46 |
| MS.gene05105 | MS.gene057310 | 0.801173 | 1.02E-48 | -1.69E-46 |
| MS.gene052134 | MS.gene057310 | 0.826241 | 3.02E-54 | -1.69E-46 |
| MS.gene052135 | MS.gene057310 | 0.816888 | 4.38E-52 | -1.69E-46 |
| MS.gene052253 | MS.gene057310 | 0.808151 | 3.57E-50 | -1.69E-46 |
| MS.gene052481 | MS.gene057310 | 0.803289 | 3.74E-49 | -1.69E-46 |
| MS.gene052505 | MS.gene057310 | 0.806533 | 7.85E-50 | -1.69E-46 |
| MS.gene053103 | MS.gene057310 | 0.821168 | 4.66E-53 | -1.69E-46 |
| MS.gene054918 | MS.gene057310 | 0.822975 | 1.78E-53 | -1.69E-46 |
| MS.gene055116 | MS.gene057310 | 0.807346 | 5.29E-50 | -1.69E-46 |
| MS.gene055289 | MS.gene057310 | 0.825904 | 3.64E-54 | -1.69E-46 |
| MS.gene056330 | MS.gene057310 | -0.802446 | 5.59E-49 | -1.69E-46 |
| MS.gene056511 | MS.gene057310 | 0.811195 | 7.91E-51 | -1.69E-46 |
| MS.gene057146 | MS.gene057310 | 0.824775 | 6.72E-54 | -1.69E-46 |
| MS.gene057309 | MS.gene057310 | 0.833905 | 4.08E-56 | -1.69E-46 |
| MS.gene057310 | MS.gene058233 | 0.803463 | 3.44E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene058376 | 0.803233 | 3.84E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene059536 | 0.807918 | 4.00E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene059688 | 0.820404 | 6.98E-53 | -1.69E-46 |
| MS.gene057310 | MS.gene060403 | 0.810371 | 1.19E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene061606 | 0.807744 | 4.35E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene061838 | -0.8007 | 1.27E-48 | -1.69E-46 |
| MS.gene057310 | MS.gene062219 | 0.819149 | 1.35E-52 | -1.69E-46 |
| MS.gene057310 | MS.gene062227 | 0.81972 | 1.00E-52 | -1.69E-46 |
| MS.gene057310 | MS.gene063114 | 0.801276 | 9.72E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene064168 | 0.801246 | 9.86E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene064203 | 0.800207 | 1.61E-48 | -1.69E-46 |
| MS.gene057310 | MS.gene064789 | 0.827392 | 1.60E-54 | -1.69E-46 |
| MS.gene057310 | MS.gene064875 | 0.830606 | 2.67E-55 | -1.69E-46 |
| MS.gene057310 | MS.gene065841 | 0.81425 | 1.69E-51 | -1.69E-46 |
| MS.gene057310 | MS.gene067501 | 0.811346 | 7.33E-51 | -1.69E-46 |
| MS.gene057310 | MS.gene067502 | 0.808481 | 3.03E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene067522 | 0.808568 | 2.91E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene067523 | 0.802312 | 5.96E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene067741 | 0.810409 | 1.17E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene068612 | 0.803643 | 3.16E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene069086 | 0.801933 | 7.13E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene06973 | 0.80145 | 8.95E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene069859 | 0.813943 | 1.98E-51 | -1.69E-46 |
| MS.gene057310 | MS.gene069976 | 0.829479 | 5.03E-55 | -1.69E-46 |
| MS.gene057310 | MS.gene070108 | 0.808082 | 3.69E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene070206 | 0.805927 | 1.05E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene070207 | 0.818646 | 1.76E-52 | -1.69E-46 |
| MS.gene057310 | MS.gene070950 | 0.81085 | 9.39E-51 | -1.69E-46 |
| MS.gene057310 | MS.gene07114 | 0.809544 | 1.80E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene072430 | 0.837718 | 4.41E-57 | -1.69E-46 |
| MS.gene057310 | MS.gene072757 | 0.801729 | 7.85E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene074597 | 0.802107 | 6.56E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene07625 | 0.806107 | 9.66E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene07862 | 0.835122 | 2.02E-56 | -1.69E-46 |
