Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057423.t1 | XP_003595357.1 | 95.3 | 255 | 7 | 3 | 1 | 250 | 1 | 255 | 8.80E-135 | 489.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057423.t1 | Q8GYX2 | 38.7 | 266 | 125 | 10 | 1 | 248 | 1 | 246 | 2.0e-34 | 147.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057423.t1 | G7IIQ7 | 95.3 | 255 | 7 | 3 | 1 | 250 | 1 | 255 | 6.3e-135 | 489.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057423 | MS.gene64494 | 0.810215 | 1.29E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057423.t1 | MTR_2g042970 | 95.294 | 255 | 7 | 3 | 1 | 250 | 1 | 255 | 1.17e-179 | 493 |
| MS.gene057423.t1 | MTR_4g124930 | 55.939 | 261 | 93 | 12 | 1 | 250 | 1 | 250 | 1.11e-79 | 240 |
| MS.gene057423.t1 | MTR_4g124930 | 54.918 | 244 | 89 | 12 | 1 | 237 | 1 | 230 | 1.06e-69 | 214 |
| MS.gene057423.t1 | MTR_3g108290 | 49.367 | 79 | 39 | 1 | 170 | 247 | 342 | 420 | 1.58e-15 | 75.9 |
| MS.gene057423.t1 | MTR_5g013770 | 54.430 | 79 | 25 | 3 | 182 | 249 | 85 | 163 | 7.53e-15 | 70.5 |
| MS.gene057423.t1 | MTR_3g073960 | 59.574 | 47 | 18 | 1 | 181 | 226 | 89 | 135 | 8.11e-13 | 64.7 |
| MS.gene057423.t1 | MTR_8g008840 | 57.407 | 54 | 22 | 1 | 173 | 225 | 29 | 82 | 8.13e-13 | 63.5 |
| MS.gene057423.t1 | MTR_1g103500 | 40.566 | 106 | 50 | 4 | 117 | 221 | 192 | 285 | 1.31e-12 | 66.2 |
| MS.gene057423.t1 | MTR_4g099050 | 61.818 | 55 | 17 | 2 | 182 | 235 | 73 | 124 | 8.65e-12 | 62.0 |
| MS.gene057423.t1 | MTR_1g010140 | 34.783 | 138 | 78 | 5 | 117 | 249 | 213 | 343 | 2.17e-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057423.t1 | AT5G20700 | 38.951 | 267 | 125 | 10 | 1 | 249 | 1 | 247 | 3.55e-43 | 147 |
| MS.gene057423.t1 | AT1G74940 | 35.409 | 257 | 123 | 10 | 1 | 250 | 2 | 222 | 2.96e-34 | 123 |
| MS.gene057423.t1 | AT1G19200 | 33.068 | 251 | 136 | 11 | 6 | 250 | 4 | 228 | 7.24e-21 | 88.2 |
| MS.gene057423.t1 | AT1G19200 | 33.778 | 225 | 118 | 10 | 31 | 250 | 17 | 215 | 6.79e-20 | 85.5 |
| MS.gene057423.t1 | AT1G22160 | 64.706 | 51 | 17 | 1 | 181 | 230 | 77 | 127 | 8.00e-16 | 72.8 |
| MS.gene057423.t1 | AT1G53885 | 51.786 | 56 | 26 | 1 | 170 | 224 | 30 | 85 | 4.15e-15 | 70.1 |
| MS.gene057423.t1 | AT1G53903 | 51.786 | 56 | 26 | 1 | 170 | 224 | 30 | 85 | 4.15e-15 | 70.1 |
| MS.gene057423.t1 | AT1G78020 | 59.184 | 49 | 19 | 1 | 182 | 229 | 89 | 137 | 4.21e-13 | 65.9 |
| MS.gene057423.t1 | AT3G22550 | 28.794 | 257 | 120 | 11 | 8 | 224 | 32 | 265 | 5.26e-13 | 67.4 |