| MS.gene057310 | MS.gene08513 | 0.809378 | 1.95E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene09725 | 0.800651 | 1.30E-48 | -1.69E-46 |
| MS.gene057310 | MS.gene09727 | 0.803351 | 3.63E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene09923 | 0.800752 | 1.24E-48 | -1.69E-46 |
| MS.gene057310 | MS.gene20225 | 0.806969 | 6.35E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene21534 | 0.800953 | 1.13E-48 | -1.69E-46 |
| MS.gene057310 | MS.gene22206 | 0.804625 | 1.97E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene22209 | 0.804171 | 2.46E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene22846 | 0.829588 | 4.73E-55 | -1.69E-46 |
| MS.gene057310 | MS.gene22985 | 0.842728 | 2.17E-58 | -1.69E-46 |
| MS.gene057310 | MS.gene25171 | 0.80142 | 9.08E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene26265 | 0.804234 | 2.38E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene27498 | 0.815381 | 9.52E-52 | -1.69E-46 |
| MS.gene057310 | MS.gene28441 | 0.804974 | 1.67E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene29105 | 0.803758 | 2.99E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene30306 | 0.849705 | 2.72E-60 | -1.69E-46 |
| MS.gene057310 | MS.gene30834 | 0.821777 | 3.37E-53 | -1.69E-46 |
| MS.gene057310 | MS.gene31671 | 0.80609 | 9.74E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene33571 | 0.831159 | 1.95E-55 | -1.69E-46 |
| MS.gene057310 | MS.gene33658 | 0.818562 | 1.84E-52 | -1.69E-46 |
| MS.gene057310 | MS.gene34319 | 0.803713 | 3.06E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene36476 | 0.811057 | 8.47E-51 | -1.69E-46 |
| MS.gene057310 | MS.gene38351 | 0.80593 | 1.05E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene38588 | 0.803282 | 3.76E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene38966 | 0.81936 | 1.21E-52 | -1.69E-46 |
| MS.gene057310 | MS.gene40205 | 0.81023 | 1.28E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene41414 | -0.801081 | 1.07E-48 | -1.69E-46 |
| MS.gene057310 | MS.gene42798 | 0.809873 | 1.53E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene43407 | 0.809056 | 2.28E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene43413 | 0.824288 | 8.75E-54 | -1.69E-46 |
| MS.gene057310 | MS.gene46942 | 0.811742 | 6.01E-51 | -1.69E-46 |
| MS.gene057310 | MS.gene46956 | 0.823566 | 1.29E-53 | -1.69E-46 |
| MS.gene057310 | MS.gene47389 | 0.823387 | 1.42E-53 | -1.69E-46 |
| MS.gene057310 | MS.gene47758 | 0.8228 | 1.95E-53 | -1.69E-46 |
| MS.gene057310 | MS.gene47869 | 0.809854 | 1.54E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene48278 | 0.805985 | 1.02E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene48355 | 0.800561 | 1.36E-48 | -1.69E-46 |
| MS.gene057310 | MS.gene48593 | 0.801999 | 6.91E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene48662 | 0.803344 | 3.65E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene49872 | 0.807948 | 3.94E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene49873 | 0.806922 | 6.50E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene51540 | 0.825622 | 4.24E-54 | -1.69E-46 |
| MS.gene057310 | MS.gene53150 | 0.808859 | 2.52E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene53332 | 0.818266 | 2.14E-52 | -1.69E-46 |
| MS.gene057310 | MS.gene55270 | 0.832695 | 8.16E-56 | -1.69E-46 |
| MS.gene057310 | MS.gene56177 | 0.803286 | 3.75E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene56216 | 0.803916 | 2.77E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene56713 | 0.804132 | 2.50E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene57561 | 0.827282 | 1.70E-54 | -1.69E-46 |
| MS.gene057310 | MS.gene58817 | 0.809717 | 1.65E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene59353 | 0.803034 | 4.23E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene59950 | 0.807418 | 5.10E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene60021 | 0.809237 | 2.09E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene60395 | 0.804354 | 2.25E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene60911 | 0.809866 | 1.53E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene61157 | 0.801075 | 1.07E-48 | -1.69E-46 |
| MS.gene057310 | MS.gene61579 | 0.829286 | 5.61E-55 | -1.69E-46 |
| MS.gene057310 | MS.gene62420 | 0.801201 | 1.01E-48 | -1.69E-46 |
| MS.gene057310 | MS.gene63538 | 0.810435 | 1.15E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene63913 | -0.803081 | 4.13E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene63955 | 0.818671 | 1.74E-52 | -1.69E-46 |
| MS.gene057310 | MS.gene65641 | 0.830891 | 2.27E-55 | -1.69E-46 |
| MS.gene057310 | MS.gene66702 | 0.832007 | 1.21E-55 | -1.69E-46 |
| MS.gene057310 | MS.gene66835 | 0.826218 | 3.06E-54 | -1.69E-46 |
| MS.gene057310 | MS.gene66847 | 0.804021 | 2.64E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene66972 | 0.815305 | 9.90E-52 | -1.69E-46 |
| MS.gene057310 | MS.gene69489 | 0.819758 | 9.81E-53 | -1.69E-46 |
| MS.gene057310 | MS.gene70267 | 0.817033 | 4.07E-52 | -1.69E-46 |
| MS.gene057310 | MS.gene71006 | 0.822817 | 1.93E-53 | -1.69E-46 |
| MS.gene057310 | MS.gene74225 | 0.805621 | 1.22E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene75157 | 0.806612 | 7.56E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene75201 | -0.807106 | 5.94E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene77433 | 0.806526 | 7.88E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene77925 | 0.805135 | 1.54E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene78321 | 0.815015 | 1.15E-51 | -1.69E-46 |
| MS.gene057310 | MS.gene79179 | 0.802795 | 4.74E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene82561 | 0.81496 | 1.18E-51 | -1.69E-46 |
| MS.gene057310 | MS.gene82562 | 0.817086 | 3.96E-52 | -1.69E-46 |
| MS.gene057310 | MS.gene83508 | 0.809826 | 1.56E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene85703 | 0.808778 | 2.62E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene85919 | 0.835917 | 1.27E-56 | -1.69E-46 |
| MS.gene057310 | MS.gene87043 | -0.806352 | 8.58E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene87268 | 0.815689 | 8.13E-52 | -1.69E-46 |
| MS.gene057310 | MS.gene87271 | 0.807821 | 4.19E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene87280 | 0.80091 | 1.15E-48 | -1.69E-46 |
| MS.gene057310 | MS.gene87687 | 0.827942 | 1.18E-54 | -1.69E-46 |
| MS.gene057310 | MS.gene90287 | 0.809469 | 1.86E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene90538 | 0.813637 | 2.31E-51 | -1.69E-46 |
| MS.gene057310 | MS.gene90772 | 0.842745 | 2.14E-58 | -1.69E-46 |
| MS.gene057310 | MS.gene91679 | 0.855371 | 6.60E-62 | -1.69E-46 |
| MS.gene057310 | MS.gene92714 | 0.809047 | 2.29E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene92773 | 0.819948 | 8.88E-53 | -1.69E-46 |
| MS.gene057310 | MS.gene93035 | 0.808507 | 2.99E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene95112 | 0.816417 | 5.59E-52 | -1.69E-46 |
| MS.gene057310 | MS.gene95702 | 0.816027 | 6.83E-52 | -1.69E-46 |
| MS.gene057310 | MS.gene95748 | 0.822139 | 2.78E-53 | -1.69E-46 |
| MS.gene057310 | MS.gene95885 | 0.821573 | 3.76E-53 | -1.69E-46 |
| MS.gene057310 | MS.gene96371 | 0.803848 | 2.87E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene97081 | 0.809918 | 1.49E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene99622 | 0.802539 | 5.35E-49 | -1.69E-46 |