| MS.gene057423.t1 | AT2G44670 | 64.444 | 45 | 15 | 1 | 182 | 225 | 16 | 60 | 4.65e-12 | 61.2 |
| MS.gene057423.t1 | AT5G47060 | 46.835 | 79 | 31 | 3 | 182 | 249 | 97 | 175 | 1.10e-11 | 62.0 |
| MS.gene057423.t1 | AT5G47060 | 46.835 | 79 | 31 | 3 | 182 | 249 | 97 | 175 | 1.10e-11 | 62.0 |
| MS.gene057423.t1 | AT5G65040 | 58.696 | 46 | 18 | 1 | 181 | 225 | 56 | 101 | 1.24e-11 | 60.5 |
| MS.gene057423.t1 | AT4G17670 | 62.500 | 48 | 17 | 1 | 182 | 228 | 76 | 123 | 1.56e-11 | 61.2 |
Find 35 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATAAATCCGATAACGATTT+TGG | 0.086713 | 2.1:-51235898 | MS.gene057423:CDS |
| CTCAATTCAAACGGGCTTCT+AGG | 0.297546 | 2.1:+51235997 | None:intergenic |
| GTCACATAAGTGTAATCTTC+TGG | 0.307242 | 2.1:+51235748 | None:intergenic |
| CAAAATCATCAACAACAAAA+TGG | 0.342676 | 2.1:-51235802 | MS.gene057423:CDS |
| TTTGCAAATGAGATTCTTGT+TGG | 0.361648 | 2.1:-51235775 | MS.gene057423:CDS |
| GAATTTGGTGGTGTTGGTCT+TGG | 0.362778 | 2.1:-51235937 | MS.gene057423:CDS |
| AAAATTATCATCATCATGTT+GGG | 0.363272 | 2.1:-51236108 | None:intergenic |
| TAATCCCAGTTGAAAACATT+TGG | 0.378194 | 2.1:+51235152 | None:intergenic |
| AACATTTGGTCCTTTGTGTA+TGG | 0.411067 | 2.1:+51235166 | None:intergenic |
| CTCGTGCCCAAAATCGTTAT+CGG | 0.417278 | 2.1:+51235891 | None:intergenic |
| ATCTTTGAGCTCAATTCAAA+CGG | 0.420646 | 2.1:+51235988 | None:intergenic |
| TTAGGTGTACAAACATGTTT+CGG | 0.424274 | 2.1:+51235862 | None:intergenic |
| TCTCCTGATCGATTTGACTT+AGG | 0.433913 | 2.1:+51235844 | None:intergenic |
| GAGCTAAAGTTTGTGTTGGT+GGG | 0.449305 | 2.1:+51235602 | None:intergenic |
| AGCTATGAATTTGGTGGTGT+TGG | 0.457246 | 2.1:-51235943 | MS.gene057423:CDS |
| TATGATGGCGGTGAAGAAAG+TGG | 0.470889 | 2.1:-51235685 | MS.gene057423:CDS |
| AAATCCAAGGTTGGATATGA+TGG | 0.475932 | 2.1:+51235571 | None:intergenic |
| TGGTGTTGGTCTTGGAATTG+TGG | 0.476764 | 2.1:-51235929 | MS.gene057423:CDS |
| TCTTTGAGCTCAATTCAAAC+GGG | 0.490170 | 2.1:+51235989 | None:intergenic |
| AGAGAAGTGTAGATCATCAA+TGG | 0.509195 | 2.1:-51235203 | MS.gene057423:CDS |
| TCAAAGATTCAATCACCAAA+AGG | 0.513577 | 2.1:-51235973 | MS.gene057423:CDS |
| TCGTTCAAATCAAATAATGA+TGG | 0.514959 | 2.1:-51235236 | MS.gene057423:CDS |
| TGAGCTAAAGTTTGTGTTGG+TGG | 0.516581 | 2.1:+51235601 | None:intergenic |
| AGCTAAAGTTTGTGTTGGTG+GGG | 0.518025 | 2.1:+51235603 | None:intergenic |
| ACACCTAAGTCAAATCGATC+AGG | 0.521467 | 2.1:-51235847 | MS.gene057423:CDS |