| MS.gene057310 | MS.gene99819 | 0.806072 | 9.82E-50 | -1.69E-46 |
| MS.gene057310 | MS.gene99854 | 0.815252 | 1.02E-51 | -1.69E-46 |
| MS.gene057310 | MS.gene99863 | 0.809371 | 1.96E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057310.t1 | MTR_4g070360 | 97.423 | 194 | 5 | 0 | 1 | 194 | 61 | 254 | 7.22e-138 | 387 |
| MS.gene057310.t1 | MTR_3g116840 | 53.093 | 194 | 89 | 1 | 1 | 194 | 66 | 257 | 3.89e-70 | 216 |
| MS.gene057310.t1 | MTR_3g111990 | 51.546 | 194 | 92 | 1 | 1 | 194 | 62 | 253 | 1.16e-68 | 212 |
| MS.gene057310.t1 | MTR_5g020740 | 52.577 | 194 | 90 | 1 | 1 | 194 | 63 | 254 | 7.88e-67 | 207 |
| MS.gene057310.t1 | MTR_3g111980 | 50.769 | 195 | 92 | 2 | 1 | 193 | 62 | 254 | 8.23e-64 | 199 |
| MS.gene057310.t1 | MTR_5g027240 | 47.739 | 199 | 98 | 2 | 1 | 194 | 61 | 258 | 3.33e-58 | 185 |
| MS.gene057310.t1 | MTR_4g070370 | 48.469 | 196 | 95 | 3 | 1 | 194 | 65 | 256 | 4.82e-58 | 185 |
| MS.gene057310.t1 | MTR_5g027290 | 47.739 | 199 | 98 | 2 | 1 | 194 | 61 | 258 | 5.13e-58 | 184 |
| MS.gene057310.t1 | MTR_5g020760 | 44.330 | 194 | 106 | 1 | 1 | 194 | 73 | 264 | 1.83e-56 | 179 |
| MS.gene057310.t1 | MTR_5g020760 | 44.330 | 194 | 106 | 1 | 1 | 194 | 73 | 264 | 5.57e-56 | 179 |
| MS.gene057310.t1 | MTR_5g020800 | 44.330 | 194 | 106 | 1 | 1 | 194 | 73 | 264 | 7.06e-56 | 179 |
| MS.gene057310.t1 | MTR_8g071130 | 46.465 | 198 | 100 | 2 | 2 | 194 | 62 | 258 | 3.14e-55 | 177 |
| MS.gene057310.t1 | MTR_4g070340 | 42.784 | 194 | 109 | 1 | 1 | 194 | 15 | 206 | 4.16e-53 | 171 |
| MS.gene057310.t1 | MTR_4g070340 | 42.784 | 194 | 109 | 1 | 1 | 194 | 64 | 255 | 1.86e-52 | 171 |
| MS.gene057310.t1 | MTR_4g070340 | 41.237 | 194 | 107 | 2 | 1 | 194 | 64 | 250 | 1.27e-47 | 158 |
| MS.gene057310.t1 | MTR_1g093720 | 40.571 | 175 | 102 | 1 | 16 | 190 | 87 | 259 | 3.11e-42 | 145 |
| MS.gene057310.t1 | MTR_3g008100 | 38.298 | 188 | 108 | 3 | 7 | 191 | 59 | 241 | 3.71e-38 | 133 |
| MS.gene057310.t1 | MTR_3g008100 | 37.696 | 191 | 113 | 3 | 1 | 191 | 64 | 248 | 4.52e-38 | 133 |
| MS.gene057310.t1 | MTR_3g008140 | 36.649 | 191 | 115 | 3 | 1 | 191 | 64 | 248 | 1.15e-36 | 130 |
| MS.gene057310.t1 | MTR_3g007970 | 36.126 | 191 | 116 | 3 | 1 | 191 | 64 | 248 | 2.31e-35 | 126 |
| MS.gene057310.t1 | MTR_3g008100 | 35.156 | 128 | 77 | 3 | 1 | 128 | 64 | 185 | 5.07e-16 | 73.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057310.t1 | AT4G34540 | 64.433 | 194 | 67 | 1 | 1 | 194 | 60 | 251 | 4.75e-92 | 271 |
| MS.gene057310.t1 | AT4G39230 | 53.886 | 193 | 87 | 1 | 1 | 193 | 62 | 252 | 4.94e-72 | 220 |
| MS.gene057310.t1 | AT1G75280 | 53.571 | 196 | 87 | 2 | 1 | 194 | 63 | 256 | 4.38e-69 | 213 |
| MS.gene057310.t1 | AT1G75290 | 51.531 | 196 | 91 | 2 | 1 | 194 | 63 | 256 | 6.88e-69 | 213 |
| MS.gene057310.t1 | AT1G75290 | 51.531 | 196 | 91 | 2 | 1 | 194 | 90 | 283 | 1.37e-68 | 213 |
| MS.gene057310.t1 | AT1G75300 | 46.190 | 210 | 95 | 3 | 1 | 194 | 63 | 270 | 7.62e-61 | 192 |
| MS.gene057310.t1 | AT1G19540 | 47.980 | 198 | 97 | 3 | 1 | 194 | 60 | 255 | 3.06e-57 | 181 |
| MS.gene057310.t1 | AT1G19540 | 47.980 | 198 | 97 | 3 | 1 | 194 | 60 | 255 | 1.39e-56 | 181 |
| MS.gene057310.t1 | AT1G32100 | 44.221 | 199 | 105 | 2 | 1 | 194 | 67 | 264 | 4.83e-51 | 167 |
| MS.gene057310.t1 | AT4G13660 | 41.206 | 199 | 111 | 2 | 1 | 194 | 67 | 264 | 1.57e-46 | 155 |
| MS.gene057310.t1 | AT4G13660 | 41.206 | 199 | 111 | 2 | 1 | 194 | 67 | 264 | 1.57e-46 | 155 |
Find 40 sgRNAs with CRISPR-Local
Find 116 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGATATGGGAAACTAAAAT+AGG | 0.244528 | 4.3:+35163542 | MS.gene057310:CDS |
| AGGGACAAAGTCACTATATT+TGG | 0.279239 | 4.3:+35163299 | MS.gene057310:CDS |
| AGGTTCATCCCATCTGAATT+TGG | 0.279876 | 4.3:+35163095 | MS.gene057310:CDS |
| TTCAAAGAACAAACATTTCC+TGG | 0.311587 | 4.3:-35163509 | None:intergenic |
| TTGCAGGAGATGAAAGTATA+TGG | 0.349566 | 4.3:-35163218 | None:intergenic |
| TGAATGAGCTGGTTGAGATA+TGG | 0.375861 | 4.3:+35163528 | MS.gene057310:CDS |
| TACAACAAAAGCTACTCATC+AGG | 0.411527 | 4.3:+35161909 | MS.gene057310:CDS |
| GAATGAGCTGGTTGAGATAT+GGG | 0.421530 | 4.3:+35163529 | MS.gene057310:CDS |
| CTTCCTTCTCTTGTCCAACC+AGG | 0.425019 | 4.3:+35163260 | MS.gene057310:CDS |
| CAAACAACTTGGTTCTATCA+AGG | 0.437457 | 4.3:+35161937 | MS.gene057310:CDS |
| CAGGATTAAAAGCTCCTCCT+AGG | 0.463730 | 4.3:+35163279 | MS.gene057310:CDS |
| AATTGTAGGTGTATTTATGC+AGG | 0.470701 | 4.3:+35163409 | MS.gene057310:intron |
| AGATATGGGAAACTAAAATA+GGG | 0.489971 | 4.3:+35163543 | MS.gene057310:CDS |
| TTTGGTGACGGCAATACAAA+AGG | 0.514372 | 4.3:+35163317 | MS.gene057310:CDS |
| AATCCTGGTTGGACAAGAGA+AGG | 0.534523 | 4.3:-35163263 | None:intergenic |
| CAAAACTTTATTCAACGTGC+GGG | 0.536330 | 4.3:-35163474 | None:intergenic |
| AATTGAAATTAGGCAACTTG+TGG | 0.541470 | 4.3:+35163181 | MS.gene057310:CDS |
| AAGTTCACATACTTTAGCCT+TGG | 0.542851 | 4.3:-35163129 | None:intergenic |
| ATCAGGGTTATCAAACAACT+TGG | 0.542961 | 4.3:+35161926 | MS.gene057310:CDS |
| TGTTTGTTCTTTGAATGAGC+TGG | 0.551475 | 4.3:+35163517 | MS.gene057310:CDS |
| GGATCTGAGCCAAATTCAGA+TGG | 0.556972 | 4.3:-35163104 | None:intergenic |
| GGCTAAAGTATGTGAACTTG+AGG | 0.561289 | 4.3:+35163133 | MS.gene057310:CDS |
| TCACATACTTTAGCCTTGGT+AGG | 0.568317 | 4.3:-35163125 | None:intergenic |
| AGGATTAAAAGCTCCTCCTA+GGG | 0.569921 | 4.3:+35163280 | MS.gene057310:CDS |
| GAGCTGCTTGAGAAAATCAA+AGG | 0.590625 | 4.3:+35163599 | MS.gene057310:CDS |
| AAAGTATGTGAACTTGAGGA+TGG | 0.599062 | 4.3:+35163137 | MS.gene057310:CDS |
| AGGCAACTTGTGGAAGCTGA+AGG | 0.602341 | 4.3:+35163191 | MS.gene057310:CDS |
| ACAAAACTTTATTCAACGTG+CGG | 0.614674 | 4.3:-35163475 | None:intergenic |
| AATATAGTGACTTTGTCCCT+AGG | 0.615843 | 4.3:-35163296 | None:intergenic |
| ACAACAAAAGCTACTCATCA+GGG | 0.618980 | 4.3:+35161910 | MS.gene057310:CDS |
| AGATGAAGCAAGCCTTGTTG+AGG | 0.626473 | 4.3:+35161835 | MS.gene057310:CDS |
| CTAACTTCACAGCCTCAACA+AGG | 0.643328 | 4.3:-35161847 | None:intergenic |
| AAAGTCACTATATTTGGTGA+CGG | 0.648569 | 4.3:+35163305 | MS.gene057310:CDS |
| ATAGTGACTTTGTCCCTAGG+AGG | 0.649958 | 4.3:-35163293 | None:intergenic |
| GATCTGAGCCAAATTCAGAT+GGG | 0.661245 | 4.3:-35163103 | None:intergenic |
| AAATAGGGAAGAAACTTGAG+AGG | 0.666892 | 4.3:+35163558 | MS.gene057310:CDS |
| AAAACTTTATTCAACGTGCG+GGG | 0.672201 | 4.3:-35163473 | None:intergenic |
| TGCTGCTTTCACTATCAATG+CGG | 0.675047 | 4.3:+35163442 | MS.gene057310:CDS |
| AAAGAACAAACATTTCCTGG+AGG | 0.691831 | 4.3:-35163506 | None:intergenic |
| ATTTGGCTCAGATCCTACCA+AGG | 0.716779 | 4.3:+35163112 | MS.gene057310:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCAATTTATAGAAATATAAT+AGG | - | chr4.3:35162881-35162900 | None:intergenic | 10.0% |
| !!! | TTATCATATTTTTATAGTTT+AGG | - | chr4.3:35162688-35162707 | None:intergenic | 10.0% |
| !! | ACTAGAATATTAAGTAATTT+AGG | + | chr4.3:35162819-35162838 | MS.gene057310:intron | 15.0% |
| !! | CTAGAATATTAAGTAATTTA+GGG | + | chr4.3:35162820-35162839 | MS.gene057310:intron | 15.0% |
| !! | TATAGTATATACTATCAATT+TGG | - | chr4.3:35162921-35162940 | None:intergenic | 15.0% |
| !!! | CTTTTGTTTATAATAAATTG+AGG | + | chr4.3:35162954-35162973 | MS.gene057310:intron | 15.0% |