| GCAAAGACATATTCATGTAC+AGG | 0.528815 | 2.1:-51235510 | MS.gene057423:intron |
| ATCTAATAAACCTATCACTA+AGG | 0.533202 | 2.1:-51235713 | MS.gene057423:CDS |
| TCCGATTGTGTCGAAAAGCC+CGG | 0.539258 | 2.1:+51236023 | None:intergenic |
| ATCAAATAATGATGGATGAG+AGG | 0.547584 | 2.1:-51235228 | MS.gene057423:CDS |
| GCTTGAGCTAAAGTTTGTGT+TGG | 0.552900 | 2.1:+51235598 | None:intergenic |
| GAATTGAGAAAATCCAAGGT+TGG | 0.589378 | 2.1:+51235562 | None:intergenic |
| ACATGAATTGAGAAAATCCA+AGG | 0.596600 | 2.1:+51235558 | None:intergenic |
| AAATTATCATCATCATGTTG+GGG | 0.621808 | 2.1:-51236107 | None:intergenic |
| TGAACCATCATATCCAACCT+TGG | 0.622687 | 2.1:-51235575 | MS.gene057423:CDS |
| ATCAAGTTCTCCATACACAA+AGG | 0.691311 | 2.1:-51235176 | MS.gene057423:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTTAGATATATGATTAAT+TGG | - | chr2.1:51235923-51235942 | MS.gene057423:CDS | 10.0% |
| !!! | AAGAATTTTAATTTTAGATT+TGG | + | chr2.1:51235756-51235775 | None:intergenic | 10.0% |
| !!! | AGAATTTTAATTTTAGATTT+GGG | + | chr2.1:51235755-51235774 | None:intergenic | 10.0% |
| !!! | TTAATTGGTAATTATAATTT+TGG | - | chr2.1:51235938-51235957 | MS.gene057423:CDS | 10.0% |
| ! | ATCTAATAAACCTATCACTA+AGG | - | chr2.1:51235521-51235540 | MS.gene057423:CDS | 25.0% |
| ! | CAAAATCATCAACAACAAAA+TGG | - | chr2.1:51235432-51235451 | MS.gene057423:intron | 25.0% |
| ! | GGATTAAAAAGCTATGAATT+TGG | - | chr2.1:51235282-51235301 | MS.gene057423:intron | 25.0% |
| ! | TCATAAAAAACCTTAGTGAT+AGG | + | chr2.1:51235534-51235553 | None:intergenic | 25.0% |
| ! | TCGTTCAAATCAAATAATGA+TGG | - | chr2.1:51235998-51236017 | MS.gene057423:CDS | 25.0% |
| ! | TTAAAAAGCTATGAATTTGG+TGG | - | chr2.1:51235285-51235304 | MS.gene057423:intron | 25.0% |
| !! | ATAAATCCGATAACGATTTT+GGG | - | chr2.1:51235337-51235356 | MS.gene057423:intron | 25.0% |
| !! | TCATAGCTTTTTAATCCTTT+TGG | + | chr2.1:51235279-51235298 | None:intergenic | 25.0% |
| !!! | ATCACTAAGGTTTTTTATGA+TGG | - | chr2.1:51235534-51235553 | MS.gene057423:CDS | 25.0% |
| !!! | ATTATAATTTTGGTGTGTAG+AGG | - | chr2.1:51235948-51235967 | MS.gene057423:CDS | 25.0% |
| !!! | CTAGTAGTTCTGAAAATTTT+CGG | + | chr2.1:51235173-51235192 | None:intergenic | 25.0% |
| !!! | TGTTGTTGATGATTTTGAAT+TGG | + | chr2.1:51235429-51235448 | None:intergenic | 25.0% |
| !!! | TTATAATTTTGGTGTGTAGA+GGG | - | chr2.1:51235949-51235968 | MS.gene057423:CDS | 25.0% |
| !!! | TTTTCAGAACTACTAGTTTT+CGG | - | chr2.1:51235177-51235196 | MS.gene057423:CDS | 25.0% |