| !! | AATAAAAAATTCAGCTCAAA+AGG | + | chr4.3:35162333-35162352 | MS.gene057310:intron | 20.0% |
| !! | ATAAAAAATTCAGCTCAAAA+GGG | + | chr4.3:35162334-35162353 | MS.gene057310:intron | 20.0% |
| !! | CTATAAAAATATGATAAGTC+AGG | + | chr4.3:35162691-35162710 | MS.gene057310:intron | 20.0% |
| !!! | ATTCTAGTTCTTATCTTTAA+AGG | - | chr4.3:35162807-35162826 | None:intergenic | 20.0% |
| !!! | ATTTGTTTGTTAACATTTGT+TGG | + | chr4.3:35163066-35163085 | MS.gene057310:intron | 20.0% |
| !!! | TCCAAATTTTTACTTTAGTT+CGG | + | chr4.3:35162147-35162166 | MS.gene057310:intron | 20.0% |
| ! | AAAATAACAAGTATCAGCTA+TGG | - | chr4.3:35162119-35162138 | None:intergenic | 25.0% |
| ! | AGATATGGGAAACTAAAATA+GGG | + | chr4.3:35163543-35163562 | MS.gene057310:CDS | 25.0% |
| ! | ATATATGCGATATATCCTAA+TGG | - | chr4.3:35162610-35162629 | None:intergenic | 25.0% |
| ! | ATGAACTCTTCAAATTCTTA+TGG | + | chr4.3:35161982-35162001 | MS.gene057310:intron | 25.0% |
| ! | ATGCACCAAAAATTGAAATT+AGG | + | chr4.3:35163171-35163190 | MS.gene057310:CDS | 25.0% |
| ! | GGATTATTAAATGTTCATGT+AGG | - | chr4.3:35162995-35163014 | None:intergenic | 25.0% |
| ! | GTCATTGAAAAATATAAGCA+TGG | - | chr4.3:35162260-35162279 | None:intergenic | 25.0% |
| ! | TAATTTAGGGACTAAGTTTA+TGG | + | chr4.3:35162833-35162852 | MS.gene057310:intron | 25.0% |
| ! | TCTATAAATTGAAGAGTGAT+AGG | + | chr4.3:35162889-35162908 | MS.gene057310:intron | 25.0% |
| !! | AATTTTCATGAAGAAATTGC+AGG | - | chr4.3:35163237-35163256 | None:intergenic | 25.0% |
| !! | AGTTGCCTAATTTCAATTTT+TGG | - | chr4.3:35163179-35163198 | None:intergenic | 25.0% |
| !! | CTTATCTTTAAAGGAATTGA+TGG | - | chr4.3:35162798-35162817 | None:intergenic | 25.0% |
| !! | TATGGACTGAAAATAGTTTA+GGG | + | chr4.3:35162851-35162870 | MS.gene057310:intron | 25.0% |
| !! | TGTGATTTGTGTATTCAAAT+AGG | - | chr4.3:35163016-35163035 | None:intergenic | 25.0% |
| !! | TTATGGACTGAAAATAGTTT+AGG | + | chr4.3:35162850-35162869 | MS.gene057310:intron | 25.0% |
| !!! | ATATACTTTTTCAGCTAACA+AGG | - | chr4.3:35162046-35162065 | None:intergenic | 25.0% |
| !!! | CCAAATTTTTACTTTAGTTC+GGG | + | chr4.3:35162148-35162167 | MS.gene057310:intron | 25.0% |
| !!! | GCTGATACTTGTTATTTTTT+AGG | + | chr4.3:35162121-35162140 | MS.gene057310:intron | 25.0% |
| AAACCATTACCACTTGTTTA+AGG | + | chr4.3:35162508-35162527 | MS.gene057310:intron | 30.0% | |
| AATCATGAATAAGTAAGCTC+TGG | - | chr4.3:35163382-35163401 | None:intergenic | 30.0% | |
| AATTGAAATTAGGCAACTTG+TGG | + | chr4.3:35163181-35163200 | MS.gene057310:CDS | 30.0% | |
| AATTGTAGGTGTATTTATGC+AGG | + | chr4.3:35163409-35163428 | MS.gene057310:intron | 30.0% | |
| ACAAAACTTTATTCAACGTG+CGG | - | chr4.3:35163478-35163497 | None:intergenic | 30.0% | |
| AGAGAAAGAACGATAGTTTA+AGG | - | chr4.3:35162656-35162675 | None:intergenic | 30.0% | |
| AGAGCTTACTTATTCATGAT+TGG | + | chr4.3:35163381-35163400 | MS.gene057310:intron | 30.0% | |
| CCCGAACTAAAGTAAAAATT+TGG | - | chr4.3:35162151-35162170 | None:intergenic | 30.0% | |
| CTATACGAATGCTATATTGT+TGG | - | chr4.3:35162284-35162303 | None:intergenic | 30.0% | |
| GAAGATTAATCACTACCAAA+CGG | - | chr4.3:35162411-35162430 | None:intergenic | 30.0% | |
| GAGATATGGGAAACTAAAAT+AGG | + | chr4.3:35163542-35163561 | MS.gene057310:CDS | 30.0% | |
| TATGATAAGTCAGGACTAAT+CGG | + | chr4.3:35162700-35162719 | MS.gene057310:intron | 30.0% | |
| TTCAAAGAACAAACATTTCC+TGG | - | chr4.3:35163512-35163531 | None:intergenic | 30.0% | |
| ! | AAAGTCACTATATTTGGTGA+CGG | + | chr4.3:35163305-35163324 | MS.gene057310:CDS | 30.0% |
| ! | TAATGGACAACATATTCTAG+CGG | - | chr4.3:35162072-35162091 | None:intergenic | 30.0% |
| ! | TATAAACAAAAGCACACATG+TGG | - | chr4.3:35162946-35162965 | None:intergenic | 30.0% |
| !! | AAGCTCTGGAAAAATGAAAA+TGG | - | chr4.3:35163368-35163387 | None:intergenic | 30.0% |
| !!! | CATGATTGGTTTTCAATTGT+AGG | + | chr4.3:35163395-35163414 | MS.gene057310:intron | 30.0% |
| AAAACTTTATTCAACGTGCG+GGG | - | chr4.3:35163476-35163495 | None:intergenic | 35.0% | |
| AAAGAACAAACATTTCCTGG+AGG | - | chr4.3:35163509-35163528 | None:intergenic | 35.0% | |
| AAAGTATGTGAACTTGAGGA+TGG | + | chr4.3:35163137-35163156 | MS.gene057310:CDS | 35.0% | |
| AAATAGGGAAGAAACTTGAG+AGG | + | chr4.3:35163558-35163577 | MS.gene057310:CDS | 35.0% | |
| AAATTGAAGAGTGATAGGCA+GGG | + | chr4.3:35162894-35162913 | MS.gene057310:intron | 35.0% | |
| AAGTTCACATACTTTAGCCT+TGG | - | chr4.3:35163132-35163151 | None:intergenic | 35.0% | |
| AATATAGTGACTTTGTCCCT+AGG | - | chr4.3:35163299-35163318 | None:intergenic | 35.0% | |
| ACAACAAAAGCTACTCATCA+GGG | + | chr4.3:35161910-35161929 | MS.gene057310:CDS | 35.0% | |
| AGACCTTAAACAAGTGGTAA+TGG | - | chr4.3:35162514-35162533 | None:intergenic | 35.0% | |
| AGCTGCTTGAGAAAATCAAA+GGG | + | chr4.3:35163600-35163619 | MS.gene057310:CDS | 35.0% | |
| AGGGACAAAGTCACTATATT+TGG | + | chr4.3:35163299-35163318 | MS.gene057310:CDS | 35.0% | |
| ATCAGGGTTATCAAACAACT+TGG | + | chr4.3:35161926-35161945 | MS.gene057310:CDS | 35.0% | |
| ATGCGATATATCCTAATGGA+AGG | - | chr4.3:35162606-35162625 | None:intergenic | 35.0% | |
| ATGTGTACCCAATAAGTACT+CGG | - | chr4.3:35162436-35162455 | None:intergenic | 35.0% | |
| CAAAACTTTATTCAACGTGC+GGG | - | chr4.3:35163477-35163496 | None:intergenic | 35.0% | |
| GAAAAGAGACCTTAAACAAG+TGG | - | chr4.3:35162520-35162539 | None:intergenic | 35.0% | |
| GCTAGAATATGTTGTCCATT+AGG | + | chr4.3:35162071-35162090 | MS.gene057310:intron | 35.0% | |
| GGAGAAAACTTTGTTGAGAT+TGG | - | chr4.3:35162469-35162488 | None:intergenic | 35.0% | |
| TAAATTGAAGAGTGATAGGC+AGG | + | chr4.3:35162893-35162912 | MS.gene057310:intron | 35.0% | |
| TAATCACTACCAAACGGTAT+CGG | - | chr4.3:35162405-35162424 | None:intergenic | 35.0% | |
| TACAACAAAAGCTACTCATC+AGG | + | chr4.3:35161909-35161928 | MS.gene057310:CDS | 35.0% | |
| TGCGATATATCCTAATGGAA+GGG | - | chr4.3:35162605-35162624 | None:intergenic | 35.0% | |
| TGTTTGTTCTTTGAATGAGC+TGG | + | chr4.3:35163517-35163536 | MS.gene057310:CDS | 35.0% | |
| ! | CTTACATTTTCCCTTCCATT+AGG | + | chr4.3:35162592-35162611 | MS.gene057310:intron | 35.0% |
| ! | TTAACATTTGTTGGCTACAG+AGG | + | chr4.3:35163075-35163094 | MS.gene057310:intron | 35.0% |
| ! | TTCATGTAGGTTGAGTTTCA+AGG | - | chr4.3:35162982-35163001 | None:intergenic | 35.0% |
| ! | TTGCAGGAGATGAAAGTATA+TGG | - | chr4.3:35163221-35163240 | None:intergenic | 35.0% |
| !! | CAAACAACTTGGTTCTATCA+AGG | + | chr4.3:35161937-35161956 | MS.gene057310:CDS | 35.0% |
| AGGATTAAAAGCTCCTCCTA+GGG | + | chr4.3:35163280-35163299 | MS.gene057310:CDS | 40.0% | |
| AGGTTCATCCCATCTGAATT+TGG | + | chr4.3:35163095-35163114 | MS.gene057310:CDS | 40.0% | |
| ATCTTCCACCGAGTACTTAT+TGG | + | chr4.3:35162425-35162444 | MS.gene057310:intron | 40.0% | |
| CTATGGCCTTCAAGAGATAA+AGG | - | chr4.3:35162102-35162121 | None:intergenic | 40.0% | |
| GAATGAGCTGGTTGAGATAT+GGG | + | chr4.3:35163529-35163548 | MS.gene057310:CDS | 40.0% | |
| GAGATAAAGGATCAGCCTAA+TGG | - | chr4.3:35162089-35162108 | None:intergenic | 40.0% | |
| GAGCTGCTTGAGAAAATCAA+AGG | + | chr4.3:35163599-35163618 | MS.gene057310:CDS | 40.0% | |
| GATCTGAGCCAAATTCAGAT+GGG | - | chr4.3:35163106-35163125 | None:intergenic | 40.0% | |
| GCTGATCCTTTATCTCTTGA+AGG | + | chr4.3:35162093-35162112 | MS.gene057310:intron | 40.0% | |