| ACATGAATTGAGAAAATCCA+AGG | + | chr2.1:51235679-51235698 | None:intergenic | 30.0% | |
| ATCTTTGAGCTCAATTCAAA+CGG | + | chr2.1:51235249-51235268 | None:intergenic | 30.0% | |
| TAATCCCAGTTGAAAACATT+TGG | + | chr2.1:51236085-51236104 | None:intergenic | 30.0% | |
| TCAAAGATTCAATCACCAAA+AGG | - | chr2.1:51235261-51235280 | MS.gene057423:CDS | 30.0% | |
| TTAGGTGTACAAACATGTTT+CGG | + | chr2.1:51235375-51235394 | None:intergenic | 30.0% | |
| TTGTGCAAAAAGAATCTTCA+TGG | - | chr2.1:51235702-51235721 | MS.gene057423:CDS | 30.0% | |
| TTTGCAAATGAGATTCTTGT+TGG | - | chr2.1:51235459-51235478 | MS.gene057423:intron | 30.0% | |
| ! | AATGTTTTCAACTGGGATTA+TGG | - | chr2.1:51236085-51236104 | MS.gene057423:CDS | 30.0% |
| ! | GATAAATCCGATAACGATTT+TGG | - | chr2.1:51235336-51235355 | MS.gene057423:intron | 30.0% |
| ! | TTTTGAATTGGAATCGTGAT+CGG | + | chr2.1:51235417-51235436 | None:intergenic | 30.0% |
| !! | ATCAAATAATGATGGATGAG+AGG | - | chr2.1:51236006-51236025 | MS.gene057423:CDS | 30.0% |
| !! | TATAATTTTGGTGTGTAGAG+GGG | - | chr2.1:51235950-51235969 | MS.gene057423:CDS | 30.0% |
| !!! | ACTAAGGTTTTTTATGATGG+CGG | - | chr2.1:51235537-51235556 | MS.gene057423:CDS | 30.0% |
| AAATCCAAGGTTGGATATGA+TGG | + | chr2.1:51235666-51235685 | None:intergenic | 35.0% | |
| AACATTTGGTCCTTTGTGTA+TGG | + | chr2.1:51236071-51236090 | None:intergenic | 35.0% | |
| ACCTCAAAAAACAACAACGT+TGG | - | chr2.1:51235594-51235613 | MS.gene057423:CDS | 35.0% | |
| ATCAAGTTCTCCATACACAA+AGG | - | chr2.1:51236058-51236077 | MS.gene057423:CDS | 35.0% | |
| GAATTGAGAAAATCCAAGGT+TGG | + | chr2.1:51235675-51235694 | None:intergenic | 35.0% | |
| GCAAAGACATATTCATGTAC+AGG | - | chr2.1:51235724-51235743 | MS.gene057423:CDS | 35.0% | |
| GTCACATAAGTGTAATCTTC+TGG | + | chr2.1:51235489-51235508 | None:intergenic | 35.0% | |
| TCTTTGAGCTCAATTCAAAC+GGG | + | chr2.1:51235248-51235267 | None:intergenic | 35.0% | |
| ! | AAAATTTTCGGATCAAGTGC+TGG | + | chr2.1:51235161-51235180 | None:intergenic | 35.0% |
| ! | AAATTTTCGGATCAAGTGCT+GGG | + | chr2.1:51235160-51235179 | None:intergenic | 35.0% |
| ! | AAGGACCAAATGTTTTCAAC+TGG | - | chr2.1:51236077-51236096 | MS.gene057423:CDS | 35.0% |
| ! | AGAGAAGTGTAGATCATCAA+TGG | - | chr2.1:51236031-51236050 | MS.gene057423:CDS | 35.0% |
| ! | AGGACCAAATGTTTTCAACT+GGG | - | chr2.1:51236078-51236097 | MS.gene057423:CDS | 35.0% |
| !! | TAGCGACGAAAATTTTGAGA+CGG | + | chr2.1:51235837-51235856 | None:intergenic | 35.0% |