| GGCTAAAGTATGTGAACTTG+AGG | + | chr4.3:35163133-35163152 | MS.gene057310:CDS | 40.0% | |
| TCTTCCACCGAGTACTTATT+GGG | + | chr4.3:35162426-35162445 | MS.gene057310:intron | 40.0% | |
| TGAATGAGCTGGTTGAGATA+TGG | + | chr4.3:35163528-35163547 | MS.gene057310:CDS | 40.0% | |
| ! | ATGAAGTATCCGATACCGTT+TGG | + | chr4.3:35162393-35162412 | MS.gene057310:intron | 40.0% |
| !! | TCACATACTTTAGCCTTGGT+AGG | - | chr4.3:35163128-35163147 | None:intergenic | 40.0% |
| !! | TGCTGCTTTCACTATCAATG+CGG | + | chr4.3:35163442-35163461 | MS.gene057310:CDS | 40.0% |
| !! | TTTGGTGACGGCAATACAAA+AGG | + | chr4.3:35163317-35163336 | MS.gene057310:CDS | 40.0% |
| AATCCTGGTTGGACAAGAGA+AGG | - | chr4.3:35163266-35163285 | None:intergenic | 45.0% | |
| ATAGTGACTTTGTCCCTAGG+AGG | - | chr4.3:35163296-35163315 | None:intergenic | 45.0% | |
| CAGGATTAAAAGCTCCTCCT+AGG | + | chr4.3:35163279-35163298 | MS.gene057310:CDS | 45.0% | |
| CTAACTTCACAGCCTCAACA+AGG | - | chr4.3:35161850-35161869 | None:intergenic | 45.0% | |
| GGATCTGAGCCAAATTCAGA+TGG | - | chr4.3:35163107-35163126 | None:intergenic | 45.0% | |
| TAACCTAACCAGCATGTGGT+TGG | - | chr4.3:35162550-35162569 | None:intergenic | 45.0% | |
| TGCATAACCTAACCAGCATG+TGG | - | chr4.3:35162554-35162573 | None:intergenic | 45.0% | |
| TGTACCCAATAAGTACTCGG+TGG | - | chr4.3:35162433-35162452 | None:intergenic | 45.0% | |
| ! | ATTTGGCTCAGATCCTACCA+AGG | + | chr4.3:35163112-35163131 | MS.gene057310:CDS | 45.0% |
| ! | CTAGGAGGAGCTTTTAATCC+TGG | - | chr4.3:35163281-35163300 | None:intergenic | 45.0% |
| ! | GAGGAGCTTTTAATCCTGGT+TGG | - | chr4.3:35163277-35163296 | None:intergenic | 45.0% |
| ! | GTTTTGTACTTGAGACCTCC+AGG | + | chr4.3:35163491-35163510 | MS.gene057310:CDS | 45.0% |
| !! | AGATGAAGCAAGCCTTGTTG+AGG | + | chr4.3:35161835-35161854 | MS.gene057310:CDS | 45.0% |
| !! | AGTGGTAATGGTTTGATCCC+CGG | - | chr4.3:35162502-35162521 | None:intergenic | 45.0% |
| !! | ATATATTGTTATAAATATTA+TGG | + | chr4.3:35162368-35162387 | MS.gene057310:intron | 5.0% |
| !! | TATTTATAACAATATATAAA+AGG | - | chr4.3:35162365-35162384 | None:intergenic | 5.0% |
| AGGCAACTTGTGGAAGCTGA+AGG | + | chr4.3:35163191-35163210 | MS.gene057310:CDS | 50.0% | |
| CACTTGAACCAACCACATGC+TGG | + | chr4.3:35162539-35162558 | MS.gene057310:intron | 50.0% | |
| CTTCCTTCTCTTGTCCAACC+AGG | + | chr4.3:35163260-35163279 | MS.gene057310:CDS | 50.0% | |
| ! | GAACCAACCACATGCTGGTT+AGG | + | chr4.3:35162544-35162563 | MS.gene057310:intron | 50.0% |
| ! | GTTTTCTCCATCGAGAGAGC+CGG | + | chr4.3:35162480-35162499 | MS.gene057310:intron | 50.0% |
| ! | TTTTCTCCATCGAGAGAGCC+GGG | + | chr4.3:35162481-35162500 | MS.gene057310:intron | 50.0% |
| TTTCTCCATCGAGAGAGCCG+GGG | + | chr4.3:35162482-35162501 | MS.gene057310:intron | 55.0% | |
| TTGATCCCCGGCTCTCTCGA+TGG | - | chr4.3:35162490-35162509 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 35161824 | 35163620 | 35161824 | ID=MS.gene057310 |
| chr4.3 | mRNA | 35161824 | 35163620 | 35161824 | ID=MS.gene057310.t1;Parent=MS.gene057310 |
| chr4.3 | exon | 35161824 | 35161958 | 35161824 | ID=MS.gene057310.t1.exon1;Parent=MS.gene057310.t1 |
| chr4.3 | CDS | 35161824 | 35161958 | 35161824 | ID=cds.MS.gene057310.t1;Parent=MS.gene057310.t1 |
| chr4.3 | exon | 35163095 | 35163338 | 35163095 | ID=MS.gene057310.t1.exon2;Parent=MS.gene057310.t1 |
| chr4.3 | CDS | 35163095 | 35163338 | 35163095 | ID=cds.MS.gene057310.t1;Parent=MS.gene057310.t1 |
| chr4.3 | exon | 35163417 | 35163620 | 35163417 | ID=MS.gene057310.t1.exon3;Parent=MS.gene057310.t1 |
| chr4.3 | CDS | 35163417 | 35163620 | 35163417 | ID=cds.MS.gene057310.t1;Parent=MS.gene057310.t1 |
| Gene Sequence |
| Protein sequence |