| !!! | ACCAACGTTGTTGTTTTTTG+AGG | + | chr2.1:51235598-51235617 | None:intergenic | 35.0% |
| ACACCTAAGTCAAATCGATC+AGG | - | chr2.1:51235387-51235406 | MS.gene057423:intron | 40.0% | |
| GCTTGAGCTAAAGTTTGTGT+TGG | + | chr2.1:51235639-51235658 | None:intergenic | 40.0% | |
| TGAACCATCATATCCAACCT+TGG | - | chr2.1:51235659-51235678 | MS.gene057423:CDS | 40.0% | |
| TGAGCTAAAGTTTGTGTTGG+TGG | + | chr2.1:51235636-51235655 | None:intergenic | 40.0% | |
| ! | AGCTAAAGTTTGTGTTGGTG+GGG | + | chr2.1:51235634-51235653 | None:intergenic | 40.0% |
| ! | GAGCTAAAGTTTGTGTTGGT+GGG | + | chr2.1:51235635-51235654 | None:intergenic | 40.0% |
| !! | AGCTATGAATTTGGTGGTGT+TGG | - | chr2.1:51235291-51235310 | MS.gene057423:intron | 40.0% |
| !! | TCTCCTGATCGATTTGACTT+AGG | + | chr2.1:51235393-51235412 | None:intergenic | 40.0% |
| !!! | AGCGACGAAAATTTTGAGAC+GGG | + | chr2.1:51235836-51235855 | None:intergenic | 40.0% |
| CTCGTGCCCAAAATCGTTAT+CGG | + | chr2.1:51235346-51235365 | None:intergenic | 45.0% | |
| ! | CTCAATTCAAACGGGCTTCT+AGG | + | chr2.1:51235240-51235259 | None:intergenic | 45.0% |
| !! | GAATTTGGTGGTGTTGGTCT+TGG | - | chr2.1:51235297-51235316 | MS.gene057423:intron | 45.0% |
| !! | GTTTTCGGCGAAAGTACAAC+CGG | - | chr2.1:51235192-51235211 | MS.gene057423:CDS | 45.0% |
| !! | TATGATGGCGGTGAAGAAAG+TGG | - | chr2.1:51235549-51235568 | MS.gene057423:CDS | 45.0% |
| !! | TGGTGTTGGTCTTGGAATTG+TGG | - | chr2.1:51235305-51235324 | MS.gene057423:intron | 45.0% |
| !! | TTTTCGGCGAAAGTACAACC+GGG | - | chr2.1:51235193-51235212 | MS.gene057423:CDS | 45.0% |
| TCCGATTGTGTCGAAAAGCC+CGG | + | chr2.1:51235214-51235233 | None:intergenic | 50.0% | |
| ! | ACCGGGCTTTTCGACACAAT+CGG | - | chr2.1:51235210-51235229 | MS.gene057423:CDS | 50.0% |
| ! | CGATAACGATTTTGGGCACG+AGG | - | chr2.1:51235344-51235363 | MS.gene057423:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 51235141 | 51236115 | 51235141 | ID=MS.gene057423 |
| chr2.1 | mRNA | 51235141 | 51236115 | 51235141 | ID=MS.gene057423.t1;Parent=MS.gene057423 |
| chr2.1 | exon | 51235511 | 51236115 | 51235511 | ID=MS.gene057423.t1.exon1;Parent=MS.gene057423.t1 |
| chr2.1 | CDS | 51235511 | 51236115 | 51235511 | ID=cds.MS.gene057423.t1;Parent=MS.gene057423.t1 |
| chr2.1 | exon | 51235141 | 51235288 | 51235141 | ID=MS.gene057423.t1.exon2;Parent=MS.gene057423.t1 |
| chr2.1 | CDS | 51235141 | 51235288 | 51235141 | ID=cds.MS.gene057423.t1;Parent=MS.gene057423.t1 |
| Gene Sequence |
| Protein sequence |
